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Study on the effect of type III recombinant humanized collagen on human vascular endothelial cells. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-11-29 Han Wang,Yonghao Xiao,Yuanguo Zhang,Zhu Meng,Chenyu Zhao,Fanshan Qiu,Chongchong Li,Zengguo Feng
METHODS The effect of hCOLII on proliferation of EA.hy926 cells was detected by MTT assay, the effect of hCOLII on cell migration was investigated by scratch assay, the effect of hCOLIII on cell cycle and apoptosis was detected by flow cytometry, the ability of hCOLII to induce angiogenesis of EA.hy926 cells was evaluated by angiogenesis assay, and the effect of hCOLII on VEGF expression was detected
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Novel chip for applying mechanical forces on human skin models under dynamic culture conditions. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-11-11 Katharina Kaiser,Jens Ahm Sørensen,Jonathan Brewer
In recent years the need for in vitro skin models as a replacement for animal studies has resulted in significant progress in the development of skin-on-a-chip models. These devices allow the fine control of the microenvironment of the model and the incorporation of chemical and physical stimuli. Here we describe the development of an easy and low-budget open-top dynamic microfluidic device for skin-on-a-chip
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Ozone improved bone dynamic of female rats using zoledronate. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-11-07 Tiburtino José de Lima Neto,Leonardo Alan Delanora,Maria Eloise de Sá Simon,Kim Henderson Carmo Ribeiro,Mariza Akemi Matsumoto,Mario Jefferson Quirino Louzada,Jamil Awad Shibli,Edilson Ervolino,Leonardo Perez Faverani
The aim of this study was to analyze the effect of ozone (OZN) therapy on the dynamics of bone tissue in ovariectomized rats treated with zoledronic acid (ZOL). Female Wistar rats aged 6 months (n = 110) were subjected to bilateral ovariectomy (OVX). At month 3 post-OVX, 10 animals were euthanized to characterize the bone tissue architecture using microtomography (micro-CT). The remaining animals were
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Potential use of extracellular vesicles in the treatment of intervertebral disc degeneration. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-11-06 Qiu-Wei Li,Ruo-Cheng Guo,Zuo-Meng Wu,Cai-Liang Shen
BACKGROUND Intervertebral disc degeneration is a major cause of low back pain, and several studies have evaluated the efficacy of extracellular vesicles in the treatment of intervertebral disc degeneration. METHODS The databases Pubmed, Embase, and Cochrane Library were systematically searched from inception to the end of 2022 to identify studies investigating the therapeutic potential of cell-derived
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Biomimetic Cardiac Fibrotic Model for Antifibrotic Drug Screening. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-11-06 Haiyan Li,Yifan Zhu,Zhe Chen,Qiaolin Ma,Ahmed I Abd-Elhamid,Bei Feng,Binbin Sun,Jinglei Wu
Cardiac fibrosis is characterized by pathological proliferation and activation of cardiac fibroblasts to myofibroblasts. Inhibition and reverse of transdifferentiation of cardiac fibroblasts to myofibroblasts is a potential strategy for cardiac fibrosis. Despite substantial progress, more effort is needed to discover effective drugs to improve and reverse cardiac fibrosis. The main reason for the slow
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Autologous Endothelial Progenitor Cells and Bioactive Factors Improve Bladder Regeneration. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-10-23 Bin Yang,Guanjie Yang,Feng Zhao,Xudong Yao,Luwei Xu,Liuhua Zhou
Insufficient vascularization is still a challenge that impedes bladder tissue engineering and results in unsatisfied smooth muscle regeneration. Since bladder regeneration is a complex articulated process, the aim of this study is to investigate whether combining multiple pathways by exploiting a combination of biomaterials, cells, and bioactive factors, contributes to the improvements of smooth muscle
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Transplantation of Induced Pluripotent Stem Cell-Derived Airway Epithelia with a Collagen Scaffold into the Nasal Cavity. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-10-18 Yuji Kitada,Hiroe Ohnishi,Norio Yamamoto,Fumihiko Kuwata,Masayuki Kitano,Keisuke Mizuno,Koichi Omori
The nasal cavity is covered with respiratory epithelia, including ciliated cells that eliminate foreign substances trapped in the mucus. In hereditary diseases such as primary ciliary dyskinesia and cystic fibrosis, respiratory epithelial functions are irreversibly impaired; however, no radical treatment has been established yet. Thus, we considered that the transplantation of normal airway epithelia
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Developing a Cell-Microcarrier Tissue-Engineered Product for Muscle Repair Using a Bioreactor System. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-11-21 Ana Luísa Cartaxo,Ana Fernandes-Platzgummer,Carlos A V Rodrigues,Ana M Melo,Katja Tecklenburg,Eva Margreiter,Richard M Day,Cláudia L da Silva,Joaquim M S Cabral
Fecal incontinence, although not life-threatening, has a high impact on the economy and patient quality of life. So far, available treatments are based on both surgical and nonsurgical approaches. These can range from changes in diet, to bowel training, or sacral nerve stimulation, but none of which provides a long-term solution. New regenerative medicine-based therapies are emerging, which aim at
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Global Research Trends in Adipose Stem Cell Tissue Engineering: A Scientometric Research. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-10-05 Yang Liu,Singjie Wong,Yunxi Han,Xuefei Li,Luying Yuan,Mengting Xiong,Li Zhao,Hao Xu,Tengteng Wang,Qianqian Liang
Adipose Stem Cell Tissue Engineering (ASCTE) has emerged as a promising field of research in recent years. To gain comprehensive insights into this field, we conducted a comprehensive bibliometric analysis using Web of Science Core Collection and various bibliometric tools, including CiteSpace, VOS viewer, and R-Bibliometrix. Our analysis focuses on the historical development and evolution of active
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Organalysis: Multifunctional Image Preprocessing and Analysis Software for Cardiac Organoid Studies. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-10-04 Jathin Pranav Singaraju,Adheesh Kadiresan,Rahul Kumar Bhoi,Angello Huerta Gomez,Zhen Ma,Huaxiao Yang
Due to a growing need in visualizing human pluripotent stem cell-derived organoids from recent advancements in the field, an efficient bulk-processing application is necessary to provide preprocessing and image analysis services. In this study, we developed Organalysis, a high-accuracy, multifunctional, and accessible application that meets these needs by providing the functionality of image manipulation
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Toward a 3D Printed Perfusable Islet Embedding Structure: Technical Notes and Preliminary Results. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-10-01 Eriselda Keshi,Peter Tang,Tobias Lam,Simon Moosburner,Luna Haderer,Anja Reutzel-Selke,Lutz Kloke,Johann Pratschke,Igor Maximilian Sauer,Karl Herbert Hillebrandt
To date, islet transplantation to treat type 1 diabetes mellitus remains unsuccessful in long-term follow-up, mainly due to failed engraftment and reconstruction of the islet niche. Alternative approaches, such as islet embedding structures (IESs) based on 3D printing have been developed. However, most of them have been implanted subcutaneously and only a few are intended for direct integration into
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Hydrothermal Extraction and Characterization of Natural Hydroxyapatite from Waste Bovine Femur Bone. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-09-29 Yi Zhang,Fei Kong,Dongze Wu,Jinjin Zhu,Shuhui Yang,Xiangdong Kong
The management of large amounts of bio-wastes, such as bovine femurs from kitchens and slaughterhouses, has long been a challenging issue. However, through the utilization of a hydrothermal process, it is possible to transform these bio-wastes into valuable products. In this study, we focused on extracting hydroxyapatite (HAp), the primary inorganic component of bovine femurs, for potential use in
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Characterization of Nerve Damage after an Injury to the Adjacent Soft Tissue: a pilot animal study. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-09-24 Nesreen Zoghoul Alsmadi,Curt Deister,Nik Agrawal,Lan Tran,Rasa Zhukauskas,Debbie Neubauer Fischer,Deana Mercer
Traumatic injuries may result in the formation of soft tissue adhesions between peripheral nerves and surrounding soft tissue. These soft tissue adhesions lead to compression and ischemic stress within fascicles due to non-pliability of adhered scar tissue, and nerve tension due to loss of nerve gliding from scar tethering. These changes in the soft tissue bed surrounding the nerve may result in axon
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A Combined Western and Bead-Based Multiplex Platform to Characterize Extracellular Vesicles. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-08-22 Josette C van Maanen,Frances C Bach,Theresa S Braun,Alberta Giovanazzi,Bas W M van Balkom,Markus Templin,Marca H M Wauben,Marianna A Tryfonidou
In regenerative medicine, extracellular vesicles (EVs) are considered as a promising cell-free approach. EVs are lipid bilayer-enclosed vesicles secreted by cells and are key players in intercellular communication. EV-based therapeutic approaches have unique advantages over the use of cell-based therapies, such as a high biological, but low immunogenic and tumorigenic potential. To analyze the purity
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Call for Special Issue Papers: Artificial Intelligence in Tissue Engineering and Biology. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-07-19 Jason L Guo,Michael Januszyk,Michael T Longaker
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Evaluation of Porcine Psoas Major as a Scaffold Material for Engineered Heart Tissues. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-08-10 Shi Shen,Stephanie Shao,Maria Papadaki,Jonathan A Kirk,Stuart G Campbell
Decellularized porcine myocardium is commonly used as scaffolding for engineered heart tissues (EHTs). However, structural and mechanical heterogeneity in the myocardium complicate production of mechanically consistent tissues. In this study, we evaluate the porcine psoas major muscle (tenderloin) as an alternative scaffold material. Head-to-head comparison of decellularized tenderloin and ventricular
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Reproducible Preparation of Primary Rat Hepatocyte Sheets Using a Thermoresponsive Culture Dish. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-08-10 Jun Kobayashi,Teruo Okano
Hepatocyte transplantation has been utilized as a therapy for congenital metabolic liver diseases such as hemophilia and for liver function support in acute liver failure. Hepatocyte sheet technology using a thermoresponsive poly(N-isopropylacrylamide) (PIPAAm)-grafted dish is expected to provide an efficient cell transplantation method because the resulting hepatocyte sheet possesses extracellular
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Harvest of Cell-Only Muscle Fibers Using Thermally Expandable Hydrogels with Adhesive Patterns. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-08-03 Yu Bin Lee,Se-Jeong Kim,Eun Mi Kim,Hayeon Byun,Heungsoo Shin
Muscle tissue engineering has been the focus of extensive research due to its potential for numerous medical applications, including ex vivo actuator development and clinical treatments. In this study, we developed a method for harvesting muscle fiber in a floatable and translocatable manner utilizing thermally expandable hydrogels with a chemically patterned polydopamine (PD) layer generated by microcontact
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Improved Production of Induced Pluripotent Stem Cells Using Dot Pattern Culture Plates. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-08-16 Yoshiki Nakashima,Hiroki Iguchi,Eiko Shimizu,Minh N T Le,Kenta Takakura,Yuta Nakamura,Teruhiko Yanagisawa,Rutvi Sanghavi,Satoshi Haneda,Masayoshi Tsukahara
The rate of cell proliferation is a crucial factor in cell production under good manufacturing practice (GMP) control. In this study, we identified a culture system for induced pluripotent cells (iPSCs) that supports cell proliferation and viability and maintains the cells in an undifferentiated state even at 8 days after seeding. This system involves the use of dot pattern culture plates that have
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Call for Special Issue Papers: Bioprinting. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-07-10 John P Fisher,Lijie Grace Zhang
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Coating 3D-Printed Bioceramics with Histatin Promotes Adhesion and Osteogenesis of Stem Cells. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-07-01 Dongyun Wang,Haiyan Wang,Yongyong Yan,Nan Wei,Richard T Jaspers,Wei Cao,Xiaoxuan Lei,Shuyi Li,Yajie Qi,Fengjun Hu,Haifeng Lan,Gang Wu
Mesenchymal stem cell and 3D printing-based bone tissue engineering present a promising technique to repair large-volume bone defects. Its success is highly dependent on cell attachment, spreading, osteogenic differentiation, and in vivo survival of stem cells on 3D-printed scaffolds. In this study, we applied human salivary histatin-1 (Hst1) to enhance the interactions of human adipose-derived stem
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Microfluidic Platform for Microparticle Fabrication and Release of a Cathepsin Inhibitor. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-08-01 Elda A Treviño,Jimmy Shah,Joseph J Pearson,Manu O Platt,Younan Xia,Johnna S Temenoff
Cathepsins are a family of cysteine proteases responsible for a variety of homeostatic functions throughout the body, including extracellular matrix remodeling, and have been implicated in a variety of degenerative diseases. However, clinical trials using systemic administration of cathepsin inhibitors have been abandoned due to side effects, so local delivery of cathepsin inhibitors may be advantageous
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The Upscale Manufacture of Chondrocytes for Allogeneic Cartilage Therapies. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-09-01 Charlotte H Hulme,John K Garcia,Claire Mennan,Jade Perry,Sally Roberts,Kevin Norris,Duncan Baird,Larissa Rix,Robin Banerjee,Carl Meyer,Karina T Wright
Allogeneic chondrocyte therapies need to be developed to allow more individuals to be treated with a cell therapy for cartilage repair and to reduce the burden and cost of the current two-stage autologous procedures. Upscale manufacture of chondrocytes using a bioreactor could help provide an off-the-shelf allogeneic chondrocyte therapy with many doses being produced in a single manufacturing run.
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Three-Dimensional Printing Bioceramic Scaffolds Using Direct-Ink-Writing for Craniomaxillofacial Bone Regeneration. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-07-01 Vasudev Vivekanand Nayak,Blaire V Slavin,Edmara T P Bergamo,Andrea Torroni,Christopher M Runyan,Roberto L Flores,F Kurtis Kasper,Simon Young,Paulo G Coelho,Lukasz Witek
Defects characterized as large osseous voids in bone, in certain circumstances, are difficult to treat, requiring extensive treatments which lead to an increased financial burden, pain, and prolonged hospital stays. Grafts exist to aid in bone tissue regeneration (BTR), among which ceramic-based grafts have become increasingly popular due to their biocompatibility and resorbability. BTR using bioceramic
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TNF-α and OSX mRNA of Salivary Small Extracellular Vesicles in Periodontitis: A Pilot Study. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-07-01 Pingping Han,Kexin Jiao,Corey S Moran,Andrew Liaw,Yinghong Zhou,Carlos Salomon,Sašo Ivanovski
This cross-sectional pilot study explored extracellular vesicle (EV)-derived gene expression of markers for bone turnover and pro-inflammatory cytokines in periodontal disease. Whole unstimulated saliva was collected from 52 participants (18 healthy, 13 gingivitis, and 21 stages III/IV periodontitis), from which salivary small extracellular vesicles (sEVs) were enriched using the size-exclusion chromatography
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Effective Technical Protocol for Producing a Mono-Iodoacetate-Induced Temporomandibular Joint Osteoarthritis in a Rat Model. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-07-26 So-Yeon Yun,Yerin Kim,Hyunjeong Kim,Bu-Kyu Lee
An animal model of osteoarthritis (OA) induced by monosodium iodoacetate (MIA) can be effectively adjusted based on the concentration of MIA to control the onset, progression, and severity of OA as required. The rat temporomandibular joint osteoarthritis (TMJOA) model using MIA is a useful tool for studying the effectiveness of disease-modifying OA drugs in TMJOA research. However, the intricate and
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Regenerative Engineering of a Biphasic Patient-Fitted Temporomandibular Joint Condylar Prosthesis. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-07-01 David S Nedrelow,Ali Rassi,Boushra Ajeeb,Cameron P Jones,Pedro Huebner,Fabio G Ritto,Wendy R Williams,Kar-Ming Fung,Bradford W Gildon,Jakob M Townsend,Michael S Detamore
Regenerative medicine approaches to restore the mandibular condyle of the temporomandibular joint (TMJ) may fill an unmet patient need. In this study, a method to implant an acellular regenerative TMJ prosthesis was developed for orthotopic implantation in a pilot goat study. The scaffold incorporated a porous, polycaprolactone-hydroxyapatite (PCL-HAp, 20wt% HAp) 3D printed condyle with a cartilage-matrix-containing
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A Bibliometric Analysis of Research on Decellularized Matrix for Two Decades. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-07-07 Xueying Hou,Enchong Zhang,Yukun Mao,Jie Luan,Su Fu
The articles and reviews in the field of decellularized extracellular matrix (dECM) from 2001 to 2021 were retrieved and extracted from the Web of Science Core Collection. The R package Bibliometrix, CiteSpace, VOSviewer, and the online BIBLIOMETRC platform were utilized for bibliometric analysis, including specific characteristics of annual publications, influential countries/regions, core journals
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Osteogenic Activity on NaOH-Etched Three-Dimensional-Printed Poly-ɛ-Caprolactone Scaffolds in Perfusion or Spinner Flask Bioreactor. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-05-30 Hadi Seddiqi,Sonia Abbasi-Ravasjani,Alireza Saatchi,Ghassem Amoabediny,Behrouz Zandieh-Doulabi,Jianfeng Jin,Jenneke Klein-Nulend
Bioreactor systems, for example, spinner flask and perfusion bioreactors, and cell-seeded three-dimensional (3D)-printed scaffolds are used in bone tissue engineering strategies to stimulate cells and produce bone tissue suitable for implantation into the patient. The construction of functional and clinically relevant bone graft using cell-seeded 3D-printed scaffolds within bioreactor systems is still
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An Optimized Method for Microcomputed Tomography Analysis of Trabecular Parameters of Metal Scaffolds for Bone Ingrowth. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-06-01 Zhengmao Li,Qing Zhang,Shuang Yang,Yuncang Li,Andrej Atrens,Jagat Rakesh Kanwar,Wen Zhong,Bingpeng Lin,Cuie Wen,Yinghong Zhou,Yin Xiao
Owing to its superior mechanical and biological properties, titanium metal is widely used in dental implants, orthopedic devices, and bone regenerative materials. Advances in 3D printing technology have led to more and more metal-based scaffolds being used in orthopedic applications. Microcomputed tomography (μCT) is commonly applied to evaluate the newly formed bone tissues and scaffold integration
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Call for Papers: Special Issue on Modulation of the Immune System to Improve Tissue Regeneration Strategies. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-05-24 Laura Bracaglia,Themis Kyriakides
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Culture of Primary Neurons from Dissociated and Cryopreserved Mouse Trigeminal Ganglion. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-06-27 Molly Tzu-Yu Lin,Isabelle Xin Yu Lee,Wei-Li Chen,Mei-Yun Chen,Jodhbir S Mehta,Gary H F Yam,Gary S L Peh,Yu-Chi Liu
Corneal nerves originate from the ophthalmic branch of the trigeminal nerve, which enters the cornea at the limbus radially from all directions toward the central cornea. The cell bodies of the sensory neurons of trigeminal nerve are located in the trigeminal ganglion (TG), while the axons are extended into the three divisions, including ophthalmic branch that supplies corneal nerves. Study of primary
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Enhancement of Immunotherapies in Head and Neck Cancers Using Biomaterial-Based Treatment Strategies. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-06-01 Gemalene M Sunga,Jeffrey Hartgerink,Andrew G Sikora,Simon Young
Head and neck squamous cell carcinoma (HNSCC) is a challenging disease to treat because of typically late-stage diagnoses and tumor formation in difficult-to-treat areas, sensitive to aggressive or invasive treatments. To date, HNSCC treatments have been limited to surgery, radiotherapy, and chemotherapy, which may have significant morbidity and often lead to long-lasting side effects. The development
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Natural Polymer-Based Thin Film Strategies for Skin Regeneration in Lieu of Regenerative Dentistry. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-06-01 Parth Narayan Singh,Prasanna Kumar Byram,Lopamudra Das,Nishant Chakravorty
Wound healing (WH) is a complex and dynamic process that comprises of a series of molecular and cellular events that occur after tissue injury. The injuries of the maxillofacial and oral region caused by trauma or surgery result in undesirable WH such as delayed wound closure and formation of scar tissue. Skin tissue engineering (TE)/regeneration is an emerging approach toward faster, superior, and
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Editorial for Special Issue on Biomaterials and Cell Strategies for Regenerative Dentistry. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-05-01 Wei Ji,Yinghong Zhou,John A Jansen
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Advanced Methodology for Rapid Isolation of Single Myofibers from Flexor Digitorum Brevis Muscle. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-05-24 Kamal Awad,Logan Moore,Jian Huang,Lauren Gomez,Leticia Brotto,Venu Varanasi,Christopher Cardozo,Noah Weisleder,Zui Pan,Jingsong Zhou,Lynda Bonewald,Marco Brotto
Isolated individual myofibers are valuable experimental models that can be used in various conditions to understand skeletal muscle physiology and pathophysiology at the tissue and cellular level. This report details a time- and cost-effective method for isolation of single myofibers from the flexor digitorum brevis (FDB) muscle in both young and aged mice. The FDB muscle was chosen for its documented
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Lateral Bone Augmentation Using a Three-Dimensional-Printed Polymeric Chamber to Compare Biomaterials. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-05-24 Bart van Oirschot,Jeroen J J P van den Beucken,Antonios G Mikos,John A Jansen
The aim of this study was to test the suitability of calcium phosphate cement mixed with poly(lactic-co-glycolic acid) (CPC-PLGA) microparticles into a ring-shaped polymeric space-maintaining device as bone graft material for lateral bone augmentation. Therefore, the bone chambers were installed on the lateral portion of the anterior region of the mandibular body of mini-pigs. Chambers were filled
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Comparison of Osteogenic Capacity and Osteoinduction of Adipose Tissue-Derived Cell Populations. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-05-10 Johanna F A Husch,Laura Coquelin,Nathalie Chevallier,Dorien Tiemessen,Egbert Oosterwijk,René van Rheden,Charlotte Woud,Jurriaan Vossen,Sander C G Leeuwenburgh,Jeroen J J P van den Beucken
Stromal vascular fraction (SVF) is the primary isolate obtained after enzymatic digestion of adipose tissue that contains various cell types. Its successful application for cell-based construct preparation in an intra-operative setting for clinical bone augmentation and regeneration has been previously reported. However, the performance of SVF-based constructs compared with traditional ex vivo expanded
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Calcium Phosphate Ceramics and Synergistic Bioactive Agents for Osteogenesis in Implant Dentistry. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-05-01 Chunfeng Xu,Yuanyuan Sun,John Jansen,Menghong Li,Lingfei Wei,Yiqun Wu,Yuelian Liu
Implant-supported dental prosthetics are widely used in dental practice. Sufficient peri-implant bone tissue is a crucial prerequisite for the long-term success of this treatment, as insufficient peri-implant bone volume hampers dental implant installation and negatively influences dental implant stability. However, due to tooth extraction, bone metabolism diseases, and trauma, bone defects in the
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Bioactive Glasses in Periodontal Regeneration: A Systematic Review. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-05-02 Faleh Abushahba,Ahmed Algahawi,Nagat Areid,Leena Hupa,Timo O Närhi
Bioactive glasses (BAGs) are surface-active ceramic materials that can be used in bone regeneration due to their known osteoconductive and osteoinductive properties. This systematic review aimed to study the clinical and radiographic outcomes of using BAGs in periodontal regeneration. The selected studies were collected from PubMed and Web of Science databases, and included clinical studies investigating
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The Safety of Adiponectin Receptor Agonist AdipoRon in a Rabbit Model of Arthrofibrosis. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-04-01 Harold I Salmons,Christopher Gow,Afton K Limberg,Jacob W Bettencourt,Mason F Carstens,Ashley N Payne,Mark E Morrey,Joaquin Sanchez-Sotelo,Daniel J Berry,Amel Dudakovic,Matthew P Abdel
AdipoRon is an adiponectin receptor 1, 2 (ADIPOR1 and ADIPOR2) agonist with numerous reported physiological benefits in murine models of human disease, including a proposed reduction in fibrosis. However, AdipoRon has never been investigated in rabbits, which provide a robust model for orthopedic conditions. We examined the safety of intravenous (IV) AdipoRon in New Zealand White (NZW) female rabbits
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Differentiation of Human Induced Pluripotent Stem Cells into Mature and Myelinating Schwann Cells. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-03-16 Aurélie Louit,Marie-Josée Beaudet,Rémy Pépin,François Berthod
In the peripheral nervous system, Schwann cells (SCs) play a crucial role in axonal growth, metabolic support of neurons, and the production of myelin sheaths. Expansion of SCs after extraction from human or animal nerves is a long and often low-yielding process. We established a rapid cell culture method using a defined serum-free medium to differentiate human induced pluripotent stem cells (iPSCs)
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Cartilage Injury Repair by Human Umbilical Cord Wharton's Jelly/Hydrogel Combined with Chondrocyte. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-03-01 Jianhua Yang,Ying Zhao,Lei Fan,Cao Gao,Xuejian Liu,Xiaoguang Jing,Hongjun Zhang,Yong Huang,Rui Guo,Canling Long,Quanyi Guo,Jia Liu
Purpose: There is still a lack of effective treatments for cartilage damage. Cartilage tissue engineering could be a promising treatment method. Human umbilical cord Wharton's jelly (HUCWJ) and hydrogels have received wide attention as a scaffold for tissue engineering. They have not been widely used in clinical studies as their effectiveness and safety are still controversial. This study systematically
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Procedure of Adult Porcine Islet Isolation. Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-04-01 Naoaki Sakata,Gumpei Yoshimatsu,Ryo Kawakami,Kiyoshi Chinen,Chikao Aoyagi,Shohta Kodama
Islet transplantation is a useful therapeutic choice for severe diabetes mellitus; however, limited donor supplies have interfered with the use of this treatment. Therefore, the establishment of alternative donor sources and engineered tissue, which enables to produce appropriate insulin for controlling blood glucose, is an important challenge. The adult pig is a promising and feasible donor source
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Engineering High-Quality Cartilage Microtissues using Hydrocortisone Functionalised Microwells Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-01-31 Dr. Ross Burdis, Dr. Gabriela Soares Kronemberger, Dr. Daniel John Kelly
Engineering clinically relevant musculoskeletal tissues at a human scale is a considerable challenge. Developmentally inspired scaffold-free approaches for engineering cartilage tissues have shown great promise in recent years, enabling the generation of highly biomimetic tissues. Despite the relative success of these approaches, the absence of a supporting scaffold or hydrogel creates challenges in
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Construction of human three-dimensional lung model using layer-by-layer method Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-01-31 Ms. Yukako Akamatsu, Dr. Takami Akagi, Dr. Tomoko Sumitomo, Mr. Yuki Takahara, Dr. Shigehisa Akiyama, Prof. Shigetada Kawabata, Prof. Mitsuru Akashi
The respiratory tract is one of the frontline barriers for biological defense. Lung epithelial intercellular adhesions provide protection from bacterial and viral infections, and prevent invasion into deep tissues by pathogens. Dysfunction of lung epithelial intercellular adhesion caused by pathogens is associated with development of several diseases, such as acute respiratory distress syndrome (ARDS)
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Implanted tissue engineered vascular graft cell isolation with single-cell RNA sequencing analysis Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-01-31 Dr. Gabriel Jibril Mekiel Mirhaidari, Dr. Jenny Barker, Dr. Christopher K. Breuer, Mr. James Reinhardt
The advent of single-cell RNA sequencing (scRNA-Seq) has brought with it the ability to gain greater insights into the cellular composition of tissues and heterogeneity in gene expression within specific cell types. For tissue engineered blood vessels, this is particularly impactful to better understand how neotissue forms and remodels into tissue resembling a native vessel. A notable challenge, however
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Clinically relevant mesenchymal stem/stromal cell sheet transplantation method for kidney disease Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-01-31 Dr. Masatoshi Oka, Dr. Sumako Kameishi, Ms. Yun-Kyoung Cho, Prof. Sun U Song, Dr. David W Grainger, Prof. Teruo Okano
Chronic Kidney Disease (CKD) is the irreversible loss of nephron function, leading to a build-up of toxins, prolonged inflammation, and ultimately fibrosis. Currently, no effective therapies exist to treat CKD due to its complex pathophysiology. Mesenchymal stem/stromal cell (MSC) transplantation is a promising strategy to treat kidney diseases, and multiple clinical trials are currently ongoing. We
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Intratumor injection of thermosensitive polypeptide with resveratrol inhibits glioblastoma growth Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-01-31 Dr. Guoqing Yi, Dr. Huandong Liu, Dr. Fenglei Sun, Dr. Rong Du, Dr. Jun Kong, Dr. Hong Wang, Prof. Huilin Cheng, Prof. Guihuai Wang, Dr. Feng Gao, Dr. Ping Liang
Local tumor treatment is a feasible measure for patients with glioblastoma (GBM) who are unsuitable for surgical resection. Interferon-elastin-like polypeptide (IFN-ELP(V)) is a slow-release, biodegradable, thermosensitive fusion protein with antitumor immunity, and resveratrol (Res) is a polyphenolic compound with an antitumor effect. In this study, we found that intratumor injection of IFN-ELP(V)
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Optimization of FRESH Microspheres for Substantially Improved 3D Bioprinting Capabilities Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-01-31 Ms. Catherine A Wu, Dr. Yuanjia Zhu, Mr. Akshay Venkatesh, Mr. Charles J Stark, Ms. Seung Hyun Lee, Prof. Y Joseph Woo
Three-dimensional (3D) bioprinting demonstrates technology capable of producing structures comparable to native tissues in the human body. The Freeform Reversible Embedding of Suspended Hydrogels (FRESH) technique involves hydrogel-based bio-inks printed within a thermo-reversible support bath to provide mechanical strength to the printed construct. Smaller and more uniform microsphere sizes of FRESH
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Effect of pre-operative chemotherapy on the isolation outcome of primary human hepatocytes Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-01-24 Mr. Marco Rissel, Mr. Julian Pohl, Mr. Simon Moosburner, Mr. Joseph Maria George Vernon Gassner, Miss Rosa Horner, Mr. Karl Herbert Hillebrandt, Mr. Dominik Paul Modest, Prof. Johann Pratschke, Dr. Igor M. Sauer, Mr. Nathanael Raschzok
Primary human hepatocytes isolated from surgically resected liver tissue are an essential resource for pharmaceutical and toxicological studies. Patients undergoing partial liver resections have often received preoperative chemotherapy. The aim of our study was to investigate whether preoperative chemotherapy has effects on the outcome of cell isolation or the metabolic function of cultured hepatocytes
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Safety of Allogeneic Mesenchymal Stem Cell Seeding of NeuraGen® Nerve Guides in a Rabbit Model Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-01-21 Dr. Meiwand Bedar, Dr. Andre Van Wijnen, Dr. Alexander Y Shin
Mesenchymal stem cells (MSC) stimulate nerve and tissue regeneration and are primed for clinical translation. Application of autologous MSCs is limited by requirements for expedient harvesting procedures, proliferative expansion to increase cell numbers, and reduced regenerative potential due to aging or pathological conditions. Because MSCs are immune-privileged, allogeneic MSCs may serve as “off-the-shelf”
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The Development of Small-Caliber Vascular Grafts Using Human Umbilical Artery: An Evaluation of Methods Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-01-17 Vanessa Wong, Sheykha Gada, Melanie Singh, Nick Merna
Due to the prevalence of cardiovascular disease in the United States, small-caliber vascular grafts for coronary bypass surgery continue to be in high demand. Human umbilical arteries, an underutilized resource, were decellularized using zwitterionic (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate [CHAPS]) and ionic (sodium dodecyl sulfate [SDS]) detergents and evaluated as potential vascular
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An Acquired Anterior Glottic Web Model by Heat Injury with a Laryngoscopic Approach in a Rabbit Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-01-17 Juan-Juan Hu, Chen-Yu Zou, Rui Wang, Xiong-Xin Lei, Mao-Jia Chen, Ming Xiong, Yan-Lin Jiang, Jesse Li-Ling, Hui-Qi Xie, Hui Yang
Acquired anterior glottic webs (AGW) can lead to abnormally elevated phonatory pitch, dysphonia, and airway obstruction requiring urgent intervention. In this study, we construct a novel AGW rabbit model using heat injury by a laryngoscopic way. A primary study was conducted to identify the injury depth in rabbits' vocal folds (VFs) by graded heat energy, and the heat energy for the incurrence of epithelial
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Rodent Model for Orthotopic Implantation of Engineered Liver Devices Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-01-17 Tatevik Sahakyants, Tyler J. Lieberthal, Carly D. Comer, Matthew J. Hancock, Andrew P. Spann, Craig M. Neville, Joseph P. Vacanti
This study presents a novel surgical model developed to provide hematological support for implanted cellularized devices augmenting or replacing liver tissue function. Advances in bioengineering provide tools and materials to create living tissue replacements designed to restore that lost to disease, trauma, or congenital deformity. Such substitutes are often assembled and matured in vitro and need
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Enzymatic Isolation of Articular Chondrons: Is It Much Different Than That of Chondrocytes? Tissue Eng. Part C Method (IF 3.0) Pub Date : 2023-01-17 Marloes van Mourik, Gerke H. Schuiringa, Liesbeth P. Varion-Verhagen, Lucienne A. Vonk, Corrinus C. van Donkelaar, Keita Ito, Jasper Foolen
In native articular cartilage, chondrocytes (Chy) are completely capsulated by a pericellular matrix (PCM), together called the chondron (Chn). Due to its unique properties (w.r.t. territorial matrix) and importance in mechanotransduction, the PCM and Chn may be important in regenerative strategies. The current gold standard for the isolation of Chns from cartilage dates from 1997. Although previous
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An Improved Method for Assessing Macroscale Diffusion Through Osteonal Bone Tissue Eng. Part C Method (IF 3.0) Pub Date : 2022-12-13 Nicholas Cimino, Ronald J. Midura, Joanne M. Belovich
The characterization of diffusion through biological tissues has played an important role in fundamental medical research and product development. Understanding the diffusion phenomena allows for the identification of new concepts in fundamental science, evolving medical knowledge and improving future standards and protocols. To illustrate, the structure of cortical bone changes upon the onset of osteoporosis
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Detergent-Free Decellularization Preserves the Mechanical and Biological Integrity of Murine Tendon Tissue Eng. Part C Method (IF 3.0) Pub Date : 2022-12-13 Jason C. Marvin, Ai Mochida, Juan Paredes, Brenna Vaughn, Nelly Andarawis-Puri
Tissue decellularization has demonstrated widespread applications across numerous organ systems for tissue engineering and regenerative medicine applications. Decellularized tissues are expected to retain structural and/or compositional features of the natural extracellular matrix (ECM), enabling investigation of biochemical factors and cell-ECM interactions that drive tissue homeostasis, healing,