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Correction Mol. Membr. Biol. (IF 2.857) Pub Date : 2020-10-07
(2019). Correction. Molecular Membrane Biology: Vol. 35, No. 1, pp. 86-86.
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Potassium channels and their role in glioma: A mini review. Mol. Membr. Biol. (IF 2.857) Pub Date : 2020-03-09 Jia Liu,Chao Qu,Chao Han,Meng-Meng Chen,Li-Jia An,Wei Zou
K+ channels regulate a multitude of biological processes and play important roles in a variety of diseases by controlling potassium flow across cell membranes. They are widely expressed in the central and peripheral nervous system. As a malignant tumor derived from nerve epithelium, glioma has the characteristics of high incidence, high recurrence rate, high mortality rate, and low cure rate. Since
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Uncoupling protein 2 prevents ischaemia reperfusion injury through the regulation ROS/NF-κB signalling in mice. Mol. Membr. Biol. (IF 2.857) Pub Date : 2020-01-27 Yaolei Zhang,Xin Guo,Ting Li,Yaxing Feng,Wei Li,Xiaoyan Zhu,Rui Gu,Longfu Zhou
Abstract Background and objective: Renal ischaemia reperfusion injury (IRI), characterized by excessive cell apoptosis and inflammation, remains a clinical challenge. Mitochondrial membrane potential is related to apoptosis and inflammation of IRI. Previous studies have indicated that uncoupling protein 2 (UCP2) and its receptors play an important role in inflammation, apoptosis and injuries, especially
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Spatial organization of palmitoyl acyl transferases governs substrate localization and function. Mol. Membr. Biol. (IF 2.857) Pub Date : 2020-01-22 Julie M Philippe,Paul M Jenkins
Protein palmitoylation is a critical posttranslational modification that regulates protein trafficking, localization, stability, sorting and function. In mammals, addition of this lipid modification onto proteins is mediated by a family of 23 palmitoyl acyl transferases (PATs). PATs often palmitoylate substrates in a promiscuous manner, precluding our understanding of how these enzymes achieve specificity
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Zinc uptake in the Basidiomycota: Characterization of zinc transporters in Ustilago maydis. Mol. Membr. Biol. (IF 2.857) Pub Date : 2019-10-16 Adriana M Martha-Paz,David Eide,David Mendoza-Cózatl,Norma A Castro-Guerrero,Elva T Aréchiga-Carvajal
At present, the planet faces a change in the composition and bioavailability of nutrients. Zinc deficiency is a widespread problem throughout the world. It is imperative to understand the mechanisms that organisms use to adapt to the deficiency of this micronutrient. In the Ascomycetes fungi, the ZIP family of proteins is one of the most important for zinc transport and includes high affinity Zrt1p
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The KdpFABC complex - K+ transport against all odds. Mol. Membr. Biol. (IF 2.857) Pub Date : 2019-07-22 Bjørn P Pedersen,David L Stokes,Hans-Jürgen Apell
In bacteria, K+ is used to maintain cell volume and osmotic potential. Homeostasis normally involves a network of constitutively expressed transport systems, but in K+ deficient environments, the KdpFABC complex uses ATP to pump K+ into the cell. This complex appears to be a hybrid of two types of transporters, with KdpA descending from the superfamily of K+ transporters and KdpB belonging to the superfamily
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BKCa channel is a molecular target of vitamin C to protect against ischemic brain stroke. Mol. Membr. Biol. (IF 2.857) Pub Date : 2019-05-15 Luyao Li,Shan Li,Chuanbing Hu,Li Zhou,Yujiao Zhang,Mingyan Wang,Zhi Qi
Epidemiological studies have demonstrated that vitamin C decreases the risk of stroke, which has generally been ascribed to its function as antioxidant and free radical scavenger. However, whether there is a defined molecular target for vitamin C on stroke is unknown. Utilizing middle cerebral artery occlusion (MCAO) in rats as a model for ischemic stroke, we demonstrated that long-term, low-dose administration
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Comparison between MassARRAY and pyrosequencing for CYP2C19 and ABCB1 gene variants of clopidogrel efficiency genotyping. Mol. Membr. Biol. (IF 2.857) Pub Date : 2019-04-25 Juan Liu,Zesheng Xu,Ya Li,Shipeng Dai,Junying Liu,Junjun Pan,Yang Jiang
Clopidogrel is one of the most frequently used drugs in patients to reduce cardiovascular events. Since patients with different genetic variations respond quite differently to clopidogrel therapy, the related genetic testing plays a vital role in its dosage and genetic testing related to clopidogrel therapy is currently considered as routine test worldwide. In this study, we aim to use two different
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Engineering stability in NADPH oxidases: A common strategy for enzyme production. Mol. Membr. Biol. (IF 2.857) Pub Date : 2019-01-10 Marta Ceccon,Elisa Millana Fananas,Marta Massari,Andrea Mattevi,Francesca Magnani
NADPH oxidases (NOXs) are membrane enzymes whose sole function is the generation of reactive oxygen species. Humans have seven NOX isoenzymes that feature distinct functions in immune response and cell signaling but share the same catalytic core comprising a FAD-binding dehydrogenase domain and a heme-binding transmembrane domain. We previously described a mutation that stabilizes the dehydrogenase
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Corrigendum. Mol. Membr. Biol. (IF 2.857) Pub Date : 2018-10-10
(2017). Corrigendum. Molecular Membrane Biology: Vol. 34, No. 1-2, pp. 65-65.
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Combining genetic and biophysical approaches to probe the structure and function relationships of the notch receptor. Mol. Membr. Biol. (IF 2.857) Pub Date : 2018-09-24 Martin Baron
Notch is a conserved cell signalling receptor regulating many aspects of development and tissue homeostasis. Notch is activated by ligand-induced proteolytic cleavages that release the Notch intracellular domain, which relocates to the nucleus to regulate gene transcription. Proteolytic activation first requires mechanical force to be applied to the Notch extracellular domain through an endocytic pulling
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Adaptor protein 1 B mu subunit does not contribute to the recycling of kAE1 protein in polarized renal epithelial cells. Mol. Membr. Biol. (IF 2.857) Pub Date : 2018-04-13 Ensaf Y Almomani,Nicolas Touret,Emmanuelle Cordat
Mutations in the gene encoding the kidney anion exchanger 1 (kAE1) can lead to distal renal tubular acidosis (dRTA). dRTA mutations reported within the carboxyl (C)-terminal tail of kAE1 result in apical mis-targeting of the exchanger in polarized renal epithelial cells. As kAE1 physically interacts with the μ subunit of epithelial adaptor protein 1 B (AP-1B), we investigated the role of heterologously
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Structural biology of solute carrier (SLC) membrane transport proteins. Mol. Membr. Biol. (IF 2.857) Pub Date : 2018-04-13 Xiaoyun Bai,Trevor F Moraes,Reinhart A F Reithmeier
The human solute carriers (SLCs) comprise over 400 different transporters, organized into 65 families (http://slc.bioparadigms.org/) based on their sequence homology and transport function. SLCs are responsible for transporting extraordinarily diverse solutes across biological membranes, including inorganic ions, amino acids, lipids, sugars, neurotransmitters and drugs. Most of these membrane proteins
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Bacterial outer membrane vesicles: New insights and applications. Mol. Membr. Biol. (IF 2.857) Pub Date : 2017-11-30 Deepak Anand,Arunima Chaudhuri
Outer membrane vesicles (OMVs) (∼50–250 nm in diameter) are produced by both pathogenic and nonpathogenic bacteria as a canonical end product of secretion. In this review, we focus on the OMVs produced by gram-negative bacteria. We provide an overview of the OMV structure, various factors regulating their production, and their role in modulating host immune response using a few representative examples
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Mechanism of NF-κB signaling pathway and autophagy in the regulation of osteoblast differentiation. Mol. Membr. Biol. (IF 2.857) Pub Date : 2017-11-23 Han Qin,Hong-Zhi Xu,Yong-Qing Gong
Objective: The objective of the present work was to investigate a possible mechanism of NF-κB signaling pathway and autophagy in the regulation of osteoblast differentiation, and provide experimental basis for the study of tooth eruption disorder. Methods: Mouse osteoblast-like (MC3T3-E1) cells were inoculated with a cell density of 70%. According to the grouping experimental design, Western blot and
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Regulation of stressed-induced cell death by the Bcl-2 family of apoptotic proteins. Mol. Membr. Biol. (IF 2.857) Pub Date : 2017-11-23 Rabih Roufayel
Apoptosis is often deregulated in a number of human diseases. Heat-induced apoptosis is a model system for studying the consequences of protein misfolding and is mediated by the Bcl-2 family of proteins. This family consists of both pro-apoptotic and anti-apoptotic members that control mitochondrial integrity. The BH3-only pro-apoptotic members are strong inducers of apoptotic cell death. Protein damaging
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K+-Cl- co-transporter 2 (KCC2) - a membrane trafficking perspective. Mol. Membr. Biol. (IF 2.857) Pub Date : 2017-11-16 Bor Luen Tang
K+-Cl− co-transporter 2 (KCC2/SLC12A5) is a neuronal specific cation chloride co-transporter which is active under isotonic conditions, and thus a key regulator of intracellular Cl− levels. It also has an ion transporter-independent structural role in modulating the maturation and regulation of excitatory glutamatergic synapses. KCC2 levels are developmentally regulated, and a postnatal upregulation
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The octanoylated energy regulating hormone ghrelin: An expanded view of ghrelin's biological interactions and avenues for controlling ghrelin signaling. Mol. Membr. Biol. (IF 2.857) Pub Date : 2017-11-16 Elizabeth R Cleverdon,Kayleigh R McGovern-Gooch,James L Hougland
Ghrelin is a small peptide hormone that requires a unique post-translational modification, serine octanoylation, to bind and activate the GHS-R1a receptor. Initially demonstrated to stimulate hunger and appetite, ghrelin-dependent signaling is implicated in a variety of neurological and physiological processes influencing diseases such as diabetes, obesity, and Prader-Willi syndrome. In addition to
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The partition and transport behavior of cytotoxic ionic liquids (ILs) through the DPPC bilayer: Insights from molecular dynamics simulation. Mol. Membr. Biol. (IF 2.857) Pub Date : 2017-10-13 Mokhtar Ganjali Koli,Khaled Azizi
A molecular dynamics (MD) simulation with atomistic details was performed to examine the partitioning and transport behavior of moderately cytotoxic ionic liquids (ILs), namely choline bis(2-ethylhexyl) phosphate (CBEH), choline bis(2,4,4-trimethylpentyl) phosphinate (CTMP) and choline O,O-diethyl dithiophosphate (CDEP) in a fully hydrated dipalmitoylphosphatidylcholine (DPPC) bilayer in the fluid
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Membrane-bound pyrophosphatase of human gut microbe Clostridium methylpentosum confers improved salt tolerance in Escherichia coli, Saccharomyces cerevisiae and tobacco. Mol. Membr. Biol. (IF 2.857) Pub Date : 2017-10-13 Yumei Yang,Yanjuan Liu,Hang Yuan,Xian Liu,Yanxiu Gao,Ming Gong,Zhurong Zou
Membrane-bound pyrophosphatases (PPases) are involved in the adaption of organisms to stress conditions, which was substantiated by numerous plant transgenic studies with H+-PPase yet devoid of any correlated evidences for other two subfamilies, Na+-PPase and Na+,H+-PPase. Herein, we demonstrate the gene cloning and functional evaluation of the membrane-bound PPase (CmPP) of the human gut microbe Clostridium
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pH-sensitive pHLIP® coated niosomes. Mol. Membr. Biol. (IF 2.857) Pub Date : 2017-08-09 Mohan C Pereira,Monica Pianella,Da Wei,Anna Moshnikova,Carlotta Marianecci,Maria Carafa,Oleg A Andreev,Yana K Reshetnyak
Nanomedicine is becoming very popular over conventional methods due to the ability to tune physico-chemical properties of nano vectors, which are used for encapsulation of therapeutic and diagnostic agents. However, the success of nanomedicine primarily relies on how specifically and efficiently nanocarriers can target pathological sites to minimize undesirable side effects and enhance therapeutic
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Curcumin inhibits oxidative stress-induced TRPM2 channel activation, calcium ion entry and apoptosis values in SH-SY5Y neuroblastoma cells: Involvement of transfection procedure. Mol. Membr. Biol. (IF 2.857) Pub Date : 2017-06-01 Ahmi Öz,Ömer Çelik
Transient Receptor Potential (TRP) channels are mostly Ca2+ permeable cation channels. Transient Receptor Potential Melastatin-like 2 (TRPM2) is expressed in neurological tissues such as brain, dorsal root ganglia (DRG) neurons, hippocampus and also liver, heart and kidney. The SH-SY5Y cells are mostly used as a cellular model of neurodegenerative diseases, Alzheimer's and Parkinson's diseases. Curcumin
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Secretome derived from breast tumor cell lines alters the morphology of human umbilical vein endothelial cells. Mol. Membr. Biol. (IF 2.857) Pub Date : 2016-09-30 Erika Olivia Gómez,Yolanda Irasema Chirino,Norma Laura Delgado-Buenrostro,Alejandro López-Saavedra,Noemí Meraz-Cruz,Rebeca López-Marure
Metastases, responsible for most of the solid tumor associated deaths, require angiogenesis and changes in endothelial cells. In this work, the effect of the secretomes of three breast tumor cell lines (MCF-7, MDA-MB-231 and ZR-75-30) on human umbilical vein endothelial cells (HUVEC) morphology was investigated. HUVEC treated with secretomes from breast cells were analyzed by confocal and time-lapse
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The changing landscape of membrane protein structural biology through developments in electron microscopy. Mol. Membr. Biol. (IF 2.857) Pub Date : 2016-09-09 Shaun Rawson,Simon Davies,Jonathan D Lippiat,Stephen P Muench
Membrane proteins are ubiquitous in biology and are key targets for therapeutic development. Despite this, our structural understanding has lagged behind that of their soluble counterparts. This review provides an overview of this important field, focusing in particular on the recent resurgence of electron microscopy (EM) and the increasing role it has to play in the structural studies of membrane
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Molecular mechanisms linking geranylgeranyl diphosphate synthase to cell survival and proliferation. Mol. Membr. Biol. (IF 2.857) Pub Date : 2016-08-18 Sherry S Agabiti,Yilan Liang,Andrew J Wiemer
Geranylgeranyl diphosphate is a 20-carbon isoprenoid phospholipid whose lipid moiety can be post-translationally incorporated into proteins to promote membrane association. The process of geranylgeranylation has been implicated in anti-proliferative effects of clinical agents that inhibit enzymes of the mevalonate pathway (i.e. statins and nitrogenous bisphosphonates) as well as experimental agents
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The influence of NBD fluorescent probe on model membranes containing POPC and DPPC. Mol. Membr. Biol. (IF 2.857) Pub Date : 2016-07-25 Chi-Jung Weng,Ju-Ping Wu,Ming-Yen Kuo,Ya-Wei Hsueh
To investigate the effect of fluorescent probe on the properties of membranes, we studied model membranes composed of 1,2- dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1-palmitoyl 2-oleoyl-sn-glycero-3-phosphocholine (POPC) in the presence and absence of fluorescent probe. The morphology of giant unilamellar vesicles (GUVs) has been observed as a function of temperature and composition by fluorescence
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Editorial Board Mol. Membr. Biol. (IF 2.857) Pub Date : 2016-06-16
(2015). Editorial Board. Molecular Membrane Biology: Vol. 32, No. 5-8, pp. ebi-ebi.
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Slippery signaling: Palmitoylation-dependent control of neuronal kinase localization and activity. Mol. Membr. Biol. (IF 2.857) Pub Date : 2016-05-31 Audrey Montersino,Gareth M Thomas
Modification of proteins with the lipid palmitate, a process called palmitoylation, is important for the normal function of neuronal cells. However, most attention has focused on how palmitoylation regulates the targeting and trafficking of neurotransmitter receptors and non-enzymatic scaffold proteins. In this review we discuss recent studies that suggest that palmitoylation also plays additional
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Electrostatic networks control plug stabilization in the PapC usher. Mol. Membr. Biol. (IF 2.857) Pub Date : 2016-05-16 Thieng Pham,Nadine S Henderson,Glenn T Werneburg,David G Thanassi,Anne H Delcour
The PapC usher, a β-barrel pore in the outer membrane of uropathogenic Escherichia coli, is used for assembly of the P pilus, a key virulence factor in bacterial colonization of human kidney cells. Each PapC protein is composed of a 24-stranded β-barrel channel, flanked by N- and C-terminal globular domains protruding into the periplasm, and occluded by a plug domain (PD). The PD is displaced from
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The influence of an antitumor lipid - erucylphosphocholine - on artificial lipid raft system modeled as Langmuir monolayer. Mol. Membr. Biol. (IF 2.857) Pub Date : 2016-02-25 Anita Wnętrzak,Kazimierz Łątka,Katarzyna Makyła-Juzak,Joanna Zemla,Patrycja Dynarowicz-Łątka
Outer layer of cellular membrane contains ordered domains enriched in cholesterol and sphingolipids, called ‘lipid rafts’, which play various biological roles, i.e., are involved in the induction of cell death by apoptosis. Recent studies have shown that these domains may constitute binding sites for selected drugs. For example alkylphosphocholines (APCs), which are new-generation antitumor agents
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Deuterated detergents for structural and functional studies of membrane proteins: Properties, chemical synthesis and applications. Mol. Membr. Biol. (IF 2.857) Pub Date : 2016-02-24 Kazumi Hiruma-Shimizu,Hiroki Shimizu,Gary S Thompson,Arnout P Kalverda,Simon G Patching
Detergents are amphiphilic compounds that have crucial roles in the extraction, purification and stabilization of integral membrane proteins and in experimental studies of their structure and function. One technique that is highly dependent on detergents for solubilization of membrane proteins is solution-state NMR spectroscopy, where detergent micelles often serve as the best membrane mimetic for
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Solid-state NMR structures of integral membrane proteins. Mol. Membr. Biol. (IF 2.857) Pub Date : 2016-02-08 Simon G Patching
Solid-state NMR is unique for its ability to obtain three-dimensional structures and to measure atomic-resolution structural and dynamic information for membrane proteins in native lipid bilayers. An increasing number and complexity of integral membrane protein structures have been determined by solid-state NMR using two main methods. Oriented sample solid-state NMR uses macroscopically aligned lipid