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Dogs are carriers of Clostridioides difficile lineages associated with human community-acquired infections Anaerobe (IF 2.709) Pub Date : 2021-01-06 Olivia Graaf Bjöersdorff; Sanna Lindberg; Kristoffer Kiil; Søren Persson; Luca Guardabassi; Peter Damborg
There is an increasing concern about the role of animals as reservoirs of Clostridioides difficile. In this study, we investigated prevalence, antimicrobial resistance and zoonotic potential of C. difficile in dogs. Two-hundred and twenty-five dog faecal deposits were collected from trashcans in nine public gardens. C. difficile was isolated using selective plating and enrichment culture, identified
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Non-oral Prevotella stepping into the spotlight Anaerobe (IF 2.709) Pub Date : 2021-01-19 Tomaž Accetto; Gorazd Avguštin
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Generating a fucose permease deletion mutant in Bifidobacterium longum subspecies infantis ATCC 15697 Anaerobe (IF 2.709) Pub Date : 2021-01-16 Melanie A. Higgins; Katherine S. Ryan
Bifidobacterium longum subsp. infantis ATCC 15697 has emerged as a model for infant gut-associated bifidobacterial strains. Here we present a genetic system for B. longum subsp. infantis ATCC 15697 using its own DNA restriction-modification systems and create a fucose permease deletion mutant lacking the ability to use free fucose as a carbon source.
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Does over a century of aerobic phage work provide a solid framework for the study of phages in the gut? Anaerobe (IF 2.709) Pub Date : 2021-01-16 Hiba Shareefdeen; Alexander P. Hynes
Bacterial viruses (bacteriophages, phages) of the gut have increasingly become a focus in microbiome studies, with an understanding that they are likely key players in health and disease. However, characterization of the virome remains largely based on bioinformatic approaches, with the impact of these viromes inferred based on a century of knowledge from aerobic phage work. Studying the phages infecting
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Extraintestinal Clostridioides difficile infection: Septic arthritis 12 months after colitis Anaerobe (IF 2.709) Pub Date : 2021-01-08 Suarez-Lopez Amaya; Escudero-Sánchez Rosa; García-Fernández Sergio; Alvarez Noelia; Rodríguez-Rojas Lourdes; Garagorri Eduardo; Ruiz Garbajosa Patricia; Cobo Javier
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Impact of the introduction of nucleic acid amplification testing on Clostridioides difficile detection and ribotype distribution in Wales Anaerobe (IF 2.709) Pub Date : 2020-12-09 Michael D. Perry; P. Lewis White; Trefor E. Morris
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In vitro sensitivity of 30 anaerobic bacterial strains of the human intestinal core microbiota to antibiotics: Culture and LC-MS/MS approaches Anaerobe (IF 2.709) Pub Date : 2020-12-30 Florine Ecale; Abdelaziz El Houari; Stéphanie Crapart; Jérome Laparre; Manilduth Ramnath; Jean-Marc Berjeaud; Marie-Hélène Rodier; Alexandre Crépin
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Rumen bacterial diversity in relation to nitrogen retention in beef cattle Anaerobe (IF 2.709) Pub Date : 2020-12-29 Kênia Larissa Gomes Carvalho Alves; Yury Tatiana Granja-Salcedo; Juliana Duarte Messana; Vinícius Carneiro de Souza; Maria Júlia Generoso Ganga; Paulo Henrique Detogni Colovate; Luciano Takeshi Kishi; Telma Teresinha Berchielli
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An uncommon case of native joint septic arthritis by Parvimonas micra Anaerobe (IF 2.709) Pub Date : 2020-12-26 Hassam Ali; Washma Amir; Nicole L. Bolick
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Development and application of aerobic, chemically defined media for Dysgonomonas Anaerobe (IF 2.709) Pub Date : 2020-12-01 Charles M. Bridges; Daniel J. Gage
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A novel case of bilateral tubo-ovarian abscesses attributed to Ruminococcus gnavus without gastrointestinal involvement Anaerobe (IF 2.709) Pub Date : 2020-11-30 Ruby Veale; Carly Hughes; Ian Woolley
Ruminococcus gnavus is a Gram-positive anaerobe and normal gut commensal in the human host. There have been a small number of reported cases of infections attributed to R. gnavus, and no cases of urogenital infections have previously been published. We describe here a case of bilateral tubo-ovarian abscesses (TOAs) which cultured a pure growth of R. gnavus in a young female with concurrent deep infiltrating
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Inhibitory effect of LL-37 and human lactoferricin on growth and biofilm formation of anaerobes associated with oral diseases Anaerobe (IF 2.709) Pub Date : 2020-11-26 Sabina Noreen Wuersching; Karin Christine Huth; Reinhard Hickel; Maximilian Kollmuss
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Toxinotyping and molecular characterization of antimicrobial resistance in Clostridium perfringens isolated from different sources of livestock and poultry Anaerobe (IF 2.709) Pub Date : 2020-11-19 Kottlahouse Anju; Kumaragurubaran Karthik; Vijayabalan Divya; Murugaiyan Latha Mala Priyadharshini; Rajeev Kumar Sharma; Seeralan Manoharan
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Anti-toxin antibody is not associated with recurrent Clostridium difficile infection Anaerobe (IF 2.709) Pub Date : 2020-11-21 Julie Gilbert; Jhansi Leslie; Rose Putler; Shayna Weiner; Alexandra Standke; Aline Penkevich; Micah Keidan; Vincent B. Young; Krishna Rao
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Carriage of Clostridioides difficile in healthy infants in the community of Handan, China: A 1-year follow-up study Anaerobe (IF 2.709) Pub Date : 2020-11-24 Qing-qing Cui; Jing Yang; Su-ju Sun; Zhi-rong Li; Cui-xin Qiang; Ya-nan Niu; Ru-xin Li; Dong-yan Shi; Hong-lian Wei; Tian-tian Tian; Kai-yue Xu; Wei-gang Wang; Jian-hong Zhao
Objective Clostridioides difficile may colonize healthy infants and young children asymptomatically and for the long-term. C. difficile genotypes and the rate and determinants of colonization differ substantially and vary among countries and regions. A 1-year follow-up study was performed to determine the incidence, kinetics and influencing factors of C. difficile intestinal colonization. Methods Twenty-nine
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Anaerobic bacterial communities associated with oral carcinoma: Intratumoral, surface-biofilm and salivary microbiota Anaerobe (IF 2.709) Pub Date : 2020-11-24 Janos Minarovits
It was estimated that more than 700 bacterial species inhabit the oral cavity of healthy humans. Anaerobes comprise a significant fraction of the oral bacteriome and play an important role in the formation of multi-species biofilms attached to various anatomical sites. Bacterial biofilms are also associated with pathologic laesions of the oral cavity, including oral squamous cell carcinoma (OSCC),
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A rare case of Clostridium paraputrificum bacteremia in a 78-year-old Caucasian man diagnosed with an intestinal neoplasm Anaerobe (IF 2.709) Pub Date : 2020-11-07 J. Intra; A. Milano; C. Sarto; P. Brambilla
Clostridium like species, particularly Clostridium perfrigens, are the second most common causes of human anaerobic infections, including myonecrosis and bacteremia. Clostridium paraputrificum is an infrequent isolate, which has been identified in only 1% of reported cases of clostridial infections. We herein report a rare case of C. paraputrificum bacteremia in a 78-year-old Caucasian man diagnosed
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Molecular characterization of pathogenicity locus (PaLoc) and tcdC genetic diversity among tcdA+B+ Clostridioides difficile clinical isolates in Tehran, Iran Anaerobe (IF 2.709) Pub Date : 2020-11-09 Mansoor Kodori; Zohreh Ghalavand; Abbas Yadegar; Gita Eslami; Masoumeh Azimirad; Marcela Krutova; Alireza Abadi; Mohammad Reza Zali
Clostridioides difficile is the main cause of healthcare-associated diarrhea worldwide. It is proposed that certain C. difficile toxinotypes with distinct pathogenicity locus (PaLoc) variants are associated with disease severity and outcomes. Additionally, few studies have described the common C. difficile toxinotypes, and also little is known about the tcdC variants in Iranian isolates. We characterized
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Flexible feeding in anaerobic digestion – Impact on process stability, performance and microbial community structures Anaerobe (IF 2.709) Pub Date : 2020-11-17 Nicolas Weithmann; Stanislava Mlinar; Eva Sonnleitner; Alfons Rupert Weig; Ruth Freitag
Biogas has the potential to contribute to some of the most urgent issues of the energy transition, including mobility, energy storage, and grid stability. Flexibilization has been discussed as a means to improve the economics of biogas production, ideally restricting the production of electricity to times of strong need. Here the possibility of demand-driven, flexible biogas production is investigated
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Septic arthritis post anterior cruciate ligament reconstruction due to Clostridioides difficile Anaerobe (IF 2.709) Pub Date : 2020-11-09 M.B. Eustace; A.V. Jennison; K.L. McCarthy
Clostridioides difficile is an obligate anaerobe ubiquitous in the environment and is of particular interest in the healthcare setting as a cause of healthcare associated infection usually presenting with colitis. Extracolonic manifestations of C. difficile infection are less common with only rare reports of septic arthritis primarily in the setting of relative or overt immunocompromise. This report
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A metagenomic assessment of microbial communities in anaerobic bioreactors and sediments: taxonomic and functional relationships Anaerobe (IF 2.709) Pub Date : 2020-11-15 Liov Karel Beraud-Martínez; Bruno Gómez-Gil; Miguel Ángel Franco-Nava; Pablo Almazán-Rueda; Miguel Betancourt-Lozano
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Genomic basis of antimicrobial resistance in non-toxigenic Clostridium difficile in Southeast Asia Anaerobe (IF 2.709) Pub Date : 2020-10-30 Korakrit Imwattana; Pattarachai Kiratisin; Thomas V. Riley; Daniel R. Knight
Despite being incapable of causing Clostridium difficile infection, non-toxigenic C. difficile (NTCD) may still be relevant. This study explored the role of NTCD as a reservoir of accessory antimicrobial resistance (AMR) genes in NTCD from Southeast Asia. This region has high rates of antimicrobial use, a high prevalence of NTCD and phenotypic AMR in such strains. More than half of the 28 NTCD strains
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Gas gangrene-associated gliding motility is regulated by the Clostridium perfringens CpAL/VirSR system Anaerobe (IF 2.709) Pub Date : 2020-10-29 Renzo G. Valeriani; LaMonta L. Beard; Abraham Moller; Kaori Ohtani; Jorge E. Vidal
Clostridium perfringens strains cause a wide variety of human and animal disease, including gas gangrene or myonecrosis. Production of toxins required for myonecrosis, PFO and CPA, is regulated by the C. perfringens Agr-like (CpAL) system via the VirSR two-component system. Myonecrosis begins at the site of infection from where bacteria migrate deep into the host tissue likely using a previously described
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In Vitro Fermentation and Isolation of Heparin-degrading Bacteria from Human Gut Microbiota Anaerobe (IF 2.709) Pub Date : 2020-11-01 Lin Pan; Weixia Sun; Qingsen Shang; Qingfeng Niu; Chanjuan Liu; Guoyun Li; Guangli Yu
Heparin and its derivative are commonly used as injectable anticoagulants in clinical, but with poor oral bioavailability. To explore the role of the gut microbiota in the poor oral effect of heparin, the degradation profiles of heparin by six human gut microbiota were investigated. The heparin-degradation ability varied significantly among individuals. Furthermore, two strains of heparin-degrading
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Analysis of a plasmid encoding botulinum neurotoxin type G gene in Clostridium argentinense Anaerobe (IF 2.709) Pub Date : 2020-10-12 Yoshihiko Sakaguchi; Jumpei Uchiyama; Akira Také; Kazuyoshi Gotoh; Masakiyo Sakaguchi; Tomonori Suzuki; Yumiko Yamamoto; Koji Hosomi; Tomoko Kohda; Masafumi Mukamoto; Shunji Kozaki; Shunji Hayashi; Keiji Oguma
Clostridium argentinense produces botulinum neurotoxin type G (BoNT/G). We sequenced and analyzed the plasmid harboring the bont/G gene, designated pCAG, in C. argentinense strain 2740. The pCAG consisted of 140,070 bp containing the bont/G gene cluster. Although this gene cluster showed high similarities in its DNA sequence and ORF arrangement to those of other bont gene clusters, the other regions
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Low prevalence of Cutibacterium acnes in prostatic tissue biopsies in a French hospital Anaerobe (IF 2.709) Pub Date : 2020-10-17 A.-L. Bidaud; G. Karam; C. Kandel-Aznar; L. Ruffier d’Epenoux; A. Guillouzouic; P. Bémer; A.-G. Leroy; S. Corvec
We evaluated the Cutibacterium acnes prevalence in prostatic biopsies and characterized the strains at a molecular level. 18 out of 36 biopsies (50%) were sterile after seven days in culture. C. acnes was observed in only two biopsies. Its prevalence was low (5.6%). Finally, the molecular characterization revealed diverse clusters including phylotypes IA1, IB and II.
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Prevalence of Clostridioides difficile associated diarrhea in hospitalized patients in five Brazilian centers: A multicenter, prospective study Anaerobe (IF 2.709) Pub Date : 2020-10-17 Evelyne Santana Girão; Bruno de Melo Tavares; Sania Alves dos Santos; Gessica Lorena Gamarra; Camila Rizek; Roberta Cristina Martins; Lauro Vieira Perdigão Neto; Constancia Diogo; Tatiana D’ Annibale Orsi; Evelyn Patricía Sanchez Espinoza; Hugo Manuel Paz Morales; Keite da Silva Nogueira; Adriane Ceshin Maestri; Icaro Boszczowski; Filipe Piastrelli; Cecilia Leite Costa; Daniely Viana Costa; Geovania
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Unravelling of nitric oxide signalling: A potential biomarker with multifaceted complex mechanism associated with canine inflammatory bowel disease (IBD) Anaerobe (IF 2.709) Pub Date : 2020-10-24 Tanmoy Rana
Inflammatory bowel disease (IBD) is an important chronic condition associated with the infection affecting the gastrointestinal tract (G.I.) in dogs. Several factors’ viz. gastrointestinal tract lymphoid tissue (GALT), permeability defects, genetic, ischemic, biochemical, psychosomatic disorders, infectious and parasitic agents, dietary allergies, and adverse drug reactions are associated with inflammatory
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Bacteremia caused by Veillonella dispar in an oncological patient Anaerobe (IF 2.709) Pub Date : 2020-10-16 Fernando Cobo; Virginia Pérez-Carrasco; José A. García-Salcedo; José María Navarro-Marí
Veillonella dispar is a Gram-negative anaerobic coccus involved in only a few human diseases. We report the second case of bacteremia due to this microorganism in an elderly patient. A 72-year-old man with a history of bladder cancer presented with diarrhea, vomiting, and fever for 48 hours. After the diagnosis of septic shock, four sets of blood cultures were taken, and three of them yielded V. dispar
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A rare case of polymicrobial chronic maxillary sinusitis due to concurrent isolation of Parvimonas micra and Fusobacterium nucleatum Anaerobe (IF 2.709) Pub Date : 2020-10-15 Theodoros Karampatakis; Anastasia Papavasiliou; Dimitris Tatsis; Konstantinos Paraskevopoulos; Helen Katsifa
Parvimonas micra and Fusobacterium nucleatum are commensal pathogens very rarely isolated simultaneously in clinical specimens. We report a case of chronic maxillary sinusitis caused by these two pathogens, presumably resulting from a co-existing dental infection.
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Characterization of medical relevant anaerobic microorganisms by isothermal microcalorimetry Anaerobe (IF 2.709) Pub Date : 2020-10-02 Stéphane Corvec; Eva Seiler; Lei Wang; Mercedes Gonzalez Moreno; Andrej Trampuz
Detection of anaerobe bacteria by culture methods requires appropriate media, special growth conditions, additional detection techniques and it typically takes several days. Therefore, anaerobes are often missed in patient specimens under routine culture conditions. Microcalorimetry may provide a simple and accurate real-time method for faster and better detection of anaerobes. An isothermal calorimeter
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C. difficile carriage in animals and the associated changes in the host fecal microbiota Anaerobe (IF 2.709) Pub Date : 2020-10-03 R. Thanissery; M.R. McLaren; A. Rivera; A.D. Reed; N.S. Betrapally; T. Burdette; J.A. Winston; M. Jacob; B.J. Callahan; C.M. Theriot
The relationship between the gut microbiota and Clostridioides difficile, and its role in the severity of C. difficile infection in humans is an area of active research. Intestinal carriage of toxigenic and non-toxigenic C. difficile strains, with and without clinical signs, is reported in animals, however few studies have looked at the risk factors associated with C. difficile carriage and the role
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First description of abdominal infection due to Alistipes onderdonkii Anaerobe (IF 2.709) Pub Date : 2020-10-03 Fernando Cobo; Carla Foronda; Virginia Pérez-Carrasco; Lina Martin-Hita; José A. García-Salcedo; José María Navarro-Marí
Alistipes spp is a genus of Gram-negative anaerobic rods involved in very few human diseases. We report the first case of abdominal infection due to Alistipes onderdonkii in a 58-year-old man with a history of chronic obstructive pulmonary disease. He presented with abdominal pain and general malaise after retrogastric drainage for a pancreatitis episode a few days earlier. After the diagnosis of diffuse
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Porphyromonas spp. have an extensive host range in ill and healthy individuals and an unexpected environmental distribution: A systematic review and meta-analysis Anaerobe (IF 2.709) Pub Date : 2020-10-01 Luis Acuña-Amador; Frédérique Barloy-Hubler
Studies on the anaerobic bacteria Porphyromonas, mainly focused on P. gingivalis, have revealed new bacterial structures, metabolic pathways, and physiologic functionalities. Porphyromonas are mainly described as being associated with mammals and involved in chronic oral infections and secondary pathologies such as cancers or neurodegenerative diseases. In this review, we collected and analyzed information
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Sneathia amnii, an unusual pathogen in spondylitis: A case report Anaerobe (IF 2.709) Pub Date : 2020-09-26 Claire Duployez; Rémi Le Guern; Emmanuel Faure; Frédéric Wallet; Caroline Loïez
Sneathia amnii is an opportunistic pathogen of the female reproductive tract that has been reported to cause infections during pregnancy and in the post-partum period. Infections outside the reproductive tract have rarely been described. We report the case of a spondylitis due to S. amnii in a 72-year old woman, successfully treated after seven weeks of antimicrobial therapy. Growth of this pathogen
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First case report of Solobacterium moorei bacteremia due to acute cholangitis in South Korea Anaerobe (IF 2.709) Pub Date : 2020-09-28 Jinho Lee; Seunghoo Lee; Do Hyun Park; Mi-Na Kim; Jiwon Jung
Solobacterium moorei is an anaerobic gram-positive bacillus that rarely causes bacteremia. Herein, we report a case of S. moorei bacteremia associated with acute cholangitis in a patient without malignancy. The patient had a history of chronic pancreatitis with pancreaticogastrostomy and presented with fever and abdominal pain. Computed tomography scans showed acute cholangitis and S. moorei identified
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Preferential use of plant glycans for growth by Bacteroides ovatus. Anaerobe (IF 2.709) Pub Date : 2020-09-12 Manuela Centanni,Tracey J Bell,Ian M Sims,Gerald W Tannock
B. ovatus is a member of the human gut microbiota with a broad capability to degrade complex glycans. Here we show that B. ovatus degrades plant polysaccharides in a preferential order, and that glycan structural complexity plays a role in determining the prioritisation of polysaccharide usage.
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Type 2 cytokines IL-4 and IL-5 reduce severe outcomes from Clostridiodes difficile infection Anaerobe (IF 2.709) Pub Date : 2020-09-22 Alexandra N. Donlan; Morgan E. Simpson; William A. Petri
Clostridiodes difficile infection (CDI) is the leading cause of hospital-acquired gastrointestinal infections in the U.S. While the immune response to C. difficile is not well understood, it has been shown that severe disease is accompanied by high levels of infiltrating immune cells and pro-inflammatory cytokine production. This study tests the roles of two type 2 cytokines, IL-4 and IL-5, in mediating
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Highbush blueberry proanthocyanidins alleviate Porphyromonas gingivalis-induced deleterious effects on oral mucosal cells. Anaerobe (IF 2.709) Pub Date : 2020-08-29 Amel Ben Lagha,Amy Howell,Daniel Grenier
Strong evidence points to Porphyromonas gingivalis, a Gram-negative anaerobic bacterium, as a keystone species in the development of the chronic form of periodontitis. The aim of the present study was to investigate the ability of highbush blueberry proanthocyanidins (PACs) to alleviate the P. gingivalis-induced deleterious effects on oral mucosal cells. We first showed that highbush blueberry PACs
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Physicochemical stressors and mixed alkaloid supplementation modulate ruminal microbiota and fermentation in vitro. Anaerobe (IF 2.709) Pub Date : 2020-08-28 Ratchaneewan Khiaosa-Ard,Mubarik Mahmood,Frederike Lerch,Franz-Pius Traintinger,Renée Maxine Petri,Matthias Münnich,Qendrim Zebeli
The drop of ruminal pH and heat are common physicochemical stressors challenging ruminal microbiota, nutrient digestion and cattle performance. We characterized the ruminal microbiota and digestive activity in response to different pH (6.0 and 6.6) and temperature (39.5 and 42 °C), as well as established the effective dose of alkaloid supplementation (0, 0.088 and 0.175% of feedstock DM) to modulate
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Uncommon isolation of Desulfovibrio vulgaris from a depressed fracture wound on the forehead. Anaerobe (IF 2.709) Pub Date : 2020-08-27 Akshatha R,Rakhi Biswas,Gopalakrishnan Madhavan Sasidharan,Sindhusuta Das
Desulfovibrio spp. are gram negative, obligate anaerobes capable of reducing sulfate. They have caused infections in humans, but very rarely. They are slow growers and difficult to identify. Hence, they are often overlooked and their actual presence goes unnoticed. Here, we describe a case of a 15- year old boy who was involved in a road traffic accident and he presented with seropurulent discharge
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Clostridium perfringens α-toxin inhibits myogenic differentiation of C2C12 myoblasts. Anaerobe (IF 2.709) Pub Date : 2020-08-26 Masaya Takehara,Keiko Kobayashi,Masahiro Nagahama
Clostridium perfringens type A is the causative agent of clostridial myonecrosis, and α-toxin has been reported to be responsible for the pathogenesis. Recently, it was reported that regeneration of skeletal muscle after C. perfringens-induced muscle disorders is delayed, but the detailed mechanisms have not been elucidated. Here, we tested whether α-toxin impairs the differentiation of C2C12 myoblasts
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An immunocompetent patient with culture-negative multiple brain abscesses caused by Fusobacterium nucleatum. Anaerobe (IF 2.709) Pub Date : 2020-08-22 Ariel Kenig,Asa Kessler,Shaheen Alaa,Wadia Hazu,Ayelet Michael-Gaygo,Sharon Amit,Efrat Orenbuch-Harroch
The diagnosis and treatment of brain abscesses have advanced due to the utilization of modern microbiological and neurosurgical methods. Here we present a 49-year-old female patient presented with headache and neurological symptoms. Initial evaluation revealed multiple ring-enhanced brain lesions and a lung cavitary lesion initially suspected to represent a malignant process. Stereotactic aspiration
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Clostridium perfringens α-toxin specifically induces endothelial cell death by promoting ceramide-mediated apoptosis. Anaerobe (IF 2.709) Pub Date : 2020-08-20 Masaya Takehara,Hiroto Bandou,Keiko Kobayashi,Masahiro Nagahama
Clostridium perfringens type A-induced gas gangrene is characterized by severe myonecrosis, and α-toxin has been revealed to be a major virulence factor involved in the pathogenesis. However, the detailed mechanism is unclear. Here, we show that CD31+ endothelial cell counts decrease in muscles infected with C. perfringens in an α-toxin-dependent manner. In vitro experiments revealed that α-toxin preferentially
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Association between the gut and oral microbiome with obesity. Anaerobe (IF 2.709) Pub Date : 2020-08-14 Asma Gasmi Benahmed,Amin Gasmi,Alexandru Doşa,Salvatore Chirumbolo,Pavan Kumar Mujawdiya,Jan Aaseth,Maryam Dadar,Geir Bjørklund
In recent decades, obesity has become one of the most common lifestyle-associated disorders. Obesity is a major contributing factor for several other lifestyles associated disorders such as type 2 diabetes mellitus, hypertension, and cardiovascular disease. Although genetics and lifestyle have been directly implicated in the onset and progression of obesity, recent studies have established that gut
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Phylum Synergistetes in the oral cavity: A possible contributor to periodontal disease. Anaerobe (IF 2.709) Pub Date : 2020-08-11 Barbara Anne McCracken,M Nathalia Garcia
Microbial contributions to periodontal disease have been under renewed scrutiny with the advent of newer technologies to identify their presence and gene expression at the molecular level. Members of the phylum Synergistetes are some of the more recent bacteria to be associated with periodontal disease. Bacteria classified in this phylum can be found in a wide variety of habitats including both inside
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An outbreak of Clostridioides difficile infections due to a 027-like PCR ribotype 181 in a rehabilitation centre: Epidemiological and microbiological characteristics. Anaerobe (IF 2.709) Pub Date : 2020-08-08 Melina Kachrimanidou,Amoe Baktash,Symeon Metallidis,Οlga Tsachouridou,Foteini Netsika,Despoina Dimoglou,Angeliki Kassomenaki,Eirini Mouza,Mina Haritonidou,Ed Kuijper
Clostridioides difficile is one of the most important healthcare-associated pathogens. Recently, several new 027-like types have been found that all belong to the multilocus sequence typing (MLST) Clade 2. We report a rapidly spreading outbreak of C. difficile infections (CDI) due to a newly identified PCR ribotype (RT) 181 in a Rehabilitation Centre (RC). Genomic analysis revealed the outbreak strain
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Phenotypic and genotypic antimicrobial resistance profiles of fecal lactobacilli from domesticated pigeons in Poland. Anaerobe (IF 2.709) Pub Date : 2020-08-08 Marta Dec,Dagmara Stępień-Pyśniak,Anna Nowaczek,Andrzej Puchalski,Renata Urban-Chmiel
Lactobacillus species play an important role in the host and although they are non-pathogenic, they could act as reservoirs for antibiotic resistance genes, with the potential risk of transfer to other bacteria inhabiting the gastrointestinal tract. The aim of this study was to identify Lactobacillus species derived from feces of domesticated pigeons and to characterize their phenotypic and genotypic
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Six cases of Solobacterium moorei isolated alone or in mixed culture in Hungary and comparison with previously published cases. Anaerobe (IF 2.709) Pub Date : 2020-08-07 Károly Péter Sárvári,Dóra Sántha,Réka Kovács,Sándor Körmöndi,Zoltán Pető,Tamás Vereb,Balázs Sztanó
Solobacterium moorei is a strict anaerobic gram-positive rod. It is found in the human microbiota in different parts of the body, but it also appears to be an opportunistic pathogen in some infectious processes. We describe six cases of severe infections identified in 2016 in which S. moorei was isolated alone or in mixed culture involving other anaerobes or both aerobic and anaerobic bacteria. Three
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FN3 protein fragment containing two type III fibronectin domains from B. longum GT15 binds to human tumor necrosis factor alpha in vitro. Anaerobe (IF 2.709) Pub Date : 2020-08-06 Ilya N Dyakov,Dilara A Mavletova,Irina N Chernyshova,Nadezda A Snegireva,Marina V Gavrilova,Kristina K Bushkova,Marina S Dyachkova,Maria G Alekseeva,Valery N Danilenko
Most species of the genus Bifidobacterium contain the gene cluster PFNA, which is presumably involved in the species-specific communication between bacteria and their hosts. The gene cluster PFNA consists of five genes including fn3, which codes for a protein containing two fibronectin type III domains. Each fibronectin domain contains sites similar to cytokine-binding sites of human receptors. Based
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Detection of beta-lactamase production in clinical Prevotella species by MALDI-TOF MS method. Anaerobe (IF 2.709) Pub Date : 2020-08-06 Nurver Ulger Toprak,Oncu Akgul,József Sóki,Guner Soyletir,Elisabeth Nagy,
Penicillins, can be used in treatment of infections due to Prevotella species if they are susceptible to penicillin. Early and accurate preliminary detection of β-lactamase-producing isolates is crucial for treatment of infection. The aim of this study was to determine β-lactamase-producing Prevotella species by MALDI-TOF MS and screen them for the presence of cfxA gene, responsible for β-lactamase
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Intracranial abscess due to Prevotella bivia: First case report from India. Anaerobe (IF 2.709) Pub Date : 2020-08-05 Subhashree Samantaray,Rakhi Biswas,Gopalakrishnan Madhavan Sasidharan
The association of Prevotella bivia (P. bivia), a Gram negative obligate anaerobic bacillus with brain abscess has been rarely reported. We hereby, report a case of brain abscess in a 50-year-old man, who suffered a head trauma followed by decompression surgery 10 months ago. Aspirated pus sample grew Methicillin resistant Staphylococcus aureus (MRSA) and P. bivia sensitive to metronidazole. The patient
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Paraclostridium dentum, a novel species with pathogenic features isolated from human dental plaque sample. Anaerobe (IF 2.709) Pub Date : 2020-08-03 Urvashi,Stanzin Choksket,Ashish Jain,Deepika Sharma,Vishakha Grover,Suresh Korpole
A strictly anaerobic bacterial strain designated as SKVG24 was isolated from subgingival dental plaque samples of patients suffering from periodontitis. Cells were stained Gram-positive, rod shaped with endospore. The strain showed negative reaction to catalase and oxidase enzymes, but positive for gelatinase activity. Optimal growth was observed at 37 °C temperature and 7.0 pH. The 16S rRNA gene sequence
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Human gut-derived commensal suppresses generation of T-cell response to gliadin in humanized mice by modulating gut microbiota. Anaerobe (IF 2.709) Pub Date : 2020-07-25 Rahul Bodkhe,Eric V Marietta,Baskar Balakrishnan,David H Luckey,Irina E Horwath,Yogesh S Shouche,Veena Taneja,Joseph A Murray
The human intestinal tract is colonized by a large number of diverse microorganisms that play various important physiologic functions. In inflammatory gut diseases including celiac disease (CeD), a dysbiotic state of microbiome has been observed. Interestingly, this perturbed microbiome is normalized towards eubiosis in patients showing recovery after treatment. The treatment has been observed to increase
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Clinical utility of anaerobic culture of cerebrospinal fluid. Anaerobe (IF 2.709) Pub Date : 2020-07-25 Kyriakos Chatzopoulos,Samantha Shannon,Audrey N Schuetz
Anaerobic meningitis is a rare serious clinical condition which mainly affects vulnerable populations and patients with predisposing factors such as head trauma, prior neurosurgical procedures or implantable medical devices such as ventriculoperitoneal shunts or ventricular drains. In this study we retrieved data from aerobic and anaerobic cultures of cerebrospinal (CSF) or ventricular fluid ordered
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Multicenter study of the epidemiology of Clostridioides difficile infection and recurrence in southern Brazil. Anaerobe (IF 2.709) Pub Date : 2020-07-24 Adriane Ceschin Maestri,Sonia Mara Raboni,Hugo Manuel Paz Morales,Leonardo Filipetto Ferrari,Felipe Francisco Bondan Tuon,Alexandre Losso,Camila Marconi,Keite da Silva Nogueira
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First description of bacteremia caused by Oscillibacter valericigenes in a patient hospitalized for leg amputation. Anaerobe (IF 2.709) Pub Date : 2020-07-23 Lauranne Broutin,Luc Deroche,Anthony Michaud,Gwenaël Le Moal,Christophe Burucoa,Louis-Etienne Gayet,Chloé Plouzeau,Maxime Pichon
Initially isolated from the alimentary canal of a Japanese corbicula clam, Oscillibacter valericigenes is a Gram-negative rod, of which culture remains very difficult. Herein we present the first case of bacteremia due to Oscillibacter valericigenes, in humans. A 55-year-old man was hospitalized for clinical management of multiple neglected leg wounds (colonized with maggots) that had occurred during
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Lactobacillus paraplantarum THG-G10 as a potential anti-acne agent with anti-bacterial and anti-inflammatory activities. Anaerobe (IF 2.709) Pub Date : 2020-07-23 HyeMin Cha,Su-Kyung Kim,MooChang Kook,Tae-Hoo Yi
Cutibacterium acnes (formerly Propionibacterium acnes) is the main bacterium targeted for the prevention and medical treatment of acne vulgaris. Lactic acid bacteria (LAB) are a group of microorganisms classified by their ability to produce lactic acid through fermentation. Although the activities of LAB have been studied, their potential anti-acne effects are not well known. Here, Lactobacillus paraplantarum
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Antimicrobial susceptibility patterns of clinically significant Gram-positive anaerobic bacteria in a Greek tertiary-care hospital, 2017-2019. Anaerobe (IF 2.709) Pub Date : 2020-07-22 Sofia Maraki,Viktoria Eirini Mavromanolaki,Dimitra Stafylaki,Anna Kasimati
Antimicrobial resistance among anaerobic bacteria is increasingly recognized with geographic differences. In this study we analyzed the distribution and antimicrobial susceptibility profiles of 358 Gram-positive clinically significant anaerobes, isolated from 2017 to 2019, in a Greek tertiary-care hospital. The species identification was performed by Vitek 2 and conventional biochemical methods, and
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Prevalence, molecular typing and antibiotic resistance of Clostridium perfringens in free range ducks in Northeast India. Anaerobe (IF 2.709) Pub Date : 2020-07-17 Arockiasamy Arun Prince Milton,Rajkumari Sanjukta,Akash Protim Gogoi,Kasanchi M Momin,Govindarajan Bhuvana Priya,Samir Das,Sandeep Ghatak,Arnab Sen,Basant Kumar Kandpal
This study reports faecal prevalence of Clostridium perfringens in free range ducks in North East India for the first time. We also report C. perfringens type A carrying cpb2 and cpe and type C carrying cpb2 and cpe strains in these ducks. Notably, a high prevalence (17.5%) of enterotoxin carrying C. perfringens strains and low antimicrobial resistance were observed.
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