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Assessment of quadrivalent characteristics influencing chromosome segregation by analyzing human preimplantation embryos from reciprocal translocation carriers Comp. Cytogenet. (IF 1.0) Pub Date : 2024-01-22 Ziravard N. Tonyan, Irina L. Puppo, Alsu F. Saifitdinova, Tatyana V. Vavilova, Andrey S. Glotov
Patterns of meiotic chromosome segregation were analyzed in cleavage stage and blastocyst stage human embryos from couples with autosomal reciprocal translocations (ART). The influence of quadrivalent asymmetry degree, the presence of terminal breakpoints, and the involvement of acrocentric chromosomes in the rearrangement were analyzed to evaluate their contribution to the formation of non-viable
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Chromosomes of the genus Arge Schrank, 1802 (Hymenoptera, Argidae): new data and review Comp. Cytogenet. (IF 1.0) Pub Date : 2023-12-21 Vladimir E. Gokhman
Results of the chromosome study of 12 sawfly species of the genus Arge Schrank, 1802 are reviewed, including new data on the karyotypes of A. ciliaris (Linnaeus, 1767) and A. enodis (Linnaeus, 1767) with n = 10. Moreover, the same chromosome number, n = 10, is found in A. ustulata (Linnaeus, 1758), for which n = 8 was previously reported. In addition, n = 8 is confirmed in A. gracilicornis (Klug
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An updated Atlas of Helophorus chromosomes Comp. Cytogenet. (IF 1.0) Pub Date : 2023-12-21 Robert B. Angus
An account is given of my development of techniques to obtain well-spread Giemsa-stained banded chromosome preparations. Apparent G-banding could be obtained following very slight trypsin treatment of freshly prepared slides, but this banding was very fine (close-grained) and possibly not a reflection of chromosome structure. However, treatment of developing embryos in vitro with 5-fluorouridine
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Karyotype diversity in the genus Nysius Dallas, 1852 (Hemiptera, Heteroptera, Lygaeidae) is much greater than you might think Comp. Cytogenet. (IF 1.0) Pub Date : 2023-12-18 Natalia V. Golub, Boris A. Anokhin, Valentina G. Kuznetsova
We studied the karyotype and chromosomal distribution of 18S rDNA clustered in nucleolar organizer regions (NORs) in Nysius graminicola (Kolenati, 1845), belonging to the subfamily Orsillinae (Lygaeidae). It is shown that this species has a karyotype with 2n = 22(18+mm+XY), previously known in only one of 24 studied species of the genus Nysius Dallas, 1852, characterized by a similar karyotype, 2n
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Karyotype and reproductive traits of the unique symbiotic mealybug Orbuspedum machinator G.-Z. (Homoptera, Coccinea) Comp. Cytogenet. (IF 1.0) Pub Date : 2023-12-18 Ilya A. Gavrilov-Zimin
The karyotype and reproductive features of Orbuspedum machinator Gavrilov-Zimin, 2017 (Pseudococcidae) were studied for the first time. Diploid chromosome number is 18 in females. Reproduction is probably bisexual, as indicated by the presence of characteristic Lecanoid heterochromatinization of the paternal set of chromosomes in embryonic cells of about 50% of the embryos studied. The female reproductive
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Metaphase chromosomes of five Neotropical species of the genus Drosophila (Diptera, Drosophilidae) Comp. Cytogenet. (IF 1.0) Pub Date : 2023-12-05 Doris Vela, Erika Villavicencio
The mitotic metaphases of five Andean species of genus Drosophila are described for the first time. The evolutionary and interspecific genetic relationships within three Neotropical Drosophila species groups are analyzed. The diploid chromosome number for each species is as follows: D. cashapamba Céspedes et Rafael, 2012 2n = 6 (2V, 1J) (X = J, Y = R), D. ecuatoriana Vela et Rafael, 2004 2n = 10
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Cell culture and karyotypic description of Pseudophryne coriacea (Keferstein, 1868) (Amphibia, Anura) from the New South Wales Central Coast Comp. Cytogenet. (IF 1.0) Pub Date : 2023-11-10 Richard Mollard, Michael Mahony
The karyotype of the IUCN least concern red-backed toadlet Pseudophryne (P.) coriacea (Keferstein, 1868) from the New South Wales Central Coast is described following tissue culture of toe clipping macerates and conventional DAPI staining. The diploid number is 2n = 24. The karyotype is represented by six large and five small chromosomal pairs and one very small chromosomal pair. The very small chromosome
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Divergent karyotypes in five genera of the African endemic fish family Distichodontidae (Cithariniformes, Osteichthyes) Comp. Cytogenet. (IF 1.0) Pub Date : 2023-11-02 Sergey A. Simanovsky, Dmitry A. Medvedev, Fekadu Tefera, Alexander S. Golubtsov
The African family Distichodontidae comprises 109 species in 16 genera. Up-to-date cytogenetic information was available for the only distichodontid species Distichodus affinis Günther, 1873. Here we report chromosome number and morphology in: Distichodus engycephalus Günther, 1864 (2n = 52, FN = 104), Ichthyborus besse (Joannis, 1835) (2n = 46, FN = 92), Nannocharax niloticus (Joannis, 1835) (2n
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Chromosome study of the Hymenoptera (Insecta): from cytogenetics to cytogenomics Comp. Cytogenet. (IF 1.0) Pub Date : 2023-11-01 Vladimir E. Gokhman
A brief overview of the current stage of the chromosome study of the insect order Hymenoptera is given. It is demonstrated that, in addition to routine staining and other traditional techniques of chromosome research, karyotypes of an increasing number of hymenopterans are being studied using molecular methods, e.g., staining with base-specific fluorochromes and fluorescence in situ hybridization
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Ancient reproductive modes and criteria of multicellularity Comp. Cytogenet. (IF 1.0) Pub Date : 2023-10-20 Ilya A. Gavrilov-Zimin
It is demonstrated that the initial method of fertilization in animals (Metazoa), embryophyte plants (Embryophyta), most groups of multicellular oogamous algae, oogamous and pseudoogamous multicellular fungi was internal fertilization (in the broad meaning) in/on the body of a maternal organism. Accordingly, during the bisexual process, the initial method of formation of a daughter multicellular
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A comparative cytogenetic study of Hypsibarbus malcolmi and H. wetmorei (Cyprinidae, Poropuntiini) Comp. Cytogenet. (IF 1.0) Pub Date : 2023-09-15 Sudarat Khensuwan, Weerayuth Supiwong, Chatmongkon Suwannapoom, Phichaya Buasriyot, Sitthisak Jantarat, Weera Thongnetr, Nawarat Muanglen, Puntivar Kaewmad, Pasakorn Saenjundaeng, Kriengkrai Seetapan, Thomas Liehr, Alongklod Tanomtong
Cyprininae are a highly diversified but demonstrably monophyletic lineage of cypriniform fishes. Here, the karyotype and chromosomal characteristics of Hypsibarbus malcolmi (Smith, 1945) and H. wetmorei (Smith, 1931) were examined using conventional, nucleolus organizing regions (NORs) and molecular cytogenetic protocols. The diploid chromosome number (2n) of H. malcolmi was 50, the fundamental number
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Complete chloroplast genome sequence of Rhododendron mariesii and comparative genomics of related species in the family Ericaeae Comp. Cytogenet. (IF 1.0) Pub Date : 2023-08-18 Zhiliang Li, Zhiwei Huang, Xuchun Wan, Jiaojun Yu, Hongjin Dong, Jialiang Zhang, Chunyu Zhang, Shuzhen Wang
Rhododendron mariesii Hemsley et Wilson, 1907, a typical member of the family Ericaeae, possesses valuable medicinal and horticultural properties. In this research, the complete chloroplast (cp) genome of R. mariesii was sequenced and assembled, which proved to be a typical quadripartite structure with the length of 203,480 bp. In particular, the lengths of the large single copy region (LSC), small
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Karyotype of Sabanejewia bulgarica (Drensky, 1928) (Teleostei, Cobitidae) from the Danube Delta, Romania Comp. Cytogenet. (IF 1.0) Pub Date : 2023-07-05 Eva Hnátková, Zuzana Majtánová, Vendula Bohlen Šlechtová, Joerg Bohlen, Petr Ráb
The karyotype of the freshwater fish Sabanejewia bulgarica (Drensky, 1928), from the Danube Delta, was studied by conventional Giemsa staining and the C-banding technique. The diploid chromosome number was 2n = 50. The karyotype contained 2 pairs of metacentric (the first one was much larger than the second one), 6 pairs of submetacentric and 17 pairs of subtelocentric to acrocentric chromosomes
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Allium cytogenetics: a critical review on the Indian taxa Comp. Cytogenet. (IF 1.0) Pub Date : 2023-05-29 Biplab Kumar Bhowmick, Sayantika Sarkar, Dipasree Roychowdhury, Sayali D. Patil, Manoj M. Lekhak, Deepak Ohri, Satyawada Rama Rao, S. R. Yadav, R. C. Verma, Manoj K. Dhar, S. N. Raina, Sumita Jha
The genus Allium Linnaeus, 1753 (tribe Allieae) contains about 800 species worldwide of which almost 38 species are reported in India, including the globally important crops (onion, garlic, leek, shallot) and many wild species. A satisfactory chromosomal catalogue of Allium species is missing which has been considered in the review for the species occurring in India. The most prominent base number
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More hidden diversity in a cryptic species complex: a new subspecies of Leptidea sinapis (Lepidoptera, Pieridae) from Northern Iran Comp. Cytogenet. (IF 1.0) Pub Date : 2023-05-04 Vazrick Nazari, Vladimir A. Lukhtanov, Alireza Naderi, Zdenek Faltýnek Fric, Vlad Dincă, Roger Vila
A new subspecies of Leptidea sinapis from Northern Iran, discovered by means of DNA barcoding, is described as Leptidea sinapis tabarestanassp. nov. The new subspecies is allopatric with respect to other populations of L. sinapis and is genetically distinct, appearing as a well-supported sister clade to all other populations in COI-based phylogenetic reconstructions. Details on karyotype, genitalia
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Intraspecific divergence of diploid grass Aegilops comosa is associated with structural chromosome changes Comp. Cytogenet. (IF 1.0) Pub Date : 2023-04-12 Ekaterina D. Badaeva, Violetta V. Kotseruba, Andnrey V. Fisenko, Nadezhda N. Chikida, Maria Kh. Belousova, Peter M. Zhurbenko, Sergei A. Surzhikov, Alexandra Yu. Dragovich
Aegilops comosa Smith in Sibthorp et Smith, 1806 is diploid grass with MM genome constitution occurring mainly in Greece. Two morphologically distinct subspecies – Ae. c. comosa Chennaveeraiah, 1960 and Ae. c. heldreichii (Holzmann ex Boissier) Eig, 1929 are discriminated within Ae. comosa, however, genetic and karyotypic bases of their divergence are not fully understood. We used Fluorescence in
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Assessing ploidy levels and karyotype structure of the fire ant Solenopsis saevissima Smith, 1855 (Hymenoptera, Formicidae, Myrmicinae) Comp. Cytogenet. (IF 1.0) Pub Date : 2023-04-11 Ananda Ribeiro Macedo de Andrade, Danon Clemes Cardoso, Maykon Passos Cristiano
The family Formicidae is composed of ants that organize themselves into castes in which every individual has a joint organizational function. Solenopsis Westwood, 1840 is an ant genus with opportunistic and aggressive characteristics, known for being invasive species and stings that cause burning in humans. This genus is particularly difficult to classify and identify since its morphology provides
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Comparative karyotype analysis of eight Cucurbitaceae crops using fluorochrome banding and 45S rDNA-FISH Comp. Cytogenet. (IF 1.0) Pub Date : 2023-02-09 Chao-Wen She, Xiang-Hui Jiang, Chun-Ping He
To have an insight into the karyotype variation of eight Cucurbitaceae crops including Cucumis sativus Linnaeus, 1753, Cucumis melo Linnaeus, 1753, Citrullus lanatus (Thunberg, 1794) Matsumura et Nakai, 1916, Benincasa hispida (Thunberg, 1784) Cogniaux, 1881, Momordica charantia Linnaeus, 1753, Luffa cylindrica (Linnaeus, 1753) Roemer, 1846, Lagenaria siceraria var. hispida (Thunberg, 1783) Hara
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Karyotype differentiation in the Nothobranchius ugandensis species group (Teleostei, Cyprinodontiformes), seasonal fishes from the east African inland plateau, in the context of phylogeny and biogeography Comp. Cytogenet. (IF 1.0) Pub Date : 2023-01-31 Eugene Yu. Krysanov, Béla Nagy, Brian R. Watters, Alexandr Sember, Sergey A. Simanovsky
The karyotype differentiation of the twelve known members of the Nothobranchius ugandensis Wildekamp, 1994 species group is reviewed and the karyotype composition of seven of its species is described herein for the first time using a conventional cytogenetic protocol. Changes in the architecture of eukaryotic genomes often have a major impact on processes underlying reproductive isolation, adaptation
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Chromosome complements of Channa lucius and C. striata from Phu Quoc Island and karyotypic evolution in snakehead fishes (Actinopterygii, Channidae) Comp. Cytogenet. (IF 1.0) Pub Date : 2023-01-03 Denis V. Prazdnikov
Snakehead fishes of the family Channidae are obligatory air-breathers freshwater predators, the vast majority of which belong to the genus Channa Scopoli, 1777. Channa species are characterized by high karyotypic diversity due to various types of chromosomal rearrangements. It is assumed that, in addition to the lifestyle, fragmentation and isolation of snakehead populations contribute to an increase
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Chromosomal polymorphism in natural populations of Chironomus sp. prope agilis Kiknadze, Siirin, Filippova et al., 1991 (Diptera, Chironomidae) Comp. Cytogenet. (IF 1.0) Pub Date : 2022-12-16 Veronika V. Golygina
Species Chironomus sp. prope agilis Kiknadze, Siirin, Filippova et al., 1991 belongs to the Ch. plumosus group of sibling species. It was described on the basis of its karyotype and analysis of isozymes from one population in the Urals but since then no quantitative data on chromosomal polymorphism of this species have been published. The goal of this study is to broaden our knowledge of the chromosomal
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Integrative analysis reveals cryptic speciation linked to habitat differentiation within Albanian populations of the anomalous blues (Lepidoptera, Lycaenidae, Polyommatus Latreille, 1804) Comp. Cytogenet. (IF 1.0) Pub Date : 2022-11-15 Laurian Parmentier, Roger Vila, Vladimir Lukhtanov
The Balkan Peninsula is one of the greatest hotspots for biodiversity in Europe. While the region has been investigated thoroughly, some parts remain understudied and may still harbour undiscovered diversity, even in well-studied organisms such as Lepidoptera. Here we investigated the group of the so-called anomalous blue butterflies, also known as ‘brown complex’ of the subgenus Agrodiaetus Hübner
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Karyotype differentiation and male meiosis in European clades of the spider genus Pholcus (Araneae, Pholcidae) Comp. Cytogenet. (IF 1.0) Pub Date : 2022-11-02 Jiří Král, Ivalú M. Ávila Herrera, František Šťáhlavský, David Sadílek, Jaroslav Pavelka, Maria Chatzaki, Bernhard A. Huber
Haplogyne araneomorphs are a diverse spider clade. Their karyotypes are usually predominated by biarmed (i.e., metacentric and submetacentric) chromosomes and have a specific sex chromosome system, X1X2Y. These features are probably ancestral for haplogynes. Nucleolus organizer regions (NORs) spread frequently from autosomes to sex chromosomes in these spiders. This study focuses on pholcids (Pholcidae)
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The new highest number of B chromosomes (Bs) in Leisler’s bat Nyctalus leisleri (Kuhl, 1817) Comp. Cytogenet. (IF 1.0) Pub Date : 2022-09-30 Marija Rajičić, Ivana Budinski, Milan Miljević, Branka Bajić, Milan Paunović, Mladen Vujošević, Jelena Blagojević
B chromosomes (Bs) are supernumerary to the standard chromosome set, from which they prevalently derive. Variation in numbers both among individuals or populations and among cells within individuals is their constant feature. Leisler’s bat Nyctalus leisleri (Kuhl, 1817) is one of only four species of Chiroptera with detected Bs. Four males of N. leisleri were collected from two localities on the
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Karyotype and COI gene sequences of Chironomus melanotus Keyl, 1961 from the Yaroslavl region, Russia, and the difficulties with its identification using GenBank and BOLD systems Comp. Cytogenet. (IF 1.0) Pub Date : 2022-09-14 Viktor V. Bolshakov, Ekaterina A. Movergoz
Karyotype and COI gene sequences of Chironomus melanotus Keyl, 1961 from the Yaroslavl region (Russia) were analyzed. A low level of chromosomal polymorphism has been confirmed, eventually eight banding sequences were found: melA1, melB1, melC1, melD1, melE1, melF1, and melG1; only melD2 was found in two larvae from the Sunoga river. Analysis of phylogenetic tree and estimated genetic distances has
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First karyotype description of the species of Adenomera Steindachner, 1867 (Anura, Leptodactylidae) in the “ thomei” clade Comp. Cytogenet. (IF 1.0) Pub Date : 2022-08-30 Ramon Costa Dominato, Guilherme Costa de Oliveira, Carla Santana Cassini, Victor Goyannes Dill Orrico, Cléa dos Santos Ferreira Mariano, Janisete Gomes Silva
The genus Adenomera Steindachner, 1867 currently comprises 29 nominal species, some of which are suggested to be cryptic species complexes. The present study was carried out with specimens of the “thomei” clade that encompasses three taxa distributed in the Atlantic Forest biome: Adenomera thomei Almeida et Angulo, 2006, Adenomera sp. L., and Adenomera sp. M. We used classical cytogenetics to describe
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First cytogenetic data on Afrotropical lutefishes (Citharinidae) in the light of karyotype evolution in Characiformes Comp. Cytogenet. (IF 1.0) Pub Date : 2022-06-21 Sergey A. Simanovsky, Dmitry A. Medvedev, Fekadu Tefera, Alexander S. Golubtsov
The Afrotropical lutefish family Citharinidae (Citharinoidei, Characiformes) comprises three genera with eight species in total. Although Citharinidae have been studied in terms of taxonomy and systematics, no cytogenetic information was available for any representative of the family. Furthermore, only one species out of 116 in Citharinoidei (Distichodus affinis Günther, 1873) has been studied cytogenetically
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Karyotype description and comparative chromosomal mapping of rDNA and U2 snDNA sequences in Eigenmannia limbata and E. microstoma (Teleostei, Gymnotiformes, Sternopygidae) Comp. Cytogenet. (IF 1.0) Pub Date : 2022-05-05 Cristian Andrés Araya-Jaime, Duílio Mazzoni Zerbinato de Andrade Silva, Luís Ricardo Ribeiro da Silva, Cristiano Neves do Nascimento, Claudio Oliveira, Fausto Foresti
The genus Eigenmannia Jordan et Evermann,1896 includes electric fishes endemic to the Neotropical region with extensive karyotype variability and occurrence of different sex chromosome systems, however, cytogenetic studies within this group are restricted to few species. Here, we describe the karyotypes of Eigenmannia limbata (Schreiner et Miranda Ribeiro, 1903) and E. microstoma (Reinhardt, 1852)
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A critical review on cytogenetics of Cucurbitaceae with updates on Indian taxa Comp. Cytogenet. (IF 1.0) Pub Date : 2022-04-26 Biplab Kumar Bhowmick, Sumita Jha
The cytogenetic relationships in the species of Cucurbitaceae are becoming immensely important to answer questions pertaining to genome evolution. Here, a simplified and updated data resource on cytogenetics of Cucurbitaceae is presented on the basis of foundational parameters (basic, zygotic and gametic chromosome numbers, ploidy, genome size, karyotype) and molecular cytogenetics. We have revised
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Karyotypic analysis and isolation of four DNA markers of the scleractinian coral Favites pentagona (Esper, 1795) (Scleractinia, Anthozoa, Cnidaria) Comp. Cytogenet. (IF 1.0) Pub Date : 2022-04-05 Rei Kawakami, Takahiro Taguchi, Joshua Vacarizas, Masumi Ito, Takuma Mezaki, Akira Tominaga, Satoshi Kubota
We performed conventional and molecular cytogenetic studies on the Favites pentagona Esper, 1795, a scleractinian coral mostly found along the west coast of Japan. Karyotype analysis of F. pentagona by G-banding revealed a karyogram containing a homogenously staining region (HSR) on chromosome 10 in more than 50% of the examined metaphase spreads. This HSR consisted of sequences from 18S ribosomal
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Cytotaxonomic investigations on species of genus Narcissus (Amaryllidaceae) from Algeria Comp. Cytogenet. (IF 1.0) Pub Date : 2022-04-05 Naila Chahinez Boukhebache, Nabila Amirouche, Rachid Amirouche
This paper provides new cytotaxonomic data on the genus Narcissus Linnaeus, 1753, in Algeria. Populations of seven taxa, N. tazetta Linnaeus, 1753, N. pachybolbus Durieu, 1847, N. papyraceus Ker Gawler, 1806, N. elegans (Haworth) Spach, 1846, N. serotinus sensu lato Linnaeus, 1753, including N. obsoletus (Haworth) Steudel, 1841, and N. cantabricus De Candolle, 1815, were karyologically investigated
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Distribution patterns of rDNA loci in the Schedonorus-Lolium complex (Poaceae) Comp. Cytogenet. (IF 1.0) Pub Date : 2022-03-24 Helal Ahmad Ansari, Nicholas Ellison, Alan Vincent Stewart, Warren Mervyn Williams
The Schedonorus-Lolium complex of the subtribe Loliinae (Poaceae) includes several economically important forage and turf grasses. This complex encompasses Lolium Linnaeus, 1753, Festuca Linnaeus, 1753 subgenus Schedonorus (P. Beauvois, 1824) Petermann, 1849 and Micropyropsis Romero Zarco et Cabezudo, 1983. New FISH results of 5S and 18S–26S rDNA sequences are presented for three species and the
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Karyotype characteristics and gene COI sequences of Chironomus bonus Shilova et Dzhvarsheishvili, 1974 (Diptera, Chironomidae) from the South Caucasus (Republic of Georgia, Paravani river) Comp. Cytogenet. (IF 1.0) Pub Date : 2022-03-01 Mukhamed Kh. Karmokov
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Comparative cytogenetics on eight Malagasy Mantellinae (Anura, Mantellidae) and a synthesis of the karyological data on the subfamily Comp. Cytogenet. (IF 1.0) Pub Date : 2022-02-11 Marcello Mezzasalma, Franco Andreone, Gaetano Odierna, Fabio Maria Guarino, Angelica Crottini
We performed a molecular and cytogenetic analysis on different Mantellinae species and revised the available chromosomal data on this group to provide an updated assessment of its karyological diversity and evolution. Using a fragment of the mitochondrial 16S rRNA, we performed a molecular taxonomic identification of the samples that were used for cytogenetic analyses. A comparative cytogenetic analysis
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Revision of the banding sequence pool and new data on chromosomal polymorphism in natural populations of Chironomus agilis Shobanov et Djomin, 1988 (Diptera, Chironomidae) Comp. Cytogenet. (IF 1.0) Pub Date : 2021-12-17 Veronika V. Golygina, Oksana V. Ermolaeva
Quantitative and qualitative analysis of chromosomal polymorphism in 19 natural populations of Ch. agilis had been performed. Most studied populations showed a medium level of chromosomal polymorphism: on average 45±3.0% of specimens are heterozygotes with 0.52±0.01 heterozygotic inversion per larvae. Besides inversions, B-chromosomes were found in two populations. The total number of banding sequences
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Comparative chromosome studies in species of subtribe Orchidinae (Orchidaceae) Comp. Cytogenet. (IF 1.0) Pub Date : 2021-12-17 Alessio Turco, Antonella Albano, Pietro Medagli, Robert P. Wagensommer, Saverio D'Emerico
In our study, FISH mapping using 18S-5.8S-25S rDNA and 5S rDNA sequences was performed for the first time on Ophrys tenthredinifera Willdenow, 1805, Serapias vomeracea (Burman f., 1770) Briquet, 1910 and Himantoglossum hircinum (Linnaeus, 1753) Sprengel, 1826. A detailed study was also performed on O. tenthredinifera using Giemsa-staining, silver-staining, CMA fluorescence banding and fluorescence
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A new species of the genus Rhaphidosoma Amyot et Serville, 1843 (Heteroptera, Reduviidae), with data on its chromosome complement Comp. Cytogenet. (IF 1.0) Pub Date : 2021-12-15 Dmitry A. Gapon, Valentina G. Kuznetsova, Anna Maryańska-Nadachowska
A new species, Rhaphidosoma paganicumsp. nov. (Heteroptera: Reduviidae: Harpactorinae: Rhaphidosomatini), is described from the Dry Zone of Myanmar. It is the fifth species of Rhaphidosoma Amyot et Serville, 1843, known from the Oriental Region, and the first record of the genus for Myanmar and Indochina. The structure of the external and internal terminalia of the male and female is described and
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A rare chromosomal polymorphism in a Kangayam bull (Bos indicus) of south India Comp. Cytogenet. (IF 1.0) Pub Date : 2021-12-15 Vemula Harshini, P. Kumarasamy, S.M.K. Karthickeyan
A chromosomal polymorphism was detected on karyological screening of Kangayam breeding sires prior to subjecting them for frozen semen collection. One bull possessed the chromosomal complement 2n = 60, consisting of 58 acrocentric autosomes, one large sub-metacentric X-chromosome, and one small acrocentric Y-chromosome with a small visible p-arm, which was further confirmed using CBG- and GTG-banding
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Karyotype and COI gene sequence of Chironomus heteropilicornis Wülker, 1996 (Diptera, Chironomidae) from the Gydan Peninsula, Russia Comp. Cytogenet. (IF 1.0) Pub Date : 2021-12-07 Viktor V. Bolshakov, Alexander A. Prokin, Sergey V. Artemenko
The karyotype features and gene COI sequence of Chironomus heteropilicornis Wülker, 1996 from the Gydan Peninsula are presented for the first time. Nine banding sequences were determined, eight of them hpiA2, hpiB1, hpiC1, hpiC2, hpiD1, hpiE1, hpiF3 and hpiG1 were previously known from European, Georgian (South Caucasus) and Siberian populations. One new banding sequence for Ch. heteropilicornis
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Comparative cytogenetic patterns in Carangidae fishes in association with their distribution range Comp. Cytogenet. (IF 1.0) Pub Date : 2021-12-01 Rodrigo Xavier Soares, Clóvis Coutinho da Motta-Neto, Gideão Wagner Werneck Félix da Costa, Marcelo de Bello Cioffi, Luiz Antonio Carlos Bertollo, Amanda Torres Borges, Wagner Franco Molina
Carangidae are an important and widespreaded family of pelagic predatory fishes that inhabit reef regions or open ocean areas, some species occupying a vast circumglobal distribution. Cytogenetic comparisons among representatives of its different tribes help to understand the process of karyotype divergence in marine ecosystems due to the variable migratory ability of species. In this sense, conventional
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Distribution of GC-rich heterochromatin and ribosomal genes in three fungus-farming ants (Myrmicinae, Attini, Attina): insights on chromosomal evolution Comp. Cytogenet. (IF 1.0) Pub Date : 2021-11-25 Gisele Amaro Teixeira, Luísa Antônia Campos Barros, Hilton Jeferson Alves Cardoso de Aguiar, Denilce Meneses Lopes
Cytogenetic studies on fungus-farming ants have shown remarkable karyotype diversity, suggesting different chromosomal rearrangements involved in karyotype evolution in some genera. A notable cytogenetic characteristic in this ant group is the presence of GC-rich heterochromatin in the karyotypes of some ancient and derivative species. It was hypothesized that this GC-rich heterochromatin may have
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Intraspecies multiple chromosomal variations including rare tandem fusion in the Russian Far Eastern endemic evoron vole Alexandromys evoronensis (Rodentia, Arvicolinae) Comp. Cytogenet. (IF 1.0) Pub Date : 2021-11-22 Irina V. Kartavtseva, Irina N. Sheremetyeva, Marina V. Pavlenko
The vole Alexandromys evoronensis (Kovalskaya et Sokolov, 1980) with its two chromosomal races, “Evoron” (2n = 38–41, NF = 54–59) and “Argi” (2n = 34, 36, 37, NF = 51–56) is the endemic vole found in the Russian Far East. For the “Argi” chromosomal race, individuals from two isolated populations in mountain regions were investigated here for the first time using GTG-, GTC-, NOR methods. In the area
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The Kengyilia hirsuta karyotype polymorphisms as revealed by FISH with tandem repeats and single-gene probes Comp. Cytogenet. (IF 1.0) Pub Date : 2021-11-03 Xiaoyan Tao, Bo Liu, Quanwen Dou
Kengyilia hirsuta (Keng, 1959) J. L. Yang, C. Yen et B. R. Baum, 1992, a perennial hexaploidy species, is a wild relative species to wheat with great potential for wheat improvement and domestication. The genome structure and cross-species homoeology of K. hirsuta chromosomes with wheat were assayed using 14 single-gene probes covering all seven homoeologous groups, and four repetitive sequence probes
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High karyotypic variation in Orthemis Hagen, 1861 species, with insights about the neo-XY in Orthemis ambinigra Calvert, 1909 (Libellulidae, Odonata) Comp. Cytogenet. (IF 1.0) Pub Date : 2021-11-03 Liliana M. Mola, María Florencia Fourastié, Silvia Susana Agopian
The American dragonfly genus Orthemis Hagen, 1861 is mainly found in the Neotropical region. Seven of 28 taxonomically described species have been reported from Argentina. Chromosome studies performed on this genus showed a wide variation in chromosome number and a high frequency of the neoXY chromosomal sex-determination system, although the sexual pair was not observed in all cases. This work analyzes
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Derived karyotypes in two elephantfish genera (Hyperopisus and Pollimyrus): lowest chromosome number in the family Mormyridae (Osteoglossiformes) Comp. Cytogenet. (IF 1.0) Pub Date : 2021-10-08 Sergey Simanovsky, Dmitry Medvedev, Fekadu Tefera, Alexander Golubtsov
The African weakly electric elephantfish family Mormyridae comprises 22 genera and almost 230 species. Up-to-date cytogenetic information was available for 17 species representing 14 genera. Here we report chromosome number and morphology in Hyperopisus bebe (Lacepède, 1803) and Pollimyrus isidori (Valenciennes, 1847) collected from the White Nile system in southwestern Ethiopia. Both taxa displayed
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The chiasmata systems of Scottish Chysolina latecincta (Demaison, 1896) (Coleoptera, Chrysomelidae) Comp. Cytogenet. (IF 1.0) Pub Date : 2021-09-28 Eduard Petitpierre
The meiotic systems of some Scottish individuals of the rare Chrysolina latecincta ssp. intermedia (Franz, 1938) have been analyzed from meiotic cells at diakinesis to study the types of chromosomal bivalents and the number and locations of their chiasmata. The mean number of unichiasmate was about two-thirds and that of bichiasmate bivalents about one-third. Most chiasmata were at distal positions
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Study of chromatin diminution in Cyclops kolensis (Copepoda, Crustacea) by radiobiological methods Comp. Cytogenet. (IF 1.0) Pub Date : 2021-09-28 Andrey Grishanin, Oksana Chinyakova
The experimental results show that at doses of 20 Gy and 100 Gy, the development of Cyclops kolensis Lilljeborg, 1901 (Copepoda, Cyclopoida) embryos ceases at the 16-cell stage, without affecting the course of chromatin diminution. A dose of 200 Gy terminated the process of chromatin diminution in some of the embryos. These results support the hypothesis that cytoplasmic factors in the egg play an
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Comparative analysis of chromosome numbers and sex chromosome systems in Paraneoptera (Insecta) Comp. Cytogenet. (IF 1.0) Pub Date : 2021-09-27 Valentina G. Kuznetsova, Ilya A. Gavrilov-Zimin, Snejana M. Grozeva, Natalia V. Golub
This article is part (the 4th article) of the themed issue (a monograph) “Aberrant cytogenetic and reproductive patterns in the evolution of Paraneoptera”. The purpose of this article is to consider chromosome structure and evolution, chromosome numbers and sex chromosome systems, which all together constitute the chromosomal basis of reproduction and are essential for reproductive success. We are
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Aberrant ontogeneses and life cycles in Paraneoptera Comp. Cytogenet. (IF 1.0) Pub Date : 2021-08-25 Ilya A. Gavrilov-Zimin
The paper is a third part of the themed issue “Aberrant cytogenetic and reproductive patterns in the evolution of Paraneoptera”, prepared by a Russian-Bulgarian research team on the basis of long-term collaborative studies. This chapter reviews different peculiar aberrations in the ontogenesis of Paraneoptera, such as the appearance of the quiescent apodal and/or arostrate instars, exuviatrial, pupillarial
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Egg retention, viviparity and ovoviviparity in Paraneoptera Comp. Cytogenet. (IF 1.0) Pub Date : 2021-08-03 Ilya A. Gavrilov-Zimin
This article is a second part of the themed issue “Aberrant cytogenetic and reproductive patterns in the evolution of Paraneoptera insects”, prepared by the Russian-Bulgarian research team. Here, analysis of aberrations related to the egg development is provided based on literature data and the author’s own investigations. Evolutionary aspects of ovoviviparity/viviparity are also briefly discussed
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Introduction to the study of chromosomal and reproductive patterns in Paraneoptera Comp. Cytogenet. (IF 1.0) Pub Date : 2021-07-19 Ilya A. Gavrilov-Zimin, Snejana M. Grozeva, Dmitrii A. Gapon, Andrei S. Kurochkin, Katia G. Trencheva, Valentina G. Kuznetsova
This paper opens the themed issue (a monograph) “Aberrant cytogenetic and reproductive patterns in the evolution of Paraneoptera”, prepared by a Russian-Bulgarian research team on the basis of long-term collaborative studies. In this first part of the issue, we provide the basic introductory information, describe the material involved and the methods applied, and give terminology and nomenclature of
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Chromosomal and DNA barcode analysis of the Melitaea ala Staudinger, 1881 species complex (Lepidoptera, Nymphalidae) Comp. Cytogenet. (IF 1.0) Pub Date : 2021-06-18 Vladimir A. Lukhtanov, Anastasia V. Gagarina, Elena A. Pazhenkova
The species of the Melitaea ala Staudinger, 1881 complex are distributed in Central Asia. Here we show that this complex is a monophyletic group including the species, M. ala, M. kotshubeji Sheljuzhko, 1929 and M. enarea Fruhstorfer, 1917. The haploid chromosome number n=29 is found in M. ala and M. kotshubeji and is, most likely, a symplesiomorphy of the M. ala complex. We show that M. ala consists
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Differentiation of the frog sculpin Myoxocephalus stelleri Tilesius, 1811 (Actinopterygii, Cottidae) based on mtDNA and karyotype analyses Comp. Cytogenet. (IF 1.0) Pub Date : 2021-06-14 Irina N. Moreva, Olga A. Radchenko, Anna V. Petrovskaya
A molecular genetic and karyological study of the frog sculpin Myoxocephalus stelleri Tilesius, 1811 was carried out on an extensive sample from a large area of the species’ range. A total of 42 specimens was sampled from the Sea of Japan, Sea of Okhotsk, and coastal waters off the southern Kuril Islands, which makes this sampling scheme the most comprehensive to date. The level of mtDNA polymorphism
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Chromosomes of three gall wasps of the tribe Aylacini (Hymenoptera, Cynipidae) Comp. Cytogenet. (IF 1.0) Pub Date : 2021-06-04 Vladimir E. Gokhman
Chromosomes of two species of the tribe Aylacini (Cynipidae), Isocolus jaceae (Schenck, 1863) and I. scabiosae (Giraud, 1859) (both have 2n = 18) were studied for the first time. In addition, 2n = 20 is confirmed in a member of the same tribe, Aulacidea hieracii (Bouché, 1834). All chromosomes of these gall wasps are biarmed; however, they gradually decrease in size in the case of A. hieracii, whereas
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Unusual chromosomal polymorphism of the common shrew, Sorex araneus L., in southern Belarus Comp. Cytogenet. (IF 1.0) Pub Date : 2021-06-04 Iryna A. Kryshchuk, Victor N. Orlov, Elena V. Cherepanova, Yuri M. Borisov
Analysis of the frequency of karyotypes and chromosomal rearrangements in the distributional ranges of four metacentric races of Sorex araneus Linnaeus, 1758 has revealed features that are not typical for polymorphic populations of this species. The frequency of the acrocentric karyotype and heterozygotes for fusion of acrocentric chromosomes turned out to be significantly higher than expected in case
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Karyotype and COI sequences of Chironomus sokolovae Istomina, Kiknadze et Siirin, 1999 (Diptera,Chironomidae) from the bay of Orkhon River, Mongolia Comp. Cytogenet. (IF 1.0) Pub Date : 2021-05-27 Viktor V. Bolshakov, Alexander A. Prokin
Chironomus sokolovae Istomina, Kiknadze et Siirin, 1999 (Diptera, Chironomidae) is recorded from Mongolia for the first time. Eleven banding sequences determined in the Mongolian population were previously known from Altai and Yenisei populations: sokA1, sokB1, sokB2, sokC1, sokC2, sokD1, sokD2, sokE1, sokF1, sokF2 and sokG1. The additional B-chromosomes are absent. DNA-barcoding of COI gene was carried
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Karyotype and COI sequences of Chironomus sokolovae Istomina, Kiknadze et Siirin, 1999 (Diptera,Chironomidae) from the bay of Orkhon River, Mongolia Comp. Cytogenet. (IF 1.0) Pub Date : 2021-05-27 Viktor V. Bolshakov,Alexander A. Prokin
Chironomus sokolovae Istomina, Kiknadze et Siirin, 1999 (Diptera, Chironomidae) is recorded from Mongolia for the first time. Eleven banding sequences determined in the Mongolian population were previously known from Altai and Yenisei populations: sokA1, sokB1, sokB2, sokC1, sokC2, sokD1, sokD2, sokE1, sokF1, sokF2 and sokG1. The additional B-chromosomes are absent. DNA-barcoding of COI gene was carried
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Genome size variation and karyotype diversity in eight taxa of Sorbus sensu stricto (Rosaceae) from China Comp. Cytogenet. (IF 1.0) Pub Date : 2021-05-20 Jiabao Li, Kailin Zhu, Qin Wang, Xin Chen
Eight taxa of Sorbus Linnaeus, 1753 sensu stricto (Rosaceae) from China have been studied karyologically through chromosome counting, chromosomal measurement and karyotype symmetry. Genome size was also estimated by flow cytometry. Six taxa, S. amabilis Cheng ex T.T.Yu et K.C.Kuan, 1963, S. hupehensis var. paucijuga (D.K. Zang et P.C. Huang, 1992) L.T. Lu, 2000, S. koehneana C.K. Schneider, 1906, S
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Genome size variation and karyotype diversity in eight taxa of Sorbus sensu stricto (Rosaceae) from China Comp. Cytogenet. (IF 1.0) Pub Date : 2021-05-20 Jiabao Li,Kailin Zhu,Qin Wang,Xin Chen
Eight taxa of Sorbus Linnaeus, 1753 sensu stricto (Rosaceae) from China have been studied karyologically through chromosome counting, chromosomal measurement and karyotype symmetry. Genome size was also estimated by flow cytometry. Six taxa, S. amabilis Cheng ex T.T.Yu et K.C.Kuan, 1963, S. hupehensis var. paucijuga (D.K. Zang et P.C. Huang, 1992) L.T. Lu, 2000, S. koehneana C.K. Schneider, 1906, S
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Comparative study of four Mystus species (Bagridae, Siluriformes) from Thailand: insights into their karyotypic diversity Comp. Cytogenet. (IF 1.0) Pub Date : 2021-04-26 Pun Yeesin, Phichaya Buasriyot, Sukhonthip Ditcharoen, Patcharaporn Chaiyasan, Chatmongkon Suwannapoom, Sippakorn Juntaree, Sitthisak Jantarat, Sucheela Talumphai, Marcelo de Bello Cioffi, Thomas Liehr, Alongklod Tanomtong, Weerayuth Supiwong
Karyotypes of four catfishes of the genus Mystus Scopoli, 1777 (family Bagridae), M. atrifasciatus Fowler, 1937, M. mysticetus Roberts, 1992, M. singaringan (Bleeker, 1846) and M. wolffii (Bleeker, 1851), were analysed by conventional and Ag-NOR banding as well as fluorescence in situ hybridization (FISH) techniques. Microsatellite d(GC)15, d(CAA)10, d(CAT)10 and d(GAA)10 repeat probes were applied