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CAP1, a target of miR‐144/451, negatively regulates erythroid differentiation and enucleation J. Cell. Mol. Med. (IF 4.486) Pub Date : 2021-01-26 Xiaoli Huang; Ruihua Chao; Yanyang Zhang; Pengxiang Wang; Xueping Gong; Dongli Liang; Yuan Wang
The exact molecular mechanism underlying erythroblast enucleation has been a fundamental biological question for decades. In this study, we found that miR‐144/451 critically regulated erythroid differentiation and enucleation. We further identified CAP1, a G‐actin‐binding protein, as a direct target of miR‐144/451 in these processes. During terminal erythropoiesis, CAP1 expression declines along with
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Human antigen R regulates hypoxia‐induced mitophagy in renal tubular cells through PARKIN/BNIP3L expressions J. Cell. Mol. Med. (IF 4.486) Pub Date : 2021-01-26 Shao‐Hua Yu; Kalaiselvi Palanisamy; Kuo‐Ting Sun; Xin Li; Yao‐Ming Wang; Feng‐Yen Lin; Kuen‐Bao Chen; I‐Kuan Wang; Tung‐Min Yu; Chi‐Yuan Li
Mitochondrial dysfunction contributes to the pathophysiology of acute kidney injury (AKI). Mitophagy selectively degrades damaged mitochondria and thereby regulates cellular homeostasis. RNA‐binding proteins (RBPs) regulate RNA processing at multiple levels and thereby control cellular function. In this study, we aimed to understand the role of human antigen R (HuR) in hypoxia‐induced mitophagy process
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GHR is involved in gastric cell growth and apoptosis via PI3K/AKT signalling J. Cell. Mol. Med. (IF 4.486) Pub Date : 2021-01-25 Hong‐Zhu Yan; Hua‐Feng Wang; Yueling Yin; Jue Zou; Feng Xiao; Li‐Na Yi; Ying He; Bo‐Sheng He
Growth hormone receptor (GHR), the cognate receptor of growth hormone (GH), is a membrane bound receptor that belongs to the class I cytokine receptor superfamily. GH binding GHR induces cell differentiation and maturation, initiates the anabolism inside the cells and promotes cell proliferation. Recently, GHR has been reported to be associated with various types of cancer. However, the underlying
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Non‐coding RNAs in cardiomyocyte proliferation and cardiac regeneration: Dissecting their therapeutic values J. Cell. Mol. Med. (IF 4.486) Pub Date : 2021-01-25 Xiaoxuan Dong; Xiuyun Dong; Feng Gao; Ning Liu; Tian Liang; Feng Zhang; Xuyang Fu; Linbin Pu; Jinghai Chen
Cardiovascular diseases are associated with high incidence and mortality, contribute to disability and place a heavy economic burden on countries worldwide. Stimulating endogenous cardiomyocyte proliferation and regeneration has been considering as a key to repair the injured heart caused by ischaemia. Emerging evidence has proved that non‐coding RNAs participate in cardiac proliferation and regeneration
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Predictive and prognostic significance of tumour subtype, SSTR1‐5 and e‐cadherin expression in a well‐defined cohort of patients with acromegaly J. Cell. Mol. Med. (IF 4.486) Pub Date : 2021-01-24 Jiri Soukup; Helena Hornychova; Monika Manethova; Kvetoslava Michalova; Ludmila Michnova; Lenka Popovska; Veronika Skarkova; Tomas Cesak; David Netuka; Ales Ryska; Jan Cap; Václav Hána; Václav Hána; Michal Kršek; Eva Dvořáková; Michal Krčma; Ivica Lazurova; Věra Olšovská; Karel Starý; Peter Vaňuga; Filip Gabalec
In somatotroph pituitary tumours, somatostatin analogue (SSA) therapy outcomes vary throughout the studies. We performed an analysis of cohort of patients with acromegaly from the Czech registry to identify new prognostic and predictive factors. Clinical data of patients were collected, and complex immunohistochemical assessment of tumour samples was performed (SSTR1‐5, dopamine D2 receptor, E‐cadherin
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Transgenic overexpression of ITGB6 in intestinal epithelial cells exacerbates dextran sulfate sodium‐induced colitis in mice J. Cell. Mol. Med. (IF 4.486) Pub Date : 2021-01-24 Haiyan Chen; Liubo Chen; Xin Wang; Xiaoxu Ge; Lifeng Sun; Zhanhuai Wang; Xiaoming Xu; Yongmao Song; Jing Chen; Qun Deng; Haiting Xie; Ting Chen; Yan Chen; Kefeng Ding; Jingjing Wu; Jian Wang
Integrins, as a large family of cell adhesion molecules, play a crucial role in maintaining intestinal homeostasis. In inflammatory bowel disease (IBD), homeostasis is disrupted. Integrin αvβ6, which is mainly regulated by the integrin β6 subunit gene (ITGB6), is a cell adhesion molecule that mediates cell‐cell and cell‐matrix interactions. However, the role of ITGB6 in the pathogenesis of IBD remains
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RNA methyltransferase METTL3 induces intrinsic resistance to gefitinib by combining with MET to regulate PI3K/AKT pathway in lung adenocarcinoma J. Cell. Mol. Med. (IF 4.486) Pub Date : 2021-01-24 Fangyan Gao; Qianqian Wang; Chang Zhang; Chen Zhang; Tianyu Qu; Jingya Zhang; Jifu Wei; Renhua Guo
Clinical research data show that gefitinib greatly improves the progression‐free survival of patients, so it is used in advanced non‐small cell lung cancer patients with EGFR mutation. However, some patients with EGFR sensitive mutations do not have good effects on initial gefitinib treatment, and this mechanism is rarely studied. METTL3, a part of N6‐adenosine‐methyltransferase, has been reported
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Targeting the TR4 nuclear receptor with antagonist bexarotene can suppress the proopiomelanocortin signalling in AtT‐20 cells J. Cell. Mol. Med. (IF 4.486) Pub Date : 2021-01-24 Liqun Xia; Danyang Shen; Youyun Zhang; Jieyang Lu; Mingchao Wang; Huan Wang; Yuanlei Chen; Dingwei Xue; Dajiang Xie; Gonghui Li
Drug options for the life‐threatening Cushing's disease are limited, and surgical resection or radiation therapy is not invariably effective. Testicular receptor 4 (TR4) has been identified as a novel drug target to treat Cushing's disease. We built the structure model of TR4 and searched the TR4 antagonist candidate via in silico virtual screening. Bexarotene was identified as an antagonist of TR4
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Application of convolutional neural network on early human embryo segmentation during in vitro fertilization J. Cell. Mol. Med. (IF 4.486) Pub Date : 2021-01-24 Mingpeng Zhao; Murong Xu; Hanhui Li; Odai Alqawasmeh; Jacqueline Pui Wah Chung; Tin Chiu Li; Tin‐Lap Lee; Patrick Ming‐Kuen Tang; David Yiu Leung Chan
Selection of the best quality embryo is the key for a faithful implantation in in vitro fertilization (IVF) practice. However, the process of evaluating numerous images captured by time‐lapse imaging (TLI) system is time‐consuming and some important features cannot be recognized by naked eyes. Convolutional neural network (CNN) is used in medical imaging yet in IVF. The study aims to apply CNN on day‐one
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KYNU, a novel potential target that underpins CD44‐promoted breast tumour cell invasion J. Cell. Mol. Med. (IF 4.486) Pub Date : 2021-01-24 Maryam Al‐Mansoob; Ishita Gupta; Radoslaw Stefan Rusyniak; Allal Ouhtit
Using a validated tetracycline‐off‐inducible CD44 expression system in mouse model, we have previously demonstrated that the hyaluronan (HA) receptor CD44 promotes breast cancer (BC) metastasis to the liver. To unravel the mechanisms that underpin CD44‐promoted BC cell invasion, RNA samples were isolated from two cell models: (a) a tetracycline (Tet)‐Off‐regulated expression system of the CD44s in
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LKB1IP promotes pathological cardiac hypertrophy by targeting PTEN/Akt signalling pathway J. Cell. Mol. Med. (IF 4.486) Pub Date : 2021-01-24 Mi Tian; Xiuxin Jiang; Xinyun Li; Jianmin Yang; Cheng Zhang; Wencheng Zhang
Pathological cardiac hypertrophy represents a leading cause of morbidity and mortality worldwide. Liver kinase B1 interacting protein 1 (LKB1IP) was identified as the binding protein of tumour suppressor LKB1. However, the role of LKB1IP in the development of pathological cardiac hypertrophy has not been explored. The aim of this study was to investigate the function of LKB1IP in cardiac hypertrophy
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Silencing circ‐USP1 protects the renal tubular from kidney injury induced by hypoxia via modulating miR‐194‐5p/DNMT3A axis in acute renal allografts J. Cell. Mol. Med. (IF 4.486) Pub Date : 2021-01-23 Tao Yao; Dongqing Zha; Ping Gao; Xiaoyan Wu
Recent studies indicate that circular RNAs are involved in dysregulation of kidney injury. Nevertheless, the underlying mechanisms remain largely unclear. Therefore, this study sought to investigate the role of circ‐USP1 in the pathogenesis of early renal allografts. Thirty‐two male C57BL/6J mice aged between 6 and 8 weeks were divided into the sham and allograft groups. Thereafter, the association
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CircCAMSAP1 promotes hepatocellular carcinoma progression through miR‐1294/GRAMD1A pathway J. Cell. Mol. Med. (IF 4.486) Pub Date : 2021-01-23 Zongjiang Luo; Libai Lu; Qianli Tang; Wang Wei; Pengyu Chen; Yichen Chen; Jian Pu; Jianchu Wang
Hepatocellular carcinoma (HCC) is one of the most common cancers with high prevalence and mortality, and it has brought huge economic and health burden for the world. It is urgent to found novel targets for HCC diagnosis and clinical intervention. Circular RNA (circRNA) has been reported to participate in many cancer progressions including HCC, suggesting that circRNA might paly essential role in HCC
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Analysis of changes in circular RNA expression and construction of ceRNA networks in human dilated cardiomyopathy J. Cell. Mol. Med. (IF 4.486) Pub Date : 2021-01-22 Zhenhao Lin; Yongchao Zhao; Fangjie Dai; Enyong Su; Fuhai Li; Yan Yan
Dilated cardiomyopathy (DCM) is a severe life‐threatening disease worldwide, and the underlying mechanisms remain unclear. Circular RNAs (circRNAs) have been reported to play important roles in various cardiovascular diseases and can function as competitive endogenous RNAs (ceRNAs). However, their role in human DCM has not been fully elucidated. In the present study, heart samples from DCM patients
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The phenotypic and functional study of tissue B cells in respiratory system provided important information for diseases and development of vaccines J. Cell. Mol. Med. (IF 4.486) Pub Date : 2021-01-22 Li Fan; Qiongli Wu; Shuangpeng Kang; Binyan Yang; Changyou Wu
The field of tissue‐resident B cells has received increasing attention, yet the feature of tissue B cells in respiratory system is unclear. Here, we first show that non‐circulating B cells obtained from nasal, trachea and lung tissues are numerically and phenotypically distinct from their circulating counterparts. Analysis of single cell transcriptome sequence identified multiple differentially expressed
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An increased burden of rare exonic variants in NRXN1 microdeletion carriers is likely to enhance the penetrance for autism spectrum disorder J. Cell. Mol. Med. (IF 4.486) Pub Date : 2021-01-21 Cinzia Cameli; Marta Viggiano; Magali J. Rochat; Alessandra Maresca; Leonardo Caporali; Claudio Fiorini; Flavia Palombo; Pamela Magini; Renée C. Duardo; Fabiola Ceroni; Maria C. Scaduto; Annio Posar; Marco Seri; Valerio Carelli; Paola Visconti; Elena Bacchelli; Elena Maestrini
Autism spectrum disorder (ASD) is characterized by a complex polygenic background, but with the unique feature of a subset of cases (~15%‐30%) presenting a rare large‐effect variant. However, clinical interpretation in these cases is often complicated by incomplete penetrance, variable expressivity and different neurodevelopmental trajectories. NRXN1 intragenic deletions represent the prototype of
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Retraction J. Cell. Mol. Med. (IF 4.486) Pub Date : 2021-01-21
Retraction: Gastrodin relieves inflammation injury induced by lipopolysaccharides in MRC‐5 cells by up‐regulation of miR‐103. Zhuona Xi, Yahong Qiao, Jifang Wang, Jifang Wang, Hongjian Su, Zhen Bao, Hongyan Li, Xiaoming Liao and Xiaolan Zhong. J Cell Mol Med. 2020; 24: 1451‐1459. The above article, published online on 25 November 2019 in Wiley Online Library (https://onlinelibrary.wiley.com/doi/full/10
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Obesity regulates miR‐467/HoxA10 axis on osteogenic differentiation and fracture healing by BMSC‐derived exosome LncRNA H19 J. Cell. Mol. Med. (IF 4.486) Pub Date : 2021-01-20 Yijun Wang; Wentao Chen; Liang Zhao; Yadong Li; Zhenyu Liu; Hua Gao; Xiaodong Bai; Baojun Wang
This study explored the therapeutic effect of bone marrow mesenchymal stem cell‐derived exosomes on the treatment of obesity‐induced fracture healing. Quantitative real‐time PCR was used to detect the expression of lncRNA H19, miR‐467 and Hoxa10 and combined with WB detection to detect osteogenic markers (RUNX2, OPN, OCN). Determine whether exosomes have entered BMSCs by immunofluorescence staining
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Neuregulin‐1 alleviate oxidative stress and mitigate inflammation by suppressing NOX4 and NLRP3/caspase‐1 in myocardial ischaemia‐reperfusion injury J. Cell. Mol. Med. (IF 4.486) Pub Date : 2021-01-20 Fuhua Wang; Huan Wang; Xuejing Liu; Haiyi Yu; Xiaomin Huang; Wei Huang; Guisong Wang
Neuregulin‐1 (NRG‐1) is reported to be cardioprotective through the extracellular‐regulated protein kinase (ERK) 1/2 pathway in myocardial ischaemia‐reperfusion injury (MIRI). NOX4‐induced ROS activated NLRP3 inflammasome and exacerbates MIRI. This study aims to investigate whether NRG‐1 can suppress NOX4 by ERK1/2 and consequently inhibit the NLRP3/caspase‐1 signal in MIRI. The myocardial infarct
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miR‐34a in extracellular vesicles from bone marrow mesenchymal stem cells reduces rheumatoid arthritis inflammation via the cyclin I/ATM/ATR/p53 axis J. Cell. Mol. Med. (IF 4.486) Pub Date : 2021-01-19 Huaiguo Wu; Xike Zhou; Xuedong Wang; Wei Cheng; Xinjia Hu; Yueping Wang; Bing Luo; Wenjun Huang; Juan Gu
Extracellular vesicles (Evs) participate in the development of rheumatoid arthritis (RA), but the mechanisms remain unclear. This study aimed to determine the mechanism by which microRNA‐34a (miR‐34a) contained in bone marrow mesenchymal stem cell (BM‐MSC)‐derived Evs functions in RA fibroblast‐like synoviocytes (RA‐FLSs). BM‐MSC‐derived Evs and an Evs inhibitor were extracted. A rat model of RA was
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Bisphenol A at a human exposed level can promote epithelial‐mesenchymal transition in papillary thyroid carcinoma harbouring BRAFV600E mutation J. Cell. Mol. Med. (IF 4.486) Pub Date : 2021-01-19 Liuli Li; Hao Li; Jun Zh.ang; Xin Gao; Hao Jin; Renqi Liu; Zhen Zhang; Xuan Zhang; Xichang Wang; Peng Qu; Yuejiao Zhao; Xiaobo Lu
Bisphenol A (BPA), a ubiquitous endocrine‐disrupting chemical, alters the function of endocrine system and enhances the susceptibility to tumorigenesis in several hormone‐dependent tumours as thyroid carcinoma. About 50% of papillary thyroid cancers (PTC), the most common type of thyroid malignancy, harbours the BRAFV600E mutation. This study aimed to investigate a potential combined effect of BPA
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PGC‐1α protects from myocardial ischaemia‐reperfusion injury by regulating mitonuclear communication J. Cell. Mol. Med. (IF 4.486) Pub Date : 2021-01-19 Yan‐Qing Li; Yan Jiao; Ya‐Nan Liu; Jia‐ying Fu; Lian‐Kun Sun; Jing Su
The recovery of blood supply after a period of myocardial ischaemia does not restore the heart function and instead results in a serious dysfunction called myocardial ischaemia‐reperfusion injury (IRI), which involves several complex pathophysiological processes. Mitochondria have a wide range of functions in maintaining the cellular energy supply, cell signalling and programmed cell death. When mitochondrial
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The tyrosine kinase v‐Src modifies cytotoxicities of anticancer drugs targeting cell division J. Cell. Mol. Med. (IF 4.486) Pub Date : 2021-01-19 Ryuzaburo Yuki; Mari Hagino; Sachi Ueno; Takahisa Kuga; Youhei Saito; Yasunori Fukumoto; Noritaka Yamaguchi; Naoto Yamaguchi; Yuji Nakayama
v‐Src oncogene causes cell transformation through its strong tyrosine kinase activity. We have revealed that v‐Src‐mediated cell transformation occurs at a low frequency and it is attributed to mitotic abnormalities‐mediated chromosome instability. v‐Src directly phosphorylates Tyr‐15 of cyclin‐dependent kinase 1 (CDK1), thereby causing mitotic slippage and reduction in Eg5 inhibitor cytotoxicity.
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Protein arginine methyltransferase 5 (PRMT5) activates WNT/β‐catenin signalling in breast cancer cells via epigenetic silencing of DKK1 and DKK3 J. Cell. Mol. Med. (IF 4.486) Pub Date : 2021-01-18 Harshita Shailesh; Kodappully S. Siveen; Saïd Sif
Protein arginine methyltransferase 5 (PRMT5) activity is dysregulated in many aggressive cancers and its enhanced levels are associated with increased tumour growth and survival. However, the role of PRMT5 in breast cancer remains underexplored. In this study, we show that PRMT5 is overexpressed in breast cancer cell lines, and that it promotes WNT/β‐CATENIN proliferative signalling through epigenetic
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Therapy for advanced cholangiocarcinoma: Current knowledge and future potential J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-04 Mingxun Wang; Ziyan Chen; Pengyi Guo; Yi Wang; Gang Chen
Cholangiocarcinoma (CCA) is a biliary epithelial tumour that can emerge at any point in the biliary tree. It is commonly classified based on its anatomical site of development into intrahepatic cholangiocarcinoma (ICC), perihilar cholangiocarcinoma (PCC) and distal cholangiocarcinoma (DCC), each of which is associated with varying patient demographics, molecular characteristics and treatment options
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Cellular senescence, a novel therapeutic target for mesenchymal stem cells in acute kidney injury J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-07 Lingfei Zhao; Chenxia Hu; Fei Han; Dajin Chen; Yanhong Ma; Junni Wang; Jianghua Chen
Cellular senescence is a widespread cellular programme that is characterized by permanent cell cycle arrest. Senescent cells adopt a changed secretory phenotype that can alter cellular function. For years, cellular senescence has been thought to be a protective factor against cancer; however, it is now recognized that it has a dual effect on individuals. Co‐ordinated activation of cellular senescence
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Toll‐like receptors (TLRs): An old family of immune receptors with a new face in cancer pathogenesis J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-18 Yazdan Mokhtari; Atieh Pourbagheri‐Sigaroodi; Parisa Zafari; Nader Bagheri; Seyed H. Ghaffari; Davood Bashash
In the dark path of tumorigenesis, the more carefully the cancer biology is studied, the more brilliant answers could be given to the countless questions about its orchestrating derivers. The identification of the correlation between Toll‐like receptors (TLRs) and different processes involved in carcinogenesis was one of the single points of blinding light highlighting the interconnection between the
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Procyanidin B2 improves endothelial progenitor cell function and promotes wound healing in diabetic mice via activating Nrf2 J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-11-20 Jiawei Fan; Hairong Liu; Jinwu Wang; Jiang Zeng; Yi Tan; Yashu Wang; Xiaoping Yu; Wenlian Li; Peijian Wang; Zheng Yang; Xiaozhen Dai
One of the major reasons for the delayed wound healing in diabetes is the dysfunction of endothelial progenitor cells (EPCs) induced by hyperglycaemia. Improvement of EPC function may be a potential strategy for accelerating wound healing in diabetes. Procyanidin B2 (PCB2) is one of the major components of procyanidins, which exhibits a variety of potent pharmacological activities. However, the effects
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Anti‐tumour effects of a dual cancer‐specific oncolytic adenovirus on Breast Cancer Stem cells J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-11 Wenjie Li; Yiquan Li; Yingli Cui; Shanzhi Li; Yilong Zhu; Chao Shang; Gaojie Song; Zirui Liu; Zhiru Xiu; Jianan Cong; Tingyu Li; Xiao Li; Lili Sun; Ningyi Jin
Apoptin can specifically kill cancer cells but has no toxicity to normal cells. Human telomerase reverse transcriptase (hTERT) can act as a tumour‐specific promoter by triggering the expression of certain genes in tumour cells. This study aims to investigate the inhibitory effects and to explore the inhibitory pathway of a dual cancer‐specific recombinant adenovirus (Ad‐apoptin‐hTERTp‐E1a, Ad‐VT) on
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Anti–coronavirus disease 2019 (COVID‐19) targets and mechanisms of puerarin J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-11-26 Xingyue Qin; Chen Huang; Ka Wu; Yu Li; Xiao Liang; Min Su; Rong Li
The present study aimed to uncover the pharmacological function and underlying mechanism of puerarin as a potential treatment for COVID‐19, using an in silico methodology, including network pharmacology and molecular docking. The pivotal targets of puerarin to treat COVID‐19 were identified and included the epidermal growth factor receptor (EGFR), tumour necrosis factor (TNF), tumour protein p53 (TP53)
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Evaluation of piggyBac‐mediated anti‐CD19 CAR‐T cells after ex vivo expansion with aAPCs or magnetic beads J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-11-22 Li‐Rong Yang; Lin Li; Ming‐Yao Meng; Wen‐Ju Wang; Song‐Lin Yang; Yi‐Yi Zhao; Run‐Qing Wang; Hui Gao; Wei‐Wei Tang; Yang Yang; Li‐Li Yang; Li‐Wei Liao; Zong‐Liu Hou
Adoptive immunotherapy is a new potential method of tumour therapy, among which anti‐CD19 chimeric antigen receptor T‐cell therapy (CAR‐T cell), is a typical treatment agent for haematological malignancies. Previous clinical trials showed that the quality and phenotype of CAR‐T cells expanded ex vivo would seriously affect the tumour treatment efficacy. Although magnetic beads are currently widely
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Extracellular vesicles‐derived miR‐150‐5p secreted by adipose‐derived mesenchymal stem cells inhibits CXCL1 expression to attenuate hepatic fibrosis J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-20 Zhiyong Du; Tianchong Wu; Linsen Liu; Biwei Luo; Cuifeng Wei
Hepatic fibrosis (HF) is involved in aggravated wound‐healing response as chronic liver injury. Extracellular vesicles (EVs) carrying microRNA (miR) have been reported as therapeutic targets for liver diseases. In this study, we set out to explore whether adipose‐derived mesenchymal stem cells (ADMSCs)‐derived EVs containing miR‐150‐5p affect the progression of HF. Carbon tetrachloride (CCl4) was firstly
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Long non‐coding RNA EWSAT1 promoted metastasis and actin cytoskeleton changes via miR‐24‐3p sponging in osteosarcoma J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-11-22 Dewei Shen; Yize Liu; Yuexin Liu; Tao Wang; Lin Yuan; Xuyang Huang; Yong Wang
Non‐coding RNAs are closely associated with tumorigenesis in multiple malignant tumours, including osteosarcoma (OS). Long non‐coding RNA Ewing sarcoma‐associated transcript 1 (EWSAT1) plays a role in metastasis, and actin cytoskeletal changes in OS remain unclear. In the current study, we showed that EWSAT1 expression was up‐regulated in OS and that an elevation in the EWSAT1 expression level was
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Linagliptin prevents left ventricular stiffening by reducing titin cleavage and hypophosphorylation J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-09 Ilona Cuijpers; Anna‐Pia Papageorgiou; Paolo Carai; Melissa Herwig; Andreas Mügge; Thomas Klein; Nazha Hamdani; Elizabeth A. V. Jones; Stephane Heymans
The metabolic syndrome (MetS) is an escalating problem worldwide, causing left ventricular stiffening, an early characteristic of diastolic dysfunction for which no treatment exists. As diastolic dysfunction and stiffening in MetS patients are associated with increased circulating dipeptidyl peptidase‐4 (DPP‐4) levels, we investigated whether the clinically approved DPP‐4 inhibitor linagliptin reduces
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Association between lncRNA‐H19 polymorphisms and hepatoblastoma risk in an ethic Chinese population J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-11-24 Tianbao Tan; Jiahao Li; Yang Wen; Yan Zou; Jiliang Yang; Jing Pan; Chao Hu; Yuxiao Yao; Jiao Zhang; Yijuan Xin; Suhong Li; Huimin Xia; Jing He; Tianyou Yang
H19 polymorphisms are associated with increased susceptibility to several cancers; however, their role in hepatoblastoma remains unclear. In this study, we investigated the association between three H19 polymorphisms (rs2839698 G>A, rs3024270 C>G, rs217727 G>A) and hepatoblastoma susceptibility in 213 hepatoblastoma patients. The rs2839698 and rs3024270 polymorphisms were associated with significantly
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Nucleolin regulates the proliferation of vascular smooth muscle cells in atherosclerotic via Aurora B J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-11-20 Hui Sun; Lingjin Huang; Pengfei Liang; Yuting Tang; Cheng Chen; Huan Chen; Xiaofang Lin; Zhengyang Luo; Ying Li; Bimei Jiang; Xianzhong Xiao
Vascular smooth muscle cells (VSMCs) play a significant role in atherosclerosis. As a multifunctional protein, nucleolin (NCL) is involved in many important physiological and pathological processes. In this study, we aimed to investigate the role of nucleolin in VSMCs proliferation and cell cycle. The expression of nucleolin increased in VSMCs of mice with aortas advanced plaques. With the left common
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Investigation of hub genes and immune status in heart transplant rejection using endomyocardial biopsies J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-11-23 Meng‐xi Xiu; Yuan‐meng Liu; Wen‐jun Wang
T cell‒mediated rejection (TCMR) and antibody‐mediated rejection (ABMR) are severe post‐transplantation complications for heart transplantation (HTx), whose molecular and immunological pathogenesis remains unclear. In the present study, the mRNA microarray data set GSE124897 containing 645 stable, 52 TCMR and 144 ABMR endomyocardial biopsies was obtained to screen for differentially expressed genes
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TGFB1 polymorphisms and TGF‐β1 plasma levels identify gastric adenocarcinoma patients with lower survival rate and disseminated disease J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-04 Ignacio Juarez; Alberto Gutierrez; Christian Vaquero‐Yuste; Elisa M. Molanes‐López; Adela López; Inmaculada Lasa; Remedios Gómez; José Manuel Martin‐Villa
TGF‐β1 is involved in tumour growth. Four TGFB1 SNPs and TGF‐β1 production by stimulated PBMC were determined in seventy‐eight gastric adenocarcinoma patients. In addition, TGF‐β1 levels were measured in the plasma of further thirty patients. rs1800471‐G/C genotype was prevalent in patients (20.7%) compared to controls (8.4%), as it also was the rs1800468 SNP‐G/A genotype in stage IV patients (20.7%)
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Combined inhibition of Ref‐1 and STAT3 leads to synergistic tumour inhibition in multiple cancers using 3D and in vivo tumour co‐culture models J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-03 Rachel A. Caston; Fenil Shah; Colton L. Starcher; Randall Wireman; Olivia Babb; Michelle Grimard; Jack McGeown; Lee Armstrong; Yan Tong; Roberto Pili; Joseph Rupert; Teresa A. Zimmers; Adily N. Elmi; Karen E. Pollok; Edward A. Motea; Mark R. Kelley; Melissa L. Fishel
With a plethora of molecularly targeted agents under investigation in cancer, a clear need exists to understand which pathways can be targeted simultaneously with multiple agents to elicit a maximal killing effect on the tumour. Combination therapy provides the most promise in difficult to treat cancers such as pancreatic. Ref‐1 is a multifunctional protein with a role in redox signalling that activates
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Gracillin shows potent efficacy against colorectal cancer through inhibiting the STAT3 pathway J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-01 Lehe Yang; Tianru Zhu; Hua Ye; Yili Shen; Zhiping Li; Luye Chen; Canwei Wang; Xia Chen; Haiyang Zhao; Youqun Xiang; Zhongxiang Xiao; Chengguang Zhao; Jifa Li; Wanle Hu
Colorectal cancer (CRC) accounts for about 10% of all annually diagnosed cancers and cancer‐related deaths worldwide. STAT3 plays a vital role in the occurrence and development of tumours. Gracillin has shown a significant antitumour activity in tumours, but its mechanism remains unknown. The human CRC cell lines HCT116, RKO, and SW480 and immunodeficient mice were used as models to study the effects
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Licochalcone A inhibits EGFR signalling and translationally suppresses survivin expression in human cancer cells J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-11-27 Feng Gao; Ming Li; Xinfang Yu; Wenbin Liu; Li Zhou; Wei Li
Dysfunction of epidermal growth factor receptor (EGFR) signalling plays a critical role in the oncogenesis of non–small‐cell lung cancer (NSCLC). Here, we reported the natural product, licochalcone A, exhibited a profound anti‐tumour efficacy through directly targeting EGFR signalling. Licochalcone A inhibited in vitro cell growth, colony formation and in vivo tumour growth of either wild‐type (WT)
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ADFP promotes cell proliferation in lung adenocarcinoma via Akt phosphorylation J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-11-29 Xia Meng; Ruiying Sun; Wei Wang; Na Zhang; Shiguang Cao; Boxuan Liu; Ping Fang; Shanshan Deng; Shuanying Yang
Previously, we identified differentially expressed proteins, including ADFP, between lung adenocarcinoma (LAC) tissue and paired normal bronchioloalveolar epithelium. In this study, we investigated the role of ADFP in LAC. ADFP levels in the serum of patients with lung cancer and benign diseases were measured by enzyme‐linked immunosorbent assays (ELISA). shRNA was used to knock‐down or overexpress
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Neddylation inhibitor MLN4924 has anti‐HBV activity via modulating the ERK‐HNF1α‐C/EBPα‐HNF4α axis J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-02 Mingjie Xie; Huiting Guo; Guohua Lou; Jiping Yao; Yanning Liu; Yi Sun; Zhenggang Yang; Min Zheng
Hepatitis B virus (HBV) infection is a major public health problem. The high levels of HBV DNA and HBsAg are positively associated with the development of secondary liver diseases, including hepatocellular carcinoma (HCC). Current treatment with nucleos(t)ide analogues mainly reduces viral DNA, but has minimal, if any, inhibitory effect on the viral antigen. Although IFN reduces both HBV DNA and HBsAg
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Loureirin B activates GLP‐1R and promotes insulin secretion in Ins‐1 cells J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-10 Yanting Ding; Sijing Xia; Han Zhang; Qin Chen; Bing Niu
Loureirin B (LB) is a natural product derived from Sanguis draconis, which has hypoglycaemic effects. In order to research the possible target of LB in the treatment of diabetes, molecular docking was used to simulate the interaction between LB and potential targets, and among them, glucagon‐like peptide‐1 receptor (GLP‐1R) had the optimal results. Further, spectroscopy and surface plasmon resonance
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Tgfb3 and Mmp13 regulated the initiation of liver fibrosis progression as dynamic network biomarkers J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-02 Jinsheng Guo; Weixin Liu; Zhiping Zeng; Jie Lin; Xingxin Zhang; Luonan Chen
Liver fibrogenesis is a complex scar‐forming process in the liver. We suggested that the liver first responded to chronic injuries with gradual changes, then reached the critical state and ultimately resulted in cirrhosis rapidly. This study aimed to identify the tipping point and key molecules driving liver fibrosis progression. Mice model of liver fibrosis was induced by thioacetamide (TAA), and
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Stiffness of photocrosslinkable gelatin hydrogel influences nucleus pulposus cell propertiesin vitro J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-02 Panpan Xu; Jingjing Guan; Yu Chen; Hui Xiao; Tianhao Yang; Hengheng Sun; Nan Wu; Changchun Zhang; Yingji Mao
A key early sign of degenerative disc disease (DDD) is the loss of nucleus pulposus (NP) cells (NPCs). Accordingly, NPC transplantation is a treatment strategy for intervertebral disc (IVD) degeneration. However, in advanced DDD, due to structural damage of the IVD and scaffold mechanical properties, the transplanted cells are less viable and secrete less extracellular matrix, and thus, are unable
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Analysis of differentially expressed genes in oral epithelial cells infected with Fusobacterium nucleatum for revealing genes associated with oral cancer J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-02 Shuwei Zhang; Chen Li; Zhiying Zhang; Yuchao Li; Qian Li; Fengxue Geng; Junchao Liu; Yaping Pan
Accumulating evidence links Fusobacterium nucleatum with tumorigenesis. Our previous study demonstrated that F. nucleatum infection can induce epithelial‐mesenchymal transition (EMT) in oral epithelial cells and elaborated a probable signal pathway involved in the induction of EMT. However, the comprehensive profiling and pathways of other candidate genes involved in F. nucleatum promoting malignant
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COPD patients with chronic bronchitis and higher sputum eosinophil counts show increased type‐2 and PDE4 gene expression in sputum J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-09 Dave Singh; Michele Bassi; Deborah Balzano; Germano Lucci; Aida Emirova; Marie Anna Nandeuil; Gera Jellema; Ebenezer K. Afolabi; Brian Leaker; Oliver Kornmann; Kai Michael Beeh; Henrik Watz; Mirco Govoni
Chronic obstructive pulmonary disease (COPD) patients with higher eosinophil counts are associated with increased clinical response to phosphodiesterase‐4‐inhibitors (PDE4i). However, the underlying inflammatory mechanisms associated with this increased response is not yet elucidated. This post hoc analysis focused on sputum gene expression in patients with chronic bronchitis who underwent 32‐day treatment
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Association of CHI3L1 gene variants with YKL‐40 levels and hypertension incidence: A population‐based nested case‐control study in China J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-06 Tian Xu; Xiaowei Zheng; Aili Wang; Zhirong Guo; Yonghong Zhang
YKL‐40 was reported to be associated with the risk of hypertension. Whether the variants of CHI3L1 gene were associated with both YKL‐40 levels and hypertension needs to be further elucidated. In a 1:1 matched case‐control study of 507 pairs with available YKL‐40 levels and DNA samples nested in a prospective cohort of Chinese subjects, the 15 tag single nucleotide polymorphisms (SNPs) of CHI3L1 gene
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EZH2 is a potential prognostic predictor of glioma J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-04 Yi‐nan Chen; Shi‐qiang Hou; Rui Jiang; Jun‐long Sun; Chuan‐dong Cheng; Zhong‐run Qian
The enhancer of zeste homologue 2 (EZH2) is a histone H3 lysine 27 methyltransferase that promotes tumorigenesis in a variety of human malignancies by altering the expression of tumour suppressor genes. To evaluate the prognostic value of EZH2 in glioma, we analysed gene expression data and corresponding clinicopathological information from the Chinese Glioma Genome Atlas, the Cancer Genome Atlas and
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Overexpression of c‐Fos reverses osteoprotegerin‐mediated suppression of osteoclastogenesis by increasing the Beclin1‐induced autophagy J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-04 Xishuai Tong; Miaomiao Chen; Ruilong Song; Hongyan Zhao; Jianchun Bian; Jianhong Gu; Zongping Liu
Osteoclastogenesis requires the involvement of transcription factors and degrading enzymes, and is regulated by upstream and downstream signalling. However, c‐Fos how regulates osteoclastogenesis through autophagy remain unclear. This study aimed to explore the role of c‐Fos during osteoprotegerin (OPG)‐mediated suppression of osteoclastogenesis. We found that the number of osteoclasts and the expression
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Dysregulated YY1/PRMT5 axis promotes the progression and metastasis of laryngeal cancer by targeting Hippo pathway J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-07 Nan Wang; Di Wu; Qian Long; Yue Yan; Xiaoqi Chen; Zheng Zhao; Honghong Yan; Xinrui Zhang; Meilan Xu; Wuguo Deng; Xuekui Liu
Metastases lead to high mortality in laryngeal cancer, but the regulation of its underlying mechanisms remains elusive. We identified Protein arginine methyltransferase 5 (PRMT5) was significantly up‐regulated in laryngeal cancer tissues, which predicts poor patient prognosis. Functional assays demonstrated that PRMT5 overexpression promoted the invasive capacity and lymph node metastasis in vitro
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Complement C5 activation promotes type 2 diabetic kidney disease via activating STAT3 pathway and disrupting the gut‐kidney axis J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-06 Ling Li; Tiantian Wei; Shuyun Liu; Chengshi Wang; Meng Zhao; Yanhuan Feng; Liang Ma; Yanrong Lu; Ping Fu; Jingping Liu
Diabetic kidney disease (DKD) is a severe DM complication. While complement C5 up‐regulation and gut dysbiosis are found in T2DM, their roles in DKD are unclear. Here, we investigated the effect of C5 on the gut microbiota during DKD development. Renal C5a/C5a receptor (C5aR) expression changes were measured in T2DM patients and db/db mice. Db/db mice were treated with a C5aR antagonist (C5aRA), and
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Naringin attenuates cisplatin‐ and aminoglycoside‐induced hair cell injury in the zebrafish lateral line via multiple pathways J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-03 Ming Li; Jingwen Liu; Dong Liu; Xuchu Duan; Qingchen Zhang; Dawei Wang; Qingyin Zheng; Xiaohui Bai; Zhiming Lu
Exposure to ototoxic drugs is a significant cause of hearing loss that affects about 30 thousand children with potentially serious physical, social and psychological dysfunctions every year. Cisplatin (CP) and aminoglycosides are effective antineoplastic or bactericidal drugs, and their application has a high probability of ototoxicity which results from the death of hair cells (HCs). Here, we describe
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MiR‐19b‐3p accelerates bone loss after spinal cord injury by suppressing osteogenesis via regulating PTEN/Akt/mTOR signalling J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-17 Da Liu; Bo Wang; Min Qiu; Ying Huang
Rapid and extensive bone loss, one of the skeletal complications after spinal cord injury (SCI) occurrence, drastically sacrifices the life quality of SCI patients. It has been demonstrated that microRNA (miRNA) dysfunction plays an important role in the initiation and development of bone loss post‐SCI. Nevertheless, the effect of miR‐19b‐3p on bone loss after SCI is unknown and the accurate mechanism
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Ubiquitin‐specific protease 2 regulates Ang Ⅱ–induced cardiac fibroblasts activation by up‐regulating cyclin D1 and stabilizing β‐catenin in vitro J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-12 Qiong Xu; Mingke Liu; Fangcheng Zhang; Xiaolin Liu; Sisi Ling; Xuke Chen; Jielei Gu; Wenchao Ou; Shiming Liu; Ningning Liu
Cardiac fibrosis, featuring abnormally elevated extracellular matrix accumulation, decreases tissue compliance, impairs cardiac function and accelerates heart failure. Mounting evidence suggests that the ubiquitin proteasome pathway is involved in cardiac fibrosis. In the present study, ubiquitin‐specific protease 2 (USP2) was identified as a novel therapeutic target in cardiac fibrosis. Indeed, USP2
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Salvianolate ameliorates oxidative stress and podocyte injury through modulation of NOX4 activity in db/db mice J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-17 Yiran Liang; Hong Liu; Yi Fang; Pan Lin; Zhihui Lu; Pan Zhang; Xiaoyan Jiao; Jie Teng; Xiaoqiang Ding; Yan Dai
Podocyte injury is associated with albuminuria and the progression of diabetic nephropathy (DN). NADPH oxidase 4 (NOX4) is the main source of reactive oxygen species (ROS) in the kidney and NOX4 is up‐regulated in podocytes in response to high glucose. In the present study, the effects of Salvianolate on DN and its underlying mechanisms were investigated in diabetic db/db mice and human podocytes.
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CD63 negatively regulates hepatocellular carcinoma development through suppression of inflammatory cytokine‐induced STAT3 activation J. Cell. Mol. Med. (IF 4.486) Pub Date : 2020-12-04 Shijun Yu; Jingde Chen; Ming Quan; Li Li; Yandong Li; Yong Gao
Tetraspanin CD63 has been widely implicated in tumour progression of human malignancies. However, its role in the tumorigenesis and metastasis of hepatocellular carcinoma (HCC) remains unclear yet. In the present study, we aimed to investigate the specific function and underlying mechanisms of CD63 in HCC progression. CD63 expression in HCC tissues was detected using immunohistochemistry and quantitative