样式: 排序: IF: - GO 导出 标记为已读
-
Employing soil isolated fungi for production of bioactive phenolic compounds: a fermentative approach Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2024-03-13 Rathnaprabha Dharavath, Srividya A.
An efficient method of solid-state fermentation (SSF) is reported for producing bioactive phenolic compounds using soil-isolated fungi. Antioxidant activity using a rapid DPPH (1,1-diphenyl-2-picry...
-
Recombinant expression and purification of phenylalanyl-tRNA synthetase from wheat: a long-lasting poly(U)-dependent poly(Phe) synthesis system Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2024-03-05 Haruyuki Furukawa, Yuto Nagashio, Kensuke Tsutsumi, Naofumi Matsubara, Ryohei Kato, Chie Tomikawa, Kazuyuki Takai
Synthetic genes for the two subunits of phenylalanyl-tRNA synthetase (PheRS) from wheat were expressed in Escherichia coli. When each gene was induced individually, the α subunit with a cleavable 6...
-
Production, purification, properties and current perspectives for modification and application of microbial lipases Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2024-03-06 Junxin Zhao, Maomao Ma, Zheling Zeng, Dongman Wan, Xianghui Yan, Jiaheng Xia, Ping Yu, Deming Gong
With the industrialization and development of modern science, the application of enzymes as green and environmentally friendly biocatalysts in industry has been increased widely. Among them, lipase...
-
Tyrosinase from the pulps of local cultivars of Musa spp: Purification, characterization, immobilization, and application in the batch production of l-3,4-dihydroxyphenylalanine Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2024-03-06 Muinat Moronke Adeyanju, Adedeji Nelson Ademakinwa
Tyrosinase, an enzyme involved in browning reactions in plants/crops exposed to mechanical injury, was isolated from the pulp of some different locally available bananas (M. cavendish, M. acuminata...
-
Optimization of total flavonoids purification process in rose by uniform design method Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2024-02-09 Dongmei Qin, Cui He, Yuefeng Gao, Bo Lyu
This study aims to establish a method for purifying total flavonoids in roses using macroporous resin columns, intending to leverage and harness their potential. We screened six macroporous resins ...
-
Fractional mixture design: a novel design and methodology for analyzing and optimizing mixtures of several ingredients Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2024-01-19 José Luis Navarrete Bolaños
Mixture designs are employed to systematically change the composition of mixtures and investigate how those changes impact their properties. However, all mixture designs currently available are imp...
-
Recombinant proteins production in Escherichia coli BL21 for vaccine applications: a cost estimation of potential industrial-scale production scenarios Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2024-01-10 Ilkgul Akmayan, Abdullah Bilal Ozturk, Tulin Ozbek
Recent SARS-CoV-2 pandemic elevated research interest in microorganism-related diseases, and protective health application importance such as vaccination and immune promoter agents emerged. Among t...
-
β-glucosidases from Saccharomyces cerevisiae: production, protein precipitation, characterization, and application in the enzymatic hydrolysis of delignified sugarcane bagasse Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2024-01-05 Geise Camila de Araujo Ribeiro, Sandra Aparecida de Assis
β-glucosidase is an essential enzyme for the enzymatic hydrolysis of lignocellulosic biomass, as it catalyzes the final stage of cellulose breakdown, releasing glucose. This paper aims to produce β...
-
Purification and biochemical characterization of novel α-amylase and cellulase from Bacillus sp. PM06 Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-12-23 Rekha Rajesh, Sathyanarayana N. Gummadi
Bacillus sp. PM06, previously isolated from sugarcane waste pressmud, could produce dual enzymes α-amylase and cellulase. The isolate’s crude enzymes were purified homogeneously using ammonium sulf...
-
Choice of gDNA isolation method has a significant impact on average murine Telomere Length estimates Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-12-13 E. Kidd, E. Meimaridou, J. Williams, L. A. Metherell, A. J. Walley, U. L. Fairbrother
Telomere Length (TL) and integrity is significantly associated with age-related disease, multiple genetic and environmental factors. We observe mouse genomic DNA (gDNA) isolation methods to have a ...
-
Mitigating membrane fouling in an internal loop airlift membrane photobioreactor containing Spirulina platensis: effects of riser cross-sectional area and hydrophilic baffles Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-11-27 Tarlan Nasser, Mir Mehrshad Emamshoushtari, Salar Helchi, Ardeshir Saeidi, Farshid Pajoum Shariati
Membrane photobioreactors (MPBRs) have gained significant attention due to their ability to support microalgae activities such as cultivation, harvesting, and production of beneficial products. Des...
-
A comparative study on the influence of equipment design on the efficiency of dynamic maceration of Azadirachta excelsa leaves Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-11-21 Kahviyaah Letchumanan, Nor Hayati Abdullah, Azila Abdul-Aziz
Dynamic maceration facilitates diffusion in solid-liquid extraction through controlling temperature and providing agitation. However, equipment design for dynamic maceration in previous investigati...
-
Improving the production of recombinant L-Asparaginase-II in Escherichia coli by co-expressing catabolite repressor activator (cra) gene Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-11-16 Debashrita Mittra, Shubhashree Mahalik
Identification of a single genetic target for microbial strain improvement is difficult due to the complexity of the genetic regulatory network. Hence, a more practical approach is to identify bott...
-
Analysis of Aureobasidium pullulans LB83 secretome reveals distinct carbohydrate active enzymes for biomass saccharification Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-11-15 Matheus Maitan Vieira, Fernanda Lima Valadares, Josman Velasco, Silvio S da Silva, Fernando Segato, Anuj K Chandel
Aureobasidium pullulans LB83 is a versatile biocatalyst that produces a plethora of bioactive products thriving on a variety of feedstocks under the varying culture conditions. In our last study us...
-
Extraction of phenolic compounds from walnut green husk (Juglans regia L.) by Salting-Out extraction method Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-11-10 Sorour Barekat, Ali Nasirpour, Javad Keramat, Mohammad Dinari, Ali Sedaghat Doost, Paul Van der Meeren
Some factors in the salting-out extraction (SOE) method play a major role. The aim of this study was to investigate the interaction effects of the phase forming components and consequently select t...
-
Purification and characterization of extracellular tannase from Aspergillus fumigatus MA using Syzigium cumini leaves under solid state fermentation Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-11-10 Krishan Kumar Selwal, Manjit K. Selwal
This study reports the tannase purification produced by a tannery effluent-originated fungal isolate i.e., Aspergillus fumigatus MA under solid state fermentation (SSF) condition. Purification of t...
-
Expression and characterization of an organic solvent tolerant recombinant lipase from Staphylococcus capitis SH6 for food wastewater treatment Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-11-08 Fatma Rmili, Najeh Krayem, Celine Loiseau, Laurent Gauvry, Fakher Frikha, Françoise Ergan, Mohamed Chamkha, Adel Sayari, Ahmed Fendri
The study illustrated here aims on an organic solvent tolerant lipase from Staphylococcus capitis (SCL). The gene part, encoding the mature lipase, was cloned and sequenced. The concluded polypepti...
-
Enhancement of bacterial cellulose production by ethanol and lactic acid by using Gluconacetobacter kombuchae Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-11-08 Poonam Sharma, Ritu Sharma, Simran Ahuja, Anita Yadav, Sanjiv Arora, Neeraj K. Aggarwal
The current study intended to analyze the impact of ethanol and lactic acid on the bacterial cellulose yield as well as physicochemical and mechanical properties, by using Gluconacetobacter kombuch...
-
Kinetics of dye decolorization using heterogeneous catalytic system with immobilized Achromobacter xylosoxidans DDB6 Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-11-01 B. S. Harish, Thangavelu Thayumanavan, Rathinasamy Subashkumar, K. Gopal, N. Kowsik Raj
Textile effluents containing toxic dyes must be treated effectively before discharge to prevent adverse environmental impacts. Traditional physical and chemical treatment methods are costly and gen...
-
Subcritical water extraction of polysaccharides from Gastrodiae Rhizoma: optimization, characterization and in vitro hepatoprotective activity Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-10-20 Guozhen Wu, Hongjing Dong, Minggang Ding, Xiao Wang
Subcritical water extraction (SWE) is an efficient and eco-friendly technology that rapidly extracts valuable compounds from natural materials. In this study, response surface methodology (RSM) was...
-
Preparing the pure lignin peroxidase and exploring the effects of chemicals on the activity Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-10-16 Shouzhi Li, Lu He, Na Shi, Yanzhen Chen, Muhammad Saeed, Zhong Ni, Huayou Chen
Heterogous expression of lignin peroxidase (LiP) from Phanerochaete chrysosporium was performed in by E. coli prokaryotic expression system, and pure LiP was prepared by washing, refolding, and pur...
-
Extraction of collagenolytic proteases from Aspergillus heteromorphus URM 0269 in an aqueous two-phase system for application in collagen hydrolysis Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-10-10 Beatriz de Aquino Marques da Costa, Ana Cláudia Vaz de Araújo, Lígia Maria Gonçalves Fernandes, Ana Lúcia Figueiredo Porto, Vagne de Melo Oliveira, Tatiana Souza Porto
Collagenolytic proteases produced by Aspergillus heteromorphus URM0269 were extracted using a PEG/sulfate aqueous two-phase system (ATPS). A 23 factorial design was performed to analyze the indepen...
-
Production, characterization, and bio-ethanologenic potential of a novel tripartite raw starch-digesting amylase from Priestia flexa UCCM 00132 Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-10-03 David Sam Ubi, Maurice George Ekpenyong, Eloghosa Joyce Ikharia, Ernest Ablewho Akwagiobe, Atim David Asitok, Sylvester Peter Antai
The biological conversion of agro-waste biomass into value-added metabolites is one of the trendy biotechnological research areas in recent times. One of the major drawbacks of the bioprocess is th...
-
Multi-functional xylanase from Aspergillus sydowii: biosynthesis of nanoconjugates, optimization by Taguchi approach and biodeinking potential Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-09-29 J. A. Elegbede, A. Lateef, E. B. Gueguim-Kana, L. S. Beukes, N. Matyumza
The search for effective production of xylanase which is an important industrial enzyme led to the present study that explored xylanase production by Aspergillus sydowii SF through Taguchi optimiza...
-
High-level production of poly-γ-glutamic acid by a newly isolated Bacillus sp. YJY-8 and potential use in increasing the production of tomato Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-09-28 Fuming He, Baojun Gao, Xin Cheng, Jiao Zhai, Xinqing Zhang, Chuanlun Yang, Tian Jiewei
Strain YJY-8, a new γ-polyglutamic acid producer, was separated from fermented soybean paste samples. The strain was identified as a genus of Bacillus by morphological and 16S rDNA sequence analysi...
-
Utilization of the sugar fraction from Arabica coffee pulp as a carbon source for bacteria producing cellulose and cytotoxicity with human keratinocyte Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-09-25 Jiraporn Sangta, Warintorn Ruksiriwanich, Chuda Chittasupho, Korawan Sringarm, Pornchai Rachtanapun, Cassie Bakshani, William Willats, Sarana Sommano
Coffee pulp (CP), a by-product of coffee production, is an underutilized resource with significant potential value. CP contains monosaccharides that can serve as an ideal carbon source for bacteria...
-
Optimization and purification of laccase activity from Mammaliicoccus sciuri isolated from the soils of Similipal, Odisha, India: a kinetics study of crystal violet dye decolorization Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-09-20 Monalisa Mahuri, Monalisa Mohanty, Hrudayanath Thatoi
Four laccase-producing bacteria were found in soil samples from the Similipal Biosphere Reserve in Odisha, according to the current study. The isolates (SLCB1 to SLCB4) were evaluated for their lac...
-
Production of tannase from a newly isolated yeast, Geotrichum cucujoidarum using agro-residues Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-09-12 Nishanthini Thangavelu, Jothika Jeyabalan, Ajithkumar Veluchamy, Prasanna D. Belur
Abstract With an aim of producing commercially important tannase enzyme using cheap and readily available agro-residues, leaves of Indian Gooseberry (Phyllanthus emblica) and Jamun (Syzygium cumini), peels of Lemon (Citrus limon), and Pomegranate (Punica granatum) were screened. Newly isolated Geotrichum cucujoidarum was utilized for the study. Preliminary studies indicated that tannase titer obtained
-
PEGylation versus glycosylation: effect on the thermodynamics and thermostability of crisantaspase Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-09-12 Karin Torres‐Obreque, Eduardo Krebs Kleingesinds, João H. P. M. Santos, Gustavo Carretero, Jheniffer Rabelo, Attilio Converti, Gisele Monteiro, Adalberto Pessoa Jr, Carlota O. Rangel-Yagui
Abstract Thermostability is an important and desired feature of therapeutic proteins and is critical for the success or failure of protein drugs development. It can be increased by PEGylation—binding of poly(ethylene glycol) moieties—or glycosylation—post-translational modification to add glycans. Here, the thermostability and thermodynamic parameters of native, PEGylated, and glycosylated versions
-
Enhancing food preservation with postbiotic metabolites γ-aminobutyric acid (GABA) and bacteriocin-like inhibitory substances (BLIS) produced by Lactobacillus brevis C23 co-cultures in plant-based medium Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-09-11 Wei Wei Chuah, Joo Shun Tan, Siti Nur Hazwani Oslan, Pandian Bothi Raja
Abstract Lactic acid bacteria (LAB) can produce γ-aminobutyric acid (GABA) with antioxidant properties and sedative effects when it binds to the GABA receptor in the human brain. LAB can also produce bacteriocin-like inhibitory substances (BLIS) with antimicrobial capabilities during carbohydrate fermentation. GABA and BLIS are natural compounds with potential health benefits and food preservation
-
Statistical optimization of process variables for agarase production using Microbacterium sp. SS5 strain from non-marine sources Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-09-06 Dinesh Labade, Selvaraju Sevamani, Heena Tabassum, Harishkumar Madhyastha, Minal Wani
Abstract Agar oligosaccharides are thought to be valuable biomolecules with high bioactivity potential, along with a wide range of applications and advantages. The current study aimed to optimize the culture parameters required to produce agarase enzyme and agar oligosaccharides from industrial waste agar. Microbacterium spp. strain SS5 was isolated from a non-marine source and could synthesize oligo
-
Initial culture media pH influences the antibacterial activity and metabolic footprint of Lactobacillus acidophilus BIOTECH 1900 Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-09-06 Gerwin Louis T. Dela Torre, Sharon Yvette Angelina M. Villanueva
Abstract This study investigated the impact of initial culture media pH on the antibacterial properties and metabolic profile of cell-free supernatants (CFSs) from Lactobacillus acidophilus BIOTECH 1900 (LAB1900). The CFSs harvested from LAB1900 grown in de Man, Rogosa, Sharpe broth with initial pH of 5.5 (CFS5.5) and 6.6 (CFS6.6) were tested. The two CFSs elicited varying degrees of activity against
-
Aqueous two-phase extraction and characterization of thermotolerant alkaliphilic Cladophora hutchinsiae xylanase: biochemical properties and potential applications in fruit juice clarification and fish feed supplementation Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-09-05 Fulya Oz Tuncay, Ummuhan Cakmak, Yakup Kolcuoglu
Abstract Xylanase finds extensive applications in diverse biotechnological fields such as biofuel production, pulp and paper industry, baking and brewing industry, food and feed industry, and deinking of waste paper. Here, polyethylene glycol (PEG)-phosphate aqueous two-phase system (ATPS) was applied for the purification of an alkaline active and thermotolerant xylanase from a marine source, Cladophora
-
Recovery and partial purification of fibrinolytic protease from Pleurotus ostreatus and P. eryngii and cytotoxic and antioxidant activity of their extracts Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-09-05 Romário da S. Santana, Felipe de S. Mendes, Bárbara J. Paula da Silva, Emerson S. Lima, Thiago P. Nascimento, Márcia N. Carneiro da Cunha, Ana Lúcia F. Porto, Maria Francisca S. Teixeira, Rosany P. Carvalho, Waldireny R. Gomes
Abstract Mushrooms are a source of primary and secondary metabolites. Little is known about the most suitable conditions for production of mushrooms by submerged fermentation. This article reports antioxidant and cytotoxic assays, in addition to quantitatively evaluating the content of proteases with fibrinolytic action in the crude extracts of two species of edible mushrooms produced in different
-
Enhancement in T1 lipase purification recovery using the novel construct pGEX4T1/His-T1 Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-08-30 Che Haznie Ayu Che Hussian, Raja Noor Zaliha Raja Abd Rahman, Adam Thean Chor Leow, Abu Bakar Salleh, Mohd Shukuri Mohamad Ali, Wahhida Latip
Abstract The Geobacillus zalihae strain T1 produces a thermostable T1 lipase that could be used for industrial purposes. Previously, the GST-T1 lipase was purified through two chromatographic steps: affinity and ion exchange (IEX) but the recovery yield was only 33%. To improve the recovery yield to over 80%, the GST tag from the pGEX system was replaced with a poly-histidine at the N-terminal of the
-
Microbial surfactants: characteristics, production and broader application prospects in environment and industry Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-08-31 Ahmed Mohy Eldin, Nermeen Hossam
Microbial surfactants are green molecules with high surface activities having the most promising advantages over chemical surfactants including their ability to efficiently reducing surface and int...
-
Deep eutectic solvent assisted recovery of high molecular weight levan from an isolated Neobacillus pocheonensis BPSCM4 Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-08-26 P. Keerthashalini, V. Sobanadevi, Kiran Babu Uppuluri
Abstract The present study demonstrates the usage of deep eutectic solvent to recover microbial levan from the clarified fermented broth. The classic ethanol precipitation method for levan recovery is expensive because ethanol can be utilized as a biofuel. Production of ethanol consumes more energy and is not easily recycled. As a result, the current work concentrates on using environmentally friendly
-
Third generation biobutanol production by Clostridium beijerinckii in a medium containing mixotrophically cultivated Dunaliella salina biomass Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-08-23 Nazlıhan Tekin, Sevgi Ertuğrul Karatay, Gönül Dönmez
Abstract This study aims the third generation biobutanol production in P2 medium supplemented D. salina biomass mixotrophically cultivated with marble waste (MW). The wastes derived from the marble industry contain approximately 90% of carbon-rich compounds. Microalgal growth in mixotrophic conditions was optimized in the 0.4-2 g/L of MW concentration range. The highest microalgal concentration was
-
Silver and gold nanoparticles: Eco-friendly synthesis, antibiofilm, antiviral, and anticancer bioactivities Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-08-23 Waleed Elsayed Khedr, Mohamed N. F. Shaheen, Elmahdy M. Elmahdy, Magda A. El Bendary, Ahmed A. Hamed, Attiya Hamed Mohamedin
Abstract For the first time in this study, silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) were green synthesized by the cost-effective and eco-friendly procedure using Cotton seed meal and Fodder yeast extracts. The biosynthesized NPs were characterized by UV–Vis spectroscopy, dynamic light scattering analysis (DLS), transmission electron microscopy (TEM), selected area electron diffraction
-
Production of nisin from Lactococcus lactis in acid-whey with nutrient supplementation Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-08-22 Sahana Vijayakumar, Vishnu G., Prajna Rao Krishnapura, Regupathi Iyyaswami
The production of Nisin, an FDA-approved food preservative, was attempted by Lactococcus lactis subsp. lactis ATCC® 11454 using the underutilized milk industry effluent, acid-whey, as a substrate. ...
-
Purification and biochemical characterization of pullulanase produced from Bacillus sp. modified by ethyl-methyl sulfonate for improved applications Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-08-17 Oladipo O. Olaniyi, Blessing Oriade, Olusola T. Lawal, Adeyemi O. Ayodeji, Yetunde O. Olorunfemi, Festus O. Igbe
Abstract Strain improvement via chemical mutagen could impart traits with better enzyme production or improved characteristics. The present study sought to investigate the physicochemical properties of pullulanase produced from the wild Bacillus sp and the mutant. The pullulanases produced from the wild and the mutant Bacillus sp. (obtained via induction with ethyl methyl sulfonate) were purified in
-
High purity of human secreted phospholipase A2 group IIE in Pichia pastoris using basal salts medium comparison with YPD medium Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-08-14 Shulin Hou, Chunting Chen, Huili He, Haishan Yang, Ruining Li, Junping Bai, Sijin Li, Jun Xie
Abstract Secreted phospholipase A2s (sPLA2s) are a group of enzymes with 6–8 disulfide bonds that participate in numerous physiological processes by catalyzing the hydrolysis of phospholipids at the sn-2 position. Due to their high content of disulfide bonds and hydrolytic activity toward cell membranes, obtaining the protein of sPLA2s in the soluble and active form is challenging, which hampers their
-
In vitro antioxidant activity evaluation of pine nut peptides (Pinus koraiensis) fermented by Bacillus subtilis LS-45 Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-08-11 Jiajia Sun, Zhi Zhang, Kexin Yang, Gang Wei, Yanxia Li
In this study, we utilized the remarkable capabilities of Bacillus subtilis ls-45 during the fermentation process to generate pine nut peptide. Through gene sequencing, we confirmed the proficiency...
-
Effects of astaxanthin on expression of apoptosis and oxidative stress related genes in H2O2 induced oxidative stress BE(2)-C human neuroblastoma cell line Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-08-10 Maryam Hormozi, Nasim Beigi Boroujeni, Mandana Beigi Boroujeni
Abstract Antioxidants may affect the apoptosis induced by oxidative stress experimental models. The present study was conducted to investigate the effects of astaxanthin on expression of apoptosis and oxidative stress-related genes in H2O2 induced oxidative stress BE(2)-C human neuroblastoma cell line. This experimental study consisted of six groups including control, H2O2 induced oxidative stress
-
Optimizing the extraction of active components from Salvia miltiorrhiza by combination of machine learning models and intelligent optimization algorithms and its correlation analysis of antioxidant activity Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-08-10 Binhao Chen, Yali Zhao, Dingyi Yu, Feifei Lin, Zhengyuan Xu, Jingmei Song, Xiaohong Li
Abstract We extracted Sal B and TIIA from Salvia miltiorrhiza using enzymatic-assisted ethanol extraction. ACONN predicted optimal process conditions. Enzymolysis and alcohol extraction were used, optimizing conditions and evaluating antioxidant activity. ACONN analyzed data and ACO optimized conditions. Lab verification comprehensively evaluated the conditions. The correlation between Sal B, TIIA
-
Effects of astaxanthin on expression of apoptosis and oxidative stress related genes in H2O2 induced oxidative stress BE(2)-C human neuroblastoma cell line Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-08-10 Maryam Hormozi, Nasim Beigi Boroujeni, Mandana Beigi Boroujeni
Antioxidants may affect the apoptosis induced by oxidative stress experimental models. The present study was conducted to investigate the effects of astaxanthin on expression of apoptosis and oxida...
-
Purification and biochemical characterization of α- amylase from Aspergillus tamarii MTCC5152 Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-07-26 Premalatha Arunachallam, Vijayalakshmi Kumaravel, Suseela Rajakumar Gopal
The purification and biochemical characterization of the extracellular alpha amylase from A.tamarii MTCC5152 were studied. The combined use of ion exchange and gel filtration chromatographic method...
-
Novel extracellular synthesized silver nanoparticles using thermophilic Anoxybacillus flavithermus and Geobacillus stearothermophilus and their evaluation as nanodrugs Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-07-15 Gülay Giray, Serpil Gonca, Sadin Özdemir, Zelal Isik, Erkan Yılmaz, Mustafa Soylak, Nadir Dizge
Abstract In this investigation, two new thermophilic bacteria were isolated. The new isolates were characterized by 16S rRNA, biochemical, morphological, and physiological analyzes and the isolates were identified as Geobacillus stearothermophilus strain Gecek20 and thermophilic Anoxybacillus flavithermus strain Gecek19. Various biological activities of extracellular Ag-NPs synthesized from thermophilic
-
Targeting of human fibroblast growth factor receptor 2 by a novel specific nanobody Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-07-15 Mahboubeh Irani, Mahdi Habibi-Anbouhi, Mahdi Behdani, Fatemeh Kazemi-Lomedasht
Abstract Inhibition of FGFR2 signaling is promising in targeted therapy of FGFR2-related tumors. In this study, anti-FGFR2 nanobodies (Nbs) were isolated through screening of an immune camelid phage display library. Four rounds of biopanning were carried out with commercial human FGFR2 antigen and enrichment was assessed by ELISA and phage titration. The gene of Nb was sub-cloned into the expression
-
Flask and reactor scale production of plant growth regulators by Inonotus hispidus: optimization, immobilization and kinetic parameters Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-07-05 Bilal Doğan, Zeki Yıldız, Nilüfer Aksöz, Ayşe Betül Eninanç, İlknur Dağ, Abdunnasır Yıldız, Hasan Hüseyin Doğan, Mustafa Yamaç
The aims of the presented study are to compare submerged, static, and solid-state fermentation in the production of gibberellic acid (GA3), indole acetic acid (IAA), and abscisic acid (ABA) by Inon...
-
The extraction and anti-inflammatory screening of Onosma glomeratum Y. L. Liu Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-07-03 Qian Yang, Zhengyu Wang, Er-bu Aga, Xiaoxia Liang
Abstract “Zicao” has a long medicinal history and has a variety of pharmacological activities. As the main resource of “zicao” in Tibet, Onosma glomeratum Y. L. Liu (tuan hua dian zi cao), usually used for treating pneumonia in Tibet, has not been reported deeply. In order to determine the main anti-inflammatory active ingredients of Onosma glomeratum Y. L. Liu, in this study, the extracts enriched
-
Modeling and evaluation of the sucrose-degrading activity of recombinantly produced oligo-1,6-glucosidase from A. gonensis Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-06-28 Hakan Karaoglu, Zeynep Dengız Balta
Abstract Fructose is the most preferred sugar to provide benefits for sweetening and health. As many industrial enzymes are used to produce High Fructose Syrup (HFS), it is vital to explore alternative enzymes for fructose production. Oligo-α-1,6-glucosidase (O-1-6-glucosidase) hydrolyzes non-reducing ends of isomaltooligosaccharides, panose, palatinose, and an a-limit dextrin by breaking α-1,6-glucoside
-
Extracellular collagenase isolated from Streptomyces antibioticus UFPEDA 3421: purification and biochemical characterization Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-06-24 Elizianne Pereira Costa, Romero Marcos Pedrosa Brandão-Costa, Wendell Wagner Campos Albuquerque, Thiago Pajeú Nascimento, Amanda Emmanuelle Sales Conniff, Kethylen Barbara Barbosa Cardoso, Anna Gabrielly Duarte Neves, Juanize Matias da Silva Batista, Ana Lúcia Figueiredo Porto
Collagenases are proteases able to degrade native and denatured collagen, with broad applications such as leather, food, and pharmaceutical industries. The aim of this research was to purify and ch...
-
Extracellular collagenase isolated from Streptomyces antibioticus UFPEDA 3421: purification and biochemical characterization Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-06-24 Elizianne Pereira Costa, Romero Marcos Pedrosa Brandão-Costa, Wendell Wagner Campos Albuquerque, Thiago Pajeú Nascimento, Amanda Emmanuelle Sales Conniff, Kethylen Barbara Barbosa Cardoso, Anna Gabrielly Duarte Neves, Juanize Matias da Silva Batista, Ana Lúcia Figueiredo Porto
Abstract Collagenases are proteases able to degrade native and denatured collagen, with broad applications such as leather, food, and pharmaceutical industries. The aim of this research was to purify and characterize a collagenase from Streptomyces antibioticus. In the present work, the coffee ground substrate provided conditions to obtaining high collagenase activity (377.5 U/mL) using anion-exchange
-
Optimization of ultrasound- and enzymatic-assisted extractions of polyphenols from dried red onion peels and evaluation of their antioxidant activities Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-06-22 Karima S. M. Hammad, Tarek Hefzalrahman, Mohamed K. S. Morsi, Nashwa F. S. Morsy, Ekram A. Abd El-Salam
Abstract There is a recognized need for exploring new natural antioxidants that have high antioxidant potential. Thus, the aim of this study was to optimize the extraction process of onion peels using ultrasound- and enzymatic-assisted extractions (UAE and EAE) methods to utilize the optimal extracts as natural antioxidants. Box–Behnken Design (BBD) was implemented to reach the optimal extracts with
-
Factors affecting therapeutic protein purity and yield during chromatographic purification Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-05-26 Che Haznie Ayu Che Hussian, Wai Yie Leong
Abstract Therapeutic proteins are recombinant proteins generated through recombinant DNA technology and have attracted a great deal of interest in numerous applications, including pharmaceutical, cosmetic, human and animal health, agriculture, food, and bioremediation. Producing therapeutic proteins on a large scale, mainly in the pharmaceutical industry, necessitates a cost-effective, straightforward
-
High-level production and purification of bioactive recombinant human activin A in Chinese hamster ovary cells Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-05-24 Changin Kim, Hyunjoo Kim, Jeong Soo Park, Jiwon Park, Jeongmin Oh, Jaeseung Yoon, Kwanghee Baek
Abstract Activin A, a member of the TGF-β superfamily, is a homodimer of the inhibin βΑ subunit that plays a diversity of roles in biological processes. Because of its multiple functions, significant efforts have been made to produce activin A, however, unsatisfactory results were obtained due to its low level of expression. In this study, a stable CHO cell line exhibiting high expression of rhActivin
-
A review on pretreatment methods for lipid extraction from microalgae biomass Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-05-23 Muhammad Azreen Mat Husin, Nazlina Haiza Mohd Yasin, Mohd Sobri Takriff, Nur Hidayah Jamar
Abstract Microalgal lipids are promising and sustainable sources for the production of third-generation biofuels, foods, and medicines. A high lipid yield during the extraction process in microalgae could be influenced by the suitable pretreatment and lipid extraction methods. The extraction method itself could be attributed to the economic and environmental impacts on the industry. This review summarizes
-
Application of the novel manta-ray foraging algorithm to optimize acidic peptidase production in solid-state fermentation using binary agro-industrial waste Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-05-21 Maurice Ekpenyong, Atim Asitok, Ubong Ben, Andrew Amenaghawon, Heri Kusuma, Anthony Akpan, Sylvester Antai
Abstract Peptidases, which constitute about 20% of the global enzyme market, have found applications in detergent, food and pharmaceutical industries, and could be produced on a large scale using low-cost agro-industrial waste. An acidophilic Bacillus cereus strain produced acidic peptidase on binary-agro-industrial waste comprising yam peels and fish processing waste at pH 4.5 with high catalytic
-
Recovery and separation of glycyrrhizic acid from Natural Deep Eutectic Solvent (NADES) extract by macroporous resin: adsorption kinetics and isotherm studies Prep. Biochem. Biotechnol. (IF 2.9) Pub Date : 2023-05-19 Kavita J. Lanjekar, Virendra K. Rathod
Abstract Natural Deep Eutectic Solvents (NADESs) have emerged as a green and sustainable alternative to conventional organic solvents to extract bioactive compounds. However, the recovery of bioactive compounds from the NADES extracts is challenging, restricting their large-scale applications. The present work investigated the recovery of glycyrrhizic acid (GA) from choline-chloride/lactic acid NADES