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  • Characterization of t-loop formation by TRF2.
    Nucleus (IF 2.792) Pub Date : 2020-07-14
    Leonid A Timashev,Titia de Lange

    ABSTRACT T-loops are thought to hide telomeres from DNA damage signaling and DSB repair pathways. T-loop formation requires the shelterin component TRF2, which represses ATM signaling and NHEJ. Here we establish that TRF2 alone, in the absence of other shelterin proteins can form t-loops. Mouse and human cells contain two isoforms of TRF2, one of which is uncharacterized. We show that both isoforms

    更新日期:2020-07-15
  • CTCF as a boundary factor for cohesin-mediated loop extrusion: evidence for a multi-step mechanism
    Nucleus (IF 2.792) Pub Date : 2020-07-07
    Anders S. Hansen

    ABSTRACT Mammalian genome structure is closely linked to function. At the scale of kilobases to megabases, CTCF and cohesin organize the genome into chromatin loops. Mechanistically, cohesin is proposed to extrude chromatin loops bidirectionally until it encounters occupied CTCF DNA-binding sites. Curiously, loops form predominantly between CTCF binding sites in a convergent orientation. How CTCF interacts

    更新日期:2020-07-07
  • LINC-complex mediated positioning of the vegetative nucleus is involved in calcium and ROS signaling in Arabidopsis pollen tubes
    Nucleus (IF 2.792) Pub Date : 2020-07-07
    Morgan Moser; Andrew Kirkpatrick; Norman Reid Groves; Iris Meier

    ABSTRACT Nuclear movement and positioning play a role in developmental processes throughout life. Nuclear movement and positioning are mediated primarily by linker of nucleoskeleton and cytoskeleton (LINC) complexes. LINC complexes are comprised of the inner nuclear membrane SUN proteins and the outer nuclear membrane (ONM) KASH proteins. In Arabidopsis pollen tubes, the vegetative nucleus (VN) maintains

    更新日期:2020-07-07
  • SATB1-mediated chromatin landscape in T cells
    Nucleus (IF 2.792) Pub Date : 2020-06-10
    Tomas Zelenka; Charalampos Spilianakis

    ABSTRACT The regulatory circuits that define developmental decisions of thymocytes are still incompletely resolved. SATB1 protein is predominantly expressed at the CD4+CD8+cell stage exerting its broad transcription regulation potential with both activatory and repressive roles. A series of post-translational modifications and the presence of potential SATB1 protein isoforms indicate the complexity

    更新日期:2020-06-10
  • Nuclear filaments: role in chromosomal positioning and gene expression.
    Nucleus (IF 2.792) Pub Date : 2020-05-26
    Manindra Bera,Kaushik Sengupta

    ABSTRACT Nuclear lamins form an elastic meshwork underlying the inner nuclear membrane and provide mechanical rigidity to the nucleus and maintain shape. Lamins also maintain chromosome positioning and play important roles in several nuclear processes like replication, DNA damage repair, transcription, and epigenetic modifications. LMNA mutations affect cardiac tissue, muscle tissues, adipose tissues

    更新日期:2020-05-26
  • Dynamics as a cause for the nanoscale organization of the genome.
    Nucleus (IF 2.792) Pub Date : 2020-05-23
    Roman Barth,Genevieve Fourel,Haitham A Shaban

    Chromatin 'blobs' were recently identified by live super-resolution imaging of labeled nucleosomes as pervasive but fleeting structural entities. However, the mechanisms leading to the formation of these blobs and their functional implications are unknown. We explore here whether causal relationships exist between parameters that characterize the chromatin blob dynamics and structure, by adapting a

    更新日期:2020-05-23
  • Telomere-led meiotic chromosome movements: recent update in structure and function
    Nucleus (IF 2.792) Pub Date : 2020-05-15
    CY Lee; CG Bisig; MN Conrad; Y Ditamo; L Previato de Almeida; ME Dresser; RJ Pezza

    ABSTRACT Telomere-led chromosome movement or Rapid Prophase Movements (RPM) occur in meiotic prophase and is a conserved process required for proper homologous chromosome interactions. This type of active movements originates in the cytoplasm and is translated to the telomeres through the nuclear membrane. We highlight our recent advances in the engine generating RPMs in S. cerevisiae, with special

    更新日期:2020-05-15
  • Are the processes of DNA replication and DNA repair reading a common structural chromatin unit?
    Nucleus (IF 2.792) Pub Date : 2020-04-10
    Stefania Mamberti,M Cristina Cardoso

    Decades of investigation on genomic DNA have brought us deeper insights into its organization within the nucleus and its metabolic mechanisms. This was fueled by the parallel development of experimental techniques and has stimulated model building to simulate genome conformation in agreement with the experimental data. Here, we will discuss our recent discoveries on the chromatin units of DNA replication

    更新日期:2020-04-20
  • Emerging roles of cytoskeletal proteins in regulating gene expression and genome organization during differentiation.
    Nucleus (IF 2.792) Pub Date : 2020-03-25
    Xin Xie,S Raza Mahmood,Tamara Gjorgjieva,Piergiorgio Percipalle

    In the eukaryotic cell nucleus, cytoskeletal proteins are emerging as essential players in nuclear function. In particular, actin regulates chromatin as part of ATP-dependent chromatin remodeling complexes, it modulates transcription and it is incorporated into nascent ribonucleoprotein complexes, accompanying them from the site of transcription to polyribosomes. The nuclear actin pool is undistinguishable

    更新日期:2020-04-20
  • The coordination of nuclear envelope assembly and chromosome segregation in metazoans.
    Nucleus (IF 2.792) Pub Date : 2020-03-25
    Shiwei Liu,David Pellman

    The nuclear envelope (NE) is composed of two lipid bilayer membranes that enclose the eukaryotic genome. In interphase, the NE is perforated by thousands of nuclear pore complexes (NPCs), which allow transport in and out of the nucleus. During mitosis in metazoans, the NE is broken down and then reassembled in a manner that enables proper chromosome segregation and the formation of a single nucleus

    更新日期:2020-04-20
  • Bromodomain AAA+ ATPases get into shape.
    Nucleus (IF 2.792) Pub Date : 2020-03-19
    Magdalena Murawska,Andreas G Ladurner

    Bromodomain AAA+ ATPases (ATPases associated with diverse cellular activities) are emerging as oncogenic proteins and compelling targets for anticancer therapies. However, structural and biochemical insight into these machines is missing. A recent study by Cho et al. reports the first cryo-EM structure of a bromodomain AAA+ ATPase and provides first insights into the functions of this putative histone

    更新日期:2020-04-20
  • The role of phosphorylation in the elasticity of the tethers that connect telomeres of separating anaphase chromosomes.
    Nucleus (IF 2.792) Pub Date : 2020-01-17
    Emma Kite,Arthur Forer

    Elastic tethers, connecting telomeres of all separating anaphase chromosome pairs, lose elasticity when they lengthen during anaphase. Treatment with phosphatase inhibitor CalyculinA causes anaphase chromosomes to move backwards after they reach the poles, suggesting that dephosphorylation causes loss of tether elasticity. We added 50nM CalyculinA to living anaphase crane-fly spermatocytes with different

    更新日期:2020-04-20
  • Hyperosmotic stress: in situ chromatin phase separation.
    Nucleus (IF 2.792) Pub Date : 2020-01-10
    Ada L Olins,Travis J Gould,Logan Boyd,Bettina Sarg,Donald E Olins

    Dehydration of cells by acute hyperosmotic stress has profound effects upon cell structure and function. Interphase chromatin and mitotic chromosomes collapse ("congelation"). HL-60/S4 cells remain ~100% viable for, at least, 1 hour, exhibiting shrinkage to ~2/3 their original volume, when placed in 300mM sucrose in tissue culture medium. Fixed cells were imaged by immunostaining confocal and STED

    更新日期:2020-04-20
  • Processive DNA synthesis is associated with localized decompaction of constitutive heterochromatin at the sites of DNA replication and repair.
    Nucleus (IF 2.792) Pub Date : 2019-12-01
    Vadim O Chagin,Britta Reinhart,Annette Becker,Oliver Mortusewicz,K Laurence Jost,Alexander Rapp,Heinrich Leonhardt,M Cristina Cardoso

    Constitutive heterochromatin is considered as a functionally inert genome compartment, important for its architecture and stability. How such stable structure is maintained is not well understood. Here, we apply four different visualization schemes to label it and investigate its dynamics during DNA replication and repair. We show that replisomes assemble over the heterochromatin in a temporally ordered

    更新日期:2019-11-01
  • ALT control, delete: FANCM as an anti-cancer target in Alternative Lengthening of Telomeres.
    Nucleus (IF 2.792) Pub Date : 2019-12-01
    Julienne J O'Rourke,Rohan Bythell-Douglas,Elyse A Dunn,Andrew J Deans

    Break-induced replication is a specific type of DNA repair that has a co-opted role in telomere extension by telomerase-negative cancer cells. This Alternative Lengthening of Telomeres (or 'ALT') is required for viability in approximately 10% of all carcinomas, but up to 50% of the soft-tissue derived sarcomas. In several recent studies, we and others demonstrate that expression and activity of FANCM

    更新日期:2019-11-01
  • Regulation of inner nuclear membrane associated protein degradation.
    Nucleus (IF 2.792) Pub Date : 2019-12-01
    Bailey Koch,Hong-Guo Yu

    The nucleus is enclosed by a double-membrane structure, the nuclear envelope, which separates the nucleoplasm from the cytoplasm. The outer nuclear membrane is continuous with the endoplasmic reticulum (ER), whereas the inner nuclear membrane (INM) is a specialized compartment with a unique proteome. In order to ensure compartmental homeostasis, INM-associated degradation (INMAD) is required for both

    更新日期:2019-11-01
  • Probing the 3D architecture of the plant nucleus with microscopy approaches: challenges and solutions.
    Nucleus (IF 2.792) Pub Date : 2019-12-01
    Tao Dumur,Susan Duncan,Katja Graumann,Sophie Desset,Ricardo S Randall,Ortrun Mittelsten Scheid,Dimiter Prodanov,Christophe Tatout,Célia Baroux

    The eukaryotic cell nucleus is a central organelle whose architecture determines genome function at multiple levels. Deciphering nuclear organizing principles influencing cellular responses and identity is a timely challenge. Despite many similarities between plant and animal nuclei, plant nuclei present intriguing specificities. Complementary to molecular and biochemical approaches, 3D microscopy

    更新日期:2019-11-01
  • The Wilhelm Bernhard workshop: half a century of collegiality.
    Nucleus (IF 2.792) Pub Date : 2019-12-01
    Thoru Pederson

    更新日期:2019-11-01
  • The cell nucleus. A study in Burgundy.
    Nucleus (IF 2.792) Pub Date : 2019-12-01
    Oleg Demidov,Vasilisa Aksenova,Mary Dasso,Alexei Arnaoutov

    Wilhelm Bernhard's revolutionary microscopy techniques helped him put forward the hypothesis of specialized compartmentalization of the nucleus. He also described for the first time the nuclear bodies and peri-chromatin fibrils, and demonstrated that these granules contain an RNA component. The tradition of biennial workshops, named after this great scientist, continues, and this year it took place

    更新日期:2019-11-01
  • MLKS2 is an ARM domain and F-actin-associated KASH protein that functions in stomatal complex development and meiotic chromosome segregation.
    Nucleus (IF 2.792) Pub Date : 2019-12-01
    Hardeep K Gumber,Joseph F McKenna,Andrea F Tolmie,Alexis M Jalovec,Andre C Kartick,Katja Graumann,Hank W Bass

    The linker of nucleoskeleton and cytoskeleton (LINC) complex is an essential multi-protein structure spanning the eukaryotic nuclear envelope. The LINC complex functions to maintain nuclear architecture, positioning, and mobility, along with specialized functions in meiotic prophase and chromosome segregation. Members of the LINC complex were recently identified in maize, an important scientific and

    更新日期:2019-11-01
  • Editorial for the SEB florence special issue: functional organisation of the nuclear periphery.
    Nucleus (IF 2.792) Pub Date : 2019-12-01
    David E Evans,Katja Graumann,Roland Foisner

    更新日期:2019-11-01
  • Identification of new transmembrane proteins concentrated at the nuclear envelope using organellar proteomics of mesenchymal cells.
    Nucleus (IF 2.792) Pub Date : 2019-12-01
    Li-Chun Cheng,Sabyasachi Baboo,Cory Lindsay,Liza Brusman,Salvador Martinez-Bartolomé,Olga Tapia,Xi Zhang,John R Yates,Larry Gerace

    The double membrane nuclear envelope (NE), which is contiguous with the ER, contains nuclear pore complexes (NPCs) - the channels for nucleocytoplasmic transport, and the nuclear lamina (NL) - a scaffold for NE and chromatin organization. Since numerous human diseases linked to NE proteins occur in mesenchyme-derived cells, we used proteomics to characterize NE and other subcellular fractions isolated

    更新日期:2019-11-01
  • SUN/KASH interactions facilitate force transmission across the nuclear envelope.
    Nucleus (IF 2.792) Pub Date : 2019-12-01
    Hongyan Hao,Daniel A Starr

    LINC complexes (Linker of Nucleoskeleton and Cytoskeleton), consisting of inner nuclear membrane SUN (Sad1, UNC-84) proteins and outer nuclear membrane KASH (Klarsicht, ANC-1, and Syne Homology) proteins, are essential for nuclear positioning, cell migration and chromosome dynamics. To test the in vivo functions of conserved interfaces revealed by crystal structures, Cain et al used a combination of

    更新日期:2019-11-01
  • Plant condensin II is required for the correct spatial relationship between centromeres and rDNA arrays.
    Nucleus (IF 2.792) Pub Date : 2019-12-01
    Takuya Sakamoto,Tomoya Sugiyama,Tomoe Yamashita,Sachihiro Matsunaga

    Plants possess the structural maintenance of chromosome (SMC) protein complexes cohesin, condensin, and SMC5/6, which function in fundamental biological processes such as sister chromatid cohesion, chromosome condensation and segregation, and damaged DNA repair. Recently, increasing evidence in several organisms has suggested that condensin is involved in chromatin organizations during interphase.

    更新日期:2019-11-01
  • Pairing of homologous chromosomes in C. elegans meiosis requires DEB-1 - an orthologue of mammalian vinculin.
    Nucleus (IF 2.792) Pub Date : 2019-12-01
    Jana Rohožková,Lenka Hůlková,Jana Fukalová,Petr Flachs,Pavel Hozák

    During meiosis, homologous chromosomes undergo a dramatic movement in order to correctly align. This is a critical meiotic event but the molecular properties of this 'chromosomal dance' still remainunclear. We identified DEB-1 - an orthologue of mammalian vinculin - as a new component of the mechanistic modules responsible for attaching the chromosomes to the nuclear envelope as apart of the LINC complex

    更新日期:2019-11-01
  • Comprehensive nuclear proteome of Arabidopsis obtained by sequential extraction.
    Nucleus (IF 2.792) Pub Date : 2019-12-01
    Chieko Goto,Shoko Hashizume,Yoichiro Fukao,Ikuko Hara-Nishimura,Kentaro Tamura

    In eukaryotes, the nucleus plays key roles in fundamental cellular processes, including DNA replication, chromatin maintenance, transcription, and translation. To better understand the functional diversity of nuclei, we developed a method for the comprehensive extraction of the nuclear proteome from Arabidopsis. We used a buffer with a high sucrose concentration to purify nuclei and then conducted

    更新日期:2019-11-01
  • Vascular smooth muscle cell loss underpins the accelerated atherosclerosis in Hutchinson-Gilford progeria syndrome.
    Nucleus (IF 2.792) Pub Date : 2019-12-01
    Magda R Hamczyk,Vicente Andrés

    Lamin A, a product of the LMNA gene, is an essential nuclear envelope component in most differentiated cells. Mutations in LMNA have been linked to premature aging disorders, including Hutchinson-Gilford progeria syndrome (HGPS). HGPS is caused by progerin, an aberrant form of lamin A that leads to premature death, typically from the complications of atherosclerotic disease. A key characteristic of

    更新日期:2019-11-01
  • Novel contribution of epigenetic changes to nuclear dynamics.
    Nucleus (IF 2.792) Pub Date : 2019-12-01
    Marcel Dreger,Elena Madrazo,Adam Hurlstone,Javier Redondo-Muñoz

    Migrating cells have to cross many physical barriers and confined in 3D environments. The surrounding environment promotes mechano- and biological signals that orchestrate cellular changes, such as cytoskeletal and adhesion rearrangements and proteolytic digestion. Recent studies provide new insights into how the nucleus must alter its shape, localization and mechanical properties in order to promote

    更新日期:2019-11-01
  • Role of lamins in 3D genome organization and global gene expression.
    Nucleus (IF 2.792) Pub Date : 2019-12-01
    Youngjo Kim,Xiaobin Zheng,Yixian Zheng

    Genome-wide mapping of lamin-B1-genome interactions has shown that gene-poor and transcriptionally inactive genomic regions are associated with the nuclear lamina. Numerous studies have suggested that lamins, the major structural components of the nuclear lamina, play a role in global chromatin organization and gene expression. How lamins could influence the 3D genome organization and transcription

    更新日期:2019-11-01
  • Exonuclease domain mutants of yeast DIS3 display genome instability.
    Nucleus (IF 2.792) Pub Date : 2019-12-01
    Karissa L Milbury,Biplab Paul,Azra Lari,Claire Fowler,Ben Montpetit,Peter C Stirling

    The exosome functions to regulate the cellular transcriptome through RNA biogenesis, surveillance, and decay. Mutations in Dis3, a catalytic subunit of the RNA exosome with separable endonuclease and exonuclease activities, are linked to multiple myeloma. Here we report that a cancer-associated DIS3 allele, dis3E729K, provides evidence for DIS3 functioning in mitotic fidelity in yeast. This dis3E729K

    更新日期:2019-11-01
  • Meiotic chromosome movement: what's lamin got to do with it?
    Nucleus (IF 2.792) Pub Date : 2019-12-01
    Dimitra Paouneskou,Verena Jantsch

    Active meiotic chromosome movements are a universally conserved feature. They occur at the early stages of prophase of the first meiotic division and support the chromosome pairing process by (1) efficiently installing the synaptonemal complex between homologous chromosomes, (2) discouraging inadvertent chromosome interactions and (3) bringing homologous chromosomes into proximity. Chromosome movements

    更新日期:2019-11-01
  • Interplay of lamin A and lamin B LADs on the radial positioning of chromatin.
    Nucleus (IF 2.792) Pub Date : 2019-12-01
    Frida Forsberg,Annaël Brunet,Tharvesh M Liyakat Ali,Philippe Collas

    Immunosuppressive drugs such as cyclosporin A (CsA) can elicit hepatotoxicity by affecting gene expression. Here, we address the link between CsA and large-scale chromatin organization in HepG2 hepatocarcinoma cells. We show the existence of lamina-associated domains (LADs) interacting with lamin A, lamin B, or both. These 'A-B', 'A-only' and 'B-only' LADs display distinct fates after CsA treatment:

    更新日期:2019-11-01
  • Ribosomal RNA genes shape chromatin domains associating with the nucleolus.
    Nucleus (IF 2.792) Pub Date : 2019-03-25
    Ariadna Picart-Picolo,Nathalie Picault,Frédéric Pontvianne

    Genomic interactions can occur in addition to those within chromosome territories and can be organized around nuclear bodies. Several studies revealed how the nucleolus anchors higher order chromatin structures of specific chromosome regions displaying heterochromatic features. In this review, we comment on advances in this emerging field, with a particular focus on a recent study published by Quinodoz

    更新日期:2019-11-01
  • The nuclear envelope in higher plant mitosis and meiosis.
    Nucleus (IF 2.792) Pub Date : 2019-03-17
    Monica Pradillo,David Evans,Katja Graumann

    Mitosis and meiosis in higher plants involve significant reconfiguration of the nuclear envelope and the proteins that interact with it. The dynamic series of events involves a range of interactions, movement, breakdown, and reformation of this complex system. Recently, progress has been made in identifying and characterizing the protein and membrane interactome that performs these complex tasks, including

    更新日期:2019-11-01
  • The karyosphere capsule in Rana temporaria oocytes contains structural and DNA-binding proteins.
    Nucleus (IF 2.792) Pub Date : 2018-10-03
    Nadya Ilicheva,Olga Podgornaya,Dmitry Bogolyubov,Galina Pochukalina

    During the last stages of oogenesis, oocyte chromosomes condense and come close together, forming the so-called karyosphere. Karyosphere formation is accompanied by an essential decrease in transcriptional activity. In the grass frog Rana temporaria, the karyosphere is surrounded by an extrachromosomal capsule that separates the chromosomes from the rest of the nucleoplasm. The karyosphere capsule

    更新日期:2019-11-01
  • Depletion of Nesprin-2 is associated with an embryonic lethal phenotype in mice.
    Nucleus (IF 2.792) Pub Date : 2018-09-18
    Carmen Mroß,Marija Marko,Martina Munck,Gernot Glöckner,Susanne Motameny,Janine Altmüller,Angelika A Noegel,Ludwig Eichinger,Vivek S Peche,Sascha Neumann

    Nesprin-2 is a nuclear envelope component and provides a link between cytoskeletal components of the cytoplasm and the nucleoplasm. Several isoforms are generated from its gene Syne2. Loss of the largest isoform Nesprin-2 Giant in mice is associated with a skin phenotype and altered wound healing, loss of C-terminal isoforms in mice leads to cardiomyopathies and neurological defects. Here we attempted

    更新日期:2019-11-01
  • Nanoparticle mediated delivery and small molecule triggered activation of proteins in the nucleus.
    Nucleus (IF 2.792) Pub Date : 2018-09-16
    Hsin-Yi Chiu,Jack A Bates,Jonas Helma,Hanna Engelke,Hartmann Harz,Thomas Bein,Heinrich Leonhardt

    Protein transfection is a versatile tool to study or manipulate cellular processes and also shows great therapeutic potential. However, the repertoire of cost effective techniques for efficient and minimally cytotoxic delivery remains limited. Mesoporous silica nanoparticles (MSNs) are multifunctional nanocarriers for cellular delivery of a wide range of molecules, they are simple and economical to

    更新日期:2019-11-01
  • DNA entry, exit and second DNA capture by cohesin: insights from biochemical experiments.
    Nucleus (IF 2.792) Pub Date : 2018-09-13
    Yasuto Murayama

    Cohesin is a ring-shaped, multi-subunit ATPase assembly that is fundamental to the spatiotemporal organization of chromosomes. The ring establishes a variety of chromosomal structures including sister chromatid cohesion and chromatin loops. At the core of the ring is a pair of highly conserved SMC (Structural Maintenance of Chromosomes) proteins, which are closed by the flexible kleisin subunit. In

    更新日期:2019-11-01
  • Simultaneous dual-channel imaging to quantify interdependent protein recruitment to laser-induced DNA damage sites.
    Nucleus (IF 2.792) Pub Date : 2018-09-13
    Joachim Garbrecht,Harald Hornegger,Sebastien Herbert,Tanja Kaufmann,Josef Gotzmann,Kareem Elsayad,Dea Slade

    Fluorescence microscopy in combination with the induction of localized DNA damage using focused light beams has played a major role in the study of protein recruitment kinetics to DNA damage sites in recent years. Currently published methods are dedicated to the study of single fluorophore/single protein kinetics. However, these methods may be limited when studying the relative recruitment dynamics

    更新日期:2019-11-01
  • Nonreplicative functions of the origin recognition complex.
    Nucleus (IF 2.792) Pub Date : 2018-09-11
    Varvara V Popova,Alexander V Brechalov,Sofia G Georgieva,Daria V Kopytova

    Origin recognition complex (ORC), a heteromeric six-subunit complex, is the central component of the eukaryotic pre-replication complex. Recent data from yeast, frogs, flies and mammals present compelling evidence that ORC and its individual subunits have nonreplicative functions as well. The majority of these functions, such as heterochromatin formation, chromosome condensation, and segregation are

    更新日期:2019-11-01
  • Cardiolaminopathies from bench to bedside: challenges in clinical decision-making with focus on arrhythmia-related outcomes.
    Nucleus (IF 2.792) Pub Date : 2018-08-23
    Giuseppe Boriani,Elena Biagini,Matteo Ziacchi,Vincenzo Livio Malavasi,Marco Vitolo,Marisa Talarico,Erminio Mauro,Giulia Gorlato,Giovanna Lattanzi

    Lamin A/C gene mutations can be associated with cardiac diseases, usually referred to as 'cardiolaminopathies' characterized by arrhythmic disorders and/or left ventricular or biventricular dysfunction up to an overt picture of heart failure. The phenotypic cardiac manifestations of laminopathies are frequently mixed in complex clinical patterns and specifically may include bradyarrhythmias (sinus

    更新日期:2019-11-01
  • Nucleolin modulates compartmentalization and dynamics of histone 2B-ECFP in the nucleolus.
    Nucleus (IF 2.792) Pub Date : 2018-06-26
    Ayantika Sen Gupta,Gaurav Joshi,Sumit Pawar,Kundan Sengupta

    Eukaryotic cells have 2 to 3 discrete nucleoli required for ribosome synthesis. Nucleoli are phase separated nuclear sub-organelles. Here we examined the role of nuclear Lamins and nucleolar factors in modulating the compartmentalization and dynamics of histone 2B (H2B-ECFP) in the nucleolus. Live imaging and Fluorescence Recovery After Photobleaching (FRAP) of labelled H2B, showed that the depletion

    更新日期:2019-11-01
  • Genomic instability and DNA replication defects in progeroid syndromes.
    Nucleus (IF 2.792) Pub Date : 2018-06-23
    Romina Burla,Mattia La Torre,Chiara Merigliano,Fiammetta Vernì,Isabella Saggio

    Progeroid syndromes induced by mutations in lamin A or in its interactors - named progeroid laminopathies - are model systems for the dissection of the molecular pathways causing physiological and premature aging. A large amount of data, based mainly on the Hutchinson Gilford Progeria syndrome (HGPS), one of the best characterized progeroid laminopathy, has highlighted the role of lamins in multiple

    更新日期:2019-11-01
  • Corrigendum.
    Nucleus (IF 2.792) Pub Date : 2018-05-11

    更新日期:2019-11-01
  • Elevated TGF β2 serum levels in Emery-Dreifuss Muscular Dystrophy: Implications for myocyte and tenocyte differentiation and fibrogenic processes.
    Nucleus (IF 2.792) Pub Date : 2018-04-26
    Pia Bernasconi,Nicola Carboni,Giulia Ricci,Gabriele Siciliano,Luisa Politano,Lorenzo Maggi,Tiziana Mongini,Liliana Vercelli,Carmelo Rodolico,Elena Biagini,Giuseppe Boriani,Lucia Ruggiero,Lucio Santoro,Elisa Schena,Sabino Prencipe,Camilla Evangelisti,Elena Pegoraro,Lucia Morandi,Marta Columbaro,Chiara Lanzuolo,Patrizia Sabatelli,Paola Cavalcante,Cristina Cappelletti,Gisèle Bonne,Antoine Muchir,Giovanna

    Among rare diseases caused by mutations in LMNA gene, Emery-Dreifuss Muscular Dystrophy type 2 and Limb-Girdle muscular Dystrophy 1B are characterized by muscle weakness and wasting, joint contractures, cardiomyopathy with conduction system disorders. Circulating biomarkers for these pathologies have not been identified. Here, we analyzed the secretome of a cohort of patients affected by these muscular

    更新日期:2019-11-01
  • Causes and consequences of genomic instability in laminopathies: Replication stress and interferon response.
    Nucleus (IF 2.792) Pub Date : 2018-04-12
    Simona Graziano,Ray Kreienkamp,Nuria Coll-Bonfill,Susana Gonzalo

    Mammalian nuclei are equipped with a framework of intermediate filaments that function as a karyoskeleton. This nuclear scaffold, formed primarily by lamins (A-type and B-type), maintains the spatial and functional organization of the genome and of sub-nuclear compartments. Over the past decade, a body of evidence has highlighted the significance of these structural nuclear proteins in the maintenance

    更新日期:2019-11-01
  • Clinical aspects of Emery-Dreifuss muscular dystrophy.
    Nucleus (IF 2.792) Pub Date : 2018-04-11
    Agnieszka Madej-Pilarczyk

    Emery-Dreifuss muscular dystrophy (EDMD), clinically characterized by scapulo-humero-peroneal muscle atrophy and weakness, multi-joint contractures with spine rigidity and cardiomyopathy with conduction defects, is associated with structural/functional defect of genes that encode the proteins of nuclear envelope, including lamin A and several lamin-interacting proteins. This paper presents clinical

    更新日期:2019-11-01
  • Mechanotransduction, nuclear architecture and epigenetics in Emery Dreifuss Muscular Dystrophy: tous pour un, un pour tous.
    Nucleus (IF 2.792) Pub Date : 2018-04-06
    Andrea Bianchi,Pierluigi Giuseppe Manti,Federica Lucini,Chiara Lanzuolo

    The alteration of the several roles that Lamin A/C plays in the mammalian cell leads to a broad spectrum of pathologies that - all together - are named laminopathies. Among those, the Emery Dreifuss Muscular Dystrophy (EDMD) is of particular interest as, despite the several known mutations of Lamin A/C, the genotype-phenotype correlation still remains poorly understood; this suggests that the epigenetic

    更新日期:2019-11-01
  • An overview of treatment strategies for Hutchinson-Gilford Progeria syndrome.
    Nucleus (IF 2.792) Pub Date : 2018-04-06
    Karim Harhouri,Diane Frankel,Catherine Bartoli,Patrice Roll,Annachiara De Sandre-Giovannoli,Nicolas Lévy

    Hutchinson-Gilford progeria syndrome (HGPS) is a sporadic, autosomal dominant disorder characterized by premature and accelerated aging symptoms leading to death at the mean age of 14.6 years usually due to cardiovascular complications. HGPS is caused by a de novo point mutation in the LMNA gene encoding the intermediate filament proteins lamins A and C which are structural components of the nuclear

    更新日期:2019-11-01
  • Progerin phosphorylation in interphase is lower and less mechanosensitive than lamin-A,C in iPS-derived mesenchymal stem cells.
    Nucleus (IF 2.792) Pub Date : 2018-04-06
    Sangkyun Cho,Amal Abbas,Jerome Irianto,Irena L Ivanovska,Yuntao Xia,Manu Tewari,Dennis E Discher

    Interphase phosphorylation of lamin-A,C depends dynamically on a cell's microenvironment, including the stiffness of extracellular matrix. However, phosphorylation dynamics is poorly understood for diseased forms such as progerin, a permanently farnesylated mutant of LMNA that accelerates aging of stiff and mechanically stressed tissues. Here, fine-excision alignment mass spectrometry (FEA-MS) is developed

    更新日期:2019-11-01
  • Extra View: Sirt1 Acts As A Gatekeeper Of Replication Initiation To Preserve Genomic Stability.
    Nucleus (IF 2.792) Pub Date : 2018-03-27
    Koichi Utani,Mirit I Aladjem

    Since the discovery of a yeast gene silencing modifier (Silent Information Modifier 2, SIR2) and its role in maintaining genomic stability more than two decades ago, SIR2 homologs (sirtuins) were identified in diverse species. Sirtuins are protein deacetylases that play diverse roles in proper cellular metabolism including cell cycle progression and maintenance of genomic stability. In yeast, SIR2

    更新日期:2019-11-01
  • Lipodystrophic syndromes due to LMNA mutations: recent developments on biomolecular aspects, pathophysiological hypotheses and therapeutic perspectives.
    Nucleus (IF 2.792) Pub Date : 2018-03-27
    Corinne Vigouroux,Anne-Claire Guénantin,Camille Vatier,Emilie Capel,Caroline Le Dour,Pauline Afonso,Guillaume Bidault,Véronique Béréziat,Olivier Lascols,Jacqueline Capeau,Nolwenn Briand,Isabelle Jéru

    Mutations in LMNA, encoding A-type lamins, are responsible for laminopathies including muscular dystrophies, lipodystrophies, and premature ageing syndromes. LMNA mutations have been shown to alter nuclear structure and stiffness, binding to partners at the nuclear envelope or within the nucleoplasm, gene expression and/or prelamin A maturation. LMNA-associated lipodystrophic features, combining generalized

    更新日期:2019-11-01
  • Lipodystrophic laminopathies: Diagnostic clues.
    Nucleus (IF 2.792) Pub Date : 2018-03-21
    Cristina Guillín-Amarelle,Antía Fernández-Pombo,Sofía Sánchez-Iglesias,David Araújo-Vilar

    The nuclear lamina is a complex reticular structure that covers the inner face of the nucleus membrane in metazoan cells. It is mainly formed by intermediate filaments called lamins, and exerts essential functions to maintain the cellular viability. Lamin A/C provides mechanical steadiness to the nucleus and regulates genetic machinery. Laminopathies are tissue-specific or systemic disorders caused

    更新日期:2019-11-01
  • Invertebrate models of lamin diseases.
    Nucleus (IF 2.792) Pub Date : 2018-03-21
    Ryszard Rzepecki,Yosef Gruenbaum

    Lamins are evolutionarily conserved nuclear intermediate filament proteins. They provide structural support for the nucleus and help regulate many other nuclear activities. Mutations in human lamin genes, and especially in the LMNA gene, cause numerous diseases, termed laminopathies, including muscle, cardiac, metabolic, neuronal and early aging diseases. Most laminopathies arise from autosomal dominant

    更新日期:2019-11-01
  • Laminopathy-causing lamin A mutations reconfigure lamina-associated domains and local spatial chromatin conformation.
    Nucleus (IF 2.792) Pub Date : 2018-03-09
    Nolwenn Briand,Philippe Collas

    The nuclear lamina contributes to the regulation of gene expression and to chromatin organization. Mutations in A-type nuclear lamins cause laminopathies, some of which are associated with a loss of heterochromatin at the nuclear periphery. Until recently however, little if any information has been provided on where and how lamin A interacts with the genome and on how disease-causing lamin A mutations

    更新日期:2019-11-01
  • H4K20me2: Orchestrating the recruitment of DNA repair factors in nucleotide excision repair.
    Nucleus (IF 2.792) Pub Date : 2018-02-28
    Shalaka Chitale,Holger Richly

    The integrity of the genome is maintained by specific DNA repair pathways. The main pathway removing DNA lesions induced by exposure to UV light is nucleotide excision repair (NER). The DNA damage response at chromatin is accompanied by the recruitment of DNA repair factors to the lesion site and the deposition of specific histone marks. The function of these histone marks in NER stays for the most

    更新日期:2019-11-01
  • Quality control of mRNAs at the entry of the nuclear pore: Cooperation in a complex molecular system.
    Nucleus (IF 2.792) Pub Date : 2018-02-13
    Mohammad Soheilypour,Mohammad R K Mofrad

    Despite extensive research on how mRNAs are quality controlled prior to export into the cytoplasm, the exact underlying mechanisms are still under debate. Specifically, it is unclear how quality control proteins at the entry of the nuclear pore complex (NPC) distinguish normal and aberrant mRNAs. While some of the involved components are suggested to act as switches and recruit different factors to

    更新日期:2019-11-01
  • Calculation of 3D genome structures for comparison of chromosome conformation capture experiments with microscopy: An evaluation of single-cell Hi-C protocols.
    Nucleus (IF 2.792) Pub Date : 2018-02-13
    David Lando,Tim J Stevens,Srinjan Basu,Ernest D Laue

    Single-cell chromosome conformation capture approaches are revealing the extent of cell-to-cell variability in the organization and packaging of genomes. These single-cell methods, unlike their multi-cell counterparts, allow straightforward computation of realistic chromosome conformations that may be compared and combined with other, independent, techniques to study 3D structure. Here we discuss how

    更新日期:2019-11-01
  • Differential staining of peripheral nuclear chromatin with Acridine orange implies an A-form epichromatin conformation of the DNA.
    Nucleus (IF 2.792) Pub Date : 2018-01-25
    Jekaterina Erenpreisa,Jekabs Krigerts,Kristine Salmina,Turs Selga,Hermanis Sorokins,Talivaldis Freivalds

    The chromatin observed by conventional electron microscopy under the nuclear envelope constitutes a single layer of dense 30-35 nm granules, while ∼30 nm fibrils laterally attached to them, form large patches of lamin-associated domains (LADs). This particular surface "epichromatin" can be discerned by specific (H2A+H2B+DNA) conformational antibody at the inner nuclear envelope and around mitotic chromosomes

    更新日期:2019-11-01
  • Extrusion without a motor: a new take on the loop extrusion model of genome organization.
    Nucleus (IF 2.792) Pub Date : 2018-01-05
    C A Brackley,J Johnson,D Michieletto,A N Morozov,M Nicodemi,P R Cook,D Marenduzzo

    Chromatin loop extrusion is a popular model for the formation of CTCF loops and topological domains. Recent HiC data have revealed a strong bias in favour of a particular arrangement of the CTCF binding motifs that stabilize loops, and extrusion is the only model to date which can explain this. However, the model requires a motor to generate the loops, and although cohesin is a strong candidate for

    更新日期:2019-11-01
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