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SELEX-discovered aptamer that inhibits cellular interleukin-17/interleukin-17 receptor interaction and antagonizes interleukin-17 signaling. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-11-29 Arifur Rahman Munshi,Tong Wang,Yukio Takamori,Takehiro Ando,Takumi Yokoyama,Daisuke Fuji,Zhehao Xu,Santhana Vedi,Mizuki Yamamoto,Keita Tsukamoto,Takashi Kawakami
This research is based on a systematic evolution of ligands by exponential enrichment (SELEX), also referred to as in vitro selection against the extracellular domain of human interleukin-17 receptor A (IL-17RA). Pull-down assay via quantitative polymerase chain reaction and chemiluminescence detection showed that the cloned RNA with the enriched sequence bound to human IL-17RA and inhibited the interaction
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Characterization of a novel L-fuconate dehydratase involved in the non-phosphorylated pathway of L-fucose metabolism from bacteria. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-11-28 Seiya Watanabe
A sugar acid dehydratase from Paraburkholderia mimosarum, potentially involved in the non-phosphorylated L-fucose pathway, was functionally characterized. A biochemical analysis revealed its unique heterodimeric structure and higher specificity towards L-fuconate than D-arabinonate, D-altronate, and L-xylonate, which differed from homomeric homologs. This unique L-fuconate dehydratase has a poor phylogenetic
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Exploration and utilization of novel aldoxime, nitrile, and nitro compounds metabolizing enzymes from plants and arthropods. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-11-28 Takuya Yamaguchi
Aldoxime (R1R2C = NOH) and nitrile (R - C≡N) are nitrogen-containing compounds that are found in species representing all kingdoms of life. The enzymes discovered from the microbial "aldoxime-nitrile" pathway (aldoxime dehydratase, nitrile hydratase, amidase, and nitrilase) have been thoroughly studied because of their industrial importance. Although plants utilize cytochrome P450 monooxygenases to
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Sake yeast symbiosis with lactic acid bacteria and alcoholic fermentation. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-11-24 Daisuke Watanabe
The yeast Saccharomyces cerevisiae plays a pivotal role in the production of fermented foods by converting sugars in ingredients into ethanol through alcoholic fermentation. However, how accurate is our understanding of its biological significance? Although yeast is essential to produce alcoholic beverages and bioethanol, yeast does not yield ethanol for humankind. Yeast obtains energy in the form
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Understanding the quality and safety of food production through the lens of The Microbiome of The Built Environment. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-11-22 Kota Imai,Ryo Niwa,Masaki Fujioka,Kohei Ito
The Microbiome of the Built Environment (MoBE) is profoundly implicated in various sectors, including food science. The balance between beneficial and pathogenic microbes in these facilities directly influences product quality and public health. Maintaining a careful check on MoBE and external microbes is vital to the food industry to ensure quality control. There is also a risk of contamination in
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Regulations and functions of proline utilization in yeast Saccharomyces cerevisiae. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-11-22 Akira Nishimura
The quality of alcoholic beverages strongly depends on the metabolic characteristics of the yeast cells being used. To control the aroma and taste of alcoholic beverages, as well as the production of ethanol in them, it is thus crucial to select yeast cells with the proper characteristics. Grape must contains a high concentration of proline, an amino acid that can potentially be a useful nitrogen source
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Intragastric administration of cinnamaldehyde induces changes in body temperature via TRPA1. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-11-22 Noriyuki Mori,Tomomi Urata
The transient receptor potential (TRP) channel family, including TRPA1, is known to be involved in temperature sensing and response. Previous studies have shown that intragastric administration of cinnamaldehyde (a typical TRPA1 agonist) can change body temperature, but the role of TRPA1 in this response is not clear. In this study, we found that intragastric administration of cinnamaldehyde increased
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Synthesis of isomaltooligosaccharides using 4-O-α-d-isomaltooligosaccharylmaltooligosaccharide 1,4-α-isomaltooligosaccharohydrolase. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-11-21 Noriaki Kitagawa,Hikaru Watanabe,Tetsuya Mori,Yuki Kawauchi,Hajime Aga,Shimpei Ushio,Koryu Yamamoto
Isomaltooligosaccharides (IMOs), including isomaltose, are valuable oligosaccharides, and the development of methods to synthesize high-purity IMOs has long been underway. We recently discovered a novel enzyme, 4-O-α-d-isomaltooligosaccharylmaltooligosaccharide 1,4-α-isomaltooligosaccharohydrolase (IMM-4IH), that showed promise for improving the synthesis process. In this study, we establish methods
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Long-term intake of α-glycerophosphocholine (GPC) suppresses microglial inflammation and blood-brain barrier (BBB) disruption and promotes neurogenesis in senescence-accelerated mice prone 8 (SAMP8). Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-11-21 Mitsuki Umino,Mayumi Okuda,Takeshi Ohkubo,Tsutomu Fujii,Kiminori Matsubara
We evaluated the effects of long-term glycerophosphocholine (GPC) intake on microglia, the blood-brain barrier (BBB), and neurogenesis in senescence-accelerated mice prone 8 (SAMP8). The GPC intake suppressed microglial activation and BBB disruption and sustained doublecortin-positive cells in the hippocampus. The results indicate that GPC intake exerts anti-inflammatory and neuroprotective effects
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Copper ion (Cu2+) is involved in the transcription of the tyrosinase-encoding melB gene of Aspergillus oryzae in solid-state culture. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-11-17 Jun Chen,Akio Tonouchi
In Aspergillus oryzae, the tyrosinase-encoding gene melB causes undesirable browning of sake and sake lees. This gene is known to be expressed specifically in solid-state culture; however, its expression mechanisms remain unknown. Here, we evaluated the possible factors affecting the transcription of melB and found that the copper ion (Cu2+) significantly enhanced the transcription level of melB in
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Architecture of Actinoplanes missouriensis sporangia and zoospores visualized using quick-freeze deep-etch electron microscopy. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-11-16 Shixuan Hu,Yuhei O Tahara,Takeaki Tezuka,Makoto Miyata,Yasuo Ohnishi
The architecture of sporangia and zoospores of Actinoplanes missouriensis was analyzed at a high resolution using quick-freeze deep-etch replica electron microscopy. This analysis revealed that (i) sporangia were surrounded by at least two membranous layers with smooth surfaces, (ii) zoospores were enclosed by a fibrillar layer, and (iii) flagella were generated in a restricted area on the zoospore
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Fed-batch cultivation of Euglena gracilis for the high-yield production and GPC-assisted molecular weight determination of paramylon. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-11-16 Mizuki Fukuda,Manato Kinkawa,Masahiro Hayashi
Paramylon, a starch-like carbohydrate accumulated in Euglena gracilis cells, could be a potential source as a raw material for chemical products; its high-yield production would thus be highly desired. Although the molecular weight and polymerization degree of paramylon are important properties for its use as a raw material for chemical products, the available information about paramylon molecular
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A convenient fluorimetry-based degranulation assay using RBL-2H3 cells. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-11-15 Hironori Higashio,Takuya Yokoyama,Tomoyuki Saino
Type I hypersensitivity is triggered by mast cell degranulation, a stimulus-induced exocytosis of preformed secretory granules containing various inflammatory mediators. The degree of degranulation is generally expressed as a percentage of secretory granule markers (such as β-hexosaminidase and histamine) released into the external solution, and considerable time and labor are required for the quantification
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Eugeniin improves cholesterol metabolism in HepG2 cells and Caco-2 cells. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-11-10 Yuyang Ye,Asahi Takeuchi,Yuya Kawaguchi,Shoya Matsuba,Ni Zhang,Maihemuti Mijiti,Arata Banno,Naoto Hiramatsu,Toshitaka Okada,Satoshi Nagaoka
Considering the absence of prior studies on the cholesterol metabolism-improving effects of eugeniin, the present investigation aimed to explore the potential impact of eugeniin on cholesterol metabolism. This study sought, to elucidate the molecular mechanisms involved in this process using HepG2 and Caco-2 cells treated with 5 µM eugeniin. The intracellular cholesterol levels in HepG2 and Caco-2
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The nucleolar protein NIFK accelerates the progression of colorectal cancer via activating MYC pathway. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-11-09 Tingting Xia,Bin Wang,Lingling Sun
This study aimed to explore the function of NIFK on colorectal cancer (CRC) and its associated molecular mechanisms. NIFK was upregulated in CRC tissues and cells. NIFK silencing resulted in reduced cell growth and metastasis, as well as in promoted apoptosis in CRC cells. Moreover, NIFK silencing was also confirmed to inhibit lipid accumulation and decrease fatty acid synthesis via downregulating
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Mouse TMC4 is involved in the detection of chloride taste of salts. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-11-08 Masataka Narukawa,Aya Masago,Momo Murata,Yoshikazu Saito,Yoichi Kasahara,Keiko Abe,Tomiko Asakura
Licking behavior with various salts in Tmc4 knockout (KO) mice was observed. In Tmc4 KO mice, a significant decrease in sensitivity to chloride salts, such as NaCl, KCl, and NH4Cl, was observed, while no significant decrease in sensitivity to Na gluconate was observed. This finding suggests that TMC4 may be involved in the detection of chloride taste.
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Independent, cooperative regulation of cellulolytic genes by paralogous transcription factors ClbR and ClbR2 in Aspergillus aculeatus. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-11-08 Emi Kunitake,Takashi Kawaguchi,Shuji Tani
The cellobiose-responsive regulator ClbR, a Zn(II)2Cys6 binuclear-cluster transcription factor, is a positive regulator of carbohydrate-active enzyme (CAZyme) genes responsive to cellulose in Aspergillus aculeatus. Because Zn(II)2Cys6 transcription factors tend to dimerize with proteins of the same family, we searched for a counterpart of ClbR and identified ClbR2, which is 42% identical to ClbR, as
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Procyanidins and sensory nutrition; Do procyanidins modulate homeostasis via astringent taste receptors? Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-11-01 Naomi Osakabe,Taiki Fushimi,Yasuyuki Fujii,Vittorio Calabrese
Long-term intake of procyanidins has been suggested to reduce the risk of cardiovascular disease, dementia, and sensory function decline associated with aging. However, most of the ingested procyanidins are not absorbed and are excreted in the feces, so the mechanism of their beneficial impact is unknown. Procyanidins are the components of astringency in plant foods and their stimulation appears to
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This review was written in response to the author's receipt of the JSBBA Award for Young Scientists in 2022. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-25 Yoshiya Seto
Strigolactones (SLs) are a class of terpenoid lactones initially identified as seed germination stimulants for root parasitic plants more than 50 years ago. Long after this initial discovery, SLs were re-characterized as the symbiotic signals for arbuscular mycorrhizal fungi that supply inorganic nutrients, such as phosphate, to their host plants. In 2008, SLs were found to be endogenous plant hormones
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α-Tocotrienol in rice bran enhances steroidogenesis in mouse Leydig cell via increased gene expression of steroidogenic acute regulatory protein and induction of its mitochondrial translocation. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-25 Naofumi Terada,Tomoaki Nagase,Hiromi Kayoka,Yusuke Adachi,Eisuke Kato
Rice is a staple food in the Asian region and one of the world's major energy sources. Testosterone is a steroid hormone that maintains physical, sexual, and cognitive ability and its decline causes health problems like late-onset hypogonadism. Evaluation of various grain extracts showed rice bran to stimulate testosterone secretion from Leydig model cell. α-Tocotrienol was found as a bioactive compound
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Salmon nasal cartilage proteoglycan stimulates hair growth. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-25 Akio Nakane,Shouhei Hirose,Noriaki Kawai,Naoki Fujimoto,Eriko Kondo,Krisana Asano
Hair loss is a commonly encountered problem. In this study, hair growth was enhanced by daily oral ingestion of salmon nasal cartilage proteoglycan (PG) in mice. PG stimulated VEGF production in human follicle dermal papilla cells through IGF-1 receptor signaling, suggesting the possibility of hair loss improvement by PG ingestion.
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Interleukin-21 knockout reduces bone loss in ovariectomized mice by inhibiting osteoclastogenesis. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-25 Junlong Hou,Ping Xu,Yanheng Zhong,Zhigang Zhou,Wencai Zhang
Estrogen deficiency accelerates osteoporosis in elderly women. However, the role of IL-21 in postmenopausal osteoporosis remains unclear. Female wild-type (WT) C57BL/6 and IL-21 knockout (KO) mice were used for ovariectomy (OVX). Here, IL-21 levels were significantly increased in the serum and bone tissues of WT-OVX mice. The trabecular bone space of the femur was significantly increased, and the bone
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Enhanced intestinal barrier function as the mechanism of antibiotic growth promoters in feed additives. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-25 Akinori Uehara,Mayumi Maekawa,Kazuki Nakagawa
Antibiotic growth promoters (AGPs) are a cost-effective tool for improving livestock productivity. However, antimicrobial-resistant bacteria have emerged, and the search for alternatives to AGPs has consequently intensified. To identify these alternatives without the risk of the emergence of antimicrobial resistance, it is important to determine the mechanism of action of AGPs and, subsequently, search
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Scalp bacterial species influence SIRT1 and TERT expression in keratinocytes. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-25 Azusa Yamada,Kota Watanabe,Yuri Nishi,Mugihito Oshiro,Yoshinori Katakura,Kenji Sakai,Yukihiro Tashiro
Scalp bacteria on the human scalp and scalp hair comprise distinct community structures for sites and individuals. To evaluate their effect on human keratinocyte cellular activity, including that of the hair follicular keratinocytes, the expression of several longevity genes was examined using HaCaT cells. A screening system that uses enhanced green fluorescent protein (EGFP) fluorescence was established
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Antidepressant properties of voluntary exercise mediated by gut microbiota. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-25 Chihiro Watanabe,Eri Oyanagi,Takafumi Aoki,Hiroki Hamada,Masato Kawashima,Takashi Yamagata,Michel J Kremenik,Hiromi Yano
Although regular exercise has been reported to prevent depression, it has not been clarified whether the gut microbiota is involved in the factors that prevent depression through exercise. We investigated the effects of voluntary exercise on the gut microbiota and the prevention of depression-like behaviors using mice. C57BL/6 J male mice were subjected to 10 weeks of sedentary control or wheel running
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Pentapeptide IIAEK ameliorates cholesterol metabolism via the suppression of intestinal cholesterol absorption in mice. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-25 Asahi Takeuchi,Yuyang Ye,Keigo Takada,Ryosuke Mori,Toma Nakamura,Natsuki Oda,Maihemuti Mijiti,Arata Banno,Satoshi Nagaoka
Dietary protein-derived peptides are effective in improving dyslipidemia and hypercholesterolemia. We previously identified a novel cholesterol-lowering pentapeptide IIAEK from milk beta-lactoglobulin. However, it remains unclear whether IIAEK affects the micellar solubility of cholesterol and the bile acid-binding ability to lower cholesterol. Moreover, there is no direct evidence that IIAEK inhibits
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Establishment of transgenic epithelium-specific Cre-recombinase driving medaka (Oryzias latipes) by homology repair mediated knock-in. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-25 Yuko Watanabe,Eri Katsumura,Tatsuki Domon,Yuta Ishikawa,Rina Oguri,Minami Takashima,Qi Meng,Masato Kinoshita,Hisashi Hashimoto,Kiyotaka Hitomi
Deletion of gene expression in the target tissues and cells is an effective strategy for elucidating the physiological functions of the protein of interest. For tissue-specific and/or inducible gene deletion, the Cre-loxP system has been widely used in various model organisms including medaka (Oryzias latipes). The epithelium is the key tissue, locating at the outermost area and playing a role in barrier
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3,6-Epidioxy-1,10-bisaboladiene and sulfasalazine synergistically induce ferroptosis-like cell death in human breast cancer cell lines. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-25 Narandulam Usukhbayar,Shota Uesugi,Ken-Ichi Kimura
3,6-Epidioxy-1,10-bisaboladiene (EDBD) is an endoperoxide compound isolated from edible wild plants that induces iron-dependent ferroptosis-like cell death in HL-60 cells by decreasing the expression of GPX4 and glutathione. In contrast, sulfasalazine (SSZ), a clinically used anti-inflammatory drug, induces ferroptosis through the system xc-. In this study, we investigated the synergistic effects of
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Erythritol aggravates gut inflammation and anxiety-like behavioral disorders induced by acute dextran sulfate sodium administration in mice. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-25 Yuzhi Jiang,Hailing Yin,Hongyu Wang,Ting Tao,Yong Zhang
Erythritol is a widely used sugar substitute in food and beverages with beneficial and detrimental roles in obesity and cardiovascular diseases, respectively; however, its influence on inflammatory bowel disease (IBD) and related behavioral disorders is not well understood. Here, we found that erythritol exacerbated gut inflammation by promoting macrophage infiltration and inducing M1 macrophage polarization
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Activity difference of three labdane diterpenoids on human constitutive androstane receptor. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-25 Mitsuhiro Sekiguchi,Yui Fujinami,Keiyu Takado,Yuu Kimoto,Yasuki Higashimura
The constitutive androstane receptor (CAR) regulates enzyme transcription related to drug metabolism; therefore, natural compound clarification in food that interacts with CAR is significant for drug development. We revealed that 13-epimanool, which is a compound found in the common sage, is bound to hCAR based on differential scanning fluorometry (DSF) measurements using recombinant hCAR protein.
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Identification and mechanistic investigation of ellagitannins from Osbeckia octandra that attenuate liver fibrosis via the TGF-β/SMAD signaling pathway. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-25 Sudarma Bogahawaththa,Tomoaki Kawamura,Udari Bandaranayake,Tomoaki Hirakawa,Goki Yamada,Hana Ishino,Tsuzumi Hirohashi,Shin-Ichi Kawaguchi,Kavindra K Wijesundera,Missaka P B Wijayagunawardane,Kanji Ishimaru,Suranga P Kodithuwakku,Tadayuki Tsujita
Fibrosis is a major problem in chronic liver disease with limited treatment options due to its complex nature. Herbal medicines are often used as an alternative. The aim of this study was to investigate the therapeutic potential of Osbeckia octandra and to identify its active compounds and regulatory pathways. The effects of crude leaf suspension and boiled leaf extract were investigated in an animal
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Secreted arabinogalactan protein from salt-adapted tobacco BY-2 cells appears to be glycosylphosphatidyl inositol-anchored and associated with lipophilic moieties. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-25 Arinze Boniface Nweke,Daiki Nagasato,Ken Matsuoka
Arabinogalactan proteins (AGPs) are plant extracellular proteoglycans associated with the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. This moiety is thought to be cleaved by phospholipase for secretion. Salt-adapted tobacco BY-2 cells were reported to secrete large amounts of AGPs into the medium. To investigate this mechanism, we expressed a fusion protein of tobacco sweet potato
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Optimization of multi-enzyme cascade process for the biosynthesis of benzylamine. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-25 Jinli Wang,Runan Dong,Jingxin Yin,Jianhua Liang,Haijun Gao
Benzylamine is a valuable intermediate in the synthesis of organic compounds such as curing agents and antifungal drugs. To improve the efficiency of benzylamine biosynthesis, we identified the enzymes involved in the multi-enzyme cascade, regulated the expression strength by using RBS engineering in Escherichia coli, and established a regeneration-recycling system for alanine. This is a cosubstrate
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BMP9 functions as a negative regulator in the myogenic differentiation of primary mouse myoblasts. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-25 Xiya Lu,Liang Li,Nanhui Wu,Wenjuan Chen,Sheng Hong,Mingyuan Xu,Yangfeng Ding,Yunlu Gao
BMP9, a member of the TGF-β superfamily, reveals the great translational promise for it has been shown to have the strong effect of osteogenic activity in vitro and in vivo. However, the implantation of certain BMPs (bone morphogenetic proteins) into muscular tissues induces ectopic bone formation. BMPs induce osteoblastic differentiation in skeletal muscle, suggesting that myogenic stem cells, such
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The effect of exogenous dihydroxyacetone and methylglyoxal on growth, anthocyanin accumulation, and the glyoxalase system in Arabidopsis. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-25 Maoxiang Zhao,Toshiyuki Nakamura,Yoshimasa Nakamura,Shintaro Munemasa,Izumi C Mori,Yoshiyuki Murata
Dihydroxyacetone (DHA) occurs in wide-ranging organisms, including plants, and can undergo spontaneous conversion to methylglyoxal (MG). While the toxicity of MG to plants is well-known, the toxicity of DHA to plants remains to be elucidated. We investigated the effects of DHA and MG on Arabidopsis. Exogenous DHA at up to 10 mm did not affect the radicle emergence, the expansion of green cotyledons
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The demand for ascorbate recycling capacity rises as the ascorbate pool size increases in Arabidopsis plants. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-25 Akane Hamada,Takahiro Ishikawa,Takanori Maruta
Ascorbate recycling is required for high ascorbate accumulation. Hence, when the ascorbate pool size is small, does the demand for ascorbate recycling decrease? We herein investigate the impact of ascorbate recycling capacity on ascorbate pool size in an ascorbate-deficient background. Our findings demonstrate that a smaller ascorbate pool size lowers the need for ascorbate recycling capacity even
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Ultrahigh-throughput screening of Trichoderma reesei strains capable of carbon catabolite repression release and cellulase hyperproduction using a microfluidic droplet platform. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-25 Xuan Chinh Luu,Yosuke Shida,Yoshiyuki Suzuki,Daiki Kuwahara,Takeshi Fujimoto,Yuka Takahashi,Naomi Sato,Akihiro Nakamura,Wataru Ogasawara
Trichoderma reesei is the most well-known cellulase producer in the biorefinery industry. Its cellulase biosynthesis is repressed by glucose via carbon catabolite repression (CCR), making CCR-releasing strains with cellulase hyperproduction desirable. Here, we employed a microfluidic droplet platform to culture and screen T. reesei mutants capable of CCR release and cellulase overproduction from extensive
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Peptide epimerase-dehydratase complex responsible for biosynthesis of the linaridin class ribosomal peptides. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-25 Wanlu Xiao,Takeshi Tsunoda,Chitose Maruyama,Yoshimitsu Hamano,Yasushi Ogasawara,Tohru Dairi
Grisemycin, salinipeptin, and cypemycin belong to the linaridin class of ribosomally synthesized and posttranslationally modified peptides that contain multiple dehydrobutyrine and D-amino acid residues. The biosynthetic gene clusters of these linaridins lack obvious candidate genes for the dehydratase and epimerase required to introduce dehydrobutyrine and D-amino acid residues, respectively. However
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Enhanced overexpression of secreted enzymes by discrete repeat promoters in Streptomyces lividans. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-25 Lingli Yang,Tadashi Hatanaka
Streptomyces lividans is an efficient host for extracellular overproduction of recombinant proteins. To enhance the overexpression strength of S. lividans, we designed several kinds of expression plasmids with different positioning of repeat promoters. The effect of repeat promoters was evaluated by measuring the accumulated amounts of a stable transglutaminase or an unstable carboxypeptidase that
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Betaine ameliorates heat stress-induced apoptosis by affecting oxidative and endoplasmic reticulum stress in mouse Leydig cells. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-20 Yongjie Xiong,Bing Li,Kang Wang,Jing Li,Shaojun He
In order to explore the potential protective role of betaine in heat stress (HS)-elicited apoptosis in mouse Leydig cells (mLCs). Betaine at 16 mM exerted a greater inhibitory effect on HS-induced viability attenuation of cells, which also significantly suppressed the heat shock protein 70 level in HS-treated cells. Furthermore, betaine ameliorated certain negative effects, including increased cell
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Effect of daily ingestion of Bifidobacterium and dietary fiber on vascular endothelial function: a randomized, double-blind, placebo-controlled, parallel-group comparison study. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-17 Naoki Azuma,Yasuo Saito,Tomohiko Nishijima,Ryo Aoki,Jun Nishihira
Bifidobacterium animalis subsp. lactis GCL2505 (GCL2505) improves the intestinal microbiota and reduces human visceral fat. This randomized, double-blind, placebo-controlled, parallel-group study was conducted to examine the effects of inulin, a prebiotic dietary fiber, and GCL2505 on vascular endothelial function in healthy subjects (n = 60). The test drink contained 2.0 g/100 g inulin and 1.0 × 1010
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Capsaicin indirectly regulates TRPA1 via the arachidonic acid cascade, resulting in TJ opening. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-14 Yusuke Kanda,Minagi Mukaiyama,Yohei Yamasaki,Takeo Usui,Yoko Nagumo
Capsaicin induces the reversible opening of tight junctions (TJs) and enhances the delivery of hydrophilic macromolecules through a paracellular route. We previously revealed that TRPA1 is involved in the capsaicin-induced Ca2+ influx and TJ permeability increase, although there are no reports that capsaicin directly activates TRPA1. In this study, we investigated the upstream factors of TRPA1 using
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Characterization of diterpene synthase genes in Brachypodium distachyon, a monocotyledonous model plant, provides evolutionary insight into their multiple homologs in cereals. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-13 Takeru Shimada,Shiho Minato,Yuto Hasegawa,Koji Miyamoto,Yasumasa Minato,Matthew R Shenton,Kazunori Okada,Hiroshi Kawaide,Tomonobu Toyomasu
Gibberellins are diterpenoid phytohormones that regulate plant growth, and are biosynthesized from a diterpene intermediate, ent-kaurene, which is produced from geranylgeranyl diphosphate via ent-copalyl diphosphate (ent-CDP). The successive two cyclization reactions are catalyzed by two distinct diterpene synthases, ent-CDP synthase (ent-CPS) and ent-kaurene synthase (KS). Various diterpene synthase
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Relationship between microorganisms and volatile components in each fermentation process in the kusaya gravy that plays an important role in the manufacturing of kusaya, a traditional Japanese fermented fish product. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-10 Kosuke Kita,Ryosuke Unno,Takahiro Osada,Hiromori Yoshiyama,Sachiko Masaki,Sakura Nogimura,Minenosuke Matsutani,Morio Ishikawa,Toshihiro Suzuki
The relationship between the microbiota and volatile components of kusaya gravy involved in the manufacturing of kusaya, a traditional Japanese fermented fish product, in the Izu Islands (Niijima and Hachijojima) and the fermentation processes are not clear. In this study, we aimed to investigate the relationship between the microbiota and volatile compounds involved in the manufacturing and management
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Differences in iron balance observed with dietary cholic acid supplementation and marginal iron deficiency in rats. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-09 Natsuki Kubota,Shota Hori,Satoshi Ishizuka
We investigated whether a cholic acid (CA)-supplemented diet and marginal iron deficiency (MID) diet influence hepatic lipid accumulation and iron balance in rats for two weeks. The CA diet enhanced hepatic lipid accumulation and modulated iron metabolism such as enhancement of fecal iron excretion, reduction in iron absorption, and no alteration in plasma iron levels. The MID diet did not alter hepatic
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Degradation of DDT by γ-HCH dehydrochlorinase LinA. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-05 Kafayat Olaide Yusuf Habibullah,Ren Ito,Leonardo Stari,Kouhei Kishida,Yoshiyuki Ohtsubo,Eiji Masai,Masao Fukuda,Keisuke Miyauchi,Yuji Nagata
1,1,1-Trichloro-2,2-bis(4-chlorophenyl)-ethane (DDT) is the first synthetic insecticide and one of the most widely used pesticides. The use of DDT has been banned, but it remains one of the most notorious environmental pollutants around the world. In this study, we found that γ-hexachlorocyclohexane (γ-HCH) dehydrochlorinase LinA from a γ-HCH-degrading bacterium, Sphingobium japonicum UT26, converts
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Direct screening for effective shRNA with a single mismatch in human cells without laborious cloning. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-04 Mayu Tachibana,Nazumi Ehara,Shunya Tanikawa,Akira Tachibana
We have devised a method for the direct screening of efficient short hairpin (sh)RNA molecules in human cells, eliminating the need for the time-consuming process of cloning in Escherichia coli. Our screening suggested that single mismatches to shRNAs can significantly alter their activity.
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Chemical screening of inhibitors specific for MdDOX-Co that cause an apple columnar tree-shape. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-10-03 Keisuke Okamoto,Taiki Inoue,Tsunesato Nagano,Sho Miyazaki,Ikuo Takahashi,Tadao Asami,Kazunori Okada,Kazuma Okada,Masatoshi Nakajima
MdDOX-Co, the ectopic expression of which is considered to cause the columnar tree shape, belongs to the 2-oxoglutarate-dependent dioxygenase (2ODD) family. It adds a hydroxyl group to position 12 of gibberellins. However, the 2ODD enzymes related to gibberellin biosynthesis and catabolism are phylogenetically distinct from MdDOX-Co. Thus, it is possible that substrates other than gibberellins exist
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The effect of Dunaliella tertiolecta supplementation on diet-induced obesity in UCP1-deficient mice. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-09-30 Yukari Yamashita,Tamaki Takeuchi,Yuki Endo,Ayumi Goto,Misa Uno,Setsuko Sakaki,Yuji Yamaguchi,Hiroyuki Takenaka,Hitoshi Yamashita
We previously demonstrated that dietary supplementation with Dunaliella tertiolecta (DT) increases uncoupling protein 1 (UCP1) expression in brown adipose tissue (BAT) and improves diet-induced obesity (DIO) in C57BL/6J mice at thermoneutrality (30°C). Here, we investigated whether DT improves DIO in a thermoneutral UCP1-deficient (KO) animal. KO mice were fed a high-fat diet supplemented with DT for
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Purification and characterization of 3-(5-oxo-2-thioxoimidazolidin-4-yl) propionic acid desulfhydrase involved in ergothioneine utilization in Burkholderia sp. HME13. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-09-27 Hisashi Muramatsu,Daisuke Inouchi,Masaaki Yamada,Akihito Koujitani,Hiroki Maguchi,Shin-Ichiro Kato
Recombinant 3-(5-oxo-2-thioxoimidazolidin-4-yl) propionic acid desulfhydrase (ErtC) derived from Burkholderia sp. HME13 was purified to homogeneity. Here, ErtC's kinetic parameters, optimum reaction temperature and pH, and stability at varying temperatures and pH and the effects of various additives on ErtC activity were determined. Real-time polymerase chain reaction and enzyme assays suggested that
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Luteolin inhibits Porphyromonas gingivalis growth and alleviates alveolar bone destruction in experimental murine periodontitis. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-09-22 Toru Kariu,Nobushiro Hamada,Kesavalu Lakshmyya
Periodontal disease is a major oral infectious disease that destroys alveolar bones and causes tooth loss. Porphyromonas gingivalis is a key pathogen that plays a crucial role in periodontitis. In our previous study on the anti-P. gingivalis activity of flavonoid, luteolin, a major flavonoid in edible plants, inhibited the proteolytic activity of gingipains, the major virulence factor in P. gingivalis
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Accumulation of squalene in filamentous fungi Trichoderma virens PS1-7 in the presence of butenafine hydrochloride, squalene epoxidase inhibitor: biosynthesis of 13C-enriched squalene. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-09-21 Wen Zhang,Kazu Sunami,Shuo Liu,Zihan Zhuang,Yasuko Sakihama,Da-Yang Zhou,Takeyuki Suzuki,Yuta Murai,Makoto Hashimoto,Yasuyuki Hashidoko
Squalene is a triterpenoid compound and widely used in various industries such as medicine and cosmetics due to its strong antioxidant and anticancer properties. The purpose of this study is to increase the accumulation of squalene in filamentous fungi using exogeneous butenafine hydrochloride, which is an inhibitor for squalene epoxidase. The detailed settings achieved that the filamentous fungi,
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CRISPR/Cas9 genome editing for comparative genetic analysis related to soy sauce brewing in Aspergillus sojae industrial strains. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-09-21 Takayuki Igarashi,Takuya Katayama,Jun-Ichi Maruyama
Aspergillus sojae has traditionally been used in soy sauce brewing. Genetic modification techniques have been established in A. sojae, but it is difficult to apply them to various industrial strains. Although we have previously developed a CRISPR/Cpf1 system for genetic modification of A. sojae, another genome editing system was required for versatile modification. In addition, repetitive genetic modification
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Hydrolysis-transglycosylation of sucrose and production of β-(2→1)-fructan by inulosucrase from Neobacillus drentensis 57N. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-09-21 Yusuke Kido,Wataru Saburi,Taizo Nagura,Haruhide Mori
Inulin, β-(2→1)-fructan, is a beneficial polysaccharide used as a functional food ingredient. Microbial inulosucrases (ISs), catalyzing β-(2→1)-transfructosylation, produce β-(2→1)-fructan from sucrose. In this study, we identified a new IS (NdIS) from the soil isolate, Neobacillus drentensis 57N. Sequence analysis revealed that, like other Bacillaceae ISs, NdIS consists of a glycoside hydrolase family
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Discovery of new functions of food proteins and their structural development for multifunctional applications. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-09-21 Akihito Ochiai
Proteins and peptides derived from various food sources are used in a variety of applications, including functional foods, pharmaceuticals, and cosmetics. The three-dimensional structure of proteins provides useful insights into their functions and essential information for the creation of proteins with new functions. In this review, a series of functional conversion technologies based on protein structural
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Improved electrochemical properties of graphite electrodes incubated with iron powders in rice-paddy fields boost power outputs from microbial fuel cells. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-09-21 Tomoka Harada,Yuki Hasegawa,Shinji Jomori,Masachika Inohana,Yuki Uno,Atsushi Kouzuma,Kazuya Watanabe
Studies have shown that the supplementation of anode-surrounding soil with zero-valent iron (ZVI) boosts power outputs from rice paddy-field microbial fuel cells (RP-MFCs). In order to understand mechanisms by which ZVI boosts outputs from RP-MFCs, the present study operated RP-MFCs with and without ZVI, and compositions of anode-associated bacteria and electrochemical properties of graphite anodes
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Enzymatic synthesis of β-d-fructofuranosyl α-d-glucopyranosyl-(1→2)-α-d-glucopyranoside using Escherichia coli glycoside phosphorylase YcjT. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-09-21 Naoto Isono,Shuichiro Yagura,Kanon Yamanaka,Yukino Masuda,Kazuki Mukai,Hirotaka Katsuzaki
YcjT is a kojibiose phosphorylase found in Escherichia coli. We found that sucrose was a good acceptor of YcjT in reverse phosphorolysis using β-d-glucose 1-phosphate as a donor. The product was identified as β-d-fructofuranosyl α-d-glucopyranosyl-(1→2)-α-d-glucopyranoside. This sugar was also synthesized from sucrose and maltose using YcjT and maltose phosphorylase and promoted the growth of the probiotic
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A novel endo-type chitinase possessing chitobiase activity derived from the chitinolytic bacterium, Chitiniphilus shinanonensis SAY3T. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-11-21 Sayaka Okazaki,Akane Komatsu,Moe Nakano,Goro Taguchi,Makoto Shimosaka
One of the chitinases (ChiG) derived from the chitinolytic bacterium Chitiniphilus shinanonensis SAY3T exhibited chitobiase activity cleaving dimers of N-acetyl-D-glucosamine (GlcNAc) into monomers, which is not detected in typical endo-type chitinases. Analysis of the reaction products for GlcNAc hexamers revealed that all the five internal glycosidic bonds were cleaved at the initial stage. The overall
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Modeling the structure of the transmembrane domain of T1R3, a subunit of the sweet taste receptor, with neohesperidin dihydrochalcone using molecular dynamics simulation. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-11-21 Tomoya Nakagita,Takumi Matsuya,Masataka Narukawa,Takuya Kobayashi,Takatsugu Hirokawa,Takumi Misaka
Neohesperidin dihydrochalcone (NHDC) is a sweetener, which interacts with the transmembrane domain (TMD) of the T1R3 subunit of the human sweet taste receptor. Although NHDC and a sweet taste inhibitor lactisole share similar structural motifs, they have opposite effects on the receptor. This study involved the creation of an NHDC-docked model of T1R3 TMD through mutational analyses followed by in
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Resveratrol inhibits basic fibroblast growth factor-induced macrophage colony-stimulating factor synthesis via the PI3-kinase/Akt pathway in osteoblasts. Biosci. Biotechol. Biochem. (IF 1.6) Pub Date : 2023-11-21 Gen Kuroyanagi,Tomoyuki Hioki,Junko Tachi,Rie Matsushima-Nishiwaki,Hiroki Iida,Haruhiko Tokuda,Osamu Kozawa
Resveratrol is a natural polyphenol found in grapes and beneficial for human health. Resveratrol regulates basic fibroblast growth factor (bFGF)-induced osteoprotegerin synthesis through Akt pathway in osteoblast-like MC3T3-E1 cells. In this study, we investigated resveratrol effects on bFGF-induced macrophage colony-stimulating factor (M-CSF) synthesis in MC3T3-E1 cells. bFGF significantly stimulated