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Computational study for suppression of CD25/IL-2 interaction Biol. Chem. (IF 3.27) Pub Date : 2020-11-19 Moein Dehbashi; Zohreh Hojati; Majid Motovali-bashi; Mazdak Ganjalikhani-Hakemi; Akihiro Shimosaka; William C. Cho
Cancer recurrence presents a huge challenge in cancer patient management. Immune escape is a key mechanism of cancer progression and metastatic dissemination. CD25 is expressed in regulatory T (Treg) cells including tumor-infiltrating Treg cells (TI-Tregs). These cells specially activate and reinforce immune escape mechanism of cancers. The suppression of CD25/IL-2 interaction would be useful against
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Frontmatter Biol. Chem. (IF 3.27) Pub Date : 2021-01-14
Journal Name: Biological Chemistry Volume: 402 Issue: 2 Pages: i-iii
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Irisin ameliorates endoplasmic reticulum stress and liver fibrosis through inhibiting PERK-mediated destabilization of HNRNPA1 in hepatic stellate cells Biol. Chem. (IF 3.27) Pub Date : 2021-01-12 Xin Liao; Wei Zhan; Rui Li; Tian Tian; Lei Yu; Qin Yang
Liver fibrosis is a common consequence of chronic liver diseases involved with the activation of hepatic stellate cells (HSCs) and endoplasmic reticulum (ER) stress. Irisin is a small polypeptide hormone that shows beneficial effects on metabolic disorders. The current study aimed to investigate the biological function of irisin on hepatic fibrosis. A mouse model of carbon tetrachloride (CCl4)-induced
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Identification of novel cardiovascular disease associated metabolites using untargeted metabolomics Biol. Chem. (IF 3.27) Pub Date : 2021-01-12 Shams Tabrez; Mohammed Razeeth Shait Mohammed; Nasimudeen R. Jabir; Mohammad Imran Khan
Cardiovascular disease (CVD) remains the leading cause of morbidity and mortality around the world. Early diagnosis of CVD could provide the opportunity for sensible management and better clinical outcome along with the prevention of further progression of the disease. In the current study, we used an untargeted metabolomic approach to identify possible metabolite(s) that associate well with the CVD
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Dynamics of Thiol-Based Redox Switches: Redox at its peak! Biol. Chem. (IF 3.27) Pub Date : 2021-01-12 Johannes M. Herrmann; Katja Becker; Tobias P. Dick
Journal Name: Biological Chemistry Volume: -1 Issue: ahead-of-print
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Prediction and analysis of redox-sensitive cysteines using machine learning and statistical methods Biol. Chem. (IF 3.27) Pub Date : 2021-01-06 Marcus Keßler; Ilka Wittig; Jörg Ackermann; Ina Koch
Reactive oxygen species are produced by a number of stimuli and can lead both to irreversible intracellular damage and signaling through reversible post-translational modification. It is unclear which factors contribute to the sensitivity of cysteines to redox modification. Here, we used statistical and machine learning methods to investigate the influence of different structural and sequence features
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Hsa-miR-186-5p regulates TGFβ signaling pathway through expression suppression of SMAD6 and SMAD7 genes in colorectal cancer Biol. Chem. (IF 3.27) Pub Date : 2021-01-06 Zahra Bayata; Zahra Ghaemia; Mehrdad Behmanesh; Bahram M. Soltani
TGFβ signaling is a known pathway to be involved in colorectal cancer (CRC) progression and miRNAs play crucial roles by regulating different components of this pathway. Hence, finding the link between miRNAs and the pathway could be beneficial for CRC therapy. Array data indicated that miR-186-5p is a differentially expressed miRNA in colorectal Tumor/Normal tissues and bioinformatics tools predicted
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Human glucose-dependent insulinotropic polypeptide (GIP) is an antimicrobial adjuvant re-sensitising multidrug-resistant Gram-negative bacteria Biol. Chem. (IF 3.27) Pub Date : 2021-01-06 Da’san M. M. Jaradat; Nehaya Al-Karablieh; Basmah H. M. Zaarer; Wenyi Li; Khalil K. Y. Saleh; Anas J. Rasras; Saeid Abu-Romman; Neil M. O’Brien-Simpson; John D. Wade
Increasing antibiotic resistance in Gram-negative bacteria has mandated the development of both novel antibiotics and alternative therapeutic strategies. Evidence of interplay between several gastrointestinal peptides and the gut microbiota led us to investigate potential and broad-spectrum roles for the incretin hormone, human glucose-dependent insulinotropic polypeptide (GIP) against the Enterobacteriaceae
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Vorinostat exhibits anticancer effects in triple-negative breast cancer cells by preventing nitric oxide-driven histone deacetylation Biol. Chem. (IF 3.27) Pub Date : 2021-01-06 Marianne B. Palczewski; Hannah Petraitis Kuschman; Rhea Bovee; Jason R. Hickok; Douglas D. Thomas
Triple-negative breast cancers (TNBC) that produce nitric oxide (NO) are more aggressive, and the expression of the inducible form of nitric oxide synthase (NOS2) is a negative prognostic indicator. In these studies, we set out to investigate potential therapeutic strategies to counter the tumor-permissive properties of NO. We found that exposure to NO increased proliferation of TNBC cells and that
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Regulation of DEAH-box RNA helicases by G-patch proteins Biol. Chem. (IF 3.27) Pub Date : 2021-01-06 Katherine E. Bohnsack; Ralf Ficner; Markus T. Bohnsack; Stefanie Jonas
RNA helicases of the DEAH/RHA family form a large and conserved class of enzymes that remodel RNA protein complexes (RNPs) by translocating along the RNA. Driven by ATP hydrolysis, they exert force to dissociate hybridized RNAs, dislocate bound proteins or unwind secondary structure elements in RNAs. The sub-cellular localization of DEAH-helicases and their concomitant association with different pathways
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Mtr4 RNA helicase structures and interactions Biol. Chem. (IF 3.27) Pub Date : 2021-01-06 Keith J. Olsen; Sean J. Johnson
Mtr4 is a Ski2-like RNA helicase that plays a central role in RNA surveillance and degradation pathways as an activator of the RNA exosome. Multiple crystallographic and cryo-EM studies over the past 10 years have revealed important insight into the Mtr4 structure and interactions with protein and nucleic acid binding partners. These structures place Mtr4 at the center of a dynamic process that recruits
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Detection of follicular regions in actin-stained whole slide images of the human lymph node by shock filter Biol. Chem. (IF 3.27) Pub Date : 2020-12-24 Patrick Wurzel; Jörg Ackermann; Hendrik Schäfer; Sonja Scharf; Martin-Leo Hansmann; Ina Koch
Human lymph nodes play a central part of immune defense against infection agents and tumor cells. Lymphoid follicles are compartments of the lymph node which are spherical, mainly filled with B cells. B cells are cellular components of the adaptive immune systems. In the course of a specific immune response, lymphoid follicles pass different morphological differentiation stages. The morphology and
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The DHX36-specific-motif (DSM) enhances specificity by accelerating recruitment of DNA G-quadruplex structures Biol. Chem. (IF 3.27) Pub Date : 2020-12-15 Bruce Chang-Gu; Devin Bradburn; Philip M. Yangyuoru; Rick Russell
DHX36 is a eukaryotic DEAH/RHA family helicase that disrupts G-quadruplex structures (G4s) with high specificity, contributing to regulatory roles of G4s. Here we used a DHX36 truncation to examine the roles of the 13-amino acid DHX36-specific motif (DSM) in DNA G4 recognition and disruption. We found that the DSM promotes G4 recognition and specificity by increasing the G4 binding rate of DHX36 without
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Dihydroartemisinin ameliorates balloon injury-induced neointimal formation through suppressing autophagy in vascular smooth muscle cells Biol. Chem. (IF 3.27) Pub Date : 2020-12-11 Xiaoyuan Wang; Junpeng Wu; Haiyang Zhang; Bei Sun; Renping Huang
The present study was designed to investigate the therapeutic effects of injection of dihydroartemisinin (DHA) into the balloon-injured carotid arteries on balloon injury-induced neointimal formation and to explore whether autophagy is involved in the action of DHA. Percutaneous transluminal balloon angioplasty was performed in Sprague-Dawley rats to induce neointimal formation, immediately after which
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HDAC8 promotes daunorubicin resistance of human acute myeloid leukemia cells via regulation of IL-6 and IL-8 Biol. Chem. (IF 3.27) Pub Date : 2020-12-11 Jieying Wu; Ling Zhang; Yashu Feng; Bijay Khadka; Zhigang Fang; Jiajun Liu
The chemoresistance is one of the major challenges for acute myeloid leukemia (AML) treatment. We found that the expression of histone deacetylase 8 (HDAC8) was increased in daunorubicin (DNR) resistant AML cells, while targeted inhibition of HDAC8 by its specific siRNA or inhibitor can restore sensitivity of DNR treatment . Further, targeted inhibition of HDAC8 can suppress expression of interleukin
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Apolipoprotein C-II and C-III preferably transfer to both HDL2 and the larger HDL3 from VLDL Biol. Chem. (IF 3.27) Pub Date : 2020-12-11 Azusa Yamazaki; Ryunosuke Ohkawa; Yuka Yamagata; Yuna Horiuchi; Shao-Jui Lai; Takahiro Kameda; Naoya Ichimura; Shuji Tohda; Minoru Tozuka
Triglyceride hydrolysis by lipoprotein lipase (LPL), regulated by apolipoproteins C-II (apoC-II) and C-III (apoC-III), is essential for maintaining normal lipid homeostasis. During triglyceride lipolysis, the apoCs are known to be transferred from very low-density lipoprotein (VLDL) to high-density lipoprotein (HDL), but the detailed mechanisms of this transfer remain unclear. In this study, we investigated
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Transcription, translation, and DNA repair: new insights from emerging noncanonical substrates of RNA helicases Biol. Chem. (IF 3.27) Pub Date : 2020-12-14 Matthew P. Russon; Kirsten M. Westerhouse; Elizabeth J. Tran
RNA helicases are enzymes that exist in all domains of life whose canonical functions include ATP-dependent remodeling of RNA structures and displacement of proteins from ribonucleoprotein complexes (RNPs). These enzymes play roles in virtually all processes of RNA metabolism, including pre-mRNA splicing, rRNA processing, nuclear mRNA export, translation and RNA decay. Here we review emerging noncanonical
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Decreased level of miR-1301 promotes colorectal cancer progression via activation of STAT3 pathway Biol. Chem. (IF 3.27) Pub Date : 2020-11-25 Fangfang Yang; Hua Wang; Bianbian Yan; Tong Li; Lulu Min; Erfei Chen; Jin Yang
The molecular pathogenesis of colorectal cancer (CRC) has been widely investigated in recent years. Accumulating evidence has indicated that microRNA (miRNA) dysregulation participates in the processes of driving CRC initiation and progression. Aberrant expression of miR-1301 has been found in various tumor types. However, its role in CRC remains to be elucidated. In the present study, we identified
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Integrated Ca2+ flux and AFM force analysis in human iPSC-derived cardiomyocytes Biol. Chem. (IF 3.27) Pub Date : 2020-10-27 Andrey V. Malkovskiy; Nadezda Ignatyeva; Yuanyuan Dai; Gerd Hasenfuss; Jayakumar Rajadas; Antje Ebert
We developed a new approach for combined analysis of calcium (Ca2+) handling and beating forces in contractile cardiomyocytes. We employed human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) from dilated cardiomyopathy (DCM) patients carrying an inherited mutation in the sarcomeric protein troponin T (TnT), and isogenic TnT-KO iPSC-CMs generated via CRISPR/Cas9 gene editing. In these
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Influence of monovalent metal ions on metal binding and catalytic activity of the 10–23 DNAzyme Biol. Chem. (IF 3.27) Pub Date : 2020-09-30 Hannah Rosenbach; Jan Borggräfe; Julian Victor; Christine Wuebben; Olav Schiemann; Wolfgang Hoyer; Gerhard Steger; Manuel Etzkorn; Ingrid Span
Deoxyribozymes (DNAzymes) are single-stranded DNA molecules that catalyze a broad range of chemical reactions. The 10–23 DNAzyme catalyzes the cleavage of RNA strands and can be designed to cleave essentially any target RNA, which makes it particularly interesting for therapeutic and biosensing applications. The activity of this DNAzyme in vitro is considerably higher than in cells, which was suggested
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RNA secondary structure dependence in METTL3–METTL14 mRNA methylation is modulated by the N-terminal domain of METTL3 Biol. Chem. (IF 3.27) Pub Date : 2020-10-19 Nathalie Meiser; Nicole Mench; Martin Hengesbach
N6-methyladenosine (m6A) is the most abundant modification in mRNA. The core of the human N6-methyltransferase complex (MTC) is formed by a heterodimer consisting of METTL3 and METTL14, which specifically catalyzes m6A formation within an RRACH sequence context. Using recombinant proteins in a site-specific methylation assay that allows determination of quantitative methylation yields, our results
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Kill one or kill the many: interplay between mitophagy and apoptosis Biol. Chem. (IF 3.27) Pub Date : 2020-10-12 Simone Wanderoy; J. Tabitha Hees; Ramona Klesse; Frank Edlich; Angelika B. Harbauer
Mitochondria are key players of cellular metabolism, Ca2+ homeostasis, and apoptosis. The functionality of mitochondria is tightly regulated, and dysfunctional mitochondria are removed via mitophagy, a specialized form of autophagy that is compromised in hereditary forms of Parkinson’s disease. Through mitophagy, cells are able to cope with mitochondrial stress until the damage becomes too great, which
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Genomic insights into cyanobacterial protein translocation systems Biol. Chem. (IF 3.27) Pub Date : 2020-10-12 David A. Russo; Julie A. Z. Zedler
Cyanobacteria are ubiquitous oxygenic photosynthetic bacteria with a versatile metabolism that is highly dependent on effective protein targeting. Protein sorting in diderm bacteria is not trivial and, in cyanobacteria, even less so due to the presence of a complex membrane system: the outer membrane, the plasma membrane and the thylakoid membrane. In cyanobacteria, protein import into the thylakoids
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The role of very long chain fatty acids in yeast physiology and human diseases Biol. Chem. (IF 3.27) Pub Date : 2020-11-23 Pia Erdbrügger; Florian Fröhlich
Fatty acids (FAs) are a highly diverse class of molecules that can have variable chain length, number of double bonds and hydroxylation sites. FAs with 22 or more carbon atoms are described as very long chain fatty acids (VLCFAs). VLCFAs are synthesized in the endoplasmic reticulum (ER) through a four-step elongation cycle by membrane embedded enzymes. VLCFAs are precursors for the synthesis of sphingolipids
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LOTUS-domain proteins - developmental effectors from a molecular perspective Biol. Chem. (IF 3.27) Pub Date : 2020-11-04 Jana Kubíková; Rebecca Reinig; Harpreet Kaur Salgania; Mandy Jeske
The LOTUS domain (also known as OST-HTH) is a highly conserved protein domain found in a variety of bacteria and eukaryotes. In animals, the LOTUS domain is present in the proteins Oskar, TDRD5/Tejas, TDRD7/TRAP/Tapas, and MARF1/Limkain B1, all of which play essential roles in animal development, in particular during oogenesis and/or spermatogenesis. This review summarizes the diverse biological as
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Highlight: young research groups in Germany – 3rd edition Biol. Chem. (IF 3.27) Pub Date : 2020-11-06 Jürgen Lassak; Andreas Schlundt
Journal Name: Biological Chemistry Volume: 402 Issue: 1 Pages: 5-6
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Douglas D. Thomas named next Editor-in-Chief of Biological Chemistry Biol. Chem. (IF 3.27) Pub Date : 2020-11-25 Bernhard Brüne
Journal Name: Biological Chemistry Volume: 402 Issue: 1 Pages: 3-4
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Publisher’s Note Biol. Chem. (IF 3.27) Pub Date : 2020-12-02
Journal Name: Biological Chemistry Volume: 402 Issue: 1 Pages: 1-1
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Frontmatter Biol. Chem. (IF 3.27) Pub Date : 2020-12-03
Journal Name: Biological Chemistry Volume: 402 Issue: 1 Pages: i-iii
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Lysine acetyltransferase Tip60 acetylates the APP adaptor Fe65 to increase its transcriptional activity Biol. Chem. (IF 3.27) Pub Date : 2020-12-01 Sabine Probst; Florian Riese; Larissa Kägi; Maik Krüger; Natalie Russi; Roger M. Nitsch; Uwe Konietzko
Proteolytic processing of the amyloid precursor protein (APP) releases the APP intracellular domain (AICD) from the membrane. Bound to the APP adaptor protein Fe65 and the lysine acetyltransferase (KAT) Tip60, AICD translocates to the nucleus. Here, the complex forms spherical condensates at sites of endogenous target genes, termed AFT spots (AICD-Fe65-Tip60). We show that loss of Tip60 KAT activity
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Targeting spectrin redox switches to regulate the mechanoproperties of red blood cells Biol. Chem. (IF 3.27) Pub Date : 2020-12-01 Frederik Barbarino; Lucas Wäschenbach; Virginia Cavalho-Lemos; Melissa Dillenberger; Katja Becker; Holger Gohlke; Miriam M. Cortese-Krott
The mechanical properties of red blood cells (RBCs) are fundamental for their physiological role as gas transporters. RBC flexibility and elasticity allow them to survive the hemodynamic changes in the different regions of the vascular tree, to dynamically contribute to the flow thereby decreasing vascular resistance, and to deform during the passage through narrower vessels. RBC mechanoproperties
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Regulation of RNA stability at the 3′ end Biol. Chem. (IF 3.27) Pub Date : 2020-11-26 Mallory I. Frederick; Ilka U. Heinemann
RNA homeostasis is regulated by a multitude of cellular pathways. Although the addition of untemplated adenine residues to the 3′ end of mRNAs has long been known to affect RNA stability, newly developed techniques for 3′-end sequencing of RNAs have revealed various unexpected RNA modifications. Among these, uridylation is most recognized for its role in mRNA decay but is also a key regulator of numerous
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Shifting paradigms and novel players in Cys-based redox regulation and ROS signaling in plants - and where to go next Biol. Chem. (IF 3.27) Pub Date : 2020-11-27 Andreas J. Meyer; Anna Dreyer; José M. Ugalde; Elias Feitosa-Araujo; Karl-Josef Dietz; Markus Schwarzländer
Cys-based redox regulation was long regarded a major adjustment mechanism of photosynthesis and metabolism in plants, but in the recent years, its scope has broadened to most fundamental processes of plant life. Drivers of the recent surge in new insights into plant redox regulation have been the availability of the genome-scale information combined with technological advances such as quantitative
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Thiol-based redox switches in the major pathogen Staphylococcus aureus Biol. Chem. (IF 3.27) Pub Date : 2020-11-23 Nico Linzner; Vu Van Loi; Verena Nadin Fritsch; Haike Antelmann
Staphylococcus aureus is a major human pathogen, which encounters reactive oxygen, nitrogen, chlorine, electrophile and sulfur species (ROS, RNS, RCS, RES and RSS) by the host immune system, during cellular metabolism or antibiotics treatments. To defend against redox active species and antibiotics, S. aureus is equipped with redox sensing regulators that often use thiol switches to control the expression
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Specific acclimations to phosphorus limitation in the marine diatom Phaeodactylum tricornutum. Biol. Chem. (IF 3.27) Pub Date : 2020-09-14 Gianluca Dell'Aquila,Uwe G Maier
Phosphorus (P) is a crucial element and diatoms, unicellular phototrophic organisms, evolved efficient strategies to handle limiting phosphorus concentrations in the oceans. In the last decade, several groups investigated the model diatom Phaeodactylum tricornutum concerning phosphate homeostasis mechanisms. Here, we summarize the actual status of knowledge by linking the available data sets, thereby
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Metabolism of non-growing bacteria. Biol. Chem. (IF 3.27) Pub Date : 2020-09-11 Martin Lempp,Paul Lubrano,Gert Bange,Hannes Link
A main function of bacterial metabolism is to supply biomass building blocks and energy for growth. This seems to imply that metabolism is idle in non-growing bacteria. But how relevant is metabolism for the physiology of non-growing bacteria and how active is their metabolism? Here, we reviewed literature describing metabolism of non-growing bacteria in their natural environment, as well as in biotechnological
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Unexpected metabolic versatility among type II methanotrophs in the Alphaproteobacteria. Biol. Chem. (IF 3.27) Pub Date : 2020-11-09 Anna Hakobyan,Werner Liesack
Aerobic methane-oxidizing bacteria, or methanotrophs, play a crucial role in the global methane cycle. Their methane oxidation activity in various environmental settings has a great mitigation effect on global climate change. Alphaproteobacterial methanotrophs were among the first to be taxonomically characterized, nowadays unified in the Methylocystaceae and Beijerinckiaceae families. Originally thought
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Biochemical unity revisited: microbial central carbon metabolism holds new discoveries, multi-tasking pathways, and redundancies with a reason Biol. Chem. (IF 3.27) Pub Date : 2020-10-02 Lennart Schada von Borzyskowski; Iria Bernhardsgrütter; Tobias J. Erb
For a long time, our understanding of metabolism has been dominated by the idea of biochemical unity, i.e., that the central reaction sequences in metabolism are universally conserved between all forms of life. However, biochemical research in the last decades has revealed a surprising diversity in the central carbon metabolism of different microorganisms. Here, we will embrace this biochemical diversity
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Glutaredoxins and iron-sulfur protein biogenesis at the interface of redox biology and iron metabolism Biol. Chem. (IF 3.27) Pub Date : 2020-10-08 Ulrich Mühlenhoff; Joseph J. Braymer; Stefan Christ; Nicole Rietzschel; Marta A. Uzarska; Benjamin D. Weiler; Roland Lill
The physiological roles of the intracellular iron and redox regulatory systems are intimately linked. Iron is an essential trace element for most organisms, yet elevated cellular iron levels are a potent generator and amplifier of reactive oxygen species and redox stress. Proteins binding iron or iron-sulfur (Fe/S) clusters, are particularly sensitive to oxidative damage and require protection from
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Diversity of GPI-anchored fungal adhesins Biol. Chem. (IF 3.27) Pub Date : 2020-10-09 Lars-Oliver Essen; Marian Samuel Vogt; Hans-Ulrich Mösch
Selective adhesion of fungal cells to one another and to foreign surfaces is fundamental for the development of multicellular growth forms and the successful colonization of substrates and host organisms. Accordingly, fungi possess diverse cell wall-associated adhesins, mostly large glycoproteins, which present N-terminal adhesion domains at the cell surface for ligand recognition and binding. In order
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Multicellular and unicellular responses of microbial biofilms to stress Biol. Chem. (IF 3.27) Pub Date : 2020-10-02 Daniel K.H. Rode; Praveen K. Singh; Knut Drescher
Biofilms are a ubiquitous mode of microbial life and display an increased tolerance to different stresses. Inside biofilms, cells may experience both externally applied stresses and internal stresses that emerge as a result of growth in spatially structured communities. In this review, we discuss the spatial scales of different stresses in the context of biofilms, and if cells in biofilms respond to
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Generating asymmetry in a changing environment: cell cycle regulation in dimorphic alphaproteobacteria Biol. Chem. (IF 3.27) Pub Date : 2020-09-24 Muriel C. F. van Teeseling; Martin Thanbichler
While many bacteria divide by symmetric binary fission, some alphaproteobacteria have strikingly asymmetric cell cycles, producing offspring that differs significantly in their morphology and reproductive state. To establish this asymmetry, these species employ a complex cell cycle regulatory pathway based on two-component signaling cascades. At the center of this network is the essential DNA-binding
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Cyclic di-GMP signaling controlling the free-living lifestyle of alpha-proteobacterial rhizobia Biol. Chem. (IF 3.27) Pub Date : 2020-10-02 Elizaveta Krol; Simon Schäper; Anke Becker
Cyclic-di-GMP (c-di-GMP) is a ubiquitous bacterial second messenger which has been associated with a motile to sessile lifestyle switch in many bacteria. Here, we review recent insights into c-di-GMP regulated processes related to environmental adaptations in alphaproteobacterial rhizobia, which are diazotrophic bacteria capable of fixing nitrogen in symbiosis with their leguminous host plants. The
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Spatial organisation enhances versatility and specificity in cyclic di-GMP signaling. Biol. Chem. (IF 3.27) Pub Date : 2020-10-15 Sandra Kunz,Peter L Graumann
The second messenger cyclic di-GMP regulates a variety of processes in bacteria, many of which are centered around the decision whether to adopt a sessile or a motile life style. Regulatory circuits include pathogenicity, biofilm formation, and motility in a wide variety of bacteria, and play a key role in cell cycle progression in Caulobacter crescentus. Interestingly, multiple, seemingly independent
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Physiology of guanosine-based second messenger signaling in Bacillus subtilis. Biol. Chem. (IF 3.27) Pub Date : 2020-09-16 Gert Bange,Patricia Bedrunka
The guanosine-based second messengers (p)ppGpp and c-di-GMP are key players of the physiological regulation of the Gram-positive model organism Bacillus subtilis. Their regulatory spectrum ranges from key metabolic processes over motility to biofilm formation. Here we review our mechanistic knowledge on their synthesis and degradation in response to environmental and stress signals as well as what
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Masters of change. Biol. Chem. (IF 3.27) Pub Date : 2020-10-01 Erhard Bremer
Journal Name: Biological Chemistry Volume: 401 Issue: 12 Pages: 1305-1306
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Frontmatter Biol. Chem. (IF 3.27) Pub Date : 2020-11-09
Journal Name: Biological Chemistry Volume: 401 Issue: 12 Pages: i-iii
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Thioredoxin-dependent control balances the metabolic activities of tetrapyrrole biosynthesis Biol. Chem. (IF 3.27) Pub Date : 2020-10-27 Daniel Wittmann; Neha Sinha; Bernhard Grimm
Plastids are specialized organelles found in plants, which are endowed with their own genomes, and differ in many respects from the intracellular compartments of organisms belonging to other kingdoms of life. They differentiate into diverse, plant organ-specific variants, and are perhaps the most versatile organelles known. Chloroplasts are the green plastids in the leaves and stems of plants, whose
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Thiol switches in membrane proteins - Extracellular redox regulation in cell biology Biol. Chem. (IF 3.27) Pub Date : 2020-10-28 Inken Lorenzen; Johannes A. Eble; Eva-Maria Hanschmann
Redox-mediated signal transduction depends on the enzymatic production of second messengers such as hydrogen peroxide, nitric oxide and hydrogen sulfite, as well as specific, reversible redox modifications of cysteine-residues in proteins. So-called thiol switches induce for instance conformational changes in specific proteins that regulate cellular pathways e.g., cell metabolism, proliferation, migration
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3-Mercaptopyruvate sulfurtransferase: an enzyme at the crossroads of sulfane sulfur trafficking Biol. Chem. (IF 3.27) Pub Date : 2020-10-20 Brandán Pedre; Tobias P. Dick
3-Mercaptopyruvate sulfurtransferase (MPST) catalyzes the desulfuration of 3-mercaptopyruvate to generate an enzyme-bound hydropersulfide. Subsequently, MPST transfers the persulfide’s outer sulfur atom to proteins or small molecule acceptors. MPST activity is known to be involved in hydrogen sulfide generation, tRNA thiolation, protein urmylation and cyanide detoxification. Tissue-specific changes
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Emerging roles for non-selenium containing ER-resident glutathione peroxidases in cell signaling and disease Biol. Chem. (IF 3.27) Pub Date : 2020-10-22 Katalin Buday; Marcus Conrad
Maintenance of cellular redox control is pivotal for normal cellular functions and cell fate decisions including cell death. Among the key cellular redox systems in mammals, the glutathione peroxidase (GPX) family of proteins is the largest conferring multifaceted functions and affecting virtually all cellular processes. The endoplasmic reticulum (ER)-resident GPXs, designated as GPX7 and GPX8, are
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PCAT1 induced by transcription factor YY1 promotes cholangiocarcinoma proliferation, migration and invasion by sponging miR-216a-3p to up-regulate oncogene BCL3 Biol. Chem. (IF 3.27) Pub Date : 2020-10-23 Dongsheng Sun; Yuqiao Zhao; Weina Wang; Canghai Guan; Zengtao Hu; Lang Liu; Xingming Jiang
This study was designed to illustrate the function and role of PCAT1 in CCA. The relative expression was confirmed by RT-qPCR and western blot. The biological function of PCAT1 was evaluated by CCK8, EdU, colony formation, wound healing, transwell, and subcutaneous tumor formation assays. Protein levels of EMT markers were measured by western blot. The binding relationship was predicted by JASPAR and
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Morphological dependent effect of cell-free formed supramolecular fibronectin on cellular activities Biol. Chem. (IF 3.27) Pub Date : 2020-10-19 Phong Le; Hoang-Nghi Mai-Thi; Volker R. Stoldt; Ngoc Quyen Tran; Khon Huynh
Fibrillar fibronectin (FFN), an active form of fibronectin (FN), plays important roles in various cellular processes. Our goal is to investigate effect of FFN morphology on cellular behaviors. Plasma FN at two concentrations was cross-linked into FFN by dialysis against 2 M urea followed by morphological analysis under Scanning Electron Microscopy. To evaluate effect of FFN morphology, fibroblasts
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Expansion and inflammation of white adipose tissue - focusing on adipocyte progenitors Biol. Chem. (IF 3.27) Pub Date : 2020-10-16 Wenjing Liu; Dahui Li; Handi Cao; Haoyun Li; Yu Wang
Adipose tissue is an important organ in our body, participating not only in energy metabolism but also immune regulation. It is broadly classified as white (WAT) and brown (BAT) adipose tissues. WAT is highly heterogeneous, composed of adipocytes, various immune, progenitor and stem cells, as well as the stromal vascular populations. The expansion and inflammation of WAT are hallmarks of obesity and
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Individual and combined effects of GIP and xenin on differentiation, glucose uptake and lipolysis in 3T3-L1 adipocytes. Biol. Chem. (IF 3.27) Pub Date : 2020-08-26 Andrew English,Sarah L Craig,Peter R Flatt,Nigel Irwin
The incretin hormone glucose-dependent insulinotropic polypeptide (GIP), released postprandially from K-cells, has established actions on adipocytes and lipid metabolism. In addition, xenin, a related peptide hormone also secreted from K-cells after a meal, has postulated effects on energy regulation and lipid turnover. The current study has probed direct individual and combined effects of GIP and
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Hsp70-mediated quality control: should I stay or should I go? Biol. Chem. (IF 3.27) Pub Date : 2020-08-04 Verena Kohler,Claes Andréasson
Chaperones of the 70 kDa heat shock protein (Hsp70) superfamily are key components of the cellular proteostasis system. Together with its co-chaperones, Hsp70 forms proteostasis subsystems that antagonize protein damage during physiological and stress conditions. This function stems from highly regulated binding and release cycles of protein substrates, which results in a flow of unfolded, partially
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Redox-dependent and independent effects of thioredoxin interacting protein. Biol. Chem. (IF 3.27) Pub Date : 2020-08-31 Xiankun Cao,Wenxin He,Yichuan Pang,Yu Cao,An Qin
Thioredoxin interacting protein (TXNIP) is an important physiological inhibitor of the thioredoxin (TXN) redox system in cells. Regulation of TXNIP expression and/or activity not only plays an important role in redox regulation but also exerts redox-independent physiological effects that exhibit direct pathophysiological consequences including elevated inflammatory response, aberrant glucose metabolism
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The role of mitochondrial ATP synthase in cancer. Biol. Chem. (IF 3.27) Pub Date : 2020-08-24 Chiara Galber,Manuel Jesus Acosta,Giovanni Minervini,Valentina Giorgio
The mitochondrial ATP synthase is a multi-subunit enzyme complex located in the inner mitochondrial membrane which is essential for oxidative phosphorylation under physiological conditions. In this review, we analyse the enzyme functions involved in cancer progression by dissecting specific conditions in which ATP synthase contributes to cancer development or metastasis. Moreover, we propose the role
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Frontmatter Biol. Chem. (IF 3.27) Pub Date : 2020-10-12
Journal Name: Biological Chemistry Volume: 401 Issue: 11 Pages: i-iii
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Genetically encoded thiol redox-sensors in the zebrafish model: lessons for embryonic development and regeneration Biol. Chem. (IF 3.27) Pub Date : 2020-10-06 Oksana Breus; Thomas Dickmeis
Important roles for reactive oxygen species (ROS) and redox signaling in embryonic development and regenerative processes are increasingly recognized. However, it is difficult to obtain information on spatiotemporal dynamics of ROS production and signaling in vivo. The zebrafish is an excellent model for in vivo bioimaging and possesses a remarkable regenerative capacity upon tissue injury. Here, we
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