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The aging proteostasis decline: From nematode to human Exp. Cell Res. (IF 3.383) Pub Date : 2021-01-09 Anatoly Meller; Reut Shalgi
The aging proteostasis decline manifests in a failure of aging cells and organisms to properly respond to proteotoxic challenges. This proteostasis collapse has long been considered a hallmark of aging in nematodes, and has recently been shown to occur also in human cells upon entry to senescence, opening the way to exploring the phenomenon in the broader context of human aging. Cellular senescence
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Cultured cardiac fibroblasts and myofibroblasts express Sushi Containing Domain 2 and assemble a unique fibronectin rich matrix Exp. Cell Res. (IF 3.383) Pub Date : 2021-01-14 Eric G. Schmuck; Sushmita Roy; Anisa Dhillon; Sydney Walker; Keith Spinali; Sophia Colevas; Tianhua Zhou; Gagan Chhabra; Yuming Liu; Md Abdul Kader Sagar; Charlie J. Childs; John A. Kink; Kevin W. Eliceiri; Peiman Hematti; Amish N. Raval
Cardiac fibroblasts and myofibroblasts assemble and maintain extracellular matrix during normal development and following injury. Culture expansion of these cells yield a bioengineered matrix that could lead to intriguing therapeutic opportunities. For example, we reported that cultured rat cardiac fibroblasts form a matrix that can be used to delivery therapeutic stem cells. Furthermore, we reported
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Functional proteoliposome-like structure derived from simultaneous evisceration and enucleation of T-lymphoblastoid A3R5.7 cells: a Top-Down story Exp. Cell Res. (IF 3.383) Pub Date : 2021-01-18 Cherng-Wen Darren Tan; Andreas Forsthuber; Eva-Kathrin Ehmoser
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Down-regulation of A3AR signaling by IL-6-induced GRK2 activation contributes to Th17 cell differentiation Exp. Cell Res. (IF 3.383) Pub Date : 2021-01-09 Shanshan Hu; Paipai Guo; Zhen Wang; Zhengwei Zhou; Rui Wang; Mei Zhang; Juan Tao; Yu Tai; Weijie Zhou; Wei Wei; Qingtong Wang
IL-6-triggered Th17 cell expansion is responsible for the pathogenesis of many immune diseases including rheumatoid arthritis (RA). Traditionally, IL-6 induces Th17 cell differentiation through JAK-STAT3 signaling. In the present work, PKA inhibition reduces in vitro induction of Th17 cells, while IL-6 stimulation of T cells facilitates the internalization of A3AR and increased cAMP production in a
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Immunophenotypic characterization and therapeutics effects of human bone marrow- and umbilical cord-derived mesenchymal stromal cells in an experimental model of sepsis Exp. Cell Res. (IF 3.383) Pub Date : 2021-01-08 Amir K. Varkouhi; Xiaolin He; Ana Paula Teixeira Monteiro; Hajera Amatullah; James N. Tsoporis; Sahil Gupta; Amin M. Ektesabi; Shirley H.J. Mei; Duncan J. Stewart; Armand Keating; Claudia C. dos Santos
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Myeloid NEMO deficiency promotes tumor immunosuppression partly via MCP1-CCR2 axis Exp. Cell Res. (IF 3.383) Pub Date : 2021-01-08 Li Yuanyuan; Liu Yakun; Li Zhongyao; Yi Le; Duan Weisong; Guo Moran; Bu Hui; Li Chunyan
Tumor-associated macrophages (TAM), which are found in the tumor microenvironment of solid tumors, not only mediate cancer immune evasion but also promote tumor growth. The transcription factor NF-κB, which is a crucial link between inflammation and tumors, can accelerate tumor occurrence and development. NEMO, the regulatory subunit of the IKK complex, plays a pivotal role in activating the NF-κB
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J-domain proteins interaction with neurodegenerative disease-related proteins Exp. Cell Res. (IF 3.383) Pub Date : 2021-01-16 Sara Maria Ayala Mariscal; Janine Kirstein
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Endothelial cells control vascular smooth muscle cell cholesterol levels by regulating 24-dehydrocholesterol reductase expression Exp. Cell Res. (IF 3.383) Pub Date : 2021-01-07 Johanna Kohlhaas; Marius Andreas Jäger; Leandra Lust; Carolina De La Torre; Markus Hecker; Thomas Korff
Communication of vascular cells is essential for the control of organotypic functions of blood vessels. In this context, vascular endothelial cells (EC) act as potent regulators of vascular smooth muscle cell (VSMC) functions such as contraction and relaxation. However, the impact of ECs on the gene expression pattern of VSMCs is largely unknown. Here, we investigated changes of the VSMC transcriptome
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Protein 4.1R affects photodynamic therapy for B16 melanoma by regulating the transport of 5-aminolevulinic acid Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-29 Bowen Li; Xiaolin Zhang; Yu Lu; Luyang Zhao; Yaxin Guo; Shuangshuang Guo; Qiaozhen Kang; Jingjing Liu; Liping Dai; Liguo Zhang; Dandan Fan; Zhenyu Ji
Melanoma is the most aggressive malignant tumor of skin cancer as it can grow rapidly and metastasize. Photodynamic therapy (PDT) is a promising cancer ablation method for skin tumors, although it lacks efficiency owing to factors such as tumor characteristics, delivery of photosensitizers, immune response in vivo etc. Extensive investigation of molecules that can potentially modulate treatment efficacy
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Lymphocytic microparticles suppress retinal angiogenesis via targeting Müller cells in the ischemic retinopathy mouse model Exp. Cell Res. (IF 3.383) Pub Date : 2021-01-09 ChenRongRong Cai; Houda Tahiri; Carl Fortin; Christina Ortiz; Helene Sintjago; Chun Yang; Pierre Hardy
Retinopathy of prematurity (ROP) is the primary cause of visual impairment and vision loss in premature infants, which results from the formation of aberrant retinal neovascularization (NV). An emerging body of evidence has shown that Müller cells are the predominant source of vascular endothelial growth factor (VEGF), which also serves as a chemoattractant for monocyte/macrophage lineage. The recruitment
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Identification of protein/mRNA network involving the PSORS1 locus gene CCHCR1 and the PSORS4 locus gene HAX1 Exp. Cell Res. (IF 3.383) Pub Date : 2021-01-05 Cinzia Pisani; Annalisa Onori; Francesca Gabanella; Maria Grazia Di Certo; Claudio Passananti; Nicoletta Corbi
CCHCR1 (Coiled-Coil alpha-Helical Rod 1), maps to chromosomal region 6p21.3, within the major psoriasis susceptibility locus PSORS1. CCHCR1 itself is a plausible psoriasis candidate gene, however its role in psoriasis pathogenesis remains unclear. We previously demonstrated that CCHCR1 protein acts as a cytoplasmic docking site for RNA polymerase II core subunit 3 (RPB3) in cycling cells, suggesting
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Continuous NF-κB pathway inhibition promotes expansion of human phenotypical hematopoietic stem/progenitor cells through metabolism regulation Exp. Cell Res. (IF 3.383) Pub Date : 2021-01-08 Qihao Sun; Yan Fu; Xuejun Zhu; Wen-Song Tan; Haibo Cai
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Marf-mediated mitochondrial fusion is imperative for the development and functioning of indirect flight muscles (IFMs) in Drosophila Exp. Cell Res. (IF 3.383) Pub Date : 2021-01-13 Prasanna Katti; Mamta Rai; Shubhi Srivastava; Patrick D’ Silva; Upendra Nongthomba
Dynamic changes in mitochondrial shape and size are vital for mitochondrial health and for tissue development and function. Adult Drosophila indirect flight muscles contain densely packed mitochondria. We show here that mitochondrial fusion is critical during early muscle development (in pupa) and that silencing of the outer mitochondrial membrane fusion gene, Marf, in muscles results in smaller mitochondria
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Lipopolysaccharide and lipoteichoic acid influence milk production ability via different early responses in bovine mammary epithelial cells Exp. Cell Res. (IF 3.383) Pub Date : 2021-01-12 Yusaku Tsugami; Haruka Wakasa; Manabu Kawahara; Takanori Nishimura; Ken Kobayashi
Lipopolysaccharide (LPS) and lipoteichoic acid (LTA) are cell wall components of Escherichia coli and Staphylococcus aureus, which cause clinical and subclinical mastitis, respectively. However, the reason of the difference in symptoms by pathogen type remains unclear. In this study, the influence of LPS and LTA on early response and milk production in lactating bovine mammary epithelial cells (BMECs)
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Sixty days of head-down tilt bed rest with or without artificial gravity do not affect the neuromuscular secretome Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-29 Bergita Ganse; Alessandra Bosutti; Michael Drey; Hans Degens
Artificial gravity is a potential countermeasure to attenuate effects of weightlessness during long-term spaceflight, including losses of muscle mass and function, possibly to some extent attributable to disturbed neuromuscular interaction. The 60-day AGBRESA bed-rest study was conducted with 24 participants (16 men, 8 women; 33 ± 9 years; 175 ± 9 cm; 74 ± 10 kg; 8 control group, 8 continuous (cAG)
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Kindlin-3 mutation in mesenchymal stem cells results in enhanced chondrogenesis Exp. Cell Res. (IF 3.383) Pub Date : 2021-01-05 Bethany A. Kerr; Lihong Shi; Alexander H. Jinnah; Koran S. Harris; Jeffrey S. Willey; Donald P. Lennon; Arnold I. Caplan; Tatiana V. Byzova
Identifying patient mutations driving skeletal development disorders has driven our understanding of bone development. Integrin adhesion deficiency disease is caused by a Kindlin-3 (fermitin family member 3) mutation, and its inactivation results in bleeding disorders and osteopenia. In this study, we uncover a role for Kindlin-3 in the differentiation of bone marrow mesenchymal stem cells (BMSCs)
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AFF4 facilitates melanoma cell progression by regulating c-Jun activity Exp. Cell Res. (IF 3.383) Pub Date : 2021-01-05 Hongyan Hu; Yi Zhang; Liufang Zhao; Wentao Zhao; Xiaoxiong Wang; En Ye; Yan Dong; Lijuan Zhang; Fengming Ran; Yongchun Zhou; Yunchao Huang
Melanoma is characterized by high mortality and poor prognosis due to metastasis. AFF4 (AF4/FMR2 family member 4), as a scaffold protein, is a component of the super elongation complex (SEC), and is involved in the progression of tumors, e.g., leukemia, head and neck squamous cell carcinoma (HNSCC). However, few studies on AFF4 have focused on melanoma. Here, AFF4 expression levels and clinicopathological
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Src-mediated Tyr353 phosphorylation of IP3R1 promotes its stability and causes apoptosis in palmitic acid-treated hepatocytes Exp. Cell Res. (IF 3.383) Pub Date : 2021-01-05 Ting Yu; Enze Zheng; Yanping Li; Yuqi Li; Jun Xia; Qiuying Ding; Zhengping Hou; Xiong Z. Ruan; Lei Zhao; Yaxi Chen
Palmitic acid (PA)-induced hepatocyte apoptosis is critical for the progression of nonalcoholic fatty liver disease (NAFLD). Inositol 1,4,5-trisphosphate receptor type 1 (IP3R1) is an intracellular Ca2+-release channel and is involved in PA-induced hepatocyte apoptosis. While the expression of IP3R1 is elevated in patients with NAFLD and in hepatocytes treated with PA, it remains unclear how PA promotes
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MiR-21-3p triggers cardiac fibroblasts pyroptosis in diabetic cardiac fibrosis via inhibiting androgen receptor Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-29 Peng Shi; Xu-Dong Zhao; Kai-Hu Shi; Xuan-Sheng Ding; Hui Tao
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SIRT1 reduces epigenetic and non-epigenetic changes to maintain the quality of postovulatory aged oocytes in mice Exp. Cell Res. (IF 3.383) Pub Date : 2021-01-05 Xupeng Xing; Jinjing Zhang; Teng Wu; Jingcheng Zhang; Yongsheng Wang; Jianmin Su; Yong Zhang
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Synaptonemal complex formation produces a particular arrangement of the lateral element-associated DNA Exp. Cell Res. (IF 3.383) Pub Date : 2021-01-02 Rosario Ortiz; Silvia Juárez Chavero; Olga M. Echeverría; Abrahan Hernandez-Hernandez
During meiosis, homologous chromosomes exchange genetic material. This exchange or meiotic recombination is mediated by a proteinaceous scaffold known as the Synaptonemal complex (SC). Any defects in its formation produce failures in meiotic recombination, chromosome segregation and meiosis completion. It has been proposed that DNA repair events that will be resolved by crossover between homologous
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A role for Rab30 in retrograde trafficking and maintenance of endosome-TGN organization Exp. Cell Res. (IF 3.383) Pub Date : 2021-01-05 Khalisah L. Zulkefli; Ismail S. Mahmoud; Nicholas A. Williamson; Prajakta Kulkarni Gosavi; Fiona J. Houghton; Paul A. Gleeson
Rab30 is a poorly characterized small GTPase. Here we show that Rab30 is localized primarily to the TGN and recycling endosomes in a range of cell types, including primary neurons; minor levels of Rab30 were also detected throughout the Golgi stack and early endosomes. Silencing of Rab30 resulted in the dispersal of both early and recycling endosomes and TGN compartments in HeLa cells. By analyzing
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Novel method for effectively amplifying human peripheral blood T cells in vitro Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-19 Jun Ye; Wanwan Cao; Zhibo Tao; Shuping Zhao; Chunling Wang; Xing Xu; Ai Zhao; Jimin Gao
The use of chimeric antigen receptor-modified T cells (CAR T cells) is an effective therapy for advanced cancer, especially hematological malignancies, and this method has attracted widespread attention in the last several years. The type, number and vitality of the effector cells clearly play important roles in this approach. In this study, to expand the possibility of curing cancer through adoptive
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High expression of Notch2 drives tongue squamous cell carcinoma carcinogenesis Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-28 Rui-huan Gan; Li-song Lin; Dan-ping Zheng; Yong Zhao; Lin-can Ding; Da-li Zheng; You-guang Lu
Tongue squamous cell carcinoma (TSCC) is one of the most common cancers in the oral cavity. Notch signaling is frequently dysregulated in cancer. However, the role of Notch2 in TSCC is not well understood. The aim of this study was to investigate the effect of abnormal expression of Notch2 in TSCC. The expression of Notch2 was tested in 47 pairs of tissues from tongue cancer and normal samples by using
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TRIM27-mediated ubiquitination of PPARγ promotes glutamate-induced cell apoptosis and inflammation Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-30 Yong Hao; Shuwei Bai; Jing Peng; Ronghua Hong; Jie Ding; Zezhi Li; Yangtai Guan
Neurotoxicity induced by glutamate (Glu) is often used to study the signaling mechanism of neurological disorders. The identification of specific genetic factors that cause Glu-induced neurotoxicity provides evidence for the common pathways of neuronal apoptosis and inflammation. TRIM27 has been found to induce apoptosis and inflammation. Nevertheless, there is little evidence that TRIM27 is associated
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RNA binding protein DAZAP1 promotes HCC progression and regulates ferroptosis by interacting with SLC7A11 mRNA Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-29 Qi Wang; Yaxun Guo; Wentao Wang; Bingqi Liu; Guangsheng Yang; Zongzhen Xu; Jie Li; Zhiqian Liu
RNA-binding proteins (RBPs) closely regulate the whole lifecycle of most RNA molecules, from the very early stage of transcription to RNA decay. Dysregulation of RBPs significantly affects the fate of cancer-related transcripts. Therefore, it is imperative to fully understand the complicated RBP-RNA regulatory networks in malignant diseases and to explore novel therapeutic targets. The RBP DAZAP1 (deleted
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Heat shock induces the nuclear accumulation of YAP1 via SRC Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-22 Xinliang Jiang; Junichi Maruyama; Hiroaki Iwasa; Kyoko Arimoto-Matsuzaki; Hiroshi Nishina; Yutaka Hata
Yes-associated protein 1 (YAP1), a co-transcription activator, shuttles between the cytoplasm and the nucleus. Phosphorylation by large tumor suppressor kinases (LATS1/2) is the major determinant of YAP1 subcellular localization. Unphosphorylated YAP1 interacts with transcription factors in the nucleus and regulates gene transcription, while phosphorylated YAP1 is trapped in the cytoplasm and is degraded
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EGLP-1 lowers body weight better than exendin-4 by reducing food intake and increasing basal energy expenditure in diet-induced obese mice Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-25 Huashan Gao; Qian Zhao; Kaiying Li; Fujian Qin; Xin Yin; Zhou Lu; Ziwei Song; You Wu; Yumeng Shen; Yi Pan; Liang Jin; Yanfeng zhang
It is well known that GLP-1 activates GLP-1R to reduce body weight by inhibiting eating. GLP-1 is cleaved by the neutral endopeptidase (NEP) 24.11 into a pentapeptide GLP-1 (32–36) amide, which increases basal energy expenditure and inhibits weight gain in obese mice. It is well known that GLP-1 analogs can reduce weight by suppressing eating. However, there are few reports of reducing weight through
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Surface distribution of heterogenous clathrin assemblies in resorbing osteoclasts Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-23 Toshitaka Akisaka; Atsushi Yoshida
Osteoclasts seeded on either glass coverslips or apatite pellets have at least two morphologically distinct substrate adhesion sites: actin-based adhesion structures including podosome belts and sealing zones, and adjacent clathrin sheets. Clathrin-coated structures are exclusively localized at the podosome belts and sealing zone, in both of which the plasma membrane forms a tight attachment to the
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Hypoxia-cultured mouse mesenchymal stromal cells from bone marrow and compact bone display different phenotypic traits Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-16 Rafaela Rossetti; Felipe Augusto Rós; Lucas Eduardo Botelho de Souza; Juliana de Matos Maçonetto; Péricles Natan Mendes da Costa; Fernanda Ursoli Ferreira; Josiane Serrano Borges; Julianne Vargas de Carvalho; Nayara Patrícia Morotti; Simone Kashima; Dimas Tadeu Covas
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U2AF - Hypoxia-induced fas alternative splicing regulator Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-26 Laurynas Vilys; Inga Peciuliene; Egle Jakubauskiene; Ruta Zinkeviciute; Yuichi Makino; Arvydas Kanopka
The splicing machinery heavily contributes to biological complexity and especially to the ability of cells to adapt to altered cellular conditions. Hypoxia also plays a key role in the pathophysiology of many disease states. Recent studies have revealed that tumorigenesis and hypoxia are involved in large-scale alterations in alternative pre-mRNA splicing. Fas pre-mRNA is alternatively spliced by excluding
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Exosomes secreted from mesenchymal stem cells mediate the regeneration of endothelial cells treated with rapamycin by delivering pro-angiogenic microRNAs Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-19 Weizong Wang; Yixin Zhao; Huilin Li; Yujiao Zhang; Xiaomeng Jia; Cong Wang; Pengju Zhu; Jiangrong Wang; Yinglong Hou
Delayed endothelial healing after drug eluting stent (DES) implantation is a critical clinical problem in treatment of coronary artery diseases. Exosomes exhibit proangiogenic potential in a variety of ischemic diseases. However, the association of exosomes with endothelial regeneration after DES implantation has been rarely reported. In this study, we aimed to investigate the therapeutic effects of
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Knockdown of DDX46 suppresses the proliferation and invasion of gastric cancer through inactivating Akt/GSK-3β/β-catenin pathway Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-19 Lihong Chen; Min Xu; Wenting Zhong; Yinghui Hu; Guanghui Wang
DEAD-box RNA helicase 46 (DDX46) has recently been identified as a candidate oncogene in several types of human malignancies. To date, the role of DDX46 in gastric cancer has not been determined. The purpose of the current study was to explore the role of DDX46 in gastric cancer and the potential mechanism. DDX46-silecing or overexpressing gastric cancer cell lines were established to validate the
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Adaptation of metabolism to multicellular aggregation, hypoxia and obese stromal cell incorporation as potential measure of survival of ovarian metastases Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-15 Stephanie L.E. Compton; Emily S. Pyne; Lu Liu; Jack Guinan; Amanda A. Shea; Joseph P. Grieco; Madlyn I. Frisard; Eva M. Schmelz
Ovarian metastases exfoliate from the primary tumor and it is thought that aggregation supports their survival in the peritoneal cavity during dissemination but the underlying mechanisms are not clearly identified. We have previously shown that ovarian cancer cells acquire an increasingly glycolytic and metabolic flexible phenotype during progression. In the present study, we investigated how hypoxia
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Vascular endothelial and smooth muscle cell galvanotactic response and differential migratory behavior Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-18 Kaitlyn R. Ammann; Marvin J. Slepian
Chronic disease or injury of the vasculature impairs the functionality of vascular wall cells particularly in their ability to migrate and repair vascular surfaces. Under pathologic conditions, vascular endothelial cells (ECs) lose their non-thrombogenic properties and decrease their motility. Alternatively, vascular smooth muscle cells (SMCs) may increase motility and proliferation, leading to blood
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Long non-coding RNA GClnc1 knockdown suppresses progression of epithelial ovarian cancer by recruiting FOXC2 to disrupt the NOTCH1/NF-κB/Snail pathway Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-16 Dandan Wu; Yumin Ke; Rongrong Xiao; Jia Liu; Qingli Li; Yiwen Wang
Purpose Epithelial ovarian cancer (EOC) is a highly fatal gynecological cancer. A long noncoding RNA (lncRNA) gastric cancer-associated lncRNA1 (GClnc1) has been revealed to play critical roles in metastasis. Therefore, the present study aims to explore the correlation between GClnc1 and the metastasis and progression of EOC. Methods First, 57 paired EOC and paracancerous tissues were collected to
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Antineoplastic activity of Salmonella Typhimurium outer membrane nanovesicles Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-15 Rasha GO. Aly; Mona IH. El-Enbaawy; Sahar S Abd El-Rahman; Nagwa S. Ata
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Endothelium-specific endothelin-1 expression promotes pro-inflammatory macrophage activation by regulating miR-33/NR4A axis Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-16 Juan Zhang; Wen-shu Zhao; Lin Xu; Xin Wang; Xiao-li Li; Xin-chun Yang
The hallmark of atherogenesis is characterized as endothelial dysfunction and subsequent macrophage activation. Although our previous study has demonstrated that endothelin-1 (ET-1) plays an important role in atherogenesis, the underlying mechanism remains deeply investigation. Enhanced atherosclerotic plaques were observed in endothelium-specific ET-1 overexpression ApoE−/- mice (eET-1/ApoE−/-) concomitant
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Effects of laminin-111 peptide coatings on rat neural stem/progenitor cell culture Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-22 Hideki Hayashi; Ichiro Horinokita; Yuji Yamada; Keisuke Hamada; Norio Takagi; Motoyoshi Nomizu
Neurons require adhesive scaffolds for their growth and differentiation. Laminins are a major cell adhesive component of basement membranes and have various biological activities in the peripheral and central nervous systems. Here, we evaluated the biological activities of 5 peptides derived from laminin-111 as a scaffold for mouse neuroblastoma Neuro2a cells and rat neural stem/progenitor cells (NPCs)
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HPRT promotes proliferation and metastasis in head and neck squamous cell carcinoma through direct interaction with STAT3 Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-21 Lin Wang; Yupu Wang; Nannan Han; Xu Wang; Min Ruan
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LncRNA embryonic stem cells expressed 1 (Lncenc1) is identified as a novel regulator in neuropathic pain by interacting with EZH2 and downregulating the expression of Bai1 in mouse microglia Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-16 Zhongyi Zhang; Xiufang Sun; Guoquan Zhao; Yingcun Ma; Guoli Zeng
LncRNA embryonic stem cells expressed 1 (Lncenc1), named after its high expression in naïve embryonic stem cells (nESCs), has been rarely studied in almost all pathological processes. Evidences suggest that Lncenc1 is likely to work in the form of RNA-protein complex. Here, we found that Lncenc1 in dorsal root ganglion (DRG) was significantly upregulated in response to mouse nerve injury caused by
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Revealing functional insights into ER proteostasis through proteomics and interactomics Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-08 Madison T. Wright; Lars Plate
The endoplasmic reticulum (ER), responsible for processing approximately one-third of the human proteome including most secreted and membrane proteins, plays a pivotal role in protein homeostasis (proteostasis). Dysregulation of ER proteostasis has been implicated in a number of disease states. As such, continued efforts are directed at elucidating mechanisms of ER protein quality control which are
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Rictor-targeting exosomal microRNA-16 ameliorates lung fibrosis by inhibiting the mTORC2-SPARC axis Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-09 Minoru Inomata; Koichiro Kamio; Arata Azuma; Kuniko Matsuda; Jiro Usuki; Akemi Morinaga; Toru Tanaka; Takeru Kashiwada; Kenichiro Atsumi; Hiroki Hayashi; Kazue Fujita; Yoshinobu Saito; Kaoru Kubota; Masahiro Seike; Akihiko Gemma
Idiopathic pulmonary fibrosis (IPF), a progressive disorder of unknown etiology, is characterized by pathological lung fibroblast activation and proliferation resulting in abnormal deposition of extracellular matrix proteins within the lung parenchyma. The pathophysiological roles of exosomal microRNAs in pulmonary fibrosis remain unclear; therefore, we aimed to identify and characterize fibrosis-responsive
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Maternal Undernutrition Modulates Hepatic MicroRNAs Expression in the Early Life of Offspring Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-19 Weifen Zhu; Weiwei Gui; Xihua Lin; Xueyao Yin; Li Liang; Hong Li
Emerging studies revealed that a poor intrauterine environment elicited by maternal nutrient restriction (MNR) is associated with an increased risk of metabolic diseases in adulthood. Previous research has shown that microRNAs (miRNAs) exert pivotal roles in modulating molecular pathways involved in disease pathogenesis and progression. In this respect, we herein examined miRNA profiles in samples
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Establishment of FXS-A9 panel with a single human X chromosome from fragile X syndrome-associated individual Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-06 Yuji Nakayama; Kaori Adachi; Nofirifumi Shioda; Shoya Maeta; Eiji Nanba; Hiroyuki Kugoh
Fragile X syndrome (FXS) is the most common inheritable form of intellectual disability. FMR1, the gene responsible for FXS, is located on human chromosome Xq27.3 and contains a stretch of CGG trinucleotide repeats in its 5′ untranslated region. FXS is caused by CGG repeats that expand beyond 200, resulting in FMR1 silencing via promoter hypermethylation. The molecular mechanism underlying CGG repeat
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Activation of the CaR-CSE/H2S pathway confers cardioprotection against ischemia-reperfusion injury Exp. Cell Res. (IF 3.383) Pub Date : 2020-11-19 Ying Luo; Li-Mei Liu; Li Xie; Hong-Lei Zhao; Yong-Kang Lu; Bao-Quan Wu; Zhi-Ye Wu; Zhi-Ling Zhang; Yun-Ling Hao; Wu-Hua Ou; Rui-Shuang Liu; Wen-Min Xu; Xie-Hui Chen
Ischemia-reperfusion (I/R) injury is a multifactorial process triggered when an organ is subjected to transiently reduced blood supply. The result is a cascade of pathological complications and organ damage due to the production of reactive oxygen species following reperfusion. The present study aims to evaluate the role of activated calcium-sensing receptor (CaR)-cystathionine γ-lyase (CSE)/hydrogen
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HIF1α promotes tumor chemoresistance via recruiting GDF15-producing TAMs in colorectal cancer Exp. Cell Res. (IF 3.383) Pub Date : 2020-11-23 Hongtu Zheng; Shan Yu; Congcong Zhu; Tianan Guo; Fangqi Liu; Ye Xu
Chemoresistance is a tremendous challenge to efficacy of systemic chemotherapy which is the preferred treatment for the advanced CRC patients. More tumor-associated macrophages (TAMs) are recruited into the CRC tumor under chemotherapy, which are highly implicated in the chemoresistance development, but the underlying molecular mechanism is unclear. Here, we present that activated HIF1α signaling in
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Dl-3-n-butylphthalide promotes neurite outgrowth of primary cortical neurons by Sonic Hedgehog signaling via upregulating Gap43 Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-06 Cong Zhang; Lili Cui; Weiliang He; Xiangjian Zhang; Huaijun Liu
Neurite outgrowth is the basis for wiring during the development of the nervous system. Dl-3-n-butylphthalide (NBP) has been recognized as a promising treatment to improve behavioral, neurological and cognitive outcomes in ischemic stroke. However, little is known about the effect and mechanism of NBP on the neurite outgrowth. In this study, we used different methods to investigate the potential effects
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Oestrogen regulates SOX9 bioavailability by rapidly activating ERK1/2 and stabilising microtubules in a human testis-derived cell line Exp. Cell Res. (IF 3.383) Pub Date : 2020-11-30 Melanie K. Stewart; Deidre M. Mattiske; Andrew J. Pask
Nuclear SOX9 is essential for Sertoli cell differentiation and the development of a testis. Exposure of Sertoli cells to exogenous oestrogen causes cytoplasmic retention of SOX9, inhibiting testis development and promoting ovarian development. The cytoplasmic localisation of SOX9 requires a stabilised microtubule network and a key MAPK complex, ERK1/2, is responsive to oestrogen and known to affect
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NIMA-related kinase 7 amplifies NLRP3 inflammasome pro-inflammatory signaling in microglia/macrophages and mice models of spinal cord injury Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-09 Xubiao Ji; Zhiwen Song; Junsheng He; Shiwu Guo; Yuwei Chen; Honghai Wang; Jinlong Zhang; Xu Xu; Jinbo Liu
Background NIMA-related kinase-7 (NEK7) is a serine/threonine kinase that drives cell-cycle dynamics by modulating mitotic spindle formation and cytokinesis. It is also a crucial modulator of the pro-inflammatory effects of NOD-like receptor 3 (NLRP3) inflammasome. However, the role of NEK7 in microglia/macrophages post-spinal cord injury (SCI) is not well defined. Methods In this study, we performed
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Endocytosis of the non-catalytic ADAM23: Recycling and long half-life properties Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-06 Ingrid L.M. Souza; Natália H. Oliveira; Pierina A.M. Huamaní; Anh-Tuan S. Martin; Zaine L.M. Borgonovo; Lia S. Nakao; Silvio M. Zanata
A Disintegrin And Metalloprotease 23 (ADAM23) is a member of the ADAMs family of transmembrane proteins, mostly expressed in nervous system, and involved in traffic and stabilization of Kv1-potassium channels, synaptic transmission, neurite outgrowth, neuronal morphology and cell adhesion. Also, ADAM23 has been linked to human pathological conditions, such as epilepsy, cancer metastasis and cardiomyopathy
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Cancer/testis antigen LDHC promotes proliferation and metastasis by activating the PI3K/Akt/GSK-3β-signaling pathway and the in lung adenocarcinoma Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-08 Liangyuan Chen; Qiumei Wu; Xunyu Xu; Caihong Yang; Jianbin You; Falin Chen; Yanfen Zeng
The cancer/testis antigen lactate dehydrogenase-C4 (LDHC) is a specific isoenzyme of the LDH family that regulates invasion and metastasis in some malignancies; however, little is known regarding its role in progression of lung adenocarcinoma (LUAD). Thus, we investigated LDHC expression by immunohistochemistry, and analyzed its clinical significance in 88 LUAD specimens. The role and molecular mechanisms
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Protein 4.1R negatively regulates P815 cells proliferation by inhibiting C-Kit-mediated signal transduction Exp. Cell Res. (IF 3.383) Pub Date : 2020-11-30 Cong Ding; Yuying Guo; Taotao Liang; Jiaojiao Liu; Lu Yang; Ting Wang; Xin Liu; Qiaozhen Kang
The proliferation of mast cells (MCs) plays a crucial role in either physiological or pathological progression of human physical. C-Kit-mediated signaling pathway has been confirmed to play a key role in MCs proliferation, and the regulatory mechanisms of C-Kit-mediated MCs proliferation need to be further explored. Our previous study found that protein 4.1R could negatively regulate T cell receptor
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Ubiquitin-specific protease 14 promotes radio-resistance and suppresses autophagy in oral squamous cell carcinoma Exp. Cell Res. (IF 3.383) Pub Date : 2020-11-16 Weihong Xie; Lijuan Xu
Oral squamous cell carcinoma (OSCC) is a common malignant tumor in the world. Radiotherapy is one of the standard therapies for patients with OSCC, but its clinical efficiency is limited due to radioresistance. In this study, we identified a mechanism of such resistance regulated by Ubiquitin-specific protease 14 (USP14). USP14 expression was significantly increased in clinical OSCC tissue samples
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Combination therapy with lenvatinib and radiation significantly inhibits thyroid cancer growth by uptake of tyrosine kinase inhibitor Exp. Cell Res. (IF 3.383) Pub Date : 2020-11-21 Kensuke Suzuki; Hiroshi Iwai; Keita Utsunomiya; Yumiko Kono; Yoshiki Kobayashi; Dan Van Bui; Shunsuke Sawada; Yasutaka Yun; Akitoshi Mitani; Naoyuki Kondo; Tayo Katano; Noboru Tanigawa; Tomoya Akama; Akira Kanda
Although surgical treatment cures >90% of differentiated thyroid cancer (DTC) patients, the remaining patients, including advanced DTC cases, have poor clinical outcomes. These patients with inoperable disease have only two choices of radioactive iodine therapy and tyrosine kinase inhibitors such as lenvatinib, which have a high incidence of treatment-related adverse events and can only prolong progression
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Exosomes derived from thymic stromal lymphopoietin-treated dendritic cells regulate T helper 17/regulatory T cell differentiation via miR-21/Smad7 axis Exp. Cell Res. (IF 3.383) Pub Date : 2020-11-28 Qingshan Ji; Jiajia Liu; Yiran Dong; Lisong Wang; Kai Dong; Berthold Setiz; Nóra Szentmáry; Bin Qu; Lei Shi
Thymic stromal lymphopoietin (TSLP) is associated with fungal keratitis. This work aims to investigate whether TSLP can regulate T helper (Th) 17 and regulatory T cell (Treg) differentiation. We separated dendritic cells (DCs) from peripheral blood of healthy volunteers. DCs were treated with TSLP to activate DCs, and exosomes were obtained. CD+ T cells were incubated with exosomes from TSLP-treated
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Loss of MBD2 affects early T cell development by inhibiting the WNT signaling pathway Exp. Cell Res. (IF 3.383) Pub Date : 2020-11-30 Ling Cheng; Kuangguo Zhou; Xing Chen; Jing Zhou; Wei Cai; Yingchi Zhang; Xiaomin Wang; Congyi Wang; Weiping Yuan; Jianfeng Zhou; Mi Zhou
DNA methylation alters the expression of certain genes without any alteration to the DNA sequence and is a dynamic process during normal hematopoietic differentiation. As an epigenetic regulator, methyl-CpG-binding domain protein 2 (MBD2) is an important member of the MBD protein family and is acknowledged as a “reader” of DNA methylation. We used a mouse model to study the effects of MBD2 on the early
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The role of vascular niche and endothelial cells in organogenesis and regeneration Exp. Cell Res. (IF 3.383) Pub Date : 2020-11-30 Domenico Ribatti; Roberto Tamma; Tiziana Annese
The term vascular niche indicate the physical and biochemical microenvironment around blood vessel where endothelial cells, pericytes, and smooth muscle cells organize themselves to form blood vessels and release molecules involved in the recruitment of hematopoietic stem cells, endothelial progenitor cells and mesenchymal stem cells. The vascular niche creates a permissive environment that enables
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Cold shock induces novel nuclear bodies in Xenopus oocytes Exp. Cell Res. (IF 3.383) Pub Date : 2020-11-18 Ji-Long Liu; Joseph G. Gall
Here we describe novel spherical structures that are induced by cold shock on the lampbrush chromosomes (LBCs) of Xenopus laevis oocytes. We call these structures cold bodies or C-bodies. C-bodies are distributed symmetrically on homologous LBCs, with a pattern similar to that of 5S rDNA. Neither active transcription nor translation is necessary for their formation. Similar protrusions occur on the
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Simultaneous gentamicin-mediated damage and Atoh1 overexpression promotes hair cell regeneration in the neonatal mouse utricle Exp. Cell Res. (IF 3.383) Pub Date : 2020-12-03 Xiaoqing Qian; Rui Ma; Xinwei Wang; Xinda Xu; Juanmei Yang; Fanglu Chi; Dongdong Ren
Loss of hair cells from vestibular epithelium results in balance dysfunction. The current therapeutic regimen for vestibular diseases is limited. Upon injury or Atoh1 overexpression, hair cell replacement occurs rapidly in the mammalian utricle, suggesting a promising approach to induce vestibular hair cell regeneration. In this study, we applied simultaneous gentamicin-mediated hair cell ablation
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