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Low-Intensity Nanosecond Pulsed Electric Field Accelerates Osteogenic Transformation of Human Dermal Fibroblasts by Enhancing Cell Pluripotency. Cell. Reprogram. (IF 1.6) Pub Date : 2023-11-27 Jingtian Lai,Zewei Wang,Haiying Zhou,Pengfei Li,Hui Lu,Tian Tu
Autologous human fibroblasts have the potential to differentiate into the osteogenic lineage under specific conditions and can be utilized for bone regeneration. However, their efficiency is currently unsatisfactory. Recently, low-intensity nanosecond pulsed electric field (nsPEF) stimulation has been demonstrated to enhance cell pluripotency by activating epigenetic regulatory pathways. In this study
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Allogeneic Mesenchymal Stem Cells After In Vivo Transplantation: A Review. Cell. Reprogram. (IF 1.6) Pub Date : 2023-11-16 Derek B Asserson
Autologous mesenchymal stem cells (MSCs) are ideal for tissue regeneration because of their ability to circumvent host rejection, but their procurement and processing present logistical and time-sensitive challenges. Allogeneic MSCs provide an alternative cell-based therapy capable of positively affecting all human organ systems, and can be readily available. Extensive research has been conducted in
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Microscopic Analysis of Cell Fate Alteration Induced by Cell Fusion. Cell. Reprogram. (IF 1.6) Pub Date : 2023-10-01 Taisei Kumazaki,Chinatsu Yonekawa,Tomomi Tsubouchi
In mammals, differentiated cells generally do not de-differentiate nor undergo cell fate alterations. However, they can be experimentally guided toward a different lineage. Cell fusion involving two different cell types has long been used to study this process, as this method induces cell fate alterations within hours to days in a subpopulation of fused cells, as evidenced by changes in gene-expression
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Reprogramming Cell Identity: Past Lessons, Challenges, and Future Directions. Cell. Reprogram. (IF 1.6) Pub Date : 2023-10-01 José C R Silva
Reprogramming is traditionally defined as the fate conversion of a cell to a stage of increased developmental potential. In its broader meaning, the reprogramming term is also applied to all forms of cell fate conversion that do not follow a developmental trajectory. Reprogramming is now a well-established field of research that gained rapid progress upon the advent of induced pluripotency. In this
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Pioneer of Cloning and Inspirational Figure for Cellular Reprogramming Scientists Sir Ian Wilmut (July 7, 1944-September 10, 2023). Cell. Reprogram. (IF 1.6) Pub Date : 2023-10-01 Carlos-Filipe Pereira
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Induced Pluripotent Stem Cell-Derived Chimeric Antigen Receptor T Cells: The Intersection of Stem Cells and Immunotherapy. Cell. Reprogram. (IF 1.6) Pub Date : 2023-09-29 Mohammad Reza Lahimchi,Faezeh Maroufi,Amirhosein Maali
Chimeric antigen receptor (CAR) T cell therapy is a promising cell-based immunotherapy applicable to various cancers. High cost of production, immune rejection, heterogeneity of cell product, limited cell source, limited expandability, and relatively long production time have created the need to achieve a universal allogeneic CAR-T cell product for "off-the-shelf" application. Since the innovation
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Hepatitis B Virus x Protein Increases Cellular OCT3/4 and MYC and Facilitates Cellular Reprogramming. Cell. Reprogram. (IF 1.6) Pub Date : 2023-09-26 Madhusudana Girija Sanal,Sarita Gupta,Rahul Saha,Nisha Vats,Shiv Kumar Sarin
Hepatitis B virus x (HBx) is a multifunctional protein coded by the Hepatitis B virus that is involved in various cellular processes such as proliferation, cell survival/apoptosis, and histone methylation. HBx was reported to be associated with liver "cancer stem cells." The stemness inducing properties of HBx could also facilitate the generation of pluripotent stem cells from somatic cells. It is
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Efficient A·T-to-C·G Base Editing via Adenine Transversion Editors. Cell. Reprogram. (IF 1.6) Pub Date : 2023-09-19 Muhammad Arslan Mahmood
Generating A-to-C transversions to correct defective alleles or introduce novel alleles has posed significant challenges. However, two recent studies focusing on adenine transversions have achieved successful A-to-C transversions in mouse embryos and plant cell. These remarkable accomplishments notably broaden the range of base editing and their applications both in fundamental research and in therapeutics
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Proteomic Analysis and Reprogramming Potential of the Porcine Intra-Ooplasmic Nanovesicles. Cell. Reprogram. (IF 1.6) Pub Date : 2023-09-19 Islam M Saadeldin,Seonggyu Bang,Abdulkadir Y Maigoro,Sung Ho Yun,Seung Ii Kim,Sanghoon Lee,Jongki Cho
Oocytes contain reprogramming machinery that can transform somatic cells into totipotent cells. In this study, we aimed to isolate and characterize nanovesicles from mature porcine oocytes and described them for the first time as "intra-ooplasmic vesicles (IOVs)". Isolated IOVs had an average diameter of 186.3 ± 10.8 nm. Proteomic analysis revealed 467 peptide reads, with the top 20 proteins related
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SIRT6 Knockdown in Buffalo Fetal Fibroblasts Exacerbates Premature Senescence Caused by DNA and Telomere Damage. Cell. Reprogram. (IF 1.6) Pub Date : 2023-09-19 Jingyuan Liang,Jiayu Cui,Juanru Cheng,Yu Pan,Ruimen Zhang,Sufang Yang,Lingxiu Zou
As a gene with antiaging functions, sirtuin6 (SIRT6) belonging to the sirtuin family plays a vital role in DNA repair, telomerase function, and cellular senescence, as well as maintains epigenomic stability and promotes longevity. However, its role in cell senescence in large animals, such as buffaloes, remains unknown. Fibroblasts are commonly used for somatic reprogramming, and their physiological
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Cell Reprogramming Techniques: Contributions to Cancer Therapy. Cell. Reprogram. (IF 1.6) Pub Date : 2023-08-03 Tongtong Guo,Qi Wei
The reprogramming of terminally differentiated cells over the past few years has become important for induced pluripotent stem cells (iPSCs) in the field of regenerative medicine and disease drug modeling. At the same time, iPSCs have also played an important role in human cancer research. iPSCs derived from cancer patients can be used to simulate the early progression of cancer, for drug testing,
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A Novel Method for Human Adipose-Derived Stem Cell Isolation and Cryopreservation. Cell. Reprogram. (IF 1.6) Pub Date : 2023-08-01 Young-Cheol Lim,Jung-Il Jung,In-Kee Hong
Adipose-derived stem cells (ADSCs) are isolated from abundant adipose tissue and have the capacity to differentiate into multiple cell lineages. ADSCs have raised big interest in therapeutic applications in regenerative medicine and demonstrated to fulfill the criteria for a successful cell therapy. There are several methods for isolation of ADSCs from adipose tissue and cryopreservation of ADSCs.
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Reprogramming Stars #13: Establishing Connections with Cellular Reprogramming-An Interview with Dr. Daniella Rylander Ottosson. Cell. Reprogram. (IF 1.6) Pub Date : 2023-08-01 Daniella Rylander Ottosson,Carlos-Filipe Pereira
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Unleashing Ascl1: Exploring Cross-Lineage Potential in Reprogramming and Regenerative Frontiers. Cell. Reprogram. (IF 1.6) Pub Date : 2023-08-01 Camila Vazquez Echegaray
In the era of stem cell research and regenerative medicine, understanding the regulatory networks that drive cellular reprogramming is fundamental. The study entitled "Cross-lineage potential of Ascl1 uncovered by comparing diverse reprogramming regulatomes" published in Stem Cell Reports sheds light on the remarkable versatility of Ascl1, a transcription factor known for its pivotal role in neurogenesis
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Cell Transdifferentiation: A Challenging Strategy with Great Potential. Cell. Reprogram. (IF 1.6) Pub Date : 2023-07-20 Fuping Wang,Runting Li,Limeng Zhang,Xiaoning Nie,Linqing Wang,Longxin Chen
With the discovery and development of somatic cell nuclear transfer, cell fusion, and induced pluripotent stem cells, cell transdifferentiation research has presented unique advantages and stimulated a heated discussion worldwide. Cell transdifferentiation is a phenomenon by which a cell changes its lineage and acquires the phenotype of other cell types when exposed to certain conditions. Indeed, many
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Transcriptomic Heterogeneity of Human Mesenchymal Stem Cells Derived from Bone Marrow, Dental Pulp, Adipose Tissue, and Umbilical Cord. Cell. Reprogram. (IF 1.6) Pub Date : 2023-06-29 Xiaoxiao Zhu,Xinchen Xu,Mengyuan Shen,Yingying Wang,Tao Zheng,Huitao Li,Xing Wang,Jian Meng
Compared with mesenchymal stem cells (MSCs) obtained from other tissue sources, those derived from umbilical cord (UC) tissue exhibit numerous advantages and vast potential for therapeutic applications. However, MSCs from different tissue sources are heterogeneous, and therefore, the therapeutic efficacy of UC-derived MSCs as a replacement for other tissue-derived MSCs needs to be studied. To better
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Human Induced Pluripotent Stem Cell-Derived Pericytes as Scalable and Editable Source to Study Direct Lineage Reprogramming Into Induced Neurons. Cell. Reprogram. (IF 1.6) Pub Date : 2023-06-27 Radhika Menon,Linda Petrucci,Benjamin Lohrer,Jingzhong Zhang,Markus Schulze,Christian Schichor,Beate Winner,Jürgen Winkler,Markus J Riemenschneider,Ralf Kühn,Sven Falk,Marisa Karow
Studying human somatic cell-to-neuron conversion using primary brain-derived cells as starting cell source is hampered by limitations and variations in human biopsy material. Thus, delineating the molecular variables that allow changing the identity of somatic cells, permit adoption of neuronal phenotypes, and foster maturation of induced neurons (iNs) is challenging. Based on our previous results
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A Safer Path to Cellular Rejuvenation: Endogenous Oct4 Activation via CRISPR/dCas9 in Progeria Mouse Models. Cell. Reprogram. (IF 1.6) Pub Date : 2023-06-16 Di Hu,Enora Le Borgne,Rico Meinl
A recent study in Aging Cell showed that transcriptional activation of endogenous Oct4 using the CRISPR/dCas9 activator system is sufficient for cellular rejuvenation and extending the lifespan of a progeria mouse model. Although transient expression of reprogramming factors Oct4, Sox2, Klf4, and c-Myc (OSKM) has been shown to ameliorate age-related phenotypes in vivo, oncogenic risk, for example,
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Reprogramming Stars #12: At the Heart of In Vivo Reprogramming- An Interview with Dr. Li Qian. Cell. Reprogram. (IF 1.6) Pub Date : 2023-06-01 Li Qian,Carlos-Filipe Pereira
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MicroRNA26a Overexpression Hastens Osteoblast Differentiation Capacity in Dental Stem Cells. Cell. Reprogram. (IF 1.6) Pub Date : 2023-05-18 Steven Kaufman,Peter Chang,Elisha Pendleton,Nalini Chandar
Dental pulp stem cells (DPSCs) and stem cells from human exfoliated deciduous teeth (SHED) are a source of mesenchymal stem cells with the potential to differentiate into several cell types. We initially isolated SHED cells and compared their osteogenic capacity with commercially available DPSCs. Both cells exhibited similar capacities of growth and osteogenic differentiation. A fourfold to sixfold
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Ameliorative Effects of Extracellular Vesicles Derived from Mesenchymal Stem Cells on Apoptosis and Differentiation of Osteoblasts Treated with CoCl2. Cell. Reprogram. (IF 1.6) Pub Date : 2023-05-15 Qicheng Li,Wei Zhang,Jin Deng,Qiuya Li,Xiaoyang Fu,Yuhui Kou,Na Han
Severe osteoporotic fracture occurring in sites with inadequate blood supply can cause irreversible damage to cells, particularly osteoblasts, with current drug and surgical interventions exhibiting limitations for elderly individuals. As participants mediating intercellular communication, extracellular vesicles (EVs) are rarely reported to play functional roles in osteoblasts under hypoxia. Our study
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Functions of Key Enzymes of Glycolytic Metabolism in Tumor Microenvironment. Cell. Reprogram. (IF 1.6) Pub Date : 2023-05-12 Wenxin Xu,Jialei Weng,Minghao Xu,Qiang Zhou,Shaoqing Liu,Zhiqiu Hu,Ning Ren,Chenhao Zhou,Yinghao Shen
The tumor microenvironment (TME) plays a crucial role in tumor initiation, growth and metastasis. Metabolic enzymes involved in tumor glycolytic reprogramming, including hexokinase, pyruvate kinase, and lactate dehydrogenase, not only play key roles in tumorigenesis and maintaining tumor cell survival, but also take part in the modulation of the TME. Many studies have been devoted to the role of key
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The Emerging Role of B1 SINE in Pluripotent Reprogramming. Cell. Reprogram. (IF 1.6) Pub Date : 2023-05-08 Jere Weltner,Ras Trokovic
By screening a CRISPR knockout library for mouse pluripotent reprogramming roadblock genes, Kaemena et al. identify the KRAB-ZFP factor Zfp266 as a suppressor of efficient reprogramming. Furthermore, by analyzing DNA binding and chromatin openness, the authors found that ZFP266 has a role in suppressing reprogramming by targeting the B1 SINE sequences for silencing.
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Production of MSTN Gene-Edited Embryos of Buffalo Using the CRISPR/Cas9 System and SCNT. Cell. Reprogram. (IF 1.6) Pub Date : 2023-04-11 Seema Dua,Sonu Bansal,Devika Gautam,Bosco Jose,Priyanka Singh,Manoj Kumar Singh,Sachinandan De,Dharmendra Kumar,Prem Singh Yadav,Wilfried Kues,Naresh L Selokar
The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and somatic cell nuclear transfer (SCNT) have been used to produce genome-edited farm animal species for improved production and health traits; however, these tools are rarely used in the buffalo and can play a pivotal role in milk and meat production in tropical and subtropical countries. In this study, we aimed to
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The Emerging Biological Functions of Exosomes from Dental Tissue-Derived Mesenchymal Stem Cells. Cell. Reprogram. (IF 1.6) Pub Date : 2023-04-01 Shu Ma,Yidi Jiang,Yuyan Qian,Jing Du,Xiaoyan Yu,Shiyi Luo,Zhu Chen
Exosomes are one kind of small-cell extracellular membranous vesicles that can regulate intercellular communication and give rise to mediating the biological behaviors of cells, involving in tissue formation, repair, the modulation of inflammation, and nerve regeneration. The abundant kinds of cells can secret exosomes, among them, mesenchymal stem cells (MSCs) are very perfect cells for mass production
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Reprogramming Stars #11: Teaming Up to Uncover the Epitranscriptomics of Reprogramming-An Interview with Dr. Miguel Fidalgo and Dr. Diana Guallar. Cell. Reprogram. (IF 1.6) Pub Date : 2023-04-01 Miguel Fidalgo,Diana Guallar,Carlos-Filipe Pereira
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Treatments of Porcine Nuclear Recipient Oocytes and Somatic Cell Nuclear Transfer-Generated Embryos with Various Reactive Oxygen Species Scavengers Lead to Improvements of Their Quality Parameters and Developmental Competences by Mitigating Oxidative Stress-Related Impacts. Cell. Reprogram. (IF 1.6) Pub Date : 2023-03-20 Seung-Hwan Oh,Seung-Eun Lee,Dong-Hun Han,Jae-Wook Yoon,So-Hee Kim,Eun-Seo Lim,Han-Bi Lee,Eun-Young Kim,Se-Pill Park
This study investigated the antioxidant effects of β-cryptoxanthin (BCX), hesperetin (HES), and icariin (ICA), and their effects on in vitro maturation of porcine oocytes and subsequent embryonic development of somatic cell nuclear transfer (SCNT). Treatment with 1 μM BCX (BCX-1) increased the developmental rate of porcine oocytes more than treatment with 100 μM HES (HES-100) or 5 μM ICA (ICA-5). The
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Evaluation of All Human Transcription Factors on the Directed Differentiation of Pluripotent Stem Cells. Cell. Reprogram. (IF 1.6) Pub Date : 2023-03-16 Rodrigo L Dos Santos
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A Fundamental Research in In Vitro Spermatogonial Stem Cell Culturing: What Are Clump Cells? Cell. Reprogram. (IF 1.6) Pub Date : 2023-02-27 Kiana Sojoudi,Hossein Azizi,Thomas Skutella
Spermatogonial stem cells (SSCs) are a small group of testicular cells located in the basement membrane of seminiferous tubules and can balance self-renewal and differentiation during spermatogenesis. Our in vitro culture experiments of mouse SSCs indicated heterogeneity of cultured cells. Highly compact colonies were observed next to SSC colonies, which we call clump cells. We used immunocytochemical
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The Wisdom in Teeth: Neuronal Differentiation of Dental Pulp Cells Cell. Reprogram. (IF 1.6) Pub Date : 2023-01-31 Bendegúz Sramkó, Anna Földes, Kristóf Kádár, Gábor Varga, Ákos Zsembery, Karolina Pircs
Mesenchymal stem/stromal cells (MSCs) are found in almost all postnatal organs. Under appropriate environmental cues, multipotency enables MSCs to serve as progenitors for several lineage-specific, differentiated cell types. In vitro expansion and differentiation of MSCs give the opportunity to obtain hardly available somatic cells, such as neurons. The neurogenic potential of MSCs makes them a promising
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Reprogramming Stars #10: Modeling Cancer with Cellular Reprogramming-An Interview with Dr. Dung-Fang Lee. Cell. Reprogram. (IF 1.6) Pub Date : 2023-01-31 Dung-Fang Lee,Carlos-Filipe Pereira
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Roadmap of the Early Events of In Vivo Somatic Cell Reprogramming Cell. Reprogram. (IF 1.6) Pub Date : 2023-01-25 Diana Guallar
Single-cell transcriptomics and in situ imaging of murine pancreas upon partial reprogramming in vivo reveal transcriptional dynamics upon Oct4, Sox2, Klf4, and cMyc (OSKM) induction. Interestingly, transcriptomic signatures of partial reprogramming observed in pancreas are shared by several tissues upon OSKM induction as well as during in vitro reprogramming of fibroblasts, pointing to the existence
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The Role of Histone Acetylation Modification in Dental Tissue-Derived Mesenchymal Stem Cells and Odontogenesis Cell. Reprogram. (IF 1.6) Pub Date : 2022-12-30 Haoling Chen, Zijing Huang, Chuxiao Chen
Odontogenesis is a complex physiological process that is based on dental tissue-derived mesenchymal stem cells (MSCs). Dental tissue-derived MSCs are the stem cell populations isolated and characterized from different parts of the oral cavity, and are considered as promising candidates for stem cell-based therapy. During odontogenesis, epigenetic factors can influence the proliferation, differentiation
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Advances in Understanding the Roles of Mesenchymal Stem Cells in Lung Cancer Cell. Reprogram. (IF 1.6) Pub Date : 2022-12-30 Wenli Ding, Kexin Zhang, Qinying Li, Linfei Xu, Yanhui Ma, Fang Han, Liang Zhu, Xiaodong Sun
Lung cancer is the most common and deadliest type of cancer worldwide. Research concerning lung cancer has made considerable progress in recent decades, but lung cancer remains the leading cause of malignancy-related mortality rate. Mesenchymal stem cells (MSCs) mainly exist in fat, umbilical cord blood, bone marrow, bone, and muscle. MSCs are a primary component of the tumor microenvironment (TME)
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Early Life Reprogramming-Based Treatment Promotes Longevity Cell. Reprogram. (IF 1.6) Pub Date : 2022-12-30 Patrizia Pessina, Bruno Di Stefano
Short-term expression of Yamanaka factors early in life promotes epigenetic reprogramming and an increased healthy lifespan in a mouse model of accelerated aging.
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Bcl11b and Atoh8 Coordinate Cellular Plasticity for Reprogramming and Transformation Cell. Reprogram. (IF 1.6) Pub Date : 2022-12-12 Mo-Fan Huang, Rachel Shoemaker, Dung-Fang Lee
By dissecting and comparing the transcriptional trajectories and epigenomic traits of reprogramming and transforming cells at the single-cell resolution, Huyghe et al discovered Bcl11b and Atoh8, two key transcription factors controlling cell plasticity during pluripotent reprogramming and oncogenic transformation.
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The Therapeutic Potential of Mesenchymal Stem Cells in the Treatment of Diabetes Mellitus Cell. Reprogram. (IF 1.6) Pub Date : 2022-12-12 Liang Zhu, Sheng Wang, JunSheng Qu, Zongguang Hui, Chengxia Kan, Ningning Hou, Xiaodong Sun
Mesenchymal stem cells (MSCs) exist in many tissues and can differentiate into cells of multiple lineages, such as adipocytes, osteoblasts, or chondrocytes. MSC administration has demonstrated therapeutic potential in various degenerative and inflammatory diseases (e.g., graft-vs.-host disease, multiple sclerosis, Crohn's disease, organ fibrosis, and diabetes mellitus [DM]). The mechanisms involved
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The Tumor Microenvironment Reprograms Immune Cells Cell. Reprogram. (IF 1.6) Pub Date : 2022-12-12 Handi Cao, Sanxing Gao, Ritika Jogani, Ryohichi Sugimura
Tumor tissue comprises a highly complex network of diverse cell types. The tumor microenvironment (TME) can be mainly subdivided into cancer cells and stromal cell compartments, the latter include different types of immune cells, fibroblasts, endothelial cells, and pericytes. Tumor cells reprogram immune cells and other stromal cells in the TME to constrain their antitumor capacity by creating an immunosuppressive
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Fluorescent Reporters Distinguish Stem Cell Colony Subtypes During Somatic Cell Reprogramming Cell. Reprogram. (IF 1.6) Pub Date : 2022-12-12 Alexandra Moauro, Robin E. Kruger, Daniel O'Hagan, Amy Ralston
Somatic cell reprogramming was first developed to create induced pluripotent stem (iPS) cells. Since that time, the highly dynamic and heterogeneous nature of the reprogramming process has come to be appreciated. Remarkably, a distinct type of stem cell, called induced extraembryonic endoderm (iXEN) stem cell, is also formed during reprogramming of mouse somatic cells by ectopic expression of the transcription
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Reprogramming Stars #9: Spacing Out Cellular Reprogramming-An Interview with Dr. Valentina Fossati. Cell. Reprogram. (IF 1.6) Pub Date : 2022-11-21 Valentina Fossati,Carlos-Filipe Pereira
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Direct Reprogramming Retains Aging Signatures That Are Critical to Reveal Parkinson's Disease-Associated Autophagy Phenotypes. Cell. Reprogram. (IF 1.6) Pub Date : 2022-11-17 Elezabeth Stephen,Heather Mortiboys
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Call for Special Issue Papers: Cellular Reprogramming 25th Anniversary Deadline for Manuscript Submission: April 30, 2023. Cell. Reprogram. (IF 1.6) Pub Date : 2022-11-14 Carlos-Filipe Pereira
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Past, Present, and Future of Direct Cell Reprogramming Cell. Reprogram. (IF 1.6) Pub Date : 2022-10-07 Henrik Ahlenius
Budding off from the broader developmental biology and stem cell research fields, cellular reprogramming is now established as a prominent discipline in its own right. Direct cell reprogramming is defined as the cell fate conversion of a somatic cell toward another identity without a pluripotent intermediate state. In addition to the opportunity for mechanistic dissection of lineage commitment in human
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The 2022 International Society for Stem Cell Research (ISSCR) Annual Meeting: Celebrating 20 Years of Achievements Cell. Reprogram. (IF 1.6) Pub Date : 2022-10-07 Janelle Drouin-Ouellet, Dan Li, Yuancheng Ryan Lu, Camila Vazquez Echegaray
Last June, the stem cell community came together to celebrate the 20th anniversary of the International Society for Stem Cell Research (ISSCR), one of the leading organizations in the field. The hybrid event mixed a varied program filled with plenary talks, concurrent track sessions, poster presentations, exhibit booths, and plenty of opportunities to enhance stem cell research through bonding between
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Synthetic Embryos Can Complete Gastrulation and Initiate Organogenesis Ex Utero Cell. Reprogram. (IF 1.6) Pub Date : 2022-10-07 Alejo E. Rodriguez-Fraticelli
Developmental biology has been revolutionized by two recent articles showing that synthetic mouse embryos derived from embryonic stem cells (ESCs) can be grown ex vivo and complete gastrulation up to the organogenesis stage. This is a remarkable achievement that had never been attained using stem cells before. Both studies used transcription factors to reprogram extraembryonic cells, which they combined
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Small Molecules Pushing Erythroid/Megakaryocyte Cell Specification Boundaries Cell. Reprogram. (IF 1.6) Pub Date : 2022-10-07 Johan Flygare
A combination of a MEK/ERK-signaling inhibitor and three chromatin-remodeling molecules enhances generation of platelet-producing megakaryocytes in vitro, possibly through direct reprogramming.
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Disease Modeling of Neurodegenerative Disorders Using Direct Neural Reprogramming Cell. Reprogram. (IF 1.6) Pub Date : 2022-10-07 Emilie M. Legault, Julie Bouquety, Janelle Drouin-Ouellet
Understanding the pathophysiology of CNS-associated neurological diseases has been hampered by the inaccessibility of patient brain tissue to perform live analyses at the molecular level. To this end, neural cells obtained by differentiation of patient-derived induced pluripotent stem cells (iPSCs) are considerably helpful, especially in the context of monogenic-based disorders. More recently, the
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Direct Reprogramming of Different Cell Lineages into Pancreatic β-Like Cells Cell. Reprogram. (IF 1.6) Pub Date : 2022-10-07 Jonathan L. Colarusso, Qiao Zhou
One major goal of regenerative medicine is the production of pancreatic endocrine islets to treat insulin-dependent diabetic patients. Among the different methods developed to achieve this goal, a particularly promising approach is direct lineage reprogramming, in which non-β-cells are directly converted to glucose-responsive, insulin-secreting β-like cells. Efforts by different research groups have
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Direct Cell Conversion of Somatic Cells into Dopamine Neurons: Achievements and Perspectives Cell. Reprogram. (IF 1.6) Pub Date : 2022-10-07 Simona Aversano, Renata Palladino, Massimiliano Caiazzo
In the last decade, direct reprogramming has emerged as a novel strategy to obtain mature and functional dopamine neurons from somatic cells. This approach could overcome issues linked to the use of human pluripotent stem cells such as ethical concerns and safety problems that can arise from the overgrowth of undifferentiated cells after transplantation. Several conversion methodologies have been developed
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Direct Reprogramming of Mice Skin Fibroblasts into Insulin-Producing Cells In Vitro Cell. Reprogram. (IF 1.6) Pub Date : 2022-10-07 Israa S. Salman, Ahmed Majeed Al-Shammari, Mukhtar Khamis Haba
Transdifferentiation means mature cell conversion into other mature cells. Ethical issues, epigenetic failure, or teratoma development are found in cellular reprogramming strategies. Thus, new methods are needed. This study aimed to develop a new novel formula of chemical molecules and growth factors that differentiate skin fibroblasts into insulin-producing cells (IPCs). Newborn mice fibroblasts differentiated
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Appropriate Exogenous Expression Stoichiometry of GATA4 as an Important Factor for Cardiac Reprogramming of Human Dermal Fibroblasts Cell. Reprogram. (IF 1.6) Pub Date : 2022-10-07 Xiangyu Zhang, Qi Zhang, Lijun Chen, Baomei Cai, Mengying Zeng, Sihua Ou, Yating Chen, Ziyu Feng, Huan Chen, Shangtao Cao, Kai Kang
Reprogramming of human dermal fibroblasts (HDFs) into induced cardiomyocyte-like cells (iCMs) represents a promising strategy for human cardiac regeneration. Different cocktails of cardiac transcription factors can convert HDFs into iCMs, although with low efficiency and immature phenotype. Here, GATA4, MEF2C, TBX5, MESP1, and MYOCD (GMTMeMy for short) were used to reprogram HDFs by retrovirus infection
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Modeling Mitochondrial Encephalomyopathy, Lactic Acidosis, and Stroke-Like Episodes Syndrome Using Patient-Derived Induced Neurons Generated by Direct Reprogramming Cell. Reprogram. (IF 1.6) Pub Date : 2022-10-07 Suleva Povea-Cabello, Marina Villanueva-Paz, Irene Villalón-García, Marta Talaverón-Rey, Mónica Álvarez-Cordoba, Juan M. Suárez-Rivero, María Ángeles Montes, Antonio Rodríguez-Moreno, Yuniesky Andrade-Talavera, José A. Armengol, José A. Sánchez-Alcázar
Mitochondrial diseases are a heterogeneous group of rare genetic disorders caused by mutations in nuclear or mitochondrial DNA (mtDNA). These diseases are frequently multisystemic, although mainly affect tissues that require large amounts of energy such as the brain. Mutations in mitochondrial transfer RNA (mt-tRNA) lead to defects in protein translation that may compromise some or all mtDNA-encoded
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Chemical Replacement of Noggin with Dorsomorphin Homolog 1 for Cost-Effective Direct Neuronal Conversion Cell. Reprogram. (IF 1.6) Pub Date : 2022-10-07 Lena Böhnke, Lucia Zhou-Yang, Silvia Pelucchi, Flora Kogler, Daniela Frantal, Florian Schön, Stina Lagerström, Oliver Borgogno, Jennifer Baltazar, Joseph R. Herdy, Sarah Kittel-Schneider, Michaela Defrancesco, Jerome Mertens
The direct conversion of adult human skin fibroblasts (FBs) into induced neurons (iNs) represents a useful technology to generate donor-specific adult-like human neurons. Disease modeling studies rely on the consistently efficient conversion of relatively large cohorts of FBs. Despite the identification of several small molecular enhancers, high-yield protocols still demand addition of recombinant
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An Improved Method to Generate Human Induced Astrocytes Cell. Reprogram. (IF 1.6) Pub Date : 2022-08-12 Janelle Drouin-Ouellet
A major improvement in the generation of astrocytes directly from human fibroblasts will now facilitate the study of how aging impacts on astrocyte function and whether this contributes to neurodegenerative disorders.
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Signaling Pathways and Protein–Protein Interaction of Vimentin in Invasive and Migration Cells: A Review Cell. Reprogram. (IF 1.6) Pub Date : 2022-08-12 Danial Hashemi Karoii, Hossein Azizi, Mahdi Amirian
The vimentin (encoded by VIM) is one of the 70 human intermediate filaments (IFs), building highly dynamic and cell-type-specific web networks in the cytoplasm. Vim−/− mice exhibit process defects associated with cell differentiation, which can have implications for understanding cancer and disease. This review showed recent reports from studies that unveiled vimentin intermediate filaments (VIFs)
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Amphiregulin Supplementation During Pig Oocyte In Vitro Maturation Enhances Subsequent Development of Cloned Embryos by Promoting Cumulus Cell Proliferation Cell. Reprogram. (IF 1.6) Pub Date : 2022-08-12 Xianjun Zhang, Huaxing Zhao, Yanan Li, Yuxing Zhang, Yalin Liang, Junsong Shi, Rong Zhou, Linjun Hong, Gengyuan Cai, Zhenfang Wu, Zicong Li
The oocyte in vitro maturation (IVM) technique is important in animal husbandry, biomedicine, and human-assisted reproduction. However, the developmental potential of in vitro matured oocytes is usually lower than that of in vivo matured (IVVM) oocytes. Amphiregulin (AREG) is an EGF-like growth factor that plays critical roles in the maturation and development of mammalian oocytes. This study investigated
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In Vitro Induction of Human Dental Pulp Stem Cells to Lymphatic Endothelial Cells Cell. Reprogram. (IF 1.6) Pub Date : 2022-08-12 Shuqun Qi, Li Ye, Liru Hu, Jian Pan
Lymphedema is a progressive and irreversible disease due to the lymphatic system disorder. Conservative and surgical therapies are either ineffective or impractical. Currently, mesenchymal stem cells (MSCs)-based therapies seem to be the most promising treatment for lymphedema. The MSCs promote lymphangiogenesis through the paracrine approach or by directly differentiating into lymphatic endothelial
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Deducing Insulin-Producing Cells from Goat Adipose Tissue-Derived Mesenchymal Stem Cells Cell. Reprogram. (IF 1.6) Pub Date : 2022-08-12 Amit Dubey, Sikander Saini, Vishal Sharma, Hrudananda Malik, Dinesh Kumar, Arun Kumar De, Debasis Bhattacharya, Dhruba Malakar
Mesenchymal stem cell is a potent tool for regenerative medicine against control of incurable diseases in human and animals. Diabetes mellitus is one such condition marked with the blood glucose is high due to lack of insulin (INS) hormone secreted by the pancreatic cells. Rare, but sporadic, cases of dysfunctional pancreatic cells in goat as well as the promises of stem cell therapy as an off-the-shelf
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Reprogramming Stars #8: A Synthetic Biology Approach to Cellular Reprogramming-An Interview with Dr. Katie Galloway. Cell. Reprogram. (IF 1.6) Pub Date : 2022-07-27 Kate E Galloway,Carlos-Filipe Pereira
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An Alternative Way to Improve Mammalian Embryo Development In Vitro: Culture of Zona Pellucida-Free Embryos Cell. Reprogram. (IF 1.6) Pub Date : 2022-06-10 Sarah Madani, Zoltan Machaty, Gábor Vajta
An increasing number of data proves that the presence of the zona pellucida is not essential to mammalian embryo production, including maturation, fertilization, and embryo culture. In fact, the structure of the zona pellucida of in vitro-produced embryos differs significantly from its in vivo counterpart, influencing metabolism and requiring disproportionate efforts to crack open at the time of hatching