-
Heterologous production of (-)-geosmin in Saccharomyces cerevisiae J. Biotechnol. (IF 4.1) Pub Date : 2024-03-11 Dheeraj Madhukar Mahajan, Pramod Shankar Kumbhar, Rishi Jain
(-)-Geosmin has high demand in perfumes and cosmetic products for its earthy congenial aroma. The current production of (-)-geosmin is either by distillation of sun-baked soil or by inefficient chemical synthesis because of the presence of multiple chiral centers. Fermentation processes are not viable as the titers of the based processes are low. This work presents an alternative route by the heterologous
-
In vitro synthesis of oryzamutaic acid H using recombinant L-lysine ε-dehydrogenase from Agrobacterium tumefaciens J. Biotechnol. (IF 4.1) Pub Date : 2024-03-11 Yuta Horie, Makoto Uchikata, Sachie Ueda, Gai Yamahira, Masaru Hashimoto, Kimitoshi Sakamoto, Hiroyuki Ito, Shigeki Hamada
Oryzamutaic acids, possessing a nitrogen-containing heterocyclic skeleton, have been isolated and identified from a rice mutant. Although oryzamutaic acids are expected to be functional ingredients, their functionality is difficult to evaluate, because of their wide variety and presence in trace amounts. Furthermore, how oryzamutaic acid is synthesized is unclear. Therefore, we developed a simple enzymatic
-
Bioethanol Production from Characterized Pre-treated Sugarcane Trash and Jatropha Agrowastes J. Biotechnol. (IF 4.1) Pub Date : 2024-03-08 Naglaa A. Elnagdy, Tamer I.M. Ragab, Mohamed A. Fadel, Mohamed A. Abou-Zeid, Mona A. Esawy
Low production costs and a potential feedstock supply make lignocellulosic ethanol (bioethanol) an important source of advanced biofuels. The physical and chemical preparation of this kind of lignocellulosic feedstock led to a high ethanol yield. In order to increase the yield of fermentable sugars, pretreatment is an essential process step that alters the lignocellulosic structure and improves its
-
Reviewing critical TRPM2 variants through a structure-function lens J. Biotechnol. (IF 4.1) Pub Date : 2024-03-03 Ádám V. Tóth, Ádám Bartók
The transient receptor potential melastatin 2 (TRPM2) channel plays a central role in connecting redox state with calcium signaling in living cells. This coupling makes TRPM2 essential for physiological functions such as pancreatic insulin secretion or cytokine production, but also allows it to contribute to pathological processes, including neuronal cell death or ischemia-reperfusion injury. Genetic
-
Engineering carboxylic acid reductases and unspecific peroxygenases for flavor and fragrance biosynthesis J. Biotechnol. (IF 4.1) Pub Date : 2024-02-28 Alejandra Agosto-Maldonado, Jiantao Guo, Wei Niu
Emerging consumer demand for safer, more sustainable flavors and fragrances has created new challenges for the industry. Enzymatic syntheses represent a promising green production route, but the broad application requires engineering advancements for expanded diversity, improved selectivity, and enhanced stability to be cost-competitive with current methods. This review discusses recent advances and
-
Understanding the mechanism of polyethyleneimine-mediated cell disintegration and protein extraction in E. coli: The role of floc network formation and PEI molecular weight J. Biotechnol. (IF 4.1) Pub Date : 2024-02-28 Alexander Jurjevec, Cécile Brocard, Gerald Striedner, Monika Cserjan-Puschmann, José L. Toca-Herrera, Rainer Hahn
Cell disintegration and protein extraction are crucial steps in downstream process development for biopharmaceuticals produced in . In this study, we explored the extraction mechanism of polyethyleneimine (PEI) at the cellular level and characterized the floc network that is formed upon PEI addition by Focused Beam Reflectance Measurement and Dispersion Analyzer. PEI disintegrates the cells by detachment
-
Sorption properties of pristine and functionalized magnetic carbon nanotubes in relation to double-stranded DNA J. Biotechnol. (IF 4.1) Pub Date : 2024-02-28 Yanina Rezvantseva, Dmitry Zybin, Anna Prostyakova, Vladimir Oleinikov, Dmitry Kapustin
Owing to improvement of the molecular diagnostic methods using purified preparations of nucleic acids (NAs), the development of effective methods providing the isolation of DNA is still relevant. The sorption properties of magnetic multi-walled carbon nanotubes (MWCNTs), oxidized MWCNTs and MWCNTs (pristine and oxidized) modified with polydiallyldimethylammonium chloride (pDADMAC) with respect to double
-
DNA digestion and formation of DNA-network structures with Holliday junction-resolving enzyme Hjc_15-6 in conjunction with polymerase reactions J. Biotechnol. (IF 4.1) Pub Date : 2024-02-24 Josefin Ahlqvist, Joanna Tymecka-Mulik, Katarzyna Burkiewicz, Reine Wallenberg, Andrius Jasilionis, Eva Nordberg Karlsson, Slawomir Dabrowski
The recently identified novel Holliday junction-resolving enzyme, termed Hjc_15-6, activity investigation results imply DNA cleavage by Hjc_15-6 in a manner that potentially enhances the molecular self-assembly that may be exploited for creating DNA-networks and nanostructures. The study also demonstrates DNA polymerase acting in combination with Hjc_15-6 capability to produce large amounts of DNA
-
Enhancement of the biological activity of hydroxytyrosol through its oxidation by laccase from Trametes versicolor J. Biotechnol. (IF 4.1) Pub Date : 2024-02-23 Alexandra V. Chatzikonstantinou, Myrto G. Bellou, Stamatia Spyrou, Angelos Papanikolaou, Yannis V. Simos, Dimitrios Peschos, Haralambos Stamatis
The laccase-catalyzed oxidation of hydroxytyrosol (HT) towards the formation of its bioactive oligomer derivatives was investigated. The biocatalytic oligomerization was catalyzed by laccase from in aqueous or various water-miscible organic solvents and deep eutectic solvent (DES)-based media. Mass Spectroscopy and Nuclear Magnetic Resonance were used for the characterization of the products. The solvent
-
Viral inactivation for pH-sensitive antibody formats such as multi-specific antibodies J. Biotechnol. (IF 4.1) Pub Date : 2024-02-23 Anaïs Duret, Lionel Duarte, Laure Cahuzac, Apolline Rondepierre, Monia Lambercier, Romain Mette, Achim Recktenwald, Roberto Giovannini, Martin Bertschinger
Recently developed multi-specific antibody formats enable new therapeutic concepts. Conveniently, formats with an Fc domain allow purification in well-established mAb platform processes. However, due to the structural complexity of the formats, the assembled molecules may be sensitive to extreme pH commonly used for viral inactivation. An alternative to low pH incubation for virus inactivation is the
-
Improved biocatalytic activity of steapsin lipase in supercritical carbon dioxide medium for the synthesis of benzyl butyrate: A commercially important flavour compound J. Biotechnol. (IF 4.1) Pub Date : 2024-02-23 Kirtikumar C. Badgujar, Jagruti K. Badgujar, Bhalchandra M. Bhanage
Enzymatic synthesis of flavours, fragrances and food additives compounds have great demand and market value. Benzyl butyrate is commercially important flavour and food additive compound having global use around 100 metric tons/year and widely used in various industrial sectors. However, industrial synthesis of food additive benzyl butyrate is carried out by conventional chemical process which demands
-
Saline extraction assisted by ultrasound: a method to obtain purified phycocyanin J. Biotechnol. (IF 4.1) Pub Date : 2024-02-22 Ingrid Rocha Teixeira, Ligia Damasceno Ferreira Marczak, Giovana Domeneghini Mercali, Débora Pez Jaeschke
-
Nitrile-synthesizing enzymes and biocatalytic synthesis of volatile nitrile compounds: A review J. Biotechnol. (IF 4.1) Pub Date : 2024-02-21 Takuya Yamaguchi, Yasuhisa Asano
Nitriles (R−CN) comprise a broad group of chemicals industrially produced and used in fine chemicals, pharmaceuticals, and bulk applications, polymer chemistry, solvents, etc. Nitriles are important starting materials for producing carboxylic acids, amides, amines, and several other compounds. In addition, some volatile nitriles have been evaluated for their potential as ingredients in fragrance and
-
Immobilization of aldoxime dehydratases on metal affinity resins and use of the immobilized catalysts for the synthesis of nitriles important in fragrance industry J. Biotechnol. (IF 4.1) Pub Date : 2024-02-17 Barbora Křístková, Ludmila Martínková, Lenka Rucká, Michael Kotik, Natalia Kulik, Robert Rädisch, Margit Winkler, Miroslav Pátek
Nitriles have a wide range of uses as building blocks, solvents, and alternative fuels, but also as intermediates and components of flavors and fragrances. The enzymatic synthesis of nitriles by aldoxime dehydratase (Oxd) is an emerging process with significant advantages over conventional approaches. Here we focus on the immobilization of His-tagged Oxds on metal affinity resins, an approach that
-
-
Integrative analysis of the transcriptome and metabolome reveals Bacillus atrophaeus WZYH01-mediated salt stress mechanism in maize (Zea mays L.) J. Biotechnol. (IF 4.1) Pub Date : 2024-02-10 Yaling Hou, Wenzhi Zeng, Chang Ao, Jiesheng Huang
Maize is an important food crop that is affected by salt stress during growth, which can hinder plant growth and result in a significant decrease in yield. The application of plant growth-promoting rhizobacteria can improve this situation to a certain extent. However, the gene network of rhizosphere-promoting bacteria regulating the response of maize to salt stress remains elusive. Here, we used metabolomics
-
Maduramycin, a novel glycosylation modulator for mammalian fed-batch and steady-state perfusion processes J. Biotechnol. (IF 4.1) Pub Date : 2024-02-09 Patrick Romann, Thomas Vuillemin, Silvia Pavone, Martin Jordan, Arnaud Perilleux, Jonathan Souquet, Jean-Marc Bielser, Christoph Herwig, Thomas K. Villiger
Controlling high-mannose (HM) content of therapeutic proteins during process intensification, reformulation for subcutaneous delivery, antibody-drug conjugate or biosimilar manufacturing represents an ongoing challenge. Even though a range of glycosylation levers to increase HM content exist, modulators specially increasing M5 glycans are still scarce. Several compounds of the polyether ionophore family
-
SWATH-MS as a strategy for CHO host cell protein identification and quantification supporting the characterization of mAb purification platforms J. Biotechnol. (IF 4.1) Pub Date : 2024-02-08 Sofia B. Carvalho, Ludivine Profit, Sushmitha Krishnan, Ricardo A. Gomes, Bruno M. Alexandre, Severine Clavier, Michael Hoffman, Kevin Brower, Patrícia Gomes-Alves
Host cell proteins (HCPs) are process-related impurities expressed by the host cells during biotherapeutics’ manufacturing, such as monoclonal antibodies (mAbs). Some challenging HCPs evade clearance during the downstream processing and can be co-purified with the molecule of interest, which may impact product stability, efficacy, and safety. Therefore, HCP content is a critical quality attribute to
-
RNA-based detection of genetically modified plants via current-voltage characteristic measurement J. Biotechnol. (IF 4.1) Pub Date : 2024-02-07 Chun-Kai Huang, Yi-Nan Lin, Wen-Shan Huang, Satyajyoti Senapati, Hsueh-Chia Chang, Yi-Ming Sun, Li-Fen Huang
The widespread adoption of genetically modified (GM) crops has escalated concerns about their safety and ethical implications, underscoring the need for efficient GM crop detection methods. Conventional detection methods, such as polymerase chain reaction, can be costly, lab-bound, and time-consuming. To overcome these challenges, we have developed RapiSense, a cost-effective, portable, and sensitive
-
Cytokines as fast indicator of infectious virus titer during process development J. Biotechnol. (IF 4.1) Pub Date : 2024-02-05 Johanna Bacher, Narges Lali, Florian Steiner, Alois Jungbauer
Measuring infectious titer is the most time-consuming method during the production and process development of live viruses. Conventionally, it is done by measuring the tissue culture infectious dose (TCID50) or plaque forming units (pfu) in cell-based assays. Such assays require a time span of more than a week to the readout and significantly slow down process development. In this study, we utilized
-
Construction and evaluation of a nanosystem that combines acidification promoted chemodynamic therapy and intracellular drug release monitoring J. Biotechnol. (IF 4.1) Pub Date : 2024-02-05 Kai Dong, Danyang Wang, Leiruo Lin, Peilin Niu, Yidong Wang, Qichao Tan, Jianfeng Xing
Triple-negative breast cancer (TNBC) is a highly invasive subtype of breast cancer that seriously affects women’s physical and mental health. Chemodynamic therapy (CDT) induces cell death by specifically generating Fenton/Fenton-like reactions within tumor cells. However, the weak acidity of the tumor microenvironment (TME) greatly weakens the effectiveness of CDT. This work constructed a kind of P-CAI/PT
-
OptMSP: A Toolbox for Designing Optimal Multi-Stage (Bio)Processes J. Biotechnol. (IF 4.1) Pub Date : 2024-02-05 Jasmin Bauer, Steffen Klamt
One central goal of bioprocess engineering is to maximize the production of specific chemicals using microbial cell factories. Many bioprocesses are one-stage (batch) processes (OSPs), in which growth and product synthesis are coupled. However, OSPs often exhibit low volumetric productivities due to the competition for substrate for biomass and product synthesis implying trade-offs between biomass
-
Interplay between desiccation and oxidative stress responses in iron-oxidizing acidophilic bacteria J. Biotechnol. (IF 4.1) Pub Date : 2024-02-03 Muñoz-Villagrán Claudia, Acevedo-Arbunic Javiera, Navarro-Salazar Sebastián, Fuentes-Rubio José, Levicán Gloria
Variations in water availability represent a foremost stress factor affecting the growth and survival of microorganisms. Acidophilic bioleaching bacteria are industrially applied for releasing metals from mineral sulphides, and they are considered extremely tolerant to oxidative conditions prevailing in acidic bioleaching environments. Such processes usually are performed in heaps and thus these microorganisms
-
One-pot process for the biotransformation of vegetable oils into natural deca- and dodecalactones J. Biotechnol. (IF 4.1) Pub Date : 2024-01-29 Stefano Serra, Davide De Simeis
-
The underlying mechanism of nuclear and mitochondrial DNA damages in triggering cancer incidences: Insights into proteomic and genomic sciences J. Biotechnol. (IF 4.1) Pub Date : 2024-02-02 Sami Saadi, Nor Elhouda Nacer, Nazamid Saari, Abdulkarim Sabo Mohammed, Farooq Anwar
The attempt of this review article is to determine the impact of nuclear and mitochondrial damages on the propagation of cancer incidences. This review has advanced our understanding to altered genes and their relevant cancerous proteins. The progressive raising effects of free reactive oxygen species ROS and toxicogenic compounds contributed to significant mutation in nuclear and mitochondrial DNA
-
Investigation of cell infiltration and colonization in 3D porous scaffolds via integrated experimental and computational strategies J. Biotechnol. (IF 4.1) Pub Date : 2024-02-01 Yu Xiang, Jiongyi Yan, Xujin Bao, Andrew Gleadall, Tao Sun
-
-
Predicting Drug Response of Small Cell Lung Cancer Cell Lines Based on Enrichment Analysis of Complex Gene Signatures J. Biotechnol. (IF 4.1) Pub Date : 2024-01-26 Kolos Nemes, Alexandra Benő, Petronella Topolcsányi, Éva Magó, Gabriella Mihalekné Fűr, Lőrinc S. Pongor
Advances in the field of genomics and transcriptomics have enabled researchers to identify gene signatures related to development and treatment of Small Cell Lung Cancer. In most cases, complex gene expression patterns are identified, comprising of genes with differential behavior. Most tools use single-genes as predictors of drug response, with only limited options for multi-gene use. Here we examine
-
Carrier-based immobilization of Aerococcus viridans l-lactate oxidase J. Biotechnol. (IF 4.1) Pub Date : 2024-01-26 Christiane Luley-Goedl, Margherita Bruni, Bernd Nidetzky
-
Cell-free reduction of carboxylic acids with secreted carboxylic acid reductase J. Biotechnol. (IF 4.1) Pub Date : 2024-01-22 Dominic Goj, Stella Ebner, Melissa Horvat, Simon Arhar, Ludmila Martínková, Margit Winkler
-
Identifying and charactering a 4-aminobutyryl-CoA ligase for the production of butyrolactam J. Biotechnol. (IF 4.1) Pub Date : 2024-01-19 Xiaolin Shen, Xiaotian Jiang, Xinxiao Sun, Qipeng Yuan, Jia Wang
-
pH-dependent electrostatic interactions between enzymes and nanoparticles in Pickering emulsions – Influence on activity and droplet size J. Biotechnol. (IF 4.1) Pub Date : 2024-01-19 Maximilian Seiler, Sebastian Stock, Anja Drews
Pickering emulsions (PE) are liquid-liquid systems that are stabilized by solid (nano)particles at the fluid interface. They offer higher stability, easier separation and lower toxicity compared to classical emulsions stabilized by surfactants. Common applications range from food science to drug delivery. In the last decade they have become of more interest in the field of multiphasic biocatalysis
-
Improvement in organic solvent resistance of keratinase BLk by directed evolution J. Biotechnol. (IF 4.1) Pub Date : 2024-01-18 Fucheng Zhu, Zixu Yan, Jingli Dai, Guosi Li, Qilin Xu, Yunfeng Ma, Jingbo Ma, Naidong Chen, Xinhong Zhang, Yongjun Zang
-
Volatile organic compounds: A threat to the environment and health hazards to living organisms – A review J. Biotechnol. (IF 4.1) Pub Date : 2024-01-17 Anju Mangotra, Shailesh Kumar Singh
-
-
Recent advances in DNA-based probes for photoacoustic imaging J. Biotechnol. (IF 4.1) Pub Date : 2024-01-09 Anusri Anil, Jyotsna Chaskar, Avinash B. Pawar, Abhishekh Tiwari, Atul Changdev Chaskar
-
Regulation of hydraulic retention time on caproic acid production via two-phase anaerobic fermentation of Chinese cabbage waste with autopoietic electron donors J. Biotechnol. (IF 4.1) Pub Date : 2024-01-02 Ranran Chen, Xiaofeng Ji, Zhengang Chen, Liu Huang, Jiying Zhu
The effects of hydraulic retention time (HRT) on the performance of two-phase anaerobic fermentation for caproic acid production from Chinese cabbage waste (CCW) were investigated. In the electron donor phase, yeast was inoculated to achieve efficient autopoietic ethanol, providing electron donors for the chain elongation process. Shorter HRT led to drastic fluctuations in microorganisms, thus resulting
-
Comparison of plant biostimulating properties of Chlorella sorokiniana biomass produced in batch and semi-continuous systems supplemented with pig manure or acetate J. Biotechnol. (IF 4.1) Pub Date : 2024-01-06 Wendy A. Stirk, Péter Bálint, Jitka Široká, Ondřej Novák, Tamás Rétfalvi, Zoltán Berzsenyi, Jácint Notterpek, Zoltán Varga, Gergely Maróti, Johannes van Staden, Miroslav Strnad, Vince Ördög
Microalgae-derived biostimulants provide an eco-friendly biotechnology for improving crop productivity. The strategy of circular economy includes reducing biomass production costs of new and robust microalgae strains grown in nutrient-rich wastewater and mixotrophic culture where media is enriched with organic carbon. In this study, Chlorella sorokiniana was grown in 100 l bioreactors under sub-optimal
-
Glycolate as alternative carbon source for Escherichia coli J. Biotechnol. (IF 4.1) Pub Date : 2024-01-06 Sonja Höhmann, Tim Arik Briol, Nadine Ihle, Oliver Frick, Andreas Schmid, Bruno Bühler
-
Enhanced catalytic activity and stability of lactate dehydrogenase for cascade catalysis of D-PLA by rational design J. Biotechnol. (IF 4.1) Pub Date : 2024-01-06 Yufeng Zhou, Xiaolong Sun, Jiahuan Hu, Yingjie Miao, Xiangyu Zi, Xi Luo, Yongqian Fu
Serving as a vital medical intermediate and an environmentally-friendly preservative, D-PLA exhibits substantial potential across various industries. In this report, the urgent need for efficient production motivated us to achieve the rational design of lactate dehydrogenase and enhance catalytic efficiency. Surprisingly, the enzymatic properties revealed that a mutant enzyme, LrLDHT247I/D249A/F306W/A214Y
-
Modification of the Loop Region Near the Substrate Tunnel to Alter the Hydrolytic Process of Dextranase J. Biotechnol. (IF 4.1) Pub Date : 2024-01-06 Bingbing Xia, Zhiwei Li, Jinao Wei, Ganpeng Hu, Jingwen Yang, Hongbin Zhang, Xueqin Hu
Dextranases are hydrolases that exclusively catalyze the disruption of α−1,6 glycosidic bonds. A series of variant enzymes were obtained by comparing the sequences of dextranases from different sources and introducing sequence substitutions. A correlation was found between the number of amino acids in the 397–401 region and the hydrolytic process. When there were no more than 5 amino acids in the 397–401
-
UV-ARTP compound mutagenesis breeding improves macrolactins production of Bacillus siamensis and reveals metabolism changes by proteomic J. Biotechnol. (IF 4.1) Pub Date : 2024-01-06 Lian Yu, Fei Li, Jie Ni, Xianling Qin, Junxiang Lai, Xinying Su, Zhe Li, Mengfei Zhang
Macrolactins are a type of compound with complex macrolide structure which mainly be obtained through microbiological fermentation now. They have excellent antifungal, antibacterial and antitumor activity. In order to improve macrolactins production, Bacillus siamensis YB304 was used as the research object, and a mutant Mut-K53 with stable genetic characters was selected by UV-ARTP compound mutagenesis
-
An insight into the nutritional and medicinal value of edible mushrooms: A natural treasury for human health J. Biotechnol. (IF 4.1) Pub Date : 2024-01-03 Arman Hamza, Ankit Mylarapu, K. Vijay Krishna, Devarai Santhosh Kumar
Edible mushrooms have been cherished worldwide because of their nutraceutical and medicinal properties. They are recognized as the new superfood for the future due to their low-calorie content, high-protein content, low lipid levels, low cholesterol levels, and abundance of essential vitamins. The fruiting body of edible mushrooms contains a plethora of primary and secondary metabolites. However, submerged
-
The CRISPR Cas patent files, part 2: Is Cpf1/Cas12a a less conflict- prone alternative to Cas9? J. Biotechnol. (IF 4.1) Pub Date : 2024-01-03 Ulrich Storz
CRISPR Cpf1/Cas12a has been discussed as a less conflict prone alternative, patent-wise, to Cas9. This article investigates whether or not this assumption is correct, and comes to the conclusion that the promise that CRISPR Cpf1/Cas12 would make things easier, and be less conflict-prone, is fragile.
-
Engineered praseodymium sulfide nanocarrier and supramolecular association of anticancer drug for effective delivery to breast cancer cells J. Biotechnol. (IF 4.1) Pub Date : 2024-01-03 Varnitha Manikantan, Govindaraj Sri Varalakshmi, Mano Magdalin Rubella Kennedy, Archana Sumohan Pillai, Aleyamma Alexander, Nikhil Mathew, Elackia Kathiravan, Israel V.M.V. Enoch
Metal sulfide nanoparticles are synthesized for their biomedical applications, including cancer drug targeting. This paper reports a novel nanocomposite made of praseodymium sulfide nanoparticles and poly-cyclodextrin. The praseodymium sulfide nanoparticles were synthesized hydrothermal, autoclaving the nitrate precursors at 150 °C for 18 hours. The material is characterized using XRD and shows an
-
Heterologous expression of the novel dimeric antimicrobial peptide LIG in Pichia pastoris J. Biotechnol. (IF 4.1) Pub Date : 2024-01-03 Lu Zhao, Ling Li, Mingyang Hu, Yuxin Fang, Na Dong, Anshan Shan
The antimicrobial peptide (AMP) LI is a fusion product of antimicrobial peptide LL37 produced by human neutrophils and Indolicidin secreted by bovine neutrophils. LI retained the antimicrobial activity of the parental peptides and showed high cell selectivity. In this study, the flexible linker Gly-Ser-Gly (G-S-G) was used to ligate LI into dimeric LIG, and constructed the Pichia pastoris (P. pastoris)
-
Production of functional recombinant roseltide rT1 antimicrobial peptide in tobacco plants J. Biotechnol. (IF 4.1) Pub Date : 2024-01-03 Amin Sahandi Khalifeh-Kandy, Fatemeh Dehghan Nayeri, Mohammad Ahmadabadi
Plant-derived peptides represent a promising group of natural compounds with broad industrial and pharmaceutical applications. Low-efficiency production level is the major obstacle to the commercial production of such bioactive peptides. Today, recombinant techniques have been developed for fast and cost-effective production of high-quality peptides for various applications in the chemical and food
-
Halophilic filamentous fungi and their enzymes: Potential biotechnological applications J. Biotechnol. (IF 4.1) Pub Date : 2023-12-28 Ines Ben Hmad, Ali Gargouri
Recently, interest in the study of microorganisms growing under extreme conditions, particularly halophiles, has increased due to their potential use in industrial processes. Halophiles are the class of microorganisms that grow optimally at high NaCl concentrations and are capable of producing halophilic enzymes capable of catalyzing reactions under harsh conditions. So far, fungi are the least studied
-
In silico designing and characterization of outer membrane protein K (OmpK) from Vibrio anguillarum and its expression in Nicotiana tabacum for the development of a plant-based vaccine against fish vibriosis J. Biotechnol. (IF 4.1) Pub Date : 2023-12-25 Muhammad Suleman Malik, Iqra Elahi, Muhammad Sameeullah, Fatima Ijaz, Neelam Batool, Fatima Khalid, Ekrem Gurel, Kiran Saba, Mohammad Tahir Waheed
Vibriosis is caused by Vibrio anguillarum in various species of aquaculture. A novel, secure, and stable vaccine is needed to eradicate vibriosis. Here, for reverse vaccinology and plant-based expression, the outer membrane protein K (OmpK) of V. anguillarum was chosen due to its conserved nature in all Vibrio species. OmpK, an ideal vaccine candidate against vibriosis, demonstrated immunogenic, non-allergic
-
Routine molecular applications and recent advances in breast cancer diagnostics J. Biotechnol. (IF 4.1) Pub Date : 2023-12-19 Gabriella Pankotai-Bodó, Orsolya Oláh-Németh, Farkas Sükösd, Tibor Pankotai
Cancer stands as one of the most common and lethal diseases, imposing a substantial burden on global mortality rates. Breast cancer is distinct from other forms of cancer in which it is the primary cause of death for women. Early detection of breast cancer can significantly lower the risk of mortality, improving the prognosis for those who are affected. The death rate of breast cancer has been steadily
-
Expression of a recombinant protein by an acetic acid bacterial host J. Biotechnol. (IF 4.1) Pub Date : 2023-12-21 Yuya Tanakura, Yasuyoshi Uekawa, Yuki Shige, Wakao Fukuda, Soichiro Ikuta, Heng Ning Wu, Kiyoshi Yasukawa, Itaru Yanagihara, Shinsuke Fujiwara
We evaluated the suitability of Komagataeibacter europaeus, a vinegar production organism adept at synthetic media growth, as a host for heterologous gene expression. Cryptic plasmids (pGE1 and pGE2 derivatives) from K. europaeus strain KGMA0119 were employed as vectors for heterologous gene expression. The focus was placed on the groES promoter as a potential inducible switch. The groES promoter was
-
Exploring the use of hexadecane by Yarrowia lipolytica: Effect of dissolved oxygen and medium supplementation J. Biotechnol. (IF 4.1) Pub Date : 2023-12-19 Sílvia M. Miranda, Marlene Lopes, Isabel Belo
This work aimed to evaluate the effect of medium composition and volumetric oxygen transfer coefficient (kLa) on Y. lipolytica growth and production of microbial lipids and enzymes from hexadecane. In the stirred tank bioreactor, increasing kLa from 11 h−1 to 132 h−1 improved the hexadecane assimilation rate, biomass concentration, and lipids synthesis (0.90 g·L−1). A cost-effective hexadecane-based
-
Adventitious root culture of Lessertia frutescens for the production of triterpenoid saponins and polysaccharides J. Biotechnol. (IF 4.1) Pub Date : 2023-12-15 Miao Wang, Mei-Yu Jin, Yi-Xuan Liu, Yu-Qing Guo, Han-Xi Li, Jun Jiang, Kee-Yeoup Peak, Xuan-Chun Piao, Mei-Lan Lian
Lessertia frutescens is a perennial shrub of commercial importance in South Africa, but the scarcity of plant resources has limited current product production. In this study, to provide an alternative approach for obtaining L. frutescens material, adventitious roots (ARs) were induced from sterilized seedlings and cultured in a suspension culture system. During this process, selection tests were conducted
-
POFUT1-mediated O-fucosylation of glycoproteins expressed in the baculovirus Sf9 insect cell expression system J. Biotechnol. (IF 4.1) Pub Date : 2023-12-07 Layla Haymour, Florian Pennarubia, Claire Colombel Le Faou, Emilie Pinault, Agnès Germot, Abderrahman Maftah, Sébastien Legardinier
-
Exploring RAD18-dependent replication of damaged DNA and discontinuities: A collection of advanced tools J. Biotechnol. (IF 4.1) Pub Date : 2023-12-10 Mónika Mórocz, Erda Qorri, Emese Pekker, Gabriella Tick, Lajos Haracska
DNA damage tolerance (DDT) pathways mitigate the effects of DNA damage during replication by rescuing the replication fork stalled at a DNA lesion or other barriers and also repair discontinuities left in the newly replicated DNA. From yeast to mammalian cells, RAD18-regulated translesion synthesis (TLS) and template switching (TS) represent the dominant pathways of DDT. Monoubiquitylation of the polymerase
-
Methyl oleate for plant protection products formulations: Enzymatic synthesis, reaction kinetics and application testing J. Biotechnol. (IF 4.1) Pub Date : 2023-12-09 Marta Martinez-Garcia, Wouter Van Hecke, Hilde Peeters, Dries Gabriels, Pieter Van der Weeën, Winnie Dejonghe, Yamini Satyawali
-
Targeting Long Non-Coding RNAs in Cancer Therapy using CRISPR-Cas9 Technology: A Novel Paradigm for Precision Oncology J. Biotechnol. (IF 4.1) Pub Date : 2023-12-07 Rahul Kumar Mahato, Srinjan Bhattacharya, Naina Khullar, Inderpal Singh Sidhu, P. Hemachandra Reddy, Gurjit Kaur Bhatti, Jasvinder Singh Bhatti
Cancer is the second leading cause of death worldwide, despite recent advances in its identification and management. To improve cancer patient diagnosis and care, it is necessary to identify new biomarkers and molecular targets. In recent years, long non-coding RNAs (lncRNAs) have surfaced as important contributors to various cellular activities, with growing proof indicating their substantial role
-
Catalytic and anti-cancer properties of platinum, gold, silver, and bimetallic Au-Ag nanoparticles synthesized by Bacillus sp. bacteria J. Biotechnol. (IF 4.1) Pub Date : 2023-12-03 Hamid Mollania, Majid Oloomi-buygi, Nasrin Mollania
Metallic nanoparticles play a significant role in the catalysis of chemical processes, besides, bimetallic nanoparticles with abundant active sites can reduce metallic nanoparticles toxicity in addition to increasing their catalytic performances. In this work, the platinum, gold, and silver nanoparticles are bio-synthesized using a native bacterium (GFCr-4). Also, the Au-Ag and Au@Ag bimetallic nanoparticles
-
Comprehensive evaluation of recombinant lactate dehydrogenase production from inclusion bodies J. Biotechnol. (IF 4.1) Pub Date : 2023-11-29 Chika Linda Igwe, Jan Niklas Pauk, Don Fabian Müller, Mira Jaeger, Dominik Deuschitz, Thomas Hartmann, Oliver Spadiut
A broad application spectrum ranging from clinical diagnostics to biosensors in a variety of sectors, makes the enzyme Lactate dehydrogenase (LDH) highly interesting for recombinant protein production. Expression of recombinant LDH is currently mainly carried out in uncontrolled shake-flask cultivations leading to protein that is mostly produced in its soluble form, however in rather low yields. Inclusion
-
Production of 1-methylxanthine via the biodegradation of theophylline by an optimized Escherichia coli strain J. Biotechnol. (IF 4.1) Pub Date : 2023-11-28 Meredith B. Mock, Shuyuan Zhang, Kayla Pakulski, Camden Hutchison, Margaret Kapperman, Tyler Dreischarf, Ryan M. Summers
1-Methylxanthine is a high-value derivative of caffeine of limited natural availability with many potential pharmaceutical applications. Unfortunately, production of 1-methylxanthine through purely chemical methods of synthesis are unfavorable due to lengthy chemical processes and the requirement of hazardous chemicals, ultimately resulting in low yields. Here, we describe a novel biosynthetic process