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Principles of assembly and regulation of condensates of Polycomb repressive complex 1 through phase separation Cell Rep. (IF 8.8) Pub Date : 2024-03-16 Kyle Brown, Pin Yu Chew, Steven Ingersoll, Jorge R. Espinosa, Anne Aguirre, Axel Espinoza, Joey Wen, Kalkidan Astatike, Tatiana G. Kutateladze, Rosana Collepardo-Guevara, Xiaojun Ren
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Ankrd26 is a retinoic acid-responsive plasma membrane-binding and -shaping protein critical for proper cell differentiation Cell Rep. (IF 8.8) Pub Date : 2024-03-16 Anna Sofie Englisch, Sarah Ann Hofbrucker-MacKenzie, Maryam Izadi-Seitz, Michael Manfred Kessels, Britta Qualmann
Morphogens are important triggers for differentiation processes. Yet, downstream effectors that organize cell shape changes in response to morphogenic cues, such as retinoic acid, largely remain elusive. Additionally, derailed plasma membrane-derived signaling often is associated with cancer. We identify Ankrd26 as a critical player in cellular differentiation and as plasma membrane-localized protein
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Age-associated accumulation of B cells promotes macrophage inflammation and inhibits lipolysis in adipose tissue during sepsis Cell Rep. (IF 8.8) Pub Date : 2024-03-15 Anna Carey, Katie Nguyen, Pranathi Kandikonda, Victor Kruglov, Claire Bradley, Korbyn J.V. Dahlquist, Stephanie Cholensky, Whitney Swanson, Vladimir P. Badovinac, Thomas S. Griffith, Christina D. Camell
Non-canonical lipolysis induced by inflammatory cytokines or Toll-like receptor ligands is required for the regulation of inflammation during endotoxemia and sepsis. Canonical lipolysis induced by catecholamines declines during aging due to factors including an expansion of lymphocytes, pro-inflammatory macrophage polarization, and an increase in chronic low-grade inflammation; however, the extent
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Adipose stem cells control obesity-induced T cell infiltration into adipose tissue Cell Rep. (IF 8.8) Pub Date : 2024-03-15 Xiyan Liao, Qin Zeng, Limin Xie, Haowei Zhang, Wanyu Hu, Liuling Xiao, Hui Zhou, Fanqi Wang, Wanqin Xie, Jianfeng Song, Xiaoxiao Sun, Dandan Wang, Yujin Ding, Yayi Jiao, Wuqian Mai, Wufuer Aini, Xiaoyan Hui, Wei Liu, Willa A. Hsueh, Tuo Deng
T cell infiltration into white adipose tissue (WAT) drives obesity-induced adipose inflammation, but the mechanisms of obesity-induced T cell infiltration into WAT remain unclear. Our single-cell RNA sequencing reveals a significant impact of adipose stem cells (ASCs) on T cells. Transplanting ASCs from obese mice into WAT enhances T cell accumulation. C-C motif chemokine ligand 5 (CCL5) is upregulated
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Tonic TCR and IL-1β signaling mediate phenotypic alterations of naive CD4+ T cells Cell Rep. (IF 8.8) Pub Date : 2024-03-15 Takashi Sekiya, Shinya Hidano, Satoshi Takaki
Inert naive CD4 T (T) cells differentiate into functional T helper (Th) or regulatory T (Treg) cell subsets upon encountering antigens, mediating properly directed immune responses. Although all T cells can differentiate into any of the Th and Treg cell subsets, heterogeneity exists among T cells. By constructing reporter mice to detect ongoing T cell receptor (TCR) signaling, we identify that interleukin
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Efficient encoding of aversive location by CA3 long-range projections Cell Rep. (IF 8.8) Pub Date : 2024-03-14 Negar Nikbakht, Martin Pofahl, Albert Miguel-López, Fateme Kamali, Tatjana Tchumatchenko, Heinz Beck
Memorizing locations that are harmful or dangerous is a key capability of all organisms and requires an integration of affective and spatial information. In mammals, the dorsal hippocampus mainly processes spatial information, while the intermediate to ventral hippocampal divisions receive affective information via the amygdala. However, how spatial and aversive information is integrated is currently
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Clec12A, CD301b, and FcγRIIB/III define the heterogeneity of murine DC2s and DC3s Cell Rep. (IF 8.8) Pub Date : 2024-03-14 Lukas Amon, Anna Seichter, Damir Vurnek, Lukas Heger, Lukas Lächele, Nounagnon Romaric Tochoedo, Tomasz Kaszubowski, Lukas Hatscher, Anna Baranska, Giorgi Tchitashvili, Falk Nimmerjahn, Christian Herbert Kurt Lehmann, Diana Dudziak
Over the last decade, multiple studies have investigated the heterogeneity of murine conventional dendritic cells type 2 (cDC2s). However, their phenotypic similarity with monocytes and macrophages renders their clear identification challenging. By creating a protein atlas utilizing multiparameter flow cytometry, we show that ESAM cDC2s are a specialized feature of the spleen strongly differing in
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YTHDF1-CLOCK axis contributes to pathogenesis of allergic airway inflammation through LLPS Cell Rep. (IF 8.8) Pub Date : 2024-03-14 Jing Wang, Yao Zhou, Meng Zhang, Yujiao Wu, Qun Wu, Wen Su, Min Xu, Jinhong Wu, Min Zhang, Jianwei Shuai, Wei Tang, Jiajia Lv, Min Wu, Zhenwei Xia
N6-methyladenosine (m6A) modification has been implicated in many cell processes and diseases. YTHDF1, a translation-facilitating m6A reader, has not been previously shown to be related to allergic airway inflammation. Here, we report that YTHDF1 is highly expressed in allergic airway epithelial cells and asthmatic patients and that it influences proinflammatory responses. CLOCK, a subunit of the circadian
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Wnt5 controls splenic myelopoiesis and neutrophil functional ambivalency during DSS-induced colitis Cell Rep. (IF 8.8) Pub Date : 2024-03-08 Yi Luan, Jiajia Hu, Qijun Wang, Xujun Wang, Wenxue Li, Rihao Qu, Chuan Yang, Barani Kumar Rajendran, Hongyue Zhou, Peng Liu, Ningning Zhang, Yu Shi, Yansheng Liu, Wenwen Tang, Jun Lu, Dianqing Wu
Neutrophils are important innate immune cells with plasticity, heterogenicity, and functional ambivalency. While bone marrow is often regarded as the primary source of neutrophil production, the roles of extramedullary production in regulating neutrophil plasticity and heterogenicity in autoimmune diseases remain poorly understood. Here, we report that the lack of wingless-type MMTV integration site
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Discovery of SQSTM1/p62-dependent P-bodies that regulate the NLRP3 inflammasome Cell Rep. (IF 8.8) Pub Date : 2024-03-07 Elizabeth R. Barrow, Evelina Valionyte, Chris R. Baxter, Yi Yang, Sharon Herath, William A. O’Connell, Justyna Lopatecka, Alexander Strachan, Waldemar Woznica, Holly N. Stephenson, Gyorgy Fejer, Vikram Sharma, Boxun Lu, Shouqing Luo
Autophagy and ribonucleoprotein granules, such as P-bodies (PBs) and stress granules, represent vital stress responses to maintain cellular homeostasis. SQSTM1/p62 phase-separated droplets are known to play critical roles in selective autophagy; however, it is unknown whether p62 can exist as another form in addition to its autophagic droplets. Here, we found that, under stress conditions, including
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Salmonella T3SS-2 virulence enhances gut-luminal colonization by enabling chemotaxis-dependent exploitation of intestinal inflammation Cell Rep. (IF 8.8) Pub Date : 2024-03-07 Ersin Gül, Jemina Huuskonen, Andrew Abi Younes, Luca Maurer, Ursina Enz, Jakob Zimmermann, Mikael E. Sellin, Erik Bakkeren, Wolf-Dietrich Hardt
Typhimurium (.Tm) utilizes the chemotaxis receptor Tsr to exploit gut inflammation. However, the characteristics of this exploitation and the mechanism(s) employed by the pathogen to circumvent antimicrobial effects of inflammation are poorly defined. Here, using different naturally occurring .Tm strains (SL1344 and 14028) and competitive infection experiments, we demonstrate that type-three secretion
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Iron overload in hypothalamic AgRP neurons contributes to obesity and related metabolic disorders Cell Rep. (IF 8.8) Pub Date : 2024-03-07 Yi Zhang, Liwei Chen, Ye Xuan, Lina Zhang, Wen Tian, Yangyang Zhu, Jinghui Wang, Xinyu Wang, Jin Qiu, Jian Yu, Mengyang Tang, Zhen He, Hong Zhang, Si Chen, Yun Shen, Siyi Wang, Rong Zhang, Lingyan Xu, Xinran Ma, Yunfei Liao, Cheng Hu
Iron overload is closely associated with metabolic dysfunction. However, the role of iron in the hypothalamus remains unclear. Here, we find that hypothalamic iron levels are increased, particularly in agouti-related peptide (AgRP)-expressing neurons in high-fat-diet-fed mice. Using pharmacological or genetic approaches, we reduce iron overload in AgRP neurons by central deferoxamine administration
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Bactericidal/permeability-increasing protein instructs dendritic cells to elicit Th22 cell response Cell Rep. (IF 8.8) Pub Date : 2024-03-07 Sigrid Bülow, Katharina U. Ederer, Jonas M. Holzinger, Lisa Zeller, Maren Werner, Martina Toelge, Christina Pfab, Sarah Hirsch, Franziska Göpferich, Andreas Hiergeist, Friederike Berberich-Siebelt, André Gessner
Neutrophil-derived bactericidal/permeability-increasing protein (BPI) is known for its bactericidal activity against gram-negative bacteria and neutralization of lipopolysaccharide. Here, we define BPI as a potent activator of murine dendritic cells (DCs). As shown in GM-CSF-cultured, bone-marrow-derived cells (BMDCs), BPI induces a distinct stimulation profile including IL-2, IL-6, and tumor necrosis
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KIF5B plays important roles in dendritic spine plasticity and dendritic localization of PSD95 and FMRP in the mouse cortex in vivo Cell Rep. (IF 8.8) Pub Date : 2024-03-07 Albert Hiu Ka Fok, Yuhua Huang, Beth Wing Lam So, Qiyu Zheng, Chun Sing Carlos Tse, Xiaoyang Li, Kenneth Kin-Yip Wong, Jiandong Huang, Kwok-On Lai, Cora Sau Wan Lai
Kinesin 1 (KIF5) is one major type of motor protein in neurons, but its members’ function in the intact brain remains less studied. Using two-photon imaging, we find that conditional knockout of (KIF5B cKO) in CaMKIIα-Cre-expressing neurons shows heightened turnover and lower stability of dendritic spines in layer 2/3 pyramidal neurons with reduced spine postsynaptic density protein 95 acquisition
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Poxvirus A51R proteins regulate microtubule stability and antagonize a cell-intrinsic antiviral response Cell Rep. (IF 8.8) Pub Date : 2024-03-07 Dahee Seo, Sabrynna Brito Oliveira, Emily A. Rex, Xuecheng Ye, Luke M. Rice, Flávio Guimarães da Fonseca, Don B. Gammon
Numerous viruses alter host microtubule (MT) networks during infection, but how and why they induce these changes is unclear in many cases. We show that the vaccinia virus (VV)-encoded A51R protein is a MT-associated protein (MAP) that directly binds MTs and stabilizes them by both promoting their growth and preventing their depolymerization. Furthermore, we demonstrate that A51R-MT interactions are
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Integrated stress response signaling acts as a metabolic sensor in fat tissues to regulate oocyte maturation and ovulation Cell Rep. (IF 8.8) Pub Date : 2024-03-07 Lydia Grmai, Manuel Michaca, Emily Lackner, Narayanan Nampoothiri V.P., Deepika Vasudevan
Reproduction is an energy-intensive process requiring systemic coordination. However, the inter-organ signaling mechanisms that relay nutrient status to modulate reproductive output are poorly understood. Here, we use as a model to establish the integrated stress response (ISR) transcription factor, Atf4, as a fat tissue metabolic sensor that instructs oogenesis. We demonstrate that Atf4 regulates
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ciRS-7 and miR-7 regulate ischemia-induced neuronal death via glutamatergic signaling Cell Rep. (IF 8.8) Pub Date : 2024-03-05 Flavia Scoyni, Valeriia Sitnikova, Luca Giudice, Paula Korhonen, Davide M. Trevisan, Ana Hernandez de Sande, Mireia Gomez-Budia, Raisa Giniatullina, Irene F. Ugidos, Hiramani Dhungana, Cristiana Pistono, Nea Korvenlaita, Nelli-Noora Välimäki, Salla M. Kangas, Anniina E. Hiltunen, Emma Gribchenko, Minna U. Kaikkonen-Määttä, Jari Koistinaho, Seppo Ylä-Herttuala, Reetta Hinttala, Morten T. Venø, Junyi
Brain functionality relies on finely tuned regulation of gene expression by networks of non-coding RNAs (ncRNAs) such as the one composed by the circular RNA ciRS-7 (also known as CDR1as), the microRNA miR-7, and the long ncRNA Cyrano. We describe ischemia-induced alterations in the ncRNA network both and and in transgenic mice lacking ciRS-7 or miR-7. Our data show that cortical neurons downregulate
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Exo84c-regulated degradation is involved in the normal self-incompatible response in Brassicaceae Cell Rep. (IF 8.8) Pub Date : 2024-03-04 Tong Zhang, Kun Wang, Shengwei Dou, Erlin Gao, Patrick J. Hussey, Zongcheng Lin, Pengwei Wang
The self-incompatibility system evolves in angiosperms to promote cross-pollination by rejecting self-pollination. Here, we show the involvement of Exo84c in the SI response of both and Arabidopsis. The expression of Exo84c is specifically elevated in stigma during the SI response. Knocking out Exo84c in and SI Arabidopsis partially breaks down the SI response. The SI response inhibits both the protein
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SARS-CoV-2 targets ribosomal RNA biogenesis Cell Rep. (IF 8.8) Pub Date : 2024-02-29 V. Talya Yerlici, Audrey Astori, Nevraj S. Kejiou, Chris A. Jordan, Negin Khosraviani, Janet N.Y. Chan, Razqallah Hakem, Brian Raught, Alexander F. Palazzo, Karim Mekhail
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) hinders host gene expression, curbing defenses and licensing viral protein synthesis and virulence. During SARS-CoV-2 infection, the virulence factor non-structural protein 1 (Nsp1) targets the mRNA entry channel of mature cytoplasmic ribosomes, limiting translation. We show that Nsp1 also restrains translation by targeting nucleolar ribosome
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Integrative systems biology reveals NKG2A-biased immune responses correlate with protection in infectious disease, autoimmune disease, and cancer Cell Rep. (IF 8.8) Pub Date : 2024-02-29 Daniel G. Chen, Jingyi Xie, Jongchan Choi, Rachel H. Ng, Rongyu Zhang, Sarah Li, Rick Edmark, Hong Zheng, Ben Solomon, Katie M. Campbell, Egmidio Medina, Antoni Ribas, Purvesh Khatri, Lewis L. Lanier, Philip J. Mease, Jason D. Goldman, Yapeng Su, James R. Heath
Infection, autoimmunity, and cancer are principal human health challenges of the 21 century. Often regarded as distinct ends of the immunological spectrum, recent studies hint at potential overlap between these diseases. For example, inflammation can be pathogenic in infection and autoimmunity. T resident memory (T) cells can be beneficial in infection and cancer. However, these findings are limited
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Proteomic and metabolomic features in patients with HCC responding to lenvatinib and anti-PD1 therapy Cell Rep. (IF 8.8) Pub Date : 2024-02-27 Zhong-Chen Li, Jie Wang, He-Bin Liu, Yi-Min Zheng, Jian-Hang Huang, Jia-Bin Cai, Lei Zhang, Xin Liu, Ling Du, Xue-Ting Yang, Xiao-Qiang Chai, Ying-Hua Jiang, Zheng-Gang Ren, Jian Zhou, Jia Fan, De-Cai Yu, Hui-Chuan Sun, Cheng Huang, Feng Liu
Combination therapy (lenvatinib/programmed death-1 inhibitor) is effective for treating unresectable hepatocellular carcinoma (uHCC). We reveal that responders have better overall and progression-free survival, as well as high tumor mutation burden and special somatic variants. We analyze the proteome and metabolome of 82 plasma samples from patients with hepatocellular carcinoma (HCC; n = 51) and
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Enhancer-promoter activation by the Kaposi sarcoma-associated herpesvirus episome maintenance protein LANA Cell Rep. (IF 8.8) Pub Date : 2024-02-27 Xiang Ye, Lindsey N. Guerin, Ziche Chen, Suba Rajendren, William Dunker, Yang Zhao, Ruilin Zhang, Emily Hodges, John Karijolich
Higher-order genome structure influences the transcriptional regulation of cellular genes through the juxtaposition of regulatory elements, such as enhancers, close to promoters of target genes. While enhancer activation has emerged as an important facet of Kaposi sarcoma-associated herpesvirus (KSHV) biology, the mechanisms controlling enhancer-target gene expression remain obscure. Here, we discover
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Atypical cofilin signaling drives dendritic cell migration through the extracellular matrix via nuclear deformation Cell Rep. (IF 8.8) Pub Date : 2024-02-27 Harry Warner, Giulia Franciosa, Guus van der Borg, Britt Coenen, Felix Faas, Claire Koenig, Rinse de Boer, René Classens, Sjors Maassen, Maksim V. Baranov, Shweta Mahajan, Deepti Dabral, Frans Bianchi, Niek van Hilten, Herre Jelger Risselada, Wouter H. Roos, Jesper Velgaard Olsen, Laia Querol Cano, Geert van den Bogaart
To mount an adaptive immune response, dendritic cells must migrate to lymph nodes to present antigens to T cells. Critical to 3D migration is the nucleus, which is the size-limiting barrier for migration through the extracellular matrix. Here, we show that inflammatory activation of dendritic cells leads to the nucleus becoming spherically deformed and enables dendritic cells to overcome the typical
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NEMF-mediated Listerin-independent mitochondrial translational surveillance by E3 ligase Pirh2 and mitochondrial protease ClpXP Cell Rep. (IF 8.8) Pub Date : 2024-02-26 Liang Lv, Jinyou Mo, Yumin Qing, Shuchao Wang, Leijie Chen, Anna Mei, Ru Xu, Hualin Huang, Jieqiong Tan, Yifu Li, Jia Liu
The ribosome-associated protein quality control (RQC) pathway acts as a translational surveillance mechanism to maintain proteostasis. In mammalian cells, the cytoplasmic RQC pathway involves nuclear export mediator factor (NEMF)-dependent recruitment of the E3 ligase Listerin to ubiquitinate ribosome-stalled nascent polypeptides on the lysine residue for degradation. However, the quality control of
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Flvcr1a deficiency promotes heme-based energy metabolism dysfunction in skeletal muscle Cell Rep. (IF 8.8) Pub Date : 2024-02-26 Miriam Mistretta, Veronica Fiorito, Anna Lucia Allocco, Giorgia Ammirata, Myriam Y. Hsu, Sabrina Digiovanni, Marzia Belicchi, Laura Napoli, Michela Ripolone, Elena Trombetta, PierLuigi Mauri, Andrea Farini, Mirella Meregalli, Chiara Villa, Paolo Ettore Porporato, Barbara Miniscalco, Simonetta Geninatti Crich, Chiara Riganti, Yvan Torrente, Emanuela Tolosano
The definition of cell metabolic profile is essential to ensure skeletal muscle fiber heterogeneity and to achieve a proper equilibrium between the self-renewal and commitment of satellite stem cells. Heme sustains several biological functions, including processes profoundly implicated with cell metabolism. The skeletal muscle is a significant heme-producing body compartment, but the consequences of
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Tracking the neural codes for words and phrases during semantic composition, working-memory storage, and retrieval Cell Rep. (IF 8.8) Pub Date : 2024-02-26 Théo Desbordes, Jean-Rémi King, Stanislas Dehaene
The ability to compose successive words into a meaningful phrase is a characteristic feature of human cognition, yet its neural mechanisms remain incompletely understood. Here, we analyze the cortical mechanisms of semantic composition using magnetoencephalography (MEG) while participants read one-word, two-word, and five-word noun phrases and compared them with a subsequent image. Decoding of MEG
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Single-cell analysis reveals insights into epithelial abnormalities in ovarian endometriosis Cell Rep. (IF 8.8) Pub Date : 2024-02-26 Jia Yan, Ling Zhou, Mengya Liu, Honglan Zhu, Xin Zhang, E. Cai, Xueqiang Xu, Tinghan Chen, Hongyan Cheng, Jun’e Liu, Shang Wang, Lin Dai, Xiaohong Chang, Fuchou Tang
Ovarian endometriosis is characterized by the growth of endometrial tissue within the ovary, causing infertility and chronic pain. However, its pathophysiology remains unclear. Utilizing high-precision single-cell RNA sequencing, we profile the normal, eutopic, and ectopic endometrium from 34 individuals across proliferative and secretory phases. We observe an increased proportion of ciliated cells
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Roseburia intestinalis sensitizes colorectal cancer to radiotherapy through the butyrate/OR51E1/RALB axis Cell Rep. (IF 8.8) Pub Date : 2024-02-26 Jiali Dong, Bin Wang, Yunong Xiao, Jia Liu, Qi Wang, Huiwen Xiao, Yuxiao Jin, Zhihong Liu, Zhiyuan Chen, Yiliang Li, Saijun Fan, Yuan Li, Ming Cui
The radioresistant signature of colorectal cancer (CRC) hampers the clinical utility of radiotherapy. Here, we find that fecal microbiota transplantation (FMT) potentiates the tumoricidal effects of radiation and degrades the intertwined adverse events in azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced CRC mice. FMT cumulates () in the gastrointestinal tract. Oral gavage of assembles at the
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Metabolic rescue of α-synuclein-induced neurodegeneration through propionate supplementation and intestine-neuron signaling in C. elegans Cell Rep. (IF 8.8) Pub Date : 2024-02-26 Chenyin Wang, Meigui Yang, Dongyao Liu, Chaogu Zheng
Microbial metabolites that can modulate neurodegeneration are promising therapeutic targets. Here, we found that the short-chain fatty acid propionate protects against α-synuclein-induced neuronal death and locomotion defects in a model of Parkinson’s disease (PD) through bidirectional regulation between the intestine and neurons. Both depletion of dietary vitamin B, which induces propionate breakdown
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IL-37 dampens immunosuppressive functions of MDSCs via metabolic reprogramming in the tumor microenvironment Cell Rep. (IF 8.8) Pub Date : 2024-02-26 Yu Mei, Ying Zhu, Kylie Su Mei Yong, Zuhairah Binte Hanafi, Huanle Gong, Yonghao Liu, Huey Yee Teo, Muslima Hussain, Yuan Song, Qingfeng Chen, Haiyan Liu
Interleukin-37 (IL-37) has been shown to inhibit tumor growth in various cancer types. However, the immune regulatory function of IL-37 in the tumor microenvironment is unclear. Here, we established a human leukocyte antigen-I (HLA-I)-matched humanized patient-derived xenograft hepatocellular carcinoma (HCC) model and three murine orthotopic HCC models to study the function of IL-37 in the tumor microenvironment
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The genomic and evolutionary landscapes of anaplastic thyroid carcinoma Cell Rep. (IF 8.8) Pub Date : 2024-02-26 Peter Y.F. Zeng, Stephenie D. Prokopec, Stephen Y. Lai, Nicole Pinto, Michelle A. Chan-Seng-Yue, Roderick Clifton-Bligh, Michelle D. Williams, Christopher J. Howlett, Paul Plantinga, Matthew J. Cecchini, Alfred K. Lam, Iram Siddiqui, Jianxin Wang, Ren X. Sun, John D. Watson, Reju Korah, Tobias Carling, Nishant Agrawal, Nicole Cipriani, Douglas Ball, Barry Nelkin, Lisa M. Rooper, Justin A. Bishop, Cathie
Anaplastic thyroid carcinoma is arguably the most lethal human malignancy. It often co-occurs with differentiated thyroid cancers, yet the molecular origins of its aggressivity are unknown. We sequenced tumor DNA from 329 regions of thyroid cancer, including 213 from patients with primary anaplastic thyroid carcinomas. We also whole genome sequenced 9 patients using multi-region sequencing of both
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ESCRT-I protein UBAP1 controls ventricular expansion and cortical neurogenesis via modulating adherens junctions of radial glial cells Cell Rep. (IF 8.8) Pub Date : 2024-02-24 Danping Lu, Yiqiang Zhi, Huizhen Su, Xiang Lin, Jingjing Lin, Yan Shi, Wenxiang Yi, Chaoyin Hong, Tongtong Zhang, Zhifei Fu, Li-Yu Chen, Zhiqi Zhao, Rong Li, Zhiheng Xu, Wanjin Chen, Ning Wang, Dan Xu
Intricate cerebral cortex formation is orchestrated by the precise behavior and division dynamics of radial glial cells (RGCs). Endocytosis functions in the recycling and remodeling of adherens junctions (AJs) in response to changes in RGC activity and function. Here, we show that conditional disruption of ubiquitin-associated protein 1 (UBAP1), a component of endosomal sorting complex required for
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Akt3 activation by R-Ras in an endothelial cell enforces quiescence and barrier stability of neighboring endothelial cells via Jagged1 Cell Rep. (IF 8.8) Pub Date : 2024-02-24 Jose Luis Herrera, Masanobu Komatsu
Communication between adjacent endothelial cells is important for the homeostasis of blood vessels. We show that quiescent endothelial cells use Jagged1 to instruct neighboring endothelial cells to assume a quiescent phenotype and secure the endothelial barrier. This phenotype enforcement by neighboring cells is operated by R-Ras through activation of Akt3, which results in upregulation of a Notch
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Alternative mRNA splicing events and regulators in epidermal differentiation Cell Rep. (IF 8.8) Pub Date : 2024-02-23 Shota Takashima, Wujianan Sun, Auke B.C. Otten, Pengfei Cai, Shaohong Isaac Peng, Elton Tong, Jolina Bui, McKenzie Mai, Oyumergen Amarbayar, Binbin Cheng, Rowen Jane Odango, Zongkai Li, Kun Qu, Bryan K. Sun
Alternative splicing (AS) of messenger RNAs occurs in ∼95% of multi-exon human genes and generates diverse RNA and protein isoforms. We investigated AS events associated with human epidermal differentiation, a process crucial for skin function. We identified 6,413 AS events, primarily involving cassette exons. We also predicted 34 RNA-binding proteins (RBPs) regulating epidermal AS, including 19 previously
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Engram reactivation mimics cellular signatures of fear Cell Rep. (IF 8.8) Pub Date : 2024-02-23 Rebecca L. Suthard, Ryan A. Senne, Michelle D. Buzharsky, Anh H. Diep, Angela Y. Pyo, Steve Ramirez
Engrams, or the physical substrate of memory, recruit heterogeneous cell types. Targeted reactivation of neurons processing discrete memories drives the behavioral expression of memory, though the underlying landscape of recruited cells and their real-time responses remain elusive. To understand how artificial stimulation of fear affects intra-hippocampal neuron-astrocyte dynamics as well as their
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PRMT1 acts as a suppressor of MHC-I and anti-tumor immunity Cell Rep. (IF 8.8) Pub Date : 2024-02-23 Tirta M. Djajawi, Lizzy Pijpers, Akash Srivaths, David Chisanga, Kok Fei Chan, Simon J. Hogg, Liam Neil, Sarahi Mendoza Rivera, Nenad Bartonicek, Sarah L. Ellis, Terry C.C. Lim Kam Sian, Pouya Faridi, Yang Liao, Bhupinder Pal, Andreas Behren, Wei Shi, Stephin J. Vervoort, Ricky W. Johnstone, Conor J. Kearney
Cancer immunotherapies have demonstrated remarkable success; however, the majority of patients do not respond or develop resistance. Here, we conduct epigenetic gene-targeted CRISPR-Cas9 screens to identify epigenomic factors that limit CD8 T cell-mediated anti-tumor immunity. We identify that PRMT1 suppresses interferon gamma (Ifnγ)-induced MHC-I expression, thus dampening CD8 T cell-mediated killing
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A transcription factor atlas of stem cell fate in planarians Cell Rep. (IF 8.8) Pub Date : 2024-02-23 Hunter O. King, Kwadwo E. Owusu-Boaitey, Christopher T. Fincher, Peter W. Reddien
Whole-body regeneration requires the ability to produce the full repertoire of adult cell types. The planarian contains over 125 cell types, which can be regenerated from a stem cell population called neoblasts. Neoblast fate choice can be regulated by the expression of fate-specific transcription factors (FSTFs). How fate choices are made and distributed across neoblasts versus their post-mitotic
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Working memory features are embedded in hippocampal place fields Cell Rep. (IF 8.8) Pub Date : 2024-02-23 Viktor Varga, Peter Petersen, Ipshita Zutshi, Roman Huszar, Yiyao Zhang, György Buzsáki
Hippocampal principal neurons display both spatial tuning properties and memory features. Whether this distinction corresponds to separate neuron types or a context-dependent continuum has been debated. We report here that the task-context (“splitter”) feature is highly variable along both trial and spatial position axes. Neurons acquire or lose splitter features across trials even when place field
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Serine/arginine-rich splicing factor 7 promotes the type I interferon response by activating Irf7 transcription Cell Rep. (IF 8.8) Pub Date : 2024-02-22 Haley M. Scott, Mackenzie H. Smith, Aja K. Coleman, Kaitlyn S. Armijo, Morgan J. Chapman, Summer L. Apostalo, Allison R. Wagner, Robert O. Watson, Kristin L. Patrick
Tight regulation of macrophage immune gene expression is required to fight infection without risking harmful inflammation. The contribution of RNA-binding proteins (RBPs) to shaping the macrophage response to pathogens remains poorly understood. Transcriptomic analysis reveals that a member of the serine/arginine-rich (SR) family of mRNA processing factors, SRSF7, is required for optimal expression
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Identification of MIMAS, a multifunctional mega-assembly integrating metabolic and respiratory biogenesis factors of mitochondria Cell Rep. (IF 8.8) Pub Date : 2024-02-22 Patrick Horten, Kuo Song, Joshua Garlich, Robert Hardt, Lilia Colina-Tenorio, Susanne E. Horvath, Uwe Schulte, Bernd Fakler, Martin van der Laan, Thomas Becker, Rosemary A. Stuart, Nikolaus Pfanner, Heike Rampelt
The mitochondrial inner membrane plays central roles in bioenergetics and metabolism and contains several established membrane protein complexes. Here, we report the identification of a mega-complex of the inner membrane, termed mitochondrial multifunctional assembly (MIMAS). Its large size of 3 MDa explains why MIMAS has escaped detection in the analysis of mitochondria so far. MIMAS combines proteins
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Poly(ADP-ribosyl)ation of TIMELESS limits DNA replication stress and promotes stalled fork protection Cell Rep. (IF 8.8) Pub Date : 2024-02-22 Julie Rageul, Natalie Lo, Amy L. Phi, Jinal A. Patel, Jennifer J. Park, Hyungjin Kim
Poly(ADP-ribosyl)ation (PARylation), catalyzed mainly by poly(ADP-ribose) polymerase (PARP)1, is a key posttranslational modification involved in DNA replication and repair. Here, we report that TIMELESS (TIM), an essential scaffold of the replisome, is PARylated, which is linked to its proteolysis. TIM PARylation requires recognition of auto-modified PARP1 via two poly(ADP-ribose)-binding motifs,
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The microglial innate immune protein PGLYRP1 mediates neuroinflammation and consequent behavioral changes Cell Rep. (IF 8.8) Pub Date : 2024-02-22 Anup Bhusal, Jae-Hong Kim, Seung-Chan Kim, Eun Mi Hwang, Hoon Ryu, Md. Sekendar Ali, Seung-Chun Park, Won-Ha Lee, Kyoungho Suk
Peptidoglycan recognition protein 1 (PGLYRP1) is a pattern-recognition protein that mediates antibacterial actions and innate immune responses. Its expression and role in neuroinflammatory conditions remain unclear. We observed the upregulation of PGLYRP1 in inflamed human and mouse spinal cord and brain, with microglia being the primary cellular source. Experiments using a recombinant PGLYRP1 protein
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Structural insights into IL-6 signaling inhibition by therapeutic antibodies Cell Rep. (IF 8.8) Pub Date : 2024-02-22 Mingxing Wang, Long Chen, Jin He, Wenqiang Xia, Zihong Ye, Ji She
Antibody inhibitors of the interleukin-6 (IL-6) signaling pathway, such as tocilizumab and sarilumab, have been used to treat rheumatoid arthritis, chimeric antigen receptor T cell-induced cytokine storm, and severe COVID-19 pneumonia. Here, we solve the cryogenic electron microscopy structures of sarilumab and tocilizumab in complex with IL-6R to resolutions of 3.2 and 3.3 Å, respectively. These structures
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Hair cell regeneration, reinnervation, and restoration of hearing thresholds in the avian hearing organ Cell Rep. (IF 8.8) Pub Date : 2024-02-22 Mitsuo P. Sato, Nesrine Benkafadar, Stefan Heller
Hearing starts, at the cellular level, with mechanoelectrical transduction by sensory hair cells. Sound information is then transmitted via afferent synaptic connections with auditory neurons. Frequency information is encoded by the location of hair cells along the cochlear duct. Loss of hair cells, synapses, or auditory neurons leads to permanent hearing loss in mammals. Birds, in contrast, regenerate
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Single-cell spatial transcriptomic and translatomic profiling of dopaminergic neurons in health, aging, and disease Cell Rep. (IF 8.8) Pub Date : 2024-02-21 Peter Kilfeather, Jia Hui Khoo, Katherina Wagner, Han Liang, Maria Claudia Caiazza, Yanru An, Xingju Zhang, Xiaoyan Chen, Natalie Connor-Robson, Zhouchun Shang, Richard Wade-Martins
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Nr5a2 ensures inner cell mass formation in mouse blastocyst Cell Rep. (IF 8.8) Pub Date : 2024-02-21 Yanhua Zhao, Meiting Zhang, Jiqiang Liu, Xinglin Hu, Yuchen Sun, Xingwei Huang, Jingyu Li, Lei Lei
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Perisaccadic and attentional remapping of receptive fields in lateral intraparietal area and frontal eye fields Cell Rep. (IF 8.8) Pub Date : 2024-02-21 Xiao Wang, Cong Zhang, Lin Yang, Min Jin, Michael E. Goldberg, Mingsha Zhang, Ning Qian
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Striatal dopamine release tracks the relationship between actions and their consequences Cell Rep. (IF 8.8) Pub Date : 2024-02-21 G. Hart, T.J. Burton, C.R. Nolan, B.W. Balleine
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YAP mediates apoptosis through failed integrin adhesion reinforcement Cell Rep. (IF 8.8) Pub Date : 2024-02-21 Lidan Shi, Elisabeth Nadjar-Boger, Hamidreza Jafarinia, Aurélie Carlier, Haguy Wolfenson
Extracellular matrix (ECM) rigidity is a major effector of cell fate decisions. Whereas cell proliferation on stiff matrices, wherein Yes-associated protein (YAP) plays a pivotal role, is well documented, activation of apoptosis in response to soft matrices is poorly understood. Here, we show that YAP drives the apoptotic decision as well. We find that in cells on soft matrices, YAP is recruited to
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Kin17 regulates proper cortical localization of Miranda in Drosophila neuroblasts by regulating Flfl expression Cell Rep. (IF 8.8) Pub Date : 2024-02-21 Marisa Connell, Yonggang Xie, Xiaobing Deng, Rui Chen, Sijun Zhu
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Jasmonate signaling pathway confers salt tolerance through a NUCLEAR FACTOR-Y trimeric transcription factor complex in Arabidopsis Cell Rep. (IF 8.8) Pub Date : 2024-02-21 Xing Li, Changjiang Li, Lei Shi, Gaofeng Lv, Xi Li, Yixuan Liu, Xiaojie Jia, Jiyuan Liu, Yuqian Chen, Lei Zhu, Ying Fu
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MIMAS is a new giant multifunctional player in the mitochondrial megacomplex playground Cell Rep. (IF 8.8) Pub Date : 2024-02-21 Kostas Tokatlidis
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Suppression of adaptive NK cell expansion by macrophage-mediated phagocytosis inhibited by 2B4-CD48 Cell Rep. (IF 8.8) Pub Date : 2024-02-21 Rui Li, Cristian Camilo Galindo, Dominique Davidson, Huaijian Guo, Ming-Chao Zhong, Jin Qian, Bin Li, Zsolt Ruzsics, Colleen M. Lau, Timothy E. O'Sullivan, Silvia M. Vidal, Joseph C. Sun, André Veillette
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Histone deacetylase OsHDA706 orchestrates rice broad-spectrum antiviral immunity and is impeded by a viral effector Cell Rep. (IF 8.8) Pub Date : 2024-02-21 Zihang Yang, Juan Du, Xiaoxiang Tan, Hehong Zhang, Lulu Li, Yanjun Li, Zhongyan Wei, Zhongtian Xu, Yuwen Lu, Jianping Chen, Zongtao Sun
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Parallel pathways carrying direction-and orientation-selective retinal signals to layer 4 of the mouse visual cortex Cell Rep. (IF 8.8) Pub Date : 2024-02-21 Helen Wang, Oyshi Dey, Willian N. Lagos, Noor Behnam, Edward M. Callaway, Benjamin K. Stafford
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The CD27/CD70 pathway negatively regulates visceral adipose tissue-resident Th2 cells and controls metabolic homeostasis Cell Rep. (IF 8.8) Pub Date : 2024-02-21 Kevin Englebert, Anaelle Taquin, Abdulkader Azouz, Valérie Acolty, Sylvie Vande Velde, Marie Vanhollebeke, Hadrien Innes, Louis Boon, Tibor Keler, Oberdan Leo, Stanislas Goriely, Muriel Moser, Guillaume Oldenhove
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Complex formation of immunoglobulin superfamily molecules Side-IV and Beat-IIb regulates synaptic specificity Cell Rep. (IF 8.8) Pub Date : 2024-02-20 Jiro Osaka, Arisa Ishii, Xu Wang, Riku Iwanaga, Hinata Kawamura, Shogo Akino, Atsushi Sugie, Satoko Hakeda-Suzuki, Takashi Suzuki
Neurons establish specific synapses based on the adhesive properties of cell-surface proteins while also retaining the ability to form synapses in a relatively non-selective manner. However, comprehensive understanding of the underlying mechanism reconciling these opposing characteristics remains incomplete. Here, we have identified Side-IV/Beat-IIb, members of the immunoglobulin superfamily, as a
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SRRM2 phase separation drives assembly of nuclear speckle subcompartments Cell Rep. (IF 8.8) Pub Date : 2024-02-20 Mengjun Zhang, Zhuang Gu, Shuanghui Guo, Yingtian Sun, Suibin Ma, Shuo Yang, Jierui Guo, Chenxi Fang, Li Shu, Yifan Ge, Zhongwen Chen, Bo Wang
Nuclear speckles (NSs) are nuclear biomolecular condensates that are postulated to form by macromolecular phase separation, although the detailed underlying forces driving NS formation remain elusive. SRRM2 and SON are 2 non-redundant scaffold proteins for NSs. How each individual protein governs assembly of the NS protein network and the functional relationship between SRRM2 and SON are largely unknown
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Systematic evaluation of retroviral LTRs as cis-regulatory elements in mouse embryos Cell Rep. (IF 8.8) Pub Date : 2024-02-20 Jian Yang, Lauryn Cook, Zhiyuan Chen
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The genome of Stephania japonica provides insights into the biosynthesis of cepharanthine Cell Rep. (IF 8.8) Pub Date : 2024-02-20 Zhuo Liu, Shaoqin Shen, Yujie Wang, Shuqi Sun, Tong Yu, Yanhong Fu, Rong Zhou, Chunjin Li, Rui Cao, Yanshu Zhang, Nan Li, Liangdan Sun, Xiaoming Song