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Identification the Competing Endogenous RNA Networks in Oxidative Stress Injury of Melanocytes DNA Cell Biol. (IF 3.191) Pub Date : 2021-01-20 Si Li; Hongliang Zeng; Jinhua Huang; Jianyun Lu; Jing Chen; Ying Zhou; Lan Mi; Xiaojiao Zhao; Li Lei; Qinghai Zeng
Competing endogenous RNAs (ceRNAs), including long noncoding RNA (lncRNA), circular RNA (circRNA), pseudogenes, synthetic miRNA inhibitors, etc. are classes of RNAs that can compete and interact with each other within an organism. There are regions in these RNAs that can be bound by messenger-RNA-interfering complementary RNA (microRNA), called microRNA response elements (MREs). These RNAs compete
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Cardiac Troponin I R193H Mutation Is Associated with Mitochondrial Damage in Cardiomyocytes DNA Cell Biol. (IF 3.191) Pub Date : 2021-01-18 Jing Luo; Weian Zhao; Yi Gan; Bo Pan; Lingjuan Liu; Zhenguo Liu; Jie Tian
Malfunction of myocardial mitochondria plays a crucial role in the development of cardiovascular disorders, especially hypertrophic and dilated cardiomyopathies. Cardiac troponin I (cTnI) is an important structural protein and essential to contraction and relaxation of cardiomyocytes. Recent studies suggest that mutated cTnIR193H could function as a regulatory molecule for other cell functions. This
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Unexpected Role of Nonimmune Cells: Amateur Phagocytes DNA Cell Biol. (IF 3.191) Pub Date : 2021-01-13 Maic Audo Eybi Mayer Sihombing; Maharani Safitri; Tian Zhou; Lu Wang; Sean McGinty; Hai-Jun Zhang; Yuxia Yin; Qin Peng; Juhui Qiu; Guixue Wang
Effective and efficient efferocytosis of dead cells and associated cellular debris are critical to tissue homeostasis and healing of injured tissues. This important task was previously thought to be restricted to professional phagocytes (PPs). However, accumulating evidence has revealed another type of phagocyte, the amateur phagocyte (AP), which can also participate in efferocytosis. APs are non-myeloid
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Molecular Characteristics of N1-Methyladenosine Regulators and Their Correlation with Overall Cancer Survival DNA Cell Biol. (IF 3.191) Pub Date : 2021-01-08 Juan Li; Chunting Zhang; Xin Yuan; Yuan Cao
N1-methyladenosine (m1A) is a prevalent RNA modification widely affecting RNA structural stability, folding, and interactions with proteins. Recently, there have been increasing reports on the roles of m1A regulators in tumors. However, their mechanisms and clinical relevance remain unclear. This study systematically evaluates the epigenetic characteristics and clinical relevance of m1A regulators
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A Simple and Efficient Method to Generate Gene-Knockout and Transgenic Cell Lines DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-31 Jieyu Liu; Yan Ge; Na Wang; Jing Wen; Wei Wang; Bo Zeng; Gui-Lan Chen
Knockout (KO) or exogenous expression of a gene of interest in cultured cells is one of the most important ways to study the function of the gene. Compared with transient transfection, stable cell lines possess great advantages such as excellent cell homogeneity and feasibility for long-term use. However, technical challenges in generating stable cell lines still exist in many laboratories using conventional
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Identification of Myocardial Infarction-Associated Genes Using Integrative microRNA-Gene Expression Network Analysis DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-31 Long Wang; Wei Yuan; Jinyu Huang
It is crucial to identify potential molecular targets and their interaction involved in myocardial infarction (MI). In our study, we obtained microarray data of MI from GEO database and identify differentially expressed mRNAs and microRNAs (miRNAs). Compared with normal tissues, 686 mRNAs and 16 miRNAs were differentially expressed in MI. Subsequently, function enrichment analysis was performed to
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Identification of MYCN-Related Gene as a Potential Biomarker for Neuroblastoma Prognostic Model by Integrated Analysis and Quantitative Real-Time PCR DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-30 FengLing Shao; Zhenni Wang; Shan Wang
Neuroblastoma (NB) has the highest incidence of all extracranial solid tumors in children and is highly lethal. This study aims to establish a prognostic model of NB with MYCN-related genes. We determined the gene expression profiles of 900 NB samples from the UCSC database and four Gene Expression Omnibus (GEO) data sets, and performed a comprehensive bioinformatics analysis and clinical sample verification
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Genome-Wide Identification of Key Candidate microRNAs and Target Genes Associated with Peanut Drought Tolerance DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-29 Jingyao Ren; He Zhang; Xiaolong Shi; Xin Ai; Jiale Dong; Xinhua Zhao; Chao Zhong; Chunji Jiang; Jing Wang; Haiqiu Yu
Peanut is an important crash crop worldwide, and it is often threatened by drought stress due to unexpected extreme weather events. In this work, NH5 and FH18 were selected as drought-tolerant and drought-sensitive varieties, respectively. Comparison of their physiological responses revealed that NH5 showed less wilting, higher relative water content and lower water loss rate of detached leaves, lower
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Lipid Peroxidation, GSH Depletion, and SLC7A11 Inhibition are Common Causes of EMT and Ferroptosis in A549 Cells, but Different in Specific Mechanisms DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-22 Lulu Sun; Hongliang Dong; Weiqun Zhang; Nan Wang; Na Ni; Xuelian Bai; Naiguo Liu
Epithelial-mesenchymal transition (EMT) induced by transforming growth factor-β1 (TGF-β1) is thought to be involved in the pathogenesis of pulmonary fibrosis. Emerging evidence suggested that there are some common causes between ferroptosis and pulmonary fibrosis. The interaction of EMT and ferroptosis and its mechanism were investigated by detecting the expression levels of α-smooth muscle actin (α-SMA)
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Evaluation of ICAM-1 rs5498 and rs3093030 Polymorphisms in Chinese Patients with Colorectal Cancer DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-21 Zhiyuan Qiu; Zhiqiang Xie; Rong Qin; Meifang Chen; Han He; Zhao Zhang; Yan Wang; Ming Hong; Weifeng Tang; Yan Xi; Sheng Zhang
Colorectal cancer (CRC) is a common cancer threatening human health. Intercellular adhesion molecule-1 (ICAM-1, CD54) displays a key role in carcinogenesis and previous studies have suggested that ICAM-1 single-nucleotide polymorphisms (SNPs) are predicted to increase the risk of CRC. However, the relationship of ICAM-1 SNPs with CRC susceptibility was controversial. We conducted a case–control study
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Identification and Validation of Key miRNAs and a MicroRNA-mRNA Regulatory Network Associated with Ulcerative Colitis DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-21 Shanshan Wang; Lei Shen; Hesheng Luo
Ulcerative colitis (UC) is a chronic, nonspecific, intestinal inflammatory disease that involves various genes and pathways in its pathogenesis. The current study revealed the key miRNAs and potential target gene regulatory network as a model for predicting the molecular mechanism of UC. This may provide novel insights for unraveling the pathogenesis of UC. Differentially expressed miRNAs (DEMIs) and
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Parp1-Dependent DNA Double-Strand Break Repair in Irradiated Late Pachytene Spermatocytes DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-18 Emad A. Ahmed; Abdullah M. Alzahrani; Harry Scherthan
Poly (ADP-ribose) polymerase-1 (Parp1) is a member of nuclear enzymes family involved in to the response to genotoxic stresses, DNA repair, and is critical for the maintenance of genome stability. During gametogenesis, genome stability is essential for inheritance and formation of healthy gametes. The latter involves DNA double-strand break (DSB)-driven pairing of homologous chromosomes in first meiotic
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Transcription Factors ZEB1 and CREB Promote the Transcription of Bovine ABHD5 Gene DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-17 Xiaoyu Wang; Anning Li; Sayed Haidar Abbas Raza; Chengcheng Liang; Song Zhang; Chugang Mei; Wucai Yang; Linsen Zan
Alpha/beta hydrolase domain 5 (ABHD5) plays a significant role in intracellular lipid metabolism, which is regulated by a complex network of transcription factors. The transcriptional regulation of the ABHD5 gene in cattle and other livestock, however, has not been previously investigated. Investigations in humans and animal models indicate that the transcription factors zinc finger E-box binding homeobox
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Long Noncoding RNAs in the Progression of Atherosclerosis: An Integrated Analysis Based on Competing Endogenous RNA Theory DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-17 Cheng Qian; Meng Xia; Xueying Yang; Pengfei Chen; Qiang Ye
Long noncoding RNAs (lncRNAs) have been increasingly accepted to function importantly in human diseases by serving as competing endogenous RNAs (ceRNAs). To date, the ceRNA mechanisms of lncRNAs in the progression of atherosclerosis (AS) remain largely unclear. On the basis of ceRNA theory, we implemented a multistep computational analysis to construct an lncRNA–mRNA network for AS progression (ASpLMN)
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Nucleotide Sequence Variation in the Insulin-Like Growth Factor 1 Gene Affects Growth and Carcass Traits in New Zealand Romney Sheep DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-16 Shaobin Li; Huitong Zhou; Fangfang Zhao; Qian Fang; Jiqing Wang; Xiu Liu; Yuzhu Luo; Jon G.H. Hickford
Insulin-like growth factor 1 (IGF1) is a mediator of the effects of growth hormone and polymorphism in the IGF1 gene (IGF1) is reported to affect fat deposition in some livestock species. In this study, nucleotide sequence variation in three regions of ovine IGF1 (part of the 5′ flanking region, the exon 3 region, and the exon 4 region) was investigated in 848 New Zealand Romney lambs using PCR-single
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R-Spondins 2 and 3 Are Overexpressed in a Subset of Human Colon and Breast Cancers DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-15 Caitlin B. Conboy; Germán L. Vélez-Reyes; Susan K. Rathe; Juan E. Abrahante; Nuri A. Temiz; Michael B. Burns; Reuben S. Harris; Timothy K. Starr; David A. Largaespada
Wnt signaling is activated in many cancer types, yet targeting the canonical Wnt pathway has been challenging for cancer therapy. The pathway might be effectively targeted at many levels depending on the mechanism by which it has become hyperactive. Recently, mouse genetic screens have found that R-spondins (RSPOs) act as oncogenes. Evidence includes recurrent genomic rearrangements that led to increased
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S100A2 Silencing Relieves Epithelial–Mesenchymal Transition in Pulmonary Fibrosis by Inhibiting the Wnt/β-Catenin Signaling Pathway DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-11 Guichuan Huang; Jing Zhang; Gang Qing; Daishun Liu; Xin Wang; Yi Chen; Yishi Li; Shuliang Guo
Pulmonary fibrosis (PF) is a progressive and lethal disease with poor prognosis. S100A2 plays an important role in the progression of cancer. However, the role of S100A2 in PF has not yet been reported. In this study, we explored the potential role of S100A2 in PF and its potential molecular mechanisms. Increased expression of S100A2 was first observed in lung tissues of PF patients. We found that
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Brain Transcriptome Analysis Reveals Potential Transcription Factors and Biological Pathways Associated with Feed Efficiency in Commercial DLY Pigs DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-09 Cineng Xu; Xingwang Wang; Shenping Zhou; Jie Wu; Qian Geng; Donglin Ruan; Yibin Qiu; Jianping Quan; Rongrong Ding; Gengyuan Cai; Zhenfang Wu; Enqin Zheng; Jie Yang
Feed efficiency (FE) is one of the most important economic traits in the porcine industry. In this study, high-throughput RNA sequencing (RNA-seq) was first utilized for brain tissue transcriptome analysis in pigs to indicate the potential genes and biological pathways related to FE in pigs. A total of 8 pigs with either extremely high-FE group (HE-group) or low-FE group (LE-group) were selected from
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Genetic Polymorphisms of Delta-Like 1 Homolog (DLK1) Influence the Susceptibility to Antituberculosis Drug-Induced Hepatotoxicity DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-09 Yang Fu; Tangyuheng Liu; Jiajia Song; Lin Jiao; Juan Zhou; Hao Bai; Zhenzhen Zhao; Hao Chen; Tao Wu; Mengyuan Lyv; Yi Zhou; Binwu Ying; Yanming Xu
This study was designed to investigate the relationship between Delta-like 1 homolog (DLK1) polymorphisms and the occurrence of antituberculosis drug-induced hepatotoxicity (ATDH) in the Western Chinese Han population. A total of 746 tuberculosis patients including 118 ATDH cases and 628 non-ATDH cases were enrolled from West China Hospital of Sichuan University during 2016–2018. Ten single nucleotide
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microRNA as Biomarker in Ovarian Cancer Management: Advantages and Challenges DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-03 Vivek Kumar; Sameer Gupta; Kachnar Varma; Manisha Sachan
Ovarian cancer is the most prevalent gynecological malignancy affecting women throughout the globe. Ovarian cancer has several subtypes, including epithelial ovarian cancer (EOC) with a whopping incidence rate of 239,000 per year, making it the sixth most common gynecological malignancy worldwide. Despite advancement of detection and therapeutics, death rate accounts for 152,000 per annum. Several
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miR-2382-5p Regulates Lipid Metabolism by Targeting NDRG2 in Mammary Epithelial Cells of Dairy Cattle DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-03 Lixin Xia; Zhihui Zhao; Runjun Yang; Ping Jiang; Yinuo Liu; Haibin Yu; Zitong Bai; Jiaqi Mi; Xianzhong Yu; Xibi Fang
microRNA is a class of single-stranded RNA molecules of about 22–24 nucleotides in length, which regulate a variety of biological processes, including lipid metabolism and triglyceride synthesis at transcriptional and translational levels by degrading target mRNAs or interfering with the protein production. In this study, the effect of miR-2382-5p on triglyceride levels was examined in bovine mammary
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Probing the Strength and Mechanism of Binding Between Amifampridine and Calf Thymus DNA DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-03 Samira Sharifinia; Farshid Hajibabaei; Sadegh Salehzadeh; Neda Hosseinpour Moghadam; Sadegh Khazalpour
In this work, we have investigated the strength and mechanism of amifampridine (3,4-Diaminopyridine/3,4-DAP) interaction with calf thymus DNA (ct-DNA). The existence and the strength of interaction are evaluated using circular dichroism (CD), UV–vis absorption, and differential pulse voltammogram studies. Results from UV–vis absorption technique indicate that amifampridine can significantly interact
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Differential Expression of microRNA Profiles and Wnt Signals in Stem Cell-Derived Exosomes During Dopaminergic Neuron Differentiation DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-03 Tao Jin; Jiachen Gu; Hongbo Xia; Huimin Chen; Xiaomin Xu; Zongshan Li; Yumei Yue; Yaxing Gui
The role of secreted exosomes during dopaminergic (DA) neuron differentiation is still unknown. To investigate the roles of exosomes in DA neuron fate specification, we profiled exosomal microRNAs (miRNAs) during DA neuron differentiation of epiblast-derived stem cells (EpiSCs). There were 26 miRNAs differentially expressed (relative fold >2, p < 0.05) in EpiSC-derived exosomes at 0, 2, 4, 6, 8, 10
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BTX-A Promotes Expression of Angiogenesis-Associated Genes in Human Umbilical Vein Endothelial Cells DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-03 Xia Wu; Yu Zhang; Luxia Chen; Yongmei Han; Yinjing Song; Hao Cheng
Raynaud's phenomenon (RP) is an episodic vasospasm of the peripheral arteries caused by an exaggerated reaction to cold temperature or emotional stress. Restoring the angiogenesis capability of the acral lesional skin is a critical strategy to treat RP. Local injection of botulinum toxin-A (BTX-A) has also been reported for treatment of RP. However, since the exact mechanisms of BTX-A action are still
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lncRNA HOTAIR Promotes DNA Repair and Radioresistance of Breast Cancer via EZH2 DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-03 Liyu Qian; Qiaoman Fei; Haoran Zhang; Manman Qiu; Bo Zhang; Qin Wang; Yang Yu; Chenxu Guo; Yu Ren; Mei Mei; Ling Zhang; Yu Zhu; Bing Yang
Mounting evidence shows that long noncoding RNAs play important roles in human diseases and radioresistance could be an important factor for tumor recurrence. We observed that HOX antisense intergenic RNA (HOTAIR) expression increased in invasive ductal carcinoma (IDC) tissue. The irradiation effect of HOTAIR was detected by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium
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Identification of a Novel Six-Long Noncoding RNA Signature for Molecular Diagnosis of Dilated Cardiomyopathy DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-03 Hui Zhang; Xiajing Chen; Danfeng Zhang; Lingjuan Liu; Jing Song; Yinyin Xu; Jie Tian
Long noncoding RNAs (lncRNAs) may serve as potential molecular diagnostic markers to improve the capacity of earlier and more accurate diagnosis of dilated cardiomyopathy (DCM). We integrated five independent transcriptomic datasets (n = 504) from Gene Expression Omnibus for systematic identification of lncRNA-based diagnostic biomarkers in DCM. The multivariate logistic regression model based on the
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M6A Demethylase FTO Plays a Tumor Suppressor Role in Thyroid Cancer DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-03 Run Tian; Shidong Zhang; Daxin Sun; Chunhua Bei; Di Li; Chuanjun Zheng; Xin Song; Mengshi Chen; Shengkui Tan; Xiaonian Zhu; Huixia Zhang
The purpose of this study was to investigate the expression and clinical significance of N6-methyladenosine demethylase FTO in thyroid cancer. Bioinformatic analysis showed that FTO expression was downregulated in thyroid cancer tissues and correlated with lymph node metastasis in thyroid cancer patients. We conducted experimental verification by collecting Asian samples. The results of quantitative
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Expression Profile Analysis of m6A RNA Methylation Regulators Indicates They Are Immune Signature Associated and Can Predict Survival in Kidney Renal Cell Carcinoma DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-03 Jiuyuan Fang; Mingyang Hu; Yan Sun; Sijie Zhou; Huixiang Li
N6-Methyladenosine (m6A) refers to the methylation modification occurring at the nitrogen-6 position of adenosine. Many human physiological processes such as modulation of spermatogenesis are caused by m6A RNA modifications. However, the relationship between m6A RNA methylation regulators and kidney renal clear cell carcinoma (KIRC) remains rarely investigated. This work aimed to explore the influence
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WRKY Transcription Factors in Medicago sativa L.: Genome-Wide Identification and Expression Analysis Under Abiotic Stress DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-03 Pei Mao; Xiaoyu Jin; Qinyan Bao; Cuo Mei; Qiang Zhou; Xueyang Min; Zhipeng Liu
Alfalfa (Medicago sativa L.) is the most widely cultivated leguminous herb in the world. Its agricultural development has been restricted by various adverse environmental conditions, including water deficiency, high salinity, and low temperature. WRKY transcription factors (TFs) serve important roles in the regulation of plant development and stress responses. Research on the WRKY gene family has been
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Analysis of CNOT Family Gene Expression, Clinicopathological Features, and Prognosis Value in Hepatocellular Carcinoma DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-03 Haichao Zhao; Changzhou Chen; Xidong Chen; Donglin Zhang; Jian Li; Chuanli Yang; Chongren Ren; Xiaojing Ren; Xifeng Fu; Yanjun Li; Jiefeng He; Haoliang Zhao
The carbon catabolite repressor 4-negative on TATA (CCR4-NOT) complex, abbreviated CNOT, has deadenylation and 3′-5′ exonuclease activity, mediates deadenylation in the degradation of RNA, initiates the exonuclease degradation pathway, and participates in tumor gene regulation. CNOT proteins comprise a family of global transcriptional regulators that are evolutionarily conserved in eukaryotic cells
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Identification of Diagnostic Biomarkers of Osteoarthritis Based on Multi-Chip Integrated Analysis and Machine Learning DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-03 Yueqi Zhang; Yi Yang; Chenzhong Wang; Shengcheng Wan; Zhenjun Yao; Ying Zhang; Jinyu Liu; Chi Zhang
The pathogenesis of osteoarthritis (OA) is still unclear. It is therefore important to identify relevant diagnostic marker genes for OA. We performed an integrated analysis with multiple microarray data cohorts to identify potential transcriptome markers of OA development. Further, to identify OA diagnostic markers, we established gene regulatory networks based on the protein–protein interaction network
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miR-624-3p Promotes Esophageal Squamous Cell Carcinoma Progression via Targeting Phosphatase and Tensin Homologue DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-03 Hongyu Jia; Henan Wang; Fengyu Xia; Yan Sun; Hongli Liu; Lili Yan; Shanshan Li; Dongchun Jiang; Meimei Xu
The emerging role of miRNA as regulators in esophageal squamous cell carcinoma (ESCC) progression has aroused great attention recently. In this study, the effects of miR-624-3p in ESCC progression were explored through cell proliferation, colony formation, cell cycle, and apoptosis analyses. Results showed that increased expression of miR-624-3p enhanced cancer cell viability, proliferation, migration
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Expression and Clinical Significance of CMTM6 in Nonsmall Cell Lung Cancer DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-03 Xueyan Hou; Sisi He; Daquan Zhang; Chengli Yang; Yongli Shi; Kuanlin Zhang
Chemokine-like factor (CKLF)-like MARVEL transmembrane domain containing protein 6 (CMTM6) is a ubiquitously expressed protein, which plays a critical role in the stability of programmed death-ligand 1. However, the expression of CMTM6 in a variety of cancer pathological tissues is not clear. Therefore, 109 patients who were diagnosed with nonsmall cell lung cancer (NSCLC) and underwent surgical resection
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A Landscape of lncRNA Expression Profile and the Predictive Value of a Candidate lncRNA for Silica-Induced Pulmonary Fibrosis DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-03 Xiaohong Lei; Ailing Qing; Xuemei Yuan; Delu Qiu; Haiyu Li
Silicosis is the most common type of pneumoconiosis with the fastest progress and the most serious harm. At present, there is still a lack of effective treatment for silicosis, and the molecular mechanism of silicosis is very complex, which is not completely clear. This study aimed to identify crucial long noncoding RNA (lncRNA)–mRNA networks for silica-induced pulmonary fibrosis using microarray data
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Coptisine Blocks Secretion of Exosomal circCCT3 from Cancer-Associated Fibroblasts to Reprogram Glucose Metabolism in Hepatocellular Carcinoma DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-03 Baowei Lv; Wenyan Zhu; Chunqing Feng
Coptisine, extracted from rhizoma coptidis, has been shown to inhibit a variety of cancers. However, the underlying mechanism by which coptisine regulates hepatocellular carcinoma (HCC) progression remains unknown. MTT assay, transwell invasion assay, and TUNEL assay were employed to examine cell viability, invasion, and apoptosis. In vivo tumor growth was determined by xenograft experiment. Reverse
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Skeletal Muscle Anti-Atrophic Effects of Leucine Involve Myostatin Inhibition DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-03 André Cruz; Andrea Ferian; Paula K.N. Alves; William Jose Silva; Mirella Ribeiro Bento; Alexander Gasch; Siegfried Labeit; Anselmo Sigari Moriscot
Lack of mechanical load leads to skeletal muscle atrophy, and one major underlying mechanism involves the myostatin pathway that negatively regulates protein synthesis and also activates Atrogin-1/MAFbx and MuRF1 genes. In hindlimb immobilization, leucine was observed to attenuate the upregulation of the referred atrogenes, thereby shortening the impact on fiber cross-sectional area, nonetheless, the
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ADAMTS8 Inhibits Progression of Esophageal Squamous Cell Carcinoma DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-03 Zhonglin Wu; Yanjun Shi; Shuguang Ren; Yingchao Ju; Yueyang Hu; Jianhua Wu
A disintegrin and metallopeptidase with thrombospondin motifs (ADAMTSs), which is frequently dysregulated in cancers and is involved in carcinogenesis and cancer progression. The present study identified that ADAMTS8 expression is downregulated in esophageal squamous cell carcinoma (ESCC) tissues when compared with nontumor tissue. The expression of ADAMTS8 is closely associated with clinical stage
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Identification of SARS-CoV-2 Proteins Binding Human mRNAs As a Novel Signature Predicting Overall Survival in Hepatocellular Carcinoma DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-04 Shimin Chen
The impact of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is the virus causing coronavirus disease 2019 (COVID-19), has been confirmed in cancers through binding specific mRNAs to invade human cells. Therefore, the aim of this study described here was to develop and validate novel SARS-CoV-2 proteins binding human mRNAs (SPBRs) signature to predict overall survival (OS) in hepatocellular
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A Competing Endogenous RNA Network and an 8-lncRNA Prognostic Signature Identify MYO16-AS1 as an Oncogenic lncRNA in Bladder Cancer DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-02 Danyang Shen; Youyun Zhang; Qiming Zheng; Shicheng Yu; Liqun Xia; Sheng Cheng; Gonghui Li
Recently, growing evidence has shed light on the competitive endogenous RNAs (ceRNAs) activity of long noncoding RNAs (lncRNAs) in carcinogenesis and tumor progression. To better elucidate the regulatory mechanisms of lncRNA in muscle-invasive bladder cancer (MIBC), we identified aberrantly expressed mRNAs, lncRNAs, and miRNAs in tumor tissues by using RNA sequence profiles from The Cancer Genome Atlas
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Interaction Between microRNA and DNA Methylation in Atherosclerosis DNA Cell Biol. (IF 3.191) Pub Date : 2020-12-01 Jun Tao; Linzhen Xia; Zemin Cai; Lingli Liang; Yanjun Chen; Jun Meng; Zuo Wang
Atherosclerosis (AS) is a chronic inflammatory disease accompanied by complex pathological changes, such as endothelial dysfunction, foam cell formation, and vascular smooth muscle cell proliferation. Many approaches, including regulating AS-related gene expression in the transcriptional or post-transcriptional level, contribute to alleviating AS development. The DNA methylation is a crucial epigenetic
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Expression and Regulation of Aquaporins in Pregnancy Complications and Reproductive Dysfunctions DNA Cell Biol. (IF 3.191) Pub Date : 2020-11-23 Hailing Shao; Shichu Gao; Xinxin Ying; Xueqiong Zhu; Ying Hua
Aquaporins (AQPs), small hydrophobic integral membrane proteins, mediate rapid transport of water and small solutes. The abnormal expressions of AQPs are associated with pregnancy complications and reproductive dysfunctions, including preeclampsia, gestational diabetes mellitus, tubal ectopic pregnancy, intrahepatic cholestasis of pregnancy, preterm birth, chorioamnionitis, polyhydramnios, and oligohydramnios
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Genetic Polymorphisms of PRNCR1 and Lung Cancer Risk in Chinese Northeast Population: A Case–Control Study and Meta-Analysis DNA Cell Biol. (IF 3.191) Pub Date : 2020-11-23 Na Li; Zhigang Cui; Min Gao; Sixuan Li; Mingyang Song; Ying Wang; Lianwei Tong; Yanhong Bi; Ziwei Zhang; Shengli Wang; Baosen Zhou; Zhihua Yin
Long noncoding RNAs (lncRNAs) play vital roles in development and progression of various cancers. To investigate the relationship between three tag single-nucleotide polymorphisms (SNPs) (rs13252298, rs1016343, and rs1456315) in lncRNA prostate cancer-associated noncoding RNA 1 (PRNCR1) and lung cancer (LC) risk, we conducted this study. First, we performed a case–control study, including 576 LC patients
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Identification and Bioinformatic Assessment of circRNA Expression After RMI1 Knockdown and Ionizing Radiation Exposure DNA Cell Biol. (IF 3.191) Pub Date : 2020-11-17 Yuxiao Sun; Lianying Fang; Mengmeng Yang; Ningning He; Jinhan Wang; Manman Zhang; Kaihua Ji; Qin Wang; Yang Liu; Liqing Du; Yan Wang; Chang Xu; Qiang Liu
RecQ-mediated genome instability protein 1 (RMI1) is an important component of the BLM-Topo IIIα-RMI1-RMI2 complex and plays a critical role in maintaining genome stability. However, the cellular functions of RMI1 in response to ionizing radiation (IR) are poorly understood. In this study, we found that RMI1 knockdown led to enhanced radiosensitivity and apoptosis after irradiation. To analyze the
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Circular RNA and Messenger RNA Expression Profile and Competing Endogenous RNA Network in Subchondral Bone in Osteonecrosis of the Femoral Head DNA Cell Biol. (IF 3.191) Pub Date : 2020-11-13 Ming Jiao; Run Tian; Guanzhi Liu; Xiaohui Liu; Qilu Wei; Junteng Yan; Kunzheng Wang; Pei Yang
Osteonecrosis of the femoral head (ONFH) is a common and destructive orthopedic disease, of which the pathogenesis mechanism remains elusive. Limited studies have been conducted to investigate the role of circular RNAs (circRNAs) in subchondral bone in ONFH. This study aimed to profile differential expression of circRNAs and messenger RNAs (mRNAs) in subchondral bone obtained from ONFH patients by
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MSH6 Aggravates the Hypoxic Microenvironment via Regulating HIF1A to Promote the Metastasis of Glioblastoma Multiforme DNA Cell Biol. (IF 3.191) Pub Date : 2020-11-12 Ying Shi; Jian Jiang; Yingzhe Cui; Yaodong Chen; Tianxiu Dong; Hongda An; Pengfei Liu
Glioblastoma multiforme (GBM) is characterized by diffuse infiltration of the brain, active regional recurrence, low cure proportion, and limited chemotherapy efficiency. MutS homolog 6 (MSH6) is a component of the mismatch repair system related to the oncogenesis, tumor evolution, and recurrence of GBM. The impact of MSH6 upregulation on the tumor microenvironment (TME) of GBM and the feasibility
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Identification of a Missense Mutation in the Surfactant Protein A2 Gene in a Chinese Family with Interstitial Lung Disease DNA Cell Biol. (IF 3.191) Pub Date : 2020-11-12 Lv Liu; Yi-Jie Liu; Ting Guo; Hong Luo
Interstitial lung disease (ILD) is a large group of disorders, most of which lead to progressive scarring of lung tissue. The scarring associated with ILD eventually affects your ability to breathe and get enough oxygen into your bloodstream. The typical symptoms of ILD are shortness of breath at rest or aggravated by exertion and dry cough. In this study, we enrolled a family with ILDs from central
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Increased Expression of UBE2T Predicting Poor Survival of Epithelial Ovarian Cancer: Based on Comprehensive Analysis of UBE2s, Clinical Samples, and the GEO Database DNA Cell Biol. (IF 3.191) Pub Date : 2020-11-11 Ruoyao Zou; Haoya Xu; Feifei Li; Shengke Wang; Liancheng Zhu
Ubiquitin-conjugating enzymes E2 (UBE2) have been reported in the microenvironment of various malignant tumors, but their correlation with ovarian cancer (OC) remains elusive. This study aimed to systematically analyze the expression patterns, prognostic value, genetic variation, and biological functions of 12 members of the UBE2 gene family in OC through the Oncomine, Gene Expression Profiling Interactive
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A Novel COX10 Deletion Polymorphism as a Susceptibility Factor for Sudden Cardiac Death Risk in Chinese Populations DNA Cell Biol. (IF 3.191) Pub Date : 2020-11-11 Zhenzhen Yang; Qing Zhang; Huan Yu; Lijuan Li; Yan He; Shaohua Zhu; Chengtao Li; Suhua Zhang; Bin Luo; Yuzhen Gao
Identifying common genetic variations that are related to sudden cardiac death (SCD) is crucial since it can facilitate the diagnosis and risk stratification of SCD. It has been reported that COX10 mutations might be related with SCD. In this study, we performed a systematic variant screening on the COX10 to filter potential functional genetic variations. Based on the screening results, an insertion/deletion
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Harmine Combined with Rad54 Knockdown Inhibits the Viability of Echinococcus granulosus by Enhancing DNA Damage DNA Cell Biol. (IF 3.191) Pub Date : 2020-11-10 Yuehong Gong; Chunyan Tian; Shuai Lu; Yi Gao; Limei Wen; Bei Chen; Huijing Gao; Haibo Zhang; Jun Zhao; Jianhua Wang
This study aimed at exploring the role of EgRad54 and the effect of harmine (HM) or HM derivatives (HMDs) on DNA damage in Echinococcus granulosus. DNA damage in E. granulosus protoscoleces (PSCs) was assessed by using a comet assay, after treatment with HM or HMDs. Efficiency of electroporation-based transfection of PSCs and subsequent EgRad54 knockdown was evaluated by using real-time quantitative
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R-Loop Physiology and Pathology: A Brief Review DNA Cell Biol. (IF 3.191) Pub Date : 2020-11-04 Ryan Patrick Mackay; Qinqin Xu; Paul M. Weinberger
Physiological and pathological roles for R-loop structures continue to be discovered, and studies suggest that R-loops could contribute to human disease. R-loops are nucleic acid structures characterized by a DNA:RNA hybrid and displaced single-stranded DNA that occur in connection with transcription. R-loops form naturally and have been shown to be important for a number of physiological processes
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20 Years an Orphan: Is GPR84 a Plausible Medium-Chain Fatty Acid-Sensing Receptor? DNA Cell Biol. (IF 3.191) Pub Date : 2020-11-04 Vincent B. Luscombe; Daniel Lucy; Carole J.R. Bataille; Angela J. Russell; David R. Greaves
GPR84 is an inflammation-induced receptor highly expressed on immune cells, yet its endogenous ligand is still unknown. This makes any interpretation of its physiological activity in vivo difficult. However, experiments with potent synthetic agonists have highlighted what the receptor can do, namely, enhance proinflammatory signaling and macrophage effector functions such as phagocytosis. Developing
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New Insight of Circular RNAs in Human Musculoskeletal Diseases DNA Cell Biol. (IF 3.191) Pub Date : 2020-11-04 Yuzhe Liu; Xinming Gu; He Liu; Zhaoyan Li; Zhonghan Wang; Zhengqing Zhu; Weinan Gao; Jincheng Wang
Circular RNAs (circRNAs), a novel group of noncoding RNAs, are present in most eukaryotic cells. Different from messenger RNAs, circRNAs have a covalently closed single-stranded stable structure and often act in cell type and tissue-specific manners, indicating that they can be used as biomarkers. With the advance of high-throughput RNA sequencing technology and bioinformatics, a large number of circRNAs
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MAP30 Inhibits Bladder Cancer Cell Migration and Invasion In Vitro Through Suppressing Akt Pathway and the Epithelial/Mesenchymal Transition Process DNA Cell Biol. (IF 3.191) Pub Date : 2020-11-04 Shaoqi Zhang; Zhenduo Shi; Kun Pang; Houguang He; Jiangang Chen; Zhiguo Zhang; Qianjin Zhang; Lin Hao; Conghui Han
The antitumor activity of Momordica anti-human immunodeficiency virus protein of 30 kDa (MAP30) has been proved. However, the role of MAP30 on tumor metastasis has not yet been identified. For this purpose, we investigated this effect and underlying mechanism of MAP30 in bladder cancer (BC). Here, we reported that MAP30 significantly inhibited the cell proliferation and clone formation of 5637 and
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Aberrantly Methylated and Expressed Genes as Prognostic Epigenetic Biomarkers for Colon Cancer DNA Cell Biol. (IF 3.191) Pub Date : 2020-11-04 Yuanyu Wu; Xiaoyu Wan; Guoliang Jia; Zhonghang Xu; Youmao Tao; Zheyu Song; Tonghua Du
This study aimed to identify prognostic epigenetic biomarkers for colon cancer (CC). Methylation and mRNA expression in CC samples with clinical characteristics that corresponded to those in The Cancer Genome Atlas were analyzed. Differentially methylated genes (DMGs) and differentially expressed genes (DEGs) were screened between matched tumor and nontumor tissues. Among the 415 DEGs and DMGs that
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The Polymorphism rs2968 of LSS Gene Confers Susceptibility to Age-Related Cataract. DNA Cell Biol. (IF 3.191) Pub Date : 2020-11-04 Xi Zou,Hao Wang,Dong Zhou,Zhinan Liu,Yong Wang,Guohua Deng,Huaijin Guan
Research showed that lanosterol can decrease protein aggregation in lens and reduce cataract formation. Lanosterol synthase (LSS) and 3-Hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) are the limiting enzymes in the process of synthesis of lanosterol. We demonstrate to investigate the association between functional single-nucleotide polymorphisms (SNPs) of LSS and HMGCR genes and age-related
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Genome-Wide Identification and Expression Analysis of the Dof Gene Family in Medicago sativa L. Under Various Abiotic Stresses DNA Cell Biol. (IF 3.191) Pub Date : 2020-11-04 Bo Cao; Yue Cui; Keke Lou; Dong Luo; Zhipeng Liu; Qiang Zhou
The Dof transcription factor is a plant-specific transcriptional regulator that plays important roles in plant development and acts as a mediator in plant external stress responses. However, Dofs have previously been identified in several plants but not in alfalfa (Medicago sativa L.), one of the most widely cultivated forage legumes. In the present study, a total of 40 MsDof genes were identified
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Genome-Wide Analysis of the Heat Shock Transcription Factor Gene Family in Brassica juncea: Structure, Evolution, and Expression Profiles. DNA Cell Biol. (IF 3.191) Pub Date : 2020-11-04 Mengyao Li,Fangjie Xie,Yanwen Li,Li Gong,Ya Luo,Yong Zhang,Qing Chen,Yan Wang,Yuanxiu Lin,Yunting Zhang,Xiaorong Wang,Haoru Tang
Heat shock transcription factor (HSF) is ubiquitous in the whole biological world and plays an important role in regulating growth and development and responses to environment stress. In this study, a total of 60 HSF transcription factors in Brassica juncea genome were identified and analyzed. Phylogenetic analysis showed that HSF genes were divided into three groups namely: A, B, and C, of which group
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Differential mRNA and Long Noncoding RNA Expression Profiles in Umbilical Cord Blood Exosomes from Gestational Diabetes Mellitus Patients DNA Cell Biol. (IF 3.191) Pub Date : 2020-11-04 Minkai Cao; Le Zhang; Yu Lin; Zhengying Li; Jianjuan Xu; Zhonghua Shi; Zhong Chen; Jinqi Ma; Juan Wen
Background and Aims: Exosomes contain numerous RNAs and transfer them between cells or organs, thereby establishing intercellular or interorgan communication. The roles of mRNAs and long noncoding RNAs (lncRNAs) from umbilical cord blood exosomes in gestational diabetes mellitus (GDM) occurrence and fetus growth remain poorly understood. We aimed to establish the differential mRNA and lncRNA expression
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miR-608 rs4919510 Polymorphism May Affect Susceptibility to Colorectal Cancer by Upregulating MRPL43 Expression DNA Cell Biol. (IF 3.191) Pub Date : 2020-11-04 Xiaoqi Zhu; Yichen Liu; Jingsheng Xu; Zhounan Cheng; Yuhui Yu; Minjie Chu; Xiao Lu; Weiyan Yuan
There are many studies on the association between miR-608 rs4919510 polymorphism and susceptibility to colorectal cancer (CRC). However, the role of rs4919510 in CRC development and its underlying mechanism remain unclear. We first evaluated the gene that may be regulated by the variation of rs4919510 through a two-stage expression quantitative trait loci analysis and then compared the expression of