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Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling Stem. Cell Rep. (IF 5.9) Pub Date : 2024-02-29 Satoshi Yamazaki, Yo Mabuchi, Takaharu Kimura, Eriko Grace Suto, Daisuke Hisamatsu, Yuna Naraoka, Ayako Kondo, Yuzuki Azuma, Riko Kikuchi, Hidekazu Nishikii, Soji Morishita, Marito Araki, Norio Komatsu, Chihiro Akazawa
Myeloid cells, which originate from hematopoietic stem/progenitor cells (HSPCs), play a crucial role in mitigating infections. This study aimed to explore the impact of mesenchymal stem/stromal cells (MSCs) on the differentiation of HSPCs and progenitors through the C-C motif chemokine CCL2/CCR2 signaling pathway. Murine MSCs, identified as PDGFRαSca-1 cells (PαS cells), were found to secrete CCL2
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MSX1+PDGFRAlow limb mesenchyme-like cells as an efficient stem cell source for human cartilage regeneration Stem. Cell Rep. (IF 5.9) Pub Date : 2024-02-29 Yuansong Liao, Fanchen Kang, Jingfei Xiong, Kun Xie, Mingxu Li, Ling Yu, Yuqing Wang, Hanyi Chen, Guogen Ye, Yike Yin, Weihua Guo, Haoyang Cai, Qing Zhu, Zhonghan Li
Degenerative bone disorders have a significant impact on global health, and regeneration of articular cartilage remains a challenge. Existing cell therapies using mesenchymal stromal cells (MSCs) have shown limited efficacy, highlighting the necessity for alternative stem cell sources. Here, we have identified and characterized MSX1 mesenchymal progenitor cells in the developing limb bud with remarkable
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Mitochondrial DNA integrity and metabolome profile are preserved in the human induced pluripotent stem cell reference line KOLF2.1J Stem. Cell Rep. (IF 5.9) Pub Date : 2024-02-22 Jochen Dobner, Thach Nguyen, Andreas Dunkel, Alessandro Prigione, Jean Krutmann, Andrea Rossi
Quality control of human induced pluripotent stem cells (iPSCs) is critical to ensure reproducibility of research. Recently, KOLF2.1J was characterized and published as a male iPSC reference line to study neurological disorders. Emerging evidence suggests potential negative effects of mtDNA mutations, but its integrity was not analyzed in the original publication. To assess mtDNA integrity, we conducted
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Human-induced pluripotent stem cell-derived neural stem/progenitor cell ex vivo gene therapy with synaptic organizer CPTX for spinal cord injury Stem. Cell Rep. (IF 5.9) Pub Date : 2024-02-15 Yusuke Saijo, Narihito Nagoshi, Momotaro Kawai, Takahiro Kitagawa, Yu Suematsu, Masahiro Ozaki, Munehisa Shinozaki, Jun Kohyama, Shinsuke Shibata, Kosei Takeuchi, Masaya Nakamura, Michisuke Yuzaki, Hideyuki Okano
The transplantation of neural stem/progenitor cells (NS/PCs) derived from human induced pluripotent stem cells (hiPSCs) has shown promise in spinal cord injury (SCI) model animals. Establishing a functional synaptic connection between the transplanted and host neurons is crucial for motor function recovery. To boost therapeutic outcomes, we developed an gene therapy aimed at promoting synapse formation
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Growth/differentiation factor 15 controls ependymal and stem cell number in the V-SVZ Stem. Cell Rep. (IF 5.9) Pub Date : 2024-02-15 Katja Baur, Carmen Carrillo-García, Şeydanur Şan, Manja von Hahn, Jens Strelau, Gabriele Hölzl-Wenig, Claudia Mandl, Francesca Ciccolini
The expression of growth/differentiation factor (GDF) 15 increases in the ganglionic eminence (GE) late in neural development, especially in neural stem cells (NSCs). However, GDF15 function in this region remains unknown. We report that GDF15 receptor is expressed apically in the GE and that GDF15 ablation promotes proliferation and cell division in the embryonic GE and in the adult ventricular-subventricular
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Misidentification of neural cell identity in liver-derived organoid systems Stem. Cell Rep. (IF 5.9) Pub Date : 2024-02-08 Imre F. Schene, Arif I. Ardisasmita, Sabine A. Fuchs
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Give heart cells a beat: An interactive museum exhibit that synchronizes stem cell–derived cardiomyocytes to visitors’ heartbeat Stem. Cell Rep. (IF 5.9) Pub Date : 2024-02-08 Juan A. Perez-Bermejo, Samuel J. Reisman, Joyce Ma, Dana Carrison-Stone, Chris Cerrito, Alexandre J.S. Ribeiro, Bruce R. Conklin, Kristina Yu
Science museums play an important role in science education, engaging the public with science concepts and building support for scientific research. Here, we describe Give Heart Cells a Beat, an interactive exhibit that lets museum visitors synchronize the beating of live stem cell–derived cardiomyocytes to their own heart rate in real time. The beat rate of cells accurately matched the beat rate of
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Human iPSC-derived photoreceptor transplantation in the cone dominant 13-lined ground squirrel Stem. Cell Rep. (IF 5.9) Pub Date : 2024-02-08 Ching Tzu Yu, Sangeetha Kandoi, Ramesh Periasamy, L. Vinod K. Reddy, Hannah M. Follett, Phyllis Summerfelt, Cassandra Martinez, Chloe Guillaume, Owen Bowie, Thomas B. Connor, Daniel M. Lipinski, Kenneth P. Allen, Dana K. Merriman, Joseph Carroll, Deepak A. Lamba
Several retinal degenerations affect the human central retina, which is primarily comprised of cones and is essential for high acuity and color vision. Transplanting cone photoreceptors is a promising strategy to replace degenerated cones in this region. Although this approach has been investigated in a handful of animal models, commonly used rodent models lack a cone-rich region and larger models
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ALS-related p97 R155H mutation disrupts lysophagy in iPSC-derived motor neurons Stem. Cell Rep. (IF 5.9) Pub Date : 2024-02-08 Jacob A. Klickstein, Michelle A. Johnson, Pantelis Antonoudiou, Jamie Maguire, Joao A. Paulo, Steve P. Gygi, Chris Weihl, Malavika Raman
Mutations in the AAA+ ATPase cause multisystem proteinopathy 1, which includes amyotrophic lateral sclerosis; however, the pathogenic mechanisms that contribute to motor neuron loss remain obscure. Here, we use two induced pluripotent stem cell models differentiated into spinal motor neurons to investigate how mutations perturb the motor neuron proteome. Using quantitative proteomics, we find that
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ER stress and lipid imbalance drive diabetic embryonic cardiomyopathy in an organoid model of human heart development Stem. Cell Rep. (IF 5.9) Pub Date : 2024-02-08 Aleksandra Kostina, Yonatan R. Lewis-Israeli, Mishref Abdelhamid, Mitchell A. Gabalski, Artem Kiselev, Brett D. Volmert, Haley Lankerd, Amanda R. Huang, Aaron H. Wasserman, Todd Lydic, Christina Chan, Sangbum Park, Isoken Olomu, Aitor Aguirre
Congenital heart defects are the most prevalent human birth defects, and their incidence is exacerbated by maternal health conditions, such as diabetes during the first trimester (pregestational diabetes). Our understanding of the pathology of these disorders is hindered by a lack of human models and the inaccessibility of embryonic tissue. Using an advanced human heart organoid system, we simulated
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The LRRK2 kinase substrates RAB8a and RAB10 contribute complementary but distinct disease-relevant phenotypes in human neurons Stem. Cell Rep. (IF 5.9) Pub Date : 2024-02-01 Adamantios Mamais, Anwesha Sanyal, Austin Fajfer, Catherine G. Zykoski, Michael Guldin, Alexis Riley-DiPaolo, Nitya Subrahmanian, Whitney Gibbs, Steven Lin, Matthew J. LaVoie
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DeePhys: A machine learning–assisted platform for electrophysiological phenotyping of human neuronal networks Stem. Cell Rep. (IF 5.9) Pub Date : 2024-01-25 Philipp Hornauer, Gustavo Prack, Nadia Anastasi, Silvia Ronchi, Taehoon Kim, Christian Donner, Michele Fiscella, Karsten Borgwardt, Verdon Taylor, Ravi Jagasia, Damian Roqueiro, Andreas Hierlemann, Manuel Schröter
Reproducible functional assays to study in vitro neuronal networks represent an important cornerstone in the quest to develop physiologically relevant cellular models of human diseases. Here, we introduce DeePhys, a MATLAB-based analysis tool for data-driven functional phenotyping of in vitro neuronal cultures recorded by high-density microelectrode arrays. DeePhys is a modular workflow that offers
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Searching for information about stem cells online in an age of artificial intelligence: How should the stem cell community respond? Stem. Cell Rep. (IF 5.9) Pub Date : 2024-01-25 Klaus Hoeyer, Anna Couturier, Kali Barawi, Cheney Drew, Anders Grundtvig, Emma Lane, Anders Kristian Munk, Louise Emma Whiteley, Megan Munsie
Patients and their families routinely use the Internet to learn about stem cell research. What they find, is increasingly influenced by ongoing changes in how information is filtered and presented online. This article reflects on recent developments in generative artificial intelligence and how the stem cell community should respond.
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Proinflammatory phenotype of iPS cell-derived JAK2 V617F megakaryocytes induces fibrosis in 3D in vitro bone marrow niche Stem. Cell Rep. (IF 5.9) Pub Date : 2024-01-25 Niclas Flosdorf, Janik Böhnke, Marcelo A.S. de Toledo, Niklas Lutterbach, Vanesa Gómez Lerma, Martin Graßhoff, Kathrin Olschok, Siddharth Gupta, Vithurithra Tharmapalan, Susanne Schmitz, Katrin Götz, Herdit M. Schüler, Angela Maurer, Stephanie Sontag, Caroline Küstermann, Kristin Seré, Wolfgang Wagner, Ivan G. Costa, Tim H. Brümmendorf, Steffen Koschmieder, Martin Zenke
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The impact of timing and injury mode on induced neurogenesis in the adult mammalian retina Stem. Cell Rep. (IF 5.9) Pub Date : 2024-01-25 Marina Pavlou, Marlene Probst, Nicolai Blasdel, Aric R. Prieve, Thomas A. Reh
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Mutations in FUS lead to synaptic dysregulation in ALS-iPSC derived neurons Stem. Cell Rep. (IF 5.9) Pub Date : 2024-01-18 Carole Shum, Erin C. Hedges, Joseph Allison, Youn-bok Lee, Natalia Arias, Graham Cocks, Siddharthan Chandran, Marc-David Ruepp, Christopher E. Shaw, Agnes L. Nishimura
Amyotrophic lateral sclerosis (ALS) is a fatal, adult-onset neurodegenerative disorder characterized by progressive muscular weakness due to the selective loss of motor neurons. Mutations in the gene Fused in Sarcoma (FUS) were identified as one cause of ALS. Here, we report that mutations in FUS lead to upregulation of synaptic proteins, increasing synaptic activity and abnormal release of vesicles
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Autophagy is essential for human myelopoiesis Stem. Cell Rep. (IF 5.9) Pub Date : 2024-01-11 Jiaming Gu, Yanling Zhu, Huaisong Lin, Yuhua Huang, Yanqi Zhang, Qi Xing, Baoqiang Kang, Zhishuai Zhang, Mingquan Wang, Tiancheng Zhou, Yuchan Mai, Qianyu Chen, Fei Li, Xing Hu, Shuoting Wang, Jiaojiao Peng, Xinrui Guo, Bing Long, Junwei Wang, Minghui Gao, Guangjin Pan
Emergency myelopoiesis (EM) is essential in immune defense against pathogens for rapid replenishing of mature myeloid cells. During the EM process, a rapid cell-cycle switch from the quiescent hematopoietic stem cells (HSCs) to highly proliferative myeloid progenitors (MPs) is critical. How the rapid proliferation of MPs during EM is regulated remains poorly understood. Here, we reveal that ATG7, a
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SinCMat: A single-cell-based method for predicting functional maturation transcription factors Stem. Cell Rep. (IF 5.9) Pub Date : 2024-01-11 Sybille Barvaux, Satoshi Okawa, Antonio del Sol
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Deciphering the differential impact of thrombopoietin/MPL signaling on hematopoietic stem/progenitor cell function in bone marrow and spleen Stem. Cell Rep. (IF 5.9) Pub Date : 2024-01-11 Sandy Lee, Huichun Zhan
Thrombopoietin (TPO) and its receptor MPL play crucial roles in hematopoietic stem cell (HSC) function and platelet production. However, the precise effects of TPO/MPL signaling on HSC regulation in different hematopoietic niches remain unclear. Here, we investigated the effects of TPO/MPL ablation on marrow and splenic hematopoiesis in TPO−/− and MPL−/− mice during aging. Despite severe thrombocytopenia
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Transcription factor co-expression mediates lineage priming for embryonic and extra-embryonic differentiation Stem. Cell Rep. (IF 5.9) Pub Date : 2024-01-11 Alba Redó-Riveiro, Jasmina Al-Mousawi, Madeleine Linneberg-Agerholm, Martin Proks, Marta Perera, Nazmus Salehin, Joshua M. Brickman
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Engineering human pluripotent stem cell lines to evade xenogeneic transplantation barriers Stem. Cell Rep. (IF 5.9) Pub Date : 2024-01-11 Hannah A. Pizzato, Paula Alonso-Guallart, James Woods, Jon P. Connelly, Todd A. Fehniger, John P. Atkinson, Shondra M. Pruett-Miller, Frederick J. Monsma, Deepta Bhattacharya
Successful allogeneic human pluripotent stem cell (hPSC)-derived therapies must overcome immunological rejection by the recipient. To build reagents to define these barriers, we genetically ablated β2M, TAP1, CIITA, CD74, MICA, and MICB to limit expression of HLA-I, HLA-II, and natural killer (NK) cell activating ligands in hPSCs. Transplantation of these cells that also expressed covalent single chain
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Label-free enrichment of human pluripotent stem cell-derived early retinal progenitor cells for cell-based regenerative therapies Stem. Cell Rep. (IF 5.9) Pub Date : 2024-01-04 Yasuaki Iwama, Hiroko Nomaru, Tomohiro Masuda, Yoko Kawamura, Michiru Matsumura, Yuri Murata, Kazuki Teranishi, Kohji Nishida, Sadao Ota, Michiko Mandai, Masayo Takahashi
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A short history of pluripotent stem cells markers Stem. Cell Rep. (IF 5.9) Pub Date : 2023-12-28 Peter W. Andrews, Paul J. Gokhale
The expression of one or more of a small number of molecules, typically cell surface-associated antigens, or transcription factors, is widely used for identifying pluripotent stem cells (PSCs) or for monitoring their differentiation. However, none of these marker molecules are uniquely expressed by PSCs and all are expressed by stem cells that have lost the ability to differentiate. Consequently, none
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C/EBPβ-induced lymphoid-to-myeloid transdifferentiation emulates granulocyte-monocyte progenitor biology Stem. Cell Rep. (IF 5.9) Pub Date : 2023-12-28 Linh Thuy Nguyen, Karin Zimmermann, Elisabeth Kowenz-Leutz, Ramonique Lim, Maria Hofstätter, Alexander Mildner, Achim Leutz
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Gains of 20q11.21 in human pluripotent stem cells: Insights from cancer research Stem. Cell Rep. (IF 5.9) Pub Date : 2023-12-28 Nuša Krivec, Manjusha S. Ghosh, Claudia Spits
The genetic abnormalities observed in hPSC cultures worldwide have been suggested to pose an important hurdle in their safe use in regenerative medicine due to the possibility of oncogenic transformation by mutant cells in the patient posttransplantation. One of the best-characterized genetic lesions in hPSCs is the gain of 20q11.21, found in 20% of hPSC lines worldwide, and strikingly, also amplified
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Generation of canine induced pluripotent stem cells under feeder-free conditions using Sendai virus vector encoding six canine reprogramming factors Stem. Cell Rep. (IF 5.9) Pub Date : 2023-12-21 Masaya Tsukamoto, Kazuto Kimura, Takumi Yoshida, Miyuu Tanaka, Mitsuru Kuwamura, Taro Ayabe, Genki Ishihara, Kei Watanabe, Mika Okada, Minoru Iijima, Mahito Nakanishi, Hidenori Akutsu, Kikuya Sugiura, Shingo Hatoya
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Interspecies chimerism with human embryonic stem cells generates functional human dopamine neurons at low efficiency Stem. Cell Rep. (IF 5.9) Pub Date : 2023-12-21 Hu Wang, Xiling Yin, Jinchong Xu, Li Chen, Senthilkumar S. Karuppagounder, Enquan Xu, Xiaobo Mao, Valina L. Dawson, Ted M. Dawson
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Taking the leap toward human-specific nonanimal methodologies: The need for harmonizing global policies for microphysiological systems Stem. Cell Rep. (IF 5.9) Pub Date : 2023-12-21 Surat Parvatam, David Pamies, Francesca Pistollato, Sonja Beken, Indumathi Mariappan, Adrian Roth, Monica Piergiovanni, Benoît G.C. Maisonneuve, Lorna Ewart, Abhijit Majumder, Prajakta Dandekar, Rahul Date, Kasturi Mahadik, Saravanan Thiyagarajan, Sandra Coecke
With a recent amendment, India joined other countries that have removed the legislative barrier toward the use of human-relevant methods in drug development. Here, global stakeholders weigh in on the urgent need to globally harmonize the guidelines toward the standardization of microphysiological systems. We discuss a possible framework for establishing scientific confidence and regulatory approval
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Subcellular RNA distribution and its change during human embryonic stem cell differentiation Stem. Cell Rep. (IF 5.9) Pub Date : 2023-12-21 Fanqi Zhou, Puwen Tan, Siqi Liu, Le Chang, Jiabin Yang, Mengyao Sun, Yuehong Guo, Yanmin Si, Dong Wang, Jia Yu, Yanni Ma
The spatial localization of RNA within cells is closely related to its function and also involved in cell fate determination. However, the atlas of RNA distribution within cells and dynamic changes during the developmental process are largely unknown. In this study, five subcellular components, including cytoplasmic extract, membrane extract, soluble nuclear extract, chromatin-bound nuclear extract
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Transparency in controversial research: A review of human embryo research publication ethical disclosure statements Stem. Cell Rep. (IF 5.9) Pub Date : 2023-12-21 Akshaya Venkatesh, Ana S. Iltis, Kirstin R.W. Matthews
In 2021, the International Society for Stem Cell Research (ISSCR) released updated guidelines that included human embryo research guidance. Requiring ethics statements in publications using human embryos is one way to verify adherence to these guidelines. A review of top-tier biomedical journal requirements identified only one publisher that requires a human embryo statement. A review of articles using
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The emergence of human gastrulation upon in vitro attachment Stem. Cell Rep. (IF 5.9) Pub Date : 2023-12-14 Riccardo De Santis, Eleni Rice, Gist Croft, Min Yang, Edwin A. Rosado-Olivieri, Ali H. Brivanlou
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Generation of highly pure pluripotent stem cell-derived myogenic progenitor cells and myotubes Stem. Cell Rep. (IF 5.9) Pub Date : 2023-12-14 Reem Bou Akar, Chéryane Lama, Déborah Aubin, Julien Maruotti, Brigitte Onteniente, Joana Esteves de Lima, Frédéric Relaix
Driving efficient and pure skeletal muscle cell differentiation from pluripotent stem cells (PSCs) has been challenging. Here, we report an optimized protocol that generates skeletal muscle progenitor cells with high efficiency and purity in a short period of time. Human induced PSCs (hiPSCs) and murine embryonic stem cells (mESCs) were specified into the mesodermal myogenic fate using distinct and
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Human retinal organoids with an OPA1 mutation are defective in retinal ganglion cell differentiation and function Stem. Cell Rep. (IF 5.9) Pub Date : 2023-12-14 Qiannan Lei, Kangjian Xiang, Lin Cheng, Mengqing Xiang
Autosomal dominant optic atrophy (ADOA), mostly caused by heterozygous OPA1 mutations and characterized by retinal ganglion cell (RGC) loss and optic nerve degeneration, is one of the most common types of inherited optic neuropathies. Previous work using a two-dimensional (2D) differentiation model of induced pluripotent stem cells (iPSCs) has investigated ADOA pathogenesis but failed to agree on the
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Distinct roles of hematopoietic cytokines in the regulation of leukemia stem cells in murine MLL-AF9 leukemia Stem. Cell Rep. (IF 5.9) Pub Date : 2023-12-14 Yanchun Li, Christopher S. Seet, Ryan Mack, Kanak Joshi, Austin P. Runde, Patrick A. Hagen, Kevin Barton, Peter Breslin, Ameet Kini, Hong-Long Ji, Jiwang Zhang
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A critical role of retinoic acid concentration for the induction of a fully human-like atrial action potential phenotype in hiPSC-CM Stem. Cell Rep. (IF 5.9) Pub Date : 2023-12-12 Carl Schulz, Muhammed Sönmez, Julia Krause, Edzard Schwedhelm, Pan Bangfen, Dzenefa Alihodzic, Arne Hansen, Thomas Eschenhagen, Torsten Christ
Abstract not available
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Single-cell transcriptomic analysis of corneal organoids during development Stem. Cell Rep. (IF 5.9) Pub Date : 2023-11-30 Aditi Swarup, Ragini Phansalkar, Maurizio Morri, Aditi Agarwal, Varun Subramaniam, BaoXiang Li, Albert Y. Wu
Corneal organoids are useful tools for disease modeling and tissue transplantation; however, they have not yet been well studied during maturation. We characterized human iPSC-derived corneal organoids at 1, 2, 3, and 4 months of development using single-cell RNA sequencing to determine the cellular heterogeneity at each stage. We found pluripotent cell clusters committed to epithelial cell lineage
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Eyes open on stem cells Stem. Cell Rep. (IF 5.9) Pub Date : 2023-11-30 Anna Altshuler, Aya Amitai-Lange, Waseem Nasser, Shalini Dimri, Swarnabh Bhattacharya, Beatrice Tiosano, Ramez Barbara, Daniel Aberdam, Shigeto Shimmura, Ruby Shalom-Feuerstein
Recently, the murine cornea has reemerged as a robust stem cell (SC) model, allowing individual SC tracing in living animals. The cornea has pioneered seminal discoveries in SC biology and regenerative medicine, from the first corneal transplantation in 1905 to the identification of limbal SCs and their transplantation to successfully restore vision in the early 1990s. Recent experiments have exposed
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ASCL1 induces neurogenesis in human Müller glia Stem. Cell Rep. (IF 5.9) Pub Date : 2023-11-30 Juliette Wohlschlegel, Connor Finkbeiner, Dawn Hoffer, Faith Kierney, Aric Prieve, Alexandria D. Murry, Alexandra K. Haugan, Isabel Ortuño-Lizarán, Fred Rieke, Sam A. Golden, Thomas A. Reh
In mammals, loss of retinal cells due to disease or trauma is an irreversible process that can lead to blindness. Interestingly, regeneration of retinal neurons is a well established process in some non-mammalian vertebrates and is driven by the Müller glia (MG), which are able to re-enter the cell cycle and reprogram into neurogenic progenitors upon retinal injury or disease. Progress has been made
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DOT1L interaction partner AF10 controls patterning of H3K79 methylation and RNA polymerase II to maintain cell identity Stem. Cell Rep. (IF 5.9) Pub Date : 2023-11-22 Coral K. Wille, Edwin N. Neumann, Aniruddha J. Deshpande, Rupa Sridharan
Histone 3 lysine 79 methylation (H3K79me) is enriched on gene bodies proportional to gene expression levels and serves as a strong barrier for the reprogramming of somatic cells to induced pluripotent stem cells (iPSCs). DOT1L is the sole histone methyltransferase that deposits all three orders—mono (me1), di (me2), and tri (me3) methylation—at H3K79. Here, we leverage genetic and chemical approaches
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Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2 Stem. Cell Rep. (IF 5.9) Pub Date : 2023-11-22 Sophie Péron, Leo M. Miyakoshi, Monika S. Brill, Diana Manzano-Franco, Julia Serrano-López, Wenqiang Fan, Nicolás Marichal, Alexander Ghanem, Karl-Klaus Conzelmann, Marisa Karow, Felipe Ortega, Sergio Gascón, Benedikt Berninger
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Deriving early single-rosette brain organoids from human pluripotent stem cells Stem. Cell Rep. (IF 5.9) Pub Date : 2023-11-22 Andrew M. Tidball, Wei Niu, Qianyi Ma, Taylor N. Takla, J. Clayton Walker, Joshua L. Margolis, Sandra P. Mojica-Perez, Roksolana Sudyk, Lu Deng, Shannon J. Moore, Ravi Chopra, Vikram G. Shakkottai, Geoffrey G. Murphy, Yukun Yuan, Lori L. Isom, Jun Z. Li, Jack M. Parent
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Induction of local immunosuppression in allogeneic cell transplantation by cell-type-specific expression of PD-L1 and CTLA4Ig Stem. Cell Rep. (IF 5.9) Pub Date : 2023-11-22 Wenliang Zhu, Mengqi Li, Jun Zou, Da Zhang, Minghui Fang, Yun Sun, Can Li, Mingming Tang, Yukai Wang, Qi Zhou, Tongbiao Zhao, Wei Li, Zheng Hu, Baoyang Hu
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Characterization of human pluripotent stem cell differentiation by single-cell dual-omics analyses Stem. Cell Rep. (IF 5.9) Pub Date : 2023-11-22 Daiyuan Liu, Yuting Fu, Xinru Wang, Xueyi Wang, Xing Fang, Yincong Zhou, Renying Wang, Peijing Zhang, Mengmeng Jiang, Danmei Jia, Jingjing Wang, Haide Chen, Guoji Guo, Xiaoping Han
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Ribosome biogenesis controls cranial suture MSC fate via the complement pathway in mouse and human iPSC models Stem. Cell Rep. (IF 5.9) Pub Date : 2023-11-16 Supawadee Jariyasakulroj, Wei Zhang, Jianhui Bai, Minjie Zhang, Zhipeng Lu, Jian-Fu Chen
Disruption of global ribosome biogenesis selectively affects craniofacial tissues with unclear mechanisms. Craniosynostosis is a congenital craniofacial disorder characterized by premature fusion of cranial suture(s) with loss of suture mesenchymal stem cells (MSCs). Here we focused on ribosomopathy disease gene Snord118, which encodes a small nucleolar RNA (snoRNA), to genetically disturb ribosome
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A novel human iPSC model of COL4A1/A2 small vessel disease unveils a key pathogenic role of matrix metalloproteinases Stem. Cell Rep. (IF 5.9) Pub Date : 2023-11-16 Maha Al-Thani, Mary Goodwin-Trotman, Steven Bell, Krushangi Patel, Lauren K. Fleming, Catheline Vilain, Marc Abramowicz, Stuart M. Allan, Tao Wang, M. Zameel Cader, Karen Horsburgh, Tom Van Agtmael, Sanjay Sinha, Hugh S. Markus, Alessandra Granata
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Does the Act on the Safety of Regenerative Medicine in Japan ensure “safety”?: Implications of low adverse event reporting Stem. Cell Rep. (IF 5.9) Pub Date : 2023-11-16 Tsunakuni Ikka, Taichi Hatta, Yoko Saito, Misao Fujita
Public document analysis reveals that the adverse events reported for therapeutic administration under the Act on the Safety of Regenerative Medicine (ASRM) in Japan are substantially fewer than those under the Pharmaceuticals and Medical Devices Act. This study highlights the flawed reporting mechanisms and unmet legislative intentions of the ASRM.
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Human fetal tissue is critical for biomedical research Stem. Cell Rep. (IF 5.9) Pub Date : 2023-11-16 Justin Brumbaugh, Brian A. Aguado, Tamra Lysaght, Lawrence S.B. Goldstein
Human fetal tissue and cells derived from fetal tissue are crucial for biomedical research. Fetal tissues and cells are used to study both normal development and developmental disorders. They are broadly applied in vaccine development and production. Further, research using cells from fetal tissue is instrumental for studying many infectious diseases, including a broad range of viruses. These widespread
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Integration of xeno-free single-cell cloning in CRISPR-mediated DNA editing of human iPSCs improves homogeneity and methodological efficiency of cellular disease modeling Stem. Cell Rep. (IF 5.9) Pub Date : 2023-11-16 Atefeh Namipashaki, Kealan Pugsley, Xiaodong Liu, Kirra Abrehart, Sue Mei Lim, Guizhi Sun, Marco J. Herold, Jose M. Polo, Mark A. Bellgrove, Ziarih Hawi
The capability to generate induced pluripotent stem cell (iPSC) lines, in tandem with CRISPR-Cas9 DNA editing, offers great promise to understand the underlying genetic mechanisms of human disease. The low efficiency of available methods for homogeneous expansion of singularized CRISPR-transfected iPSCs necessitates the coculture of transfected cells in mixed populations and/or on feeder layers. Consequently
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MSCohi-O lenses for long-term retention of mesenchymal stem cells on ocular surface as a therapeutic approach for chronic ocular graft-versus-host disease Stem. Cell Rep. (IF 5.9) Pub Date : 2023-11-09 Yuanyue Liu, Siqi Song, Youyu Liu, Ting Fu, Yanzheng Guo, Ruoqing Liu, Jiexing Chen, Yanchun Lin, Yaqi Cheng, Yun Li, Tian Guan, Shiqi Ling, Haoyu Zeng
Chronic ocular graft-versus-host disease (oGVHD) is a common complication of allogeneic hematopoietic stem cell transplantation (allo-HSCT) and can lead to vision loss if not diagnosed and treated promptly. Currently, no approved drugs exist for oGVHD treatment. However, umbilical cord-derived mesenchymal stem cells (UCMSCs) have known immunoregulatory properties and have been employed in clinical
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Pharmacologic enhancement of retromer rescues endosomal pathology induced by defects in the Alzheimer’s gene SORL1 Stem. Cell Rep. (IF 5.9) Pub Date : 2023-11-09 Swati Mishra, Allison Knupp, Chizuru Kinoshita, C. Andrew Williams, Shannon E. Rose, Refugio Martinez, Panos Theofilas, Jessica E. Young
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Efficient derivation of transgene-free porcine induced pluripotent stem cells enables in vitro modeling of species-specific developmental timing Stem. Cell Rep. (IF 5.9) Pub Date : 2023-11-09 J. Vanessa Conrad, Susanne Meyer, Pranav S. Ramesh, Jaime A. Neira, Margaret Rusteika, Daniel Mamott, Bret Duffin, Monica Bautista, Jue Zhang, Emily Hiles, Eve M. Higgins, John Steill, Jack Freeman, Zijian Ni, Shiying Liu, Mark Ungrin, Derrick Rancourt, Dennis O. Clegg, Ron Stewart, James A. Thomson, Li-Fang Chu
Sus scrofa domesticus (pig) has served as a superb large mammalian model for biomedical studies because of its comparable physiology and organ size to humans. The derivation of transgene-free porcine induced pluripotent stem cells (PiPSCs) will, therefore, benefit the development of porcine-specific models for regenerative biology and its medical applications. In the past, this effort has been hampered
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Is epigenome editing non-inheritable? Implications for ethics and the regulation of human applications Stem. Cell Rep. (IF 5.9) Pub Date : 2023-11-02 Mitsuru Sasaki-Honda, Kyoko Akatsuka, Tsutomu Sawai
Epigenome editing offers ethical advantages with non-inheritable gene expression control. However, concerns arise regarding potential transgenerational effects in humans. Ethical and regulatory evaluation is crucial, considering recent advancements and enhanced understanding of transgenerational epigenetics in both mammals and humans.
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Identification of glioblastoma stem cell-associated lncRNAs using single-cell RNA sequencing datasets Stem. Cell Rep. (IF 5.9) Pub Date : 2023-11-02 Rasmani Hazra, Raditya Utama, Payal Naik, Alexander Dobin, David L. Spector
Glioblastoma multiforme (GBM) is an aggressive, heterogeneous brain tumor in which glioblastoma stem cells (GSCs) are known culprits of therapy resistance. Long non-coding RNAs (lncRNAs) have been shown to play a critical role in both cancer and normal biology. A few studies have suggested that aberrant expression of lncRNAs is associated with GSCs. However, a comprehensive single-cell analysis of
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Enrichment of FGF8-expressing cells from neurally induced human pluripotent stem cell cultures Stem. Cell Rep. (IF 5.9) Pub Date : 2023-11-02 Nils Offen, Alina Filatova, Ulrike A. Nuber
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A critical role of retinoic acid concentration for the induction of a fully human-like atrial action potential phenotype in hiPSC-CM Stem. Cell Rep. (IF 5.9) Pub Date : 2023-11-02 Carl Schulz, Muhammed Sönmez, Julia Krause, Edzard Schwedhelm, Pan Bangfen, Dzenefa Alihodzic, Arne Hansen, Thomas Eschenhagen, Torsten Christ
Retinoic acid (RA) induces an atrial phenotype in human induced pluripotent stem cells (hiPSCs), but expression of atrium-selective currents such as the ultrarapid (I) and acetylcholine-stimulated K current is variable and less than in the adult human atrium. We suspected methodological issues and systematically investigated the concentration dependency of RA. RA treatment increased I concentration
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AT7867 promotes pancreatic progenitor differentiation of human iPSCs Stem. Cell Rep. (IF 5.9) Pub Date : 2023-11-02 Nerea Cuesta-Gomez, Kevin Verhoeff, Nidheesh Dadheech, Rena Pawlick, Braulio Marfil-Garza, Haide Razavy, A.M. James Shapiro
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Mowat-Wilson syndrome factor ZEB2 controls early formation of human neural crest through BMP signaling modulation Stem. Cell Rep. (IF 5.9) Pub Date : 2023-10-26 Rebekah M. Charney, Maneeshi S. Prasad, Czarina Juan-Sing, Lipsa J. Patel, Jacqueline C. Hernandez, Jie Wu, Martín I. García-Castro
Mowat-Wilson syndrome is caused by mutations in , with patients exhibiting characteristics indicative of neural crest (NC) defects. We examined the contribution of ZEB2 to human NC formation using a model based on human embryonic stem cells. We found to be one of the earliest factors expressed in prospective human NC, and knockdown revealed a role for ZEB2 in establishing the NC state while repressing
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Businesses marketing purported stem cell treatments and exosome therapies for COVID-19: An analysis of direct-to-consumer online advertising claims Stem. Cell Rep. (IF 5.9) Pub Date : 2023-10-26 Leigh Turner, Juan Ramon Martinez Jr., Shemms Najjar, Thevin Rajapaksha Arachchilage, Jia Chieng Wang
We identified 38 businesses advertising purported stem cell interventions and exosome products for COVID-19. These companies operated or facilitated access to 60 clinics. More than 75% of these clinics were based in the United States and Mexico. Thirty-six of the businesses marketed their stem cell and exosome products as treatments for Long COVID, six advertised them as “immune boosters,” five claimed
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Reconstituted ovaries self-assemble without an ovarian surface epithelium Stem. Cell Rep. (IF 5.9) Pub Date : 2023-10-26 Enrique Sosa, Sinthia Kabir Mumu, Christian C. Alvarado, Qiu Ya Wu, Isaias Roberson, Alejandro Espinoza, Fei-man Hsu, Kaori Saito, Timothy J. Hunt, Jared E. Faith, Matthew G. Lowe, Jonathan A. DiRusso, Amander T. Clark
Three-dimensional (3D) stem cell models of the ovary have the potential to benefit women’s reproductive health research. One such model, the reconstituted ovary (rOvary) self-assembles with pluripotent stem cell-derived germ cells creating a 3D ovarian mimic competent to support the differentiation of functional oocytes inside follicles. In this study, we evaluated the cellular composition of the rOvary
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The multi-lineage transcription factor ISL1 controls cardiomyocyte cell fate through interaction with NKX2.5 Stem. Cell Rep. (IF 5.9) Pub Date : 2023-10-19 Bonnie E.J. Maven, Casey A. Gifford, Melanie Weilert, Barbara Gonzalez-Teran, Ruth Hüttenhain, Angelo Pelonero, Kathryn N. Ivey, Kaitlen Samse-Knapp, Wesley Kwong, David Gordon, Michael McGregor, Tomohiro Nishino, Eyuche Okorie, Sage Rossman, Mauro W. Costa, Nevan J. Krogan, Julia Zeitlinger, Deepak Srivastava
Congenital heart disease often arises from perturbations of transcription factors (TFs) that guide cardiac development. ISLET1 (ISL1) is a TF that influences early cardiac cell fate, as well as differentiation of other cell types including motor neuron progenitors (MNPs) and pancreatic islet cells. While lineage specificity of ISL1 function is likely achieved through combinatorial interactions, its