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Down-regulation of NTSR3 inhibits cell growth and metastasis, as well as the PI3K-AKT and MAPK signaling pathways in colorectal cancer. Biochem. Cell Biol. (IF 2.46) Pub Date : 2020-03-03 Aihua Liu,Zhongfu Zuo,Linlin Liu,Lihua Liu
Colorectal cancer is a common malignancy. NTS receptor 3 (NTSR3) is known to play an important role in several cancers. This study examined the effects of NTSR3 on cell growth and metastasis in colorectal cancer. Western blot analysis, real-time PCR, immunofluorescence staining, MTT, cell cycle assay, cell apoptosis assay, Hoechst staining, caspase-3 and caspase-9 activity assays, cell adhesion assay
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Activity of fructose-1,6-bisphosphatase from Campylobacter jejuni. Biochem. Cell Biol. (IF 2.46) Pub Date : 2020-03-03 Adnan Ayna,Peter C E Moody
The glycolytic pathway of the enteric pathogen Campylobacter jejuni is incomplete; the absence of phosphofructokinase means that the suppression of futile cycling at this point in the glycolytic-gluconeogenic pathway might not be required, and therefore the mechanism for controlling pathway flux is likely to be quite different or absent. In this study, the characteristics of fructose-1,6-bisphosphatase
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LncRNA H19 promotes inflammatory response induced by cerebral ischemia-reperfusion injury through regulating the miR-138-5p-p65 axis. Biochem. Cell Biol. (IF 2.46) Pub Date : 2020-08-01 Hui Li,Chenglu Tang,Dan Wang
Recent studies have shown that long non-coding RNA(LncRNA) H19 is up-regulated in the brain of rats suffering from cerebral ischemia-reperfusion (I/R) injury, inducing severe disability and mortality. Little was known about the molecular mechanisms underlying the involvement of H19 in cerebral I/R injury. In this study, a rat model of I/R was induced by transient middle cerebral artery occlusion (tMCAO)
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LncRNA Gm4419 induces cell apoptosis in hepatic ischemia-reperfusion injury via regulating the miR-455-SOX6 axis. Biochem. Cell Biol. (IF 2.46) Pub Date : 2020-02-29 Dongjian Ying,Xinhua Zhou,Yi Ruan,Luoluo Wang,Xiang Wu
Long non-coding RNA (lncRNA) is known to be involved in a variety of diseases. However, the role of Gm4419 in hepatic ischemia-reperfusion (I/R) injury remains unknown. To study this, we first established a rat model of hepatic I/R, and a BRL-3A cell model of hypoxia-reoxygenation (H/R) for in vivo and in vitro studies. Staining with hematoxylin and eosin and hepatic injury scores were used to evaluate
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Notch1-Nrf2 signaling crosstalk provides myocardial protection by reducing ROS formation. Biochem. Cell Biol. (IF 2.46) Pub Date : 2020-02-18 Xue-Liang Zhou,Xia Wu,Rong-Rong Zhu,Hua Xu,Yun-Yun Li,Qi-Rong Xu,Sheng Liu,Song-Qing Lai,Xinping Xu,Li Wan,Qi-Cai Wu,Ji-Chun Liu
Both the Notch1 and Keap1-Nrf2 signaling pathways have cardioprotective effects, but the role of Notch1-Nrf2 crosstalk in myocardial ischemia-reperfusion injury is unclear. In this study, we established hypoxia-reoxygenation in neonate rat myocardial cells and employed γ-secretase inhibitor and curcumin to inhibit and activate the Notch1 and Keap1-Nrf2 signaling pathways, respectively. We found that
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LncRNA MALAT1 regulates diabetic cardiac fibroblasts through the Hippo-YAP signaling pathway. Biochem. Cell Biol. (IF 2.46) Pub Date : 2020-02-18 Jiangwen Liu,Liang Xu,Xiaorong Zhan
Diabetic cardiomyopathy (DCM) is a major diabetes-related microvascular disease. LncRNA MALAT1 is widely expressed in cardiomyocytes responding to hypoxia and high levels of glucose (high glucose). In this study, cardiac fibroblasts (CFs) were transfected with si-MALAT1 and exposed to high glucose. CFs in the high glucose groups were treated with 30 mmol/L glucose, and the control CFs were treated
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Investigation of the mechanism of action of a potent pateamine A analog, des-methyl, des-amino pateamine A (DMDAPatA). Biochem. Cell Biol. (IF 2.46) Pub Date : 2020-02-02 Sai Shilpa Kommaraju,Julieta Aulicino,Shruthi Gobbooru,Jing Li,Mingzhao Zhu,Daniel Romo,Woon-Kai Low
The natural product pateamineA (PatA) is a highly potent antiproliferative agent. PatA and the simplified analog desmethyl, desamino pateamineA (DMDAPatA) have exhibited cytotoxicity selective for rapidly proliferating cells, and have been shown to inhibit cap-dependent translation initiation through binding to eIF4A (eukaryotic initiation factor 4A) of the eIF4F complex. PatA and DMDAPatA are both
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Long noncoding RNA MALAT1 enhances the apoptosis of cardiomyocytes through autophagy modulation. Biochem. Cell Biol. (IF 2.46) Pub Date : 2020-01-27 Hao Hu,Jiawei Wu,Xiaofan Yu,Junling Zhou,Hua Yu,Likun Ma
Induction of autophagy promotes cardiomyocyte survival and confers a cardioprotective effect on acute myocardial infarction (AMI). Our previous study showed that knockdown of long noncoding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) attenuated myocardial apoptosis in mouse AMI. Herein, this study further investigated whether the mechanisms by which MALAT1 enhanced
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Reversal of P-glycoprotein-mediated multidrug resistance by novel curcumin analogues in paclitaxel-resistant human breast cancer cells. Biochem. Cell Biol. (IF 2.46) Pub Date : 2020-01-22 Lei Gao,Peiran Zhao,Yang Li,Dawei Yang,Ping Hu,Lianzhi Li,Yufeng Cheng,Hengchen Yao
Multidrug resistance (MDR) is a major obstacle for successful cancer chemotherapy, and the main cause of MDR has been attributed to overexpression of P-glycoprotein (P-gp). In this present study, four P-gp modulators (E,E)-4,6-bis(styryl)-2-(substituted amino)-pyrimidines were evaluated for their activity in a breast cancer cell line overexpressing P-gp (LCC6MDR). The four modulators displayed significantly
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Exogenous ubiquitin attenuates hypoxia/reoxygenation-induced cardiac myocyte apoptosis via the involvement of CXCR4 and modulation of mitochondrial homeostasis. Biochem. Cell Biol. (IF 2.46) Pub Date : 2020-01-22 Suman Dalal,Christopher R Daniels,Ying Li,Gary L Wright,Mahipal Singh,Krishna Singh
Exogenous ubiquitin (UB) plays a protective role in β-adrenergic receptor-stimulated and ischemia/reperfusion (I/R)-induced myocardial remodeling. Here, we report that UB treatment inhibits hypoxia/reoxygenation (H/R)-induced apoptosis in adult rat ventricular myocytes (ARVMs). The activation of Akt was elevated, whereas the activation of glycogen synthase kinase-3β was reduced in UB-treated cells
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Ankyrin-B p.S646F undergoes increased proteasome degradation and reduces cell viability in the H9c2 rat ventricular cardiomyoblast cell line. Biochem. Cell Biol. (IF 2.46) Pub Date : 2020-01-22 Lena Chen,Catherine S W Choi,Juan C Sanchez-Arias,Laura T Arbour,Leigh Anne Swayne
Ankyrin-B (AnkB) is scaffolding protein that anchors integral membrane proteins to the cardiomyocyte cytoskeleton. We recently identified an AnkB variant, AnkB p.S646F (ANK2 c.1937 C>T) associated with a phenotype ranging from predisposition for cardiac arrhythmia to cardiomyopathy. AnkB p.S646F exhibited reduced expression levels in the H9c2 rat ventricular-derived cardiomyoblast cell line relative
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MiR-1180 promotes cardiomyocyte cell cycle re-entry after injury through the NKIRAS2-NFκB pathway. Biochem. Cell Biol. (IF 2.46) Pub Date : 2020-01-19 Yuhui Ding,Liyuan Bi,Jun Wang
Heart failure (HF) is associated with a considerable number of symptoms and significantly impaired health for humans, including reduced quality of life and physical functioning. Previous studies have indicated that miRNAs have important roles in regulating the development of HF. MiR-1180 is involved in the proliferation, migration, invasiveness, and chemoresistance of cancer cells; however, the underlying
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Protective effect of hydralazine on a cellular model of Parkinson's disease: a possible role of hypoxia-inducible factor (HIF)-1α. Biochem. Cell Biol. (IF 2.46) Pub Date : 2020-05-14 Mehrnaz Mehrabani,Mohammad Hadi Nematollahi,Mojde Esmaeili Tarzi,Kobra Bahrampour Juybari,Moslem Abolhassani,Ali Mohammad Sharifi,Hamze Paseban,Mohsen Saravani,Solmaz Mirzamohammadi
Parkinson's disease (PD) is a neurodegenerative disease accompanied by a low expression level of cerebral hypoxia-inducible factor (HIF-1α). Hence, activating the hypoxia-signaling pathway may be a favorable therapeutic approach for curing PD. This study explored the efficacy of hydralazine, a well-known antihypertensive agent, for restoring the impaired HIF-1 signaling in PD, with the aid of 6-hydroxydopamine
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Proceedings of the 14th annual RiboWest conference: perspectives and outcome. Biochem. Cell Biol. (IF 2.46) Pub Date : 2020-01-14 Nehal Thakor,Ute Kothe,Hans-Joachim Wieden,Trushar R Patel
The RiboWest Conference brings together RNA researchers in Canada with the 2-fold goals of fostering internationally competitive RNA research and of training the next generation of scientists. The 14th Annual RiboWest conference (RiboWest 2018) was held at the University of Lethbridge (Lethbridge, Alberta) from June 10th to 13th, 2018. This meeting was focused on all major aspects of RNA research,
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Non-coding RNAs: what are we missing? Biochem. Cell Biol. (IF 2.46) Pub Date : 2020-02-01 Cristina Carvalho Barbosa,Sydnee H Calhoun,Hans-Joachim Wieden
Over the past two decades, the importance of small non-coding RNAs (sncRNAs) as regulatory molecules has become apparent in all three domains of life (archaea, bacteria, eukaryotes). In fact, sncRNAs play an important role in the control of gene expression at both the transcriptional and the post-transcriptional level, with crucial roles in fine-tuning cell responses during internal and external stress
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Omega-3 polyunsaturated fatty acids have beneficial effects on visceral fat in diet-induced obesity model. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-11-27 Aline Haas de Mello,Rosiane de Bona Schraiber,Mariana Pereira de Souza Goldim,Khiany Mathias,Carolini Mendes,Maria Eduarda Anastácio Borges Corrêa,Maria Luiza Gomes,Paulo Cesar Lock Silveira,Patrícia Fernanda Schuck,Fabricia Petronilho,Gislaine Tezza Rezin
This study evaluated the effects of omega-3 polyunsaturated fatty acids (PUFAs) on oxidative stress and energy metabolism parameters in the visceral fat of a high-fat-diet induced obesity model. Energy intake, body mass, and visceral fat mass were also evaluated. Male Swiss mice received either a control diet (control group) or a high-fat diet (obese group) for 6 weeks. After this period, the groups
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IM-12 activates the Wnt-β-catenin signaling pathway and attenuates rtPA-induced hemorrhagic transformation in rats after acute ischemic stroke. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-11-26 Ting Wang,Yu-Mei Duan,Qiao Fu,Tao Liu,Jin-Cheng Yu,Zhi-Yan Sui,Li Huang,Guo-Qiang Wen
Hemorrhagic transformation (HT) is a devastating complication for patients with acute ischemic stroke (AIS) who are treated with tissue plasminogen activator (tPA). HT is associated with high morbidity and mortality, but no effective treatments are currently available to reduce the risk of HT. Therefore, methods to prevent HT are urgently needed. In this study, we used IM-12, an inhibitor of glycogen
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Biochemistry and Cell Biology celebrates its 90th anniversary. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-11-26 James Davie,Chris Nelson
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PLOD2 promotes aerobic glycolysis and cell progression in colorectal cancer by upregulating HK2. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-11-19 Wenwu Du,Ning Liu,Yafeng Zhang,Xi Liu,Yuanhong Yang,Wei Chen,Yi He
The purpose of this study was to characterize the expression of procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 (PLOD2), a membrane-bound homodimeric enzyme that specifically hydroxylates lysine in the telopeptide of procollagens, and assess the clinical significance of PLOD2 in colorectal cancer (CRC). Our results show that PLOD2 is highly expressed in CRC tumor tissues and cell lines, both at
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MiR-539 inhibits the malignant behavior of breast cancer cells by targeting SP1. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-11-19 Fenglin Cai,Luhong Chen,Yuting Sun,Chunlan He,Deyuan Fu,Jinhai Tang
The aberrant expression of microRNAs (miRNAs) is involved in the initiation and progression of human cancers. In our study, we found that miR-539 was down-regulated in breast cancer tissues and cell lines. Decreased expression of miR-539 was significantly associated with lymph node metastasis in patients with breast cancer. Overexpression of miR-539 inhibited the proliferation and promoted apoptosis
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Protective effect of nitronyl nitroxide against hypoxia-induced damage in PC12 cells. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-11-05 Hongbo Luo,Wei Sun,Jin Shao,Huiping Ma,Zhengping Jia,Linlin Jing
Hypoxia induces cellular oxidative stress that is associated with neurodegenerative diseases. HPN (4'-hydroxyl-2-substituted phenyl nitronyl nitroxide), a stable nitronyl nitroxide, has excellent free radical scavenging properties. The purpose of this study was to investigate the protective effects of HPN on hypoxia-induced damage in PC12 cells. It was shown that HPN significantly attenuated hypoxia-induced
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Connecting proteins: shareable tools for reproducible interaction mapping. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-11-05 Anne-Claude Gingras
A summary of the Jeanne Manery Fisher Memorial Lecture presented at the CSMB meeting in Montreal, June 2019.
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Nicotine-upregulated miR-30a arrests cell cycle in G1 phase by directly targeting CCNE2 in human periodontal ligament cells. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-11-05 Lizheng Wu,Kuan Yang,Yajie Gui,Xiaojing Wang
The consumption of nicotine via smoking tobacco has been reported to stimulate the occurrence and progression of periodontitis. Many studies have demonstrated that nicotine prevents the regeneration of periodontal tissues primarily by inhibiting the proliferation of human periodontal ligament (PDL) cells. However, the mechanisms underlying this process are still unclear. Therefore, we investigated
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PML isoform expression and DNA break location relative to PML nuclear bodies impacts the efficiency of homologous recombination. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-10-31 Kathleen M Attwood,Jayme Salsman,Dudley Chung,Sabateeshan Mathavarajah,Carter Van Iderstine,Graham Dellaire
Promyelocytic leukemia nuclear bodies (PML NBs) are nuclear subdomains that respond to genotoxic stress by increasing in number via changes in chromatin structure. However, the role of the PML protein and PML NBs in specific mechanisms of DNA repair has not been fully characterized. Here, we have directly examined the role of PML in homologous recombination (HR) using I-SceI extrachromosomal and chromosome-based
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Long noncoding RNA HIF1A-AS2 facilitates cell survival and migration by sponging miR-33b-5p to modulate SIRT6 expression in osteosarcoma. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-10-18 Hang Lin,Zhenxu Zhao,Yi Hao,Jun He,Jian He
Long noncoding RNAs (lncRNAs) are emerging as vital regulators in various physiological and pathological processes. It was recently found that lncRNA HIF1A-AS2 could play oncogenic roles in several cancers. However, the function and regulatory mechanism of lncRNA HIF1A-AS2 in osteosarcoma (OS) remain largely unclear. In this study, we demonstrated that HIF1A-AS2 was overexpressed in OS tissues and
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MiR-126 targets IL-17A to enhance proliferation and inhibit apoptosis in high-glucose-induced human retinal endothelial cells. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-10-13 Xiujuan Chen,Xuequn Yu,Xinxiang Li,Li Li,Fang Li,Ting Guo,Cuihong Guan,Liping Miao,Guoping Cao
Diabetic retinopathy (DR) is a common complication of diabetes mellitus (DM), which results in vision loss. This study explored the role of miR-126 in high-glucose-induced human retinal endothelial cells (HRECs) and its underlying molecular mechanisms. The results showed that the expression levels of miR-126 and interleukin-17A (IL-17A) in high-glucose-induced HRECs were downregulated and upregulated
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Subcellular fractionation of frozen skeletal muscle samples. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-10-12 Pedro Rafael Firmino Dias,Paulo Guimarães Gandra,René Brenzikofer,Denise Vaz Macedo
Cell fractionation can be used to determine the localization and trafficking of proteins between cellular compartments such as the cytosol, mitochondria, and nuclei. Subcellular fractionation is usually performed immediately after tissue dissection because freezing may fragment cell membranes and induce organellar cross-contamination. Mitochondria are especially sensitive to freezing/thawing and mechanical
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MicroRNA-1298-5p inhibits cell proliferation and the invasiveness of bladder cancer cells via down-regulation of connexin 43. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-10-10 Gang Li,Longfeng Sun,Zhongyi Mu,Shibo Liu,Hongchen Qu,Qingpeng Xie,Bin Hu
MicroRNA (miR)-1298 is widely down-regulated in a variety of malignant tumors, which facilitates cell proliferation, invasiveness, and migration. However, the specific biological function of miR-1298 in bladder cancer (BC) is still unknown. Connexin 43 (Cx43) is often up-regulated in tumors. Identifying miRNAs that target Cx43 in the setting of BC will help to develop Cx43-based therapies for BC. In
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Low n-6/n-3 PUFA ratio improves inflammation and myocardial ischemic reperfusion injury. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-10-04 Caiyan Ma,Zehang Xu,Heng Lv
This study investigated the potential effect of n-6/n-3 polyunsaturated fatty acids (PUFA) on inflammation and myocardial ischemic reperfusion injury (MIRI) in rats, together with the underlying protective mechanisms, and screen out most effective ratio of n-6/n-3 within limits. The rats with pre-infarct treatment were distributed among 5 groups according to the n-6/n-3 ratio (36:1; 1:1, 5:1, 10:1
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Extracellular pyruvate kinase M2 facilitates cell migration by upregulating claudin-1 expression in colon cancer cells. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-09-23 Hyunju Kim,Seong Ho Kim,Dohyeon Hwang,Jinsu An,Hak Suk Chung,Eun Gyeong Yang,So Yeon Kim
Extensive studies have been reported the non-canonical functions of pyruvate kinase M2 (PKM2) as a kinase, transcriptional regulator, and even cell-to-cell communicator, emphasizing its importance in various signaling pathways. However, the role of secreted PKM2 in cancer progression and its signaling pathway is yet to be elucidated. In this study, we found that extracellular PKM2 enhanced the migration
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MicroRNA-24 alleviates isoflurane-induced neurotoxicity in rat hippocampus via attenuation of oxidative stress. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-09-18 Na Li,Linli Yue,Jun Wang,Zhenzhen Wan,Wenhao Bu
Several miRNAs have been recently suggested as potential therapeutic targets for anesthesia-related diseases. This study was carried out to explore the biological roles of miR-24 in isoflurane-treated rat hippocampal neurons. Isoflurane was used to induce neurotoxicity in a rat model. Gain- and loss-of-function of miR-24 was performed, and the size and Ca2+ permeability of mitochondria, as well as
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MicroRNA profiling of human myeloid angiogenic cells derived from peripheral blood mononuclear cells. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-09-04 Qiuwang Zhang,Anthony Cannavicci,Si-Cheng Dai,Chenxi Wang,Michael J B Kutryk
Human myeloid angiogenic cells (MACs), also termed early endothelial progenitor cells, play an important role in neovascularization and vascular repair. MicroRNAs (miRNAs) are a class of naturally occurring, noncoding, short (∼22 nucleotides), single-stranded RNAs that regulate gene expression post-transcriptionally. MiRNAs have been shown to regulate MAC function. A miRNA signature of MACs was described
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Genes responsive to rapamycin and serum deprivation are clustered on chromosomes and undergo reorganization within local chromatin environments. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-09-03 Zachery R Belak,Joshua A Pickering,Zoe E Gillespie,Gerald Audette,Mark Eramian,Jennifer A Mitchell,Joanna M Bridger,Anthony Kusalik,Christopher H Eskiw
We previously demonstrated that genome reorganization, through chromosome territory repositioning, occurs concurrently with significant changes in gene expression in normal primary human fibroblasts treated with the drug rapamycin, or stimulated into quiescence. Although these events occurred concomitantly, it is unclear how specific changes in gene expression relate to reorganization of the genome
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MiR-204 suppresses cell proliferation and promotes apoptosis in ovarian granulosa cells via targeting TPT1 in polycystic ovary syndrome. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-08-29 Xueqin Sun,Shan Su,Guoxiang Zhang,Hong Zhang,Xiaohui Yu
MicroRNA (miR)-204 is known to be associated with several different diseases. Polycystic ovary syndrome (PCOS) has the highest incidence rate among the endocrine disorders in females between the ages of 18 and 44. We aimed to illustrate the miR-204 function in PCOS. MiR-204 expression levels in tissue and cell were examined through RT-qPCR. Colony formation assay and MTT assay were applied to detect
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Silencing LINC00511 inhibits cell proliferation, migration, and epithelial-mesenchymal transition via the PTEN-AKT-FOXO1 signaling pathway in lung cancer. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-08-15 Lianyong Jiang,Xiao Xie,Fangbao Ding,Ju Mei,Rui Bi
Lung cancer is the most common cause of cancer-related death worldwide. Long noncoding RNAs (lncRNAs) are longer than 200 nt transcripts and are not translated into proteins. Increasing evidence has shown that lncRNAs are associated with several biological processes in cancer. However, the roles of LINC00511 in lung cancer progression remain unknown. In the present study, we confirmed that LINC00511
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RP5-1120P11.3 promotes hepatocellular carcinoma development via the miR-196b-5p-WIPF2 axis. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-07-12 Hongjun Zhai,Xinwu Zhang,Shuo Chen,Meng Fan,Shuangyu Ma,Xiaoli Sun
Hepatocellular carcinoma (HCC) remains a huge threat to human health even though the diagnosis and treatment strategies have improved rapidly in the past few decades. Increasing evidence has illustrated the critical role noncoding RNA and their regulatory network play in the pathology of HCC. Here, we identified a novel long noncoding RNA, RP5-1120P11.3, that is ectopically expressed in HCC. Further
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circ-NOTCH1 acts as a sponge of miR-637 and affects the expression of its target gene Apelin to regulate gastric cancer cell growth. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-07-05 Encui Guan,Xiaoguang Xu,Fangxi Xue
Gastric cancer (GC) is a major cause of cancer-related deaths worldwide, and has a low survival rate, low cure rate, high recurrence rate, and poor prognosis. Recent studies have indicated that circular RNAs (circRNAs) have important functions in the occurrence and progression of GC. Studies on circ-NOTCH1, which was shown to be highly expressed in GC, have indicated that miR-637 binds to circ-NOTCH1
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Atypical chromatin structure of immune-related genes expressed in chicken erythrocytes. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-07-05 Sanzida Jahan,Tasnim H Beacon,Wayne Xu,James R Davie
The major biological role of red blood cells is to carry oxygen to the tissues in the body. However, another role of the erythroid cell is to participate in the immune response. Mature erythrocytes from chickens express Toll-like receptors and several cytokines in response to stimulation of the immune system. We previously reported the application of a biochemical fractionation protocol to isolate
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MicroRNA-378-3p/5p suppresses the migration and invasiveness of oral squamous carcinoma cells by inhibiting KLK4 expression. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-07-02 Zhi Cui,Shiqun Sun,Qilin Liu,Xuechun Zhou,Siyu Gao,Peixuan Peng,Qianpeng Li
Distant metastasis frequently occurs in oral squamous cell carcinoma (OSCC) and contributes to the adverse prognosis for patients with OSCC. However, the potential mechanisms behind the metastasis have not yet been clarified. This study investigated the role of miR-378 in the migration and invasiveness of OSCC in vitro and in vivo. According to our results, the migration and invasiveness of OSCC cells
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LncRNA SNHG4 promotes the proliferation, migration, invasiveness, and epithelial-mesenchymal transition of lung cancer cells by regulating miR-98-5p. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-06-20 Yufu Tang,Lijian Wu,Mingjing Zhao,Guangdan Zhao,Shitao Mao,Lingling Wang,Shuo Liu,Xiaoge Wang
Long noncoding RNA small nucleolar RNA host gene 4 (SNHG4) is usually up-regulated in cancer and regulates the malignant behavior of cancer cells. However, its role in lung cancer remains elusive. In this study, we silenced the expression of SNHG4 in NCI-H1437 and SK-MES-1, two representative non-small-cell lung cancer cell lines, by transfecting them with siRNA (small interfering RNA) that specifically
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MiR-19a enhances cell proliferation, migration, and invasiveness through enhancing lymphangiogenesis by targeting thrombospondin-1 in colorectal cancer. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-06-14 Qian Yin,Pei-Pei Wang,Rui Peng,Hang Zhou
Colorectal cancer (CRC) is a devastating disease with high mortality and morbidity, and the underlying mechanisms of miR-19a in CRC are poorly understood. In our study, dual-luciferase reporter assays were used to evaluate the binding of miR-19a with thrombospondin-1 (THBS1). Cell viability, migration, and invasiveness were assessed using MTT, wound healing, and Transwell assays, respectively. Tube-formation
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Circ_RUSC2 upregulates the expression of miR-661 target gene SYK and regulates the function of vascular smooth muscle cells. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-06-14 Jingang Sun,Zhigang Zhang,Shuguo Yang
Many studies have identified circRNA as a prospective direction in the field of cardiovascular research. Detection of circRNA expression in different vascular smooth muscle cell (VSMC) phenotypes revealed that circ_RUSC2 is upregulated in proliferative VSMCs. Sequence analysis of circ_RUSC2 showed that there are multiple binding sites of miR-661 on circ_RUSC2, and that SYK is an important target gene
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DNA methylation and regulation of DNA methyltransferases in a freeze-tolerant vertebrate. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-05-22 Jing Zhang,Liam J Hawkins,Kenneth B Storey
The wood frog is one of the few freeze-tolerance vertebrates. This is accomplished in part by the accumulation of cryoprotectant glucose, metabolic rate depression, and stress response activation. These may be achieved by mechanisms such as DNA methylation, which is typically associated with transcriptional repression. Hyperglycemia is also associated with modifications to epigenetic profiles, indicating
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How to continually make the case for fundamental science: from the perspective of a protein kinase. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-05-21 James Robert Woodgett
The strength of the scientific process is its immunity from human frailties. The built-in error correction and robustness of principles protect and nurture truth, despite both intended and unintended errors and naivety. What it doesn't secure is understanding of how the scientific sausage is made. Here, a scientific journey revolving around a single protein that spans nearly 35 years is used to illustrate
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The impact of epigenetics on cardiovascular disease. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-05-21 Dimple Prasher,Steven C Greenway,Raja B Singh
Mortality and morbidity from cardiovascular diseases (CVDs) represents a huge burden to society. It is recognized that environmental factors and individual lifestyles play important roles in disease susceptibility, but the link between these external risk factors and our genetics has been unclear. However, the discovery of sequence-independent heritable DNA changes (epigenetics) have helped us to explain
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Label-free proteomic analysis reveals large dynamic changes to the cellular proteome upon expression of the miRNA-23a-27a-24-2 microRNA cluster. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-05-16 Ramanaguru S Piragasam,S Faraz Hussain,Steven G Chaulk,Zaeem A Siddiqi,Richard P Fahlman
In deciphering the regulatory networks of gene expression controlled by the small non-coding RNAs known as microRNAs (miRNAs), a major challenge has been with the identification of the true mRNA targets by these RNAs within the context of the enormous numbers of predicted targets for each of these small RNAs. To facilitate the system-wide identification of miRNA targets, a variety of system wide methods
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MicroRNA-211-5p promotes apoptosis and inhibits the migration of osteosarcoma cells by targeting proline-rich protein PRR11. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-05-10 Dandan Song,Kun Yang,Wei Wang,Run Tian,Haoyu Wang,Kunzheng Wang
Osteosarcoma remains fatal in adolescents and young adults, with a 5-year survival rate of less than 20%. However, the details for mechanisms that regulate osteosarcoma metastasis are poorly understood. We analyzed the expression levels of miR-211-5p in clinical samples of osteosarcoma as well as cell lines, and found that the expression of miR-211-5p was reduced in osteosarcoma. Moreover, induction
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Mitochondrial dysfunction regulates the JAK-STAT pathway via LKB1-mediated AMPK activation ER-stress-independent manner. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-05-09 Dong-Yeon Kim,Su-Geun Lim,Kyoungho Suk,Won-Ha Lee
Mitochondria affect cellular functions alone or in cooperation with other cellular organelles. Recent research has demonstrated the close relationship of mitochondria with the endoplasmic reticulum (ER), both at the physical and the functional level. In an effort to define the combined effect of mitochondrial dysfunction (MD) and ER stress in the proinflammatory activities of macrophages, the human
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Particulate matter with a diameter of ≤2.5 μm induces and enhances bleomycin-induced pulmonary fibrosis by stimulating endoplasmic reticulum stress in rat. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-05-07 Panfeng Xu,Yake Yao,Jianying Zhou
This study was designed to investigate the effect of particulate matter with a diameter of ≤2.5 μm (PM2.5) on bleomycin (BLM) induced pulmonary fibrosis. Thirty-two Sprague Dawley rats were assigned into four groups (intratracheal instillation of 500 μL of PBS (control), 2 mg/kg PM2.5, 3.5 mg/kg BLM A5, and BLM plus 2.0 mg/kg PM2.5) and were fed for 14 days. All rats were sacrificed after the study
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Sirt6 stabilizes atherosclerosis plaques by promoting macrophage autophagy and reducing contact with endothelial cells. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-05-07 Tingting Wang,Chuang Sun,Lang Hu,Erhe Gao,Congye Li,Haichang Wang,Dongdong Sun
Sirt6 has been reported to play a protective role in macrophage foam cell formation, but whether Sirt6 controls atherosclerosis plaque stability and whether it can reduce the interaction between endothelial cells and macrophages remains unclear. The aim of this study was to investigate the effect of Sirt6 on atherosclerosis plaque stability and the underlying mechanisms. We used Tie2-Cre transgenic
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Role of syndecan-1 and exogenous heparin in hepatoma sphere formation. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-05-01 Shih-Chiang Lin,Ching-Po Wu,TingTing Tseng,Yaoyun Jhang,Shao-Chen Lee
Glycosaminoglycan-modified proteoglycans play important roles in many cell activities, including cell differentiation and stem cell development. Tumor sphere formation ability is one of properties in cancer stem cells (CSCs). The correlation between CSC markers and proteoglycan remains to be clarified. Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the
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miR-628-5p promotes growth and migration of osteosarcoma by targeting IFI44L. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-04-24 Ju-Yong Wang,Ju-Qiang Wang,Shi-Bao Lu
This study investigated the role of miR-628-5p and interferon-induced protein 44-like (IFI44L) in osteosarcoma (OS) and determined whether miR-628-5p modulated OS growth by regulating IFI44L. Based on the data downloaded from Gene Expression Omnibus (GEO) database, we revealed that the expression of IFI44L was downregulated in OS and low expression of IFI44L was correlated with better prognosis of
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Long noncoding RNA XIST regulates the EGF receptor to promote TGF-β1-induced epithelial-mesenchymal transition in pancreatic cancer. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-04-23 Lei Zou,Feng-Rong Chen,Ren-Pin Xia,Hua-Wei Wang,Zhen-Rong Xie,Yu Xu,Jue-Hua Yu,Kun-Hua Wang
BACKGROUND This study focuses on the lncRNA XIST (X inactive-specific transcript), an lncRNA involved in multiple human cancers, and investigates the functional significance of XIST and the molecular mechanisms underlying the epithelial-mesenchymal transition (EMT) in pancreatic cancer (PC). METHODS Clinical specimens from 25 patients as well as 5 human PC cell lines were analyzed for XIST, YAP, and
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Propofol promotes apoptosis of colorectal cancer cells via alleviating the suppression of lncRNA HOXA11-AS on miRNA let-7i. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-04-23 Yan-Ling Ren,Wei Zhang
To date, surgical resection is the mainstay for the treatment of colorectal cancer (CRC). Propofol (2,6-diisopropylphenol), one of the most commonly used intravenous anaesthetic agents, has been reported to be involved in modulating the malignancy of a variety of human cancers. However, the underlying mechanisms remain poorly understood. In this study, using a cell counting kit (CCK-8), flow cytometry
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Effect of caveolin-1 on Stat3-ptyr705 levels in breast and lung carcinoma cells. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-04-16 Mulu Geletu,Zaid Taha,Rozanne Arulanandam,Reva Mohan,Hikmat H Assi,Maria G Castro,Ivan Robert Nabi,Patrick T Gunning,Leda Raptis
We recently demonstrated that Cav1 (caveolin-1) is a negative regulator of Stat3 (signal transducer and activator of transcription-3) activity in mouse fibroblasts and human lung carcinoma SHP77 cells. We now examined whether the cellular context may affect their levels as well as the relationship between them, by assessing Cav1 and Stat3-ptyr705 amounts in different cell lines. In MDA-MB-231, A549
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40th International Asilomar Chromatin, Chromosomes, and Epigenetics Conference. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-04-11 Zoe E Gillespie,Tanner Barkhshi,Maria Laura Sosa Ponce,Philippe T Georgel,Juan Ausió
The 40th International Asilomar Chromatin, Chromosomes, and Epigenetics Conference was held in the Asilomar Conference Grounds, Pacific Grove, California, USA, on 6-9 December 2018. The organizing committee consisted of established scientists in the fields of chromatin and epigenetics: Sally Pasion and Michael Goldman from the Biology Department, San Francisco State University, California, USA; Philippe
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MiR-4458 inhibits breast cancer cell growth, migration, and invasiveness by targeting CPSF4. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-04-10 Jianrong Wu,Juan Miao,Ye Ding,Yayun Zhang,Xiaohao Huang,Xue Zhou,Ranran Tang
Numerous studies have reported that CPSF4 is over-expressed in a large percentage of human lung cancers, and CPSF4 has been identified as a potential oncogene of human lung tumor. Downregulation of CPSF4 inhibits the proliferation and promotes the apoptosis of lung adenocarcinoma cells. A previous study by our group also found overexpression of CPSF4 in breast cancer (BC), and was closely associated
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Impact of double-stranded RNA characteristics on the activation of human 2'-5'-oligoadenylate synthetase 2 (OAS2). Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-04-09 Amit Koul,Soumya Deo,Evan P Booy,George L Orriss,Matthew Genung,Sean A McKenna
Human 2'-5' oligoadenylate synthetases (OAS) are a family of interferon-inducible proteins that, upon activation by double-stranded RNA, polymerize ATP into 2'-5' linked oligoadenylates. In this study, we probed the RNA cofactor specificity of the two smallest isozymes, OAS1 and OAS2. First, we developed a strategy for the expression and purification of recombinant human OAS2 from eukaryotic cells
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SARS coronavirus protein nsp1 disrupts localization of Nup93 from the nuclear pore complex. Biochem. Cell Biol. (IF 2.46) Pub Date : 2019-04-03 Garret N Gomez,Fareeha Abrar,Maya P Dodhia,Fabiola G Gonzalez,Anita Nag
Severe acute respiratory syndrome coronavirus nonstructural protein 1 (nsp1) is a key factor in virus-induced down-regulation of host gene expression. In infected cells, nsp1 engages in a multipronged mechanism to inhibit host gene expression by binding to the 40S ribosome to block the assembly of translationally competent ribosome, and then inducing endonucleolytic cleavage and the degradation of
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