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Zinc finger protein 468 up-regulation of TFAM contributes to the malignant growth and cisplatin resistance of breast cancer cells Cell Div. (IF 2.3) Pub Date : 2024-03-01 Zhaoyang Jia, Feng Wang, Gongzhuo Li, Ping Jiang, Yuanxiu Leng, Longzhu Ke, Li Luo, Wei Gao
Because of the progress on the diagnosis and treatment for patients with breast cancer (BC), the overall survival of the patients has been improved. However, a number of BC patients cannot benefit from the existing therapeutic strategies as the essential molecular events triggering the development of BC are not well understood. Previous studies have shown that abnormal expression of zinc finger proteins
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Ultrasonic microbubbles promote mesenchymal stem cell homing to the fibrotic liver via upregulation of CXCR4 expression Cell Div. (IF 2.3) Pub Date : 2024-02-24 Heming Xu, Yize Huang, Fasu Zhang, Wei Shi, Yan Cheng, Kai Yang, Pingping Tian, Fei Zhou, Yuan Wang, Xueqing Fang, Youliang Song, Bo Liu, Liwei Liu
To investigate the mechanism of ultrasound microbubbles (UTMB) promoting stem cells homing to fibrotic liver. Bone marrow derived mesenchymal stem cells (BMSCs) were divided into 5 groups with or without ultrasound microbubbles and continuously irradiated with ultrasound conditions of frequency 1 MHZ and output power 0.6 W/cm2 for different times, and then injected into a mouse model of liver fibrosis
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Anti-cancer effects of Coix seed extract through KCTD9-mediated ubiquitination of TOP2A in lung adenocarcinoma Cell Div. (IF 2.3) Pub Date : 2024-02-20 Jiuyang Jiang, Xue Li, Chun Zhang, Jiafu Wang, Jin Li
Coix seed extract (CSE), a traditional Chinese medicine, has been reported as an adjunctive therapy in cancers. However, the molecular targets are largely unclear. The study is designed to unveil its function in lung adenocarcinoma (LUAD) and the possible molecular mechanism. The HERB database was utilized to predict the molecular targets of the Coix seed, followed by prognostic value prediction in
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Exosome-derived lncRNA A1BG-AS1 attenuates the progression of prostate cancer depending on ZC3H13-mediated m6A modification Cell Div. (IF 2.3) Pub Date : 2024-02-13 Zhi Yang, Yu Luo, Fan Zhang, Likun Ma
Exosome-derived long non-coding RNAs (lncRNAs) and N6-methyladenosine (m6A) modifications of lncRNAs have been shown crucial functions in prostate cancer (PCa). Herein, we aim to investigate the detailed mechanism of exosome-derived lncRNA A1BG-AS1 in PCa process. PCa cell exosomes were extracted, exosomal marker proteins (CD63, CD9) were detected utilizing western blotting, and exosomes with overexpressing
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Human Parathyroid Hormone (1–34) accelerates skin wound healing through inducing cell migration via up-regulating the expression of Rac1 Cell Div. (IF 2.3) Pub Date : 2024-02-12 Qingpeng Sun, Liya Zhou, Zhiyong Yu, Jun Zhang, Chao Zhang, Honglin Pi
Delayed wound healing is a public issue that imposes a significant burden on both society and the patients themselves. To date, although numerous methods have been developed to accelerate the speed of wound closure, the therapeutic effects are partially limited due to the complex procedures, high costs, potential side effects, and ethical concerns. While some studies have reported that the in-vivo
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Non-mitotic proliferation of malignant cancer cells revealed through live-cell imaging of primary and cell-line cultures Cell Div. (IF 2.3) Pub Date : 2024-02-10 Iram Shazia Tyagi, Ho Yin Calvin Tsui, Si Chen, Xinyi Li, Wai-Kin Mat, Muhammad A. Khan, Lucas Brendan Choy, Ka-Yin Aden Chan, Tat-Ming Danny Chan, Chi-Ping Stephanie Ng, Ho-Keung Ng, Wai Sang Poon, Hong Xue
Anti-mitosis has been a key strategy of anti-cancer therapies, targeting at a fundamental property of cancer cells, their non-controllable proliferation due to overactive mitotic divisions. For improved anti-cancer therapies, it is important to find out whether cancer cells can proliferate independent of mitosis and become resistant to anti-mitotic agents. In this study, live-cell imaging was applied
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Cilostazol protects against degenerative cervical myelopathy injury and cell pyroptosis via TXNIP-NLRP3 pathway Cell Div. (IF 2.3) Pub Date : 2024-01-17 Fei Xu, Zhuo Tian, Zhengguang Wang
Degenerative cervical myelopathy (DCM) is one of the most common and serious neurological diseases. Cilostazol has protective effects of anterior horn motor neurons and prevented the cell apoptosis. However, there was no literatures of Cilostazol on DCM. In this study, we established the DCM rat model to detect the effects of Cilostazol. Meanwhile, the neurobehavioral assessments, histopathology changes
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Role of reactive oxygen species in ultraviolet-induced photodamage of the skin Cell Div. (IF 2.3) Pub Date : 2024-01-12 Min Wei, Xin He, Na Liu, Hui Deng
Reactive oxygen species (ROS), such as superoxides (O2 •−) and hydroxyl groups (OH·), are short-lived molecules containing unpaired electrons. Intracellular ROS are believed to be mainly produced by the mitochondria and NADPH oxidase (NOX) and can be associated with various physiological processes, such as proliferation, cell signaling, and oxygen homeostasis. In recent years, many studies have indicated
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Jiedu Fuzheng decoction improves the proliferation, migration, invasion and EMT of non-small cell lung cancer via the Wnt/β-catenin pathway Cell Div. (IF 2.3) Pub Date : 2023-12-16 Fang Fang, Xiaowei Jin, Jinming Meng, Jiaqi He, Jiaxiao Wang, Changhong Wang, Sheng Xie, Wei Shi
This study aimed to investigate the effect of Jiedu Fuzheng decoction (JFD) in non-small cell lung cancer (NSCLC) and its potential therapeutic mechanism. We prepared JFD-medicated serum from rats and treated NSCLC cells (A549 and NCI-H1650) with 0.5, 1, and 2 mg/mL JFD-medicated serum. CCK-8 and colony formation assays were used to detect cell proliferation. Transwell assays showed that JFD attenuated
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Downregulation of GDF15 suppresses ferroptosis and predicts unfavorable prognosis in clear cell renal cell carcinoma Cell Div. (IF 2.3) Pub Date : 2023-12-11 Dongliang Yang, Zhongyin He, Jiawei Lu, Xiaolin Yuan, Haiyong Liu, Yagang Xue, Ting Chen, Hongxing Gu
Growth differentiation factor 15 (GDF15), a member of the transforming growth factor beta (TGF-β) superfamily, is involved in various pathophysiological processes such as anorexia, obesity, inflammation, and tumorigenesis. However, the role of GDF15 in clear cell renal cell carcinoma (ccRCC) remains poorly understood. Clinical significance of GDF15 in ccRCC as well as other types of human cancers was
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Exosome-transmitted miR-30a-5p enhances cell proliferation, migration, and invasion in ovarian cancer Cell Div. (IF 2.3) Pub Date : 2023-11-01 Jian Cao, Huan Wang, Jing Yang, Jing Zhang, Dake Li, Pengfei Xu
Ovarian cancer (OC) causes the highest rates of mortality among women’s genital tract malignancies. Micro-ribonucleic acid (miRNA), the most abundant long noncoding RNAs transmitted by exosomes, has been revealed to be a potential marker for OC since 2008. In this study, we aimed to determine the possible roles of miRNAs derived from exosomes in the early diagnosis of OC through miRNA microarray, besides
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Angelica acutiloba Kitagawa flower induces A549 cell pyroptosis via the NF-κB/NLRP3 pathway for anti-lung cancer effects Cell Div. (IF 2.3) Pub Date : 2023-11-01 Yonghu Chen, Fangying Zhu, Xianhua Che, Yanwei Li, Ning Li, Zhe Jiang, Xuezheng Li
Angelica acutiloba Kitagawa, a traditional medicinal herb of the Umbelliferae family, has been demonstrated to have anticancer activity. In this study, we investigated the anti-lung cancer effects of two compounds extracted from A. acutiloba flowers: kaempferol-3-O-α-L-(4″-E-p-coumaroyl)-rhamnoside (KAE) and platanoside (PLA). MTT, cell colony formation, and cell migration (scratch) assays revealed
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Polyploid cell dynamics and death before and after PEG-treatment of a NIH/3T3 derived culture: vinblastine effects on the regulation of cell subpopulations heterogeneity Cell Div. (IF 2.3) Pub Date : 2023-10-30 Alessandra Spano, Luigi Sciola
Neoplastic subpopulations can include polyploid cells that can be involved in tumor evolution and recurrence. Their origin can be traced back to the tumor microenvironment or chemotherapeutic treatment, which can alter cell division or favor cell fusion, generating multinucleated cells. Their progeny, frequently genetically unstable, can result in new aggressive and more resistant to chemotherapy subpopulations
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Preeclampsia-associated lncRNA FEZF1-AS1 regulates cell proliferation and apoptosis in placental trophoblast cells through the ELAVL1/NOC2L axis Cell Div. (IF 2.3) Pub Date : 2023-10-23 Xudong Zhao, Fengyun Su, Qing Guo, Xiuhong Tao, Huifeng Wang, Hongling Wang, Qinwen Li, Wangmeng Zhang
LncRNAs have been shown to be involved in and control the biological processes of multiple diseases, including preeclampsia (PE). The impairment of trophoblast cell proliferation is recognized as a significant anomaly contributing to the development of PE. LncRNA FEZF1-AS1 was found downregulated in placental tissues of PE patients. However, the precise regulatory mechanism of FEZF1-AS1 in placental
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Slik maintains tissue homeostasis by preventing JNK-mediated apoptosis Cell Div. (IF 2.3) Pub Date : 2023-10-04 Chenglin Li, Xiaojie Zhu, Xinyue Sun, Xiaowei Guo, Wenzhe Li, Ping Chen, Yulii V. Shidlovskii, Qian Zhou, Lei Xue
The c-Jun N-terminal kinase (JNK) pathway is an evolutionarily conserved regulator of cell death, which is essential for coordinating tissue homeostasis. In this study, we have characterized the Drosophila Ste20-like kinase Slik as a novel modulator of JNK pathway-mediated apoptotic cell death. First, ectopic JNK signaling-triggered cell death is enhanced by slik depletion whereas suppressed by Slik
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Kynureninase knockdown inhibits cisplatin resistance in vivo and in vitro and impacts the prognosis of cervical adenocarcinoma Cell Div. (IF 2.3) Pub Date : 2023-09-24 Jun-wen Zhang, Ya-nan Wang, Mei-ling Zhong, Mei-rong Liang
Chemotherapy resistance is a leading cause of treatment failure in cases of cervical adenocarcinoma (ADC), and no effective treatment approach has yet been found. We previously identified the differentially expressed kynureninase (KYNU) mRNA in cervical adenocarcinoma cells (HeLa) and cervical adenocarcinoma cisplatin resistance cells (HeLa/DDP) using gene chips. However, the role and potential mechanism
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Rapid and accurate genotyping of human SNP rs671 in aldehyde dehydrogenase 2 gene using one-step CRISPR/Cas12b assay without DNA amplification Cell Div. (IF 2.3) Pub Date : 2023-08-28 Fang Wu, Yong Xue, Yan Wang, Xinxin Si, Xinyue Zhang, Yuyang Xu, Zhidan Luo
The SNP rs671 of Human aldehyde dehydrogenase (ALDH) is G-A transition at 1510th nucleotides, which is an important clinical indicator of alcoholic liver disease, digestive tract cancer and some drug efficiency. The commonly used genotyping assay of this polymorphism is relatively time-consuming and costly. This study develops a rapid and accurate one-step CRISPR/Cas12b assay to distinguish the G1510A
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The roles of METTL3 on autophagy and proliferation of vascular smooth muscle cells are mediated by mTOR rather than by CDK1 Cell Div. (IF 2.3) Pub Date : 2023-08-09 Hanshen Luo, Xingliang Wu, Bo Huo, Liyuan Liu, Ding-Sheng Jiang, Xin Yi
Aberrant proliferation of vascular smooth muscle cells (VSMCs) is the cause of neointima formation followed by vascular injury. Autophagy is involved in this pathological process, but its function is controversial. Recently, we found that methyltransferase like 3 (METTL3) inhibited VSMC proliferation by activating autophagosome formation. Moreover, we also demonstrated that METTL3 reduced the levels
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Expression of genes regulating cell division in porcine follicular granulosa cells Cell Div. (IF 2.3) Pub Date : 2023-08-07 Jakub Kulus, Wiesława Kranc, Magdalena Kulus, Piotr Dzięgiel, Dorota Bukowska, Paul Mozdziak, Bartosz Kempisty, Paweł Antosik
Cell cycle regulation influences the proliferation of granulosa cells and affects many processes related to ovarian folliclular growth and ovulation. Abnormal regulation of the cell cycle can lead to many diseases within the ovary. The aim of this study was to describe the expression profile of genes within granulosa cells, which are related to the formation of the cytoskeleton, organization of cell
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Role of microRNAs in regulation of doxorubicin and paclitaxel responses in lung tumor cells Cell Div. (IF 2.3) Pub Date : 2023-07-21 Amirhosein Maharati, Meysam Moghbeli
Lung cancer as the leading cause of cancer related mortality is always one of the main global health challenges. Despite the recent progresses in therapeutic methods, the mortality rate is still significantly high among lung cancer patients. A wide range of therapeutic methods including chemotherapy, radiotherapy, and surgery are used to treat lung cancer. Doxorubicin (DOX) and Paclitaxel (TXL) are
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Development and validation of a two glycolysis-related LncRNAs prognostic signature for glioma and in vitro analyses Cell Div. (IF 2.3) Pub Date : 2023-06-24 Xiaoping Xu, Shijun Zhou, Yuchuan Tao, Zhenglan Zhong, Yongxiang Shao, Yong Yi
Mounting evidence suggests that there is a complex regulatory relationship between long non-coding RNAs (lncRNAs) and the glycolytic process during glioma development. This study aimed to investigate the prognostic role of glycolysis-related lncRNAs in glioma and their impact on the tumor microenvironment. This study utilized glioma transcriptome data from public databases to construct, evaluate, and
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SIRT4 is an independent prognostic factor in bladder cancer and inhibits bladder cancer growth by suppressing autophagy Cell Div. (IF 2.3) Pub Date : 2023-06-10 Jie Yin, Guohao Cai, Huaiwen Wang, Weijia Chen, Shan Liu, Guoyu Huang
Nucleosome-localized sirtuin 4 (SIRT4) was found to function as an oncogene and tumor suppressor gene in different tumors. However, the clinical significance of SIRT4 in bladder urothelial carcinoma (BLCA) has not been assessed, nor has the function of SIRT4 in BLCA been analyzed. In this study, we assessed the levels of SIRT4 protein in BLCA tissues and its association with clinicopathological parameters
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Molecular mechanisms involved in regulating protein activity and biological function of MST3 Cell Div. (IF 2.3) Pub Date : 2023-05-18 Jing Qiu, Junzhi Xiong, Lu Jiang, Xinmin Wang, Kebin Zhang, Hua Yu
Mammalian sterile 20-like (Ste20-like) protein kinase 3 (MST3) or serine/threonine-protein kinase 24 (STK24) is a serine/threonine protein kinase that belongs to the mammalian STE20-like protein kinase family. MST3 is a pleiotropic protein that plays a critical role in regulating a variety of events, including apoptosis, immune response, metabolism, hypertension, tumor progression, and development
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circRNA_0001006 predicts prognosis and regulates cellular processes of triple-negative breast cancer via miR-424-5p Cell Div. (IF 2.3) Pub Date : 2023-05-16 Jiaqi Liu, Linna Kong, Wenqing Bian, Xiaona Lin, Feifei Wei, Jun Chu
circular RNAs (circRNAs) have been considered novel biomarker candidates for human cancers, such as triple-negative breast cancer (TNBC). circ_0001006 was identified as a differentially expressed circRNA in metastatic breast cancer, but its significance and function in TNBC were unclear. The significance of circ_0001006 in TNBC was assessed and exploring its potential molecular mechanism to provide
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Sustained Shugoshin 1 downregulation reduces tumor growth and metastasis in a mouse xenograft tumor model of triple-negative breast cancer Cell Div. (IF 2.3) Pub Date : 2023-04-30 Shirley Jusino, Yainyrette Rivera-Rivera, Camille Chardón-Colón, Patricia C. Rodríguez-Rodríguez, Janeishly Román-González, Valeria S. Juliá-Hernández, Angel Isidro, Qianxing Mo, Harold I. Saavedra
Triple-negative breast cancer (TBNC) is an aggressive breast cancer subtype with a poor prognosis. Shugoshin-1 (SGO1) protects chromatids from early separation. Previous studies from our group have demonstrated that transient SGO1 downregulation suppresses early stages of metastasis (the epithelial-to-mesenchymal transition, or EMT, cell invasion, and cell migration) in TNBC cells. Thus, the inhibition
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Update of cellular responses to the efferocytosis of necroptosis and pyroptosis Cell Div. (IF 2.3) Pub Date : 2023-04-09 Chandra Agung Purnama, Anna Meiliana, Melisa Intan Barliana, Keri Lestari
Cell death is a basic physiological process that occurs in all living organisms. A few key players in these mechanisms, as well as various forms of cell death programming, have been identified. Apoptotic cell phagocytosis, also known as apoptotic cell clearance, is a well-established process regulated by a number of molecular components, including ‘find-me’, ‘eat-me’ and engulfment signals. Efferocytosis
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MiR-146b-5p/SEMA3G regulates epithelial-mesenchymal transition in clear cell renal cell carcinoma Cell Div. (IF 2.3) Pub Date : 2023-03-07 Mengxi Tang, Tao Xiong
The primary purpose was to unveil how the miR-146b-5p/SEMA3G axis works in clear cell renal cell carcinoma (ccRCC). ccRCC dataset was acquired from TCGA database, and target miRNA to be studied was further analyzed using survival analysis. We performed miRNA target gene prediction through the database, and those predicted miRNAs were intersected with differential mRNAs. After calculating the correlation
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Network regulatory mechanism of ncRNA on the Wnt signaling pathway in osteoporosis Cell Div. (IF 2.3) Pub Date : 2023-03-07 Fangyu An, Xiangrui Meng, Lingqing Yuan, Yanqiang Niu, Jie Deng, Zhaohui Li, Yongqi Liu, Ruoliu Xia, Shiqing Liu, Chunlu Yan
Non-coding RNA (ncRNA) is a type of non-protein-coding RNA molecule transcribed from the genome which performs broad regulation of a variety of biological functions in human cells. The Wnt signaling pathway is highly conserved in multicellular organisms, playing an important role in their growth and development. Increasing evidence suggests that ncRNA can regulate cell biological function, enhance
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Commentary: locating the restriction point Cell Div. (IF 2.3) Pub Date : 2023-02-10 Robert F. Brooks
Attempts to map the Restriction Point in the mammalian cell cycle typically involve stimulating quiescent cells with mitogens for increasing intervals, removing the stimulus and then determining the proportion of cells that reach S phase at some point later. This “fixed point” estimate assumes that further cell cycle commitment ceases as soon as the stimulus is removed. In fact, kinetic analysis shows
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Circular RNA hsa_circ_0075323 promotes glioblastoma cells proliferation and invasion via regulation of autophagy Cell Div. (IF 2.3) Pub Date : 2023-01-17 Zhang, Wenrui, Shi, Zhonggang, Chen, Shouren, Shen, Shaoshan, Tu, Songjie, Yang, Jian, Qiu, Yongming, Lin, Yingying, Dai, Xuejun
Protein p62 (sequestosome 1) encoded by gene SQSTM1 plays a vital role in mediating protectively selective autophagy in tumor cells under stressed conditions. CircSQSTM1 (hsa_circ_0075323) is a circular transcript generated from gene SQSTM1 (chr5:179260586–179260782) by back-splicing. However, the potential role of hsa_hsa_circ_0075323 in glioblastoma (GBM) remains unclear. Here, we aimed to explore
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Ethnic and racial-specific differences in levels of centrosome-associated mitotic kinases, proliferative and epithelial-to-mesenchymal markers in breast cancers Cell Div. (IF 2.3) Pub Date : 2022-12-09 Rivera-Rivera, Yainyrette, Vargas, Geraldine, Jaiswal, Neha, Núñez-Marrero, Angel, Li, Jiannong, Chen, Dung-Tsa, Eschrich, Steven, Rosa, Marilin, Johnson, Joseph O., Dutil, Julie, Chellappan, Srikumar P., Saavedra, Harold I.
Molecular epidemiology evidence indicates racial and ethnic differences in the aggressiveness and survival of breast cancer. Hispanics/Latinas (H/Ls) and non-Hispanic Black women (NHB) are at higher risk of breast cancer (BC)-related death relative to non-Hispanic white (NHW) women in part because they are diagnosed with hormone receptor-negative (HR) subtype and at higher stages. Since the cell cycle
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The genomic stability regulator PTIP is required for proper chromosome segregation in mitosis Cell Div. (IF 2.3) Pub Date : 2022-09-24 Zhang, Fengxia, Wei, Mingxuan, Chen, Haoran, Ji, Liting, Nie, Yan, Kang, Jungseog
The Pax transcription activation domain-interacting protein (PTIP) is a nuclear protein that is an essential component of H3K4 methylation for gene activation in vascular, kidney, B cell, and adipocyte development. Furthermore, it plays a key role in genomic stability in higher eukaryotic cells. It binds to 53BP1 and antagonizes inappropriate homologous recombination for a proper DNA damage response
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dGLYAT modulates Gadd45-mediated JNK activation and cell invasion Cell Div. (IF 2.3) Pub Date : 2022-08-06 Xu, Meng, Ren, Pu, Tian, Juhui, Xiao, Lisha, Hu, Ping, Chen, Ping, Li, Wenzhe, Xue, Lei
Cell invasion is a crucial step of tumor metastasis, finding new regulators of which offers potential drug targets for cancer therapy. Aberrant GLYAT expression is associated with human cancers, yet its role in cancer remains unknown. This study aims to understand the function and mechanism of Drosophila GLYAT in cell invasion. We found that dGLYAT regulates Gadd45-mediated JNK pathway activation and
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Proteolysis dependent cell cycle regulation in Caulobacter crescentus Cell Div. (IF 2.3) Pub Date : 2022-04-01 Fatima, Nida I, Fazili, Khalid Majid, Bhat, Nowsheen Hamid
Caulobacter crescentus, a Gram-negative alpha-proteobacterium, has surfaced as a powerful model system for unraveling molecular networks that control the bacterial cell cycle. A straightforward synchronization protocol and existence of many well-defined developmental markers has allowed the identification of various molecular circuits that control the underlying differentiation processes executed at
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Principles of dormancy evident in high-grade serous ovarian cancer Cell Div. (IF 2.3) Pub Date : 2022-03-23 Shepherd, Trevor G., Dick, Frederick A.
In cancer, dormancy refers to a clinical state in which microscopic residual disease becomes non-proliferative and is largely refractory to chemotherapy. Dormancy was first described in breast cancer where disease can remain undetected for decades, ultimately leading to relapse and clinical presentation of the original malignancy. A long latency period can be explained by withdrawal from cell proliferation
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An updated view on the centrosome as a cell cycle regulator Cell Div. (IF 2.3) Pub Date : 2022-02-14 Lin, Muyang, Xie, Shuang Shuang, Chan, Kuan Yoow
The centrosome is a multifunctional organelle that is known primarily for its microtubule organising function. Centrosomal defects caused by changes in centrosomal structure or number have been associated with human diseases ranging from congenital defects to cancer. We are only beginning to appreciate how the non-microtubule organising roles of the centrosome are related to these clinical conditions
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Heat shock factor 1 suppression induces spindle abnormalities and sensitizes cells to antimitotic drugs Cell Div. (IF 2.3) Pub Date : 2021-12-18 Kuo, Hsiao-Hui, Su, Zhi-Rou, Chuang, Jing-Yuan, Yih, Ling-Huei
Heat shock factor 1 (HSF1) is the master regulator of the heat shock response and supports malignant cell transformation. Recent work has shown that HSF1 can access the promoters of heat shock proteins (HSPs) and allow HSP expression during mitosis. It also acts as a mitotic regulator, controlling chromosome segregation. In this study, we investigated whether the transactivation activity of HSF1 is
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ROMO1 is required for mitochondrial metabolism during preimplantation embryo development in pigs Cell Div. (IF 2.3) Pub Date : 2021-12-16 Zhou, Dongjie, Sun, Ming-Hong, Lee, Song-Hee, Cui, Xiang-Shun
Reactive oxygen species (ROS) modulator 1 (ROMO1) is a mitochondrial membrane protein that is essential for the regulation of mitochondrial ROS production and redox sensing. ROMO1 regulates ROS generation within cells and is involved in cellular processes, such as cell proliferation, senescence, and death. Our purpose is to investigates the impact of ROMO1 on the mitochondria during porcine embryogenesis
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MPS1 is involved in the HPV16-E7-mediated centrosomes amplification Cell Div. (IF 2.3) Pub Date : 2021-11-04 Alfaro-Mora, Yair, Domínguez-Gómez, Guadalupe, Cáceres-Gutiérrez, Rodrigo E., Tolentino-García, Laura, Herrera, Luis A., Castro-Hernández, Clementina, Bermúdez-Cruz, Rosa María, Díaz-Chávez, José
It has been reported that the oncoprotein E7 from human papillomavirus type 16 (HPV16-E7) can induce the excessive synthesis of centrosomes through the increase in the expression of PLK4, which is a transcriptional target of E2F1. On the other hand, it has been reported that increasing MPS1 protein stability can also generate an excessive synthesis of centrosomes. In this work, we analyzed the possible
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Asymmetric cell division of mammary stem cells Cell Div. (IF 2.3) Pub Date : 2021-09-29 Chhabra, Shaan N., Booth, Brian W.
Somatic stem cells are distinguished by their capacity to regenerate themselves and also to produce daughter cells that will differentiate. Self-renewal is achieved through the process of asymmetric cell division which helps to sustain tissue morphogenesis as well as maintain homeostasis. Asymmetric cell division results in the development of two daughter cells with different fates after a single mitosis
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The budding yeast protein Chl1p is required for delaying progression through G1/S phase after DNA damage Cell Div. (IF 2.3) Pub Date : 2021-09-08 Katheeja, Muhseena N., Das, Shankar Prasad, Laha, Suparna
The budding yeast protein Chl1p is a nuclear protein required for sister-chromatid cohesion, transcriptional silencing, rDNA recombination, ageing and plays an instrumental role in chromatin remodeling. This helicase is known to preserve genome integrity and spindle length in S-phase. Here we show additional roles of Chl1p at G1/S phase of the cell cycle following DNA damage. G1 arrested cells when
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Ral GTPase is essential for actin dynamics and Golgi apparatus distribution in mouse oocyte maturation Cell Div. (IF 2.3) Pub Date : 2021-06-10 Ming-Hong Sun, Lin-Lin Hu, Chao-Ying Zhao, Xiang Lu, Yan-Ping Ren, Jun-Li Wang, Xiang-Shun Cui, Shao-Chen Sun
Ral family is a member of Ras-like GTPase superfamily, which includes RalA and RalB. RalA/B play important roles in many cell biological functions, including cytoskeleton dynamics, cell division, membrane transport, gene expression and signal transduction. However, whether RalA/B involve into the mammalian oocyte meiosis is still unclear. This study aimed to explore the roles of RalA/B during mouse
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Mitotic arrest affects clustering of tumor cells Cell Div. (IF 2.3) Pub Date : 2021-01-29 Julia Bonnet, Lise Rigal, Odile Mondesert, Renaud Morin, Gaëlle Corsaut, Mathieu Vigneau, Bernard Ducommun, Valérie Lobjois
Cancer cell aggregation is a key process involved in the formation of tumor cell clusters. It has recently been shown that clusters of circulating tumor cells (CTCs) have an increased metastatic potential compared to isolated circulating tumor cells. Several widely used chemotherapeutic agents that target the cytoskeleton microtubules and cause cell cycle arrest at mitosis have been reported to modulate
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Microarray expression profile of mRNAs and long noncoding RNAs and the potential role of PFK-1 in infantile hemangioma Cell Div. (IF 2.3) Pub Date : 2021-01-11 Kaiying Yang, Xuepeng Zhang, Linwen Chen, Siyuan Chen, Yi Ji
Infantile hemangioma (IH) is the most common benign tumor in children. Long noncoding RNAs (lncRNAs) play a critical role in tumorigenesis. However, the expression levels and biological functions of lncRNAs in IH have not been well-studied. This study aimed to analyze the expression profile of lncRNAs and mRNAs in proliferating and involuting IHs. The expression profiles of lncRNAs and mRNAs in proliferating
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Chromosomal aberration arises during somatic reprogramming to pluripotent stem cells Cell Div. (IF 2.3) Pub Date : 2020-11-03 Xinyu Liu, Conghui Li, Kang Zheng, Xiaofeng Zhao, Xiaofeng Xu, Aifen Yang, Min Yi, Huaping Tao, Binghua Xie, Mengsheng Qiu, Junlin Yang
Reprogramming somatic cells to induced pluripotent stem cells (iPSCs) has opened new therapeutic possibilities. However, karyotypic abnormalities detected in iPSCs compromised their utility, especially chromosomal aberrations found at early passages raised serious safety concerns. The mechanism underlying the chromosomal abnormality in early-passage iPSCs is not known. Human dermal fibroblasts (HDFs)
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XMU-MP-1 induces growth arrest in a model human mini-organ and antagonises cell cycle-dependent paclitaxel cytotoxicity Cell Div. (IF 2.3) Pub Date : 2020-09-17 Ellen Mitchell, Charlotte E. L. Mellor, Talveen S. Purba
XMU-MP-1 is an inhibitor of the Hippo pathway kinases MST1/2 and has been shown to promote the downstream activation of the pro-proliferative, pro-regenerative and anti-apoptotic transcriptional regulator YAP1. We tested whether XMU-MP-1 can activate YAP1 in a model human mini-organ, namely the hair follicle, to determine whether it can be pharmacologically exploited to promote regeneration in the
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Caffeine as a tool for investigating the integration of Cdc25 phosphorylation, activity and ubiquitin-dependent degradation in Schizosaccharomyces pombe. Cell Div. (IF 2.3) Pub Date : 2020-06-29 John P Alao,Per Sunnerhagen
The evolutionarily conserved Cdc25 phosphatase is an essential protein that removes inhibitory phosphorylation moieties on the mitotic regulator Cdc2. Together with the Wee1 kinase, a negative regulator of Cdc2 activity, Cdc25 is thus a central regulator of cell cycle progression in Schizosaccharomyces pombe. The expression and activity of Cdc25 is dependent on the activity of the Target of Rapamycin
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The G2-to-M transition from a phosphatase perspective: a new vision of the meiotic division. Cell Div. (IF 2.3) Pub Date : 2020-05-25 Tom Lemonnier,Aude Dupré,Catherine Jessus
Cell division is orchestrated by the phosphorylation and dephosphorylation of thousands of proteins. These post-translational modifications underlie the molecular cascades converging to the activation of the universal mitotic kinase, Cdk1, and entry into cell division. They also govern the structural events that sustain the mechanics of cell division. While the role of protein kinases in mitosis has
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EZH2-inhibitor DZNep enhances apoptosis of renal tubular epithelial cells in presence and absence of cisplatin. Cell Div. (IF 2.3) Pub Date : 2020-05-25 Si-Qi Chen,Jia-Qi Li,Xiao-Qiao Wang,Wen-Jing Lei,Hao Li,Jiao Wan,Zheng Hu,Yao-Wei Zou,Xiao-Yu Wu,Hong-Xin Niu
The enhancer of zeste homolog 2 (EZH2) is a histone methyltransferase and induces the trimethylation of histone H3 lysine 27 (H3K27me3) in the promoter of many key genes; EZH2 acts as a transcriptional repressor and is an epigenetic regulator for several cancers. However, the role of EZH2 in nonneoplastic diseases, such as kidney diseases, is unknown and has been investigated. NRK-52E cells were treated
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Toll signaling promotes JNK-dependent apoptosis in Drosophila Cell Div. (IF 2.3) Pub Date : 2020-03-10 Zhuojie Li, Chenxi Wu, Xiang Ding, Wenzhe Li, Lei Xue
Apoptosis plays pivotal roles in organ development and tissue homeostasis, with its major function to remove unhealthy cells that may compromise the fitness of the organism. Toll signaling, with the ancient evolutionary origin, regulates embryonic dorsal–ventral patterning, axon targeting and degeneration, and innate immunity. Using Drosophila as a genetic model, we characterized the role of Toll signaling
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Kinesin-5 Eg5 is essential for spindle assembly and chromosome alignment of mouse spermatocytes. Cell Div. (IF 2.3) Pub Date : 2020-03-06 Zhen-Yu She,Ning Zhong,Kai-Wei Yu,Yu Xiao,Ya-Lan Wei,Yang Lin,Yue-Ling Li,Ming-Hui Lu
Background Microtubule organization is essential for bipolar spindle assembly and chromosome segregation, which contribute to genome stability. Kinesin-5 Eg5 is known to be a crucial regulator in centrosome separation and spindle assembly in mammalian somatic cells, however, the functions and mechanisms of Eg5 in male meiotic cell division remain largely unknown. Results In this study, we have found
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IGFBP5 increases cell invasion and inhibits cell proliferation by EMT and Akt signaling pathway in Glioblastoma multiforme cells. Cell Div. (IF 2.3) Pub Date : 2020-02-27 Chengyuan Dong,Junwen Zhang,Sheng Fang,Fusheng Liu
Background Recurrence of Glioblastoma multiforme (GBM) seems to be the rule despite combination therapies. Cell invasion and cell proliferation are major reasons for recurrence of GBM. And insulin-like growth factor binding protein 5 (IGFBP5) is the most conserved of the IGFBPs and is frequently dysregulated in cancers and metastatic tissues. Results By studying the human glioma tissues, we find that
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A positive feedback loop between EZH2 and NOX4 regulates nucleus pulposus cell senescence in age-related intervertebral disc degeneration. Cell Div. (IF 2.3) Pub Date : 2020-02-01 Chang Liu,Libangxi Liu,Minghui Yang,Bin Li,Jiarong Yi,Xuezheng Ai,Yang Zhang,Bo Huang,Changqing Li,Chencheng Feng,Yue Zhou
Background The senescence of nucleus pulposus (NP) cells plays a vital role in the pathogenesis of intervertebral disc (IVD) degeneration (IDD). NADPH oxidase 4 (NOX4)-associated oxidative stress has been shown to induce premature NP cell senescence. Enhancer of zeste homolog 2 (EZH2) is a crucial gene regulating cell senescence. The aim of this study was to investigate the roles of EZH2 in NOX4-induced
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Coupled cycling and regulation of metazoan morphogenesis. Cell Div. (IF 2.3) Pub Date : 2020-01-27 Saba Rezaei-Lotfi,Ramin M Farahani
Metazoan animals are characterized by restricted phenotypic heterogeneity (i.e. morphological disparity) of organisms within various species, a feature that contrasts sharply with intra-species morphological diversity observed in the plant kingdom. Robust emergence of morphogenic blueprint in metazoan animals reflects restricted autonomy of individual cells in adoption of fate outcomes such as differentiation
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The essentiality landscape of cell cycle related genes in human pluripotent and cancer cells Cell Div. (IF 2.3) Pub Date : 2019-12-23 Ruth Viner-Breuer, Atilgan Yilmaz, Nissim Benvenisty, Michal Goldberg
Cell cycle regulation is a complex system consisting of growth-promoting and growth-restricting mechanisms, whose coordinated activity is vital for proper division and propagation. Alterations in this regulation may lead to uncontrolled proliferation and genomic instability, triggering carcinogenesis. Here, we conducted a comprehensive bioinformatic analysis of cell cycle-related genes using data from
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Mechanisms for the temporal regulation of substrate ubiquitination by the anaphase-promoting complex/cyclosome Cell Div. (IF 2.3) Pub Date : 2019-12-23 Shivangee Bansal, Swati Tiwari
The anaphase-promoting complex/cyclosome (APC/C) is a multi-subunit, multifunctional ubiquitin ligase that controls the temporal degradation of numerous cell cycle regulatory proteins to direct the unidirectional cell cycle phases. Several different mechanisms contribute to ensure the correct order of substrate modification by the APC/C complex. Recent advances in biochemical, biophysical and structural
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FGF-induced LHX9 regulates the progression and metastasis of osteosarcoma via FRS2/TGF-β/β-catenin pathway. Cell Div. (IF 2.3) Pub Date : 2019-11-25 Shuang-Qing Li,Chao Tu,Lu Wan,Rui-Qi Chen,Zhi-Xi Duan,Xiao-Lei Ren,Zhi-Hong Li
Background Fibroblast growth factor (FGF) and tumor growth factor-β (TGFβ) have emerged as pivotal regulators during the progression of osteosarcoma (OS). LHX9 is one crucial transcription factor controlled by FGF, however, its function in OS has not been investigated yet. Methods The expression of LHX9, FRS2, BMP4, TGF-beta R1, SMAD2, beta-catenin and metastasis-related proteins was measured by real-time
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CRY arrests Cop1 to regulate circadian rhythms in mammals. Cell Div. (IF 2.3) Pub Date : 2019-11-02 Choogon Lee
Cryptochromes (CRYs) are UVA and blue light photoreceptors present in all major evolutionary lineages ranging from cyanobacteria to plants and animals, including mammals. In plants, blue light activates CRYs to induce photomorphogenesis by inhibiting the CRL4Cop1 E3 ligase complex which regulates the degradation of critical transcription factors involved in plant development and growth. However, in
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MicroRNAs' control of cancer cell dormancy. Cell Div. (IF 2.3) Pub Date : 2019-10-10 Tatiana G Ruksha
'Dormancy', in the context of carcinogenesis, is a biological phenomenon of decreased cancer cell proliferation and metabolism. In view of their ability to remain quiescent, cancer cells are able to avoid cell death induced by chemotherapeutic agents, and thereby give rise to tumor relapse at a later stage. Being a dynamic event, the dormant state is controlled by several epigenetic mechanisms, including
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Correction to: Reactivation of TAp73 tumor suppressor by protoporphyrin IX, a metabolite of aminolevulinic acid, induces apoptosis in TP53-deficient cancer cells. Cell Div. (IF 2.3) Pub Date : 2019-08-29 Alicja Sznarkowska,Anna Kostecka,Anna Kawiak,Pilar Acedo,Mattia Lion,Alberto Inga,Joanna Zawacka-Pankau
[This corrects the article DOI: 10.1186/s13008-018-0043-3.].