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Role of Histamine H3 Receptor Antagonist Pitolisant in Early Neural Differentiation of Mouse Embryonic Stem Cells. Stem Cells Dev. (IF 4.0) Pub Date : 2023-11-30 Genghua Xu,Nuoya Liu,Yaqing Qiu,Jiayu Qi,Danyan Zhu
The histamine H3 receptor, prominently expressed in neurons with a minor presence in glial cells, acts as both an autoreceptor and an alloreceptor, controlling the release of histamine and other neurotransmitters. The receptor impacts various essential physiological processes. Our team's initial investigations had demonstrated that the histamine H3 receptor antagonists could facilitate the nerve regeneration
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Leveraging Temporal Wnt Signal for Efficient Differentiation of Intestinal Stem Cells in an Organoid Model. Stem Cells Dev. (IF 4.0) Pub Date : 2023-11-20 Li Yang,Xulei Wang,Guoqing Zhao,Liling Deng,Xiaolei Yin
The homeostasis of the intestinal epithelium heavily relies on the self-renewal and differentiation of intestinal stem cells (ISCs). Although the orchestration of these processes by signaling pathways such as the Wnt, BMP, Notch, and MAPK signals has been extensively studied, the dynamics of their regulation remains unclear. Our study explores how the Wnt signaling pathway temporally regulates the
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Single-Cell RNA-Seq Analysis Identifies Angiotensinogen and Galanin as Unique Molecular Markers of Acinar Cells in Murine Salivary Glands. Stem Cells Dev. (IF 4.0) Pub Date : 2023-11-16 Jingming Liu,Yanan Li,Yuxin Zhang,Qianyu Cheng,Huikai Liu,Liwen He,Liang Chen,Tianyu Zhao,Panpan Liang,Wenping Luo
The submandibular gland (SMG) and sublingual gland (SLG) are two of three major salivary glands in mammals and comprise serous and mucous acinar cells. The two glands share some functional properties, which are largely dependent on the types of acinar cells. In recent years, while ScRNA-seq (single-cell sequencing) with a 10 × platform has been used to explore molecular markers in salivary glands,
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How Do We Establish a Cold Chain Preservation Process Leading to Warmed Healthy Cells? Stem Cells Dev. (IF 4.0) Pub Date : 2023-11-16 Steve Oh
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Endothelial Cell Replacement of Human Veins, Modeling Vascular Repair and Endothelial Cell Chimerism. Stem Cells Dev. (IF 4.0) Pub Date : 2023-11-11 Hector Tejeda-Mora,Yvette den Hartog,Ivo Schurink,Monique Verstegen,Jeroen de Jonge,Martijn van den Hoogen,Carla C Baan,Robert Minnee,Martin J Hoogduijn,Luc J W van der Laan,Jorke Willemse
Allogeneic transplant organs are potentially highly immunogeneic. The endothelial cells (EC) located within the vascular system serve as the primary interface between the recipient's immune system and the donor organ, playing a key role in the allo-immune response. In this study, we investigated the potential use of recipient-derived ECs in a vein recellularization model. Here, human iliac veins underwent
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Defined Conditions Control the Morphological Dualism of Rat Primitive Extraembryonic Endoderm Stem Cells. Stem Cells Dev. (IF 4.0) Pub Date : 2023-11-10 Xiaoqiong Wang,Minjae Kim,Kyoung Hwa Jung,Young Gyu Chai,Bert Binas
Rat primitive extraembryonic endoderm (pXEN) stem cell lines indefinitely preserve the characteristic features of the early extraembryonic endoderm (ExEn) in vitro, but require unknown serum factors and exhibit a hybrid (mesenchymal-epithelial) phenotype. We report two chemically defined conditions that differ by the addition of the cytokine leukemia inhibitory factor (Lif) and the β-catenin-stabilizing
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R-Loop Defines Neural Stem/Progenitor Cells During Mouse Neurodevelopment. Stem Cells Dev. (IF 4.0) Pub Date : 2023-11-03 Zhe Zhang,Hanyue Zhang,Baoqi Hu,Yan Luan,Kun Zhu,Bo Ma,Zhichao Zhang,Xiaoyan Zheng
Neural stem/progenitor cells (NSPCs) are present in the mammalian brain throughout life and are involved in neurodevelopment and central nervous system repair. Although typical epigenetic signatures, including DNA methylation, histone modifications, and microRNAs, play a pivotal role in regulation of NSPCs, several of the epigenetic regulatory mechanisms of NSPCs remain unclear. Thus, defining a novel
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Development of Reproducible and Scalable Culture Conditions for In Vitro Maintenance of Pig Embryonic Stem Cells Using the Sandoz Inbred Swiss Mouse Thioguanine-Resistant Ouabain-Resistant Cell Line as a Feeder Layer. Stem Cells Dev. (IF 4.0) Pub Date : 2023-10-23 Yelim Ahn,Jinsol Jeong,Kwang-Hwan Choi,Dong-Kyung Lee,Mingyun Lee,Na-Young Lee,Dae-Yong Kim,Chang-Kyu Lee
Feeder cells play a crucial role in maintaining the pluripotency of embryonic stem cells (ESCs) by secreting various extrinsic regulators, such as extracellular matrix (ECM) proteins and growth factors. Although primary mouse embryonic fibroblasts (MEFs) are the most widely used feeder cell type for the culture of ESCs, they have inevitable disadvantages such as batch-to-batch variation and labor-intensive
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Retinal Organoid Models Show Heterozygous Rhodopsin Mutation Favors Endoplasmic Reticulum Stress-Induced Apoptosis in Rods. Stem Cells Dev. (IF 4.0) Pub Date : 2023-10-20 Qiaohui Yang,Jialin Li,Sicong Zeng,Zhuo Li,Xiao Liu,Jin Li,Wang Zhou,Yujiao Chai,Di Zhou
Retinitis pigmentosa (RP) is a prevalent inherited retinal degenerative disease resulting from photoreceptor and pigment epithelial apoptosis. The Rhodopsin (RHO) is the most commonly associated pathogenic gene in RP. However, RHO mutations (c.512C>T P171L) have been infrequently reported, and the RP pathogenesis caused by these mutations remains unclear. The objective of this study was to investigate
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The role of mesenchymal stem cells in hair regeneration and hair cycle. Stem Cells Dev. (IF 4.0) Pub Date : 2023-10-17 Cong Ma,Ming Cheng,Yan Wu,Xuegang Xu
The health of hair is directly related to people's health and appearance. Hair has key physiological functions, including skin protection and temperature regulation. Hair follicle is a vital mini-organ that directly impacts hair growth. Besides, various signaling pathways and molecules regulate the growth cycle transition of hair follicles. Hair and its regeneration studies have attracted much interest
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Dual Strategy with Adipose-derived Stem Cells and L-arginine Recovered Cavernosal Functions in a Rat Model of Radical Prostatectomy. Stem Cells Dev. (IF 4.0) Pub Date : 2023-10-17 Didem Yilmaz-Oral,Sena F Sezen,Damla Turkcan,Heba Asker,Ecem Kaya-Sezginer,Omer Faruk Kirlangic,Cagla Zubeyde Kopru,Mualla Pınar Elci,Fatma Zeynep Ozen,Petek Korkusuz,Sema Oren,Cetin Volkan Oztekin,Ilker Ates,Serap Gur
As standard therapy for prostate cancer, radical prostatectomy (RP) causes cavernous nerve injury and increases fibrosis and hypoxia-induced penile structural alterations. This study aimed to determine the potential beneficial effects of adipose-derived stem cells (ADSCs) and L-arginine alone or in combination on the penile erection of a rat model of erectile dysfunction (ED) caused by bilateral cavernous
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Mesenchymal Stromal Cells Regulate M1/M2 Macrophage Polarization in Mice with Immune Thrombocytopenia. Stem Cells Dev. (IF 4.0) Pub Date : 2023-09-29 Ziyang Liang,Guoyang Zhang,GuangTing Gan,Xiaoyan Liu,Hongyun Liu,Danian Nie,Liping Ma
Mesenchymal stromal cells have shown promising effects in the treatment of immune thrombocytopenia. However, the underlying mechanisms are not fully understood. In this study, we investigated the therapeutic effects of human bone marrow mesenchymal stromal cells (hBMSCs) and analyzed their unique role in regulating the M1/M2 macrophage ratio. We established a passive immune thrombocytopenia (ITP) mouse
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MicroRNAs as Prognostic Markers for Chondrogenic Differentiation Potential of Equine Mesenchymal Stromal Cells. Stem Cells Dev. (IF 4.0) Pub Date : 2023-09-27 A Hamed Alizadeh,Starlee Lively,Sarah Lepage,Pratibha Potla,Stewart Russell,Shabana Amanda Ali,Mohit Kapoor,Thomas G Koch
Mesenchymal stromal cells (MSCs) are a promising cell source for cartilage tissue regeneration in animals and humans but with large interdonor variation in their in vitro chondrogenic differentiation potential. Underlying molecular mechanisms responsible for culture-expanded MSC heterogeneity remain poorly understood. In this study, we sought to identify variations in microRNA (miRNA) signatures associated
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The Induction of Parathyroid Cell Differentiation from Human Induced Pluripotent Stem Cells Promoted Via TGF-α/EGFR Signaling. Stem Cells Dev. (IF 4.0) Pub Date : 2023-09-25 Ryusuke Nakatsuka,Tadashi Kato,Rong Zhang,Yasushi Uemura,Yuka Sasaki,Yoshikazu Matsuoka,Yasumasa Shirouzu,Tatsuya Fujioka,Hiromi Yamashita,Fumiyuki Hattori,Tadashige Nozaki,Hiroaki Ogata,Hirofumi Hitomi
The parathyroid gland plays an essential role in mineral and bone metabolism. Cultivation of physiological human parathyroid cells has yet to be established and the method by which parathyroid cells differentiate from pluripotent stem cells remains uncertain. Therefore, it has been hard to clarify the mechanisms underlying the onset of parathyroid disorders, such as hyperparathyroidism. In this study
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Senescent Stem Cell Dysfunction and Age-Related Diseases. Stem Cells Dev. (IF 4.0) Pub Date : 2023-09-15 Tongpeng Yue,Deguan Li
As the body ages, it experiences a gradual decline in the functioning of cells, tissues, and systems, which eventually leads to dysfunction and increased susceptibility to disease. At the cellular level, a reduction in the activity or number of stem cells is an important feature of cell senescence, and such changes may also directly drive the aging of the organism. Thus, finding ways to prevent or
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Fate Specification of GFAP-Negative Primitive Neural Stem Cells and Their Progeny at Clonal Resolution. Stem Cells Dev. (IF 4.0) Pub Date : 2023-09-11 Samantha Z Yammine,Ian Burns,Jessica Gosio,Andrew Peluso,Daniel M Merritt,Brendan Innes,Brenda L K Coles,Wen Rui Yan,Gary D Bader,Cindi M Morshead,Derek van der Kooy
The mature brain contains an incredible number and diversity of cells that are produced and maintained by heterogeneous pools of neural stem cells (NSCs). Two distinct types of NSCs exist in the developing and adult mouse brain: Glial Fibrillary Acidic Protein (GFAP)-negative primitive (p)NSCs and downstream GFAP-positive definitive (d)NSCs. To better understand the embryonic functions of NSCs, we
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Extracellular Matrix Influences Gene Expression and Differentiation of Mouse Trophoblast Stem Cells. Stem Cells Dev. (IF 4.0) Pub Date : 2023-08-14 Bryony V Natale,Ramie Kotadia,Katarina Gustin,Anirudha Harihara,Sarah Min,Michael J Kreisman,Kellie M Breen,David R C Natale
Trophoblast stem (TS) cells were first isolated from the mouse placenta; however, little is known about their maintenance and niche in vivo. TS cells, like other stem cells, have a unique microenvironment in which the extracellular matrix (ECM) is a component. Placental pathology is associated with ECM change. However, how these changes and the individual ECM components impact the maintenance or differentiation
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Impairment of HIF-2α Expression Induced the Compensatory Overexpression of the HIF-1α/SDF-1 Axis to Promote Wound Healing. Stem Cells Dev. (IF 4.0) Pub Date : 2023-08-11 Toshiharu Yamashita,Cat-Khanh Vuong,Nhat-Hoang Ngo,Motoo Osaka,Yuji Hiramatsu,Osamu Ohneda
Glucocorticoids are common anti-inflammatory factors; however, they have been reported to have side effects that delay the wound healing process. In a previous study, we found that mesenchymal stem cells isolated from the adipose tissue of patients with long-term glucocorticoid treatment (sAT-MSC) showed impaired wound healing ability due to the downregulation of SDF-1. In this study, we aimed to clarify
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Morphometric, Developmental, and Anti-Inflammatory Effects of Transamniotic Stem Cell Therapy (TRASCET) on the Fetal Heart and Lungs in a Model of Intrauterine Growth Restriction. Stem Cells Dev. (IF 4.0) Pub Date : 2023-08-01 Ashlyn E Whitlock,Kamila Moskowitzova,Ina Kycia,David Zurakowski,Dario O Fauza
Transamniotic stem cell therapy (TRASCET) with mesenchymal stem cells (MSCs) can attenuate placental inflammation and minimize intrauterine growth restriction (IUGR). We sought to determine whether MSC-based TRASCET could mitigate fetal cardiopulmonary effects of IUGR. Pregnant Sprague-Dawley dams were exposed to alternating 12-h hypoxia (10.5% O2) cycles in the last fourth of gestation. Their fetuses
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Etv5a Suppresses Neural Progenitor Cell Proliferation by Inhibiting sox2 Transcription. Stem Cells Dev. (IF 4.0) Pub Date : 2023-07-28 Hung-Yu Shih,Hao-Yuan Chen,Yin-Cheng Huang,Tu-Hsueh Yeh,Yi-Chieh Chen,Yi-Chuan Cheng
Neural progenitor cells are self-renewable, proliferative, and multipotent cell populations that generate diverse types of neurons and glia to build the nervous system. Transcription factors play critical roles in regulating various cellular processes; however, the transcription factors that regulate the development of neural progenitors are yet to be identified. In the present study, we demonstrated
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Human-Induced Hepatocytes-Derived Extracellular Vesicles Ameliorated Liver Fibrosis in Mice Via Suppression of TGF-β1/Smad Signaling and Activation of Nrf2/HO-1 Signaling. Stem Cells Dev. (IF 4.0) Pub Date : 2023-07-24 Wenjing Liu,Jiajun Wu,Huiying Cao,Chen Ma,Zhitao Wu,Youxi Tian,Chenhui Ma,Hong Qiu,Guoyu Pan
Liver fibrosis is a wound-healing response caused by persistent liver injury and often occurs in chronic liver diseases. Effective treatments for liver fibrosis are still pending. Recent studies have revealed that extracellular vesicles (EVs) derived from primary hepatocytes (Hep-EVs) have therapeutic potential for multiple liver diseases. However, Hep-EVs are difficult to manufacture in bulk because
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Blastocyst Cell Number and Allocation Affect the Developmental Potential and Transcriptome of Bovine Somatic Cell Nuclear Transfer Embryos. Stem Cells Dev. (IF 4.0) Pub Date : 2023-07-18 Pablo J Ross,Marcelo D Goissis,João P N Martins,James L Chitwood,J Richard Pursley,Guilherme J M Rosa,Jose B Cibelli
Cloning cattle using somatic cell nuclear transfer (SCNT) is inefficient. Although the rate of development of SCNT embryos in vitro is similar to that of fertilized embryos, most fail to develop into healthy calves. In this study, we aimed to identify developmentally competent embryos according to blastocyst cell composition and perform transcriptome analysis of single embryos. Transgenic SCNT embryos
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Neurite Outgrowth and Gene Expression Profile Correlate with Efficacy of Human Induced Pluripotent Stem Cell-Derived Dopamine Neuron Grafts. Stem Cells Dev. (IF 4.0) Pub Date : 2023-06-22 Rachel Hills,Jim A Mossman,Andres M Bratt-Leal,Ha Tran,Roy M Williams,David G Stouffer,Irina V Sokolova,Pietro P Sanna,Jeanne F Loring,Mariah J Lelos
Transplantation of human induced pluripotent stem cell-derived dopaminergic (iPSC-DA) neurons is a promising therapeutic strategy for Parkinson's disease (PD). To assess optimal cell characteristics and reproducibility, we evaluated the efficacy of iPSC-DA neuron precursors from two individuals with sporadic PD by transplantation into a hemiparkinsonian rat model after differentiation for either 18
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Parallel Genome-Wide CRISPR Screens to Identify State-Dependent Self-Renewal Regulators of Mouse Embryonic Stem Cells. Stem Cells Dev. (IF 4.0) Pub Date : 2023-06-20 Jiangjun Wang,Chen Zhang,Yi Huang,Yan Ruan,Yan Hu,Jiaqi Wang,Fengsheng Wang,Meng Yu,Yixiao Xu,Lianlian Liu,Yuda Cheng,Ran Yang,Yutong Dong,Jiali Wang,Yi Yang,Jiaxiang Xiong,Yanping Tian,Qiangguo Gao,Junlei Zhang,Rui Jian
The pluripotency of embryonic stem cells (ESCs) is more accurately viewed as a continuous developmental process rather than a fixed state. However, the factors that play general or state-specific roles in regulating self-renewal in different pluripotency states remain poorly defined. In this study, parallel genome-wide CRISPR/Cas9 knockout (KO) screens were applied in ESCs cultured in the serum plus
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Effect of Insulin on Bone Formation Ability of Rat Alveolar Bone Marrow Mesenchymal Stem Cells. Stem Cells Dev. (IF 4.0) Pub Date : 2023-07-14 Lingling E,Rongjian Lu,Ying Zheng,Li Zhang,Xiaocao Ma,Yan Lv,Mingzhu Gao,Shaoli Zhang,Limei Wang,Hongchen Liu,Rong Zhang
The alveolar bone marrow mesenchymal stem cells (ABM-MSCs) play an important role in oral bone healing and regeneration. Insulin is considered to improve impaired oral bones due to local factors, systemic factors and pathological conditions. However, the effect of insulin on bone formation ability of ABM-MSCs still needs to be elucidated. The aim of this study was to determine the responsiveness of
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Apelin-Overexpressing Neural Stem Cells in Conjunction with a Silk Fibroin Nanofiber Scaffold for the Treatment of Traumatic Brain Injury. Stem Cells Dev. (IF 4.0) Pub Date : 2023-08-16 Tianwen Li,Qisheng Tang,Jiaxin Xu,Xiangru Ye,Kezhu Chen,Junjie Zhong,Jianhong Zhu,Shijun Lu,Tongming Zhu
Traumatic brain injury (TBI), especially moderate or severe TBI, is one of the most devastating injuries to the nervous system, as the existing therapies for neurological defect repair have difficulty achieving satisfactory results. Neural stem cells (NSCs) therapy is a potentially effective treatment option, especially after specific genetic modifications and when used in combination with biomimetic
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Small Extracellular Vesicles Maintain Homeostasis of Senescent Mesenchymal Stem Cells at Least Through Excreting Harmful Lipids. Stem Cells Dev. (IF 4.0) Pub Date : 2023-08-04 Liping Wang,Huan Zhang,Xian Xiao,Shihua Wang,Robert Chunhua Zhao
Mesenchymal stem cells (MSCs) play an essential role in multiple physiological processes in vivo and a promising cell-based therapy for various diseases. Nonetheless, MSCs suffer from senescence with expansion culture, leading to a limitation for their clinical application. Recently, it was reported that small extracellular vesicles (sEVs) are involved in regulation of senescence in tumor cells and
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Identification and Functional Evaluation of Alternative Splice Variants of Dax1 in Mouse Embryonic Stem Cells. Stem Cells Dev. (IF 4.0) Pub Date : 2023-07-14 Jiaqi Wang,Yi Huang,Chen Zhang,Yan Ruan,Yanping Tian,Fengsheng Wang,Yixiao Xu,Meng Yu,Jiangjun Wang,Yuda Cheng,Lianlian Liu,Ran Yang,Jiali Wang,Yi Yang,Jiaxiang Xiong,Yan Hu,Rui Jian,Bing Ni,Wei Wu,Junlei Zhang
Dax1 (Nr0b1; Dosage-sensitive sex reversal-adrenal hypoplasia congenital on the X-chromosome gene-1) is an important component of the transcription factor network that governs pluripotency in mouse embryonic stem cells (ESCs). Functional evaluation of alternative splice variants of pluripotent transcription factors has shed additional insight on the maintenance of ESC pluripotency and self-renewal
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The Role of Macrophage Phenotype in the Vascularization of Prevascularized Human Bone Marrow Mesenchymal Stem Cell Sheets. Stem Cells Dev. (IF 4.0) Pub Date : 2023-06-01 Rui Chen,Siqi Long,Lina Ren,Sen Xu,Xiaoning Liu,Jiamin Shi,Jiaxin Liu,Dongyang Ma,Ping Zhou,Liling Ren
With the development of tissue engineering and regenerative medicine, prevascularized bone marrow mesenchymal stem cell (BMSC) sheets have been regarded as a promising method for tissue regeneration. Furthermore, the inflammatory response is one of the main regulators of vascularization and the restoration of engineered tissue function; among them, macrophages and cytokines produced by them are considered
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Deletion of p66Shc Dysregulates ERK and STAT3 Activity in Mouse Embryonic Stem Cells, Enhancing Their Naive-Like Self-Renewal in the Presence of Leukemia Inhibitory Factor. Stem Cells Dev. (IF 4.0) Pub Date : 2023-06-13 Andrew M Powell,Nicole A Edwards,Hailey Hunter,Patti Kiser,Andrew J Watson,Robert C Cumming,Dean H Betts
The ShcA adapter protein is necessary for early embryonic development. The role of ShcA in development is primarily attributed to its 52 and 46 kDa isoforms that transduce receptor tyrosine kinase signaling through the extracellular signal regulated kinase (ERK). During embryogenesis, ERK acts as the primary signaling effector, driving fate acquisition and germ layer specification. P66Shc, the largest
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FoxO1 Agonists Promote Bone Regeneration in Periodontitis by Protecting the Osteogenesis of Periodontal Ligament Stem Cells. Stem Cells Dev. (IF 4.0) Pub Date : 2023-06-06 Xiaojun Huang,Xiaoxia Su,Qizhao Ma,Yongting Xie,Qiang Guo,Li Liao,Jing Zou
Protecting the function of periodontal ligament stem cells (PDLSCs) is crucial for bone regeneration in periodontitis. Forkhead box protein O1 (FoxO1) has been previously reported as a crucial mediator in bone homeostasis, providing a favorable environment for osteoblast proliferation and differentiation. In this study, we investigated the effect and mechanism of FoxO1 agonists on the osteogenesis
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Pax6-Induced Proliferation and Differentiation of Bone Marrow Mesenchymal Stem Cells Into Limbal Epithelial Stem Cells. Stem Cells Dev. (IF 4.0) Pub Date : 2023-05-31 Jie Gao,Ling Ding,Ying Xin,Yuandi Li,Keke He,Min Su,Rong Hu
Corneal integrity, transparency, and visual acuity are maintained by corneal epithelial cells (CECs), which are continuously renewed by limbal epithelial stem cells (LESCs). The limbal stem cell deficiency is associated with ocular diseases. This study aimed to develop a novel method to differentiate bone marrow mesenchymal stem cells (BM-MSCs) into LESC-like cells using a culture medium and paired
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Single-Cell Sequencing Informs That Mesenchymal Stem Cell Alleviates Renal Injury Through Regulating Kidney Regional Immunity in Lupus Nephritis. Stem Cells Dev. (IF 4.0) Pub Date : 2023-05-17 Cheng Zhou,Xinlong Bai,Yunzhao Yang,Meihan Shi,Xue-Yuan Bai
Lupus nephritis (LN) is the common complication of systemic lupus erythematosus. The pathogenesis of LN kidney injury is unclear. In addition to systemic (extrarenal) immune cells, local (intrarenal) immune cells residing in "kidney regional immunity" are momentous in LN. Mesenchymal stem cell (MSC) therapy is effective for LN. However, mechanisms of MSC therapy remains unclear. In this study, we first
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KDM6A-Mediated Regulation of Cranial Frontal Bone Suture Fusion in Mice Is Sex Dependent. Stem Cells Dev. (IF 4.0) Pub Date : 2023-05-22 Clara Pribadi,Dimitrios Cakouros,Esther Camp,Peter Anderson,Stan Gronthos
The five flat bones of developing cranial plates are bounded by fibrous sutures, which remain open during development to accommodate for the growing brain. Kdm6A is a demethylase that removes the epigenetic repressive mark, trimethylated lysine 27 on histone 3 (H3K27me3), from the promoters of osteogenic genes, and has previously been reported to promote osteogenesis in cranial bone cells. This study
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In Vitro Transdifferentiation Potential of Equine Mesenchymal Stem Cells into Schwann-Like Cells. Stem Cells Dev. (IF 4.0) Pub Date : 2023-06-07 Lucas Vinícius de Oliveira Ferreira,Beatriz da Costa Kamura,João Pedro Marmol de Oliveira,Natielly Dias Chimenes,Márcio de Carvalho,Leandro Alves Dos Santos,Luciane Alarcão Dias-Melicio,Renée Laufer Amorim,Rogério Martins Amorim
Schwann cells (SCs) are essential for the regenerative processes of peripheral nerve injuries. However, their use in cell therapy is limited. In this context, several studies have demonstrated the ability of mesenchymal stem cells (MSCs) to transdifferentiate into Schwann-like cells (SLCs) using chemical protocols or co-culture with SCs. Here, we describe for the first time the in vitro transdifferentiation
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Genetically Modified Mesenchymal Stromal Cells in Cartilage Regeneration. Stem Cells Dev. (IF 4.0) Pub Date : 2023-05-11 Yujun Sun,Chunyu Xue,Haoyu Wu,Changchuan Li,Shixun Li,Jiankai Luo,Taihe Liu,Yue Ding
Articular cartilage injury is common in various conditions, including osteoarthritis, rheumatic diseases, and trauma. Current treatments for cartilage injury fail to completely regenerate the damaged cartilage. Mesenchymal stromal cells (MSCs) have emerged as potential candidates for cartilage regeneration. However, MSCs exhibit hypertrophic differentiation, and their chondrogenic ability is reduced
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Progress of Bone Marrow Mesenchymal Stem Cell Mitochondrial Transfer in Organ Injury Repair. Stem Cells Dev. (IF 4.0) Pub Date : 2023-05-02 Xuezhou Yang,Kaiting Ning,Dong-En Wang,Huiyun Xu
There has been an upsurge of interest in the bone marrow mesenchymal stem cell (BMSC) mitochondrial transfer as a potential therapeutic innovation in organ injury repair. Previous research mainly focused on its transfer routes and therapeutic effects. However, its intrinsic mechanism has not been well deciphered. The current research status needs to be summarized for the clarification of future research
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Human Neural Stem Cell Secretome Inhibits Neuron Heme Uptake and Ferroptosis in Intracerebral Hemorrhage Through Nrf-2 Signaling Pathway. Stem Cells Dev. (IF 4.0) Pub Date : 2023-04-24 Xiaorui Lv,Yating Ling,Dongdong Niu,Yu Zeng,Yun Qiu,Yu Si,Tao Guo,Yinying Ni,Jingwen Zhang,Ziyu Wang,Jiabo Hu
Intracerebral hemorrhage (ICH) is a common subtype of stroke with a very high mortality rate, but there is still no effective cure. Increasing evidence suggests that heme accumulation and neuronal ferroptosis play an important role in secondary injury after ICH. Neural stem cells (NSCs), as seed cells of the central nervous system, have received much attention due to their abundant paracrine product
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Reactive Oxygen Species-Induced Inhibition of Odontoblastic Differentiation of Mouse Dental Papilla Cells Is Mediated by Downregulation of Importin 7. Stem Cells Dev. (IF 4.0) Pub Date : 2023-03-17 Ziqiu Xiao,Yue Zhang,Guohua Yuan,Guobin Yang
Tooth dentin is a crucial tooth structure. The biological process of odontoblast differentiation is essential for formation of normal dentin. Accumulation of reactive oxygen species (ROS) leads to oxidative stress, which can influence the differentiation of several cells. As a member of the importin-β superfamily, importin 7 (IPO7) is essential for nucleocytoplasmic transport and plays an important
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Milk Fat Globule Epidermal Growth Factor 8a Regulates Neurogenesis in Telencephalon and Affects Larval Behavior in Zebrafish. Stem Cells Dev. (IF 4.0) Pub Date : 2023-03-17 Yongfeng Luo,Dalu Lu,Jiefei Zhou,Jiang Wu,Weiye Wang,Linxi Qian
Mfge8, a secreted glycoprotein, is a key molecule that mediates the phagocytosis of apoptotic cells. Previous research reported that Mfge8 is critical for the proliferation and differentiation of radial glial cells (RGCs) in the dentate gyrus of adult mice. The treatment of Mfge8 is also beneficial for the repair of central nervous system (CNS) injury after cerebral ischemia. This study aimed to investigate
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Notch Pathways Regulate Expression of Angiostatic Factor Activin A in Endothelial-Pericyte-Like Mesenchymal Stromal Cell Interactions. Stem Cells Dev. (IF 4.0) Pub Date : 2023-05-16 Stephanie Merfeld-Clauss,Hongyan Lu,Keith L March,Dmitry O Traktuev
Vascularization of ischemic and fabricated tissues is essential for successful tissue repair and replacement therapies. Endothelial cells (ECs) and mesenchymal stem/stromal cells (MSCs) in close proximity spontaneously organize into vessels after coimplantation in semisolid matrices. Thus, local injection of EC mixed with MSC may facilitate tissue (re)vascularization. The organization of these cells
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Intravenous Injection of Equine Mesenchymal Stem Cells in Dogs with Articular Pain and Lameness: A Feasibility Study. Stem Cells Dev. (IF 4.0) Pub Date : 2023-04-19 Carlien Brondeel,Frederik Weekers,Lore van Hecke,Eva Depuydt,Glenn Pauwelyn,Geert Verhoeven,Nathalie de Bouvré,Peter De Roeck,Peter Vandekerckhove,Pieter Vanacker,Davina Böhm-Geerdink,Robert Daems,Luc Duchateau,Jimmy Saunders,Yves Samoy,Jan H Spaas
Osteoarthritis is a frequently occurring joint disorder in veterinary practice. Current treatments are focused on pain and inflammation; however, these are not able to reverse the pathological condition. Mesenchymal stem cells (MSCs) could provide an interesting alternative because of their immunomodulatory properties. The objective of this study was to evaluate the potential of a single intravenous
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mTORC1-Induced Bone Marrow-Derived Mesenchymal Stem Cell Exhaustion Contributes to the Bone Abnormalities in Klotho-Deficient Mice of Premature Aging. Stem Cells Dev. (IF 4.0) Pub Date : 2023-04-17 Ran Feng,Su Wu,Ruofei Li,Kunling Huang,Ting Zeng,Zhifen Zhou,Xiaoqin Zhong,Zhou Songyang,Feng Liu
Stem cell exhaustion is a hallmark of aging. Klotho-deficient mice (kl/kl mice) is a murine model that mimics human aging with significant bone abnormalities. The aim of this study is using kl/kl mice to investigate the functional change of bone marrow-derived mesenchymal stem cells (BMSCs) and explore the underlying mechanism. We found that klotho deficiency leads to bone abnormalities. In addition
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Opening the "Black Box" Underlying Barriers to the Use of Canine Induced Pluripotent Stem Cells: A Narrative Review. Stem Cells Dev. (IF 4.0) Pub Date : 2023-04-17 Alexander G Kuzma-Hunt,Vipra Shah,Sierra DiMarco,Keith A Russell,Dean H Betts,Thomas G Koch
Induced pluripotent stem cells (iPSCs) are produced by resetting the epigenetic and transcriptional landscapes of somatic cells to express the endogenous pluripotency network and revert them back to an undifferentiated state. The reduced ethical concerns associated with iPSCs and their capacity for extensive self-renewal and differentiation make them an unparalleled resource for drug discovery, disease
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Nicotine Reduces Reactive Oxygen Species and Enhances Cell Proliferation via the α4 Nicotinic Acetylcholine Receptor Subunit in Human Induced Pluripotent Stem Cells. Stem Cells Dev. (IF 4.0) Pub Date : 2023-03-30 Youichi Ohno,Daisuke Taura,Kentaro Okamoto,Haruka Fujita,Kyoko Honda-Kohmo,Koji Matsuo,Masakatsu Sone
The effects of smoking on fetal development and stem cell differentiation are not fully understood. Although nicotinic acetylcholine receptors (nAChRs) are expressed in many organs of the human body, their significance in human induced pluripotent stem cells (hiPSCs) remains unclear. After expression levels of nAChR subunits in hiPSCs were determined, the effects of the nAChR agonist, nicotine, on
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Activation of the Interferon Pathway in Trophoblast Cells Productively Infected with SARS-CoV-2. Stem Cells Dev. (IF 4.0) Pub Date : 2023-03-22 Sampada Kallol,Laura Martin-Sancho,Robert Morey,Omonigho Aisagbonhi,Donald Pizzo,Morgan Meads,Sumit K Chanda,Francesca Soncin
SARS-CoV-2 infection during pregnancy has been associated with poor maternal and neonatal outcomes and placental defects. The placenta, which acts as a physical and immunological barrier at the maternal-fetal interface, is not established until the end of the first trimester. Therefore, localized viral infection of the trophoblast compartment early in gestation could trigger an inflammatory response
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Human Adipose-Derived Mesenchymal Stem Cell-Based Microspheres Ameliorate Atherosclerosis Progression In Vitro. Stem Cells Dev. (IF 4.0) Pub Date : 2023-04-03 Shaojie Yang,Xiong Xiao,Ziwei Huang,Qingyun Chen,Chenxi Li,Chuan Niu,Yuchu Yang,Liping Yang,Bing-Mei Zhu,Li Feng
Atherosclerosis (AS) is a chronic inflammatory disease associated with lipid deposition, which could be converted into acute clinical events by thrombosis or plaque rupture. Adipose-derived mesenchymal stem cell (ADSC)-encapsulated repair units could be an effective cure for the treatment of AS patients. In this study, we encapsulate human adipose-derived mesenchymal stem cells (hADSCs) in collagen
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Characterization of Oogonial Stem Cells in Adult Mouse Ovaries with Age and Comparison to In Silico Data on Human Ovarian Aging Stem Cells Dev. (IF 4.0) Pub Date : 2023-02-03 Julie A. MacDonald, Hannah C. Sheehan, Andrew Piasecki, Luciana R. Faustino, Charlotte Hauschildt, Victor Stolzenbach, Dori C. Woods, Jonathan L. Tilly
Many adult somatic stem cell lineages are comprised of subpopulations that differ in gene expression, mitotic activity, and differentiation status. In this study, we explored if cellular heterogeneity also exists within oogonial stem cells (OSCs), and how chronological aging impacts OSCs. In OSCs isolated from mouse ovaries by flow cytometry and established in culture, we identified subpopulations
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Safety and efficacy of allogeneic umbilical cord blood therapy for global development delay and intellectual disability Stem Cells Dev. (IF 4.0) Pub Date : 2023-02-03 Byoungwoo Cha, Hyunseok Kwak, Ji-In Bang, Prof. Su Jin Jang, Mi Ri Suh, Jee In Choi, Prof. MinYoung Kim
Most pediatric patients with global developmental delay (GDD) or intellectual disability (ID) have disrupted development. Since allogeneic umbilical cord blood (UCB) may exert neurotrophic effects, a prospective clinical trial was conducted to assess the efficacy and safety of UCB therapy for GDD and ID. A total of thirteen children (aged 6 months to 15 years) with GDD and ID were enrolled and followed-up
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Cellular therapies: yesterday, today, and tomorrow Stem Cells Dev. (IF 4.0) Pub Date : 2023-02-02 Dr. Héctor Mayani
Cellular therapy (CT) can be defined as the transference into a person of healthy cells in order to correct defective functions. Yesterday (1950 – 2010), CT consisted mostly of hematopoietic transplants for the treatment of a variety of hematological disorders. Interestingly, during that period of time other cell types with therapeutic potential -including certain lymphoid populations and other non-hematopoietic
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Transamniotic Fetal Administration of Genetically Modified Hematopoietic Stem Cells Carrying a Human Transgene in a Syngeneic Rat Model Stem Cells Dev. (IF 4.0) Pub Date : 2023-01-31 Dr. Daniel Labuz, Dr. Ashlyn Whitlock, Ina Kycia, Dr. David Zurakowski, Dr. Dario O. Fauza
Hematopoietic stem cell (HSC)-based gene therapy has already reached clinical reality in a few applications. Fetal administration of genetically modified HSCs has only been feasible to date via invasive and morbid methods. It has been recently shown that native donor HSCs can reach the fetal circulation and bone marrow after simple delivery into the amniotic fluid, at least in a syngeneic healthy model
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Deciphering Adult Neural Stem Cells with Single-cell Sequencing Stem Cells Dev. (IF 4.0) Pub Date : 2023-01-31 Dr. Yidan Liu, Yingting Zhu, Yunhong Shi, Xiuxing Liu, Dr. Wenru Su, Yehong Zhuo
Adult neural stem cells (NSCs) are restricted to the two neurogenic regions of the mammalian brain, where they self-renew and generate progenies of multiple lineages, including neurons, astrocytes, and oligodendrocytes. Single-cell RNA sequencing technology, which reconstructs high-resolution transcriptional landscapes, provides valuable insights into cellular heterogeneity and developmental dynamics
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Expression of Stem Cell Niche-Related Biomarkers at the Base of the Human Tricuspid Valve Stem Cells Dev. (IF 4.0) Pub Date : 2023-01-27 Jacob Sjölin, Marianne Jonsson, Charlotta Orback, Anders Oldfors, Anders Jeppsson, Jane Synnergren, Victoria Rotter Sopasakis, Kristina Vukusic
Stem cell niches have been thoroughly investigated in tissue with high regenerative capacity but not in tissues where cell turnover is slow, such as the human heart. The left AtrioVentricular junction (AVj), the base of the mitral valve, has previously been proposed as a niche region for cardiac progenitors in the adult human heart. In the present study, we explore the right side of the human heart
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cAMP Agonist Forskolin Disrupts Mitochondrial Metabolism and Induces Senescence in Human Mesenchymal Cells Stem Cells Dev. (IF 4.0) Pub Date : 2023-01-25 Qiaoling Wang, Xiaodong Su, Rongjia Zhu, Robert Chunhua Zhao
Adult-derived mesenchymal stem cells (MSCs) can be used in therapies for the treatment of various diseases. The MSCs derived from aging tissues or long-term MSC cultures could have diminished therapeutic effects compared with MSCs derived from younger tissues, but the underlying mechanism has not been completely established. Dysfunction of energy metabolism is one of the main mechanisms underlying
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Alteration of Transcriptomic Profile and Antiseptic Efficacy of Adipose-Derived Mesenchymal Stromal/Stem Cells Under Different Culture Conditions Stem Cells Dev. (IF 4.0) Pub Date : 2023-01-25 Chen Chen, Xiaoming Hou, Fujia Jing, Tao Wang, Li Feng, Y. James Kang
Mesenchymal stromal/stem cells (MSCs) are a promising therapeutic agent for various diseases, including sepsis. However, translating MSC therapy to clinical applications remains challenging due to variations in the properties of MSCs under different preparation conditions. In this study, the gene expression profiles of human adipose-derived mesenchymal stromal/stem cells (ADSCs) under different culture
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Acetylcholine Receptors in Mesenchymal Stem Cells Stem Cells Dev. (IF 4.0) Pub Date : 2023-01-24 Saeed Alqahtani, Mark C. Butcher, Gordon Ramage, Matthew J. Dalby, William McLean, Christopher J. Nile
Mesenchymal stem cells (MSCs) are well known for their regenerative potential. Even though the ability of MSCs to proliferate and differentiate has been studied extensively, there remains much to learn about the signaling mechanisms and pathways that control proliferation and influence the differentiation phenotype. In recent years, there has been growing evidence for the utility of non-neuronal cholinergic
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Bone marrow mesenchymal stem cell-derived exosomes alleviate peritoneal dialysis-associated peritoneal injury Stem Cells Dev. (IF 4.0) Pub Date : 2023-01-24 Ms. Fang Yu, Jie Yang, Ms. Jia Chen, Xiaoyue Wang, Qingli Cai, Yani He, Dr. Kehong Chen
Peritoneal fibrosis is a critical sequela that limits the application of peritoneal dialysis (PD). This study explored the role and mechanism of bone marrow mesenchymal stem cell-derived exosomes (BMSC-Exos) in preventing PD-associated peritoneal injury. C57BL/6 mice were randomized into three groups: a control (saline), peritoneal injury (2.5% glucose peritoneal dialysate + lipopolysaccharide (LPS))