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Group I p21-activated kinases in leukemia cell adhesion to fibronectin Cell Adhes. Migr. (IF 3.694) Pub Date : 2021-01-19 Kateřina Kuželová; Adam Obr; Pavla Röselová; Dana Grebeňová; Petra Otevřelová; Barbora Brodská; Aleš Holoubek
ABSTRACT P21-activated kinases (PAK) regulate processes associated with cytoskeleton dynamics. PAK expression in leukemia cells was measured on protein and mRNA levels. In functional assays, we analyzed the effect of PAK inhibitors IPA-3 and FRAX597 on cell adhesivity and viability. PAK2 was dominant in cell lines, whereas primary cells also expressed comparable amount of PAK1 transcription isoforms:
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Keratin 19 maintains E-cadherin localization at the cell surface and stabilizes cell-cell adhesion of MCF7 cells Cell Adhes. Migr. (IF 3.694) Pub Date : 2021-01-04 Sarah Alsharif; Pooja Sharma; Karina Bursch; Rachel Milliken; Van Lam; Arwa Fallatah; Thuc Phan; Meagan Collins; Priya Dohlman; Sarah Tiufekchiev; Georges Nehmetallah; Christopher B. Raub; Byung Min Chung
ABSTRACT A cytoskeletal protein keratin 19 (K19) is highly expressed in breast cancer but its effects on breast cancer cell mechanics are unclear. In MCF7 cells where K19 expression is ablated,we found that K19 is required to maintain rounded epithelial-like shape and tight cell-cell adhesion. A loss of K19 also lowered cell surface E-cadherin levels. Inhibiting internalization restored cell-cell adhesion
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MicroRNA-203 inhibits epithelial-mesenchymal transition, migration, and invasion of renal cell carcinoma cells via the inactivation of the PI3K/AKT signaling pathway by inhibiting CAV1 Cell Adhes. Migr. (IF 3.694) Pub Date : 2020-11-22 Ning Han; Hai Li; Hui Wang
ABSTRACT The present study aimed to evaluate the underlying mechanism of microRNA-203 (miR-203) in renal cell carcinoma (RCC) involving the PI3K/AKT signaling pathway. The results revealed downregulated miR-203 and upregulated CAV1 in RCC tissues. Upregulated miR-203 and downregulated CAV1 increased E-cadherin expression and cell apoptosis, decreased β-catenin and N-cadherin expression and cell proliferation
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Short-term ex-vivo exposure to hydrogen sulfide enhances murine hematopoietic stem and progenitor cell migration, homing, and proliferation Cell Adhes. Migr. (IF 3.694) Pub Date : 2020-11-11 Anoushka Khanna; Namita Indracanti; Rina Chakrabarti; Prem Kumar Indraganti
ABSTRACT For successful transplantation of Hematopoietic Stem cells (HSCs), it is quite necessary that efficient homing, engraftment and retention of HSC self-renewal capacity takes place, which is often restricted due to inadequate number of adult HSCs. Here, we report that short-term ex-vivo treatment of mouse bone marrow mononuclear cells (BMMNCs) to Sodium Hydrogen Sulfide (NaHS, hydrogen sulfide-H2S
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The focal adhesion protein Integrin-Linked Kinase (ILK) as an important player in breast cancer pathogenesis Cell Adhes. Migr. (IF 3.694) Pub Date : 2020-10-12 Katerina Tsirtsaki; Vasiliki Gkretsi
ABSTRACT Cell-extracellular matrix interactions, or focal adhesions (FA), are crucial for tissue homeostasis but are also implicated in cancer. Integrin-Linked Kinase (ILK) is an abundantly expressed FA protein involved in multiple signaling pathways. Here, we reviewed the current literature on the role of ILK in breast cancer (BC). Articles included in vitro and in vivo experiments as well as studies
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KLF5 regulates epithelial-mesenchymal transition of liver cancer cells in the context of p53 loss through miR-192 targeting of ZEB2. Cell Adhes. Migr. (IF 3.694) Pub Date : 2020-10-07 Lan Sun,Xiaona Zhou,Yanmeng Li,Wei Chen,Shanna Wu,Bei Zhang,Jingyi Yao,Anjian Xu
ABSTRACT Krüppel-like factor 5 (KLF5) can both promote and suppress cell migration, but the underlying mechanisms have not been elucidated. In this study, we show that the function of KLF5 in epithelial-mesenchymal transition (EMT) and migration of liver cancer cells depends on the status of the cellular tumor antigen p53 (p53). Furthermore, zinc finger E-box-binding homeobox 2 (ZEB2) is the main regulator
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SLPI facilitates cell migration by regulating lamellipodia/ruffles and desmosomes, in which Galectin4 plays an important role Cell Adhes. Migr. (IF 3.694) Pub Date : 2020-10-04 Yusuke Mizutani; Daisuke Omagari; Manabu Hayatsu; Masaaki Nameta; Kazuo Komiyama; Yoshikazu Mikami; Tatsuo Ushiki
ABSTRACT To elucidate the underlying mechanism of secretory leukocyte protease inhibitor (SLPI)-induced cell migration, we compared SLPI-deleted human gingival carcinoma Ca9-22 (ΔSLPI) cells and original (wild-type: wt) Ca9-22 cells using several microscopic imaging methods and gene expression analysis. Our results indicated reduced migration of ΔSLPI cells compared to wtCa9-22 cells. The lamellipodia/dorsal
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Structure, regulatory factors and cancer-related physiological effects of ADAM9. Cell Adhes. Migr. (IF 3.694) Pub Date : 2020-09-12 M A Haoyuan,L I Yanshu
The ADAMs family belongs to the transmembrane protein superfamily of zinc-dependent metalloproteases, which consists of multiple domains. These domains have independent but complementary functions that enable them to participate in multiple biological processes. Among them, ADAM9 can not only participate in the degradation of extracellular matrix as a metalloprotease, but also mediate tumor cell adhesion
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Fibronectin type III domain-containing 4 promotes the migration and differentiation of bovine skeletal muscle-derived satellite cells via focal adhesion kinase. Cell Adhes. Migr. (IF 3.694) Pub Date : 2020-09-04 Zhao Wang,Zhiqi Wang,Yusheng Pang,Huili Tong,Yunqin Yan,Shuang Li,Shufeng Li
FNDC4 is an anti-inflammatory factor that alters the activation state of macrophages; it is used to treat colitis in mice. However, its role in muscle formation and mechanism of function remains unknown. We found that FNDC4 promotes the bovine MDSCs migration and differentiation. Furthermore, we reported that it interacts with integrin β1 (ITGβ1). FAK, mediated by ITGβ1, regulates cell migration. Our
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Fibronectin type III domain-containing 4 promotes the migration and differentiation of bovine skeletal muscle-derived satellite cells via focal adhesion kinase Cell Adhes. Migr. (IF 3.694) Pub Date : 2020-09-03 Zhao Wang; Zhiqi Wang; Yusheng Pang; Huili Tong; Yunqin Yan; Shuang Li; Shufeng Li
FNDC4 is an anti-inflammatory factor that alters the activation state of macrophages; it is used to treat colitis in mice. However, its role in muscle formation and mechanism of function remains unknown. We found that FNDC4 promotes the bovine MDSCs migration and differentiation. Furthermore, we reported that it interacts with integrin β1 (ITGβ1). FAK, mediated by ITGβ1, regulates cell migration. Our
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The functions of kinesin and kinesin-related proteins in eukaryotes. Cell Adhes. Migr. (IF 3.694) Pub Date : 2020-08-25 Iftikhar Ali,Wei-Cai Yang
Kinesins constitute a superfamily of ATP-driven microtubule motor enzymes that convert the chemical energy of ATP hydrolysis into mechanical work along microtubule tracks. Kinesins are found in all eukaryotic organisms and are essential to all eukaryotic cells, involved in diverse cellular functions such as microtubule dynamics and morphogenesis, chromosome segregation, spindle formation and elongation
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The anti-cancer properties of heparin and its derivatives: a review and prospect. Cell Adhes. Migr. (IF 3.694) Pub Date : 2020-06-14 Sai-Nan Ma,Zhi-Xiang Mao,Yang Wu,Ming-Xing Liang,Dan-Dan Wang,Xiu Chen,Ping-An Chang,Wei Zhang,Jin-Hai Tang
ABSTRACT Heparin, including unfractionated heparin (UFH), low-molecular-weight heparin (LMWH) and heparin derivatives, are commonly used in venous thromboembolism treatment and reportedly have beneficial effects on cancer survival. Heparin can affect the proliferation, adhesion, angiogenesis, migration and invasion of cancer cells via multiple mechanisms. The main mechanisms involve inhibition of heparanase
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Hypoxia regulates human mast cell adhesion to fibronectin via the PI3K/AKT signaling pathway. Cell Adhes. Migr. (IF 3.694) Pub Date : 2020-05-19 Joanna Pastwińska,Aurelia Walczak-Drzewiecka,Magdalena Łukasiak,Marcin Ratajewski,Jarosław Dastych
ABSTRACT A decrease in oxygen concentration is a hallmark of inflammatory reactions resulting from infection or homeostasis disorders. Mast cells interact with extracellular matrix and other cells by adhesion receptors. We investigated the effect of hypoxia on integrin-mediated mast cell adhesion to fibronectin. We found that it was mediated by the α5/β1 receptor and that hypoxia significantly upregulated
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Golgi α1,2-mannosidase I induces clustering and compartmentalization of CD147 during epithelial cell migration. Cell Adhes. Migr. (IF 3.694) Pub Date : 2020-05-18 Miguel Gonzalez-Andrades,Supriya S Jalimarada,Maria Rodriguez-Benavente,Marissa N Feeley,Ashley M Woodward,Dina B AbuSamra,Pablo Argüeso
ABSTRACT CD147 is a widely expressed matrix metalloproteinase inducer involved in the regulation of cell migration. The high glycosylation and ability to undergo oligomerization have been linked to CD147 function, yet there is limited understanding on the molecular mechanisms behind these processes. The current study demonstrates that the expression of Golgi α1,2-mannosidase I is key to maintaining
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FGFR1 regulates proliferation and metastasis by targeting CCND1 in FGFR1 amplified lung cancer. Cell Adhes. Migr. (IF 3.694) Pub Date : 2020-05-18 Ying Yang,Tingting Lu,Ziming Li,Shun Lu
ABSTRACT Aims: The analysis of the online databases revealed that CCND1 expression is correlated with poor prognosis in LSCC. We aimed to explore the function of CCND1 in tumor progression in LSCC. Main methods: The expression of mRNA was measured using qRT-PCR. Protein expression was assessed by Western blot. Cell migration and invasion were assessed by transwell assay. Key findings: CCND1 was co-overexpressed with
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Inhibition of DDR1 reduces invasive features of human A375 melanoma, HT29 colon carcinoma and SK-HEP hepatoma cells. Cell Adhes. Migr. (IF 3.694) Pub Date : 2020-03-03 Irene Romayor,Iker Badiola,Elvira Olaso
ABSTRACT DDR1 is a receptor tyrosine kinases for collagen and an adverse prognostic factor in primary and metastatic tumors.Despite this, DDR1 signaling and its functional consequences in tumor development remain unclear. RT-PCR and Western blot show that A375, colon carcinoma HT29 and liver carcinoma SK-HEP human cell lines express functional DDR1 that phosphorylates in response to collagen type I
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EpCAM-claudin-tetraspanin-modulated ovarian cancer progression and drug resistance. Cell Adhes. Migr. (IF 3.694) Pub Date : 2020-02-24 Zehra Tavsan,Hülya Ayar Kayalı
ABSTRACT Alterations of cell adhesion are involved in cancer progression, but the mechanisms underlying the progression and cell adhesion have remained poorly understood. Focusing on the complex between EpCAM, claudins and tetraspanins, we described a sequence of events by which of the molecules associate each other in ovarian cancer. The interactions between molecules were evaluated by immunoprecipitations
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Thymol alleviates AGEs-induced podocyte injury by a pleiotropic effect via NF-κB-mediated by RhoA/ROCK signalling pathway. Cell Adhes. Migr. (IF 3.694) Pub Date : 2020-02-07 Qinglian Wang,Zhenwei Shen,Guanghui Qi,Yanfang Zhao,Hongge Zhang,Rong Wang
ABSTRACT Advanced glycation end products (AGE) are those of the most powerful pathogenic factors that related to diabetic complications. In our study, we investigated the beneficial effects of thymol on AGE induced cell injury and apoptosis in human podocytes (HPCs) and attempted to clarify its mechanisms. Our results revealed that stimulation with AGE could significantly activate RhoA/NF-κB pathway
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BST2 regulates interferon gamma-dependent decrease in invasion of HTR-8/SVneo cells via STAT1 and AKT signaling pathways and expression of E-cadherin. Cell Adhes. Migr. (IF 3.694) Pub Date : 2020-01-20 Sonam Verma,Amandeep Kaur Kang,Rahul Pal,Satish Kumar Gupta
ABSTRACT The mechanism by which interferon-gamma (IFN-γ) downregulates trophoblast invasion needs further investigation. Treatment of HTR-8/SVneo cells with IFN-γ led to a decrease in their invasion concomitant with an increased expression of BST2. Silencing of BST2 by siRNA showed a significant increase in their invasion and spreading after treatment with IFN-γ as well as downregulated expression
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The effect of fluid shear stress on fibroblasts and stem cells on plane and groove topographies. Cell Adhes. Migr. (IF 3.694) Pub Date : 2020-01-16 Xing Lei,Bin Liu,Hao Wu,Xiao Wu,Xiu-Li Wang,Yue Song,Shuai-Shuai Zhang,Jun-Qin Li,Long Bi,Guo-Xian Pei
ABSTRACT In this study, we aimed to study the effect of fluid shear stress on fibroblasts and BMSCs on plane and groove topographies. The results showed that 0.6-Hz stress had the greatest influence on the alignment, polarity, migration and adhesion of fibroblasts on plane by increasing the expression of reoriented actin and vinculin; whereas 1.0-Hz stress promoted differentiation of fibroblasts into
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Effect arylamine N-acetyltransferase 1 on morphology, adhesion, migration, and invasion of MDA-MB-231 cells: role of matrix metalloproteinases and integrin αV. Cell Adhes. Migr. (IF 3.694) Pub Date : 2020-01-07 Pengcheng Li,Neville J Butcher,Rodney F Minchin
ABSTRACT Reducted arylamine N-acetyltransferase (NAT1) in breast cancers is associated with poor patient survival. NAT1 has also been associated with changes in cancer cell survival and invasion both in vitro and in vivo. Here, we report the effects of NAT1 in cancer cell invasion by addressing its role in adherence, migration, and invasion in vitro. The NAT1 gene was deleted in MDA-MB-231, HT-29 and
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Paracrine signalling of AGR2 stimulates RhoA function in fibroblasts and modulates cell elongation and migration. Cell Adhes. Migr. (IF 3.694) Pub Date : 2019-12-01 Hitesh Bhagavanbhai Mangukiya,Hema Negi,Siva Bharath Merugu,Qudsia Sehar,Dhahiri Saidi Mashausi,Fakhar-Un-Nisa Yunus,Zhenghua Wu,Dawei Li
The most prominent cancer-associated fibroblasts (CAFs) in tumor stroma is known to form a protective structure to support tumor growth. Anterior gradient-2 (AGR2), a tumor secretory protein is believed to play a pivotal role during tumor microenvironment (TME) development. Here, we report that extracellular AGR2 enhances fibroblasts elongation and migration significantly. The early stimulation of
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Splicing factor-modulated generation of mechano growth factor regulates physiological processes in osteoblasts under mechanical stimuli. Cell Adhes. Migr. (IF 3.694) Pub Date : 2019-12-01 Qian Yi,Huan Liu,Jianguo Feng,Yanjiao Wu,Weichao Sun,Mengting Ou,Liling Tang
Mechanical stimuli influence various physiological processes in osteoblasts. We previously showed that mechano-growth factor (MGF), a splicing variant of insulin-like growth factor 1, is highly expressed in osteoblasts in response to mechanical stimuli. This study aims to explore the systemic functions of MGF in osteoblasts, and the mechanisms by which mechanical stress regulates the alternative splicing
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Stable contacts of naïve CD4 T cells with migratory dendritic cells are ICAM-1-dependent but dispensable for proliferation in vivo. Cell Adhes. Migr. (IF 3.694) Pub Date : 2019-12-01 Stav Kozlovski,Ofir Atrakchi,Sara W Feigelson,Ziv Shulman,Ronen Alon
It is unclear if naïve T cells require dendritic cell ICAMs to proliferate inside lymph nodes. To check if and when CD4 lymphocytes use ICAMs on migratory DCs, wild-type and ICAM-1 and 2 double knock out bone marrow-derived DCs pulsed with saturating levels of an OT-II transgene-specific ovalbumin-derived peptide were co-transferred into skin-draining lymph nodes. Intravital imaging of OT-II lymphocytes
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Predictable fibroblast tension generation by measuring compaction of anchored collagen matrices using microscopy and optical coherence tomography. Cell Adhes. Migr. (IF 3.694) Pub Date : 2019-12-01 Melville B Vaughan,Gang Xu,Tracy L Morris,Pratiksha Kshetri,Jing X Herwig
The anchored fibroblast-populated collagen matrix (aFPCM) is an appropriate model to study fibrocontractive disease mechanisms. Our goal was to determine if aFPCM height reduction (compaction) during development is sufficient to predict tension generation. Compaction was quantified daily by both traditional light microscopy and an optical coherence tomography (OCT) system. Contraction in aFPCM was
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The effect of cytokines produced by human adipose tissue on monocyte adhesion to the endothelium. Cell Adhes. Migr. (IF 3.694) Pub Date : 2019-12-01 Sona Cejkova,Hana Kubatova,Filip Thieme,Libor Janousek,Jiri Fronek,Rudolf Poledne,Ivana Kralova Lesna
Visceral adipose tissue (VAT) may play a critical role in atherosclerotic cardiovascular disease. The goal of this study was to determine the effect of human VAT-released pro‑inflammatory cytokines on monocyte adhesion to the endothelium. The cytokine effects on monocyte adhesion to the endothelial cells (ECs) were tested using adipose tissue-conditioned media (ATCM) prepared by culturing human VAT
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Caffeine inhibits hypoxia-induced renal fibroblast activation by antioxidant mechanism. Cell Adhes. Migr. (IF 3.694) Pub Date : 2019-12-01 Angkhana Nilnumkhum,Rattiyaporn Kanlaya,Sunisa Yoodee,Visith Thongboonkerd
Caffeine has been demonstrated to possess anti-fibrotic activity against liver fibrosis. However, its role in renal fibrosis remained unclear. This study investigated the effects of caffeine on renal fibroblast activation induced by hypoxia (one of the inducers for renal fibrosis). BHK-21 fibroblasts were cultured under normoxia or hypoxia with or without caffeine treatment. Hypoxia increased levels
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TIMP-2 inhibits metastasis and predicts prognosis of colorectal cancer via regulating MMP-9. Cell Adhes. Migr. (IF 3.694) Pub Date : 2019-12-01 Weimin Wang,Dan Li,Liangliang Xiang,Mengying Lv,Li Tao,Tengyang Ni,Jianliang Deng,Xiancheng Gu,Sunagawa Masatara,Yanqing Liu,Yan Zhou
Colorectal cancer has a common cause of morbidity and mortality. Therefore, it is urgent to detect reliable biomarkers to predict prognosis in CRC. Here, we determined the expression of TIMP-2 and MMP-9 in a CRC tissue microarray by immunohistochemistry. We found that lower TIMP-2 or/and higher MMP-9 expression in cancer tissues was correlated with poorer overall survival (OS). TIMP-2 or MMP-9 expression
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BECN1 promotes the migration of NSCLC cells through regulating the ubiquitination of Vimentin. Cell Adhes. Migr. (IF 3.694) Pub Date : 2019-12-01 Zhujun Cheng,Hongbo Xin,Tianyu Han
BECN1/Beclin1 is one of the key proteins in autophagy regulation. However, the biological functions of BECN1 in non-small cell lung cancer (NSCLC) were obscure. Here, we found that neither BECN1 knockdown nor overexpression affected the proliferation of NSCLC cells. Surprisingly, BECN1 overexpression increased cell migration and knocking down BECN1 significantly reduced the migratory ability of NSCLC
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Snail2 induced E-cadherin suppression and metastasis in lung carcinoma facilitated by G9a and HDACs. Cell Adhes. Migr. (IF 3.694) Pub Date : 2019-12-01 Yue Hu,Yayuan Zheng,Mingrui Dai,Jiaxin Wu,Bin Yu,Haihong Zhang,Wei Kong,Hui Wu,Xianghui Yu
Snail2 is a repressor of E-cadherin during carcinogenesis; however, the specific mechanisms involved in this process remain largely unknown. Here, we determined that Snail2 was highly increased during TGF-β-induced EMT process in lung cells. H3K9 methylation was up-regulated and H3K4/H3K56 acetylation were down-regulated at the E-cadherin promoter. Snail2 interacted with G9a and HDACs to exert suppression
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Downregulation of miR-200c stabilizes XIAP mRNA and contributes to invasion and lung metastasis of bladder cancer. Cell Adhes. Migr. (IF 3.694) Pub Date : 2019-12-01 Honglei Jin,Lei Xue,Lan Mo,Dongyun Zhang,Xirui Guo,Jiheng Xu,Jingxia Li,Minggang Peng,Xuewei Zhao,Minghao Zhong,Dazhong Xu,Xue-Ru Wu,Haishan Huang,Chuanshu Huang
Our previous studies have demonstrated that XIAP promotes bladder cancer metastasis through upregulating RhoGDIβ/MMP-2 pathway. However, the molecular mechanisms leading to the XIAP upregulation was unclear. In current studies, we found that XIAP was overexpressed in human high grade BCs, high metastatic human BCs, and in mouse invasive BCs. Mechanistic studies indicated that XIAP overexpression in
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Sirtuin 3 promotes microglia migration by upregulating CX3CR1. Cell Adhes. Migr. (IF 3.694) Pub Date : 2019-12-01 Runjing Cao,Shiping Li,Junxiang Yin,Li Guo,Jiong Shi
We studied the role of Sirtuin 3 (SIRT3) in microglial cell migration in ischemic stroke. We used a middle cerebral artery occlusion (MCAO) model of focal ischemia. We then applied lentivirus-packaged SIRT3 overexpression and knock down in microglial N9 cells to investigate the underlying mechanism driving microglial cell migration. More microglial cells appeared in the ischemic lesion side after MCAO
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Gradient fluid shear stress regulates migration of osteoclast precursors. Cell Adhes. Migr. (IF 3.694) Pub Date : 2019-12-01 Yan Gao,Taiyang Li,Qing Sun,Bo Huo
Cell migration is highly sensitive to fluid shear stress (FSS) in blood flow or interstitial fluid flow. However, whether the FSS gradient can regulate the migration of cells remains unclear. In this work, we constructed a parallel-plate flow chamber with different FSS gradients and verified the gradient flow field by particle image velocimetry measurements and finite element analyses. We then investigated
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Platelet endothelial aggregation receptor-1 regulates bovine muscle satellite cell migration and differentiation via integrin beta-1 and focal adhesion kinase. Cell Adhes. Migr. (IF 3.694) Pub Date : 2019-12-01 Yusheng Pang,Ziheng Zhang,Zhao Wang,Yuxin Wang,Yunqin Yan,Shufeng Li,Huili Tong
PEAR1 is highly expressed at bovine MDSC differentiation. However, its biological function remains unclear. Western blotting results showed that PEAR1 increased between day 0 and day 2 of cell differentiation and decreased from day 3. Moreover, scratch test showed that wound healing rate increased after PEAR1 overexpression and decreased upon its suppression. Meanwhile, we found that, upon PEAR1 induction
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N3ICD with the transmembrane domain can effectively inhibit EMT by correcting the position of tight/adherens junctions. Cell Adhes. Migr. (IF 3.694) Pub Date : 2019-12-01 Junyu Tan,Xixun Zhang,Wenjun Xiao,Xiong Liu,Chun Li,Yuxian Guo,Wei Xiong,Yaochen Li
EMT allows a polarized epithelium to lose epithelial integrity and acquire mesenchymal characteristics. Previously, we found that overexpression of the intracellular domain of Notch3 (N3ICD) can inhibit EMT in breast cancer cells. In this study, we aimed to elucidate the influence of N3ICD or N3ICD combined with the transmembrane domain (TD+N3ICD) on the expression and distribution of TJs/AJs and polar
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Decreased expression of circ_0020397 in intracranial aneurysms may be contributing to decreased vascular smooth muscle cell proliferation via increased expression of miR-138 and subsequent decreased KDR expression. Cell Adhes. Migr. (IF 3.694) Pub Date : 2019-12-01 Yushe Wang,Yong Wang,Yu Li,Bin Wang,Zhuang Miao,Xianzhi Liu,Yuanyuan Ma
Dysfunction of vascular smooth muscle cells (VSMCs) mediates intracranial aneurysm (IA). KDR is reported to alleviate IA progression via promoting VSMC proliferation, while the upstream regulators are still unclear. Arterial wall tissues at the aneurysm site from 12 patients were obtained. The real-time PCR result indicated that circRNA_0020397 was down-regulated, but miR-138 was up-regulated in artery
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ARHGEF4-mediates the actin cytoskeleton reorganization of hepatic stellate cells in 3-dimensional collagen matrices. Cell Adhes. Migr. (IF 3.694) Pub Date : 2019-03-24 Xiaowei Zhang,Lan Sun,Wei Chen,Shanna Wu,Yanmeng Li,Xiaojin Li,Bei Zhang,Jingyi Yao,Huan Wang,Anjian Xu
The actin cytoskeleton of hepatic stellate cells (HSCs) is reorganized when they are cultured in 3D collagen matrices. Here, we investigated the molecular mechanism of actin cytoskeleton reorganization in HSCs cultured in 3D floating collagen matrices (FCM) compared to those on 2D polystyrene surfaces (PS). First, we found that the generation of dendritic cellular processes was controlled by Rac1.
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T lymphocytes migrate upstream after completing the leukocyte adhesion cascade. Cell Adhes. Migr. (IF 3.694) Pub Date : 2019-03-17 Nicholas R Anderson,Alexander Buffone,Daniel A Hammer
The leukocyte adhesion cascade is of critical importance for both the maintenance of immune homeostasis and the ability of immune cells to perform effector functions. Here, we present data showing CD4+ T cells migrate upstream (against the direction of flow) after completing the leukocyte adhesion cascade on surfaces displaying either ICAM-1 or ICAM-1 and VCAM-1, but migrate downstream on surfaces
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Small G protein signalling modulator 2 (SGSM2) is involved in oestrogen receptor-positive breast cancer metastasis through enhancement of migratory cell adhesion via interaction with E-cadherin. Cell Adhes. Migr. (IF 3.694) Pub Date : 2019-02-11 Juo-Han Lin,Wen-Jui Lee,Han-Chung Wu,Chih-Hsiung Wu,Li-Ching Chen,Chi-Cheng Huang,Hang-Lung Chang,Tzu-Chun Cheng,Hui-Wen Chang,Chi-Tang Ho,Shih-Hsin Tu,Yuan-Soon Ho
The function of small G protein signalling modulators (SGSM1/2/3) in cancer remains unknown. Our findings demonstrated that SGSM2 is a plasma membrane protein that strongly interacted with E-cadherin/β-catenin. SGSM2 downregulation enhanced the phosphorylation of focal adhesion kinase (FAK; Y576/577), decreased the expression of epithelial markers such as E-cadherin, β-catenin, and Paxillin, and increased
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Hyaluronan-CD44 interactions mediate contractility and migration in periodontal ligament cells. Cell Adhes. Migr. (IF 3.694) Pub Date : 2019-02-08 Zeinab Al-Rekabi,Adriane M Fura,Ilsa Juhlin,Alaa Yassin,Tracy E Popowics,Nathan J Sniadecki
The role of hyaluronan (HA) in periodontal healing has been speculated via its interaction with the CD44 receptor. While HA-CD44 interactions have previously been implicated in numerous cell types; effect and mechanism of exogenous HA on periodontal ligament (PDL) cells is less clear. Herein, we examine the effect of exogenous HA on contractility and migration in human and murine PDL cells using arrays
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Fluid shear stress induces cell migration and invasion via activating autophagy in HepG2 cells. Cell Adhes. Migr. (IF 3.694) Pub Date : 2019-01-31 Zhiping Yan,Guanyue Su,Wenbo Gao,Jia He,Yang Shen,Ye Zeng,Xiaoheng Liu
Fluid shear stress (FSS) regulates the metastasis of hepatocellular carcinoma (HCC). In the present study, we aimed to study the role of autophagy in HCC cells under FSS. The results showed that FSS upregulated the protein markers of autophagy, induced LC3B aggregation and formation of autophagosomes. Inhibition of integrin by Cliengitide (Cli) or inhibition of the microfilaments formation both inhibited
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The modulation of actin dynamics via atypical Protein Kinase-C activated Cofilin regulates metastasis of colorectal cancer cells. Cell Adhes. Migr. (IF 3.694) Pub Date : 2018-11-18 S M Anisul Islam,Rekha Patel,Raja Reddy Bommareddy,Khandker Mohammad Khalid,Mildred Acevedo-Duncan
Colorectal cancer (CRC) is the third most common cancer in the United States. The exact mechanism of CRC cells metastasis is poorly understood. Actin polymerization is thought to be an initial step in the cancer cell motility cycle which drives the formation of cell protrusions and defines the direction of migration. Cofilin, a significant actin-regulating molecule, regulates the migration of cancer
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Neutrophils as a source of branched-chain, aromatic and positively charged free amino acids. Cell Adhes. Migr. (IF 3.694) Pub Date : 2018-10-29 Svetlana I Galkina,Natalia V Fedorova,Alexander L Ksenofontov,Vladimir I Stadnichuk,Ludmila A Baratova,Galina F Sud'Ina
Neutrophils release branched-chain (valine, isoleucine, leucine), aromatic (tyrosine, phenylalanine) and positively charged free amino acids (arginine, ornithine, lysine, hydroxylysine, histidine) when adhere and spread onto fibronectin. In the presence of agents that impair cell spreading or adhesion (cytochalasin D, fMLP, nonadhesive substrate), neutrophils release the same amino acids, except for
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Signaling by discoidin domain receptor 1 in cancer metastasis. Cell Adhes. Migr. (IF 3.694) Pub Date : 2018-10-13 Mayur Gadiya,Goutam Chakraborty
Collagen is the most abundant component of tumor extracellular matrix (ECM). ECM collagens are known to directly interact with the tumor cells via cell surface receptor and play crucial role in tumor cell survival and promote tumor progression. Collagen receptor DDR1 is a member of receptor tyrosine kinase (RTK) family with a unique motif in the extracellular domain resembling Dictyostelium discoideum
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Protein kinase C Inhibitors selectively modulate dynamics of cell adhesion molecules and cell death in human colon cancer cells. Cell Adhes. Migr. (IF 3.694) Pub Date : 2018-10-11 Muzaffer Dükel,Zehra Tavsan,Duygu Erdogan,Deniz Erkan Gök,Hulya Ayar Kayali
During development of colon cancer, Protein Kinase Cs (PKCs) are involved in regulation of many genes controlling several cellular mechanisms. Here, we examined the changes in cell adhesion molecules and PKCs for colorectal cancer progression. We identified that PKCs affected expression of EpCAM, claudins, tetraspanins. Treatment with low concentrations of PKC inhibitors resulted in decreased cell
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The role of cellular contact and TGF-beta signaling in the activation of the epithelial mesenchymal transition (EMT). Cell Adhes. Migr. (IF 3.694) Pub Date : 2018-10-08 Kelsey Gasior,Nikki J Wagner,Jhon Cores,Rose Caspar,Alyson Wilson,Sudin Bhattacharya,Marlene L Hauck
The epithelial mesenchymal transition (EMT) is one step in the process through which carcinoma cells metastasize by gaining the cellular mobility associated with mesenchymal cells. This work examines the dual influence of the TGF-β pathway and intercellular contact on the activation of EMT in colon (SW480) and breast (MCF7) carcinoma cells. While the SW480 population revealed an intermediate state
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Continuous, label-free, 96-well-based determination of cell migration using confluence measurement. Cell Adhes. Migr. (IF 3.694) Pub Date : 2018-10-08 Christian Mayr,Marlena Beyreis,Heidemarie Dobias,Martin Gaisberger,Julia Fuchs,Martin Pichler,Markus Ritter,Martin Jakab,Katharina Helm,Daniel Neureiter,Tobias Kiesslich
Cellular migration is essential in diverse physiological and pathophysiological processes. Here, we present a protocol for quantitative analysis of migration using confluence detection allowing continuous, non-endpoint measurement with minimal hands-on time under cell incubator conditions. Applicability was tested using substances which enhance (EGF) or inhibit (cytochalasin D, ouabain) migration.
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Recapitulation of molecular regulators of nuclear motion during cell migration. Cell Adhes. Migr. (IF 3.694) Pub Date : 2018-09-27 Alexandra Sneider,Jungwon Hah,Denis Wirtz,Dong-Hwee Kim
Cell migration is a highly orchestrated cellular event that involves physical interactions of diverse subcellular components. The nucleus as the largest and stiffest organelle in the cell not only maintains genetic functionality, but also actively changes its morphology and translocates through dynamic formation of nucleus-bound contractile stress fibers. Nuclear motion is an active and essential process
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The Netrin-4/Laminin γ1/Neogenin-1 complex mediates migration in SK-N-SH neuroblastoma cells. Cell Adhes. Migr. (IF 3.694) Pub Date : 2018-08-30 Andrea A Villanueva,Sofía Puvogel,Pablo Lois,Ernesto Muñoz-Palma,Manuel Ramírez Orellana,Fabiana Lubieniecki,Fernando Casco Claro,Iván Gallegos,Javier García-Castro,Pilar Sanchez-Gomez,Vicente A Torres,Verónica Palma
Neuroblastoma (NB) is the most common pediatric extracranial solid tumor. It arises during development of the sympathetic nervous system. Netrin-4 (NTN4), a laminin-related protein, has been proposed as a key factor to target NB metastasis, although there is controversy about its function. Here, we show that NTN4 is broadly expressed in tumor, stroma and blood vessels of NB patient samples. Furthermore
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Interfering histone deacetylase 4 inhibits the proliferation of vascular smooth muscle cells via regulating MEG3/miR-125a-5p/IRF1. Cell Adhes. Migr. (IF 3.694) Pub Date : 2018-08-29 Xiangtao Zheng,Ziheng Wu,Ke Xu,Yihui Qiu,Xiang Su,Zhen Zhang,Mengtao Zhou
In this study, we investigated the role ofhistone deacetylase 4 (HDAC4) and MEG3/miR-125a-5p/interferonregulatoryfactor 1 (IRF1) on vascular smooth muscle cell (VSMCs)proliferation. Platelet derived growth factor (PDGF)-BB was used toinduce the proliferation and migration of VSMCs. The expressionsof MEG3, miR-125a-5p, HDAC4 and IRF1in VSMCs were detectedby qRT-PCR and western blot, respectively. ChIP
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Annexin A1 in inflammation and breast cancer: a new axis in the tumor microenvironment. Cell Adhes. Migr. (IF 3.694) Pub Date : 2018-08-21 Leonardo A Moraes,Patrick B Ampomah,Lina H K Lim
Targeting inflammation in cancer has shown promise to improve and complement current therapies. The tumor microenvironment plays an important role in cancer growth and metastasis and -tumor associated macrophages possess pro-tumoral and pro-metastatic properties. Annexin A1 (ANXA1) is an immune-modulating protein with diverse functions in the immune system and in cancer. In breast cancer, high ANXA1
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Role of glycosylation in hypoxia-driven cell migration and invasion. Cell Adhes. Migr. (IF 3.694) Pub Date : 2018-08-19 Cecilia Arriagada,Patricio Silva,Vicente A Torres
Hypoxia, a common condition of the tumor microenvironment, induces changes in the proteome of cancer cells, mainly via HIF-1, a transcription factor conformed by a constitutively expressed β-subunit and an oxygen-regulated α-subunit. In hypoxia, HIF-1α stabilizes, forms the heterodimeric complex with HIF-1β, and binds to Hypoxia Response Elements (HRE), activating gene expression to promote metabolic
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A biophysically-defined hyaluronic acid-based compound accelerates migration and stimulates the production of keratinocyte-derived neuromodulators. Cell Adhes. Migr. (IF 3.694) Pub Date : 2018-08-19 Annalisa La Gatta,Antonella D'Agostino,Chiara Schiraldi,Giuseppe Colella,Nicola Cirillo
Hyaluronic acid (HA) preparations are widely used in clinical practice and recent data suggest that commercially available HA-based compounds promote ulcer re-epithelialization and induce pain relief. However, the pathophysiological basis of these effects remains poorly understood. In the present study, we investigated the biophysical, biomolecular and functional properties of a HA preparation combined
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Quercetin inhibits LPS-induced macrophage migration by suppressing the iNOS/FAK/paxillin pathway and modulating the cytoskeleton. Cell Adhes. Migr. (IF 3.694) Pub Date : 2018-08-01 Shuna Cui,Qingqing Wu,Juan Wang,Min Li,Jing Qian,Shihua Li
The natural flavonoid quercetin has antioxidant, anti-inflammatory, and anticancer effects. We investigated the effect of quercetin on lipopolysaccharide (LPS)-induced macrophage migration. Quercetin significantly attenuated LPS-induced inducible nitric oxide synthase (iNOS)-derived nitric oxide (NO) production in RAW264.7 cells without affecting their viability. Additionally, quercetin altered the
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Behavioral remodeling of normal and cancerous epithelial cell lines with differing invasion potential induced by substrate elastic modulus. Cell Adhes. Migr. (IF 3.694) Pub Date : 2018-07-05 Arian Ansardamavandi,Mohammad Tafazzoli-Shadpour,Mohammad Ali Shokrgozar
The micro-environment of cancer cells in the body is mechanically stiffer than that of normal cells. We cultured three breast cell lines of MCF10A-normal, MCF7-noninvasive, and MDA-MB-231-invasive on PDMS substrates with different elastic moduli and different cellular features were examined.Effects of substrate stiffness on cell behavior were evident among all cell lines. Cancerous cells were more
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Discoidin domain receptors: multitaskers for physiological and pathological processes. Cell Adhes. Migr. (IF 3.694) Pub Date : 2018-07-04 Birgit Leitinger,Frédéric Saltel
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Polarity and morphogenesis of the eye epithelium requires the adhesion junction associated adaptor protein Traf4. Cell Adhes. Migr. (IF 3.694) Pub Date : 2018-07-03 Carrie Lynn Hehr,Rami Halabi,Sarah McFarlane
During development, neuroepithelial progenitors acquire apico-basal polarity and adhere to one another via apically located tight and adherens junction complexes. This polarized neuroepithelium must continue to integrate cells arising through cell divisions and intercalation, and allow for cell movements, at the same time as undergoing morphogenesis. Cell proliferation, migration and intercalation
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DDR1 and DDR2 in skin. Cell Adhes. Migr. (IF 3.694) Pub Date : 2018-06-29 Muriel Cario
DDR1 and DDR2 are expressed in skin but their expression differs according to the skin compartment, epidermis, dermis, hypodermis and to the embryonic origin of the cells. In skin, it seems that during physiological processes such as wound healing or pathological processes such as tumorigenesis or systemic sclerosis development only one of the DDR is dysregulated. Furthermore, the altered DDR in pathological
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Inositol polyphosphate-4-phosphatase type II plays critical roles in the modulation of cadherin-mediated adhesion dynamics of pancreatic ductal adenocarcinomas. Cell Adhes. Migr. (IF 3.694) Pub Date : 2018-06-29 Bin Zhang,Weidong Wang,Chonghui Li,Rong Liu
The inositol polyphosphate-4-phosphatase type II (INPP4B) has been mostly proposed to act as a tumor suppressor whose expression is frequently dysregulated in numerous human cancers. To date, little is unveiled about whether and how INPP4B will exert its tumor suppressive function on the turnover of cadherin-based cell-cell adhesion system in pancreatic ductal adenocarcinomas (PDACs) in vitro. Here