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Long non-coding RNA HOTAIR knockdown alleviates gouty arthritis through miR-20b upregulation and NLRP3 downregulation Cell cycle (IF 3.699) Pub Date : 2021-01-20 Ya-Fei Liu; Guo-Lan Xing; Zhe Chen; Sheng-Hao Tu
ABSTRACT This study aimed to determine the mechanism underlying the regulation of gout by the HOX transcript antisense RNA (HOTAIR) long non-coding RNA (lncRNA). The expression levels of HOTAIR, miR-20b, and Nlrp3 were estimated by qRT-PCR and western blotting. The methylation level of HOTAIR was detected by methylation-specific PCR. The recruitment of DNA methyltransferase 1 (DNMT1) to the lncRNA
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An improved staining method of cell cycle analysis with Sybr Green I for fungi: Cryptococcus neoformans and Saccharomyces cerevisiae Cell cycle (IF 3.699) Pub Date : 2021-01-19 Zhongkai Gu
ABSTRACT Cryptococcus neoformans is a pathogenic fungus which causes millions of deaths and infections, especially threatening immunocompromised individuals. During the development of new drugs, the ubiquitination has been found to play an important role in the regulation of the virulence and cell cycle of this fungus. Based on this mechanism, ubiquitination-related mutant strains exhibiting cell cycle
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USP1-WDR48 deubiquitinase complex enhances TGF-β induced epithelial–mesenchymal transition of TNBC cells via stabilizing TAK1 Cell cycle (IF 3.699) Pub Date : 2021-01-18 Dianwen Han; Lijuan Wang; Bing Chen; Wenjing Zhao; Yiran Liang; Yaming Li; Hanwen Zhang; Ying Liu; Xiaolong Wang; Tong Chen; Chen Li; Xiaojin Song; Dan Luo; Zheng Li; Qifeng Yang
ABSTRACT Triple-negative breast cancer (TNBC) is the most aggressive histological subtype of breast cancer and is characterized by poor outcomes and a lack of specific-targeted therapies. Transforming growth factor-β (TGF-β) acts as the key cytokine in the epithelial–mesenchymal transition (EMT) and the metastasis of TNBC. However, the regulatory mechanisms of the TGF-β signaling pathway remain largely
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Role of Aurora B and Haspin kinases in the metaphase Topoisomerase II checkpoint Cell cycle (IF 3.699) Pub Date : 2021-01-18 M. Johansson; Y. Azuma; D. J. Clarke
ABSTRACT DNA Topoisomerase II (TopoII) uses ATP hydrolysis to decatenate chromosomes so that sister chromatids can faithfully segregate in mitosis. When the TopoII enzyme cycle stalls due to failed ATP hydrolysis, the onset of anaphase is delayed, presumably to allow extra time for decatenation to be completed. Recent evidence revealed that, unlike the spindle assembly checkpoint, this TopoII checkpoint
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Correction Cell cycle (IF 3.699) Pub Date : 2021-01-18
(2021). Correction. Cell Cycle. Ahead of Print.
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miR-365 inhibits the progression of gallbladder carcinoma and predicts the prognosis of Gallbladder carcinoma patients Cell cycle (IF 3.699) Pub Date : 2021-01-18 Ze-Bin Jiang; Bing-Qiang Ma; Zongfeng Feng; Shao-Guang Liu; Peng Gao; Hui-Ting Yan
ABSTRACT Gallbladder carcinoma (GBC) is one of the most common fatal biliary tract tumors in the world. Its 3-year survival rate is 30% and the recurrence rate remains very high. miR-365 was downregulated in numerous tumors and worked as tumor suppressor gene. However, the role of miR-365 in GBC was unclear. In this study, our results found that the expression of miR-365 in GBC tissues was reduced
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LncRNA MIAT downregulates IL-1β, TNF-ɑ to suppress macrophage inflammation but is suppressed by ATP-induced NLRP3 inflammasome activation Cell cycle (IF 3.699) Pub Date : 2021-01-18 Ziye Wang; Yang Kun; Zhao Lei; Wen Dawei; Pan Lin; Wang Jibo
ABSTRACT Cardiovascular disease (CVD) has been identified as the leading cause of premature deaths in rheumatoid arthritis (RA), accounting for about 40 to 50% of all deaths. Macrophage inflammation is regarded as a key point to link to the two diseases. Recently, long non-coding RNAs (lncRNAs) have acknowledged as a regulator of inflammation significantly. Here, we firstly found that lncRNA myocardial
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LncRNA GAS6-AS2 promotes non-small-cell lung cancer cell proliferation via regulating miR-144-3p/ MAPK6 axis Cell cycle (IF 3.699) Pub Date : 2021-01-18 Guangjie Hou; Junfeng Yang; Jinxing Tang; Yi He
ABSTRACT The function of a new long non-coding RNA GAS6-AS2 in non-small cell lung cancer (NSCLC) is not fully understood. In this study, GAS6-AS2 was identified, and its roles as well as mechanisms in regulating proliferation of NSCLCs cells were investigated. qRT-PCR was used to analyze GAS6-AS2, miR-144-3p, and MAPK6 expression. Protein expression was detected by Western blotting. Cell Counting
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HOXC-AS2 mediates the proliferation, apoptosis, and migration of non-small cell lung cancer by combining with HOXC13 gene Cell cycle (IF 3.699) Pub Date : 2021-01-11 Bin Liu; Jing Li; Ji-Man Li; Guang-Yuan Liu; Yong-Sheng Wang
ABSTRACT Non-small cell lung cancer (NSCLC) is the highest incidence and mortality of malignant tumors worldwide and has become a global public health problem. Long non-coding RNAs (LncRNAs) are expected to participate in the progression of NSCLC. This study aims to explore the effects and underlying mechanisms of LncRNA HOXC-AS2 on NSCLC cell proliferation, apoptosis, and migration. The Cell Counting
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Expression of miRNA-203 and its target gene in hair follicle cycle development of Cashmere goat Cell cycle (IF 3.699) Pub Date : 2021-01-11 Tao Ma; Jianyu Li; Jianping Li; Sufang Wu; Xiangba; Huaizhi Jiang; Qialling Zhang
ABSTRACT MicroRNA plays an important regulatory role in the development of all organisms, including hair follicle development. In order to improve domestic cashmere yield, the role of miRNA in hair follicle cycle has become a research hotspot. However, the molecular and cellular mechanisms by which miRNA-203 regulates hair follicle development are not completely understood. In this study, we found
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Circ-FOXM1 knockdown suppresses non-small cell lung cancer development by regulating the miR-149-5p/ATG5 axis Cell cycle (IF 3.699) Pub Date : 2021-01-08 Haitao Wei; Li Li; Haifeng Zhang; Feng Xu; Longqi Chen; Guowei Che; Yun Wang
ABSTRACT Circular RNAs (circRNAs) have been reported to be related to the development of human cancers. However, the function of circ-FOXM1 in non-small cell lung cancer (NSCLC) was largely unknown. Here, we revealed the role and functional mechanism of circ-FOXM1 in NSCLC progression. The relative expression of circ-FOXM1, microRNA-149-5p (miR-149-5p), and autophagy-related 5 (ATG5) was determined
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Silenced lncRNA H19 and up-regulated microRNA-129 accelerates viability and restrains apoptosis of PC12 cells induced by Aβ25-35 in a cellular model of Alzheimer’s disease Cell cycle (IF 3.699) Pub Date : 2021-01-07 Yan-Yun Zhang; Hai-Lan Bao; Li-Xia Dong; Yu Liu; Guo-Wei Zhang; Feng-Mao An
ABSTRACT Accumulating data manifest that long non-coding RNA (lncRNAs) are involved in all kinds of neurodegenerative disorders, consisting of the onset and progression of Alzheimer’s disease (AD). The study was for the research of the mechanism of lncRNA H19 (H19) in viability and apoptosis of PC12 cells induced by Aβ25-35 in a cellular model of AD with the regulation of microRNA (miR)-129 and high
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Over-expression of MEG3 promotes differentiation of bone marrow mesenchymal stem cells into chondrocytes by regulating miR-129-5p/RUNX1 axis Cell cycle (IF 3.699) Pub Date : 2021-01-07 Jun Zhu; Qiwei Fu; Jiahua Shao; Jinhui Peng; Qirong Qian; Yiqin Zhou; Yi Chen
ABSTRACT This study explored the role of MEG3 in the cartilage differentiation of bone marrow mesenchymal stem cells (BMSCs). We investigated the effects of over-expression and knockdown of MEG3 on cell viability, cell differentiation, and the expressions of MEG3, miR-129-5p, COL2, chondrocyte differentiation-related genes (sry-type high-mobility-group box 9 (SOX9), SOX5, Aggrecan, silent information
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DNA-PKcs inhibition impairs HDAC6-mediated HSP90 chaperone function on Aurora A and enhances HDACs inhibitor-induced cell killing by increasing mitotic aberrant spindle assembly Cell cycle (IF 3.699) Pub Date : 2021-01-06 Lan Yu; Yue Lang; Jiaming Guo; Jianming Cai; Zeng-Fu Shang; Benjamin P.C Chen
ABSTRACT Combining targeted therapeutic agents is an attractive cancer treatment strategy associated with high efficacy and low toxicity. DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is an essential factor in DNA damage repair. Studies from us and others have revealed that DNA-PKcs also plays an important role in normal mitosis progression. Histone deacetylase (HDACs) inhibitors commonly
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RNAi-mediated knockdown of PFK1 decreases the invasive capability and metastasis of nasopharyngeal carcinoma cell line, CNE-2 Cell cycle (IF 3.699) Pub Date : 2021-01-06 Shuo Li; Peng He; Zhiwei Wang; Meng Liang; Wei Liao; Yili Huang; Mengshi Chi; Fei Liu; Nan Zen; Rongfei Su; Shulin Chen; Zhigang Liu; Haiyu Hong
ABSTRACT Nasopharyngeal carcinoma (NPC) is the most prevailing malignancy of the head and neck with unique geographic distribution. Southern China has one of the highest incidence rates of NPC in the world. Although radiotherapy and chemotherapy are the most important treatment modalities for NPC, recurrence, and metastasis severely interfere with the survival quality of patients. It is much-needed
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Connecting cell polarity signals to the cytokinetic machinery in yeast and metazoan cells Cell cycle (IF 3.699) Pub Date : 2021-01-05 Joseph O. Magliozzi; James B. Moseley
ABSTRACT Polarized growth and cytokinesis are two fundamental cellular processes that exist in virtually all cell types. Mechanisms for asymmetric distribution of materials allow for cells to grow in a polarized manner. This gives rise to a variety of cell shapes seen throughout all cell types. Following polarized growth during interphase, dividing cells assemble a cytokinetic ring containing the protein
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WHAMM is essential for spindle formation and spindle actin polymerization in maturing mouse oocytes Cell cycle (IF 3.699) Pub Date : 2021-01-05 Yu-Jin Jo; Jeongwoo Kwon; Zhe-Long Jin; Suk Namgoong; Taeho Kwon; Seung-Bin Yoon; Dong-Ho Lee; Ji-Su Kim; Nam-Hyung Kim
ABSTRACT WHAMM (WAS Protein Homolog Associated with Actin, Golgi Membranes, and Microtubules) is involved in Golgi membrane association, microtubule binding, and actin nucleation as a nucleation-promoting factor, which activates the actin-related protein 2/3 complex (the Arp2/3 complex). However, the role of WHAMM in mammalian oocyte maturation is poorly understood. The presence of WHAMM mRNA and protein
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Coronavirus disease 2019 (COVID-19), human erythrocytes and the PKC-alpha/-beta inhibitor chelerythrine –possible therapeutic implication Cell cycle (IF 3.699) Pub Date : 2020-12-11 Mehrdad Ghashghaeinia; Peter Dreischer; Thomas Wieder; Martin Köberle
ABSTRACT The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes COVID-19. Until now, diverse drugs have been used for the treatment of COVID-19. These drugs are associated with severe side effects, e.g. induction of erythrocyte death, named eryptosis. This massively affects the oxygen (O2) supply of the organism. Therefore, three elementary aspects should be considered simultaneously:
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Diminution of microRNA-98 alleviates renal fibrosis in diabetic nephropathy by elevating Nedd4L and inactivating TGF-β/Smad2/3 pathway Cell cycle (IF 3.699) Pub Date : 2020-12-14 Yi Zeng; Zhijian Feng; Yunjuan Liao; Ming Yang; Yihua Bai; Zhenkun He
ABSTRACT MicroRNAs (miRNAs) have already been documented to function in diabetic nephropathy (DN), yet little research has focused on the role of miR-98 in this disease. Here, we discuss the mechanism of miR-98 on the renal fibrosis in DN. Recombinant adeno-associated virus carrying miR-98 inhibitor or Nedd4L overexpression plasmid was injected into DN modeled rats to explore their roles in DN. Renal
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Autophosphorylation-induced self-assembly and STIL-dependent reinforcement underlie Plk4’s ring-to-dot localization conversion around a human centriole Cell cycle (IF 3.699) Pub Date : 2020-12-15 Jung-Eun Park; Lingjun Meng; Eun Kyoung Ryu; Kunio Nagashima; Ulrich Baxa; Jeong Kyu Bang; Kyung S. Lee
ABSTRACT Polo-like kinase 4 (Plk4) is a key regulator of centriole biogenesis. Studies have shown that Plk4 undergoes dynamic relocalization from a ring-like pattern around a centriole to a dot-like morphology at the procentriole assembly site and this event is central for inducing centriole biogenesis. However, the detailed mechanisms underlying Plk4’s capacity to drive its symmetry-breaking ring-to-dot
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Phase separation of the Cep63•Cep152 complex underlies the formation of dynamic supramolecular self-assemblies at human centrosomes Cell cycle (IF 3.699) Pub Date : 2020-11-18 Jong Il Ahn; Jung-Eun Park; Lingjun Meng; Liang Zhang; Tae-Sung Kim; Michael J. Kruhlak; Bo Yeon Kim; Kyung S. Lee
ABSTRACT The centrosome is a unique membraneless organelle that plays a pivotal role in the orderly progression of the cell cycle in animal cells. It has been shown that two pericentriolar scaffold proteins, Cep63 and Cep152, generate a heterotetrameric complex to self-assemble into a higher-order cylindrical architecture around a centriole. However, the mechanisms underlying how they reach their threshold
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FAIM-S functions as a negative regulator of NF-κB pathway and blocks cell cycle progression in NSCLC cells Cell cycle (IF 3.699) Pub Date : 2020-11-29 Pengcheng Wang; Wenze Xun; Tianyu Han; Zhujun Cheng
ABSTRACT Tumorigenesis is closely related to the disorder of the cell cycle. The cell cycle progression includes the interphase (G0/G1, S, and G2 phase) and mitosis (M phase). CCND1 is a key protein that regulates the entry of the G0/G1 phase into the S phase. In our study, we found that the short form of Fas Apoptosis Inhibitory Molecule 1 (FAIM-S) could regulate the expression of CCND1 and had a
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Suppression of store-operated Ca2+ entry regulated by silencing Orai1 inhibits C6 glioma cell motility via decreasing Pyk2 activity and promoting focal adhesion Cell cycle (IF 3.699) Pub Date : 2020-12-03 Meng Zhu; Bingke Lv; Wenjing Ge; Zhenwen Cui; Kai Zhao; Yugong Feng; Xuejun Yang
ABSTRACT Store-operated Ca2+ entry (SOCE) plays an important role in regulating Ca2+ influx, which participates in tumor cell survival and motility. We aim to elucidate the role of SOCE in the behavior of C6 glioma cells. Lentiviral vector inserted with the Orai1-targeting shRNA was used to inhibit SOCE in C6 glioma cells. The down-regulation of Orai1 was confirmed by western blot. The ability of shOrai1
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Crocin inhibits the activation of mouse hepatic stellate cells via the lnc-LFAR1/MTF-1/GDNF pathway Cell cycle (IF 3.699) Pub Date : 2020-12-09 Ji Xuan; Dongmei Zhu; Zhengyuan Cheng; Yuping Qiu; Mei Shao; Ya Yang; Qi Zhai; Fangyu Wang; Feng Qin
ABSTRACT Crocin is the main monomer of saffron, which is a momentous component of traditional Chinese medicine Lang Qing A Ta. Here, we tried to probe into the role of crocin in liver fibrosis. Hematoxylin-eosin staining and Sirius Red staining were used to observe the pathological changes of liver tissues. After hepatic stellate cells (HSCs) were isolated from liver tissues, lnc-LFAR1, MTF-1, GDNF
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Human CTC1 promotes TopBP1 stability and CHK1 phosphorylation in response to telomere dysfunction and global replication stress Cell cycle (IF 3.699) Pub Date : 2020-12-03 Stephanie M. Ackerson; Caroline I. Gable; Jason A. Stewart
ABSTRACT CST (CTC1-STN1-TEN1) is a heterotrimeric, RPA-like complex that binds to single-stranded DNA (ssDNA) and functions in the replication of telomeric and non-telomeric DNA. Previous studies demonstrated that deletion of CTC1 results in decreased cell proliferation and telomere DNA damage signaling. However, a detailed analysis of the consequences of conditional CTC1 knockout (KO) has not been
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P53 induces senescence in the unstable progeny of aneuploid cells Cell cycle (IF 3.699) Pub Date : 2020-12-11 Maybelline Giam; Cheng Kit Wong; Jun Siong Low; Matteo Sinelli; Oliver Dreesen; Giulia Rancati
ABSTRACT Aneuploidy is the condition of having an imbalanced karyotype, which is associated with tumor initiation, evolution, and acquisition of drug-resistant features, possibly by generating heterogeneous populations of cells with distinct genotypes and phenotypes. Multicellular eukaryotes have therefore evolved a range of extrinsic and cell-autonomous mechanisms for restraining proliferation of
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Thioridazine hydrochloride: an antipsychotic agent that inhibits tumor growth and lung metastasis in triple-negative breast cancer via inducing G0/G1 arrest and apoptosis Cell cycle (IF 3.699) Pub Date : 2020-12-14 Yanlin Song; Lu Li; Jiao Chen; Hongli Chen; Bomiao Cui; Yun Feng; Ping Zhang; Qiangsheng Zhang; Yong Xia; Min Luo
ABSTRACT Triple-negative breast cancer (TNBC) is the most aggressive type of breast cancer and still lacks available targeted therapy options. Thioridazine hydrochloride (Thi-hyd), a common phenothiazine antipsychotic drug, has shown great tumor-suppressive potential in several types of cancer cells. In this study, we explored the anti-tumor effects of Thi-hyd on TNBC. Thi-hyd significantly inhibited
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The pro-migration and anti-apoptosis effects of HMGA2 in HUVECs stimulated by hypoxia Cell cycle (IF 3.699) Pub Date : 2020-12-14 Zhu-Lan Cai; Chen Liu; Qi Yao; Qing-We Xie; Tong-Tong Hu; Qing-Qing Wu; Qi-Zhu Tang
ABSTRACT High-mobility group AT-hook2 (HMGA2), serving as an architectural transcription factor, participates in plenty of biological processes. Our study is aimed at illustrating the effect of HMGA2 on hypoxia-induced HUVEC injury and the underlying mechanism. To induce hypoxia-related cell injury, HUVECs were exposed to hypoxic condition for 12–24 h. Molecular expression was determined by Western
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Promoting roles of long non-coding RNA FAM83H-AS1 in bladder cancer growth, metastasis, and angiogenesis through the c-Myc-mediated ULK3 upregulation Cell cycle (IF 3.699) Pub Date : 2020-12-08 Beibei Liu; Wuyue Gao; Wei Sun; Liqiang Li; Chao Wang; Xiaohuai Yang; Jianmin Liu; Yuanyuan Guo
ABSTRACT Long non-coding RNA (lncRNA) FAM83H-AS1 has been recently identified with oncogenic roles in many human cancers. But its role in bladder cancer (BCa) pathogenesis and the mechanisms are largely unstudied. This study aims to evaluate the roles of FAM83H-AS1 in the malignant behaviors and the angiogenesis of BCa cells and the mechanical molecules involved. High expression of FAM83H-AS1 was found
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The circ_0004463/miR-380-3p/FOXO1 axis modulates mitochondrial respiration and bladder cancer cell apoptosis Cell cycle (IF 3.699) Pub Date : 2020-12-07 Shuiqing Wu; Huanghao Deng; Haiqing He; Ran Xu; Yinhuai Wang; Xuan Zhu; Jinhua Zhang; Qi Zeng; Xiaokun Zhao
ABSTRACT Bladder cancer is one of the most commonly diagnosed and fatal malignancies of the urinary tract. Noncoding RNAs have been reported to be new biomarkers and effective treatment targets for bladder cancer. In the present study, we identified a novel bladder cancer-related circRNA-miRNA-mRNA network, the circ_0004463/miR-380-3p/FOXO1 axis. circ_0004463 is significantly downregulated, whereas
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Gefitinib encapsulation based on nano-liposomes for enhancing the curative effect of lung cancer Cell cycle (IF 3.699) Pub Date : 2020-12-10 Yanjie Hu; Jisong Zhang; Huihui Hu; Shan Xu; Li Xu; Enguo Chen
ABSTRACT Gefitinib (GEB) is one of the drugs used for patients with epidermal growth factor receptor (EGFR)-positive mutations in non-small cell lung cancer (NSCLC). However, application of GEB is limited by its low water solubility, stability, and utilization rate, especially the side effects while GEB is given by oral. In this study, nanoliposome was used as a carrier to prepare nanoliposome compound
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Combined use of microwave ablation and cell immunotherapy induces nonspecific immunity of hepatocellular carcinoma model mice Cell cycle (IF 3.699) Pub Date : 2020-12-07 Xuhua Duan; Manzhou Wang; Xinwei Han; Jianzhuang Ren; Guohao Huang; Shuguang Ju; Qinghui Zhang
ABSTRACT Microwave ablation (MWA) has been widely used in the treatment of solid tumors. Studies have been less conducted on the efficacy of MWA used with cell immunotherapy in treating hepatocellular carcinoma (HCC). The current study aimed at exploring the efficacy of MWA in combination with cell immunotherapy in treating HCC. Hepa1-6 HCC mice were treated by MWA, blockade, or the combined therapy
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MiR-17-5p promotes the endothelialization of endothelial progenitor cells to facilitate the vascular repair of aneurysm by regulating PTEN-mediated PI3K/AKT/VEGFA pathway Cell cycle (IF 3.699) Pub Date : 2020-12-14 Ye Tian; Xinxi Li; Chao Bai; Zhenwei Yang; Lei Zhang; Jun Luo
ABSTRACT The endothelialization of endothelial progenitor cells (EPCs) was proven to facilitate the vascular repair of aneurysm. MiR-17-5p regulated angiogenesis in various cancers. This research focused on exploring the effect of miR-17-5p on EPCs and the vascular repair of aneurysm. In vivo study: the aneurysm rat model was established and treated with AgomiR-17-5p; the histopathology of aneurysm
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DCAF13 promotes triple-negative breast cancer metastasis by mediating DTX3 mRNA degradation Cell cycle (IF 3.699) Pub Date : 2020-12-10 Jiazhe Liu; Hongchang Li; Anwei Mao; Jingfeng Lu; Weiyan Liu; Jingbo Qie; Gaofeng Pan
ABSTRACT DCAF13 is firstly identified as a substrate receptor of CUL4-DDB1 E3 ligase complex. This study disclosed that DCAF13 acted as a novel RNA binding protein (RBP) that contributed to triple-negative breast cancer (TNBC) metastasis. Clinical data obtained from TCGA and our collection showed that DCAF13 was closely correlated with poor clinicopathological characteristics and overall survival,
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Clinical stage molecule PT150 is a modulator of glucocorticoid and androgen receptors with antiviral activity against SARS-CoV-2. Cell cycle (IF 3.699) Pub Date : 2020-12-11 Neil D. Theise; Anthony R. Arment; Dimple Chakravarty; John M. H. Gregg; Ira M. Jacobson; Kie Hoon Jung; Sujit S. Nair; Ashutosh K. Tewari; Archie W. Thurston; John Van Drie; Jonna B. Westover
ABSTRACT PT150 is a clinical-stage molecule, taken orally, with a strong safety profile having completed Phase 1 and Phase 2 clinical trials for its original use as an antidepressant. It has an active IND for COVID-19. Antiviral activities have been found for PT150 and other members of its class in a variety of virus families; thus, it was now tested against SARS-CoV-2 in human bronchial epithelial
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Knockdown of miR-1275 protects against cardiomyocytes injury through promoting neuromedin U type 1 receptor Cell cycle (IF 3.699) Pub Date : 2020-12-15 Zhu Zeng; Haixin Ma; Jing Chen; Nina Huang; Yudan Zhang; Yufei Su; Huifang Zhang
ABSTRACT The present study aimed to assess the role of miR-1275 in cardiac ischemia reperfusion injury. H9 human embryonic stem cell (hESC)-derived cardiomyocytes stimulated by oxygen-glucose deprivation/reoxygenation (OGD/R) were used to simulate myocardial injury in vitro. miR-1275 expression levels in cells were measured by RT-qPCR. The release of lactate dehydrogenase (LDH) and creatine kinase
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Correction Cell cycle (IF 3.699) Pub Date : 2020-11-29
(2020). Correction. Cell Cycle: Vol. 19, No. 24, pp. 3650-3652.
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MicroRNAs and SARS-CoV-2 life cycle, pathogenesis, and mutations: biomarkers or therapeutic agents? Cell cycle (IF 3.699) Pub Date : 2020-12-31 Farshad Abedi; Ramin Rezaee; A. Wallace Hayes; Somayyeh Nasiripour; Gholamreza Karimi
ABSTRACT To date, proposed therapies and antiviral drugs have been failed to cure coronavirus disease 2019 (COVID-19) patients. However, at least two drug companies have applied for emergency use authorization with the United States Food and Drug Administration for their coronavirus vaccine candidates and several other vaccines are in various stages of development to determine safety and efficacy.
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Spironolactone-induced XPB degradation requires TFIIH integrity and ubiquitin-selective segregase VCP/p97 Cell cycle (IF 3.699) Pub Date : 2020-12-31 Anil K. Chauhan; Ping Li; Yingming Sun; Gulzar Wani; Qianzheng Zhu; Altaf A. Wani
ABSTRACT Mineralocorticoid and androgen receptor antagonist, spironolactone, was recently identified as an inhibitor of nucleotide excision repair (NER), acting via induction of proteolysis of TFIIH component Xeroderma Pigmentosum B protein (XPB). This activity provides a strong rationale for repurposing spironolactone for cancer therapy. Here, we report that the spironolactone-induced XPB proteolysis
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Sustained mTORC1 activity during palbociclib-induced growth arrest triggers senescence in ER+ breast cancer cells Cell cycle (IF 3.699) Pub Date : 2020-12-28 Reeja S. Maskey; Fang Wang; Elyssa Lehman; Yiqun Wang; Natasha Emmanuel; Wenyan Zhong; Guixian Jin; Robert T. Abraham; Kim T. Arndt; Jeremy S. Myers; Anthony Mazurek
ABSTRACT Palbociclib, a selective CDK4/6 kinase inhibitor, is approved in combination with endocrine therapies for the treatment of advanced estrogen receptor positive (ER+) breast cancer. In pre-clinical cancer models, CDK4/6 inhibitors act primarily as cytostatic agents. In two commonly studied ER+ breast cancer cell lines (MCF7 and T47D), CDK4/6 inhibition drives G1-phase arrest and the acquisition
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Pharmacological inactivation of CDK2 inhibits MYC/BCL-XL-driven leukemia in vivo through induction of cellular senescence Cell cycle (IF 3.699) Pub Date : 2020-12-27 Wesam Bazzar; Matteo Bocci; Eduar Hejll; Vedrana Högqvist Tabor; Per Hydbring; Alf Grandien; Mohammad Alzrigat; Lars-Gunnar Larsson
ABSTRACT Deregulated expression of the MYC oncogene is a frequent event during tumorigenesis and generally correlates with aggressive disease and poor prognosis. While MYC is a potent inducer of apoptosis, it often suppresses cellular senescence, which together with apoptosis is an important barrier against tumor development. For this latter function, MYC is dependent on cyclin-dependent kinase 2 (CDK2)
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Inhibited microRNA-494-5p promotes proliferation and suppresses senescence of nucleus pulposus cells in mice with intervertebral disc degeneration by elevating TIMP3 Cell cycle (IF 3.699) Pub Date : 2020-12-22 Gang Chen; Xiaopeng Zhou; Hao Li; Zhengkuan Xu
ABSTRACT It has been unraveled that microRNAs (miRNAs) played crucial roles in processes of human diseases, while the role of miR-494-5p in intervertebral disc degeneration (IDD) remains scarcely studied. We aimed to investigate the mechanisms of miR-494-5p in IDD with the involvement of tissue inhibitor of metalloproteinase 3 (TIMP3). Expression of miR-494-5p and TIMP3 in IDD clinical specimens was
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IncRNA ZFAS1 contributes to the radioresistance of nasopharyngeal carcinoma cells by sponging hsa-miR-7-5p to upregulate ENO2 Cell cycle (IF 3.699) Pub Date : 2020-12-20 Jiaojiao Peng; Feng Liu; Hong Zheng; Qi Wu; Shixi Liu
ABSTRACT Previous research revealed that lncRNA ZFAS1 could promote nasopharyngeal carcinoma (NPC) by inhibiting its downstream target axis. However, the association between ZFAS1 and radioresistant NPC cells is unclear. This study aimed to explore the roles of ZFAS1 in the radioresistance of NPC. Bioinformatics analysis was conducted to identify the significant factors (ENO2 and miR-7-5p) that contributed
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Down-regulating microRNA-20a regulates CDH1 to protect against cerebral ischemia/reperfusion injury in rats Cell cycle (IF 3.699) Pub Date : 2020-12-19 Chun-chun Yang; Xiang-pin Wei; Xian-ming Fu; Ling-tao Qian; Lan-jun Xie; Hong-bo Liu; Gang Li; Xin-gang Li; Xian-wei Zeng
ABSTRACT Studies have extensively focused on the involvement of microRNAs (miRNAs) in cerebral ischemia/reperfusion (I/R) injury but not much on the specific role of miR-20a. Hence, this study is purposed to decipher whether miR-20a could regulate cadherin 1 (CDH1) to affect cerebral I/R injury in rats. Rat transient middle cerebral artery occlusion model (MCAO) was established. Rats were injected
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Long non-coding RNA XIST promotes the progression of esophageal squamous cell carcinoma through sponging miR-129-5p and upregulating CCND1 expression Cell cycle (IF 3.699) Pub Date : 2020-12-19 Haoran Wang; Haomiao Li; Yongkui Yu; Qingfeng Jiang; Ruixiang Zhang; Haibo Sun; Wenqun Xing; Yin Li
ABSTRACT Long non-coding RNA (lncRNA) X inactive specific transcript (XIST) has been identified as an oncogenic lncRNA in a series of human cancers, including esophageal squamous cell carcinoma (ESCC). In this study, we aimed to further explore the underlying mechanism of XIST on ESCC progression. qRT-PCR assay was used to determine the levels of XIST and miR-129-5p. Western blot analysis was performed
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The possible role of Sirtuins and microRNAs in hepatocellular carcinoma therapy Cell cycle (IF 3.699) Pub Date : 2020-11-09 Hedyieh Karbasforooshan; A. Wallace Hayes; Nooshin Mohammadzadeh; Mohammad Reza Zirak; Gholamreza Karimi
ABSTRACT Hepatocellular carcinoma (HCC) is the most common primary liver cancer. Sirtuins are NAD+-dependent histone deacetylases that regulate many cellular processes such as proliferation, apoptosis, and metabolism. SIRT (silent information regulator)-1, 5, 6 and 7, members of the mammalian Sirtuin family of proteins (SIRT1–SIRT7), are involved in carcinogenesis, prognosis, metastasis, and chemical
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Regulation of hepatocyte cell cycle re-entry by RNA polymerase II-associated Gdown1 Cell cycle (IF 3.699) Pub Date : 2020-11-25 Miki Jishage; Robert G. Roeder
ABSTRACT Liver is the central organ responsible for whole-body metabolism, and its constituent hepatocytes are the major players that carry out liver functions. Although they are highly differentiated and rarely divide, hepatocytes re-enter the cell cycle following hepatic loss due to liver damage or injury. However, the exact molecular mechanisms underlying cell cycle re-entry remain undefined. Gdown1
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Overexpression of microRNA-221 promotes the differentiation of stem cells from human exfoliated deciduous teeth to neurons through activation of Wnt/β-catenin pathway via inhibition of CHD8 Cell cycle (IF 3.699) Pub Date : 2020-11-17 Bing Wen; Chenjiang He; Qin Zhang; Fanglin Zhang; Na Li; Yan Pan; Mengting Deng; Yue Wang; Jianping Li; Jiaxuan Qiu
ABSTRACT microRNAs have been proved to function in some processes of differentiation and the effect is favorable. At present, the differentiation of stem cells is not so ideal because of the high expenses and inaccessibility. Therefore, we explored the possibility that microRNA-221 (miR-221) affects differentiation from stem cells from human deciduous tooth (SHEDs) to neurons through Wnt/β-catenin
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Matrine Inhibitory Effect on Self-renewal and Re-sensitization of 5-FU Resistant NSCLC Stem Cells were through Let-7b dependent Downregulation of CCND1 Cell cycle (IF 3.699) Pub Date : 2020-11-09 Xiang Li; Meng Wang; Ning Du; Ting Liang; Guo-Dong Xiao; Kai Li; Ji-Chang Wang; Chong-Wen Xu; Zi-Yang Peng; Shou-Ching Tang; Xin Sun
ABSTRACT Matrine is one of the major alkaloids extracted from Sophora flavescens Ait of the traditional Chinese medicine, was the main chemical ingredient of compounds of Kushen injection. The Matrine is considered as a promising therapeutic agent for curing nonsmall cell lung cancer (NSCLC), used either alone or combined with chemotherapeutic agents. In the present study, we focused on the possible
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microRNA-877 contributes to decreased non-small cell lung cancer cell growth via the PI3K/AKT pathway by targeting tartrate resistant acid phosphatase 5 activity Cell cycle (IF 3.699) Pub Date : 2020-11-23 Xue Bai; Changjun He; Bicheng Fu; Xianglong Kong; Jianlong Bu; Kaibin Zhu; Wei Zheng; Fucheng Zhou; Boxiong Ni
ABSTRACT Non-small cell lung cancer (NSCLC) is a leading cause of cancer death in both men and women. microRNAs (miRs) can exert important functions in cancer development. However, the role of miR-877 in NSCLC as it relates to tartrate resistant acid phosphatase 5 (ACP5) is unknown. For this study, the gain-and-loss-of-function experiments were performed to explore the effects of miR-877 and ACP5 on
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Long noncoding RNA MEG3 suppresses cell proliferation, migration and invasion, induces apoptosis and paclitaxel-resistance via miR-4513/PBLD axis in breast cancer cells Cell cycle (IF 3.699) Pub Date : 2020-10-30 Mingzhi Zhu; Fang Wang; Hailong Mi; Lin Li; Jing Wang; Mingli Han; Yuanting Gu
ABSTRACT Breast cancer remains a general-threat event in the health of women. Currently, increasing records indicate that long non-coding RNA maternally expressed 3 (MEG3) plays a central role in breast cancer. The current research focused on the function of MEG3 in paclitaxel (PTX)-resistance and human breast cancer growth. Levels of MEG3, microRNA (miR)-4513, and phenazine biosynthesis-like domain-containing
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Depletion of circ_0007841 inhibits multiple myeloma development and BTZ resistance via miR-129-5p/JAG1 axis Cell cycle (IF 3.699) Pub Date : 2020-11-01 Yan Wang; Quande Lin; Chunge Song; Ruojin Ma; Xiaojie Li
ABSTRACT Circular RNAs (circRNAs) possess important regulatory effects on multiple myeloma (MM) progression. Here, we aimed at exploring the function of circ_0007841 in MM and the underlying molecular mechanism. Expression of circ_0007841, microRNA (miR)-129-5p and Jagged1 (JAG1) was determined via qRT-PCR or western blot assay. Methyl thiazolyl tetrazolium (MTT) assay was applied to examine cell viability
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Circ_0008450 downregulates Runx3 to promote the proliferation and epithelial-mesenchymal transition of human keratinized epithelial cells Cell cycle (IF 3.699) Pub Date : 2020-11-01 Huaxia Chen; Xiao Xu; Linying Lai; Ran Huo; Minliang Chen
ABSTRACT Keloid is an extremely common and often overlooked benign neoplastic disease, but its consequences should not be underestimated. Therefore, a deep exploration of the pathological mechanism of keloid becomes very essential. After 22 samples were collected from each patient’s keloid tissues and normal skin tissues, circ_0008450 and Runx3 expression was tested by qRT-PCR. When primary human keratinized
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The long non-coding RNA TSLC8 inhibits colorectal cancer by stabilizing puma Cell cycle (IF 3.699) Pub Date : 2020-11-20 Zhian Du; Tao Yu; Meina Sun; Yun Chu; Gang Liu
ABSTRACT The colorectal cancer (CRC) dictates a common malignancy with high recurrence rate. Long non-coding RNAs (lncRNAs) belong to a class of regulatory factors involved in multiple cancers. In current work, we have uncovered a novel lncRNA named TSLC8. TSLC8 was dramatically downregulated in CRC samples and cell lines. Reintroduction of TSLC8 inhibited tumor sphere formation and viability in CRC
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Baicalin reversal of DNA hypermethylation-associated Klotho suppression ameliorates renal injury in type 1 diabetic mouse model Cell cycle (IF 3.699) Pub Date : 2020-11-16 Xiao-Tan Zhang; Guang Wang; Liu-Fang Ye; Yu Pu; Run-Tong Li; Jianxin Liang; Lijun Wang; Kenneth Ka Ho Lee; Xuesong Yang
ABSTRACT Baicalin is a flavone glycoside that possesses numerous pharmacological properties. but its protective mode of action in kidney injury induced by diabetes mellitus remains incompletely understood. Using a streptozotocin (STZ)-induced diabetic mouse model, we found that baicalin could ameliorate diabetes-induced the pathological changes of the kidney function and morphology through suppressing
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SEC61G plays an oncogenic role in hepatocellular carcinoma cells Cell cycle (IF 3.699) Pub Date : 2020-11-10 Huijie Gao; Weibo Niu; Zhaobin He; Chao Gao; Cheng Peng; Jun Niu
ABSTRACT Hepatocellular carcinoma (HCC) is one of the most aggressive malignant diseases and requires more effective prevention and treatment strategies. Mutations or overexpression of endoplasmic reticulum (ER) proteins have been frequently identified in a solid tumor, suggesting that ER proteins play an important role in tumor development. SEC61G, a component of Sec61 complex located in the membrane
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Identification of curcumin as a novel natural inhibitor of rDNA transcription Cell cycle (IF 3.699) Pub Date : 2020-11-10 Yinfeng Xu; Yaosen Wu; Lei Wang; Chuying Qian; Qian Wang; Wei Wan
ABSTRACT Ribosomal DNA (rDNA) transcription drives cell growth and cell proliferation via the product ribosomal RNA (rRNA), the essential component of ribosome. Given the fundamental role of rRNA in ribosome biogenesis, rDNA transcription has emerged as one of the effective targets for a number of human diseases including various types of cancers. In this study, we identify curcumin, an ancient drug
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Mechanism of turnover or persistence of radiation-induced myofibroblast in vitro Cell cycle (IF 3.699) Pub Date : 2020-11-22 Tsutomu Shimura; Takahito Ando; Momoka Narao; Megumi Sasatani; Kenji Kamiya; Akira Ushiyama
ABSTRACT We recently made an important discovery that radiation induces myofibroblasts, which play a role in radiation-related carcinogenesis via tumor microenvironment formation. Here, we investigated the threshold dose and the mechanisms of myofibroblast induction to assess adverse radiation effects on normal cells. Single-dose of healthy human fibroblasts in vitro promotes myofibroblast induction
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Effect of cell cycle synchronization on cadmium-induced apoptosis and necrosis in NRK-52E cells Cell cycle (IF 3.699) Pub Date : 2020-11-23 Tongwang Luo; Qi Yu; Wenxuan Dong; Zhonggui Gong; Yun Tan; Wenjing Liu; Hui Zou; Jianhong Gu; Yan Yuan; Jianchun Bian; Chunyan Shao; Jiaqiao Zhu; Zongping Liu
ABSTRACT Heavy metal pollution is a problem that cannot be ignored. Due to the prevalence of cadmium in the environment and its harmful effects on humans, cadmium pollution has become a research hotspot recently. The mechanism of cadmium-induced toxicity has also drawn much attention and most studies have been conducted using whole cells, but the toxicological mechanism of cadmium remains unclear.
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