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A Plastic β-Tricalcium Phosphate/Gelatine Scaffold Seeded with Allogeneic Adipose-Derived Stem Cells for Mending Rabbit Bone Defects Cell. Reprogram. (IF 1.702) Pub Date : 2021-01-05 Jia Liu; Peng Zhou; Jane Smith; Saiqun Xu; Chunxia Huang
To investigate the feasibility of β-tricalcium phosphate (TCP)/gelatine scaffold combined with allogeneic adipose-derived stem cells (ASCs) to repair hole shape defect, third-passage ASCs were seeded onto composite scaffolds to prepare an ASC-β-TCP/gelatine tissue-engineered bone to pack into the rabbit cavernous bone defects of experimental groups. In animal models, the bone defect area was completely
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Effects of Edaravone on Functional Recovery of a Rat Model with Spinal Cord Injury Through Induced Differentiation of Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells Cell. Reprogram. (IF 1.702) Pub Date : 2021-01-05 Yumei Li; Laibing Liu; Zijiang Yu; Yan Yu; Baofei Sun; Chaolun Xiao; Shipeng Luo; Lin Li
Edaravone can induce differentiation of bone marrow mesenchymal stem cells (BMSCs) into neuron-like cells and replace lost cells by transplanting neuron-like cells to repair spinal cord injury (SCI). In this study, BMSCs were derived from the bone marrow of male Wistar rats (4 weeks old) through density gradient centrifugation (1.073 g/mL), and the cell purity of BMSCs was up to 95%. The combined injection
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Generation of Keratinocytes from Human Induced Pluripotent Stem Cells Under Defined Culture Conditions Cell. Reprogram. (IF 1.702) Pub Date : 2020-12-29 Shyam Kishor Sah; Jitendra K. Kanaujiya; I-Ping Chen; Ernst J. Reichenberger
Differentiation of keratinocytes from human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) has become an important tool for wound healing research and for studying skin diseases in instances where patient cells are not available. Several keratinocyte differentiation protocols using hiPSC colony fragments or embryoid bodies have been published with some requiring prolonged
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Low-Concentration Essential Amino Acids in PZM-3 Improve the Developmental Competence of Porcine Embryos Produced by Handmade Cloning Cell. Reprogram. (IF 1.702) Pub Date : 2020-12-02 Tianbin Liu; Jie Li; Hongwei Dou; Xi Xiang; Wenbin Chen; Tingting Zhang; Lin Li; Xingju Zhang; Xuan Dong; Lei Chen; Xuyi Lin; Jing Li; Hai-Xi Sun; Ying Gu; Lin Lin
Essential amino acids (EAA) of inappropriate concentration have been reported to compromise the development of embryo. This study aimed to investigate the effect of EAA on the developmental competence of porcine embryos produced by either handmade cloning (HMC) or parthenogenetic activation (PA). In experiment 1, we examined the in vitro developmental competence of PA embryos after culture in PZM-3
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Effect of Rhodiola sachalinensis Aqueous Extract on In Vitro Maturation of Porcine Oocytes and Subsequent In Vitro Embryonic Development Cell. Reprogram. (IF 1.702) Pub Date : 2020-12-02 Caifeng Wu; Defu Zhang; Shushan Zhang; Lingwei Sun; Ying Liu; Jianjun Dai
Oxidative stress can impede maturation of the nucleus and cytoplasm of oocytes during in vitro maturation (IVM). Rhodiola sachalinensis, an herb commonly used in traditional Chinese medicine, conveys antioxidative effects to cryopreserved bovine sperm. Therefore, the aims of this study were to evaluate the effects of different concentrations of R. sachalinensis aqueous extract (RSAE) on IVM and subsequent
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Metabolic Enhancement of Glycolysis and Mitochondrial Respiration Are Essential for Neuronal Differentiation Cell. Reprogram. (IF 1.702) Pub Date : 2020-12-02 Ding Li; Zhexu Ding; Manjin Gui; Yanmei Hou; Kui Xie
Metabolic reactions provide energy and metabolic substance for cell function. It was recently shown that metabolic reprogramming is a key regulator of cell pluripotency and differentiation. Although many evidences point to a metabolic “switch” toward mitochondrial respiration, the importance of glycolysis and mitochondrial respiration is still controversial. In this study, we differentiated two different
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Going Back and Forth: Episomal Vector Reprogramming of Peripheral Blood Mononuclear Cells to Induced Pluripotent Stem Cells and Subsequent Differentiation into Cardiomyocytes and Neuron-Astrocyte Co-cultures Cell. Reprogram. (IF 1.702) Pub Date : 2020-12-02 Victoria C. de Leeuw; Conny T.M. van Oostrom; Sandra Imholz; Aldert H. Piersma; Ellen V.S. Hessel; Martijn E.T. Dollé
Human induced pluripotent stem cells (iPSCs) can capture the diversity in the general human population as well as provide deeper insight in cellular mechanisms. This makes them suitable to study both fundamental and applied research subjects, such as disease modeling, gene-environment interactions, personalized medicine, and chemical toxicity. In an independent laboratory, we were able to generate
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Distinctive Cellular Transcriptomic Signature and MicroRNA Cargo of Extracellular Vesicles of Horse Adipose and Endometrial Mesenchymal Stem Cells from the Same Donors Cell. Reprogram. (IF 1.702) Pub Date : 2020-12-02 Felipe Navarrete; Yat Sen Wong; Joel Cabezas; Gonzalo Riadi; José Manríquez; Daniela Rojas; Ana Carolina Furlanetto Mançanares; Lleretny Rodriguez-Alvarez; Fernando Saravia; Fidel Ovidio Castro
Equine endometrial and adipose mesenchymal stem cells (eMSCs and aMSCs, respectively) were isolated from the same donors of thoroughbred mares. The cells displayed characteristic features of MSCs, including trilineage mesodermal and also neurogenic differentiation. We evaluated the influence of cellular origin on their transcriptome profile. Cellular RNA was isolated and sequenced and extracellular
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MiR-142-5p Suppresses Lung Cancer Cell Metastasis by Targeting Yin Yang 1 to Regulate Epithelial–Mesenchymal Transition Cell. Reprogram. (IF 1.702) Pub Date : 2020-12-02 Qiao Zhang; Hongfei Liu; Jian Zhang; Li Shan; Bumaireyimu Yibureyimu; Alima Nurlan; Patiguli Aerxiding; Qin Luo
This study aimed to investigate the mechanism of miR-142-5p and Yin Yang 1 (YY1) on regulating epithelial–mesenchymal transition (EMT) in lung cancer cell metastasis. The expressions of YY1 and miR-142-5p in different lung cancer cell lines were negatively correlated. The results of the dual-luciferase reporter assay further validated that miR-142-5p directly targeted YY1. Subsequently, transwell assays
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Optimization and Comparison of Three-Dimensional Culture Conditions in Different Media of Coculture and Encapsulation System for In Vitro Follicular Development in Bubalus bubalis Cell. Reprogram. (IF 1.702) Pub Date : 2020-11-04 Diksha Dua; Gaurav Tripathi; Afroz Alam; Manmohan Singh Chauhan; Prabhat Palta; Manoj Kumar Singh
The establishment of an in vitro culture system for complete oocyte maturation from the early stages of ovarian follicles is still a challenge. The aim of the present study was to assess the effect of different matrix with different culture media on the developmental growth of ovarian follicles in vitro. An ovarian histoarchitectural study was carried out to identify the primordial (0.027–0.039 mm)
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A Comparative Approach of Cellular Reprogramming in the Rodentia Order. Cell. Reprogram. (IF 1.702) Pub Date : 2020-09-30 Érika Almeida Praxedes,Fabiana Fernandes Bressan,Alexsandra Fernandes Pereira
Cellular reprogramming mainly involves induction of reactivation of genes responsible for nuclear plasticity, a process that can be performed in vitro through production of cloned embryos by somatic cell nuclear transfer or by induction of cells into the pluripotent state through exogenous transcription factor expression. While these techniques are already well known and utilized in mice and rats,
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Effect of Additional Cytoplasm of Cloned Embryo on In Vitro Developmental Competence and Reprogramming Efficiency in Mice. Cell. Reprogram. (IF 1.702) Pub Date : 2020-09-30 Seok-Hwan Song,Seon-Hwa Oh,Lianguang Xu,Kyeong-Lim Lee,Ji-Yoon Hwang,Myeong-Don Joo,Il-Keun Kong
Somatic cell nuclear transfer (SCNT) is an important technique for biological science research. Cytoplasm injection cloning technology (CICT) was developed to improve the reprogramming efficiency as well as to overcome the limitations of SCNT. CICT uses an additional cytoplasm fused with an enucleated oocyte to restore the cytoplasmic volume of the cloned embryo, and this method could improve the reprogramming
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Effects of Dimethyl Sulfoxide on the Pluripotency and Differentiation Capacity of Mouse Embryonic Stem Cells. Cell. Reprogram. (IF 1.702) Pub Date : 2020-09-30 Jun-Koo Yi,Song Park,Jae-Jung Ha,Dae-Hyun Kim,Hai Huang,Si-Jun Park,Mee-Hyun Lee,Zae-Young Ryoo,Sung-Hyun Kim,Myoung-Ok Kim
Mouse embryonic stem cells (mESCs) go through self-renewal in the existence of the cytokine leukemia inhibitory factor (LIF). LIF is added to the mouse stem cells culture medium, and its removal results in fast differentiation. Dimethyl sulfoxide (DMSO) is one of the most used solvents in drug test. We exposed 4-day mESC cultures to different concentrations of DMSO (0.1%, 0.5%, 1.0%, and 2.0%) to identify
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The Extracts of Human Fetal Brain Induce the Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Dopaminergic Neuron Containing Cells. Cell. Reprogram. (IF 1.702) Pub Date : 2020-09-30 Yonghai Li,Junzheng Yang,Meng Li,Xiaoyue Zhang,Jiang Du,Xinghua Zhao,Zhihao Xu,Juntang Lin
Mesenchymal stem cells (MSCs) have the potential to differentiate into neuron-like cells, which may provide a new strategy for the clinical treatment of neurodegenerative diseases such as Parkinson's disease (PD). However, the application of MSCs in the patients is still limited as the reason of efficiency and safety of transplantation. The aim of this study is to develop a new method and induce human
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Reprogramming Prostate Cancer Cells into Induced Pluripotent Stem Cells: a Promising Model of Prostate Cancer Stem Cell Research. Cell. Reprogram. (IF 1.702) Pub Date : 2020-09-30 Yiming Zhang,Binshen Chen,Peng Xu,Chunxiao Liu,Peng Huang
Prostate cancer stem cells (PrCSCs) are responsible for the development of castration-resistant disease and are associated with poor outcomes; however, the origin of PrCSCs is still not known due to the lack of a suitable model. In the current study, the human prostate cancer cell line 22RV1 was used to generate induced pluripotent stem cells (iPSCs) via the exogenous expression of four classic transcription
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Bone Marrow Mesenchymal Stem Cells Promote the Stemness of Hypopharyngeal Cancer Cells. Cell. Reprogram. (IF 1.702) Pub Date : 2020-09-30 Danyang Li,Yiming Liu,Jinyan Qi,Xinhua Cui,Ying Guo,Dipanpan Wu,Hui Liang
A study was to investigate the regulation of bone marrow mesenchymal stem cells (BMSCs) on the stemness of hypopharyngeal cancer cells (FaDu cells). Green fluorescent protein-labeled FaDu cells were cocultured with BMSCs and then were isolated. In vitro experiments, including cell cycle and apoptosis analyses and clonogenic and sphere formation assays, were conducted using the cocultured FaDu cells
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Generation of Induced Pluripotent Stem Cells from a Female Patient with a Xq27.3-q28 Deletion to Establish Disease Models and Identify Therapies. Cell. Reprogram. (IF 1.702) Pub Date : 2020-08-05 Noriko Watanabe,Kohei Kitada,Katherine E Santostefano,Airi Yokoyama,Sara M Waldrop,Coy D Heldermon,Daisuke Tachibana,Masayasu Koyama,Amy M Meacham,Christina A Pacak,Naohiro Terada
Since it is extremely difficult to establish an animal model for human chromosomal abnormalities, induced pluripotent stem cells (iPSCs) provide a powerful alternative to study underlying mechanisms of these disorders and identify potential therapeutic interventions. In this study we established iPSCs from a young girl with a hemizygous deletion of Xq27.3-q28 who exhibited global developmental delay
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Effect of Aerobic and Resistance Training on Endothelial Progenitor Cells in Mice with Type 2 Diabetes. Cell. Reprogram. (IF 1.702) Pub Date : 2020-08-05 Xia Dai,Lu Zhai,Qiang Su,BeiBei Luo,Chun Wei,Yuhua Liu,Yanfeng Huang,Cui Ma,Yanping Ying
Since no study has explored whether exercise could improve impaired proliferation, migration, and angiogenesis of endothelial progenitor cells (EPCs) in animal models or humans with type 2 diabetes, we aimed to explore the effect of different models of exercise on EPC function and expression of caveolin-1, PI3K, and AKT in mice with type 2 diabetes. Male db/db mice (age: 8 weeks) with type 2 diabetes
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The Effect of L-Carnitine Additive During In Vitro Maturation on the Vitrification of Pig Oocytes. Cell. Reprogram. (IF 1.702) Pub Date : 2020-08-05 Hongxia Xu,Chao Jia,Wenxiu Cheng,Tongtong Zhang,Ruixin Tao,Yuehua Ma,Linan Si,Yinxue Xu,Juan Li
Cryopreservation of oocytes/embryos is an important technique for genetic resources; however, the success of vitrification in pig oocytes remained at a relatively lower level due to the high content of lipid droplets (LDs). Considering the positive effect of L-carnitine on the function of LDs, the present study was designed to investigate the effect of the addition of L-carnitine on the vitrification
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In Vitro Development and Mitochondrial Gene Expression in Brown Brocket Deer (Mazama gouazoubira) Embryos Obtained by Interspecific Somatic Cell Nuclear Transfer. Cell. Reprogram. (IF 1.702) Pub Date : 2020-08-05 Lívia C Magalhães,Jenin V Cortez,Maajid H Bhat,Ana Clara N P C Sampaio,Jeferson L S Freitas,José M B Duarte,Luciana M Melo,Vicente J F Freitas
The genetic diversity of Neotropical deer is increasingly jeopardized, owing to declining population size. Thus, the formation of cryobanking of somatic cells is important for the preservation of these species using cloning. The transformation of these cells into viable embryos has been hampered by a lack of endangered species oocytes. Accordingly, the aim of this study was to produce brown brocket
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Activation of Wnt/β-Catenin Signaling Pathway Enhances the Derivation of Buffalo (Bubalus bubalis) Embryonic Stem Cell-Like Cells. Cell. Reprogram. (IF 1.702) Pub Date : 2020-08-05 Yanfei Deng,Yanping Lao,Qiuyan Ruan,Jun Zhang,Chan Luo,Deshun Shi,Fenghua Lu
Wnt/β-Catenin signaling pathway plays an important role in maintaining self-renewal and pluripotency of human and mouse embryonic stem cells (ESCs). Activation of Wnt/β-Catenin signaling pathway by glycogen synthase kinase-3 (GSK3) inhibitor, the Wnt signaling agonist, could maintain the pluripotency of human and mouse ESCs in the presence of serum. However, the role of signaling pathway in the derivation
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Induction of Hepatic Regeneration in an Experimental Model Using Hepatocyte-Differentiated Mesenchymal Stem Cells. Cell. Reprogram. (IF 1.702) Pub Date : 2020-06-03 Hanan El Baz,Zeinab Demerdash,Manal Kamel,Olfat Hammam,Dina Samir Abdelhady,Soheir Mahmoud,Salwa Hassan,Faten Mahmoud,Shimaa Atta,Nermine Magdi Riad,Taghrid Gaafar
Mesenchymal stem cell (MSC)-based liver tissue engineering on nanofibrous scaffold holds great promise for cell-based therapy in liver injuries and end-stage liver failure treatments. MSCs were generated from umbilical cord blood. Hepatogenic differentiation was induced on two-dimensional (2D) and three-dimensional (3D) culture system and characterized by morphology, scanning electron microscopy, immunocytochemistry
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Differentiation of Urine-Derived Induced Pluripotent Stem Cells to Neurons, Astrocytes, and Microvascular Endothelial Cells from a Diabetic Patient. Cell. Reprogram. (IF 1.702) Pub Date : 2020-06-03 Wan Liu,Ping Zhang,Jing Tan,Yongzhong Lin
Complications of central nervous system in type 2 diabetes mellitus (T2DM) often lead to cognitive impairment and seriously affect the quality of life. However, there is no individualized disease model. Urine-derived stem cells can be an ideal source for generating human induced pluripotent stem cells (hiPSCs) and progenitors, as they are easily accessible, noninvasive, and universally available. In
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Melatonin Enhances the Development of Porcine Cloned Embryos by Improving DNA Methylation Reprogramming. Cell. Reprogram. (IF 1.702) Pub Date : 2020-06-03 Jiadan Qu,Mingjun Sun,Xiangyu Wang,Xuexiong Song,Hongbin He,Yanjun Huan
Incomplete DNA methylation reprogramming in cloned embryos leads to poor cloning efficiency. Melatonin has been proven to improve the development of cloned embryos, however, the role of melatonin during somatic cell nuclear transfer remains unclear. This work demonstrated that 10−7 M melatonin significantly enhanced the developmental progress, reduced the arrested rate before zygotic genome activation
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Hypoxia Enhances Mesenchymal Characteristics Maintenance of Buffalo Bone Marrow-Derived Mesenchymal Stem Cells. Cell. Reprogram. (IF 1.702) Pub Date : 2020-06-03 Jun Zhang,Chuan Lei,Yanfei Deng,Jam Zaheer Ahmed,Deshun Shi,Fenghua Lu
Bone marrow-derived mesenchymal stem cells (BMSCs) from livestock are valuable resources for veterinary therapeutics and animal reproduction. Previous studies have shown that hypoxic conditions were beneficial in maintaining the mesenchymal feature of BMSCs. However, the effects of hypoxia on buffalo BMSCs (bBMSCs) remain unclear. In this study, the effects of hypoxic conditions on cell morphology
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In Vitro and In Vivo Interspecies Chimera Assay Using Early Pig Embryos. Cell. Reprogram. (IF 1.702) Pub Date : 2020-06-03 Monika Nowak-Imialek,Stephanie Wunderlich,Doris Herrmann,Svenja Breitschuh-Leibling,Gudrun Gohring,Björn Petersen,Sabine Klein,Ulrich Baulain,Andrea Lucas-Hahn,Ulrich Martin,Heiner Niemann
Chimeric pigs harboring organs derived from human stem cells are promising for patient-specific regenerative therapies. Induced pluripotent stem cells (iPSCs) can contribute to all cell types of the fetus, including germline after injection into embryos. However, ethical concerns prohibit testing human iPSCs in chimera assays. Here, we evaluated porcine embryos as hosts for an interspecies chimera
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Roles of Exosomes from Mesenchymal Stem Cells in Treating Osteoarthritis. Cell. Reprogram. (IF 1.702) Pub Date : 2020-06-03 Jian Wang,Xuanxuan Guo,Zhanrong Kang,Lingbin Qi,Ying Yang,Juan Wang,Jun Xu,Shane Gao
Exosomes are small extracellular vesicles (EVs) with a diameter of 50–150 nm that play important roles in cell-to-cell communication through transportation of proteins, microRNAs, lncRNAs, and mRNAs. Some components, such as miRNAs, have been proven to be involved in inflammation regulation. Osteoarthritis (OA) is a progressive disease resulting in articular cartilage degeneration and subchondral bone
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Advances in Isolation and Culture of Chicken Embryonic Stem Cells In Vitro. Cell. Reprogram. (IF 1.702) Pub Date : 2020-03-30 Chunxia Xiong,Mingyu Wang,Wenhui Ling,Dengfeng Xie,Xinyue Chu,Yunxin Li,Yun Huang,Tong Li,Edward Otieno,Xiaoyan Qiu,Xiong Xiao
Chicken embryonic stem cells (cESCs) isolated from the egg at the stage X hold great promise for cell therapy, tissue engineering, pharmaceutical, and biotechnological applications. They are considered to be pluripotent cells with the capacity to self-renewal and differentiate into specialized cells. However, long-term maintenance of cESCs cannot be realized now, which impedes the establishment of
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Effect of Downregulating the Hippo Pathway Members YAP1 and LATS2 Transcripts on Early Development and Gene Expression Involved in Differentiation in Porcine Embryos. Cell. Reprogram. (IF 1.702) Pub Date : 2020-03-30 Natsuko Emura,Yuriko Saito,Ruri Miura,Ken Sawai
In mouse development, differentiation of the inner cell mass (ICM) and trophectoderm (TE) during the transition from the morula to blastocyst stage is regulated by the Hippo pathway; however, the functions of the Hippo pathway in porcine embryogenesis have not been investigated. In the present study, we examined the gene expression patterns of the Hippo pathway members yes-associated protein 1 (YAP1)
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The Effects of Daxx Knockout on Pluripotency and Differentiation of Mouse Induced Pluripotent Stem Cells. Cell. Reprogram. (IF 1.702) Pub Date : 2020-03-30 Hui Liu,Zhaojun Liu,Meng Gao,Xinglin Hu,Ruizhen Sun,Xinghui Shen,Feng Liu,Jingling Shen,Zhiyan Shan,Lei Lei
Induced pluripotent stem cell (iPSC) technology refers to the reprogramming of terminally differentiated somatic cells into pluripotent stem cells by introducing specific transcription factors that are known to regulate pluripotency, including Oct4, Sox2, Klf4, and c-Myc. In this study, we reprogrammed the primary fibroblasts isolated from the Daxxflox/flox mice, which carry the Oct4-green fluorescent
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Intermediate Standstill Clones Trapped in the Reprogramming of Human Fibroblasts to Induced Pluripotent Stem Cells. Cell. Reprogram. (IF 1.702) Pub Date : 2020-03-30 Lifei Zhang,Yebo Wang,Ye Zhang,Limengmeng Wang,He Huang
Factor-induced reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) as a powerful tool for regenerative medicine has gained wide attention in recent years. However, there are certain concerns regarding the efficiency of this reprogramming. Partially reprogrammed iPSCs (piPSCs) are stable cell lines originating from cells that have exited the normal reprogramming route at an early
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Yes-Associated Protein and PDZ Binding Motif: A Critical Signaling Pathway in the Control of Human Pluripotent Stem Cells Self-Renewal and Differentiation. Cell. Reprogram. (IF 1.702) Pub Date : 2020-03-30 Jia Shi,Maryam Farzaneh,Seyed Esmaeil Khoshnam
Human pluripotent stem cells (hPSCs) can self-renew indefinitely to generate cells like themselves with a normal karyotype and differentiate into other types of cells when stimulated with a proper set of internal and external signals. hPSCs including human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) are an alternative approach toward stem cell biology, drug discovery, disease
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Source and Follicular Fluid Treatment During the In Vitro Maturation of Recipient Oocytes Affects the Development of Cloned Pig Embryo. Cell. Reprogram. (IF 1.702) Pub Date : 2020-03-30 Huaxing Zhao,Shaoyi Xie,Ning Zhang,Zheng Ao,Xiao Wu,Liusong Yang,Junsong Shi,Ranbiao Mai,Enqin Zheng,Gengyuan Cai,Zhenfang Wu,Zicong Li
Pig cloning technique is valuable in agriculture, biomedicine, and life sciences. However, the full-term developmental efficiency of cloned pig embryos is only about 1%, which limits pig cloning application. The quality of recipient oocytes greatly affects the developmental competence of cloned pig embryos. Thus, this study investigated the effects of a recipient oocyte source (in vivo matured [IVVM]
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Inhibition of DNA Methyltransferase by RG108 Promotes Pluripotency-Related Character of Porcine Bone Marrow Mesenchymal Stem Cells. Cell. Reprogram. (IF 1.702) Pub Date : 2020-03-30 Qi Li,Yanhui Zhai,Xiaxia Man,Sheng Zhang,Xinglan An
Mesenchymal stem/stromal cells (MSCs) have been identified in almost all adult human tissues and been used in numerous clinical trials for a variety of diseases. Studies have shown that MSCs would undergo cellular senescence when cultured over a long term, which is brought on by increased epigenetic modifications, including DNA methylation. However, the mechanism of MSCs senescence is not well studied
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Reprogramming of Pluripotency-Specific microRNA Signatures Is Not Essential to Generate Inducible Pluripotent Stem Cells. Cell. Reprogram. (IF 1.702) Pub Date : 2020-02-01 Maria Skamagki,Cheng Zhang,Zhong Liu,Hu Li,Rui Zhao,Kitai Kim
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Effects of PXD101 and Embryo Aggregation on the In Vitro Development of Mouse Parthenogenetic Embryos. Cell. Reprogram. (IF 1.702) Pub Date : 2020-02-01 Xiaoyan Qiu,Xiong Xiao,Aoru Ren,Min Xiao,Haoyu Tian,Wenhui Ling,Mingyu Wang,Yuemin Li,Yongju Zhao
To improve the isolation efficiency of parthenogenetic embryonic stem cells (pESCs) in mice, it is necessary to optimize the method to increase in vitro developmental competence of mice parthenogenetic blastocysts. Therefore, this study aims to investigate an optimal method for the production of mouse parthenogenetic blastocysts and isolation of pESC colonies by comparing the effects of two methods:
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SQLE Promotes Differentiation and Apoptosis of Bovine Skeletal Muscle-Derived Mesenchymal Stem Cells. Cell. Reprogram. (IF 1.702) Pub Date : 2020-02-01 Ruimen Zhang,Yanfei Deng,Qiao Lv,Qinghua Xing,Yu Pan,Jingyuan Liang,Mingsheng Jiang,Yingming Wei,Deshun Shi,Bingkun Xie,Sufang Yang
In this study, Squalene epoxidase (SQLE) overexpression vector was transfected into bovine skeletal muscle-derived mesenchymal stem/stromal cells (MSCs) to study the molecular mechanism of SQLE regulating meat quality through myogenesis. We initially profiled the expression of SQLE in cattle embryos and adults, in the muscle tissue of four different cattle varieties, and in 11 different tissues/organs
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Rosuvastatin Attenuates Myocardial Ischemia-Reperfusion Injury via Upregulating miR-17-3p-Mediated Autophagy. Cell. Reprogram. (IF 1.702) Pub Date : 2019-11-15 Xiaoqin Wang,Jinghan Chen,Xiaojiao Huang
Myocardial diseases usually appear ischemic. Reperfusion therapy is one of the effective methods that can improve clinical therapeutic efficacy. However, reperfusion results in myocardial injury named I/R injury. Rosuvastatin (RS) is HMG-CoA reductase inhibitor. We investigated the role of RS in the myocardial I/R injury in vitro and its active mechanism. Oxygen-glucose deprivation/reoxygenation (OGD/R)
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In Vitro Induction of Human Embryonic Stem Cells into the Midbrain Dopaminergic Neurons and Transplantation in Cynomolgus Monkey. Cell. Reprogram. (IF 1.702) Pub Date : 2019-10-25 Xiaoyan Qiu,Yingquan Liu,Xiong Xiao,Jingjing He,Huiyun Zhang,Yuemin Li
A simple, rapid, efficient, and specialized culture system was successfully developed in this study to induce human embryonic stem cells into dopaminergic neurons in vitro. It only took 5 days to generate quickly and directly a large number of homogeneous neural stem cell (NSC) spheres by the introduction of small molecules LDN (inhibitor of BMP [bone morphogenetic protein] pathway that inhibits BMP
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Comparison of Carcass Traits, Meat Quality, and Chemical Composition of Tissues from Progeny Derived from Cloned and Noncloned Pigs. Cell. Reprogram. (IF 1.702) Pub Date : 2019-10-25 Ting Gu,Junsong Shi,Lvhua Luo,Zicong Li,Enqin Zheng,Gengyuan Cai,Linjun Hong,Zhenfang Wu
Systematic studies of progeny derived from somatic cell nuclear transferred animals in their biophysical and biochemical characters are critical in assessing the safety of this kind of food. In this study, we compared the carcass traits and meat quality of 12 cloned and noncloned pigs, respectively, and chemical composition of tissues of 6 cloned and noncloned pigs, respectively. The carcass trait
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Whole Blood Transcriptome Analysis for Lifelong Monitoring in Elite Sniffer Dogs Produced by Somatic Cell Nuclear Transfer. Cell. Reprogram. (IF 1.702) Pub Date : 2019-10-18 Sun-A Ock,Inchul Choi,Gi-Sun Im,Jae Gyu Yoo
Reproductive cloning by somatic cell nuclear transfer (SCNT) is a valuable method to propagate service dogs with desirable traits because of higher selection rates in cloned dogs. However, incomplete reprogramming is a major barrier to SCNT, and the assessment of reprogramming is limited to preimplantation embryos and tissues from dead and/or adult tissue. Thus, lifelong monitoring in SCNT dogs can
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Successful Derivation of an Induced Pluripotent Stem Cell Line from a Genetically Nonpermissive Enhanced Green Fluorescent Protein-Transgenic FVB/N Mouse Strain. Cell. Reprogram. (IF 1.702) Pub Date : 2019-10-01 Deepti Abbey,Gurbind Singh,Isha Verma,Suchitra Derebail,Udaykumar Kolkundkar,Darshan S Chandrashekar,Kshitish K Acharya,Mohan C Vemuri,Polani B Seshagiri
The embryonic stem cell line derivation from nonpermissive mouse strains is a challenging and highly inefficient process. The cellular reprogramming strategy provides an alternative route for generating pluripotent stem cell (PSC) lines from such strains. In this study, we successfully derived an enhanced green fluorescent protein (EGFP)-transgenic "N9" induced pluripotent stem cell (iPS cell, iPSC)
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DNA Methylation of the Insulin-Like Growth Factor 2-Imprinted Gene in Trophoblast Cells of Elongated Bovine Embryo: Effects of the In Vitro Culture. Cell. Reprogram. (IF 1.702) Pub Date : 2019-10-01 Anelise Dos Santos Mendonça,Maurício Machaim Franco,José de Oliveira Carvalho,Grazieli Marinheiro Machado,Margot Alves Nunes Dode
DNA methylation is an essential epigenetic mark for embryo development and can be susceptible to environment factors such as in vitro conditions. The aim of this study was to verify the effect of in vitro culture until Day (D) 14 of the development on the embryo size and DNA methylation pattern of the insulin-like growth factor 2 (IGF2)-imprinted gene. To achieve this, we produced bovine embryos completely
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Human Menstrual Blood Stem Cell-Derived Granulosa Cells Participate in Ovarian Follicle Formation in a Rat Model of Premature Ovarian Failure In Vivo. Cell. Reprogram. (IF 1.702) Pub Date : 2019-10-01 Parastoo Noory,Shadan Navid,Bagher Minaee Zanganeh,Ali Talebi,Maryam Borhani-Haghighi,Keykavos Gholami,Marjan Dehghan Manshadi,Mehdi Abbasi
We recently reported the application of human menstrual blood stem cells' (HuMenSCs) transplantation as a treatment modality in a rat model of premature ovarian failure (POF). We continued to investigate further in this respect. Female rats were injected intraperitoneally with 36 mg/kg busulfan. HuMenSCs were obtained, grown, and analyzed for immunophenotypic features at passage three. The cells were
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Partially Reprogrammed Induced Pluripotent Stem Cells Using MicroRNA Cluster miR-302s in Guangxi Bama Minipig Fibroblasts. Cell. Reprogram. (IF 1.702) Pub Date : 2019-09-03 Shuye Qiao,Yanfei Deng,Sheng Li,Xiaoling Yang,Deshun Shi,Xiangping Li
Pig-induced pluripotent stem cells (piPSCs) have great potential application in regenerative medicine. The miR-302s cluster alone has been shown to reprogram mouse and human somatic cells into induced pluripotent stem cells (iPSCs) without exogenous transcription factors. However, miR-302s alone have not been reported to reprogram cells in large livestock. In this study, we induced pig somatic cells
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Overexpression of MBD3 Improves Reprogramming of Cloned Pig Embryos. Cell. Reprogram. (IF 1.702) Pub Date : 2019-08-08 Xingwang Wang,Junsong Shi,Gengyuan Cai,Enqin Zheng,Dewu Liu,Zhenfang Wu,Zicong Li
Methyl-CpG-binding domain protein 3 (MBD3) is a core component of the nucleosome remodeling and deacetylase (NuRD) complex, which is crucial for pluripotent stem cell differentiation and embryonic development. MBD3 was shown to play important roles in transcription factor-induced somatic cell reprogramming. Expression level of MBD3 was demonstrated to be higher in somatic cell nuclear transfer-generated
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Generation of Induced Pluripotent Cancer Cells from Glioblastoma Multiform Cell Lines. Cell. Reprogram. (IF 1.702) Pub Date : 2019-08-01 Mousa Vatanmakanian,Hassan Yousefi,Ladan Mashouri,Amir Reza Aref,Gholamreza Khamisipour,Amirreza Bitaraf,Shaban Alizadeh
Generation of induced pluripotent stem cells (iPSCs) has been described as a powerful method to dedifferentiate the specialized cells to pluripotency. However, obtaining cancer-specific iPS cells (iPCs) encounters several barriers. The generation of iPCs provides valuable experimental platforms to mimic oncogenesis and offers potentials regarding drug screening. To overcome the difficulties regarding
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RepSox Increases Porcine Cloning Efficiency by Improving Pluripotency of Donor Nuclei. Cell. Reprogram. (IF 1.702) Pub Date : 2019-07-16 Guosong Qin,Jianguo Zhao,Jiaojiao Huang
Accumulating evidence suggests that a low pluripotency of donor nuclei might lead to abnormal development of cloned embryos and underlie the inefficiency of mammalian somatic cell nuclear transfer (SCNT). To improve the pluripotency of SCNT embryo, RepSox, a defined small-molecule compound that functions in inhibiting the transforming growth factor β signaling pathway, was used to treat nuclear-transferred
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Do the Cytoplast and Nuclear Material of Germinal Vesicle Oocyte Support Developmental Competence Upon Reconstruction with Embryonic/Somatic Nucleus. Cell. Reprogram. (IF 1.702) Pub Date : 2019-07-16 Abd El-Nasser Ahmed Mohammed,Saker Al-Suwaiegh,Tarek Al-Shaheen
Maturation conditions and oocytes quality have substantial roles on developmental competence of unreconstructed or reconstructed oocytes. Cloning has been reported successfully with low efficiency through embryonic or somatic nuclear transfer into enucleated metaphase II oocytes. It has been suggested that introducing embryonic or somatic nucleus to cytoplast at earlier stage might improve reprogramming
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Adipose-Derived Stem/Stromal Cells Recapitulate Aging Biomarkers and Show Reduced Stem Cell Plasticity Affecting Their Adipogenic Differentiation Capacity. Cell. Reprogram. (IF 1.702) Pub Date : 2019-07-12 Juliane-Susanne Jung,Christin Volk,Christina Marga,Alexander Navarrete Santos,Matthias Jung,Dan Rujescu,Anne Navarrete Santos
Stromal mesenchymal stem cells (MSCs) have the capability to self-renew and can differentiate into multiple cell types of the mesoderm germ layer, but their properties are affected by molecular aging mechanisms. MSCs can be obtained from adipose tissue termed as adipose-derived stem/stromal cells (ASCs) representing a promising tool for studying age-related diseases in detail. ASCs from young (16 weeks)
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Analysis of Human Stem Cell Transcription Factors. Cell. Reprogram. (IF 1.702) Pub Date : 2019-07-12 Smarakan Sneha,Rohit P Nagare,Pacharia Manasa,Sekar Vasudevan,Aboo Shabna,Trivadi Sundaram Ganesan
Transcription factors NANOG, OCT4, SOX2, and NESTIN are expressed in both human embryonic stem cells (hESCs) and cancer stem cells and they play a crucial role in maintaining characteristics of stemness such as self-renewal and pluripotency. This article evaluates the expression of variants of the main stem cell-specific transcription factors NANOG and OCT4 critically and accurately with specific primers
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Resveratrol Promotes Osteogenic Differentiation of Canine Bone Marrow Mesenchymal Stem Cells Through Wnt/Beta-Catenin Signaling Pathway. Cell. Reprogram. (IF 1.702) Pub Date : 2019-06-30 Xiao-E Zhao,Zhenshan Yang,Hui Zhang,Ge Yao,Jie Liu,Qiang Wei,Baohua Ma
Bone marrow mesenchymal stem cells (BMSCs) can transdifferentiate into different types of cells and may serve as a cell source for tissue engineering. Resveratrol has been shown to possess many benefits, including activation of osteogenesis. Furthermore, Wnt/β-catenin signaling has been known to promote osteogenic differentiation in many cells. In this study, we investigated the role of resveratrol
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Suprachoroidal Adipose Tissue-Derived Mesenchymal Stem Cell Implantation in Patients with Dry-Type Age-Related Macular Degeneration and Stargardt's Macular Dystrophy: 6-Month Follow-Up Results of a Phase 2 Study. Cell. Reprogram. (IF 1.702) Pub Date : 2019-06-30 Ayse Oner,Zeynep Burcin Gonen,Duygu Gülmez Sevim,Neslihan Smim Kahraman,Metin Unlu
This prospective clinical case series aimed to investigate the safety and efficacy of suprachoroidal adipose tissue-derived mesenchymal stem cell (ADMSC) implantation in patients with dry-type age-related macular degeneration (AMD) and Stargardt's macular dystrophy (SMD). This study included four patients with advanced-stage dry-type AMD and four patients with SMD who underwent suprachoroidal implantation
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Kidins220/ARMS Expression Confers Proliferation But Independent of Self-Renewal in Mouse Embryonic Stem Cells. Cell. Reprogram. (IF 1.702) Pub Date : 2019-06-30 Jingfeng Luo,Yang Zhou,Qi Chen,Xiaoli Zhao,Ming Zhang
Embryonic stem cells (ESCs) are characterized by their ability to self-renew and their potential to differentiate into any cell type. Therefore, identification of novel molecular markers to verify the pluripotent status of mouse ESCs (mESCs) is of great significance. Kinase D interacting substrate of 220 kDa (Kidins220)/ankyrin repeat-rich membrane spanning (ARMS) plays a crucial role in the integration
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Treatment of Buffalo (Bubalus bubalis) Somatic Cell Nuclear Transfer Embryos with MicroRNA-29b Mimic Improves Their Quality, Reduces DNA Methylation, and Changes Gene Expression Without Affecting Their Developmental Competence. Cell. Reprogram. (IF 1.702) Pub Date : 2019-06-14 Shikha Singh,Songyukta Shyam,Shrutika Sah,Manoj K Singh,Prabhat Palta
microRNA-29b (miR-29b) plays an important role in controlling DNA methylation in cells. We investigated its role during early embryonic development in buffalo embryos produced by somatic cell nuclear transfer (SCNT) and in vitro fertilization (IVF). miR-29b expression was highest at the 2-cell stage, decreased (p < 0.001) at the 4-cell stage, and remained low thereafter at the 8-cell, morula, and blastocyst
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Selection of Reference miRNAs for Relative Quantification in Buffalo (Bubalus bubalis) Blastocysts Produced by Hand-Made Cloning and In Vitro Fertilization. Cell. Reprogram. (IF 1.702) Pub Date : 2019-06-14 Swati Viviyan Lagah,Tanushri Jerath Sood,Prabhat Palta,Manishi Mukesh,Manmohan Singh Chauhan,Radhey Shyam Manik,Manoj Kumar Singh,Suresh Kumar Singla
Very low birth rate and a high incidence of abnormalities in offspring born from cloned embryos, which have limited the application of cloning technology on a wide scale, are believed to be because of incomplete or aberrant nuclear reprogramming. MicroRNAs (miRNAs) are involved in regulating a wide range of biological processes including reprogramming and embryonic development. Selection of suitable
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Effect of Periostin Silencing on the Autophagy of Osteoblasts. Cell. Reprogram. (IF 1.702) Pub Date : 2019-06-01 Han Qin,Jun Cai
The objective of the present work was to investigate the effect of Periostin (POSTN) silencing on autophagy in osteoblasts, and provide an experimental basis for studying the mechanism of dental eruption. The cells were divided into the following four groups according to their viral number: the NC group, pFU-GW-016PSC53349-1; group KD1, LVpFU-GW-016PSC66471-1; group KD2, LVpFU-GW-016PSC66472-1; and
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Curcumin Promotes Proliferation of Adult Neural Stem Cells and the Birth of Neurons in Alzheimer's Disease Mice via Notch Signaling Pathway. Cell. Reprogram. (IF 1.702) Pub Date : 2019-06-01 Jun Li,Yazhou Han,Mingduo Li,Caixia Nie
The abnormal deposition of amyloid-β peptide, a major component of senile plaques, has been reported to be the major cause of neuronal cell death and cognitive impairment in Alzheimer's disease (AD). Adult neurogenesis is related to the amelioration of impaired neurons and cognitive impairment. In the research, we investigated the function of curcumin on endogenous neural stem cells (NSCs) and hippocampal
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Preparation of Induced Pluripotent Stem Cells Using Human Peripheral Blood Monocytes. Cell. Reprogram. (IF 1.702) Pub Date : 2019-05-21 Sumito Isogai,Naoki Yamamoto,Noriko Hiramatsu,Yasuhiro Goto,Masamichi Hayashi,Masashi Kondo,Kazuyoshi Imaizumi
Since induced pluripotent stem (iPS) cells have been established, in recent years, clinical transplantation of cells differentiated from iPS cells derived from human skin fibroblasts is been in progress. On the contrary, monocytes have complete genome information without damage and gene recombination, they are contained in the peripheral blood by ∼3%-8% and differentiate into dendritic cells that are