样式: 排序: IF: - GO 导出 标记为已读
-
Recent Advances in Mesenchymal Stem/Stromal Cell-Based Therapy for Alcohol-Associated Liver Disease and Non-alcoholic Fatty Liver Disease. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-11-28 Foteini Korkida,Alexandra Stamatopoulou,Maria G Roubelakis
Alcohol-associated liver disease (ALD) and non-alcoholic fatty liver disease (NAFLD) represent pathological conditions that include many distinct stages, potentially leading to the final stage of cirrhotic liver. To date, liver transplantation is the sole successful treatment with concomitant limitations related to donor organ shortage and the need of life-long immunosuppressive therapy. Recently,
-
Probing Multiple Transplant Delivery Routes of CD+34 Stem Cells for Promoting Behavioral and Histological Benefits in Experimental Ischemic Stroke. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-11-28 Jea-Young Lee,Justin Cho,Francesco D'Egidio,Christine Vignon,Hendrik Streefkerk,Matthieu de Kalbermatten,Ibon Garitaonandia,Cesar V Borlongan
Stroke is a leading cause of death in the US and around the world but with limited treatment options. Survivors often present with long-term cognitive and neurological deficits. Stem cell-based therapy has emerged as a potential treatment for stroke. While stem cell transplantation in stroke has reached clinical trials, mostly safety outcomes have been reported with efficacy readouts warranting more
-
-
Cell Therapy Improves Quality-of-Life in Heart Failure: Outcomes From a Phase III Clinical Trial. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-11-24 Satsuki Yamada,Jozef Bartunek,Thomas J Povsic,Gad Cotter,Beth A Davison,Christopher Edwards,Atta Behfar,Marco Metra,Gerasimos S Filippatos,Marc Vanderheyden,William Wijns,Andre Terzic
Patients with heart failure experience limitations in daily activity and poor quality-of-life. Prospective surveillance of health-related quality-of-life supplemented traditional death and hospitalization outcomes in the multinational, randomized, double-blinded CHART-1 clinical trial that assessed cardiopoiesis-guided cell therapy in ischemic heart failure patients with reduced left ventricular ejection
-
Cell-Based Therapy by Autologous Bone Marrow-Derived Mononuclear Cells for bone Augmentation of Plate-Stabilized Proximal Humeral Fractures - A Multicentric, Randomized, Open Phase IIa study. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-11-23 Caroline Seebach,Christoph Nau,Dirk Henrich,Rene Verboket,Marlene Bellen,Nadine Frischknecht,Vivien Moeck,Kathrin Eichler,Kay Hajo Schmidt Horlohé,Reinhard Hoffmann,Halvard Bonig,Erhard Seifried,Johannes Frank,Ingo Marzi
Proximal humerus fractures are common in an aging population. The standard operative treatment is open reduction internal fixation (ORIF) using an angular stable plate. However, this procedure has complications such as a relatively high rate of secondary dislocation, humeral head necrosis or nonunion caused by delayed bony consolidation. Autologous bone marrow mononuclear cells (BMC) combined with
-
Effect of Valproic Acid on Promoting the Differentiation of Human Embryonic Stem Cells Into Cholangiocyte-Like Cells. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-11-23 Shuai Deng,Xiaoyu Zhao,Ziyan Kou,Yanlun Zhu,Xuerao Zhang,Hon Fai Chan
Cholangiocytes form a complex 3D network of bile ducts in the liver and contribute to liver function. The damage or destruction of cholangiocytes can lead to biliary diseases, and the shortage of cholangiocytes remains an obstacle for drug development targeting biliary diseases. Valproic acid (VPA) is a potent activator of Notch signaling pathway that is essential for cholangiocyte differentiation
-
Extracellular Vesicles From Mesenchymal Umbilical Cord Cells Exert Protection Against Oxidative Stress and Fibrosis in a Rat Model of Bronchopulmonary Dysplasia. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-11-15 Paola Bisaccia,Fabio Magarotto,Stefania D'Agostino,Arben Dedja,Silvia Barbon,Diego Guidolin,Cristina Liboni,Roberta Angioni,Giada De Lazzari,Federico Caicci,Antonella Viola,Marcin Jurga,Gabrielis Kundrotas,Dimitri Stevens,Domenico Mancuso,Elisabetta Gramegna,Bruno Seitaj,Rudra Kashyap,Beatrice De Vos,Veronica Macchi,Eugenio Baraldi,Andrea Porzionato,Raffaele De Caro,Maurizio Muraca,Michela Pozzobon
Oxidative stress and fibrosis are important stress responses that characterize bronchopulmonary dysplasia (BPD), a disease for which only a therapy but not a cure has been developed. In this work, we investigated the effects of mesenchymal stromal cells-derived extracellular vesicles (MSC-EVs) on lung and brain compartment in an animal model of hyperoxia-induced BPD. Rat pups were intratracheally injected
-
Clinical Application of Umbilical Cord Mesenchymal Stem Cells Preserves β-cells in Type 1 Diabetes. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-11-09 Ashraf Al Madhoun,Lubaina Koti,Neus Carrió,Maher Atari,Fahd Al-Mulla
Type 1 diabetes (T1D) is a chronic autoimmune disease associated with complications that reduce the quality of life of affected individuals and their families. The therapeutic options for T1D are limited to insulin therapy and islet transplantation; these options are not focused on preserving β-cell function and endogenous insulin. Despite the promising outcomes observed in current clinical trials
-
Sfrp1 as a Pivotal Paracrine Factor in the Trained Pericardial Stem Cells that Foster Reparative Activity. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-11-04 Hongtao Zhu,Xueqing Liu,Weili Ouyang,Yingcai Hao,Zheheng Ding,Kezhe Tan,Jianfeng Tang,Jianfeng Zhao,Xiaojun Ding,Zenghui Teng,Xiaoming Deng,Weidong Wu,Zhaoping Ding
Tissue damage often induces local inflammation that in turn dictates a series of subsequential responses, such as stem cell activation and growth, to maintain tissue homeostasis. The aim of the study is to testify the possibility of using inflammation-trained stem cells as optimal donor cells to augment the efficacy of cell therapy. The pericardial stem/stromal cells derived from the animals after
-
Using A Google Web Search Analysis to Assess the Utility of ChatGPT in Stem Cell Therapy. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-11-04 Long Chen,Hui Li,Yiqi Su,Zhen Yang,Zihao He,Du Wang,Jiao Jiao Li,Dan Xing
OBJECTIVE Since its introduction, the use of ChatGPT has increased significantly for medically related purposes. However, current research has not captured its applications in providing information on stem cell therapy. To address this gap, the present study compared the effectiveness of ChatGPT to Google in answering medical questions related to stem cell therapy. METHODS The search term "stem cell
-
3D Spheroids Facilitate Differentiation of Human Adipose-Derived Mesenchymal Stem Cells into Hepatocyte-Like Cells via p300-Mediated H3K56 Acetylation. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-11-04 Yanrong Yu,Haina Huang,Junsong Ye,Yumei Li,Renjian Xie,Liping Zeng,Yushan Huang,Tai Zeng,Dan Luo,Jianing Zhong,Weijie Peng
Hepatocyte-like cells (HLCs) that are differentiated from mesenchymal stem cells (MSCs) provide a valuable resource for drug screening and cell-based regeneration therapy. Differentiating HLCs into 3D spheroids enhances their phenotypes and functions. However, the molecular mechanisms underlying MSCs hepatogenic differentiation are not fully understood. In this study, we generated HLCs from human adipose-derived
-
Long-Term Stability of Cord Blood Units After 29 Years of Cryopreservation: Follow-Up Data From the José Carreras Cord Blood Bank. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-11-04 Stefanie Liedtke,Sabine Többen,Holger Gressmann,Andrea Meyer,Pablo E Verde,Eliane Gluckman,Gesine Kogler
The José Carreras Cord Blood Bank (CBB) located in Düsseldorf as of today stores 21 215 active cryopreserved cord blood units (CBUs) applicable as a source for hematopoietic stem cell (HSC) transplantation. Since the success of transplantation outcomes is mainly dependent on the cord blood quality, typical parameters are evaluated by a Stability Monitoring Program specified by the FACT Standards. The
-
Sstr2 Defines the Cone Differentiation-Competent Late-Stage Retinal Progenitor Cells in the Developing Mouse Retina. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-11-03 Yihan Bai,Han He,Bangqi Ren,Jiayun Ren,Ting Zou,Xi Chen,Yong Liu
Cone cell death is a characteristic shared by various retinal degenerative disorders, such as cone-rod dystrophy, Stargardt disease, achromatopsia, and retinitis pigmentosa. This leads to conditions like color blindness and permanently impaired visual acuity. Stem cell therapy focused on photoreceptor replacement holds promise for addressing these conditions. However, identifying surface markers that
-
Preconditioning Concepts for the Therapeutic Use of Extracellular Vesicles Against Stroke. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-11-03 Matteo Haupt,Stefan T Gerner,Hagen B Huttner,Thorsten R Doeppner
Various preclinical stroke models have demonstrated the neuroprotective effects of extracellular vesicles (EVs) obtained from several types of cells, including neurons, astrocytes, microglia, neuronal progenitor cells, bone marrow stem cells, and mesenchymal stem cells. EVs interfere with key mechanisms in stroke pathophysiology such as cell death, neuroinflammation, autophagy, and angiogenesis. The
-
Human Neural Progenitors Expressing GDNF Enhance Retinal Protection in a Rodent Model of Retinal Degeneration. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-11-03 Saba Shahin,Patrick Tan,Jason Chetsawang,Bin Lu,Soshana Svendsen,Stephany Ramirez,Trevor Conniff,Jorge S Alfaro,Michael Fernandez,Aaron Fulton,Alexander H Laperle,Clive N Svendsen,Shaomei Wang
Stem cell therapy for retinal degenerative diseases has been extensively tested in preclinical and clinical studies. However, preclinical studies performed in animal models at the early stage of disease do not optimally translate to patients that present to the clinic at a later stage of disease. As the retina degenerates, inflammation and oxidative stress increase and trophic factor support declines
-
3WJ RNA Nanoparticles-Aptamer Functionalized Exosomes From M2 Macrophages Target BMSCs to Promote the Healing of Bone Fractures. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-11-03 Jiali Shou,Shuyi Li,Wenzhe Shi,Sijuan Zhang,Zheng Zeng,Zecong Guo,Ziming Ye,Zhuohao Wen,Huiguo Qiu,Jinheng Wang,Miao Zhou
Up to now, impaired bone regeneration severely affects the healing of bone fractures, thus bringing tremendous suffering to patients. As a vital mediator between inflammatory response and bone regeneration, M2 macrophage-derived exosomes (M2-Exos) attenuate inflammation and promote tissue repair. However, due to a lack of specific targeting property, M2-Exos will be rapidly eliminated after systematic
-
Ocular Surface Regeneration by Limbal Stem Cells Therapies: State of the Art, Challenges, and Perspectives. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-11-03 Clémence Bonnet,Sheyla González,Sophie X Deng
Limbal stem cells (LSCs) are adult stem cells located at the limbus ensuring the continuous renewal of the corneal epithelium, critical to maintain an optimal visual function. Damages to the LSCs or their niche microenvironment lead to limbal stem cell deficiency (LSCD), a potentially blinding disease. Transplantation of LSCs as a treatment for severe to total LSCD has gained popularity since 1980s
-
Attitudes Towards Standardization of Mesenchymal Stromal Cells-A Qualitative Exploration of Expert Views. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-11-03 Alison J Wilson,Nik Brown,Emma Rand,Paul G Genever
Pharmacopoeial standards ensure quality control of established medicines. It is widely believed that translation of cell therapy medicines will be facilitated by defining and adopting relevant standards. Mesenchymal stromal cells (MSCs) are used extensively for multiple indications in regenerative medicine. They are highly heterogeneous in terms of their biological characteristics and their mechanisms
-
Therapeutic Effects of Mesenchymal/Stromal Stem Cells and Their Derived Extracellular Vesicles in Rheumatoid Arthritis. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-11-02 Dimitrios Tsiapalis,Achilleas Floudas,Tobias Tertel,Verena Boerger,Bernd Giebel,Douglas J Veale,Ursula Fearon,Lorraine O'Driscoll
Currently available therapies for rheumatoid arthritis (RA) are inadequate to alleviate the inflammation and reduce joint damage. While the immune-regulatory effect of human mesenchymal/stromal stem cells (MSCs) extracellular vesicles (EVs) has been tested in many inflammation-related diseases, little is known regarding their effect on patients with RA. Thus, we assessed the effect of human MSCs and
-
Cord Blood Stem Cells Do Not "Age": Under Proper Banking Conditions. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-10-26 Andromachi Scaradavou
-
T-Cell Mediated Immune Rejection of Beta-2-Microglobulin Knockout Induced Pluripotent Stem Cell-Derived Kidney Organoids. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-10-16 Lonneke H Gaykema,Rianne Y van Nieuwland,Ellen Lievers,Wessel B J Moerkerk,Juliette A de Klerk,Sébastien J Dumas,Jesper Kers,Arnaud Zaldumbide,Cathelijne W van den Berg,Ton J Rabelink
Immune evasive induced pluripotent stem cell (iPSC)-derived kidney organoids, known as "stealth" organoids, hold promise for clinical transplantation. To address immune rejection, we investigated the impact of genetically modifying human leukocyte antigen (HLA) class I in kidney organoids prior to transplantation. By using CRISPR-Cas9, we successfully knocked out beta-2-microglobulin (B2M), resulting
-
Silicate Microfiber Scaffolds Support the Formation and Expansion of the Cortical Neuronal Layer of Cerebral Organoids With a Sheet-Like Configuration. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-10-16 Eisaku Terada,Yohei Bamba,Masatoshi Takagaki,Shuhei Kawabata,Tetsuro Tachi,Hajime Nakamura,Takeo Nishida,Haruhiko Kishima
Cerebral organoids (COs) are derived from human-induced pluripotent stem cells in vitro and mimic the features of the human fetal brain. The development of COs is largely dependent on "self-organization" mechanisms, in which differentiating cells committed to cortical cells autonomously organize into the cerebral cortex-like tissue. However, extrinsic manipulation of their morphology, including size
-
Autologous Airway Basal Cell Transplantation Alleviates Airway Epithelium Defect in Recurrent Benign Tracheal Stenosis. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-10-07 Yong-Shun Ye,Di-Fei Chen,Ming Liu,Yu-Long Luo,Huan-Jie Chen,Hai-Kang Zeng,Jing-Wei Liu,Yi-Ping Zhu,Xin-Yu Song,Chang-Qin Lin,Zhu-Quan Su,Shi-Yue Li
BACKGROUND Airway epithelium defects are a hallmark of recurrent benign tracheal stenosis (RBTS). Reconstructing an intact airway epithelium is of great importance in airway homeostasis and epithelial wound healing and has great potential for treating tracheal stenosis. METHODS An experimental study was conducted in canines to explore the therapeutic effect of autologous basal cell transplantation
-
Management of Retinitis Pigmentosa Via Wharton's Jelly-Derived Mesenchymal Stem Cells or Combination With Magnovision: 3-Year Prospective Results. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-10-05 Emin Ozmert,Umut Arslan
To investigate whether the natural progression rate of retinitis pigmentosa (RP) can be decreased with subtenon Wharton's jelly-derived mesenchymal stem cell (WJ-MSC) application alone or combination with Magnovision. The study included prospective analysis of 130 eyes of 80 retinitis pigmentosa patients with a 36-month follow-up duration. Patients constitute 4 groups with similar demographic characteristics
-
Mesenchymal Stem Cell Utilization for In Vitro Donor Liver Machine Perfusion Preservation: Current Status and Future Directions. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-10-05 Yu Fu,Yi Wang,Liwei Liang,Mingzi Gu,Yi Gao,Lei Feng
Liver transplantation is the only effective treatment for end-stage liver disease. Currently, the shortage of high-quality donors has led to the exploration of the use of marginal organs. However, several factors limit the in vitro long-term preservation and long-distance transport of livers, which can also lead to ischemia-reperfusion injuries, resulting in poor prognosis. Therefore, an efficient
-
External Application of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Hyaluronic Acid Gel Repairs Foot Wounds of Types I and II Diabetic Rats Through Paracrine Action Mode. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-10-05 Jingan Chen,Yi Liu,Jingwen Zhang,Yuping Yang,Haowei Liang,Ting Li,Li Yan,Li Zhou,Letian Shan,Hui Wang
Diabetic foot ulcer (DFU) is a main diabetic complication with unmet treatment needs. This study applied human umbilical cord-derived mesenchymal stem cells-hyaluronic acid (hucMSCs-HA) gel to treat DFU in a noninvasive external way and investigated its paracrine action and mechanism. In this study, after analyzing the physical and biological properties of HA gel, hucMSCs-HA gel was applied in 2 in
-
The Antioxidant TEMPOL Protects Human Hematopoietic Stem Cells From Culture-Mediated Loss of Functions. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-10-05 Elia Henry,Frédéric Picou,Vilma Barroca,Nathalie Dechamps,Steicy Sobrino,Emmanuelle Six,Camille Gobeaux,Patrick Auberger,Olivier Hérault,Françoise Pflumio,Marie-Laure Arcangeli
In a steady state, hematopoietic stem cells (HSC) exhibit very low levels of reactive oxygen species (ROS). Upon stress, HSC get activated and enter into proliferation and differentiation process to ensure blood cell regeneration. Once activated, their levels of ROS increase, as messengers to mediate their proliferation and differentiation programs. However, at the end of the stress episode, ROS levels
-
Allogeneic Cell Therapy Applications in Neonates: A Systematic Review. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-10-05 Abdul Razak,Donna Lei,Courtney A McDonald,Rod W Hunt,Suzanne L Miller,Atul Malhotra
BACKGROUND Neonatal cell therapy applications are increasing; however, data on allogeneic cell therapy are limited. OBJECTIVE To summarize evidence on allogeneic cell therapy in term and preterm neonates. METHODS Cochrane Central Register of Controlled Trials, Embase, Ovid Medline, and various registries were searched for studies investigating the safety, feasibility, and efficacy of allogeneic cell
-
Early Postnatal Expression of Tgfβ-1 and Fgf-2 Correlates With Regenerative Functions of Unrestricted Somatic Stem Cell Infusion After Rabbit GMH-IVH. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-09-29 Dina A Finkel,Ana Malfa,Yanling Liao,Deepti Purohit,Furong Hu,Davud Sulaymankhil,Sai Abhishek Narra,Karen Hussein,Selvakumar Subbian,Mitchell S Cairo,Govindaiah Vinukonda,Edmund F La Gamma
Intraventricular hemorrhage (IVH) is a severe complication of preterm birth associated with white matter injury (WMI) and reduced neurogenesis. IVH commonly arises from the germinal matrix, a highly cellular, transient structure, where all precursor cells are born, proliferate, and migrate during brain development. IVH leads to reduced progenitor cell proliferation and maturation and contributes to
-
Oxidative Stress and Neural Dysfunction in Gastrointestinal Diseases: Can Stem Cells Offer a Solution? Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-09-29 Rhian Stavely,Leah C Ott,Lauren Sahakian,Niloufar Rashidi,Samy Sakkal,Kulmira Nurgali
Oxidative stress is involved in many gastrointestinal (GI) disorders as either the primary pathogenesis (radiation, chemotherapy, toxicity, ischemia-reperfusion) or a secondary driving force of disease progression (inflammation and diabetes). The GI tract is innervated intrinsically by the enteric nervous system (ENS) with a diverse role in maintaining gut homeostasis and GI motility. Complications
-
Mechanisms of Breast Cancer Stem Cell Specification and Self-Renewal Mediated by Hypoxia-Inducible Factor 1. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-09-28 Gregg L Semenza
Many advanced human cancers contain regions of intratumoral hypoxia, with O2 gradients extending to anoxia. Hypoxia-inducible factors (HIFs) are activated in hypoxic cancer cells and drive metabolic reprogramming, vascularization, invasion, and metastasis. Hypoxia induces breast cancer stem cell (BCSC) specification by inducing the expression and/or activity of the pluripotency factors KLF4, NANOG
-
Efficacy of umbilical cord-derived mesenchymal stem cells in the treatment of type 2 diabetes assessed by retrospective continuous glucose monitoring. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-09-20 Li Zang,Yijun Li,Haojie Hao,Jiejie Liu,Qian Zhang,Fei Gao,Haibin Wang,Yulong Chen,Weijun Gu,Jin Du,Junhua Meng,Saichun Zhang,Zhaohui Lyu,Jingtao Dou,Yiming Mu
Umbilical cord-derived mesenchymal stem cells (UC-MSCs) have been proved a promising clinical strategy for the treatment of diabetes, and time in range (TIR) has been demonstrated a new metric of glycemic control links to diabetes complications. To further assess the therapeutic effect of UC-MSCs on TIR, a phase II study investigating the efficacy of UC-MSCs in Chinese adults with type 2 diabetes (T2D)
-
Preclinical Evidence for the Use of Oral Mesenchymal Stem Cell-Derived Extracellular Vesicles in Bone Regenerative Therapy: A Systematic Review. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-09-16 Allinson Olaechea,Karim Benabdellah,Andrea Vergara-Buenaventura,Sara Gómez-Melero,Emilio A Cafferata,Jonathan Meza-Mauricio,Miguel Padial-Molina,Pablo Galindo-Moreno
The development of extracellular vesicles (EVs) therapies has revolutionized personalized medicine, opening up new possibilities for treatment. EVs have emerged as a promising therapeutic tool within this field due to their crucial role in intercellular communication across various cell types and organisms. This systematic review aims to evaluate the therapeutic potential of oral mesenchymal stem cell
-
Human Spinal Oligodendrogenic Neural Progenitor Cells Enhance Pathophysiological Outcomes and Functional Recovery in a Clinically Relevant Cervical Spinal Cord Injury Rat Model. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-09-15 Katarzyna Pieczonka,Hiroaki Nakashima,Narihito Nagoshi,Kazuya Yokota,James Hong,Anna Badner,Jonathon C T Chio,Shinsuke Shibata,Mohamad Khazaei,Michael G Fehlings
Traumatic spinal cord injury (SCI) results in the loss of neurons, oligodendrocytes, and astrocytes. Present interventions for SCI include decompressive surgery, anti-inflammatory therapies, and rehabilitation programs. Nonetheless, these approaches do not offer regenerative solutions to replace the lost cells, fiber tracts, and circuits. Neural stem/progenitor cell (NPC) transplantation is a promising
-
Current Challenges and Future Promise for Use of Extracellular Matrix Scaffold to Achieve the Whole Organ Tissue Engineering Moonshot. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-09-15 Likitha Somasekhar,Leigh G Griffiths
Whole organ tissue engineering encompasses a variety of approaches, including 3D printed tissues, cell-based self-assembly, and cellular incorporation into synthetic or xenogeneic extracellular matrix (ECM) scaffolds. This review article addresses the importance of whole organ tissue engineering for various solid organ applications, focusing on the use of extracellular (ECM) matrix scaffolds in such
-
Long-Term Safety of Bone Regeneration Using Autologous Stromal Vascular Fraction and Calcium Phosphate Ceramics: A 10-Year Prospective Cohort Study. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-09-15 Vivian Wu,Jenneke Klein-Nulend,Nathalie Bravenboer,Christiaan M Ten Bruggenkate,Marco N Helder,Engelbert A J M Schulten
This prospective cohort study aimed to assess long-term safety, dental implant survival, and clinical and radiological outcomes after maxillary sinus floor elevation (MSFE; lateral window technique) using freshly isolated autologous stromal vascular fraction (SVF) combined with calcium phosphate ceramics. All 10 patients previously participating in a phase I trial were included in a 10-year follow-up
-
Orchestration of Mesenchymal Stem/Stromal Cells and Inflammation During Wound Healing. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-09-15 Mengting Zhu,Lijuan Cao,Sonia Melino,Eleonora Candi,Ying Wang,Changshun Shao,Gerry Melino,Yufang Shi,Xiaodong Chen
Wound healing is a complex process and encompasses a number of overlapping phases, during which coordinated inflammatory responses following tissue injury play dominant roles in triggering evolutionarily highly conserved principals governing tissue repair and regeneration. Among all nonimmune cells involved in the process, mesenchymal stem/stromal cells (MSCs) are most intensely investigated and have
-
Pluripotent Stem Cell-Derived In Vitro Gametogenesis and Synthetic Embryos-It Is Never Too Early for an Ethical Debate. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-09-15 Stefanie Horer,Michael Feichtinger,Margit Rosner,Markus Hengstschläger
Recently, 2 branches of the wide area of synthetic biology-in vitro gametogenesis and synthetic embryo development-have gained considerable attention. Rodent induced pluripotent stem cells derived via reprogramming of somatic cells can in vitro be differentiated into gametes to produce fertile offspring. And even synthetic embryos with organ progenitors were generated ex utero entirely from murine
-
Neural Cells for Neurodegenerative Diseases in Clinical Trials. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-08-16 Yiping Fan,Eyleen L K Goh,Jerry Kok Yen Chan
Neurodegenerative diseases (ND) are an entire spectrum of clinical conditions that affect the central and peripheral nervous system. There is no cure currently, with treatment focusing mainly on slowing down progression or symptomatic relief. Cellular therapies with various cell types from different sources are being conducted as clinical trials for several ND diseases. They include neural, mesenchymal
-
Progress in Stem Cells-Based Replacement Therapy for Retinal Pigment Epithelium: In Vitro Differentiation to In Vivo Delivery. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-08-16 Santosh Gupta,Lyubomyr Lytvynchuk,Taras Ardan,Hana Studenovska,Ruchi Sharma,Georgina Faura,Lars Eide,Rama Shanker Verma,Ljubo Znaor,Slaven Erceg,Knut Stieger,Jan Motlik,Goran Petrovski,Kapil Bharti
Retinal pigment epithelium (RPE) is a critical cell monolayer forming the blood-retina-barrier (BRB) and a permeable bridge between the choriocapillaris and the retina. RPE is also crucial in maintaining photoreceptor function and for completing the visual cycle. Loss of the RPE is associated with the development of degenerative diseases like age-related macular degeneration (AMD). To treat diseases
-
Aldehyde Dehydrogenase, a Marker of Normal and Malignant Stem Cells, Typifies Mesenchymal Progenitors in Perivascular Niches. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-07-14 Mario A Gomez-Salazar,Yiyun Wang,Neelima Thottappillil,Reef W Hardy,Manon Alexandre,Fabian Höller,Niall Martin,Zaniah N Gonzalez-Galofre,Dorota Stefancova,Daniele Medici,Aaron W James,Bruno Péault
Innate mesenchymal stem cells exhibiting multilineage differentiation and tissue (re)generative-or pathogenic-properties reside in perivascular niches. Subsets of these progenitors are committed to either osteo-, adipo-, or fibrogenesis, suggesting the existence of a developmental organization in blood vessel walls. We evaluated herein the activity of aldehyde dehydrogenase, a family of enzymes catalyzing
-
Fulfilling the Promise of RNA Therapies for Cardiac Repair and Regeneration. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-08-16 Mauro Giacca
The progressive appreciation that multiple types of RNAs regulate virtually all aspects of tissue function and the availability of effective tools to deliver RNAs in vivo now offers unprecedented possibilities for obtaining RNA-based therapeutics. For the heart, RNA therapies can be developed that stimulate endogenous repair after cardiac damage. Applications in this area include acute cardioprotection
-
Short-Term Clinical Response and Changes in the Fecal Microbiota and Metabolite Levels in Patients with Crohn's Disease After Stem Cell Infusions. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-08-16 Fan Yang,Xiaofang Zheng,Weicheng Liang,Beibei Ni,Jianxi Lu,Qiuli Liu,Ruixuan Xu,Yizhan He,Mary Miu Yee Waye,Qi Zhang,Yufeng Chen,Xiaoguang Zou,Wenjie Chen
Recent studies have shown a close relationship between the gut microbiota and Crohn's disease (CD). This study aimed to determine whether mesenchymal stem cell (MSC) treatment alters the gut microbiota and fecal metabolite pathways and to establish the relationship between the gut microbiota and fecal metabolites. Patients with refractory CD were enrolled and received 8 intravenous infusions of MSCs
-
Transplantation of hESCs-Derived Neural Progenitor Cells Alleviates Secondary Damage of Thalamus After Focal Cerebral Infarction in Rats. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-08-16 Kongping Li,Linhui Peng,Qi Xing,Xialin Zuo,Wenhao Huang,Lixuan Zhan,Heying Li,Weiwen Sun,Xiaofen Zhong,Tieshi Zhu,Guangjin Pan,En Xu
Human embryonic stem cells-derived neural progenitor cells (hESCs-NPCs) transplantation holds great potential to treat stroke. We previously reported that delayed secondary degeneration occurs in the ventroposterior nucleus (VPN) of ipsilateral thalamus after distal branch of middle cerebral artery occlusion (dMCAO) in adult male Sprague-Dawley (SD) rats. In this study, we investigate whether hESCs-NPCs
-
Direct Differentiation of Bone Marrow Mononucleated Cells Into Insulin-Producing Cells Using 4 Specific Soluble Factors. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-07-14 Seung-Ah Lee,Subin Kim,Seog-Young Kim,Jong Yoen Park,Jinyan Nan,Ho Seon Park,Hyunsuk Lee,Yong Deok Lee,Hakmo Lee,Shinae Kang,Hye Seung Jung,Sung Soo Chung,Kyong Soo Park
Bone marrow-derived stem cells are self-renewing and multipotent adult stem cells that differentiate into several types of cells. Here, we investigated a unique combination of 4 differentiation-inducing factors (DIFs), including putrescine (Put), glucosamine (GlcN), nicotinamide, and BP-1-102, to develop a differentiation method for inducing mature insulin-producing cells (IPCs) and apply this method
-
Extent of Tissue Washing Can Significantly Alter the Composition of Adipose-Derived Stromal Vascular Fraction Cell Preparations: Implications for Clinical Translation. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-06-15 Gabriela Aguilo-Seara,William Molair,Hulan Shang,Scott Northrup,Joshua A Grosser,Ramon Llull,Adam Katz
Stromal vascular fraction (SVF) cell preparations have recently attracted much interest as a form of autologous cell therapy. These heterogenous cell populations typically include some proportion of blood-derived cells (BDCs)-including both red blood cells (RBCs) and leukocytes (WBCs). The objectives of this paper were to evaluate the effects of tissue washing and hypotonic RBC lysis-separately and
-
Fate and Efficacy of Engineered Allogeneic Stem Cells Targeting Cell Death and Proliferation Pathways in Primary and Brain Metastatic Lung Cancer. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-07-14 Susana Moleirinho,Yohei Kitamura,Paulo S G N Borges,Sophia Auduong,Seyda Kilic,David Deng,Nobuhiko Kanaya,David Kozono,Jing Zhou,Jeffrey J Gray,Esther Revai-Lechtich,Yanni Zhu,Khalid Shah
Primary and metastatic lung cancer is a leading cause of cancer-related death and novel therapies are urgently needed. Epidermal growth factor receptor (EGFR) and death receptor (DR) 4/5 are both highly expressed in primary and metastatic non-small cell lung cancer (NSCLC); however, targeting these receptors individually has demonstrated limited therapeutic benefit in patients. In this study, we created
-
Mesenchymal Stem Cell-Extracellular Vesicle Therapy for Stroke: Scalable Production and Imaging Biomarker Studies. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-07-14 Jeong Pyo Son,Eun Hee Kim,Eun Kyoung Shin,Dong Hee Kim,Ji Hee Sung,Mi Jeong Oh,Jae Min Cha,Michael Chopp,Oh Young Bang
A major clinical hurdle to translate MSC-derived extracellular vesicles (EVs) is the lack of a method to scale-up the production of EVs with customized therapeutic properties. In this study, we tested whether EV production by a scalable 3D-bioprocessing method is feasible and improves neuroplasticity in animal models of stroke using MRI study. MSCs were cultured in a 3D-spheroid using a micro-patterned
-
Allogenic Umbilical Cord-Derived Mesenchymal Stromal Cells Sustain Long-Term Therapeutic Efficacy Compared With Low-Dose Interleukin-2 in Systemic Lupus Erythematosus. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-07-14 Zhouli Cao,DanDan Wang,Lijuan Jing,Xin Wen,Nan Xia,Wenjuan Ma,Xueyi Zhang,Ziyi Jin,Wei Shen,Genhong Yao,Weiwei Chen,Xiaojun Tang,Linyu Geng,Hui Li,Xiaojing Li,Shuai Ding,Jun Liang,Xuebing Feng,Huayong Zhang,Shanshan Liu,Wenchao Li,Lingyun Sun
OBJECTIVES Mesenchymal stromal cells (MSCs) and low-dose interleukin-2 (IL-2) both have demonstrated efficacy in treating systemic lupus erythematosus (SLE). The aim of this study is to conduct a head-to-head comparison between the 2 treatments and provide insights for clinical applications. METHODS Lupus-prone mice were treated with umbilical cord-derived MSCs (UC-MSCs), IL-2, or a combination of
-
Single-Cell RNA-Seq Reveals LRRC75A-Expressing Cell Population Involved in VEGF Secretion of Multipotent Mesenchymal Stromal/Stem Cells Under Ischemia. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-06-15 Takumi Miura,Tsukasa Kouno,Megumi Takano,Takuya Kuroda,Yumiko Yamamoto,Shinji Kusakawa,Masaki Suimye Morioka,Tohru Sugawara,Takamasa Hirai,Satoshi Yasuda,Rumi Sawada,Satoko Matsuyama,Hideya Kawaji,Takeya Kasukawa,Masayoshi Itoh,Akifumi Matsuyama,Jay W Shin,Akihiro Umezawa,Jun Kawai,Yoji Sato
Human multipotent mesenchymal stromal/stem cells (MSCs) have been utilized in cell therapy for various diseases and their clinical applications are expected to increase in the future. However, the variation in MSC-based product quality due to the MSC heterogeneity has resulted in significant constraints in the clinical utility of MSCs. Therefore, we hypothesized that it might be important to identify
-
Reprogramming of Acute Myeloid Leukemia Patients Cells: Harboring Cancer Mutations Requires Targeting of AML Hierarchy. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-06-15 Diana Golubeva,Deanna P Porras,Meaghan Doyle,Jennifer C Reid,Borko Tanasijevic,Allison L Boyd,Kinga Vojnits,Amro Elrafie,Amy Qiao,Mickie Bhatia
Screening of primary patient acute myeloid leukemia (AML) cells is challenging based on intrinsic characteristics of human AML disease and patient-specific conditions required to sustain AML cells in culture. This is further complicated by inter- and intra-patient heterogeneity, and "contaminating" normal cells devoid of molecular AML mutations. Derivation of induced pluripotent stem cells (iPSCs)
-
Propensity of Patient-Derived iPSCs for Retinal Differentiation: Implications for Autologous Cell Replacement. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-06-15 Jessica A Cooke,Andrew P Voigt,Michael A Collingwood,Nicholas E Stone,S Scott Whitmore,Adam P DeLuca,Erin R Burnight,Kristin R Anfinson,Christopher A Vakulskas,Austin J Reutzel,Heather T Daggett,Jeaneen L Andorf,Edwin M Stone,Robert F Mullins,Budd A Tucker
Prior to use, newly generated induced pluripotent stem cells (iPSC) should be thoroughly validated. While excellent validation and release testing assays designed to evaluate potency, genetic integrity, and sterility exist, they do not have the ability to predict cell type-specific differentiation capacity. Selection of iPSC lines that have limited capacity to produce high-quality transplantable cells
-
Transplantation of Human Brain-Derived Ischemia-Induced Multipotent Stem Cells Ameliorates Neurological Dysfunction in Mice After Stroke. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-06-15 Takayuki Nakagomi,Akiko Nakano-Doi,Shuji Kubo,Toshinori Sawano,Yoji Kuramoto,Kenichi Yamahara,Tomohiro Matsuyama,Toshinori Takagi,Nobutaka Doe,Shinichi Yoshimura
We recently demonstrated that injury/ischemia-induced multipotent stem cells (iSCs) develop within post-stroke human brains. Because iSCs are stem cells induced under pathological conditions, such as ischemic stroke, the use of human brain-derived iSCs (h-iSCs) may represent a novel therapy for stroke patients. We performed a preclinical study by transplanting h-iSCs transcranially into post-stroke
-
Metformin Improves Functional Outcomes, Activates Neural Precursor Cells, and Modulates Microglia in a Sex-Dependent Manner After Spinal Cord Injury. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-06-15 Emily A B Gilbert,Jessica Livingston,Emilio Garcia-Flores,Tarlan Kehtari,Cindi M Morshead
Spinal cord injury (SCI) results in devastating patient outcomes with few treatment options. A promising approach to improve outcomes following SCI involves the activation of endogenous precursor populations including neural stem and progenitor cells (NSPCs) which are located in the periventricular zone (PVZ), and oligodendrocyte precursor cells (OPCs) found throughout the parenchyma. In the adult
-
Correction of T-Cell Repertoire and Autoimmune Diabetes in NOD Mice by Non-myeloablative T-Cell Depleted Allogeneic HSCT. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-05-15 Rakefet Sidlik Muskatel,Bar Nathansohn-Levi,Shlomit Reich-Zeliger,Michal Mark,Liat Stoler-Barak,Chava Rosen,Irit Milman-Krentsis,Esther Bachar Lustig,Robert Pete Gale,Nir Friedman,Yair Reisner
The induction of partial tolerance toward pancreatic autoantigens in the treatment of type 1 diabetes mellitus (T1DM) can be attained by autologous hematopoietic stem cell transplantation (HSCT). However, most patients treated by autologous HSCT eventually relapse. Furthermore, allogeneic HSCT which could potentially provide a durable non-autoimmune T-cell receptor (TCR) repertoire is associated with
-
Three-Dimensional Spheroid Culture of Human Mesenchymal Stem Cells: Offering Therapeutic Advantages and In Vitro Glimpses of the In Vivo State. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-05-15 B Linju Yen,Chen-Chan Hsieh,Pei-Ju Hsu,Chia-Chi Chang,Li-Tzu Wang,Men-Luh Yen
As invaluable as the standard 2-dimensional (2D) monolayer in vitro cell culture system has been, there is increasing evidence that 3-dimensional (3D) non-adherent conditions are more relevant to the in vivo condition. While one of the criteria for human mesenchymal stem cells (MSCs) has been in vitro plastic adherence, such 2D culture conditions are not representative of in vivo cell-cell and cell-extracellular
-
Patient-Specific iPSC-Derived Models Link Aberrant Endoplasmic Reticulum Stress Sensing and Response to Juvenile Osteochondritis Dissecans Etiology. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-05-15 Giuliana E Salazar-Noratto,Catriana C Nations,Hazel Y Stevens,Maojia Xu,Sean Gaynard,Claire Dooley,Nica de Nijs,Katya McDonagh,Sanbing Shen,S Clifton Willimon,Frank Barry,Robert E Guldberg
Juvenile osteochondritis dissecans (JOCD) is a pediatric disease, which begins with an osteonecrotic lesion in the secondary ossification center which, over time, results in the separation of the necrotic fragment from the parent bone. JOCD predisposes to early-onset osteoarthritis. However, the knowledge gap in JOCD pathomechanisms severely limits current therapeutic strategies. To elucidate its etiology
-
Relationship Between Platelets and the Clinical Efficacy of Umbilical Cord Mesenchymal Stem Cells for HBV-Related Acute-on-Chronic Liver Failure and Liver Cirrhosis: A Preliminary Clinical Study. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-06-15 Ka Zhang,Yifan Jia,Xin Shu,Xiaoan Yang,Haixia Sun,Hong Cao,Yongyu Mei,Canhui Xiao
BACKGROUND Previous studies have found that the production of platelets could enhance the therapeutic effects of stem cells. Nevertheless, there are still no articles reporting on the relationship between platelets and the clinical efficacy of umbilical cord mesenchymal stem cells (UCMSCs) for HBV-related acute-on-chronic liver failure (ACLF) and liver cirrhosis (LC). METHODS In this retrospective
-
Expert Consideration on Regulatory Aspects for Perinatal Derivatives in Clinical Settings. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-05-15 Nicola Hofmann,Xavier Lafarge,Mariastefania Antica,Nicolas Ferry,Lenart Girandon,Roberto Gramignoli,Marcin Jurga,Halima Kerdjoudj,Ruta Navakauskiene,Jessica Schiavi,Volodymyr Shablii,Francisco J Nicolás,Florelle Gindraux
Perinatal derivatives (PnD) are drawing growing interest among the scientific community as an unrestricted source of multipotent stem cells, secretome, and biological matrices. They are useful for the treatment of diseases that currently have limited or no effective therapeutic options, but they require the development of regenerative approaches. With this development, the question of regulation of
-
Direct Lineage Reprogramming for Induced Keratinocyte Stem Cells: A Potential Approach for Skin Repair. Stem Cells Transl. Med. (IF 6.0) Pub Date : 2023-05-15 Haohui Lin,Yu Pan,Sa Cai
Severe trauma or chronic wounds can deplete the keratinocyte stem cells (KSCs) present in the epidermal basal layer or inhibit their migration leading to compromised wound healing. Supplementing KSCs is the key to solution while lineage reprogramming provides a new approach to acquiring KSCs. Through direct lineage reprogramming, induced KSCs (iKSCs) can be produced from somatic cells, which exhibit