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  • NF-κB and tPA Signaling in Kidney and Other Diseases
    Cells (IF 5.656) Pub Date : 2020-05-29
    Samantha White; Ling Lin; Kebin Hu

    The activation of the nuclear factor-κB (NF-κB) pathway plays a central role in the initiation and progression of inflammation, which contributes to the pathogenesis and progression of various human diseases including kidney, brain, and other diseases. Tissue plasminogen activator (tPA), a serine protease regulating homeostasis of blood coagulation, fibrinolysis, and matrix degradation, has been shown

    更新日期:2020-05-29
  • Non-Human Primate iPSC Generation, Cultivation, and Cardiac Differentiation under Chemically Defined Conditions
    Cells (IF 5.656) Pub Date : 2020-05-29
    Michael Stauske; Ignacio Rodriguez Polo; Wadim Haas; Debbra Yasemin Knorr; Thomas Borchert; Katrin Streckfuss-Bömeke; Ralf Dressel; Iris Bartels; Malte Tiburcy; Wolfram-Hubertus Zimmermann; Rüdiger Behr

    Non-human primates (NHP) are important surrogate models for late preclinical development of advanced therapy medicinal products (ATMPs), including induced pluripotent stem cell (iPSC)-based therapies, which are also under development for heart failure repair. For effective heart repair by remuscularization, large numbers of cardiomyocytes are required, which can be obtained by efficient differentiation

    更新日期:2020-05-29
  • Generation of Multipotent Stem Cells from Adult Human Peripheral Blood Following the Treatment with Platelet-Derived Mitochondria
    Cells (IF 5.656) Pub Date : 2020-05-29
    Haibo Yu; Wei Hu; Xiang Song; Yong Zhao

    Autologous stem cells are highly preferred for cellular therapy to treat human diseases. Mitochondria are organelles normally located in cytoplasm. Our recent studies demonstrated the differentiation of adult peripheral blood-derived insulin-producing cells (designated PB-IPC) into hematopoietic-like cells after the treatment with platelet-derived mitochondria. To further explore the molecular mechanism

    更新日期:2020-05-29
  • Centering and Shifting of Centrosomes in Cells
    Cells (IF 5.656) Pub Date : 2020-05-29
    Anton V. Burakov; Elena S. Nadezhdina

    Centrosomes have a nonrandom localization in the cells: either they occupy the centroid of the zone free of the actomyosin cortex or they are shifted to the edge of the cell, where their presence is justified from a functional point of view, for example, to organize additional microtubules or primary cilia. This review discusses centrosome placement options in cultured and in situ cells. It has been

    更新日期:2020-05-29
  • Blocking the Bromodomains Function Contributes to Disturbances in Alga Chara vulgaris Spermatids Differentiation
    Cells (IF 5.656) Pub Date : 2020-05-29
    Agnieszka Wojtczak

    Bromodomain containing (BRD) proteins play an essential role in many cellular processes. The aim of this study was to estimate activity of bromodomains during alga Chara vulgaris spermatids differentiation. The effect of a bromodomain inhibitor, JQ1 (100 µM), on the distribution of individual stages of spermatids and their ultrastructure was studied. The material was Feulgen stained and analysed in

    更新日期:2020-05-29
  • Algae-Bacteria Consortia as a Strategy to Enhance H2 Production
    Cells (IF 5.656) Pub Date : 2020-05-29
    Neda Fakhimi; David Gonzalez-Ballester; Emilio Fernández; Aurora Galván; Alexandra Dubini

    Biological hydrogen production by microalgae is a potential sustainable, renewable and clean source of energy. However, many barriers limiting photohydrogen production in these microorganisms remain unsolved. In order to explore this potential and make biohydrogen industrially affordable, the unicellular microalga Chlamydomonas reinhardtii is used as a model system to solve barriers and identify new

    更新日期:2020-05-29
  • The Vasopressin Receptor 2 Mutant R137L Linked to the Nephrogenic Syndrome of Inappropriate Antidiuresis (NSIAD) Signals through an Alternative Pathway that Increases AQP2 Membrane Targeting Independently of S256 Phosphorylation
    Cells (IF 5.656) Pub Date : 2020-05-29
    Marianna Ranieri; Maria Venneri; Tommaso Pellegrino; Mariangela Centrone; Annarita Di Mise; Susanna Cotecchia; Grazia Tamma; Giovanna Valenti

    NSIAD is a rare X-linked condition, caused by activating mutations in the AVPR2 gene coding for the vasopressin V2 receptor (V2R) associated with hyponatremia, despite undetectable plasma vasopressin levels. We have recently provided in vitro evidence that, compared to V2R-wt, expression of activating V2R mutations R137L, R137C and F229V cause a constitutive redistribution of the AQP2 water channel

    更新日期:2020-05-29
  • Molecular Dissection of Neurodevelopmental Disorder-Causing Mutations in CYFIP2
    Cells (IF 5.656) Pub Date : 2020-05-29
    Matthias Schaks; Michael Reinke; Walter Witke; Klemens Rottner

    Actin remodeling is frequently regulated by antagonistic activities driving protrusion and contraction downstream of Rac and Rho small GTPases, respectively. WAVE regulatory complex (WRC), which primarily operates downstream of Rac, plays pivotal roles in neuronal morphogenesis. Recently, two independent studies described de novo mutations in the CYFIP2 subunit of WRC, which caused intellectual disability

    更新日期:2020-05-29
  • PIWIL3 Forms a Complex with TDRKH in Mammalian Oocytes
    Cells (IF 5.656) Pub Date : 2020-05-29
    Minjie Tan; Helena T.A. van Tol; David Rosenkranz; Elke F. Roovers; Mirjam J. Damen; Tom A.E. Stout; Wei Wu; Bernard A.J. Roelen

    P-element induced wimpy testis (PIWIs) are crucial guardians of genome integrity, particularly in germ cells. While mammalian PIWIs have been primarily studied in mouse and rat, a homologue for the human PIWIL3 gene is absent in the Muridae family, and hence the unique function of PIWIL3 in germ cells cannot be effectively modeled by mouse knockouts. Herein, we investigated the expression, distribution

    更新日期:2020-05-29
  • Reactive Oxygen Species and Redox Signaling in Chronic Kidney Disease
    Cells (IF 5.656) Pub Date : 2020-05-28
    Maria V. Irazabal; Vicente E. Torres

    Chronic kidney disease (CKD) remains a worldwide public health problem associated with serious complications and increased mortality rates. Accumulating evidence indicates that elevated intracellular levels of reactive oxygen species (ROS) play a major role in the pathogenesis of CKD. Increased intracellular levels of ROS can lead to oxidation of lipids, DNA, and proteins, contributing to cellular

    更新日期:2020-05-28
  • Umbilical Cord MSCs and Their Secretome in the Therapy of Arthritic Diseases: A Research and Industrial Perspective
    Cells (IF 5.656) Pub Date : 2020-05-28
    Chiara Arrigoni; Daniele D’Arrigo; Valeria Rossella; Christian Candrian; Veronica Albertini; Matteo Moretti

    The prevalence of arthritic diseases is increasing in developed countries, but effective treatments are currently lacking. The injection of mesenchymal stem cells (MSCs) represents a promising approach to counteract the degenerative and inflammatory environment characterizing those pathologies, such as osteoarthritis (OA). However, the majority of clinical approaches based on MSCs are used within an

    更新日期:2020-05-28
  • Migration Inhibitory Factor in Conditioned Medium from Human Umbilical Cord Blood-Derived Mesenchymal Stromal Cells Stimulates Hair Growth
    Cells (IF 5.656) Pub Date : 2020-05-28
    Hyun Ah Oh; Jihye Kwak; Beom Joon Kim; Hye Jin Jin; Won Seok Park; Soo Jin Choi; Wonil Oh; Soyoun Um

    Conventional therapeutic applications of mesenchymal stromal cells (MSCs) focus on cell replacement and differentiation; however, increasing evidence suggests that most of their therapeutic effects are carried out by their various secretions. This study investigated the application of conditioned medium (CM) from human umbilical cord blood-derived MSCs (hUCB-MSCs) to improve hair growth and developed

    更新日期:2020-05-28
  • Mitochondrial MicroRNAs in Aging and Neurodegenerative Diseases
    Cells (IF 5.656) Pub Date : 2020-05-28
    Albin John; Aaron Kubosumi; P. Hemachandra Reddy

    MicroRNAs (miRNAs) are important regulators of several biological processes, such as cell growth, cell proliferation, embryonic development, tissue differentiation, and apoptosis. Currently, over 2000 mammalian miRNAs have been reported to regulate these biological processes. A subset of microRNAs was found to be localized to human mitochondria (mitomiRs). Through years of research, over 400 mitomiRs

    更新日期:2020-05-28
  • Deletion of Perilipin 5 Protects Against Hepatic Injury in Nonalcoholic Fatty Liver Disease via Missing Inflammasome Activation
    Cells (IF 5.656) Pub Date : 2020-05-28
    Anastasia Asimakopoulou; Kathrin M. Engel; Nikolaus Gassler; Thilo Bracht; Barbara Sitek; Eva M. Buhl; Stavroula Kalampoka; Manuela Pinoé-Schmidt; Josef van Helden; Jürgen Schiller; Ralf Weiskirchen

    Nonalcoholic fatty liver disease (NAFLD) is a leading cause of chronic liver diseases with an increasing prevalence due to rising rates of obesity, metabolic syndrome and type II diabetes. Untreated NAFLD may progress to steatohepatitis (NASH) and ultimately liver cirrhosis. NAFLD is characterized by lipid accumulation, and when sufficient excess lipids are obtained, irreversible liver injury may follow

    更新日期:2020-05-28
  • Multiple Functions of Fubp1 in Cell Cycle Progression and Cell Survival
    Cells (IF 5.656) Pub Date : 2020-05-28
    Mingyu Kang; Hyeon Ji Kim; Tae-Jun Kim; Jin-Seok Byun; Jae-Ho Lee Deok Heon Lee; Wanil Kim; Do-Yeon Kim

    The discovery of novel and critical genes implicated in malignant development is a topic of high interest in cancer research. Intriguingly, a group of genes named “double-agent” genes were reported to have both oncogenic and tumor-suppressive functions. To date, less than 100 “double-agent” genes have been documented. Fubp1 is a master transcriptional regulator of a subset of genes by interacting with

    更新日期:2020-05-28
  • Retinal Ganglion Cell Survival and Axon Regeneration after Optic Nerve Transection is Driven by Cellular Intravitreal Sciatic Nerve Grafts
    Cells (IF 5.656) Pub Date : 2020-05-27
    Zubair Ahmed; Ellen L. Suggate; Ann Logan; Martin Berry

    Neurotrophic factors (NTF) secreted by Schwann cells in a sciatic nerve (SN) graft promote retinal ganglion cell (RGC) axon regeneration after either transplantation into the vitreous body of the eye or anastomosis to the distal stump of a transected optic nerve. In this study, we investigated the neuroprotective and growth stimulatory properties of SN grafts in which Schwann cells had been killed

    更新日期:2020-05-27
  • The Network of Angiotensin Receptors in Breast Cancer
    Cells (IF 5.656) Pub Date : 2020-05-27
    Filippo Acconcia

    The renin-angiotensin system (RAS) is a network of proteins regulating many aspects of human physiology, including cardiovascular, pulmonary, and immune system physiology. The RAS is a complicated network of G-protein coupled receptors (GPCRs) (i.e., AT1R, AT2R, MASR, and MRGD) orchestrating the effects of several hormones (i.e., angiotensin II, angiotensin (1–7), and alamandine) produced by protease-based

    更新日期:2020-05-27
  • Transposon-Based CAR T Cells in Acute Leukemias: Where are We Going?
    Cells (IF 5.656) Pub Date : 2020-05-27
    Chiara F Magnani; Sarah Tettamanti; Gaia Alberti; Ilaria Pisani; Andrea Biondi; Marta Serafini; Giuseppe Gaipa

    Chimeric Antigen Receptor (CAR) T-cell therapy has become a new therapeutic reality for refractory and relapsed leukemia patients and is also emerging as a potential therapeutic option in solid tumors. Viral vector-based CAR T-cells initially drove these successful efforts; however, high costs and cumbersome manufacturing processes have limited the widespread clinical implementation of CAR T-cell therapy

    更新日期:2020-05-27
  • RELMα is Induced in Airway Epithelial Cells by Oncostatin M Without Requirement of STAT6 or IL-6 in Mouse Lungs In Vivo
    Cells (IF 5.656) Pub Date : 2020-05-27
    Lilian Ho; Ashley Yip; Francis Lao; Fernando Botelho; Carl D. Richards

    Resistin-like molecule alpha (RELMα) and YM-1 are secreted proteins implicated in murine models of alternatively activated macrophage (AA/M2) accumulation and Th2-skewed inflammation. Since the gp130 cytokine Oncostatin M (OSM) induces a Th2-like cytokine and AA/M2 skewed inflammation in mouse lung, we here investigated regulation of RELMα and YM-1. Transient pulmonary overexpression of OSM by Adenovirus

    更新日期:2020-05-27
  • The Pluripotency Factor Nanog Protects against Neuronal Amyloid β-Induced Toxicity and Oxidative Stress through Insulin Sensitivity Restoration
    Cells (IF 5.656) Pub Date : 2020-05-27
    Ching-Chi Chang; Hsin-Hua Li; Sing-Hua Tsou; Hui-Chih Hung; Guang-Yaw Liu; Tatiana A. Korolenko; Te-Jen Lai; Ying-Jui Ho; Chih-Li Lin

    Amyloid β (Aβ) is a peptide fragment of the amyloid precursor protein that triggers the progression of Alzheimer’s Disease (AD). It is believed that Aβ contributes to neurodegeneration in several ways, including mitochondria dysfunction, oxidative stress and brain insulin resistance. Therefore, protecting neurons from Aβ-induced neurotoxicity is an effective strategy for attenuating AD pathogenesis

    更新日期:2020-05-27
  • Targeted Regulation of Nuclear Lamins by Ubiquitin and Ubiquitin-Like Modifiers
    Cells (IF 5.656) Pub Date : 2020-05-27
    Michael Blank

    Nuclear lamins (NLs) are essential components of the animal cell nucleus involved in the regulation of a plethora of molecular and cellular processes. These include the nuclear envelope assembly and stability, mechanotransduction and chromatin organization, transcription, DNA replication, damage repair, and genomic integrity maintenance. Mutations in NLs can lead to the development of a wide range

    更新日期:2020-05-27
  • Endothelial TRPV1 as an Emerging Molecular Target to Promote Therapeutic Angiogenesis
    Cells (IF 5.656) Pub Date : 2020-05-27
    Sharon Negri; Pawan Faris; Vittorio Rosti; Maria Rosa Antognazza; Francesco Lodola; Francesco Moccia

    Therapeutic angiogenesis represents an emerging strategy to treat ischemic diseases by stimulating blood vessel growth to rescue local blood perfusion. Therefore, injured microvasculature may be repaired by stimulating resident endothelial cells or circulating endothelial colony forming cells (ECFCs) or by autologous cell-based therapy. Endothelial Ca2+ signals represent a crucial player in angiogenesis

    更新日期:2020-05-27
  • Membrane Elastic Properties During Neural Precursor Cell Differentiation
    Cells (IF 5.656) Pub Date : 2020-05-26
    Juliana Soares; Glauber R. de S. Araujo; Cintia Santana; Diana Matias; Vivaldo Moura-Neto; Marcos Farina; Susana Frases; Nathan B. Viana; Luciana Romão; H. Moysés Nussenzveig; Bruno Pontes

    Neural precursor cells differentiate into several cell types that display distinct functions. However, little is known about how cell surface mechanics vary during the differentiation process. Here, by precisely measuring membrane tension and bending modulus, we map their variations and correlate them with changes in neural precursor cell morphology along their distinct differentiation fates. Both

    更新日期:2020-05-26
  • Cisplatin Protein Binding Partners and Their Relevance for Platinum Drug Sensitivity
    Cells (IF 5.656) Pub Date : 2020-05-26
    Sophie Möltgen; Eleonora Piumatti; Giuseppe M. Massafra; Sabine Metzger; Ulrich Jaehde; Ganna V. Kalayda

    Cisplatin is a widely used drug in the treatment of various solid tumors, such as ovarian cancer. However, while the acquired resistance significantly limits the success of therapy, some tumors, such as colorectal cancer, are intrinsically insensitive to cisplatin. Only a small amount of intracellular platinum binds to the target—genomic DNA. The fate of the remaining drug is largely obscure. This

    更新日期:2020-05-26
  • The Survey of Cells Responsible for Heterotopic Ossification Development in Skeletal Muscles—Human and Mouse Models
    Cells (IF 5.656) Pub Date : 2020-05-26
    Łukasz Pulik; Bartosz Mierzejewski; Maria A. Ciemerych; Edyta Brzóska; Paweł Łęgosz

    Heterotopic ossification (HO) manifests as bone development in the skeletal muscles and surrounding soft tissues. It can be caused by injury, surgery, or may have a genetic background. In each case, its development might differ, and depending on the age, sex, and patient’s conditions, it could lead to a more or a less severe outcome. In the case of the injury or surgery provoked ossification development

    更新日期:2020-05-26
  • The Paracrine Role of Endothelial Cells in Bone Formation via CXCR4/SDF-1 Pathway
    Cells (IF 5.656) Pub Date : 2020-05-26
    Tal Tamari; Rawan Kawar-Jaraisy; Ofri Doppelt; Ben Giladi; Nadin Sabbah; Hadar Zigdon-Giladi

    Vascularization is a prerequisite for bone formation. Endothelial progenitor cells (EPCs) stimulate bone formation by creating a vascular network. Moreover, EPCs secrete various bioactive molecules that may regulate bone formation. The aim of this research was to shed light on the pathways of EPCs in bone formation. In a subcutaneous nude mouse ectopic bone model, the transplantation of human EPCs

    更新日期:2020-05-26
  • Kidney Organoids and Tubuloids
    Cells (IF 5.656) Pub Date : 2020-05-26
    Fjodor A. Yousef Yengej; Jitske Jansen; Maarten B. Rookmaaker; Marianne C. Verhaar; Hans Clevers

    In the past five years, pluripotent stem cell (PSC)-derived kidney organoids and adult stem or progenitor cell (ASC)-based kidney tubuloids have emerged as advanced in vitro models of kidney development, physiology, and disease. PSC-derived organoids mimic nephrogenesis. After differentiation towards the kidney precursor tissues ureteric bud and metanephric mesenchyme, their reciprocal interaction

    更新日期:2020-05-26
  • Role of microRNA 690 in Mediating Angiotensin II Effects on Inflammation and Endoplasmic Reticulum Stress
    Cells (IF 5.656) Pub Date : 2020-05-26
    Kalhara R. Menikdiwela; Latha Ramalingam; Mostafa M. Abbas; Halima Bensmail; Shane Scoggin; Nishan S. Kalupahana; Asha Palat; Preethi Gunaratne; Naima Moustaid-Moussa

    Overactivation of the renin–angiotensin system (RAS) during obesity disrupts adipocyte metabolic homeostasis and induces endoplasmic reticulum (ER) stress and inflammation; however, underlying mechanisms are not well known. We propose that overexpression of angiotensinogen (Agt), the precursor protein of RAS in adipose tissue or treatment of adipocytes with Angiotensin II (Ang II), RAS bioactive hormone

    更新日期:2020-05-26
  • Parallel Acquisition of Plasma Membrane Ultrastructure and Cytosolic Protein Localisation in Cultured Cells via Correlated Immunogold SEM
    Cells (IF 5.656) Pub Date : 2020-05-26
    Isabell Begemann; Ulrike Keller; Harald Nüsse; Jürgen Klingauf; Milos Galic

    Scanning electron microscopy (SEM) takes advantage of distinct detectors to visualise secondary and back-scattering electrons. Here, we report an integrated approach that relies on these two detection methods to simultaneously acquire correlated information on plasma membrane topography and curvature-sensitive cytosolic protein localization in intact cell samples. We further provide detailed preparation

    更新日期:2020-05-26
  • Anti-Inflammatory Performance of Lactose-Modified Chitosan and Hyaluronic Acid Mixtures in an In Vitro Macrophage-Mediated Inflammation Osteoarthritis Model
    Cells (IF 5.656) Pub Date : 2020-05-26
    Elena Tarricone; Elena Mattiuzzo; Elisa Belluzzi; Rossella Elia; Andrea Benetti; Rina Venerando; Vincenzo Vindigni; Pietro Ruggieri; Paola Brun

    The development and progression of osteoarthritis (OA) is associated with macrophage-mediated inflammation that generates a broad spectrum of cytokines and reactive oxygen species (ROS). This study investigates the effects of mid-MW hyaluronic acid (HA) in combination with a lactose-modified chitosan (CTL), on pro-inflammatory molecules and metalloproteinases (MMPs) expression, using an in vitro model

    更新日期:2020-05-26
  • Crucial Role of Lamin A/C in the Migration and Differentiation of MSCs in Bone
    Cells (IF 5.656) Pub Date : 2020-05-26
    Natividad Alcorta-Sevillano; Iratxe Macías; Clara I. Rodríguez; Arantza Infante

    Lamin A/C, intermediate filament proteins from the nuclear lamina encoded by the LMNA gene, play a central role in mediating the mechanosignaling of cytoskeletal forces into nucleus. In fact, this mechanotransduction process is essential to ensure the proper functioning of other tasks also mediated by lamin A/C: the structural support of the nucleus and the regulation of gene expression. In this way

    更新日期:2020-05-26
  • Cancer Stem Cell Functions in Hepatocellular Carcinoma and Comprehensive Therapeutic Strategies
    Cells (IF 5.656) Pub Date : 2020-05-26
    Yu-Chin Liu; Chau-Ting Yeh; Kwang-Huei Lin

    Hepatocellular carcinoma (HCC) is a significant cause of cancer-related mortality owing to resistance to traditional treatments and tumor recurrence after therapy, which leads to poor therapeutic outcomes. Cancer stem cells (CSC) are a small subset of tumor cells with the capability to influence self-renewal, differentiation, and tumorigenesis. A number of surface markers for liver cancer stem cell

    更新日期:2020-05-26
  • CUL4-DDB1-CRBN E3 Ubiquitin Ligase Regulates Proteostasis of ClC-2 Chloride Channels: Implication for Aldosteronism and Leukodystrophy
    Cells (IF 5.656) Pub Date : 2020-05-26
    Ssu-Ju Fu; Meng-Chun Hu; Yi-Jheng Peng; Hsin-Yu Fang; Cheng-Tsung Hsiao; Tsung-Yu Chen; Chung-Jiuan Jeng; Chih-Yung Tang

    Voltage-gated ClC-2 channels are essential for chloride homeostasis. Complete knockout of mouse ClC-2 leads to testicular degeneration and neuronal myelin vacuolation. Gain-of-function and loss-of-function mutations in the ClC-2-encoding human CLCN2 gene are linked to the genetic diseases aldosteronism and leukodystrophy, respectively. The protein homeostasis (proteostasis) mechanism of ClC-2 is currently

    更新日期:2020-05-26
  • Heterogeneity of Metabolic Vulnerability in Imatinib -Resistant Gastrointestinal Stromal Tumor
    Cells (IF 5.656) Pub Date : 2020-05-26
    Wen-Kuan Huang; Jiwei Gao; Ziqing Chen; Hao Shi; Juan Yuan; Huanhuan L. Cui; Chun-Nan Yeh; Robert Bränström; Catharina Larsson; Shuijie Li; Weng-Onn Lui

    Metabolic reprogramming is a hallmark of cancer cells in response to targeted therapy. Decreased glycolytic activity with enhanced mitochondrial respiration secondary to imatinib has been shown in imatinib-sensitive gastrointestional stromal tumors (GIST). However, the role of energy metabolism in imatinib-resistant GIST remains poorly characterized. Here, we investigated the effect of imatinib treatment

    更新日期:2020-05-26
  • Metabolomic Profiling of Plasma and Erythrocytes in Sickle Mice Points to Altered Nociceptive Pathways
    Cells (IF 5.656) Pub Date : 2020-05-26
    Klétigui Casimir Dembélé; Thomas Mintz; Charlotte Veyrat-Durebex; Floris Chabrun; Stéphanie Chupin; Lydie Tessier; Gilles Simard; Daniel Henrion; Delphine Mirebeau-Prunier; Juan Manuel Chao de la Barca; Pierre-Louis Tharaux; Pascal Reynier

    Few data-driven metabolomic approaches have been reported in sickle cell disease (SCD) to date. We performed a metabo-lipidomic study on the plasma and red blood cells of a steady-state mouse model carrying the homozygous human hemoglobin SS, compared with AS and AA genotypes. Among the 188 metabolites analyzed by a targeted quantitative metabolomic approach, 153 and 129 metabolites were accurately

    更新日期:2020-05-26
  • Phenotyping the Chilling and Freezing Responses of Young Microspore Stage Wheat Spikes Using Targeted Metabolome and Lipidome Profiling
    Cells (IF 5.656) Pub Date : 2020-05-25
    Bo Eng Cheong; Olive Onyemaobi; William Wing Ho Ho; Thomas Ben Biddulph; Thusitha W. T. Rupasinghe; Ute Roessner; Rudy Dolferus

    Chilling and frost conditions impose major yield restraints to wheat crops in Australia and other temperate climate regions. Unpredictability and variability of field frost events are major impediments for cold tolerance breeding. Metabolome and lipidome profiling were used to compare the cold response in spikes of cold-tolerant Young and sensitive variety Wyalkatchem at the young microspore (YM) stage

    更新日期:2020-05-25
  • Ascorbic Acid Promotes Functional Restoration after Spinal Cord Injury Partly by Epigenetic Modulation
    Cells (IF 5.656) Pub Date : 2020-05-25
    Jin Young Hong; Ganchimeg Davaa; Hyunjin Yoo; Kwonho Hong; Jung Keun Hyun

    Axonal regeneration after spinal cord injury (SCI) is difficult to achieve, and no fundamental treatment can be applied in clinical settings. DNA methylation has been suggested to play a role in regeneration capacity and neuronal growth after SCI by controlling the expression of regeneration-associated genes (RAGs). The aim of this study was to examine changes in neuronal DNA methylation status after

    更新日期:2020-05-25
  • Platelet Transforming Growth Factor-β1 Induces Liver Sinusoidal Endothelial Cells to Secrete Interleukin-6
    Cells (IF 5.656) Pub Date : 2020-05-25
    Alexandre Balaphas; Jeremy Meyer; Remo Perozzo; Magali Zeisser-Labouebe; Sarah Berndt; Antoine Turzi; Pierre Fontana; Leonardo Scapozza; Carmen Gonelle-Gispert; Leo H. Bühler

    The roles and interactions of platelets and liver sinusoidal endothelial cells in liver regeneration are unclear, and the trigger that initiates hepatocyte proliferation is unknown. We aimed to identify the key factors released by activated platelets that induce liver sinusoidal endothelial cells to produce interleukin-6 (IL-6), a cytokine implicated in the early phase of liver regeneration. We characterized

    更新日期:2020-05-25
  • Pipeline for the Generation and Characterization of Transgenic Human Pluripotent Stem Cells Using the CRISPR/Cas9 Technology
    Cells (IF 5.656) Pub Date : 2020-05-25
    Joffrey Mianné; Chloé Bourguignon; Chloé Nguyen Van; Mathieu Fieldès; Amel Nasri; Said Assou; John De Vos

    Recent advances in genome engineering based on the CRISPR/Cas9 technology have revolutionized our ability to manipulate genomic DNA. Its use in human pluripotent stem cells (hPSCs) has allowed a wide range of mutant cell lines to be obtained at an unprecedented rate. The combination of these two groundbreaking technologies has tremendous potential, from disease modeling to stem cell-based therapies

    更新日期:2020-05-25
  • Metalloproteinases and Their Inhibitors: Potential for the Development of New Therapeutics
    Cells (IF 5.656) Pub Date : 2020-05-25
    Maryam Raeeszadeh-Sarmazdeh; Linh D. Do; Brianne G. Hritz

    The metalloproteinase (MP) family of zinc-dependent proteases, including matrix metalloproteinases (MMPs), a disintegrin and metalloproteases (ADAMs), and a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTSs) plays a crucial role in the extracellular matrix (ECM) remodeling and degradation activities. A wide range of substrates of the MP family includes ECM components, chemokines

    更新日期:2020-05-25
  • Preliminary Trichinella spiralis Infection Ameliorates Subsequent RSV Infection-Induced Inflammatory Response
    Cells (IF 5.656) Pub Date : 2020-05-25
    Ki-Back Chu; Hae-Ahm Lee; Hae-Ji Kang; Eun-Kyung Moon; Fu-Shi Quan

    Respiratory syncytial virus (RSV) infection affects the lives of neonates throughout the globe, causing a high rate of mortality upon hospital admission. Yet, therapeutic options to deal with this pulmonary pathogen are currently limited. Helminth therapy has been well received for its immunomodulatory role in hosts, which are crucial for mitigating a multitude of diseases. Therefore, in this study

    更新日期:2020-05-25
  • Visfatin Increases VEGF-dependent Angiogenesis of Endothelial Progenitor Cells during Osteoarthritis Progression
    Cells (IF 5.656) Pub Date : 2020-05-25
    Chun-Hao Tsai; Shan-Chi Liu; Wen-Hui Chung; Shih-Wei Wang; Min-Huan Wu; Chih-Hsin Tang

    Osteoarthritis (OA) pannus contains a network of neovascularization that is formed and maintained by angiogenesis, which is promoted by vascular endothelial growth factor (VEGF). Bone marrow-derived endothelial progenitor cells (EPCs) are involved in VEGF-induced vessel formation in OA. The adipokine visfatin stimulates the release of inflammatory cytokines during OA progression. In this study, we

    更新日期:2020-05-25
  • In Vitro Modeling of Reoxygenation Effects on mRNA and Protein Levels in Hypoxic Tumor Cells upon Entry into the Bloodstream
    Cells (IF 5.656) Pub Date : 2020-05-25
    Kai Bartkowiak; Claudia Koch; Sebastian Gärtner; Antje Andreas; Tobias M Gorges; Klaus Pantel

    Background: Solid epithelial tumors like breast cancer are the most frequent malignancy in women. Circulating tumor cells (CTCs) are frequently released from hypoxic areas into the blood, where CTCs face elevated oxygen concentrations. This reoxygenation might challenge the use of CTCs for liquid biopsy. Methods: We modeled this situation in vitro using the breast cancer cell lines—MCF-7, MDA-MB-468

    更新日期:2020-05-25
  • TLR2 and Dectin-1 Signaling in Mouse Hematopoietic Stem and Progenitor Cells Impacts the Ability of the Antigen Presenting Cells They Produce to Activate CD4 T Cells
    Cells (IF 5.656) Pub Date : 2020-05-25
    Alba Martínez; Cristina Bono; Daniel Gozalbo; Helen S. Goodridge; M. Luisa Gil; Alberto Yáñez

    Microbial recognition by pattern recognition receptors (PRRs) expressed on hematopoietic stem and progenitor cells (HSPCs) not only activates myelopoiesis but also programs the function of the monocytes and macrophages they produce. For instance, changes in HSPC programming modify the ability of macrophages derived from them to produce inflammatory cytokines. While HSPCs exposed to a TLR2 agonist give

    更新日期:2020-05-25
  • Improving Precise CRISPR Genome Editing by Small Molecules: Is there a Magic Potion?
    Cells (IF 5.656) Pub Date : 2020-05-25
    Nadja Bischoff; Sandra Wimberger; Marcello Maresca; Cord Brakebusch

    Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) genome editing has become a standard method in molecular biology, for the establishment of genetically modified cellular and animal models, for the identification and validation of drug targets in animals, and is heavily tested for use in gene therapy of humans. While the efficiency of CRISPR mediated gene targeting is much higher than

    更新日期:2020-05-25
  • Acute Induction of Translocon-Mediated Ca2+ Leak Protects Cardiomyocytes Against Ischemia/Reperfusion Injury
    Cells (IF 5.656) Pub Date : 2020-05-25
    Ribal Al-Mawla; Mallory Ducrozet; Nolwenn Tessier; Lucille Païta; Bruno Pillot; Yves Gouriou; Camille Villedieu; Zeina Harhous; Alexandre Paccalet; Claire Crola Da Silva; Michel Ovize; Gabriel Bidaux; Sylvie Ducreux; Fabien Van Coppenolle

    During myocardial infarction, dysregulation of Ca2+ homeostasis between the reticulum, mitochondria, and cytosol occurs in cardiomyocytes and leads to cell death. Ca2+ leak channels are thought to be key regulators of the reticular Ca2+ homeostasis and cell survival. The present study aimed to determine whether a particular reticular Ca2+ leak channel, the translocon, also known as translocation channel

    更新日期:2020-05-25
  • Exploring Extracellular Vesicles Biogenesis in Hypothalamic Cells through a Heavy Isotope Pulse/Trace Proteomic Approach
    Cells (IF 5.656) Pub Date : 2020-05-25
    Chee Fan Tan; Hui San Teo; Jung Eun Park; Bamaprasad Dutta; Shun Wilford Tse; Melvin Khee-Shing Leow; Walter Wahli; Siu Kwan Sze

    Studies have shown that the process of extracellular vesicles (EVs) secretion and lysosome status are linked. When the lysosome is under stress, the cells would secrete more EVs to maintain cellular homeostasis. However, the process that governs lysosomal activity and EVs secretion remains poorly defined and we postulated that certain proteins essential for EVs biogenesis are constantly synthesized

    更新日期:2020-05-25
  • Progress and Challenges in The Use of MAP1LC3 as a Legitimate Marker for Measuring Dynamic Autophagy In Vivo
    Cells (IF 5.656) Pub Date : 2020-05-25
    Srinivasa Reddy Bonam; Bayry Jagadeesh; Mario P. Tschan; Sylviane Muller

    Tremendous efforts have been made these last decades to increase our knowledge of intracellular degradative systems, especially in the field of autophagy. The role of autophagy in the maintenance of cell homeostasis is well documented and the existence of defects in the autophagic machinery has been largely described in diseases and aging. Determining the alterations occurring in the many forms of

    更新日期:2020-05-25
  • Lung Tumor Cell-Derived Exosomes Promote M2 Macrophage Polarization
    Cells (IF 5.656) Pub Date : 2020-05-24
    Alexandra Pritchard; Sultan Tousif; Yong Wang; Kenneth Hough; Saad Khan; John Strenkowski; Balu K. Chacko; Victor M. Darley-Usmar; Jessy S. Deshane

    Cellular cross-talk within the tumor microenvironment (TME) by exosomes is known to promote tumor progression. Tumor promoting macrophages with an M2 phenotype are suppressors of anti-tumor immunity. However, the impact of tumor-derived exosomes in modulating macrophage polarization in the lung TME is largely unknown. Herein, we investigated if lung tumor-derived exosomes alter transcriptional and

    更新日期:2020-05-24
  • Epigenetic Features of Human Perinatal Stem Cells Redefine Their Stemness Potential
    Cells (IF 5.656) Pub Date : 2020-05-24
    Giulia Gaggi; Andrea Di Credico; Pascal Izzicupo; Ivana Antonucci; Clara Crescioli; Viviana Di Giacomo; Annalisa Di Ruscio; Giovanni Amabile; Francesco Alviano; Angela Di Baldassarre; Barbara Ghinassi

    Human perinatal stem cells (SCs) can be isolated from fetal annexes without ethical or safety limitations. They are generally considered multipotent; nevertheless, their biological characteristics are still not fully understood. The aim of this study was to investigate the pluripotency potential of human perinatal SCs as compared to human induced pluripotent stem cells (hiPSCs). Despite the low expression

    更新日期:2020-05-24
  • γδ T Cells: The Ideal Tool for Cancer Immunotherapy
    Cells (IF 5.656) Pub Date : 2020-05-24
    Mahboubeh Yazdanifar; Giulia Barbarito; Alice Bertaina; Irma Airoldi

    γδ T cells have recently gained considerable attention as an attractive tool for cancer adoptive immunotherapy due to their potent anti-tumor activity and unique role in immunosurveillance. The remarkable success of engineered T cells for the treatment of hematological malignancies has revolutionized the field of adoptive cell immunotherapy. Accordingly, major efforts are underway to translate this

    更新日期:2020-05-24
  • Lamin A/C Mechanotransduction in Laminopathies
    Cells (IF 5.656) Pub Date : 2020-05-24
    Francesca Donnaloja; Federica Carnevali; Emanuela Jacchetti; Manuela Teresa Raimondi

    Mechanotransduction translates forces into biological responses and regulates cell functionalities. It is implicated in several diseases, including laminopathies which are pathologies associated with mutations in lamins and lamin-associated proteins. These pathologies affect muscle, adipose, bone, nerve, and skin cells and range from muscular dystrophies to accelerated aging. Although the exact mechanisms

    更新日期:2020-05-24
  • Joint Reconstituted Signaling of the IL-6 Receptor via Extracellular Vesicles
    Cells (IF 5.656) Pub Date : 2020-05-24
    Philipp Arnold; Wiebke Lückstädt; Wenjia Li; Inga Boll; Juliane Lokau; Christoph Garbers; Ralph Lucius; Stefan Rose-John; Christoph Becker-Pauly

    Interleukin-6 (IL-6) signaling is a crucial regulatory event important for many biological functions, such as inflammation and tissue regeneration. Accordingly, several pathological conditions are associated with dysregulated IL-6 activity, making it an attractive therapeutic target. For instance, blockade of IL-6 or its α-receptor (IL-6R) by monoclonal antibodies has been successfully used to treat

    更新日期:2020-05-24
  • Molecular Chaperones and Proteolytic Machineries Regulate Protein Homeostasis In Aging Cells
    Cells (IF 5.656) Pub Date : 2020-05-24
    Boris Margulis; Anna Tsimokha; Svetlana Zubova; Irina Guzhova

    Throughout their life cycles, cells are subject to a variety of stresses that lead to a compromise between cell death and survival. Survival is partially provided by the cell proteostasis network, which consists of molecular chaperones, a ubiquitin-proteasome system of degradation and autophagy. The cooperation of these systems impacts the correct function of protein synthesis/modification/transport

    更新日期:2020-05-24
  • Dynamic Characterization of Structural, Molecular, and Electrophysiological Phenotypes of Human-Induced Pluripotent Stem Cell-Derived Cerebral Organoids, and Comparison with Fetal and Adult Gene Profiles
    Cells (IF 5.656) Pub Date : 2020-05-23
    Sarah Logan; Thiago Arzua; Yasheng Yan; Congshan Jiang; Xiaojie Liu; Lai-Kang Yu; Qing-Song Liu; Xiaowen Bai

    Background: The development of 3D cerebral organoid technology using human-induced pluripotent stem cells (iPSCs) provides a promising platform to study how brain diseases are appropriately modeled and treated. So far, understanding of the characteristics of organoids is still in its infancy. The current study profiled, for the first time, the electrophysiological properties of organoids at molecular

    更新日期:2020-05-23
  • The Enigma of Centriole Loss in the 1182-4 Cell Line
    Cells (IF 5.656) Pub Date : 2020-05-23
    Alain Debec; Benjamin Loppin; Chunfeng Zheng; Xiuwen Liu; Timothy L. Megraw

    The Drosophila melanogaster cell line 1182-4, which constitutively lacks centrioles, was established many years ago from haploid embryos laid by females homozygous for the maternal haploid (mh) mutation. This was the first clear example of animal cells regularly dividing in the absence of this organelle. However, the cause of the acentriolar nature of the 1182-4 cell line remained unclear and could

    更新日期:2020-05-23
  • Protein Phase Separation during Stress Adaptation and Cellular Memory
    Cells (IF 5.656) Pub Date : 2020-05-23
    Yasmin Lau; Henry Patrick Oamen; Fabrice Caudron

    Cells need to organise and regulate their biochemical processes both in space and time in order to adapt to their surrounding environment. Spatial organisation of cellular components is facilitated by a complex network of membrane bound organelles. Both the membrane composition and the intra-organellar content of these organelles can be specifically and temporally controlled by imposing gates, much

    更新日期:2020-05-23
  • Computational Tools and Resources Supporting CRISPR-Cas Experiments
    Cells (IF 5.656) Pub Date : 2020-05-22
    Pawel Sledzinski; Mateusz Nowaczyk; Marta Olejniczak

    The CRISPR-Cas system has become a cutting-edge technology that revolutionized genome engineering. The use of Cas9 nuclease is currently the method of choice in most tasks requiring a specific DNA modification. The rapid development in the field of CRISPR-Cas is reflected by the constantly expanding ecosystem of computational tools aimed at facilitating experimental design and result analysis. The

    更新日期:2020-05-22
  • From Tank to Treatment: Modeling Melanoma in Zebrafish
    Cells (IF 5.656) Pub Date : 2020-05-22
    William Tyler Frantz; Craig J Ceol

    Melanoma is the deadliest form of skin cancer and one of few cancers with a growing incidence. A thorough understanding of its pathogenesis is fundamental to developing new strategies to combat mortality and morbidity. Zebrafish—due in large part to their tractable genetics, conserved pathways, and optical properties—have emerged as an excellent system to model melanoma. Zebrafish have been used to

    更新日期:2020-05-22
  • Central Modulation of Selective Sphingosine-1-Phosphate Receptor 1 Ameliorates Experimental Multiple Sclerosis
    Cells (IF 5.656) Pub Date : 2020-05-22
    Alessandra Musella; Antonietta Gentile; Livia Guadalupi; Francesca Romana Rizzo; Francesca De Vito; Diego Fresegna; Antonio Bruno; Ettore Dolcetti; Valentina Vanni; Laura Vitiello; Silvia Bullitta; Krizia Sanna; Silvia Caioli; Sara Balletta; Monica Nencini; Fabio Buttari; Mario Stampanoni Bassi; Diego Centonze; Georgia Mandolesi

    Future treatments of multiple sclerosis (MS), a chronic autoimmune neurodegenerative disease of the central nervous system (CNS), aim for simultaneous early targeting of peripheral immune function and neuroinflammation. Sphingosine-1-phosphate (S1P) receptor modulators are among the most promising drugs with both “immunological” and “non-immunological” actions. Selective S1P receptor modulators have

    更新日期:2020-05-22
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