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Application of targeted next generation sequencing for the mutational profiling of patients with acute lymphoblastic leukemia. J. Med. Biochem. Pub Date : 2020-01-10 Dragana Janic,Jelena Peric,Teodora Karan-Djurasevic,Tatjana Kostic,Irena Marjanovic,Bojana Stanic,Nadja Pejanovic,Lidija Dokmanovic,Jelena Lazic,Nada Krstovski,Marijana Virijevic,Dragica Tomin,Ana Vidovic,Nada Suvajdzic-Vukovic,Sonja Pavlovic,Natasa Tosic
Background Acute lymphoblastic leukemia (ALL) is the most common cancer in children, whereas it is less common in adults. Identification of cytogenetic aberrations and a small number of molecular abnormalities are still the most important risk and therapy stratification methods in clinical practice today. Next generation sequencing (NGS) technology provides a large amount of data contributing to elucidation
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Assessment of Abbott Architect 25-OH vitamin D assay in different levels of vitamin D. J. Med. Biochem. Pub Date : 2020-01-10 Esin Avci,Süleyman Demir,Diler Aslan,Rukiye Nar,Hande Şenol
Background There is increasing requests of Vitamin D test in many clinical settings in recent years. However, immunoassay performance is still a controversial topic. Several diagnostic manufacturers have launched automated 25-hydroxyvitamin D (25-OH D) immunoassays in the past decade. We compared the performance of Abbott Architect 25-OH D Vitamin immunoassay with liquid chromatography-tandem mass
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NLRP3 and CARD8 polymorphisms influence risk for asbestos-related diseases. J. Med. Biochem. Pub Date : 2020-01-10 Alenka Franko,Katja Goricar,Viljem Kovac,Metoda Dodic-Fikfak,Vita Dolzan
Background This study aimed to investigate the association between NLRP3 rs35829419 and CARD8 rs2043211 polymorphisms and the risk of developing pleural plaques, asbestosis, and malignant mesothelioma (MM), and to study the influence of the interactions between polymorphisms and asbestos exposure on the risk of developing these diseases. Methods The case-control study included 416 subjects with pleural
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Urinary nephrin is earlier, more sensitive and specific marker of diabetic nephropathy than microalbuminuria. J. Med. Biochem. Pub Date : 2020-01-10 Irena Kostovska,Katerina Tosheska-Trajkovska,Sonja Topuzovska,Svetlana Cekovska,Goce Spasovski,Ognen Kostovski,Danica Labudovic
Background Diabetic nephropathy (DN) is a leading cause of end-stage renal disease. Progressive damage and decline in the number of podocytes often occur in the early stages of DN. Thus, nephrin as a podocyte-specific protein may be regarded as a potential biomarker of early detection of DN. The aim of this study is to determine whether urinary nephrin is an earlier marker in DN than microalbuminuria
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Leukocytosis interference in clinical chemistry: Shall we still interpret test results without hematological data? J. Med. Biochem. Pub Date : 2020-01-10 Guillaume Grzych,Estelle Roland,David Beauvais,Patrice Maboudou,Giuseppe Lippi
Background Extreme leukocytosis is known to induce remarkable variations of some clinical chemistry tests, thus leading to possible clinical misinterpretation. This study aimed to define whether also moderate leukocytosis may influence the stability of glucose and blood gases. Methods Blood samples are sent to the local laboratory through a pneumatic tube system. Clinical chemistry testing is routinely
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Peripheral neural response and sex hormones in type 1 Gaucher disease. J. Med. Biochem. Pub Date : 2020-01-10 Milan B Lakočević,Mirjana M Platiša,Zorica R Šumarac,Nada D Suvajdžić,Lana Đ Mačukanović,Milan S Petakov
Background In a rare Gaucher disease, reduced activity of lysosomal b-glucocerebrosidase incompletely blocks glucosphingolipid catabolism. Accumulation of the unhydrolyzed substrate glucosylceramide within lysosomes results in progressive, multisystem Gaucher disease, classified into three types. Both parkinsonism and peripheral neuropathy are observed in cases of putative non-neuronopathic type 1
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Platelet glutamate dehydrogenase activity and efficacy of antipsychotic therapy in patients with schizophrenia. J. Med. Biochem. Pub Date : 2020-01-10 Olga K Savushkina,Elena B Tereshkina,Tatiana A Prokhorova,Irina S Boksha,Denis S Burminskii,Elena A Vorobyeva,Margarita A Morozova,Gulnur Sh Burbaeva
Background Evaluation of possible relationship between platelet glutamate dehydrogenase (GDH) activity and mental state of schizophrenia patients after antipsychotic pharmacotherapy. Methods Patients (n = 50) with chronic paranoid schizophrenia (F20.0) initially in acute psychotic state were examined before and after a treatment course with antipsychotics. When assessing the patients' states using
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Changes in the blood fatty-acid profile associated with oxidative-antioxidant disturbances in coronary atherosclerosis. J. Med. Biochem. Pub Date : 2020-01-10 Yuliya I Ragino,Viktoriya S Shramko,Ekaterina M Stakhneva,Elena I Chernyak,Sergey V Morozov,Elena V Shakhtshneider,Yana V Polonskaya,Liliia V Shcherbakova,Alexander M Chernyavskiy
Background The objective of this work was to study the profile of fatty acids and to search for associations of fatty acids with oxidative-antioxidant parameters and an oxidative-inflammatory biomarker (lipoprotein-associated phospholipase A2) in men with coronary atherosclerosis and coronary heart disease. Methods Analysis of 20 fatty acids was performed in 60 men with angiographically confirmed coronary
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Effects of an eight-week exercise program on parameters of the lipid profile of female students. J. Med. Biochem. Pub Date : 2020-01-10 Irina Juhas,Branko Skof,Dejana Popović,Milan Matić,Nenad Janković
Background Increased lipid levels are one of the major risk factors for the development of cardiovascular diseases. The aim of the current study was to evaluate the effect of short-term (8 weeks) aerobic exercise of moderate to vigorous intensity on lipid profile in young healthy females. Methods 27 female students (mean age 20.5 ± 1 year) completed 8 weeks of aerobic training that included two exercise
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Glucose meters: What's the laboratory reference glucose? J. Med. Biochem. Pub Date : 2020-01-10 Mariarosa Carta,Davide Giavarina,Andreina Paternoster,Graziella Bonetti
Background The accuracy of glucose meters is evaluated by comparing their results with those from a reference laboratory glucose analyser. The main scientific societies recommend the use of a prompt glycolysis inhibitor such as citrate for an accurate glucose determination. In the present preliminary study, we discuss the bias between capillary and plasma glucose measured concentrations, determined
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The relationship between sex steroids, insulin resistance and body compositions in obese women: A case-control study. J. Med. Biochem. Pub Date : 2020-01-10 Seher Sayın,Ruhuşen Kutlu,Mustafa Kulaksızoğlu
Background Obesity causes many health problems and affects the quality and duration of life negatively. We aimed to investigate the relationship between sex steroids, insulin resistance and body compositions in obese women. Methods This study was carried out on a sample of 150 premenopausal women who were referred to the Outpatient Clinic of Family Medicine between 2014-2015. A survey about their socio-demographic
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Thrombin generation in different commercial sodium citrate blood tubes. J. Med. Biochem. Pub Date : 2020-01-10 Salvagno Gian Luca,Davide Demonte,Matteo Gelati,Giovanni Poli,Emmanuel J Favaloro,Giuseppe Lippi
Background This study aimed to verify whether blood drawn into six different commercial coagulation tubes generated comparable results of thrombin generation. Methods Blood was sequentially collected from 20 healthy subjects into different brand and draw volume 3.2% sodium citrate tubes (4.3 mL Sarstedt, 3.0 mL Greiner, 2.7 mL Becton Dickinson, 2.0 mL Kima, 1.8 mL Sarstedt and 1.0 mL Greiner). Thrombin
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Measuring plasma ferritin levels with two different methods: A comparison of Roche Cobas e601 versus Roche Cobas c501 (integrated modular system Roche Cobas 6000). J. Med. Biochem. Pub Date : 2020-01-10 Durmuş Ayan,Sibel Soylemez
Background The aim of our study is to compare plasma ferritin levels found to be high or low in terms of reference range by means of electrochemiluminescence (ECLIA) and immunoturbidimetric method and to examine whether they can be used interchangeably. Methods 84 patients with high plasma ferritin level and 153 patients with low ferritin level according to the re ference range were included in the
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Can neutrophil/lymphocyte ratio be used as an indicator of inflammation in patients with hyperthyroidism? J. Med. Biochem. Pub Date : 2020-01-10 Murat Dağdeviren,Tolga Akkan,Dilek Yapar,Serdar Karakaya,Tanyel Dağdeviren,Derun Ertuğrul,Mustafa Altay
Background In our study, we aimed to evaluate changes in the neutrophil and lymphocyte series and investigate whether the neutrophil/lymphocyte ratio (NLR) is indicative of inflammations in patients with hyperthyroidism. Methods A total of 161 patients were enrolled, 121 of which had hyperthyroidism (71 Graves' Disease (GD) and 50 non-Graves hyperthyroidism (NGH) patients) and 40 of which were control
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Vitamin D and thiol-disulfide homeostasis levels in postmenopausal women with overactive bladder syndrome. J. Med. Biochem. Pub Date : 2020-01-10 Yasemin Ustundag,Karlıbel Lknur Aykurt,Murat Sambel,Murat Ozturk,Atilla Satır,Elif Yolgosteren,Salim Neselioglu,Ozcan Erel
Background This study aimed to find a relationship between vitamin D concentration and thiol-disulfide homeostasis in the pathophysiology of overactive bladder (OAB) syndrome in postmenopausal women. Methods A total of 76 postmenopausal women, referred for routine controls, were recruited between January and March 2018 to participate in this study. Participants with an overactive bladder questionnaire
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Diagnostic benefits of mindin as a prostate cancer biomarker: Dijagnostičke prednosti mindina kao biomarkera raka prostate. J. Med. Biochem. Pub Date : 2020-01-10 Lenka Hanousková,Jakub Řezáč,Štěpán Veselý,Richard Průša,Karel Kotaška
Background It has been shown that decreased expression and activity of extracellular matrix protein mindin correlate with various types of cancers including breast, colon and lung cancers. The aim of the presented study was to investigate the serum mindin levels in prostate cancer. Methods Mindin concentrations in serum were measured in 56 patients with prostate cancer (mean age 68 years) and in control
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Thiol-disulphide Homeostasis in Essential Thrombocythemia Patients. J. Med. Biochem. Pub Date : 2019-07-30 Aysun Şentürk Yıkılmaz,Şule Mine Bakanay,Sema Akinci,Murat Alisik,Özcan Erel,İmdat Dilek
Background This study aimed to show the status of thioldisulphide homeostasis in essential thrombocytosis patients, which is known to play a role in platelet function. Methods The study included 27 ET patients and a control group of 36 healthy subjects. Serum total (-SH + -S-S-) and native (-SH) thiol levels were measured in all subjects using an automatic method. Results Age and gender distribution
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Red Blood Cell Distribution Width Predicts 1-month Complications after Percutaneous Transluminal Angioplasty. J. Med. Biochem. Pub Date : 2019-07-30 Gian Franco Veraldi,Luca Mezzetto,Lorenzo Scorsone,Marco Macrì,Fabio Simoncini,Giuseppe Lippi
Background The identification of patients at higher risk of developing percutaneous transluminal angioplasty (PTA)-related complications is pivotal for achieving better clinical outcomes. We carried out a single-center, observational, retrospective study to explore whether in-hospital changes of red blood cell distribution width (RDW) may help predicting early development of PTA-related complications
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Evaluation of Neutrophil-lymphocyte and Platelet-lymphocyte Ratios as Predictors of 30-day Mortality in Patients Hospitalized for an Episode of Acute Decompensated Heart Failure. J. Med. Biochem. Pub Date : 2019-07-30 Gianni Turcato,Fabian Sanchis-Gomar,Gianfranco Cervellin,Elisabetta Zorzi,Valentina Sivero,Gian Luca Salvagno,Andrea Tenci,Giuseppe Lippi
Background To investigate the association between both neutrophil to lymphocyte ratio (NLR) and platelet to lymphocyte ratio (PLR) and 30-day mortality in patients hospitalized for an episode of acute decompensated heart failure (ADHF). Methods 439 patients admitted to emergency department (ED) for an episode of ADHF. Clinical history, demographic, clinical and laboratory data recorded at ED admission
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Correlation of Fgf23 and Balp with Bone Mineral Density in Hemodialysis Patients. J. Med. Biochem. Pub Date : 2019-07-30 Mouna Bouksila,Mehdi Mrad,Wajih Kaabachi,Eya Kalai,Wided Smaoui,Sonia Rekik,Asma Krir,Nesrine Issaoui,Kamel Hamzaoui,Hela Sahli,Elhem Cheour El Kateb,Mohammed Karim Zouaghi,Afef Bahlous
Background Chronic kidney disease (CKD) is associated with numerous complications such as bone mineral disorder. The aim of our study was to analyze the correlation of bone turnover markers with Bone Mineral Density (BMD) measurements in Tunisian end stage renal diseases (ESRD) patients. Methods This study included 100 ESRD Tunisian patients. Their estimated glomerular filtration rate (eGFR) was <
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Project Management in Laboratory Medicine. J. Med. Biochem. Pub Date : 2019-07-30 Giuseppe Lippi,Camilla Mattiuzzi
The role and responsibilities of laboratory managers have considerably evolved during the past decades. This revolution has been mostly driven by biological, technical, economic and social factors, such as deepened understanding of the pathophysiology of human diseases, technical innovations, renewed focus on patient safety, cost-containment strategies and patient empowerment. One of the leading consequences
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Association of Serum Paraoxonase 1 Activities, Polymorphisms and Oxidative Stress in Breast Cancer Patients with Type 2 Diabetes Mellitus. J. Med. Biochem. Pub Date : 2019-05-11 Fatma Ceyla Eraldemir,Nihal Üren,Tuğba Kum,Burcu Erbay,Deniz Şahin,Emel Ergül,Esra Acar,Doğa Özsoy,Mustafa Çekmen,Hale Kır,Zafer Utkan
Background The aim of the study was to investigate the association of paraoxonase 1 (PON1) polymorphism, PON1/arylesterase (ARE) activity and oxidative stress index (OSI) in breast cancer (BC) patients with type 2 diabetes (DM). Methods Our study group consisted of 30 healthy women (HV group) and 66 female BC patients. The BC patients were divided into two groups: those with (n=37) and without DM (n=29)
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Nitric Oxide Products are not Associated with Metabolic Syndrome. J. Med. Biochem. Pub Date : 2019-05-11 Aleksandra Klisic,Gordana Kocic,Nebojsa Kavaric,Radmila Pavlovic,Ivan Soldatovic,Ana Ninic
Background Nitric oxide (NO) is oxidative stress biomarker which is regarded as one of the key determinants of energy metabolism and vascular tone. Considering the controversial reports on the association between nitric oxide products (NOx) and metabolic syndrome (MetS), the aim of the current study was to examine that potential relationship. Additionally, we aimed to evaluate a broad spectrum of other
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Serum C-C Motif Ligand 11/eotaxin-1 May Serve as a Candidate Biomarker for Postmenopausal Osteoporosis. J. Med. Biochem. Pub Date : 2019-05-11 Wen Wang,Ci-You Huang,Zhuo-Ping Wang,Shan-Shan Xu,Tie-Yong Qian,Yi-Ding Chen,Wei-Guo Wu
Background The chemokine C-C motif ligand 11, also known as eotaxin-1, has been identified as a novel mediator of inflammatory bone resorption. However, little is known regarding a potential role for CCL11/Eotaxin-1 in postmenopausal osteoporosis. Objective The scope of this study was to explore the relationship between serum CCL11/Eotaxin-1 concentrations and disease progression of postmenopausal
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T Cell Subsets' Distribution in Elite Karate Athletes as a Response to Physical Effort. J. Med. Biochem. Pub Date : 2019-05-11 Dorota Kostrzewa-Nowak,Rafał Buryta,Robert Nowak
Background During karate fight muscles work at a very high intensity, and their contractions are extremely strong. The movement pattern contains a great number of feints, dodges, frequent changes in movements' tempo and direction, hits and kicks, all of which is highly stressful for athlete's organism, including the immune system. Methods T lymphocyte subsets' distribution and selected cytokines in
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Soluble sPD-L1 and Serum Amyloid A1 as Potential Biomarkers for Lung Cancer. J. Med. Biochem. Pub Date : 2019-05-11 Dragana Jovanović,Marina Roksandić-Milenković,Jelena Kotur-Stevuljević,Vesna Ćeriman,Ivana Vukanić,Natalija Samardžić,Spasoje Popević,Branislav Ilić,Milija Gajić,Marioara Simon,Ioan Simon,Vesna Spasojević-Kalimanovska,Milica Belić,Damjan Mirkov,Zorica Šumarac,Vladislav Milenković
Background The objective of this prospective study was to evaluate whether soluble programmed cell death-1/programmed cell death-ligand 1 (PD-1/PD-L1) and serum amyloid A1 (SAA1) are potential diagnostic, predictive or prognostic biomarkers in lung cancer. Methods Lung cancer patients (n=115) with advanced metastatic disease, 101 with non-small cell lung cancer, NSCLC (77 EGFR wild-type NSCLC patients
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Exploring Sirolimus Pharmacokinetic Variability Using Data Available from the Routine Clinical Care of Renal Transplant Patients - Population Pharmacokinetic Approach. J. Med. Biochem. Pub Date : 2019-05-11 Bojana Golubović,Katarina Vučićević,Dragana Radivojević,Sandra Vezmar Kovačević,Milica Prostran,Branislava Miljković
Background Due to wide intra- and inter-individual pharmacokinetic variability and narrow therapeutic index of sirolimus, the therapeutic drug monitoring (TDM) of sirolimus with detailed biochemical and clinical monitoring is necessary for dose individualization in kidney transplant patients. The purpose of the study was to explore and identify factors that contribute to pharmacokinetic variability
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Determining the Need for Repeat Testing of Blood Ethanol Concentration: Evaluation of the Synchron Blood Ethyl Alcohol Assay Kit. J. Med. Biochem. Pub Date : 2019-05-11 Yasemin Ustundağ,Kağan Huysal,Sevim Eşmedere Eren
Background In clinical laboratories, a common practice used to verify tests prior to reporting is repeat testing. Our objective was to evaluate the differences between the results of blood ethanol concentration (BEC) test repetitions and report on the role of repeat testing to prevent reporting of incorrect results. Methods We conducted a retrospective study of data retrieved from the Bursa Yuksek
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Effects of Timolol Treatment on Pancreatic Antioxidant Enzymes in Streptozotocin-induced Diabetic Rats: An Experimental and Computational Study. J. Med. Biochem. Pub Date : 2019-05-11 Nuriye Nuray Ulusu,Muslum Gok,Burak Erman,Belma Turan
Background The study aimed to investigate whether timolol-treatment has a beneficial effect on pentose phosphate pathway enzyme activities such as glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGDH) enzyme activities and cAMP level in streptozotocin-induced diabetic rats in pancreatic tissues. Methods Diabetes was induced by streptozotocin (STZ) in 3-month old male Wistar
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A Novel Biomarker Renalase and Its Relationship with its Substrates in Schizophrenia. J. Med. Biochem. Pub Date : 2019-05-11 Zekiye Catak,Esra Kocdemir,Kader Ugur,Meltem Yardim,İbrahim Sahin,Hilal Kaya,Suleyman Aydin
Background Schizophrenia, particularly the form related to excessive dopamine (DA), is a chronic psychotic disorder affecting millions of people worldwide. Renalase metabolizes its catecholamine (CA) substrates, including DA, suggesting that there might be an association between renalase levels and schizophrenia occurrence. Therefore, the current study aimed to evaluate the renalase and CA levels in
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Expression Pattern of Long Non-coding RNA Growth Arrest-specific 5 in the Remission Induction Therapy in Childhood Acute Lymphoblastic Leukemia. J. Med. Biochem. Pub Date : 2019-05-11 Vladimir Gasic,Biljana Stankovic,Branka Zukic,Dragana Janic,Lidija Dokmanovic,Nada Krstovski,Jelena Lazic,Goran Milosevic,Marianna Lucafò,Gabriele Stocco,Giuliana Decorti,Sonja Pavlovic,Nikola Kotur
Background Long non-coding RNA growth arrest-specific 5 (GAS5) is deregulated in many cancers because of its role in cell growth arrest and apoptosis. Additionally, GAS5 interacts with glucocorticoid receptor, making it a potential pharmacotranscription marker of glucocorticoid (GC) therapy. In this study, we aimed at analysing GAS5 expression in the remission induction therapy phase of childhood acute
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Superoxide Dismutase Isoenzymes Gene Expression in Peripheral Blood Mononuclear Cells in Patients with Coronary Artery Disease. J. Med. Biochem. Pub Date : 2019-05-11 Ana Ninić,Nataša Bogavac-Stanojević,Miron Sopić,Jelena Munjas,Jelena Kotur-Stevuljević,Milica Miljković,Tamara Gojković,Dimitra Kalimanovska-Oštrić,Vesna Spasojević-Kalimanovska
Background Coronary artery disease (CAD) is one of the most important causes of mortality and morbidity in wide world population. Dyslipidemia, inflammation and oxidative stress may contribute to disruption of endothelium structure and function, atherosclerosis and CAD. Our study was aimed to determine whether Cu/Zn superoxide dismutase (Cu/Zn SOD) and Mn superoxide dismutase (Mn SOD) gene expression
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TNF-α and IL-6 as Biomarkers of Impaired Lung Functions in Dimethylacetamide Exposure. J. Med. Biochem. Pub Date : 2019-05-11 Lütfiye Tutkun,Servet Birgin İritaş,Serdar Deniz,Özgür Öztan,Sedat Abuşoğlu,Ali Ünlü,Vugar Ali Türksoy,Sultan Pınar Çetintepe
Background Tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) are well-known biomarkers of systemic inflammation that have been associated with many diseases in the past. In this study, we aimed to determine the relationship between impaired lung functions and the levels of these biomarkers in DMAc exposed people. Methods 101 non-exposed control subjects (Group 1) and 109 DMAc-exposed workers
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Plasma Cathepsin S is Associated with High-density Lipoprotein Cholesterol and Bilirubin in Patients with Abdominal Aortic Aneurysms. J. Med. Biochem. Pub Date : 2019-05-11 Bosa Mirjanic-Azaric,Novak Vasic,Darko Cerne,Janko Kos,Natasa Bogavac-Stanojevic
Background Cathepsin S (CTSS) is a cysteine protease involved in atherogenesis. We compared the plasma CTSS as well as other biomarkers of atherosclerosis in patients with abdominal aortic aneurysms (AAA) and aortoiliac occlusive disease (AOD), aiming to identify the underlying pathogenic mechanisms of the disease development. Also, we hypothesised that the level of plasma CTSS simultaneously increases
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Comparison of Selected CD45+ Cell Subsets' Response and Cytokine Levels on Exhaustive Effort Among Soccer Players. J. Med. Biochem. Pub Date : 2019-05-11 Dorota Kostrzewa-Nowak,Rafał Buryta,Robert Nowak
Background Immunological alterations may led to the reduction in capacity and endurance levels in elite athletes by e.g. increased susceptibility to infections. There is a need to explain the impact of intensive physical effort on the CD4+ memory T cell subsets. Methods Fourteen participants median aged 19 years old (range 17-21 years) were recruited form Pogoń Szczecin S.A., soccer club. They performed
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The Effect of Effort Test on the Levels of Ischemia Modified Albumin, 7-ketocholesterol and Cholestan-3β , 5α , 6β -triol and their Role in the Diagnosis of Coronary Artery Disease. J. Med. Biochem. Pub Date : 2019-05-11 Muhammed Emin Keles,Afshin Samadi,Selen Yilmaz Isikhan,Yusuf Ziya Sener,Ali Sezgin,Emine Keles,Incilay Lay,Uğur Canpolat
Background Oxysterols have been shown to play a role in plaque formation while ischemia modified albumin (IMA) is widely accepted as an acute marker for ischemia. The effort test is one of the methods used to identify the presence of coronary artery disease. Thus, there may be a relationship between effort test result and the levels of IMA, 7-ketocholesterol (7-KC) and cholestane-3β,5α,6β-triol (C-triol)
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Evaluation of Paraoxonase, Arylesterase, and Homocysteine Thiolactonase Activities in Patients with Diabetes and Incipient Diabetes Nephropathy. J. Med. Biochem. Pub Date : 2019-03-26 Durmuş Ayan,Mehmet Şeneş,Ayşe Banu Çaycı,Sibel Söylemez,Nezaket Eren,Yüksel Altuntaş,Feyza Yener Öztürk
Background The aim of this study is to examine the relationship among the changes in activities of paraoxonase (PON), arylesterase (ARE) and homocysteine thiolactonase (HTLase) enzyme having antioxidant properties and the development of diabetic nephropathy (DN), one of the most common complications of diabetes. Methods Normoalbuminuric type-2 diabetic patients (Group II, n=100), microalbuminuric type
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Thiol/Disulphide Homeostasis, Ischemia Modified Albumin, and Ferroxidase as Oxidative Stress Markers in Women with Obesity with Insulin Resistance. J. Med. Biochem. Pub Date : 2019-03-26 Elif Ates,Turan Set,Süleyman Caner Karahan,Cemile Biçer,Özcan Erel
Background The purpose of the study was to determine oxidative stress-related plasma thiol/disulphide, ischemia-modified albumin (IMA) levels and ferroxidase activity among women with obesity in insulin-resistant and non-insulin-resistant groups in comparison with an overweight group. Methods We compared plasma thiol/disulphide, IMA levels, and ferroxidase activity between the study groups. We analyzed
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Assessment of Selected Exercise-induced CD3+ Cell Subsets and Cell Death Parameters Among Soccer Players. J. Med. Biochem. Pub Date : 2019-03-26 Robert Nowak,Dorota Kostrzewa-Nowak
Background Molecular mechanisms of biological adaptation to training in professional soccer players are unclear. The aim of this study was to assess the impact of progressive physical effort on peripheral T-cells and their molecular response. Methods Thirteen soccer players form Pogo Szczecin S.A., a top league soccer club, (median age 21, range 18- 31, years old) performed progressive efficiency tests
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Body Mass Index and C-reactive Protein Are Potential Predictors of Asthma Development in Egyptian Polycystic Ovary Syndrome Patients. J. Med. Biochem. Pub Date : 2019-03-26 Hebatallah Ahmed Nasser,Nermeen Zakaria Abuel Ezz,Heidi Mohamed Abdel-Mageed,Rasha Ali Radwan
Background Recent studies suggest asthma prevalence in polycystic ovary syndrome (PCOS) patients. This is the first study to explore asthma prevalence among Egyptian PCOS patients. It highlighted common findings in PCOS and asthma. It investigated whether these findings could serve as potential predictors of asthma. Methods A hundred PCOS patients, sixty asthmatic patients and thirty apparently healthy
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Predictive Values of Serum Uric Acid and Alanine-aminotransferase for Fatty Liver Index in Montenegrin Population. J. Med. Biochem. Pub Date : 2019-03-26 Aleksandra Klisic,Nebojsa Kavaric,Ana Ninic
Background Alanine-aminotransferase (ALT) and uric acid cut-off levels used in non-alcoholic fatty liver disease (NAFLD) diagnosis are advised to be lowered. Due to contradictory results on the utility of both these biomarkers for NAFLD screening, we aimed to determine their cut-off levels that can be applied to Montenegrin population with the fatty liver disease. Methods A total of 771 volunteers
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Association of Overexpressed MYC Gene with Altered PHACTR3 and E2F4 Genes Contributes to Non-small Cell Lung Carcinoma Pathogenesis. J. Med. Biochem. Pub Date : 2019-03-03 Miodrag Dragoj,Jasna Bankovic,Ana Podolski-Renic,Sonja Stojkovic Buric,Milica Pesic,Nikola Tanic,Tijana Stankovic
Background C-Myc is one of the major cellular oncogenes overexpressed in non-small cell lung carcinoma (NSCLC). Its deregulated expression is necessary but not sufficient for malignant transformation. We evaluated expression of MYC gene in NSCLC patients and its association with alterations in the genes previously identified to be related to NSCLC pathogenesis, PHACTR3 and E2F4. Methods We analyzed
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Vitamin D Levels of Outpatients Admitted to a University Hospital in the Marmara Region of Turkey Over 3 Years. J. Med. Biochem. Pub Date : 2019-03-03 Gulbuz Sezgin,Guler Ozturk,Rana Turkal,Burcu Caykara
Background Vitamin D regulates calcium and phosphorus metabolism, and it is essential for bone formation. Several factors can affect vitamin D levels in plasma. In present study we compare vitamin D levels of outpatients, who admit to Maltepe University Hospital between 2011 and 2013 and had vitamin D measurements regarding gender, age, and season. Methods Hospital records were evaluated to identify
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Development of a Quantitative Detection Card for Heart-type Fatty Acid-binding Protein based on Background Fluorescence Quenching Immune Chromatography. J. Med. Biochem. Pub Date : 2019-03-03 Wei Zhang,Junlei Chen,Xinxia Li,Yuwen Wang,Jiutong Li
Background To establish a fast and simple quantitative method for detection of heart-type fatty acid-binding protein (H-FABP) in serum based on a background fluorescence quenching immunochromatographic assay. Methods A detection card based on the double-antibody sandwich double-antibody method with background fluorescence quenching was developed for quantitative measurement of H-FABP in serum. The
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Relationship Between Hematological Parameters and Glycemic Control in Type 2 Diabetes Mellitus Patients. J. Med. Biochem. Pub Date : 2019-03-03 Dragana Milosevic,Violeta Lukic Panin
Background Diabetes mellitus (DM) with its micro- and macrocomplications is the leading global epidemic of the 21st century. The aim of the research is to determine possible changes in the complete blood count (CBC) parameters depending on glycemic controlin patients with Type 2 diabetes mellitus (T2DM). Methods The study included a total of 178 patients with T2DM, both gender over the age of 40 years
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Association of FTO Gene Variant (rs8050136) with Type 2 Diabetes and Markers of Obesity, Glycaemic Control and Inflammation. J. Med. Biochem. Pub Date : 2019-03-03 Tamer Bego,Adlija Čaušević,Tanja Dujić,Maja Malenica,Zelija Velija-Asimi,Besim Prnjavorac,Janja Marc,Jana Nekvindová,Vladimír Palička,Sabina Semiz
Background FTO, a gene recently discovered in genomewide associated studies for type 2 diabetes mellitus (T2D), play an important role in the management of energy homeostasis, nucleic acid demethylation and regulation of body fat mass by lipolysis. The aim of this study was to analyze the association of FTO rs8050136 A>C genetic variant with clinical and biochemical parameters of T2D in the population
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A Pilot Study to Assess Kidney Functions and Toxic Dimethyl-arginines as Risk Biomarkers in Women with Low Vitamin D Levels. J. Med. Biochem. Pub Date : 2019-03-03 Samar Damiati
Background Although vitamin D in not a traditional marker for cardiovascular and renal diseases, several studies have proposed a correlation between vitamin D deficiency and these diseases due to the effect of vitamin D on endothelial function. Asymmetric and symmetric dimethyl arginine (ADMA and SDMA, respectively) are endogenous markers of endothelial dysfunction, and are considered as future markers
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Dual Roles of the Mineral Metabolism Disorders Biomarkers in Prevalent Hemodilysis Patients: In Renal Bone Disease and in Vascular Calcification. J. Med. Biochem. Pub Date : 2019-03-03 Marko Baralić,Voin Brković,Vesna Stojanov,Sanja Stanković,Nataša Lalić,Petar Đurić,Ljubica Đukanović,Milorad Kašiković,Milan Petrović,Marko Petrović,Milan Stošović,Višnja Ležaić
Background Vascular calcification (VC) is highly prevalent in dialysis (HD) patients, and its mechanism is multifactorial. Most likely that systemic or local inhibitory factor is overwhelmed by promoters of VC in these patients. VC increased arterial stiffness, and left ventricular hypertrophy. Thus, the present study aimed to investigate the association of VC and myocardial remodeling and to analyze
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The Inflammatory and Hemostatic Cardiovascular Risk Markers During Acute Hyperglycemic Crisis in Type 1 and Type 2 Diabetes. J. Med. Biochem. Pub Date : 2019-03-03 Dragana Popovic,Katarina Lalic,Aleksandra Jotic,Tanja Milicic,Jelena Bogdanovic,Maja Đorđevic,Sanja Stankovic,Veljko Jeremic,Nebojsa M Lalic
Background We analyzed cardiovascular inflammatory (C-reactive protein (CRP), interleukin 6 (IL-6)), haemostatic (homocysteine) risk markers in lean and obese patients at admission and acute hyperglicemic crisis (AHC) resolving, involving diabetic ketoacidosis (DKA) and hyperosmolar hyperglycemic state (HHS). Methods In that context, we included group A: N = 20 obese, B: N=20 lean patients with DKA;
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Serum Lactate As Reliable Biomarker of Acute Kidney Injury in Low-risk Cardiac Surgery Patients. J. Med. Biochem. Pub Date : 2019-03-03 Mina Radovic,Suzana Bojic,Jelena Kotur-Stevuljevic,Visnja Lezaic,Biljana Milicic,Milos Velinovic,Radmila Karan,Sanja Simic-Ogrizovic
Background Cardiac surgery-associated acute kidney injury (CSA-AKI) frequently occurs in patients assessed as low-risk for developing CSA-AKI. Neutrophil Gelatinase-Associated Lipocalin (NGAL), Kidney Injury Molecule-1 (KIM-1) and lactate are promising biomarkers of CSA-AKI but have not yet been explored in low-risk patients. Aim To evaluate urinary NGAL (uNGAL), KIM-1 and lactate as biomarkers of
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Circulating MiR-210 and MiR-1246 as Potential Biomarkers for Differentiating Hepatocellular Carcinoma from Metastatic Tumors in the Liver. J. Med. Biochem. Pub Date : 2019-03-03 Emad K Ahmed,Shaimaa A Fahmy,Heba Effat,Abdel Hady Abdel Wahab
Background To date few reports have pointed out the role of circulating miRNAs in discriminating metastatic liver tumors from primary hepatocellular (HCC) tumors. Such discrimination will have significant therapeutic and prognostic implications. The purpose of this study was to evaluate the potential value of a panel of HCC-related circulating miRNAs (miR-142, miR-182, miR-200a, mir-210, miR-211, miR-302b
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Neighboring Countries: The Same Professional Aim in Development Laboratory Medicine. J. Med. Biochem. Pub Date : 2019-03-03 Nada Majkić-Singh
During 15th Belgrade Symposium for Balkan Region (April 11 and 12, 2019, Belgrade, www.dmbj.org.rs) Society of Medical Biochemists of Serbia organized scientific and professional program with aim to discuss laboratory medicine topics of mutual interest for all the countries of the Region, such as: Laboratory Medicine Planning and OrganizationType of Medical Laboratory and StrategyLaboratory Medicine
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Biochemical Markers for Prediction of Hypertensive Disorders of Pregnancy. J. Med. Biochem. Pub Date : 2019-03-01 Dušica Kocijančić Belovic,Snežana Plešinac,Jelena Dotlić,Ana Savić Radojević,Slavica Akšam,Mirjana Marjanović Cvjetićanin,Aleksandar Kocijančić
Background Gestational hypertension (GH) and pre eclampsia (PE) are the most common gestational complications. Several placental biochemical markers are used to predict GH/PE, but with conflicting results. Methods The study aim was to estimate the biochemical markers' ability to predict hypertensive disorders in pregnancy. On the first ultrasonographic examination, 104 healthy pregnant women were recruited
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Importance of Copy Number Alterations of FGFR1 and C-MYC Genes in Triple Negative Breast Cancer. J. Med. Biochem. Pub Date : 2019-03-01 Milica Nedeljković,Nikola Tanić,Tatjana Dramićanin,Zorka Milovanović,Snežana Šušnjar,Vedrana Milinković,Ivana Vujović,Mirjana Prvanović,Nasta Tanić
Background Triple negative breast cancer (TNBC) is characterized by aggressive clinical course and is unresponsive to anti-HER2 and endocrine therapy. TNBC is difficult to treat and is often lethal. Given the need to find new targets for therapy we explored clinicopathological significance of copy number gain of FGFR1 and c-MYC. Our aim was to determine the impact of FGFR1 and c-MYC copy number gain
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Relationship of High Circulating Cystatin C to Biochemical Markers of Bone Turnover and Bone Mineral Density in Elderly Males with a Chronic Heart Failure. J. Med. Biochem. Pub Date : 2019-03-01 Biljana Bozic Nedeljkovic,Goran Loncar,Tjasa Vizin,Zoran Radojicic,Vera Popovic Brkic,Janko Kos
Background The aim of the study was to investigate the association of Cystatin C (CysC) to biochemical markers of bone turnover and bone mass, and to evaluate its prognostic significance in elderly males with chronic heart failure (CHF). Methods A prospective cohort study was executed on sixtyeight males (mean age 68±7 years) with mild to moderate CHF, together with 19 of corresponding age- and body
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Significance of UGT1A1*28 Genotype in Patients with Advanced Liver Injury Caused By Chronic Hepatitis C. J. Med. Biochem. Pub Date : 2019-03-01 Jelena Jordovic,Ksenija Bojovic,Jasmina Simonovic-Babic,Vladimir Gasic,Nikola Kotur,Branka Zukic,Marija Vukovic,Sonja Pavlovic,Ivana Lazarevic,Ivana Bekic,Natasa Nikolic,Aleksandar Uroševic,Nikola Mitrovic,Dragan Delic
Background Chronic hepatitis C (CHC) is a significant cause of liver related morbidity and mortality worldwide. The role of genetics in the host response to hepatitis C virus is not elucidated. Genetic variations in UGT1A1 gene are the most common cause of hereditary unconjugated hyperbilirubinemia-Gilbert syndrome. This is the first study investigating the association of UGT1A1 TA repeats promoter
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Novel Mutations in Serbian MEN1 Patients: Genotype-phenotype Correlation. J. Med. Biochem. Pub Date : 2019-03-01 Tatjana Isailovic,Ivana Milicevic,Djuro Macut,Milan Petakov,Sanja Ognjanovic,Bojana Popovic,Ivana Bozic Antic,Tamara Bogavac,Valentina Elezovic Kovacevic,Dusan Ilic,Svetozar Damjanovic
Background Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant cancer syndrome characterized by the occurrence of primary hyperparathyroidism (PHPT), pituitary adenoma (PA) and pancreatic neuroendocrine tumor (pNET). Whether the underlying mutations in MEN1 gene predict clinical presentation of affected heterozygotes or not, is still a matter of a debate. Methods Clinical and genetic
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Evaluation of Analytical Performance of Variant II Turbo HbA1c Analyzer According to Sigma Metrics. J. Med. Biochem. Pub Date : 2019-03-01 Giray Bozkaya,Nuriye Uzuncan,Sibel Bilgili,Ozlem Demirezen
Background Hemoglobin A1c, (HbA1c) which is the major constituent of glycated hemoglobin, has been used in the follow-up of retrospective glycemia for years and in the diagnosis of diabetes mellitus nowadays. Since the analytical performance of HbA1c should be high likewise all laboratory tests, various quality control measures are used. Sigma metrics is one of these measures and it is the combination
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Age and Assay Related Changes of Laboratory Thyroid Function Tests in the Reference Female Population. J. Med. Biochem. Pub Date : 2019-03-01 Najdana Gligorovic Barhanovic,Tanja Antunovic,Sreten Kavaric,Aleksandar Djogo,Vesna Kalimanovska Spasojevic
Background Laboratory thyroid function tests play a central role in the diagnosis of thyroid disorders. The aim of our cross-sectional study was to determine reference values for thyroid tests in a rigorously selected group of Montenegrin females, investigate the impact of possible age-related changes and the influence of the interassay bias between three frequently used immunoassays. Methods Female
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Novel Associations between BRCA1 Variants C.181 T>G (Rs28897672) and Ovarian Crisk in Saudi Females. J. Med. Biochem. Pub Date : 2019-03-01 Nora Alyahri,Saba Abdi,Wajahatullah Khan,Mohamed Elrobh,Mohammed H Addar,Zeneb A Babay,Mohamed Alanazi,Sooad Aldaihan,Jilani Shaik,Maha Arafah,Narasimha Reddy Parine,Arjumand Warsy
Background Mutations in BRCA1 gene have been implicated in ovarian cancers, and BRCA testing may be conducted in high-risk women. This study was designed to determine the frequency of three single nucleotide polymorphisms (SNPs) variants in BRCA1 gene and BRCA1 expression in Saudi females with ovarian cancer. Methods Expression levels of mRNA of BRCA1 gene were studied in 10 ovarian cancer and 10 normal
更新日期:2019-11-01