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Genetic Predisposition for White Matter Hyperintensities and Risk of Mild Cognitive Impairment and Alzheimer's Disease: Results from the HELIAD Study. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-22 Stefanos N Sampatakakis, Niki Mourtzi, Sokratis Charisis, Eirini Mamalaki, Eva Ntanasi, Alexandros Hatzimanolis, Alfredo Ramirez, Jean-Charles Lambert, Mary Yannakoulia, Mary H Kosmidis, Efthimios Dardiotis, Georgios Hadjigeorgiou, Paraskevi Sakka, Nikolaos Scarmeas
The present study investigated the association of genetic predisposition for white matter hyperintensities (WMHs) with incident amnestic mild cognitive impairment (aMCI) or Alzheimer's disease (AD), as well as whether such an association was influenced by age, sex, and cognitive reserve. Overall, 537 individuals without aMCI or dementia at baseline were included. Among them, 62 individuals developed
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Ghrelin in Focus: Dissecting Its Critical Roles in Gastrointestinal Pathologies and Therapies. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-22 Wei Wu, Lei Zhu, Zhimin Dou, Qiliang Hou, Sen Wang, Ziqian Yuan, Bin Li
This review elucidates the critical role of ghrelin, a peptide hormone mainly synthesized in the stomach in various gastrointestinal (GI) diseases. Ghrelin participates in diverse biological functions ranging from appetite regulation to impacting autophagy and apoptosis. In sepsis, it reduces intestinal barrier damage by inhibiting inflammatory responses, enhancing GI blood flow, and modulating cellular
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Evaluation of the Reparative Effect of Sinomenine in an Acetaminophen-Induced Liver Injury Model. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-21 Ahmet Kayalı, Ejder Saylav Bora, Hüseyin Acar, Oytun Erbaş
Due to its rising global prevalence, liver failure treatments are urgently needed. Sinomenine (SIN), an alkaloid from sinomenium acutum, is being studied for its liver-repair properties due to Acetaminophen (APAP) overdose. SIN's effect on APAP-induced hepatotoxicity in rats was examined histologically and biochemically. Three groups of 30 adult male Wistar rats were created: control, APAP-only, and
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Coffee and Microbiota: A Narrative Review. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-20 Federico Rosa, Benedetta Marigliano, Sergio Mannucci, Marcello Candelli, Gabriele Savioli, Giuseppe Merra, Maurizio Gabrielli, Antonio Gasbarrini, Francesco Franceschi, Andrea Piccioni
Coffee is one of the most widely consumed beverages in the world, which has important repercussions on the health of the individual, mainly because of certain compounds it contains. Coffee consumption exerts significant influences on the entire body, including the gastrointestinal tract, where a central role is played by the gut microbiota. Dysbiosis in the gut microbiota is implicated in the occurrence
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In Vitro Anti-Inflammatory Study of Limonoids Isolated from Chisocheton Plants. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-20 Erina Hilmayanti, Xuhao Huang, Supriatno Salam, Nurlelasari, Unang Supratman, Kazuya Kabayama, Koichi Fukase
Chisocheton plants from the family Meliaceae have traditionally been used to treat several diseases; however, scientific evidence is limited. The most abundant chemical constituents of this plant are the limonoids, which are known for their various biological activities, including anti-inflammatory effects. However, the anti-inflammatory effects and underlying mechanisms of action of the constituents
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Inflammation and Starvation Affect Housekeeping Gene Stability in Adipose Mesenchymal Stromal Cells. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-19 Enrico Ragni, Simona Piccolo, Michela Taiana, Caterina Visconte, Giulio Grieco, Laura de Girolamo
Due to the scientific success of in vitro and in vivo model studies, the interest in using mesenchymal stromal cells (MSCs) for the treatment of orthopaedic conditions is growing. In the context of osteoarthritis (OA), MSCs, and, in particular, those derived from adipose tissues (ASCs), have found broader access to clinical use as active components of minimally manipulated orthobiologics, as well as
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Methoxyfuranocoumarins of Natural Origin-Updating Biological Activity Research and Searching for New Directions-A Review. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-19 Magdalena Bartnik
Plant secondary metabolites, including furanocoumarins, have attracted attention for decades as active molecules with therapeutic potential, especially those occurring in a limited number of species as evolutionarily specific and chemotaxonomically important. The most famous methoxyfuranocoumarins (MFCs), bergapten, xanthotoxin, isopimpinellin, phellopterin, byakangelicol, byakangelicin, isobergapten
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Anti-Neuroinflammatory Effects of Arecae pericarpium on LPS-Stimulated BV2 Cells. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-19 Hyeon-Gyu Cho, Dong-Uk Kim, Jin-Young Oh, Sung-Joo Park, Bitna Kweon, Gi-Sang Bae
Arecae pericarpium (AP), the fruit peel of the betel palm, is a traditional Oriental herbal medicine. AP is used to treat various diseases and conditions, such as ascites, edema, and urinary retention, in traditional Korean medicine. Recent studies have demonstrated its anti-obesity and antibacterial effects; however, its anti-neuroinflammatory effects have not yet been reported. Therefore, we investigated
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Epoxyalcohol Synthase Branch of Lipoxygenase Cascade. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-18 Yana Y Toporkova, Elena O Smirnova, Svetlana S Gorina
Oxylipins are one of the most important classes of bioregulators, biosynthesized through the oxidative metabolism of unsaturated fatty acids in various aerobic organisms. Oxylipins are bioregulators that maintain homeostasis at the cellular and organismal levels. The most important oxylipins are mammalian eicosanoids and plant octadecanoids. In plants, the main source of oxylipins is the lipoxygenase
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Growth, Survival, and Intestinal Health Alterations in Mediterranean Yellowtail (Seriola dumerili) Due to Alternatives to Fishmeal and Fish Oil. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-17 Maria Consolación Milián-Sorribes, Silvia Martínez-Llorens, David S Peñaranda, Ignacio Jauralde, Miguel Jover-Cerdá, Ana Tomás-Vidal
Fishmeal and fish oil substitution in aquafeeds might have adverse effects on fish growth and health, mainly in carnivorous species, such as Mediterranean yellowtail (Seriola dumerili). Mediterranean yellowtail shows great potential as an alternative aquaculture species due to its fast growth and high price on the market, but the need for high-quality protein and fatty acid content in its diets is
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Molecular Profiling of Circulating Tumour Cells and Circulating Tumour DNA: Complementary Insights from a Single Blood Sample Utilising the Parsortix® System. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-17 Gabrielle Wishart, Amy Templeman, Francesca Hendry, Karen Miller, Anne-Sophie Pailhes-Jimenez
The study of molecular drivers of cancer is an area of rapid growth and has led to the development of targeted treatments, significantly improving patient outcomes in many cancer types. The identification of actionable mutations informing targeted treatment strategies are now considered essential to the management of cancer. Traditionally, this information has been obtained through biomarker assessment
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Identification of the Candidate mGlu2 Allosteric Modulator THRX-195518 through In Silico Method and Evaluation of Its Neuroprotective Potential against Glutamate-Induced Neurotoxicity in SH-SY5Y Cell Line. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-17 Fadime Canbolat, Nigar Kantarci-Carsibasi, Sevim Isik, Suhair Rami Mohammed Shamshir, Münteha Girgin
Glutamate (Glu) toxicity has been an important research topic in toxicology and neuroscience studies. In vitro and in vivo studies have shown that Group II metabotropic Glu2 (mGlu2) activators have cell viability effects. This study aims to determine a candidate ligand with high mGlu2 allosteric region activity among cytotoxicity-safe molecules using the in silico positioning method and to evaluate
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The Role of Nicotinic Receptors on Ca2+ Signaling in Bovine Chromaffin Cells. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-17 Amparo Gil, Virginia González-Vélez, Luis Miguel Gutiérrez, José Villanueva
Chromaffin cells have been used as a physiological model to understand neurosecretion in mammals for many years. Nicotinic receptors located in the cells' membrane are stimulated by acetylcholine, and they participate in the exocytosis of chromaffin granules, releasing catecholamines in response to stress. In this work, we discuss how the participation of nicotinic receptors and the localization of
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The Role of the Aryl Hydrocarbon Receptor in Vascular Factors Related to Preeclampsia in a Smoking Mouse Model. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-16 Ho-Yeon Kim, Ye-Seon Seok, Hye-Yeon Moon, Geum-Joon Cho, Ki-Hoon Ahn, Soon-Cheol Hong, Min-Jeong Oh, Hai-Joong Kim
Smoking cigarettes is known to lower the risk of preeclampsia. The objective of this study is to evaluate the effect of smoking on the expression of soluble FMS-like tyrosine kinase-1 (sFlt-1), vascular endothelial growth factor (VEGF), and endoglin (sEng)-1 and the role of the aryl hydrocarbon receptor (AhR) in pregnant mice. We developed a smoking mouse model using a gas-filling system. One or two
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A Potential Role for the Receptor for Advanced Glycation End-Products (RAGE) in the Development of Secondhand Smoke-Induced Chronic Sinusitis. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-13 Hannah Robin, Courtney Trudeau, Adam Robbins, Emily Chung, Erum Rahman, Olivia Gangmark-Strickland, Frank W Licari, Duane R Winden, Dan L Orr, Juan A Arroyo, Paul R Reynolds
Chronic sinusitis (CS) is characterized by sinonasal inflammation, mucus overproduction, and edematous mucosal tissue. CS impacts one in seven adults and estimates suggest up to 15% of the general U.S. population may be affected. This research sought to assess a potential role for receptors for advanced glycation end-products (RAGE), an inflammatory receptor expressed in tissues exposed to secondhand
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Thrombomodulin Serum Levels-A Predictable Biomarker for the Acute Onset of Ischemic Stroke. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-12 Andrei-Lucian Zaharia, Dana Tutunaru, Violeta Diana Oprea, Claudiu Elisei Tănase, Ana Croitoru, Bianca Stan, Doina Carina Voinescu, Ana-Maria Ionescu, Camelia Alexandra Coadǎ, Mihaiela Lungu
The early diagnosis of acute ischemic stroke (AIS) can be challenging in cases presenting with a scarcity of clinical signs, normal cerebral imaging in early stages and a lack of specific serum markers. Thrombomodulin has been shown to be associated with cerebrovascular ischemic events and can be considered an important biomarker for the acute onset of ischemic stroke. In our study, we compared the
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Phenotyping and Exploitation of Kompetitive Allele-Specific PCR Assays for Genes Underpinning Leaf Rust Resistance in New Spring Wheat Mutant Lines. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-12 Saule Kenzhebayeva, Shynarbek Mazkirat, Sabina Shoinbekova, Saule Atabayeva, Alfia Abekova, Nargul Omirbekova, Gulina Doktyrbay, Saltant Asrandina, Dinara Zharassova, Aigul Amirova, Albrecht Serfling
Leaf rust (Puccinia triticina Eriks) is a wheat disease causing substantial yield losses in wheat production globally. The identification of genetic resources with permanently effective resistance genes and the generation of mutant lines showing increased levels of resistance allow the efficient incorporation of these target genes into germplasm pools by marker-assisted breeding. In this study, new
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The Identification of New c-FLIP Inhibitors for Restoring Apoptosis in TRAIL-Resistant Cancer Cells. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-12 Katherine Yaacoub, Rémy Pedeux, Pierre Lafite, Ulrich Jarry, Samia Aci-Sèche, Pascal Bonnet, Richard Daniellou, Thierry Guillaudeux
The catalytically inactive caspase-8-homologous protein, c-FLIP, is a potent antiapoptotic protein highly expressed in various types of cancers. c-FLIP competes with caspase-8 for binding to the adaptor protein FADD (Fas-Associated Death Domain) following death receptors' (DRs) activation via the ligands of the TNF-R family. As a consequence, the extrinsic apoptotic signaling pathway involving DRs
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Pathological and Therapeutical Implications of Pyroptosis in Psoriasis and Hidradenitis Suppurativa: A Narrative Review. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-11 Piotr K Krajewski, Maria Tsoukas, Jacek C Szepietowski
This manuscript explores the role of pyroptosis, an inflammatory programmed cell death, in the pathogenesis of two chronic dermatoses, psoriasis and hidradenitis suppurativa (HS). The diseases, though clinically diverse, share common pathogenetic pathways involving the unbalanced interaction between the adaptive and innate immune systems. This review focuses on the molecular changes in psoriatic and
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Sodium Dodecyl Sulfate Analogs as a Potential Molecular Biology Reagent. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-09 Tsutomu Arakawa, Takako Niikura, Yoshiko Kita, Teruo Akuta
In this study, we review the properties of three anionic detergents, sodium dodecyl sulfate (SDS), Sarkosyl, and sodium lauroylglutamate (SLG), as they play a critical role in molecular biology research. SDS is widely used in electrophoresis and cell lysis for proteomics. Sarkosyl and, more frequently, SDS are used for the characterization of neuropathological protein fibrils and the solubilization
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Phosphorylation Codes in IRS-1 and IRS-2 Are Associated with the Activation/Inhibition of Insulin Canonical Signaling Pathways. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-09 Anabel Martínez Báez, Guadalupe Ayala, Adolfo Pedroza-Saavedra, Hilda M González-Sánchez, Lilia Chihu Amparan
Insulin receptor substrates 1 and 2 (IRS-1 and IRS-2) are signaling adaptor proteins that participate in canonical pathways, where insulin cascade activation occurs, as well as in non-canonical pathways, in which phosphorylation of substrates is carried out by a diverse array of receptors including integrins, cytokines, steroid hormones, and others. IRS proteins are subject to a spectrum of post-translational
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Isocorydine Exerts Anticancer Activity by Disrupting the Energy Metabolism and Filamentous Actin Structures of Oral Squamous Carcinoma Cells. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-09 Qiaozhen Zhou, Qianqian Zhang, Lingzi Liao, Qian Li, Huidan Qu, Xinyu Wang, Ying Zhou, Guangzeng Zhang, Mingliang Sun, Kailiang Zhang, Baoping Zhang
Isocorydine (ICD) exhibits strong antitumor effects on numerous human cell lines. However, the anticancer activity of ICD against oral squamous cell carcinoma (OSCC) has not been reported. The anticancer activity, migration and invasion ability, and changes in the cytoskeleton morphology and mechanical properties of ICD in OSCC were determined. Changes in the contents of reactive oxygen species (ROS)
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Comparison of Retinal Metabolic Activity and Structural Development between rd10 Mice and Normal Mice Using Multiphoton Fluorescence Lifetime Imaging Microscopy. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-06 Erin Su, Niranjana Kesavamoorthy, Jason A Junge, Mengmei Zheng, Cheryl Mae Craft, Hossein Ameri
Fluorescence lifetime imaging microscopy (FLIM) is a technique that analyzes the metabolic state of tissues based on the spatial distribution of fluorescence lifetimes of certain interacting molecules. We used multiphoton FLIM to study the metabolic state of developing C57BL6/J and rd10 retinas based on the fluorescence lifetimes of free versus bound nicotinamide adenine dinucleotide and nicotinamide
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Cytotoxic Effect of Andrographis paniculata Associated with 2-Aminoethyl Dihydrogen Phosphate in Triple-Negative Breast Cells. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-05 Rosa Andrea Nogueira Laiso, Julia Carolina Ferreira, Rose Eli Grassi Rici, Laertty Garcia de Sousa Cabral, Durvanei Augusto Maria
Cancer stands out as a major global public health concern and a significant impediment to increasing life expectancy worldwide. Natural bioactives derived from plants are renowned for their efficacy in treating various types of cancer. Andrographis paniculata (Burm.f.) is a well-known plant traditionally employed in diverse medical systems across the globe. The 2-AEH2P monophosphoester, a molecule
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Production of Recombinant Single-Chain Eel Luteinizing Hormone and Follicle-Stimulating Hormone Analogs in Chinese Hamster Ovary Suspension Cell Culture. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-05 Munkhzaya Byambaragchaa, Sang-Gwon Kim, Sei Hyun Park, Min Gyu Shin, Shin-Kwon Kim, Myung-Hwa Kang, Kwan-Sik Min
We produced rec-single chain eel luteinizing (rec-eel LH) and follicle-stimulating (rec- eel FSH) hormones displaying high biological activity in Chinese hamster ovary suspension (CHO-S) cells. We constructed several mutants, in which a linker, including an O-linked glycosylated carboxyl-terminal peptide (CTP) of an equine chorionic gonadotropin (eCG) β-subunit, was attached between the β- and α-subunit
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AG1® Induces a Favorable Impact on Gut Microbial Structure and Functionality in the Simulator of Human Intestinal Microbial Ecosystem® Model. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-05 Trevor O Kirby, Philip A Sapp, Jeremy R Townsend, Marlies Govaert, Cindy Duysburgh, Massimo Marzorati, Tess M Marshall, Ralph Esposito
Modulation of the human gut microbiome has become an area of interest in the nutraceutical space. We explored the effect of the novel foundational nutrition supplement AG1® on the composition of human microbiota in an in vitro experimental design. Employing the Simulator of Human Intestinal Microbial Ecosystem (SHIME®) model, AG1® underwent digestion, absorption, and subsequent colonic microenvironment
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The Development of a CRISPR-FnCpf1 System for Large-Fragment Deletion and Multiplex Gene Editing in Acinetobacter baumannii. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-05 Shuai Wang, Yue Ding, Hua Rong, Yu Wang
Acinetobacter baumannii is a low-GC-content Gram-negative opportunistic pathogen that poses a serious global public health threat. Convenient and rapid genetic manipulation is beneficial for elucidating its pathogenic mechanisms and developing novel therapeutic methods. In this study, we report a new CRISPR-FnCpf1-based two-plasmid system for versatile and precise genome editing in A. baumannii. After
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Harnessing Natural Polymers for Nano-Scaffolds in Bone Tissue Engineering: A Comprehensive Overview of Bone Disease Treatment. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-05 Sushmita Saurav, Prashish Sharma, Anil Kumar, Zeba Tabassum, Madhuri Girdhar, Narsimha Mamidi, Anand Mohan
Numerous surgeries are carried out to replace tissues that have been harmed by an illness or an accident. Due to various surgical interventions and the requirement of bone substitutes, the emerging field of bone tissue engineering attempts to repair damaged tissues with the help of scaffolds. These scaffolds act as template for bone regeneration by controlling the development of new cells. For the
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Investigating Cox-2 and EGFR as Biomarkers in Canine Oral Squamous Cell Carcinoma: Implications for Diagnosis and Therapy. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-04 Rita Files, Catarina Santos, Felisbina L Queiroga, Filipe Silva, Leonor Delgado, Isabel Pires, Justina Prada
Oral squamous cell carcinoma (OSCC) is a common and highly aggressive dog tumor known for its local invasiveness and metastatic potential. Understanding the molecular mechanisms driving the development and progression of OSCC is crucial for improving diagnostic and therapeutic strategies. Additionally, spontaneous oral squamous cell carcinomas in dogs are an excellent model for studying human counterparts
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Rapid Visual Detection of Elite Erect Panicle Dense and Erect Panicle 1 Allele for Marker-Assisted Improvement in Rice (Oryza sativa L.) Using the Loop-Mediated Isothermal Amplification Method. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-04 Yonghang Tian, Xiyi Chen, Peizhou Xu, Yuping Wang, Xianjun Wu, Kun Wu, Xiangdong Fu, Yaoxian Chin, Yongxiang Liao
Molecular-assisted breeding is an effective way to improve targeted agronomic traits. dep1 (dense and erect panicle 1) is a pleiotropic gene that regulates yield, quality, disease resistance, and stress tolerance, traits that are of great value in rice (Oryza sativa L.) breeding. In this study, a colorimetric LAMP (loop-mediated isothermal amplification) assay was developed for the detection of the
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Suppression of UVB-Induced MMP-1 Expression in Human Skin Fibroblasts Using Lysate of Lactobacillus iners Derived from Korean Women's Skin in Their Twenties. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-04 Jin-Sung Lee, Jin-Woo Min, Seong-Bong Gye, Yong-Woo Kim, Hee-Cheol Kang, Yoon-Seo Choi, Won-Sang Seo, Bun-Yeoul Lee
The process of skin aging is intricate, involving intrinsic aging, influenced by internal factors, and extrinsic aging, mainly caused by exposure to UV radiation, resulting in photoaging. Photoaging manifests as skin issues such as wrinkles and discoloration. The skin microbiome, a diverse community of microorganisms on the skin's surface, plays a crucial role in skin protection and can be affected
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Molecular Foundations of Inflammatory Diseases: Insights into Inflammation and Inflammasomes. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-03 Mi Eun Kim, Jun Sik Lee
Inflammatory diseases are a global health problem affecting millions of people with a wide range of conditions. These diseases, including inflammatory bowel disease (IBD), rheumatoid arthritis (RA), osteoarthritis (OA), gout, and diabetes, impose a significant burden on patients and healthcare systems. A complicated interaction between genetic variables, environmental stimuli, and dysregulated immune
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The Effect of Liraglutide on Axon Regeneration and Functional Recovery after Peripheral Nerve Lesion. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-02 Mehmet Burak Yalçın, Ejder Saylav Bora, Oytun Erbaş
Peripheral nerve injuries inflict severe consequences, necessitating innovative therapeutic strategies. This study investigates the potential of liraglutide, a glucagon-like peptide-1 receptor agonist, in mitigating the consequences of peripheral nerve injury. The existing treatment methods for such injuries underscore the importance of ongoing translational research efforts. Thirty adult Wistar rats
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Construction of LncRNA-Related ceRNA Networks in Longissimus Dorsi Muscle of Jinfen White Pigs at Different Developmental Stages. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-02 Shouyuan Wang, Mingyue Shi, Yunting Zhang, Jin Niu, Wenxia Li, Jiale Yuan, Chunbo Cai, Yang Yang, Pengfei Gao, Xiaohong Guo, Bugao Li, Chang Lu, Guoqing Cao
The development of skeletal muscle in pigs might determine the quality of pork. In recent years, long non-coding RNAs (lncRNAs) have been found to play an important role in skeletal muscle growth and development. In this study, we investigated the whole transcriptome of the longissimus dorsi muscle (LDM) of Jinfen White pigs at three developmental stages (1, 90, and 180 days) and performed a comprehensive
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Maternal Influenza and Offspring Neurodevelopment. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-02 Marya Egorova, Vladimir Egorov, Yana Zabrodskaya
This review examines the complex interactions between maternal influenza infection, the immune system, and the neurodevelopment of the offspring. It highlights the importance of high-quality studies to clarify the association between maternal exposure to the virus and neuropsychiatric disorders in the offspring. Additionally, it emphasizes that the development of accurate animal models is vital for
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Physiological and Transcriptomic Analyses Reveal Commonalities and Specificities in Wheat in Response to Aluminum and Manganese. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-02 Daozhen Luo, Chunnuan Xian, Wenjie Zhang, Ying Qin, Qing Li, Muhammad Usman, Shiheng Sun, Yongxiu Xing, Dengfeng Dong
Aluminum (Al) and manganese (Mn) toxicity are the top two constraints of crop production in acid soil. Crops have evolved common and specific mechanisms to tolerate the two stresses. In the present study, the responses (toxicity and tolerance) of near-isogenic wheat lines (ET8 and ES8) and their parents (Carazinho and Egret) to Al and Mn were compared by determining the physiological parameters and
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Quantitative Analysis and Molecular Docking Simulation of Flavonols from Eruca sativa Mill. and Their Effect on Skin Barrier Function. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-02 Jihye Park, Wonchul Choi, Jayoung Kim, Hye Won Kim, Jee-Young Lee, Jongsung Lee, Bora Kim
Eruca sativa is a commonly used edible plant in Italian cuisine. E. sativa 70% ethanol extract (ES) was fractionated with five organic solvents, including n-hexane (EHex), chloroform (ECHCl3), ethyl acetate (EEA), n-butyl alcohol (EBuOH), and water (EDW). Ethyl acetate fraction (EEA) had the highest antioxidant activity, which was correlated with the total polyphenol and flavonoid content. ES and EEA
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Beeswax Alcohol Prevents Low-Density Lipoprotein Oxidation and Demonstrates Antioxidant Activities in Zebrafish Embryos and Human Subjects: A Clinical Study. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-02 Kyung-Hyun Cho, Seung-Hee Baek, Hyo-Seon Nam, Ashutosh Bahuguna, Luis Ernesto López-González, Iván Rodríguez-Cortina, José Illnait-Ferrer, Julio César Fernández-Travieso, Vivian Molina-Cuevas, Yohani Pérez-Guerra, Ambar Oyarzabal Yera, Sarahi Mendoza-Castaño
Oxidative stress is one of the primary instigators of the onset of various human ailments, including cancers, cardiovascular diseases, and dementia. Particularly, oxidative stress severely affects low-density lipid & protein (LDL) oxidation, leading to several detrimental health effects. Therefore, in this study, the effect of beeswax alcohol (BWA) was evaluated in the prevention of LDL oxidation,
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Genome-Wide Identification and Expression Analyses of the FAR1/FHY3 Gene Family Provide Insight into Inflorescence Development in Maize. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-02 Huaijun Tang, De Jing, Cheng Liu, Xiaoqing Xie, Lei Zhang, Xunji Chen, Changyu Li
As transcription factors derived from transposase, FAR-RED IMPAIRED RESPONSE1 (FAR1) and its homolog FHY3 play crucial roles in the regulation of light signaling and various stress responses by coordinating the expression of downstream target genes. Despite the extensive investigation of the FAR1/FHY3 family in Arabidopsis thaliana and other species, a comprehensive examination of these genes in maize
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Effect of Functional Inhibition of BACE1 on Sensitization to γ-Irradiation in Cancer Cells. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-02 Keitaro Nakamoto, Sota Kikuhara, Hiroaki Fujimori, Barkha Saraswat, Zhongming Gao, Ankitha Vadi Velu, Zongxiang Zhang, Ying Tong, Shoji Imamichi, Tadashige Nozaki, Yasufumi Murakami, Mitsuko Masutani
Developing strategies for the radiosensitization of cancer cells by the inhibition of genes, which harbor low toxicity to normal cells, will be useful for improving cancer radiotherapy. Here, we focused on a β-site of amyloid precursor protein (APP)-cleaving enzyme 1 (BACE1; β-secretase, memapsin-2). By functional inhibition of this peptidase by siRNA, it has also recently been shown that the DNA strand
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Single-Cell RNA Sequencing Reveals Alterations in Patient Immune Cells with Pulmonary Long COVID-19 Complications. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2024-01-02 Kristīne Vaivode, Rihards Saksis, Helēna Daiga Litvina, Helvijs Niedra, Marta Līva Spriņģe, Una Krūmiņa, Jānis Kloviņš, Vita Rovite
Since the emergence of the COVID-19 pandemic, the effects of SARS-CoV-2 have been extensively researched. While much is already known about the acute phase of the infection, increasing attention has turned to the prolonged symptoms experienced by a subset of individuals, commonly referred to as long COVID-19 patients. This study aims to delve deeper into the immune landscape of patients with prolonged
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Bitter Phytochemicals as Novel Candidates for Skin Disease Treatment. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2023-12-30 Teodora-Cristiana Grădinaru, Adelina Vlad, Marilena Gilca
Skin diseases represent a global healthcare challenge due to their rising incidence and substantial socio-economic burden. While biological, immunological, and targeted therapies have brought a revolution in improving quality of life and survival rates for certain dermatological conditions, there remains a stringent demand for new remedies. Nature has long served as an inspiration for drug development
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Characterization of the mIF4G Domains in the RNA Surveillance Protein Upf2p. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2023-12-29 Edgardo M Colón, Luis A Haddock, Clarivel Lasalde, Qishan Lin, Juan S Ramírez-Lugo, Carlos I González
Thirty percent of all mutations causing human disease generate mRNAs with premature termination codons (PTCs). Recognition and degradation of these PTC-containing mRNAs is carried out by the mechanism known as nonsense-mediated mRNA decay (NMD). Upf2 is a scaffold protein known to be a central component of the NMD surveillance pathway. It harbors three middle domains of eukaryotic initiation factor
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Recent Advances in Liver Tissue Engineering as an Alternative and Complementary Approach for Liver Transplantation. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2023-12-29 Dileep G Nair, Ralf Weiskirchen
Acute and chronic liver diseases cause significant morbidity and mortality worldwide, affecting millions of people. Liver transplantation is the primary intervention method, replacing a non-functional liver with a functional one. However, the field of liver transplantation faces challenges such as donor shortage, postoperative complications, immune rejection, and ethical problems. Consequently, there
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Integrating Metabolomics and Network Pharmacology to Decipher the Hepatoprotective Effect Mechanisms of Magnesium Isoglycyrrhizinate Injection. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2023-12-29 Yihua Zhang, Hui Li, Xueli Liu, Qiang Wang, Dong Zhao, Ming Su, Zhixin Jia, Shigang Shen
This study aimed to explore the liver protective effects of a fourth-generation glycyrrhizic acid product (magnesium isoglycyrrhizinate injection, MII) in the treatment of mice with drug-induced liver injury-specifically, to determine its effects on plasma metabolites. Moreover, the possible mechanism of its intervention in lipid metabolism and amino acid metabolism through the liver protective effect
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Anti-Staphylococcal, Anti-Candida, and Free-Radical Scavenging Potential of Soil Fungal Metabolites: A Study Supported by Phenolic Characterization and Molecular Docking Analysis. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2023-12-28 Amal A Al Mousa, Mohamed E Abouelela, Nadaa S Al Ghamidi, Youssef Abo-Dahab, Hassan Mohamed, Nageh F Abo-Dahab, Abdallah M A Hassane
Staphylococcus and Candida are recognized as causative agents in numerous diseases, and the rise of multidrug-resistant strains emphasizes the need to explore natural sources, such as fungi, for effective antimicrobial agents. This study aims to assess the in vitro anti-staphylococcal and anti-candidal potential of ethyl acetate extracts from various soil-derived fungal isolates. The investigation
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Natural History of Influenza B Virus-Current Knowledge on Treatment, Resistance and Therapeutic Options. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2023-12-26 Ghayyas Ud Din, Kinza Hasham, Muhammad Nabeel Amjad, Yihong Hu
Influenza B virus (IBV) significantly impacts the health and the economy of the global population. WHO global health estimates project 1 billion flu cases annually, with 3 to 5 million resulting in severe disease and 0.3 to 0.5 million influenza-related deaths worldwide. Influenza B virus epidemics result in significant economic losses due to healthcare expenses, reduced workforce productivity, and
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Biosynthesis of Gamma-Aminobutyric Acid (GABA) by Lactiplantibacillus plantarum in Fermented Food Production. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2023-12-26 Massimo Iorizzo, Gianluca Paventi, Catello Di Martino
In recent decades, given the important role of gamma-aminobutyric acid (GABA) in human health, scientists have paid great attention to the enrichment of this chemical compound in food using various methods, including microbial fermentation. Moreover, GABA or GABA-rich products have been successfully commercialized as food additives or functional dietary supplements. Several microorganisms can produce
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Antitumor Immunity: Role of NK Cells and Extracellular Vesicles in Cancer Immunotherapy. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2023-12-25 Angelina E Prokopeva, Charles C Emene, Marina O Gomzikova
The immune system plays a crucial role in recognizing and eliminating altered tumor cells. However, tumors develop mechanisms to evade the body's natural immune defenses. Therefore, methods for specifically recognizing/targeting tumor cells, for instance, through the activation, directed polarization, and training of immune cells, have been developed based on the body's immune cells. This strategy
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Comparative Transcriptome Analyses Provide New Insights into the Evolution of Divergent Thermal Resistance in Two Eel Gobies. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2023-12-25 Jing Liu, Tianwei Liu, Yantao Liu, Yuzhen Wang, Liqin Liu, Li Gong, Bingjian Liu, Zhenming Lü
Adaptation to thermal conditions in tidal mudflats always involves tolerating frequent fluctuations and often extreme environmental temperatures. Regulation of gene expression plays a fundamental role in the evolution of these thermal adaptations. To identify the key gene regulatory networks associated with the thermal adaptation, we investigated the capability of cold tolerance, as well as the transcriptomic
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Identification of Aly1 and Aly2 as Modulators of Cytoplasmic pH in Saccharomyces cerevisiae. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2023-12-25 Guoyong Liu, Xiuli Han, Xiang Yu, Yu Wang, Jinbiao Ma, Yongqing Yang
The regulation of intracellular pH in yeast (Saccharomyces cerevisiae) cells is critical for cell function and viability. In yeast, protons (H+) can be excreted from the cell by plasma membrane ATPase PMA1 and pumped into vacuoles by vacuolar H+-ATPase. Because PMA1 is critical to the survival of yeast cells, it is unknown whether other compensatory components are involved in pH homeostasis in the
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Thymoquinone: A Promising Therapeutic Agent for the Treatment of Colorectal Cancer. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2023-12-23 Natalia Kurowska, Marcel Madej, Barbara Strzalka-Mrozik
Colorectal cancer (CRC) is one of the most commonly diagnosed cancers and is responsible for approximately one million deaths each year. The current standard of care is surgical resection of the lesion and chemotherapy with 5-fluorouracil (5-FU). However, of concern is the increasing incidence in an increasingly younger patient population and the ability of CRC cells to develop resistance to 5-FU.
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An Unusual Presentation of Novel Missense Variant in PAX6 Gene: NM_000280.4:c.341A>G, p.(Asn114Ser). Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2023-12-22 Tatyana A Vasilyeva, Natella V Sukhanova, Olga V Khalanskaya, Andrey V Marakhonov, Nikolai S Prokhorov, Vitaly V Kadyshev, Nikolay A Skryabin, Sergey I Kutsev, Rena A Zinchenko
This study investigates a unique and complex eye phenotype characterized by minimal iris defects, foveal hypoplasia, optic nerve coloboma, and severe posterior segment damage. Through genetic analysis and bioinformatic tools, a specific nonsynonymous substitution, p.(Asn114Ser), within the PAX6 gene's paired domain is identified. Although this substitution is not in direct contact with DNA, its predicted
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Analysis of Somatic Mutations in the TCGA-LIHC Whole Exome Sequence to Identify the Neoantigen for Immunotherapy in Hepatocellular Carcinoma. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2023-12-22 Swetha Pulakuntla, Khajamohiddin Syed, Vaddi Damodara Reddy
There are numerous clinically proven methods for treating cancer worldwide. Immunotherapy has been used to treat cancer with significant success in the current studies. The purpose of this work is to identify somatically altered target gene neoantigens and investigate liver cancer-related immune cell interaction and functional changes for potential immunotherapy in future clinical trials. Clinical
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Arsenic Trioxide Decreases Lymphangiogenesis by Inducing Apoptotic Pathways and Inhibition of Important Endothelial Cell Receptors. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2023-12-21 Igor Hrgovic, Eva Zöller, Monika Doll, Tsige Hailemariam-Jahn, Thilo Jakob, Roland Kaufmann, Markus Meissner, Johannes Kleemann
Tumor-induced lymphangiogenesis is strongly associated with the formation of tumor metastasis. Therefore, the regulation of lymphangiogenesis offers a promising target in cancer therapy. Arsenic trioxide (ATO) is highly effective in the treatment of patients with acute promyelocytic leukemia (APL). As ATO mediates anti-angiogenic effects on endothelial and tumor cells, we aimed to explore the impact
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Insights into the Effects of Hydroxycinnamic Acid and Its Secondary Metabolites as Antioxidants for Oxidative Stress and Plant Growth under Environmental Stresses. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2023-12-21 Sindiswa Khawula, Arun Gokul, Lee-Ann Niekerk, Gerhard Basson, Marshall Keyster, Mihlali Badiwe, Ashwil Klein, Mbukeni Nkomo
Plant immobility renders plants constantly susceptible to various abiotic and biotic stresses. Abiotic and biotic stresses are known to produce reactive oxygen species (ROS), which cause comparable cellular secondary reactions (osmotic or oxidative stress), leading to agricultural productivity constraints worldwide. To mitigate the challenges caused by these stresses, plants have evolved a variety
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Multiplex Microarrays in 96-Well Plates Photoactivated with 4-Azidotetrafluorobenzaldehyde for the Identification and Quantification of β-Lactamase Genes and Their RNA Transcripts. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2023-12-20 Mariya M Ulyashova, Galina V Presnova, Anna A Filippova, Vitaly G Grigorenko, Alexey M Egorov, Maya Yu Rubtsova
Antibiotic-resistant bacteria represent a global issue that calls for novel approaches to diagnosis and treatment. Given the variety of genetic factors that determine resistance, multiplex methods hold promise in this area. We developed a novel method to covalently attach oligonucleotide probes to the wells of polystyrene plates using photoactivation with 4-azidotetrafluorobenzaldehyde. Then, it was
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Gallic Acid Protects from Sepsis-Induced Acute Lung Injury. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2023-12-19 Süleyman Kardaş, Osman Sezer Çınaroğlu, Ejder Saylav Bora, Oytun Erbaş
Sepsis, a leading global cause of morbidity and mortality, involves multiple organ dysfunction syndromes driven by free radical-mediated processes. Uncontrolled inflammation in early sepsis stages can lead to acute lung injury (ALI). Activated leukocytes generate reactive oxygen species, contributing to sepsis development. Gallic acid, a phenolic compound, is known for its antimicrobial properties
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Global Analysis of the WOX Transcription Factor Family in Akebia trifoliata. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2023-12-19 Shengpeng Chen, Huai Yang, Yongle Zhang, Chen Chen, Tianheng Ren, Feiquan Tan, Peigao Luo
Akebia trifoliata is an economically important, self-incompatible fruit tree in the Lardizabalaceae family. Asexual propagation is the main strategy used to maintain excellent agronomic traits. However, the generation of adventitious roots during asexual propagation is very difficult. To study the important role of the WUSCHEL-related homeobox (WOX) transcription factor in adventitious root growth
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Excess BAFF May Impact HIV-1-Specific Antibodies and May Promote Polyclonal Responses Including Those from First-Line Marginal Zone B-Cell Populations. Curr. Issues Mol. Biol. (IF 3.1) Pub Date : 2023-12-19 Kim Doyon-Laliberté, Matheus Aranguren, Josiane Chagnon-Choquet, Laurie-Anne Batraville, Olina Dagher, Jonathan Richard, Matteo Paniconi, Jean-Pierre Routy, Cécile Tremblay, Marie-Claude Quintal, Nathalie Brassard, Daniel E Kaufmann, Andrés Finzi, Johanne Poudrier, Michel Roger
We have previously shown that blood levels of B-cell Activating Factor (BAFF) rise relatively to disease progression status in the context of HIV-1 infection. Excess BAFF was concomitant with hyperglobulinemia and the deregulation of blood B-cell populations, notably with increased frequencies of a population sharing characteristics of transitional immature and marginal zone (MZ) B-cells, which we