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Mesenchymal stem cells as a double-edged sword in tumor growth: focusing on MSC-derived cytokines Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2021-01-20 Wenqing Liang; Xiaozhen Chen; Songou Zhang; Jian Fang; Meikai Chen; Yifan Xu; Xuerong Chen
Mesenchymal stem cells (MSCs) show homing capacity towards tumor sites. Numerous reports indicate that they are involved in multiple tumor-promoting processes through several mechanisms, including immunosuppression; stimulation of angiogenesis; transition to cancer-associated fibroblasts; inhibition of cancer cell apoptosis; induction of epithelial–mesenchymal transition (EMT); and increase metastasis
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Iron overload adversely effects bone marrow haematogenesis via SIRT-SOD2-mROS in a process ameliorated by curcumin Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2021-01-13 Shujuan Zhou; Lan Sun; Shanhu Qian; Yongyong Ma; Ruye Ma; Yuqing Dong; Yifen Shi; Songfu Jiang; Haige Ye; Zhijian Shen; Shenghui Zhang; Jianping Shen; Kang Yu; Siqian Wang
Iron overload, which is common in patients with haematological disorders, is known to have a suppressive effect on haematogenesis. However, the mechanism for this effect is still unclear. The antioxidant curcumin has been reported to protect against iron overload-induced bone marrow damage through an as-yet-unknown mechanism. We established iron overload cell and mouse models. Mitochondrial reactive
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Beyond K48 and K63: non-canonical protein ubiquitination Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2021-01-05 Michal Tracz; Wojciech Bialek
Protein ubiquitination has become one of the most extensively studied post-translational modifications. Originally discovered as a critical element in highly regulated proteolysis, ubiquitination is now regarded as essential for many other cellular processes. This results from the unique features of ubiquitin (Ub) and its ability to form various homo- and heterotypic linkage types involving one of
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In vitro differentiation of W8B2+ human cardiac stem cells: gene expression of ionic channels and spontaneous calcium activity Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-11-05 Oualid Ayad; Zeina R. Al Sayed; Stéphane Sebille; Christophe Magaud; Charles-Albert Chapotte-Baldacci; Christophe Jayle; Jean-François Faivre; Nathalie Gaborit; Aurélien Chatelier; Patrick Bois
Human cardiac stem cells expressing the W8B2 marker (W8B2+ CSCs) were recently identified and proposed as a new model of multipotent CSCs capable of differentiating into smooth muscle cells, endothelial cells and immature myocytes. Nevertheless, no characterization of ion channel or calcium activity during the differentiation of these stem cells has been reported. The objectives of this study were
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MicroRNA-296-5p inhibits cell metastasis and invasion in nasopharyngeal carcinoma by reversing transforming growth factor-β-induced epithelial–mesenchymal transition Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-11-03 Meihui Chen; Chen Chen; Haiqing Luo; Jing Ren; Qiuqin Dai; Wenjia Hu; Keyuan Zhou; Xudong Tang; Xiangyong Li
To explore the effect of miR-296-5p on the metastasis of nasopharyngeal carcinoma (NPC) cells and investigate the underlying mechanism. The expressions of miR-296-5p in NPC tissues and cells were determined using GSE32920 database analysis and real-time PCR and miRNA microarray assays. An miR-296-5p mimic and inhibitor were transfected into NPC cells. Then, immunofluorescence imaging, scratch wound-healing
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The nuclear receptor 4A family members: mediators in human disease and autophagy Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-11-03 Liqun Chen; Fengtian Fan; Lingjuan Wu; Yiyi Zhao
The Nuclear receptor 4A (NR4A) subfamily, which belongs to the nuclear receptor (NR) superfamily, has three members: NR4A1 (Nur77), NR4A2 (Nurr1) and NR4A3 (Nor1). They are gene regulators with broad involvement in various signaling pathways and human disease responses, including autophagy. Here, we provide a concise overview of the current understanding of the role of the NR4A subfamily members in
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hsa_circ_0006916 promotes hepatocellular carcinoma progression by activating the miR-337-3p/STAT3 axis Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-11-02 Xiao-Yong Zhou; Hui Yang; Yang-Qiu Bai; Xiu-Ling Li; Shuang-Yin Han; Bing-Xi Zhou
Circular RNAs (circRNAs) are thought to be involved in the development of various malignancies. The expression and function of hsa_circ_0006916, a newly identified circRNA, in hepatocellular carcinoma remain unclear. Quantitative RT-PCR was used to detect hsa_circ_0006916 in hepatocellular carcinoma. In vitro function assays were conducted to explore growth and invasion of hepatocellular carcinoma
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Obituary: in memory of Professor Tadeusz Chojnacki (1932–2020) Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-10-20 Ewa Swiezewska; Anna Chojnacka
Professor Tadeusz Chojnacki passed away on June 13, 2020. Professor Chojnacki was a full member of the Polish Academy of Sciences (PAS), a co-founder and vice-director of the Institute of Biochemistry and Biophysics (IBB) of the Polish Academy of Sciences, and a member of the Scientific Council of IBB PAS and the Scientific Councils of a number of scientific institutions. Professor Chojnacki was also
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Upregulation of miR-361-3p suppresses serotonin-induced proliferation in human pulmonary artery smooth muscle cells by targeting SERT Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-10-07 Ying Zhang; Yongbin Chen; Guo Chen; Yingling Zhou; Hua Yao; Hong Tan
Abnormal proliferation of pulmonary artery smooth muscle cells (PASMCs) is a key mechanism in pulmonary arterial hypertension (PAH). Serotonin (5-hydroxytryptamine, 5-HT) can induce abnormal proliferation of PASMCs. The role of miR-361-3p in serotonin-induced abnormal PASMCs proliferation remains unclear. The miR-361-3p level was analyzed in plasma from PAH patients and normal controls and in human
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Arsenic trioxide as a novel anti-glioma drug: a review Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-09-24 Yi Fang; Zhen Zhang
Arsenic trioxide has shown a strong anti-tumor effect with little toxicity when used in the treatment of acute promyelocytic leukemia (APL). An effect on glioma has also been shown. Its mechanisms include regulation of apoptosis and autophagy; promotion of the intracellular production of reactive oxygen species, causing oxidative damage; and inhibition of tumor stem cells. However, glioma cells and
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Knockdown of LINC00665 inhibits proliferation and invasion of breast cancer via competitive binding of miR-3619-5p and inhibition of catenin beta 1 Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-09-24 Minhao Lv; Qixin Mao; Juntao Li; Jianghua Qiao; Xiuchun Chen; Suxia Luo
Long intergenic non-protein coding RNA00665 (LINC00665) plays a crucial tumorigenic role in many cancers, such as gastric cancer and lung adenocarcinoma. However, its role and mechanism of action in the progression of breast cancer (BC) are unknown. LINC00665 expression levels were determined using quantitative polymerase chain reaction analysis with BC tissues and cell lines. BC cell proliferation
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Comparative transcriptome analysis of the main beam and brow tine of sika deer antler provides insights into the molecular control of rapid antler growth. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-09-07 Baojin Yao,Chaonan Wang,Zhenwei Zhou,Mei Zhang,Daqing Zhao,Xueyuan Bai,Xiangyang Leng
Deer antlers have become a valuable model for biomedical research due to the capacities of regeneration and rapid growth. However, the molecular mechanism of rapid antler growth remains to be elucidated. The aim of the present study was to compare and explore the molecular control exerted by the main beam and brow tine during rapid antler growth. The main beams and brow tines of sika deer antlers were
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The MDM2 ligand Nutlin-3 differentially alters expression of the immune blockade receptors PD-L1 and CD276. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-08-31 Ruidong Li,Pavlina Zatloukalova,Petr Muller,Maria Gil-Mir,Sachin Kote,Simon Wilkinson,Alain J Kemp,Lenka Hernychova,Yaxin Wang,Kathryn L Ball,Kaixiong Tao,Ted Hupp,Borivoj Vojtesek
The links between the p53/MDM2 pathway and the expression of pro-oncogenic immune inhibitory receptors in tumor cells are undefined. In this report, we evaluate whether there is p53 and/or MDM2 dependence in the expression of two key immune receptors, CD276 and PD-L1. Proximity ligation assays were used to quantify protein-protein interactions in situ in response to Nutlin-3. A panel of p53-null melanoma
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Forced expression of miR-143 and -145 in cardiomyocytes induces cardiomyopathy with a reductive redox shift. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-08-24 Kota Ogawa,Akiko Noda,Jun Ueda,Takehiro Ogata,Rumiko Matsuyama,Yuji Nishizawa,Shanlou Qiao,Satoru Iwata,Morihiro Ito,Yoshitaka Fujihara,Masatoshi Ichihara,Koichi Adachi,Yuji Takaoka,Takashi Iwamoto
Animal model studies show that reductive stress is involved in cardiomyopathy and myopathy, but the exact physiological relevance remains unknown. In addition, the microRNAs miR-143 and miR-145 have been shown to be upregulated in cardiac diseases, but the underlying mechanisms associated with these regulators have yet to be explored. We developed transgenic mouse lines expressing exogenous miR-143
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Specific functions of Exostosin-like 3 (EXTL3) gene products. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-08-20 Shuhei Yamada
Exostosin-like 3 (EXTL3) encodes the glycosyltransferases responsible for the biosynthesis of the backbone structure of heparan sulfate (HS), a sulfated polysaccharide that is ubiquitously distributed on the animal cell surface and in the extracellular matrix. A lack of EXTL3 reduces HS levels and causes embryonic lethality, indicating its indispensable role in the biosynthesis of HS. EXTL3 has also
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Effects of HPV16 E6 protein on Daxx-induced apoptosis in C33A cells. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-08-08 Shuangyang Tang,Shuang Ding,Lan Yu,Haiyan Shen,Yanping Wan,Yimou Wu
Daxx is a highly conserved nuclear protein with an important role in transcription, apoptosis and other cell processes. We investigated the role of HPV16 E6 in Daxx-induced apoptosis through their interactions in C33A cells. The binding of HPV16 E6 and Daxx was confirmed in C33A cells using co-immunoprecipitation and indirect immunofluorescence assays. Quantitative PCR and western blotting were performed
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Do ABC transporters regulate plasma membrane organization? Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-07-06 Ambroise Wu,Karolina Wojtowicz,Stephane Savary,Yannick Hamon,Tomasz Trombik
The plasma membrane (PM) spatiotemporal organization is one of the major factors controlling cell signaling and whole-cell homeostasis. The PM lipids, including cholesterol, determine the physicochemical properties of the membrane bilayer and thus play a crucial role in all membrane-dependent cellular processes. It is known that lipid content and distribution in the PM are not random, and their transversal
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Silibinin suppresses epithelial-mesenchymal transition in human non-small cell lung cancer cells by restraining RHBDD1. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-06-09 Suyan Xu,Hongyan Zhang,Aifeng Wang,Yongcheng Ma,Yuan Gan,Guofeng Li
Rhomboid domain containing 1 (RHBDD1) plays a crucial role in tumorigenesis. Silibinin, which is a natural extract from milk thistle, has shown anti-tumor effects against various tumors. Here, we investigate whether silibinin affects the function of RHBDD1 in non-small cell lung cancer (NSCLC) cell proliferation, migration and invasion. The Oncomine database and an immunohistochemistry (IHC) assay
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The usefulness of lactate dehydrogenase measurements in current oncological practice. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-06-09 Agata Forkasiewicz,Maja Dorociak,Kamilla Stach,Piotr Szelachowski,Renata Tabola,Katarzyna Augoff
One of the hallmarks of cancer cells is increased energy requirements associated with the higher rate of cellular proliferative activity. Metabolic changes in rapidly dividing cancer cells are closely associated with increased uptake of glucose and abnormal activity of lactate dehydrogenase (LDH), which regulates the processing of glucose to lactic acid. As serum LDH levels were found to be commonly
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LncRNA NNT-AS1 promotes lung squamous cell carcinoma progression by regulating the miR-22/FOXM1 axis. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-05-29 Jing Ma,Guanbin Qi,Lei Li
Recent studies have revealed that dysregulated expression of long non-coding RNA nicotinamide nucleotide transhydrogenase antisense RNA 1 (lncRNA NNT-AS1) is associated with cell tumorigenicity in non-small cell lung cancer. However, the exact molecular mechanisms of NNT-AS1 in lung squamous cell carcinoma (LUSC) remain largely unknown. The expression of NNT-AS1, microRNA (miR)-22 and Forkhead box
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Asiaticoside inhibits TGF-β1-induced mesothelial-mesenchymal transition and oxidative stress via the Nrf2/HO-1 signaling pathway in the human peritoneal mesothelial cell line HMrSV5. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-05-29 Junyi Zhao,Jun Shi,Yun Shan,Manshu Yu,Xiaolin Zhu,Yilin Zhu,Li Liu,Meixiao Sheng
Peritoneal fibrosis (PF) is a frequent complication caused by peritoneal dialysis (PD). Peritoneal mesothelial cells (PMCs), the first barrier of the peritoneum, play an important role in maintaining structure and function in the peritoneum during PD. Mesothelial-mesenchymal transition (MMT) and oxidative stress of PMCs are two key processes of PF. To elucidate the efficacy and possible mechanism of
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On the physiological and cellular homeostasis of ascorbate. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-05-29 Magdalena Przybyło,Marek Langner
Recent interest in the role of ascorbate in crucial metabolic processes is driven by the growing number of medical reports that show beneficial effects of ascorbate supplementation for maintaining general well-being and recovery from a variety of medical conditions. The effect of ascorbate on the local body environment highly depends on its local concentration; at low concentrations it can cause the
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Role of angiotensin-converting enzyme in myeloid cell immune responses. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-05-25 Duo-Yao Cao,Suguru Saito,Luciana C Veiras,Derick Okwan-Duodu,Ellen A Bernstein,Jorge F Giani,Kenneth E Bernstein,Zakir Khan
Angiotensin-converting enzyme (ACE), a dicarboxypeptidase, plays a major role in the regulation of blood pressure by cleaving angiotensin I into angiotensin II (Ang II), a potent vasoconstrictor. Because of its wide substrate specificity and tissue distribution, ACE affects many diverse biological processes. In inflammatory diseases, including granuloma, atherosclerosis, chronic kidney disease and
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Downregulation of miR-637 promotes vascular smooth muscle cell proliferation and migration via regulation of insulin-like growth factor-2. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-05-07 Ning Yang,Bo Dong,Yanqiu Song,Yang Li,Lu Kou,Jingyu Yang,Qin Qin
Dysregulation of the proliferation and migration of vascular smooth muscle cells (VSMCs) is a crucial cause of atherosclerosis. MiR-637 exerts an antiproliferative effect on multiple human cells. Its impact on atherosclerosis remains largely unexplored. Real-time PCR was used to determine miR-637 expression in samples from atherosclerosis patients and animal models. Its expression in VSMC dysfunction
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LncRNA DRAIC inhibits proliferation and metastasis of gastric cancer cells through interfering with NFRKB deubiquitination mediated by UCHL5. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-04-25 Zheng Zhang,Xiaoxuan Hu,Jia Kuang,Jinmao Liao,Qi Yuan
Long non-coding RNA (lncRNA) as a widespread and pivotal epigenetic molecule participates in the occurrence and progression of malignant tumors. DRAIC, a kind of lncRNA whose coding gene location is on 15q23 chromatin, has been found to be weakly expressed in a variety of malignant tumors and acts as a suppressor, but its characteristics and role in gastric cancer (GC) remain to be elucidated. Sixty-seven
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Targeting the 4-1BB costimulatory molecule through single chain antibodies promotes the human T-cell response. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-04-22 Salman Bagheri,Elmira Safaie Qamsari,Mehdi Yousefi,Farhad Riazi-Rad,Zahra Sharifzadeh
Adoptive T-cell therapy (ACT) using autologous tumor-reactive T lymphocytes has considerable potential for cancer immunotherapy. In ACT, T cells are isolated from cancer patients and then stimulated and expanded in vitro by cytokines and costimulatory molecules. 4-1BB is an important costimulatory protein belonging to the TNF receptor superfamily. It is involved in T-cell survival, proliferation and
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MicroRNA-186-5p serves as a diagnostic biomarker in atherosclerosis and regulates vascular smooth muscle cell proliferation and migration. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-04-21 Bin Sun,Qingtao Cao,Meng Meng,Xiaolong Wang
MicroRNA dysregulation occurs in many human diseases, including atherosclerosis. Here, we examined the serum expression and clinical significance of miR-186-5p in patients with atherosclerosis, and explored its influence on vascular smooth muscle cell (VSMC) proliferation and migration. Blood samples were collected from 104 patients with asymptomatic atherosclerosis and 80 healthy controls. Quantitative
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The role of miR-711 in cardiac cells in response to oxidative stress and its biogenesis: a study on H9C2 cells. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-04-09 Duo Zhao,Hao Zheng,Adam Greasley,Fengjun Ling,Qinfeng Zhou,Bowen Wang,Tiffany Ni,Ishita Topiwala,Cuilin Zhu,Tina Mele,Kexiang Liu,Xiufen Zheng
Oxidative stress results in cell apoptosis/death and plays a detrimental role in disease development and progression. Stressors alter the miRNA expression profile and miRNAs play a role in the cell response to stress. We previously showed that miR-711 is significantly over-expressed in extended cold ischemia reperfusion injured hearts in heart transplant. In this study, we aimed to investigate the
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Quantitative proteomics analysis of differentially expressed proteins induced by astragaloside IV in cervical cancer cell invasion. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-03-31 Chenglai Xia,Zhihong He,Yantao Cai
Cervical cancer remains the second leading cause of mortality in women in developing countries. While surgery, chemotherapy, radiotherapy, and vaccine therapy are being applied for its treatment, individually or in combination, the survival rate in advanced cervical cancer patients is still very low. Traditional Chinese medicine has been found to be effective in the treatment of cervical cancer. Astragaloside
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TCF 4 tumor suppressor: a molecular target in the prognosis of sporadic colorectal cancer in humans. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-03-31 Mumtaz Anwar,Pooja Malhotra,Rakesh Kochhar,Alka Bhatia,Akhtar Mahmood,Rajinder Singh,Safrun Mahmood
A huge array of function is played by the Wnt/β-catenin signaling pathway in development by balancing gene expression through the modulation of cell-specific DNA binding downstream effectors such as T-cell factor/lymphoid enhancer factor (TCF/LEF). The β-catenin/TCF-4 complex is a central regulatory switch for differentiation and proliferation of intestinal cells (both normal and malignant). Thus,
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The role of phosphatidylserine recognition receptors in multiple biological functions Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-03-26 Mehri Bemani Naeini; Vanessa Bianconi; Matteo Pirro; Amirhossein Sahebkar
Apoptotic cells are rapidly engulfed and degraded by phagocytes through efferocytosis. Efferocytosis is a highly regulated process. It is triggered upon the activation of caspase-dependent apoptosis, which in turn promotes the expression of “eat me” signals on the surface of dying cells and the release of soluble “find me” signals for the recruitment of phagocytes. To date, many “eat me” signals have
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MiR-206 may suppress non-small lung cancer metastasis by targeting CORO1C. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-03-17 Ming Liao,Lijun Peng
Non-small lung cancer (NSCLC), with a poor 5-year survival rate (16%), is the major type of lung cancer. Metastasis has been identified as the main factor that leads to NSCLC therapy failure. MiR-206 is a metastasis suppressor in many cancers, including colorectal cancer, renal cell carcinoma and breast cancer. However, the role of miR-206 in NSCLC metastasis and the underlying mechanism are still
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LncRNA CASC2 inhibits hypoxia-induced pulmonary artery smooth muscle cell proliferation and migration by regulating the miR-222/ING5 axis. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-03-17 Yan Han,Yuhao Liu,Chaokuan Yang,Chuanyu Gao,Xiaoyan Guo,Jiangtao Cheng
Pulmonary arterial hypertension (PAH) is often characterized by cell proliferation and migration of pulmonary arterial smooth muscle cells (PASMCs). LncRNA cancer susceptibility candidate 2 (CASC2) has been revealed to be involved in PASMC injury in hypoxia-induced pulmonary hypertension. However, the exact molecular mechanisms whereby CASC2 regulates PASMC proliferation and migration are still incompletely
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Downregulation of miR-483-5p decreases hypoxia-induced injury in human cardiomyocytes by targeting MAPK3. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-03-17 Yan Hao,Haitao Yuan,Houzhi Yu
MiR-483-5p was recently identified as a risk factor in the early stages of acute myocardial infarction (AMI) patients. Here, we further investigated how miR-483-5p affects cardiomyocyte apoptosis and oxidative stress under hypoxic conditions. Plasma samples were collected from AMI patients and healthy volunteers. The expression of miR-483-5p was determined using quantitative real-time PCR. An in vitro
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miR-144-3p inhibits cell proliferation of colorectal cancer cells by targeting BCL6 via inhibition of Wnt/β-catenin signaling. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-03-17 Naihui Sun,Liang Zhang,Chongguang Zhang,Yuan Yuan
It has been shown that miR-144-3p regulates cell proliferation, apoptosis, migration and invasion in various cancers. However, the function and expression of miR-144-3p in colorectal cancer (CRC) remained obscure. Immunohistochemical (IHC) staining was performed to investigate the protein expression of BCL6 in CRC tissues. The effect of BCL6 and miR-144-3p on CRC cells was explored through methylthiazolyl
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Unfolded protein response (UPR) integrated signaling networks determine cell fate during hypoxia Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-03-13 Sylwia Bartoszewska; James F. Collawn
During hypoxic conditions, cells undergo critical adaptive responses that include the up-regulation of hypoxia-inducible proteins (HIFs) and the induction of the unfolded protein response (UPR). While their induced signaling pathways have many distinct targets, there are some important connections as well. Despite the extensive studies on both of these signaling pathways, the exact mechanisms involved
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Blockage of O-linked GlcNAcylation induces AMPK-dependent autophagy in bladder cancer cells Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-03-10 Lu Jin; Feng Yuan; Guangcheng Dai; Qiu Yao; Han Xiang; Lixia Wang; Boxin Xue; Yuxi Shan; Xiaolong Liu
High levels of the post-translational modification O-GlcNAcylation (O-GlcNAc) are found in multiple cancers, including bladder cancer. Autophagy, which can be induced by stress from post-translational modifications, plays a critical role in maintaining cellular homeostasis and regulating tumorigenesis. The impact of O-GlcNAcylation on autophagy in bladder cancer remains unclear. Here, we evaluate the
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Adipose-derived stromal cells in regulation of hematopoiesis Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-03-05 Jing Zhang; Yunsheng Liu; Wen Yin; Xingbin Hu
Over the past decade, mesenchymal stromal cells (MSCs) found in the bone marrow microenvironment have been considered to be important candidates in cellular therapy. However, the application of MSCs in clinical settings is limited by the difficulty and low efficiency associated with the separation of MSCs from the bone marrow. Therefore, distinct sources of MSCs have been extensively explored. Adipose-derived
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Metabolic host response and therapeutic approaches to influenza infection Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-03-05 Mohsen Keshavarz; Farid Solaymani-Mohammadi; Haideh Namdari; Yaser Arjeini; Mohammad Javad Mousavi; Farhad Rezaei
Based on available metabolomic studies, influenza infection affects a variety of cellular metabolic pathways to ensure an optimal environment for its replication and production of viral particles. Following infection, glucose uptake and aerobic glycolysis increase in infected cells continually, which results in higher glucose consumption. The pentose phosphate shunt, as another glucose-consuming pathway
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MicroRNA-125b exerts antitumor functions in cutaneous squamous cell carcinoma by targeting the STAT3 pathway Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-03-05 Ke Tian; Wanggen Liu; Jing Zhang; Xiaoyi Fan; Jingyuan Liu; Nan Zhao; Chunxia Yao; Guoying Miao
MicroRNA-125b (miR-125b) is downregulated in human cutaneous squamous cell carcinoma (CSCC). However, its function in CSCC has yet to be extensively explored. Here, we analyze the relationship between signal transducer and activator of transcription 3 (STAT3) and miR-125b in CSCC. Western blotting and quantitative RT-PCR were used to determine the expression of the miR-125b–STAT3 axis in human CSCC
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Ferrostatin-1 alleviates lipopolysaccharide-induced acute lung injury via inhibiting ferroptosis Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-02-27 Pengfei Liu; Yetong Feng; Hanwei Li; Xin Chen; Guangsuo Wang; Shiyuan Xu; Yalan Li; Lei Zhao
Ferroptosis is a newly recognized type of cell death, which is different from traditional necrosis, apoptosis or autophagic cell death. However, the position of ferroptosis in lipopolysaccharide (LPS)-induced acute lung injury (ALI) has not been explored intensively so far. In this study, we mainly analyzed the relationship between ferroptosis and LPS-induced ALI. In this study, a human bronchial epithelial
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GNAS promotes inflammation-related hepatocellular carcinoma progression by promoting STAT3 activation. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-02-24 Hongda Ding,Xixia Zhang,Yang Su,Changjun Jia,Chaoliu Dai
Hepatocellular carcinoma (HCC) is still the most common cause of cancer-related mortality worldwide and accumulating studies report that HCC is frequently linked to chronic inflammation. G-protein alpha-subunit (GNAS)-activating mutations have recently been reported to form a rare subgroup of inflammatory liver tumors. In this study, we investigated the roles of GNAS in inflammation-related HCC progression
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Inhibition of microRNA-15 protects H9c2 cells against CVB3-induced myocardial injury by targeting NLRX1 to regulate the NLRP3 inflammasome. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-02-19 Ru Tong,Tiewen Jia,Ruijie Shi,Futang Yan
Viral myocarditis (VMC) is a type of cardiac inflammation that is generally caused by coxsackievirus B3 (CVB3) infection. Several MicroRNAs (miRNAs) are known to play crucial roles in VMC pathogenesis. MiR-15 is reportedly associated with myocardial injury, inflammatory responses and viral infection. Whether miR-15 affects the occurrence and development of VMC remains largely unknown. The roles of
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MicroRNA-429 inhibits neuroblastoma cell proliferation, migration and invasion via the NF-κB pathway. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-02-13 Xianjun Zhou,Hongting Lu,Fujiang Li,Xiwei Hao,Lulu Han,Qian Dong,Xin Chen
MicroRNAs (miRNAs or miRs) can participate in the development and progression of neuroblastoma. Many studies have indicated that miR-429 can participate in tumor development. However, the mechanism underlying miR-429-mediated progression of neuroblastoma remains largely unclear. Colony formation and apoptosis assays were used to determine the effect of miR-429 on cell proliferation. Its impact on cell
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LINC00511 promotes proliferation and invasion by sponging miR-515-5p in gastric cancer. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-02-03 Di Wang,Ketong Liu,Erdong Chen
Long non-coding RNAs (lncRNAs) are known to be involved in tumorigenesis. The functions of LINC00511 in gastric cancer are poorly understood. Quantitative RT-PCR was performed to investigate the levels of LINC00511 in gastric cancer tissues and cell lines. CCK-8, flow cytometry, wound-healing and Transwell assays were performed to examine cellular functions. The underlying regulatory mechanisms of
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Deficiency of αII-spectrin affects endothelial cell-matrix contact and migration leading to impairment of angiogenesis in vitro. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-02-03 Beata Machnicka,Aurélie Ponceau,Julien Picot,Yves Colin,Marie-Christine Lecomte
Precise coordination of cytoskeletal components and dynamic control of cell adhesion and migration are required for crucial cell processes such as differentiation and morphogenesis. We investigated the potential involvement of αII-spectrin, a ubiquitous scaffolding element of the membrane skeleton, in the adhesion and angiogenesis mechanism. The cell models were primary human umbilical vein endothelial
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DNA-dependent protein kinase in telomere maintenance and protection. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-01-17 Jiangdong Sui,Shichuan Zhang,Benjamin P C Chen
This review focuses on DNA–dependent protein kinase (DNA–PK), which is the key regulator of canonical non–homologous end–joining (NHEJ), the predominant mechanism of DNA double–strand break (DSB) repair in mammals. DNA–PK consists of the DNA–binding Ku70/80 heterodimer and the catalytic subunit DNA–PKcs. They assemble at DNA ends, forming the active DNA–PK complex, which initiates NHEJ–mediated DSB
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The ubiquitin system: orchestrating cellular signals in non-small-cell lung cancer. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2020-01-16 Qiang Fan,Qian Wang,Renjie Cai,Haihua Yuan,Ming Xu
The ubiquitin system, known as a common feature in eukaryotes, participates in multiple cellular processes, such as signal transduction, cell-cycle progression, receptor trafficking and endocytosis, and even the immune response. In lung cancer, evidence has revealed that aberrant events in ubiquitin-mediated processes can cause a variety of pathological outcomes including tumorigenesis and metastasis
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The novel circular RNA circ-CAMK2A enhances lung adenocarcinoma metastasis by regulating the miR-615-5p/fibronectin 1 pathway Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2019-12-26 Jiahui Du; Guangzhao Zhang; Hongli Qiu; Haifeng Yu; Wuying Yuan
Circular RNA (circRNA) has recently been considered as a key regulator in carcinogenesis. In this study, we investigated the functional significance and regulatory role of circ-CAMK2A (hsa_circ_0128332) in lung adenocarcinoma (LUAD). GSE101586 was employed to screen differentially expressed circRNAs. = Relative expression levels of circ-CAMK2A, miR-615-5p, fibronectin 1 (FN1), MMP2, and MMP9 were tested
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SOX30, a target gene of miR-653-5p, represses the proliferation and invasion of prostate cancer cells through inhibition of Wnt/β-catenin signaling Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2019-12-23 Qiang Fu; Zhenye Sun; Fan Yang; Tianci Mao; Yanyao Gao; He Wang
Sex-determining region Y-box containing gene 30 (SOX30) is a newly identified tumor-associated gene in several types of cancer. However, whether SOX30 is involved in the development and progression of prostate cancer remains unknown. This study investigated the potential role of SOX30 in prostate cancer. Prostate cancer cell lines and a normal prostate epithelial cell line were used for the experiments
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The long noncoding RNA LINC00483 promotes lung adenocarcinoma progression by sponging miR-204-3p Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2019-12-16 Shengzhuang Yang; Tao Liu; Yu Sun; Xiangsen Liang
The expression of the long noncoding RNA LINC00483 is upregulated in lung adenocarcinoma (LUAD). However, its role in the progression of LUAD and the underlying mechanisms remain elusive. The expressions of LINC00483 and miR-204-3p were determined using quantitative real-time PCR. The correlation between the clinicopathological characteristics of LUAD patients and LINC00483 expression was analyzed
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Editorial focus: understanding off-target effects as the key to successful RNAi therapy Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2019-12-09 Rafal Bartoszewski; Aleksander F. Sikorski
With the first RNA interference (RNAi) drug (ONPATTRO (patisiran)) on the market, we witness the RNAi therapy field reaching a critical turning point, when further improvements in drug candidate design and delivery pipelines should enable fast delivery of novel life changing treatments to patients. Nevertheless, ignoring parallel development of RNAi dedicated in vitro pharmacological profiling aiming
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MiR-214 prevents the progression of diffuse large B-cell lymphoma by targeting PD-L1. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2019-12-04 Jing-Ran Sun,Xiao Zhang,Ya Zhang
We explored the role and mechanism of miR-214 involvement in the progression of diffuse large B-cell lymphoma (DLBCL). The expression levels of miR-214 and PD-L1 in human DLBCL cell lines and in tissue samples from patients with DLBCL were determined using quantitative RT-PCR. The dual-luciferase reporter assay was employed to determine the correlation between the expressions of miR-214 and PD-L1.
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Upregulation of miR-376c-3p alleviates oxygen-glucose deprivation-induced cell injury by targeting ING5. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2019-12-04 Heng Zhang,Jie Zhou,Mingxia Zhang,Yanjie Yi,Bing He
The expression level of miR-376c-3p is significantly lower in infants with neonatal hypoxic-ischemic encephalopathy (HIE) than in healthy infants. However, the biological function of this microRNA remains largely elusive. We used PC-12 and SH-SY5Y cells to establish an oxygen–glucose deprivation (OGD) cell injury model to mimic HIE in vitro. The miR-376c-3p expression levels were measured using quantitative
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MicroRNA-582-3p negatively regulates cell proliferation and cell cycle progression in acute myeloid leukemia by targeting cyclin B2. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2019-12-04 Haixia Li,Xuefei Tian,Paoqiu Wang,Mao Huang,Ronghua Xu,Tian Nie
MicroRNAs (miRNAs) function as post-transcriptional gene expression regulators. Some miRNAs, including the recently discovered miR-582–3p, have been implicated in leukemogenesis. This study aimed to reveal the biological function of miR-582–3p in acute myeloid leukemia (AML), which is one of the most frequently diagnosed hematological malignancies. The expression of miR-582–3p was determined using
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Estrogen stimulates SREBP2 expression in hepatic cell lines via an estrogen response element in the SREBP2 promoter. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2019-12-03 Ye Meng,Lu Zong
Hypoestrogenism in women is strongly associated with menopause and it can lead to lipid disorder, which predisposes people to premature cardiovascular disease. However, the mechanism of lipid disorder remains unclear. Sterol regulatory element-binding protein 2 (SREBP2) is the key transcription factor regulating cholesterol metabolism. We hypothesize that estrogen regulates SREBP2 transcription through
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Cellular hypoxia promotes osteogenic differentiation of mesenchymal stem cells and bone defect healing via STAT3 signaling. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2019-12-03 Xin Yu,Qilong Wan,Xiaoling Ye,Yuet Cheng,Janak L Pathak,Zubing Li
Hypoxia in the vicinity of bone defects triggers the osteogenic differentiation of precursor cells and promotes healing. The activation of STAT3 signaling in mesenchymal stem cells (MSCs) has similarly been reported to mediate bone regeneration. However, the interaction between hypoxia and STAT3 signaling in the osteogenic differentiation of precursor cells during bone defect healing is still unknown
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Expression profiles of long noncoding RNAs and messenger RNAs in the border zone of myocardial infarction in rats. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2019-12-02 Qingkun Meng,Zhijun Sun,Hui Gu,Jiaying Luo,Jingjing Wang,Chuanhe Wang,Su Han
The participation of long noncoding RNAs (lncRNAs) in myocardial infarction has recently been noted. However, their underlying roles in the border zone of myocardial infarction remain unclear. This study uses microarrays to determine the profiles of lncRNAs and mRNAs in the border zone. Bioinformatics methods were employed to uncover their underlying roles. Highly dysregulated lncRNAs was further validated
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MicroRNA-146a protects against myocardial ischaemia reperfusion injury by targeting Med1. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2019-11-27 Tiantian Zhang,Yiwen Ma,Lin Gao,Chengyu Mao,Huasu Zeng,Xiaofei Wang,Yapin Sun,Jianmin Gu,Yue Wang,Kan Chen,Zhihua Han,Yuqi Fan,Jun Gu,Junfeng Zhang,Changqian Wang
Myocardial ischaemia reperfusion injury (MIRI) is a difficult problem in clinical practice, and it may involve various microRNAs. This study investigated the role that endogenous microRNA-146a plays in myocardial ischaemia reperfusion and explored the possible target genes. MIRI models were established in microRNA-146a deficient (KO) and wild type (WT) mice. MicroRNA-146a expression was evaluated in
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Down-regulation of miR-30b-5p protects cardiomyocytes against hypoxia-induced injury by targeting Aven. Cell. Mol. Biol. Lett. (IF 3.451) Pub Date : 2019-11-21 Lanfang Zhang,Xinwei Jia
Ischemia/hypoxia-induced cardiomyocyte apoptosis has been considered as a main cause of myocardial infarction. Here, we aimed to investigate the functional role of miR-30b-5p in hypoxic cardiomyocytes. AC16 human cardiomyocytes were cultured under hypoxia to simulate myocardial infarction. A qRT-PCR assay was performed to determine miR-30b-5p expression in hypoxic cardiomyocytes. Cell survival, injury