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Multi-Epitope Vaccines (MEVs), as a Novel Strategy Against Infectious Diseases Curr. Proteom. (IF 0.68) Pub Date : 2020-09-30 Mohammad Mahmoudi Goumari , Ibrahim Farhani, Navid Nezafat and Shirin Mahmoodi
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Comparative iTRAQ Proteomic Analysis Provides the Molecular Basis of the Metabolites in Cultivated Glycyrrhiza uralensis and its Wild-Type Curr. Proteom. (IF 0.68) Pub Date : 2020-09-30 Chengcheng Wang, Lihong Chen , Zhichen Cai , Sijing Feng , Moyi Yue , Jiaojiao Yang , Lisi Zou and Xunhong Liu
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Exploring PLAC1 Structure and Underlying Mechanisms to Design a Derivative Vaccine Against Breast Cancer Progression; In-Silico Study Curr. Proteom. (IF 0.68) Pub Date : 2020-09-30 Farzaneh Afzali, Parisa Ghahremanifard, Mohammad Mehdi Ranjbar and Mahdieh Salimi
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Phosphoproteomic Analysis of Spiroplasma eriocheiris and Crosstalk with Acetylome Reveals the Role of Post-Translational Modifications in Metabolism Curr. Proteom. (IF 0.68) Pub Date : 2020-09-30 Peng Liu, Libo Hou, Min Liu, Xuechuan Xu, Qi Gao, Jiewen Deng, Shasha Xiang, Qian Cao, Min Zhou, Quanjie Yang, Wen Wang, Wei Gu and Qingguo Meng
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Proteomics Analysis of Escherichia coli Treated with Nanosilver: An Approach to Analyze the Bactericidal Action Curr. Proteom. (IF 0.68) Pub Date : 2020-09-30 Nafeesa Khatoon, Hammad Alam and Meryam Sardar
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A Computational Study to Prevent HIV Invasion by Bovine LF in Mucosal-Layer via Blocking of DC-SIGN_GP120 Interaction Curr. Proteom. (IF 0.68) Pub Date : 2020-09-30 Arundhati Banerjee, Rakhi Dasgupta and Sujay Ray
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Inhibition of USP2 Induces Apoptosis through Down Regulation of Fatty Acid Synthase and Cyclin D1 in Triple Negative Breast Cancer Curr. Proteom. (IF 0.68) Pub Date : 2020-09-30 Nida Syed, Amber Ilyas, Farha Idrees, Shamshad Zarina and Zehra Hashim
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Exploration of Mycelial Proteins from Aspergillus terreus Revealed Ribosome Biogenesis and Antioxidant Enzymes Curr. Proteom. (IF 0.68) Pub Date : 2020-09-30 Sonia Kumari Shishodia and Jata Shankar
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Computational Methods for Predicting DNA Binding Proteins Curr. Proteom. (IF 0.68) Pub Date : 2020-07-31 Gaofeng Pan, Jiandong Wang, Liang Zhao, William Hoskins and Jijun Tang
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An Integrated Prediction Method for Identifying Protein-Protein Interactions Curr. Proteom. (IF 0.68) Pub Date : 2020-07-31 Chang Xu, Limin Jiang, Zehua Zhang, Xuyao Yu, Renhai Chen and Junhai Xu
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Identifying Ligand-receptor Interactions via an Integrated Fuzzy Model Curr. Proteom. (IF 0.68) Pub Date : 2020-07-31 Chang Xu, Yijie Ding, Limin Jiang, Cong Shen, Gaoyan Zhang and Xuyao Yu
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Identification of DNA-Binding Proteins by Multiple Kernel Support Vector Machine and Sequence Information Curr. Proteom. (IF 0.68) Pub Date : 2020-07-31 Yijie Ding, Feng Chen, Xiaoyi Guo, Jijun Tang and Hongjie Wu
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Distorted Key Theory and its Implication for Drug Development Curr. Proteom. (IF 0.68) Pub Date : 2020-07-31 Kuo-Chen Chou
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Platelet-Derived Growth Factors (PDGFs) are Key Players in the Stimulation of Airway Smooth Muscle Cells (ASMCs) Alteration in Asthma and Chronic Obstructive Pulmonary Disease (COPD) with Multifarious Inhibitors at an Early Stage of Development Curr. Proteom. (IF 0.68) Pub Date : 2020-07-31 Xiaodong Shi and Kwaku Appiah-Kubi
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In Silico Design of Chimeric and Immunogenic Protein-Containing IpaB and IpaD as a Vaccine Candidate against Shigella dysenteriae Curr. Proteom. (IF 0.68) Pub Date : 2020-07-31 Seyed Akbar Arianzad, Mehdi Zeinoddini, Azam Haddadi, Shahram Nazarian and Reza Hasan Sajedi
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In Silico Identification and Molecular Characterization of Extracellular Cathepsin L Proteases from Giardia duodenalis Curr. Proteom. (IF 0.68) Pub Date : 2020-07-31 Sergio A. Durán-Pérez, José G. Rendón-Maldonado, Lucio de Jesús Hernandez-Diaz, Annete I. Apodaca-Medina, Maribel Jiménez-Edeza and Julio Montes-Avila
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Arecoline Increases the Production of Nitric Oxide and Post-Translational S-Nitrosoproteome in Endothelial Cells Curr. Proteom. (IF 0.68) Pub Date : 2020-05-31 Chien-Yi Wu, Wun-Rong Lin, Cherng-Jye Jeng, Chien-Hsing Wu and Bin Huang
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Upregulated Retinol Binding Protein and Transthyretin as Predictive Biomarkers of Gestational Diabetes Mellitus Curr. Proteom. (IF 0.68) Pub Date : 2020-05-31 Sadaf Ilyas, Nabila Roohi, Samina Ashraf and Sobia Alyas
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Chemoprotective Effects of Propolis on Aflatoxin B1-Induced Hepatotoxicity in Rats: Oxidative Damage and Hepatotoxicity by Modulating TP53, Oxidative Stress Curr. Proteom. (IF 0.68) Pub Date : 2020-05-31 Seval Yilmaz, Fatih Mehmet Kandemir, Emre Kaya and Mustafa Ozkaraca
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Analyzing Structural and Functional Characteristics of Collagenase from Bacillus cereus MH19 via in silico Approaches Curr. Proteom. (IF 0.68) Pub Date : 2020-05-31 Shihua Chen, Meihu Ma and Xing Fu
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In Silico Evaluation of the ATP7B Protein: Insights from the Role of Rare Codon Clusters and Mutations that Affect Protein Structure and Function Curr. Proteom. (IF 0.68) Pub Date : 2020-05-31 Mojtaba Mortazavi , Abdolrazagh Barzegar , Abdorrasoul Malekpour, Mohammad Ghorbani, Saeid Gholamzadeh and Younes Ghasemi
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Identification of Drought-Responsive Proteins of Sensitive and Tolerant Tea (Camellia sinensis L) Clones under Normal and Drought Stress Conditions Curr. Proteom. (IF 0.68) Pub Date : 2020-05-31 Mehdi Rahimi, Mojtaba Kordrostami, Mojtaba Mortezavi and Sanam SafaeiChaeikar
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Characterization of Venoms of Deinagkistrodon acutus and Bungarus multicinctus Using Proteomics and Peptidomics Curr. Proteom. (IF 0.68) Pub Date : 2020-05-31 Yaqiong Zhang, Zhiping Jia, Yunyang Liu, Xinwen Zhou and Yi Kong
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Antibody Development to HCV Alternate Reading Frame Protein in Liver Transplant Candidate and its Computational Analysis Curr. Proteom. (IF 0.68) Pub Date : 2020-03-31 Zahra Musavi, Tayebeh Hashempour, Javad Moayedi, Behzad Dehghani, Farzaneh Ghassabi, Mehrdad Hallaji, Seyed Younes Hosseini, Ramin Yaghoubi , Siavash Gholami, Mohamad Ali Dehyadegari and Shahin Merat
Background: HCV Alternate Reading Frame Protein (ARFP) is a frameshift product of HCV-core encoding. Here, we characterized specific anti-ARFP antibodies in Liver Transplant Candidate (LTC) and chronic HCV-infected patients. Methods: The ARFP gene was cloned and the recombinant protein was purified using Nickel chromatography and confirmed by western blotting. ELISA was developed using recombinant
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Proteomics Study in Urolithiasis Curr. Proteom. (IF 0.68) Pub Date : 2020-03-31 Manavi Jain, Paramveer Yadav and Priyadarshini
Urolithiasis, which is the presence of stones in the urinary tract, has long been linked with a higher risk of causing chronic kidney diseases and associated illnesses, such as diabetes-affecting 12% of the world population. This clinical condition arises due to the supersaturation of urine and alterations in the expression of cellular and urinary proteins. The renal stone mineral composition has been
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Computational Analysis of the Primary and Secondary Structure of Amidases in Relation to their pH Adaptation Curr. Proteom. (IF 0.68) Pub Date : 2020-03-31 Neerja Thakur, Nikhil Sharma, Vijay Kumar and Tek Chand Bhalla
Background: Amidases are ubiquitous enzymes and biological functions of these enzymes vary widely. They are considered to be synergistically involved in the synthesis of a wide variety of carboxylic acids, hydroxamic acids and hydrazides, which find applications in commodity chemicals synthesis, pharmaceuticals agrochemicals and wastewater treatments. Methods: They hydrolyse a wide variety of amides
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Interaction of Human Herpesvirus 8 Viral Interleukin-6 with Human Interleukin-6 Receptor Using In Silico Approach: The Potential Role in HHV-8 Pathogenesis Curr. Proteom. (IF 0.68) Pub Date : 2020-03-31 Behzad Dehghani, Tayebeh Hashempour and Zahra Hasanshahi
Introduction: Human Herpesvirus 8 (HHV-8) causes classical, endemic (African), and Acquired Immunodeficiency Syndrome (AIDS)-related Kaposi’s Sarcoma (KS), Body Cavity-Based Primary Effusion Lymphomas (BCBL), HHV-8-associated peritoneal Primary Effusion Lymphoma (PEL), and Multicentric Castleman’s Disease (MCD). HHV8 genome encodes several structural and non-structural proteins, among which vIL6 is
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Genetic and Epigenetic Factors of E3/E3 Genotypes of APO-E Gene as a Strong Predictor for the Diagnosis of Coronary Artery Disease Patients of South India Curr. Proteom. (IF 0.68) Pub Date : 2020-03-31 Javeed Ahmad Tantray , Karnati Pratap Reddy , Kaiser Jamil , Waseem Gul Lone and Shiva Kumar Yerra
Background: The role of Apolipoprotein-E (APO-E) in lipid metabolism and cholesterol transport is a key component of lipid metabolism which plays a role in diseases like hypercholesterolemia, diabetes, and cardiovascular disease. The aim of this study was to determine the genotypes, allelic frequencies, gene expression and methylation related to apolipoprotein E polymorphism in Coronary Artery Disease
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Computational Analysis of Arginine Deiminase Sequences to Provide a Guideline for Protein Engineering Curr. Proteom. (IF 0.68) Pub Date : 2020-03-31 Mahboubeh Zarei, Mohammad Reza Rahbar, Navid Nezafat, Manica Negahdaripour, Mohammad Hossein Morowvat and Younes Ghasemi
Background: Arginine deiminase of Mycoplasma hominis, an arginine catabolizing enzyme, is currently in clinical trial for the treatment of arginine auxotrophic cancers. However, some drawbacks such as instability and antigenicity have limited its application as a protein drug. Arginine Deiminase (ADI) belongs to the guanidino-group modifying enzyme superfamily. Despite differences in the primary amino
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Cell Penetrating Peptide: Sequence-Based Computational Prediction for Intercellular Delivery of Arginine Deiminase Curr. Proteom. (IF 0.68) Pub Date : 2020-03-31 Mahboubeh Zarei, Mohammad Reza Rahbar, Manica Negahdaripour, Mohammad Hossein Morowvat, Navid Nezafat and Younes Ghasemi
Background: Cell-Penetrating Peptides (CPPs), a family of short peptides, are broadly used as the carrier in the delivery of drugs and different therapeutic agents. Thanks to the existence of valuable databases, computational screening of the experimentally validated CPPs can help the researchers to select more effective CPPs for the intercellular delivery of therapeutic proteins. Arginine deiminase
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In Silico Study of 1, 4 Alpha Glucan Branching Enzyme and Substrate Docking Studies Curr. Proteom. (IF 0.68) Pub Date : 2020-01-31 Farzane Kargar, Amir Savardashtaki, Mojtaba Mortazavi, Masoud Torkzadeh Mahani, Ali Mohammad Amani, Younes Ghasemi and Navid Nezafat
Background: The 1,4-alpha-glucan branching protein (GlgB) plays an important role in the glycogen biosynthesis and the deficiency in this enzyme has resulted in Glycogen storage disease and accumulation of an amylopectin-like polysaccharide. Consequently, this enzyme was considered a special topic in clinical and biotechnological research. One of the newly introduced GlgB belongs to the Neisseria sp
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Evaluation of Luciferase Thermal Stability by Arginine Saturation in the Flexible Loops Curr. Proteom. (IF 0.68) Pub Date : 2020-01-31 Farzane Kargar, Mojtaba Mortazavi, Masoud Torkzadeh-Mahani, Safa Lotfi and Shahryar Shakeri
Background: The firefly luciferase enzyme is widely used in protein engineering and diverse areas of biotechnology, but the main problem with this enzyme is low-temperature stability. Previous reports indicated that surface areas of thermostable proteins are rich in arginine, which increased their thermal stability. In this study, this aspect of thermophilic proteins evaluated by mutations of surface
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Varlitinib Mediates Its Activity Through Down Regulating MAPK/EGFR Pathway in Oral Cancer Curr. Proteom. (IF 0.68) Pub Date : 2020-01-31 Muhammad Usman, Fariha Tanveer, Amber Ilyas and Shamshad Zarina
Background: Oral Squamous Cell Carcinoma (OSCC) is a major sub-type of oral cancer that shares 90% proportion of oral cavity cancers. It is declared as the sixth most frequent cancer among all cancer types throughout the world. Higher morbidity in Asian countries is reported due to frequent use of Smokeless Tobacco (SLT) products besides exposure to other risk factors. Hyperactivation of epidermal
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Computational Analysis of Therapeutic Enzyme Uricase from Different Source Organisms Curr. Proteom. (IF 0.68) Pub Date : 2020-01-31 Anand Kumar Nelapati and JagadeeshBabu PonnanEttiyappan
Background: Hyperuricemia and gout are the conditions, which is a response of accumulation of uric acid in the blood and urine. Uric acid is the product of purine metabolic pathway in humans. Uricase is a therapeutic enzyme that can enzymatically reduces the concentration of uric acid in serum and urine into more a soluble allantoin. Uricases are widely available in several sources like bacteria, fungi
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Analysis of Extracellular Proteome of Staphylococcus aureus: A Mass Spectrometry based Proteomics Method of Exotoxin Characterisation Curr. Proteom. (IF 0.68) Pub Date : 2020-01-31 Rajdeep Das, Nisha D`souza, Surya K. Choubey, Sethumadhavan Murlidharan, Anura V. Kurpad and Amit K. Mandal
Background: Staphylococcus aureus (S. aureus), an important pathogen, causes a wide range of infections in human starting from food poisoning to septicemia. It affects the host cells with various exotoxins, known as virulence factors, which are synthesized in growth phase-dependent manner of the bacteria. S. aureus has been reported to become resistant to antibiotics rapidly. Among two common clinical
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In silico Evaluation of Substrate Binding Site and Rare Codons in the Structure of CYP152A1 Curr. Proteom. (IF 0.68) Pub Date : 2020-01-31 Mojtaba Mortazavi, Navid Nezafat, Manica Negahdaripour, Mohammad J. Raee, Masoud Torkzadeh-Mahani, Ali Riahi-Madvar and Younes Ghasemi
Background: The Cytochromes P450 (CYPs) have an essential role in the oxidation of endogenous and exogenous molecules. The CYPs are identified in all domains of life, but the CYP152A1 from Bacillus subtilis is specially considered for clinical and industrial applications. The molecular cloning of a new type of CYP from Bacillus subtilis was reported, previously. Here, we describe the hidden layer of
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Role of Cervical Cancer Radiotherapy in the Expression of EGFR and p53 Gene Curr. Proteom. (IF 0.68) Pub Date : 2020-01-31 Yan Cheng, Kuntian Lan, Xiaoxia Yang, Dongxia Liang, Li Xia and Jinquan Cui
Background: Cervical cancer arises from the cervix and it is the 3rd most diagnosed malignancy and a foremost cause of cancer-related death in females. On the other hand, the expressions of EGFR and p53 are two important proteins observed in various studies on cervical cancer. Objective: The study aims to evaluate the beneficial effect of radiotherapy based on the regulation of p53 and EGFR gene in
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Structural and Functional Analysis of Mutated Human Pyrin B30.2 Domain Curr. Proteom. (IF 0.68) Pub Date : 2020-01-31 Sepideh Parvizpour, Ashraf Fadhil Jomah and Jafar Razmara
Background: Familial Mediterranean Fever (FMF) is a prototypical hereditary autoinflammatory disease affecting principally Mediterranean populations and characterized by recurrent frequent fever and inflammation. The disease is essentially caused by inherited mutations in the MEFV gene which encodes pyrin protein. The reported mutations are mostly located on the B30.2 domain in the C-terminal end of
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ML-rRBF-ECOC: A Multi-Label Learning Classifier for Predicting Protein Subcellular Localization with Both Single and Multiple Sites Curr. Proteom. (IF 0.68) Pub Date : 2019-09-30 Guo-Sheng Han and Zu-Guo Yu
Background: The subcellular localization of a protein is closely related with its functions and interactions. More and more evidences show that proteins may simultaneously exist at, or move between, two or more different subcellular localizations. Therefore, predicting protein subcellular localization is an important but challenging problem. Observation: Most of the existing methods for predicting
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The Applications of Clustering Methods in Predicting Protein Functions Curr. Proteom. (IF 0.68) Pub Date : 2019-09-30 Weiyang Chen, Weiwei Li, Guohua Huang and Matthew Flavel
Background: The understanding of protein function is essential to the study of biological processes. However, the prediction of protein function has been a difficult task for bioinformatics to overcome. This has resulted in many scholars focusing on the development of computational methods to address this problem. Objective: In this review, we introduce the recently developed computational methods
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Pathogenic Genes Selection Model of Genetic Disease based on Network Motifs Slicing Feedback Curr. Proteom. (IF 0.68) Pub Date : 2019-09-30 Shengli Zhang, Zekun Tong, Haoyu Yin and Yifan Feng
Background: Finding the pathogenic gene is very important for understanding the pathogenesis of the disease, locating effective drug targets and improving the clinical level of medical treatment. However, the existing methods for finding the pathogenic genes still have limitations, for instance the computational complexity is high, and the combination of multiple genes and pathways has not been considered
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A Novel Gene Selection Algorithm based on Sparse Representation and Minimum-redundancy Maximum-relevancy of Maximum Compatibility Center Curr. Proteom. (IF 0.68) Pub Date : 2019-09-30 Min Chen, Yi Zhang, Zejun Li, Ang Li, Wenhua Liu, Liubin Liu and Zheng Chen
Background: Tumor classification is important for accurate diagnosis and personalized treatment and has recently received great attention. Analysis of gene expression profile has shown relevant biological significance and thus has become a research hotspot and a new challenge for bio-data mining. In the research methods, some algorithms can identify few genes but with great time complexity, some algorithms
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A Method for Analyzing Two-locus Epistasis of Complex Diseases based on Decision Tree and Mutual Entropy Curr. Proteom. (IF 0.68) Pub Date : 2019-09-30 Xiong Li, Hui Yang, Kaifu Wen, Xiaoming Zhong, Xuewen Xia, Liyue Liu and Dehao Qin
Background: Epistasis makes complex diseases difficult to understand, especially when heterogeneity also exists. Heterogeneity of complex diseases makes the distribution of case population more confused. However, the traditional methods proposed to detect epistasis often ignore heterogeneity, resulting in low power of association studies. Methods: In this study, we firstly use rank information in the
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A Binary Classifier for the Prediction of EC Numbers of Enzymes Curr. Proteom. (IF 0.68) Pub Date : 2019-09-30 Hao Cui and Lei Chen
Background: Identification of Enzyme Commission (EC) number of enzymes is quite important for understanding the metabolic processes that produce enough energy to sustain life. Previous studies mainly focused on predicting six main functional classes or sub-functional classes, i.e., the first two digits of the EC number. Objective: In this study, a binary classifier was proposed to identify the full
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Protein Subcellular Localization Prediction based on PSI-BLAST Profile and Principal Component Analysis Curr. Proteom. (IF 0.68) Pub Date : 2019-09-30 Yuhua Yao, Manzhi Li, Huimin Xu, Shoujiang Yan, Pingan He, Qi Dai, Zhaohui Qi and Bo Liao
Background: Prediction of protein subcellular location is a meaningful task which attracts much attention in recent years. Particularly, the number of new protein sequences yielded by the highthroughput sequencing technology in the post genomic era has increased explosively. Objective: Protein subcellular localization prediction based solely on sequence data remains to be a challenging problem of computational
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Identification of Novel Breast Cancer Genes based on Gene Expression Profiles and PPI Data Curr. Proteom. (IF 0.68) Pub Date : 2019-09-30 Cheng-Wen Yang, Huan-Huan Cao, Yu Guo, Yuan-Ming Feng and Ning Zhang
Background: Breast cancer is one of the most common malignancies, and a threat to female health all over the world. However, the molecular mechanism of breast cancer has not been fully discovered yet. Objective: It is crucial to identify breast cancer-related genes, which could provide new biomarker for breast cancer diagnosis as well as potential treatment targets. Methods: Here we used the minimum
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An Improved Scatter Search Algorithm for Parameter Estimation in Large-Scale Kinetic Models of Biochemical Systems Curr. Proteom. (IF 0.68) Pub Date : 2019-09-30 Muhammad Akmal Remli, Mohd Saberi Mohamad, Safaai Deris, Richard Sinnott and Suhaimi Napis
Background: Mathematical models play a central role in facilitating researchers to better understand and comprehensively analyze various processes in biochemical systems. Their usage is beneficial in metabolic engineering as they help predict and improve desired products. However, one of the primary challenges in model building is parameter estimation. It is the process to find nearoptimal values of
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Upregulation of Cathepsin B-like Protease Activity During Apoptosis in Giardia duodenalis Curr. Proteom. (IF 0.68) Pub Date : 2019-07-31 Sergio Alonso Durán-Pérez, Héctor Samuel López-Moreno, Maribel Jiménez-Edeza, Jesús Ricardo Parra-Unda, Edgar Rangel-López and José Guadalupe Rendón-Maldonado
Background: In eukaryotic cells, apoptosis signaling pathways are controlled mainly by aspartic acid cysteine proteases (caspases). However, certain unicellular microorganisms, such as Giardia duodenalis, lack these proteins. Thus, other cysteine proteases may play an important role in the parasite apoptosis signaling pathway. Objective: To understand the effect of cathepsin B-like inhibition on the
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Fibrinolytic Activity of Two Polypeptide Chains from Human Plasminogen# Curr. Proteom. (IF 0.68) Pub Date : 2019-07-31 Agustín Joison, Gustavo Baiardi, Rocío Donalisio and Federico Gallo
Background: Plasminogen is a blood plasma glycoprotein of molecular weight about 92,000 Daltons. Physiologically, it incorporates into blood clots and after its activation by plasminogen activators to plasmin can perform a fibrinolytic function. Microplasmin is truncate polypeptide chain derivate of plasmin may be increase the fibrinolytic activity. Objective: To study the amino acid sequence of two
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An iTRAQ Based Comparative Proteomic Profiling of Thermotolerant Saccharomyces cerevisiae JRC6 in Response to High Temperature Fermentation Curr. Proteom. (IF 0.68) Pub Date : 2019-07-31 Jairam Choudhary, Surender Singh, Rameshwar Tiwari, Renu Goel and Lata Nain
Background: Bioethanol derived from lignocellulosic biomass can supplement the ethanol supplies in a sustainable manner. However, the bioethanol production process is still not cost effective and researchers are looking for novel strategies like simultaneous saccharification fermentation to cut down the production cost. Thermotolerant yeast Saccharomyces cerevisiae JRC6 is reported to improve the fermentation
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In Silico and in Vitro Evaluation of Deamidation Effects on the Stability of the Fusion Toxin DAB389IL-2 Curr. Proteom. (IF 0.68) Pub Date : 2019-07-31 Nasrin Zarkar, Mohammad Ali Nasiri Khalili, Fathollah Ahmadpour, Sirus Khodadadi and Mehdi Zeinoddini
Background: DAB389IL-2 (Denileukin diftitox) as an immunotoxin is a targeted pharmaceutical protein and is the first immunotoxin approved by FDA. It is used for the treatment of various kinds of cancer such as CTCL lymphoma, melanoma, and Leukemia but among all of these, treatment of CTCL has special importance. DAB389IL-2 consists of two distinct parts; the catalytic domain of Diphtheria Toxin (DT)
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Mass Spectrometry for Proteomics and Recent Developments in ESI, MALDI and other Ionization Methodologies Curr. Proteom. (IF 0.68) Pub Date : 2019-07-31 Qurat ul Ain Farooq, Noor ul Haq, Abdul Aziz, Sara Aimen and Muhammad Inam ul Haq
Background: Mass spectrometry is a tool used in analytical chemistry to identify components in a chemical compound and it is of tremendous importance in the field of biology for high throughput analysis of biomolecules, among which protein is of great interest. Objective: Advancement in proteomics based on mass spectrometry has led the way to quantify multiple protein complexes, and proteins interactions
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Proteomics Study of Mesenchymal Stem Cell-Like Cells Isolated from Cerebrospinal Fluid of Patients with Meningioma Curr. Proteom. (IF 0.68) Pub Date : 2019-07-31 Arash Saffarian, Amir Tarokh, Mohammad Reza Haghshenas, Mousa Taghipour, Nooshafarin Chenari, Abbas Ghaderi and Mahboobeh Razmkhah
Background: Cerebrospinal fluid (CSF) contains pro-growth factors that can affect proliferation, migration and differentiation of Mesenchymal Stem Cells (MSCs). Objective: This study aimed to isolate MSC like cells from CSF of patients with meningioma and psudotumorcerebri (PTC) and identify differentially expressed proteins in these cells. Methods: Five patients with newly diagnosed intracranial meningioma
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Comparative iTRAQ Proteomics Identified Myocardium Proteins Associated with Hypoxia of Yak Curr. Proteom. (IF 0.68) Pub Date : 2019-07-31 Asma Babar, Tserang Donko Mipam, Shixin Wu, Chuanfei Xu, Mujahid Ali Shah, Kifayatullah Mengal, Chuanping Yi, Hui Luo, Wangsheng Zhao, Xin Cai and Xuegang Luo
Background: Yaks inhabit high-altitude are well-adapted to the hypoxic environments. Though, the mechanisms involved in regulatory myocardial protein expression at high-altitude were not completely understood. Objective: To revel the molecular mechanism of hypoxic adaptation in yak, here we have applied comparative myocardial proteomics in between yak and cattle by isobaric Tag for Relative and Absolute
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The Combination of the CIGB-300 Anticancer Peptide and Cisplatin Modulates Proteins Related to Cell Survival, DNA Repair and Metastasis in a Lung Cancer Cell Line Model Curr. Proteom. (IF 0.68) Pub Date : 2019-07-31 Arielis Rodríguez-Ulloa, Yassel Ramos, Aniel Sánchez-Puente, Yasser Perera, Alexis Musacchio-Lasa , Jorge Fernández-de-Cossio, Gabriel Padrón, Luis J.G. López, Vladimir Besada and Silvio E. Perea
Background: CIGB-300 is a pro-apoptotic peptide that abrogates CK2-mediated phosphorylation, and can elicit synergistic interaction in vitro and in vivo when combined with certain anticancer drugs. Objective: The combination of CIGB-300 with cisplatin is studied through data mining and expressionbased proteomics to reveal the molecular basis of this interaction. Cisplatin resistance-associated proteins
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Identification of the Altered Proteins Related to Colon Carcinogenesis by iTRAQ-based Quantitative Proteomic Analysis Curr. Proteom. (IF 0.68) Pub Date : 2019-07-31 Chunhua Luo, Defu Yao, Teck Kwang Lim, Qingsong Lin and Yingfu Liu
Background: The molecular mechanisms or valuable biomarkers for early diagnosis of colorectal cancer (CRC) are not fully elucidated yet. Objective: To understand the proteomic changes at the global level in the carcinogenesis of CRC, differentially expressed proteins between normal intestinal epithelial cells CCD841 and colorectal cancer cells HCT116 were identified. Method: The isobaric tags for relative
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Neuroproteomics in Paving the Pathway for Drug Abuse Research Curr. Proteom. (IF 0.68) Pub Date : 2019-07-31 Muhammad Naveed, Attha Tallat, Ayesha Butt, Maria Khalid, Marium Shehzadi, Nida Bashir, Komal Kafait Ullah Malik, Shafia Tufail and Faisal Nouroz
Neuroproteomics, as a sub-discipline of proteomics, has enlightened the pathway for the study of different complicated diseases and brain disorders. Since four decades, various analytical and quantitative techniques have been used to cure problems related to brain and memory. Brain has a complex structure with various cells and cell types, the expressing proteins and suppressing factors too. Drug addiction
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Effect of Intestinal Flora Clearance on Liver Proteomics in Mice Curr. Proteom. (IF 0.68) Pub Date : 2019-05-31 Zhenghu Jia, Hui Liu, Mei Song, Chengmao Yang, Yapu Zhao, Xiaoli Wu, Zhenzhou Wu and Liqing Zhao
Background: Intestinal flora dynamically affects the host's systemic immune system. Liver is one of the organs that may be affected by intestinal microbiota. Materials and Methods: In this study, we aimed to identify proteome level differences between liver tissue from mice cleared intestinal flora and control using tandem mass spectrometry (LC-MS/MS) and label free quantification. Additionally, protein-protein
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Light Chain LC and TAT-EGFP-HCS of Botulinum Toxin Expression and Biological Function in vitro and in vivo Curr. Proteom. (IF 0.68) Pub Date : 2019-05-31 Fengjin Hao, Yueqin Feng and Yifu Guan
Objective: To verify whether the botulinum toxin heavy chain HCS has specific neuronal targeting function and to confirm whether TAT-EGFP-LC has hydrolyzable SNAP-25 and has transmembrane biological activity. Methods: We constructed the pET-28a-TAT-EGFP-HCS/LC plasmid. After the plasmid is expressed and purified, we co-cultured it with nerve cells or tumors. In addition, we used Western-Blot to identify
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iTRAQ-Based Quantitative Proteomic Analysis of Pseudostellaria heterophylla from Geo-Authentic Habitat and Cultivated Bases Curr. Proteom. (IF 0.68) Pub Date : 2019-05-31 Yujiao Hua, Chengcheng Wang, Shengnan Wang, Zixiu Liu, Xunhong Liu, Lisi Zou, Wei Gu, Yiyuan Luo and Juanxiu Liu
Background: Pseudostellaria heterophylla is an important tonic traditional Chinese medicine. However, the molecular changes in the herb from geo-authentic habitat and cultivated bases remain to be explored. Objective: The purpose of this research was to study differences in P. heterophylla from geo-authentic habitat and cultivated bases. Methods: High-throughput technologies of transcriptomic and proteomic