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  • Revised subunit order of mammalian septin complexes explains their in-vitro polymerization properties
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-12-02
    Forooz Soroor; Moshe S. Kim; Oliva Palander; Yadu Balachandran; Richard F. Collins; Samir Benlekbir; John Rubinstein; William S. Trimble

    Septins are conserved GTP-binding cytoskeletal proteins that polymerize into filaments by end-to-end joining of heterooligomeric complexes. In human cells, both hexamers and octamers exist, and crystallography studies predicted the order of the hexamers to be SEPT7-SEPT6-SEPT2-SEPT2-SEPT6-SEPT7, while octamers are thought to have the same core, but with SEPT9 at the ends. However, based on this septin

    更新日期:2020-12-02
  • Mapping the electrostatic profile of cellular membranes
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-12-02
    Sharon Eisenberg; Ehud Haimov; Glenn F.W. Walpole; Jonathan Plumb; Michael M. Kozlov; Sergio Grinstein

    Anionic phospholipids can confer a net negative charge to biological membranes. This surface charge generates an electrical field that serves to recruit extrinsic cationic proteins, can alter the disposition of transmembrane proteins and causes the local accumulation of soluble counterions, altering the local pH and the concentration of physiologically important ions like calcium. Because the phospholipid

    更新日期:2020-12-02
  • The evolution of a cell biologist
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-11-30
    Jennifer Lippincott-Schwartz

    I am honored and humbled to receive the E. B. Wilson Medal and happy to share some reflections on my journey as a cell biologist. It took me a while to realize that my interest in biology would center on how cells are spatially and dynamically organized. From an initial fascination with cellular structures I came to appreciate that cells exhibit dynamism across all scales—from their molecules, to molecular

    更新日期:2020-12-01
  • Diversity through equity and inclusion: The responsibility belongs to all of us
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-11-30
    James A. Olzmann

    Despite the recognized benefits of diversity and the decades of programs targeted at increasing diversity in science, technology, engineering, mathematics, and medicine, the underrepresentation of historically excluded groups continues due to persisting systemic inequalities. It is imperative that we reassess our current recruitment strategies and reimagine our campus and workplace environments to

    更新日期:2020-12-01
  • A well-engineered path into cell biology and academia
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-11-30
    Anne E. Carpenter

    Winning the American Society for Cell Biology’s Women in Cell Biology Mid-career Award is incredibly meaningful to me, as it validates that someone focusing on engineering and applications can be a “real” cell biologist, too. Single-minded devotion to studying a particular biological process is not a prerequisite for a career in science and academia. The more diverse the scientific styles and demographics

    更新日期:2020-12-01
  • The burden of service for faculty of color to achieve diversity and inclusion: the minority tax
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-11-30
    JoAnn Trejo

    The exclusion of Blacks/African-Americans, Latinx/Hispanics, and Indigenous people from science has resulted in their underrepresentation in the biomedical workforce, especially in academia. Faculty diversity at academic institutions is unacceptably low (<6%) and has remained unchanged in the past 20 years. Despite low representation, faculty of color are disproportionately tasked with service to enhance

    更新日期:2020-12-01
  • Finding your unique path in science
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-11-30
    Prachee Avasthi

    I always found it curious that in science, we value unique, creative thinkers, but we teach scientists to progress in a formulaic manner that rarely takes each person’s individual strengths into account. Surprisingly, when we break the mold, we are often rewarded for it. This cycle of learning to survive using conventional wisdom but being rewarded for a unique path outside of it seems to be an unspoken

    更新日期:2020-12-01
  • Identification of new OPA1 cleavage site reveals that short isoforms regulate mitochondrial fusion
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-11-25
    Ruohan Wang; Prashant Mishra; Spiros D. Garbis; Annie Moradian; Michael J. Sweredoski; David C. Chan

    OPA1, a large GTPase of the dynamin superfamily, mediates fusion of the mitochondrial inner membranes, regulates cristae morphology, and maintains respiratory chain function. Inner-membrane-anchored long forms of OPA1 (l-OPA1) are proteolytically processed by the OMA1 or YME1L proteases, acting at cleavage sites S1 and S2 respectively, to produce short forms (s-OPA1). In both mouse and human, half

    更新日期:2020-11-27
  • Quantifying the Roles of Space and Stochasticity in Computer Simulations for Cell Biology and Cellular Biochemistry
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-11-25
    M. E. Johnson; A. Chen; J. R. Faeder; P. Henning; I. I. Moraru; M. Meier-Schellersheim; R. F. Murphy; T. Prüstel; J. A. Theriot; A. M. Uhrmacher

    Most of the fascinating phenomena studied in cell biology emerge from interactions among highly organized multi-molecular structures embedded into complex and frequently dynamic cellular morphologies. For the exploration of such systems, computer simulation has proved to be an invaluable tool, and many researchers in this field have developed sophisticated computational models for application to specific

    更新日期:2020-11-27
  • pHLARE: A New Biosensor Reveals Decreased Lysosome pH in Cancer Cells
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-11-25
    Bradley A. Webb; Francesca M. Aloisio; Rabab A. Charafeddine; Jessica Cook; Torsten Wittmann; Diane L. Barber

    Many lysosome functions are determined by a lumenal pH of ∼5.0, including the activity of resident acid-activated hydrolases. Lysosome pH (pHlys) is often increased in neurodegenerative disorders and predicted to be decreased in cancers, making it a potential target for therapeutics to limit the progression of these diseases. Accurately measuring pHlys, however, is limited by currently used dyes that

    更新日期:2020-11-27
  • APC regulation of ESRP1 and p120-catenin isoforms in colorectal cancer cells
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-11-25
    Maree C. Faux; Lauren E. King; Serena R. Kane; Christopher Love; Oliver M. Sieber; Antony W. Burgess

    The APC tumor suppressor protein is associated with the regulation of Wnt signaling, however APC also controls other cellular processes including the regulation of cell adhesion and migration. The expression of full-length APC in SW480 colorectal cancer cells (SW480+APC) not only reduces Wnt signaling, but increases membrane E-cadherin and restores cell-cell adhesion. This report describes the effects

    更新日期:2020-11-27
  • Elevating PI3P drives select downstream membrane trafficking pathways
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-11-25
    Noah Steinfeld; Vikramjit Lahiri; Anna Morrison; Shree Padma Metur; Daniel J. Klionsky; Lois S. Weisman

    Phosphoinositide signaling lipids are essential for several cellular processes. The requirement for a phosphoinositide is conventionally studied by depleting the corresponding lipid kinase. However, there are very few reports on the impact of elevating phosphoinositides. That phosphoinositides are dynamically elevated in response to stimuli suggests that, in addition to being required, phosphoinositides

    更新日期:2020-11-27
  • Unconventional endocytosis and trafficking of Transferrin Receptor induced by iron
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-11-25
    Elena Gammella; Irene Schiano Lomoriello; Alexia Conte; Stefano Freddi; Alessandra Alberghini; Maura Poli; Sara Sigismund; Gaetano Cairo; Stefania Recalcati

    The post-translational regulation of transferrin receptor (TfR1) is largely unknown. We investigated whether iron availability affects TfR1 endocytic cycle and protein stability in HepG2 hepatoma cells exposed to ferric ammonium citrate (FAC). NH4Cl and bafilomycin A1, but not the proteasomal inhibitor MG132, prevented the FAC-mediated decrease in TfR1 protein levels, thus indicating lysosomal involvement

    更新日期:2020-11-27
  • Whole-genome screen identifies diverse pathways that negatively regulate ciliogenesis
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-11-18
    Marion Failler; Ariadna Giro-Perafita; Mikito Owa; Shalini Srivastava; Chi Yun; David J. Kahler; Derya Unutmaz; Francisco J. Esteva; Irma Sánchez; Brian D. Dynlacht

    We performed a high-throughput whole-genome RNAi screen to identify novel inhibitors of ciliogenesis in normal and basal breast cancer cells. Our screen uncovered a previously undisclosed, extensive network of genes linking integrin signaling and cellular adhesion to the extracellular matrix with inhibition of ciliation in both normal and cancer cells. Surprisingly, a cohort of genes encoding extracellular

    更新日期:2020-11-19
  • #BlackAndFemaleAndSTEM
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-11-12

    The #MeToo movement heightened awareness of sexism in science. More recently, nationwide protests against police brutality and other expressions of systemic anti-Black racism triggered new attention to racism in science. But without an intersectional approach, the realities for Black women can be overlooked. Using my own experience as a Black female PhD student, I argue that institutional attitudes

    更新日期:2020-11-13
  • Challenges and advice for MD/PhD applicants who are underrepresented in medicine
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-11-12
    Carl Bannerman; Natalie Guzman; Rachit Kumar; Chelsea Nnebe; Jordan Setayesh; Amitej Venapally; Jonathan H. Sussman

    The importance of diversity is self-evident in medicine and medical research. Not only does diversity result in more impactful scientific work, but diverse teams of researchers and clinicians are necessary to address health disparities and improve the health of underserved communities. MD/PhD programs serve an important role in training physician-scientists, so it is critical to ensure that MD/PhD

    更新日期:2020-11-13
  • Alternate histories of cytokinesis: lessons from the trypanosomatids
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-11-12
    Paul C. Campbell; Christopher L. de Graffenried

    Popular culture has recently produced several “alternate histories” that describe worlds where key historical events had different outcomes. Beyond entertainment, asking “could this have happened a different way?” and “what would the consequences be?” are valuable approaches for exploring molecular mechanisms in many areas of research, including cell biology. Analogous to alternate histories, studying

    更新日期:2020-11-13
  • Calcium spikes accompany the cleavage furrow ingression and cell separation during fission yeast cytokinesis
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-11-11
    Abhishek Poddar; Oumou Sidibe; Aniruddha Ray; Qian Chen

    The role of calcium signaling during cytokinesis has long remained ambiguous. Past studies of embryonic cell division discovered that calcium concentration increases transiently at the division plane just before the cleavage furrow ingression, suggesting that these calcium transients could trigger the contractile ring constriction. However, such calcium transients have only been found in animal embryos

    更新日期:2020-11-12
  • EWSR1 affects PRDM9-dependent histone 3 methylation and provides a link between recombination hotspots and the chromosome axis protein REC8
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-11-11
    Hui Tian; Timothy Billings; Petko M. Petkov

    Meiotic recombination in most mammals requires recombination hotspot activation through the action of the histone 3 lysine-4 and lysine-36 methyltransferase PRDM9 to ensure successful double-strand break initiation and repair. Here we show that EWSR1, a protein whose role in meiosis was not previously clarified in detail, binds to both PRDM9 and pREC8, a phosphorylated meiosis-specific cohesin, in

    更新日期:2020-11-12
  • Cooperation of the IFT-A complex with the IFT-B complex is required for ciliary retrograde protein trafficking and GPCR import
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-11-11
    Takuya Kobayashi; Yamato Ishida; Tomoaki Hirano; Yohei Katoh; Kazuhisa Nakayama

    Cilia sense and transduce extracellular signals via specific receptors. The intraflagellar transport (IFT) machinery mediates not only bidirectional protein trafficking within cilia but also the import/export of ciliary proteins across the ciliary gate. The IFT machinery is known to comprise two multisubunit complexes, namely, IFT-A and IFT-B; however, little is known about how the two complexes cooperate

    更新日期:2020-11-12
  • Insulin promoted mobilization of GLUT4 from a perinuclear storage site requires RAB10.
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-11-11
    Alexandria Brumfield; Natasha Chaudhary; Dorothee Molle; Jennifer Wen; Johannes Graumann; Timothy E. McGraw

    Insulin controls glucose uptake into muscle and fat cells by inducing a net redistribution of GLUT4 from intracellular storage to the plasma membrane (PM). The TBC1D4-RAB10 signaling module is required for insulin-stimulated GLUT4 translocation to the PM, although where it intersects GLUT4 traffic was unknown. Here we demonstrate that TBC1D4-RAB10 functions to control GLUT4 mobilization from a Trans

    更新日期:2020-11-12
  • Constitutive Musashi1 expression impairs mouse postnatal development and intestinal homeostasis
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-11-11
    Thelma T. Chiremba; Kristi L. Neufeld

    Evolutionarily conserved RNA-binding protein Musashi1 (Msi1) can regulate developmentally relevant genes. Here we report the generation and characterization of a mouse model that allows inducible Msi1 overexpression in a temporal and tissue-specific manner. We show that ubiquitous Msi1 induction in ∼5 week old mice delays overall growth, alters organ-to-body proportions, and causes premature death

    更新日期:2020-11-12
  • R-loops at centromeric chromatin contribute to defects in kinetochore integrity and chromosomal instability in budding yeast
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-11-04
    Prashant K. Mishra; Arijita Chakraborty; Elaine Yeh; Wenyi Feng; Kerry S. Bloom; Munira A. Basrai

    R-loops, the by-product of DNA-RNA hybridization and the displaced single stranded DNA, have been identified in bacteria, yeasts, and other eukaryotic organisms. The persistent presence of R-loops contributes to defects in DNA replication and repair, gene expression, and genomic integrity. R-loops have not been detected at centromeric (CEN) chromatin in wild-type budding yeast. Here we used an hpr1∆ strain

    更新日期:2020-11-04
  • Guanine Nucleotide Binding Protein like-1 (GNL1) Promotes Cancer Cell Proliferation and Survival through AKT/p21CIP1 Signaling Cascade
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-11-04
    Rehna Krishnan; Mariappan Murugiah; Naga Padma Lakshmi; Sundarasamy Mahalingam

    Human Guanine nucleotide binding protein like 1 (GNL1) is an evolutionary conserved putative nucleolar GTPase belonging to HSR1_MMR1 subfamily of GTPases. GNL1 was found to be highly upregulated in various cancers. Here, we report for the first time that GNL1 inhibits apoptosis by modulating the expression of Bcl2 family of proteins and the cleavage of caspases 7 and 8. Furthermore, GNL1 protects colon

    更新日期:2020-11-04
  • PTP-3 phosphatase promotes intramolecular folding of SYD-2 to inactivate kinesin-3 UNC-104 in neurons
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-11-04
    Muniesh Muthaiyan Shanmugam; Syed Nooruzuha Barmaver; Hsin-Yi Huang; Odvogmed Bayansan; Oliver Ingvar Wagner

    UNC-104 is the C. elegans homolog of kinesin-3 KIF1A known for its fast shuffling of STVs (synaptic vesicle protein transport vesicles) in axons. SYD-2 is the homolog of liprin-α in C. elegans known to activate UNC-104, however, signals that trigger SYD-2 binding to the motor remain unknown. Because SYD-2 is a substrate of PTP-3/LAR PTPR, we speculate a role of this phosphatase in SYD-2-mediated motor

    更新日期:2020-11-04
  • Coordination of NMCP1- and NMCP2-class proteins within the plant nucleoskeleton
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-11-04
    Endia L. Blunt; Jason A. Shandler; Erika J. Hughes; Hayley Sussman; Rachel C. Christopherson; Eric J. Richards

    Plants lack lamin proteins but contain a class of coiled-coil proteins that serve as analogs to form a laminal structure at the nuclear periphery. These Nuclear Matrix Constituent Proteins (NMCPs) play important roles in regulating nuclear morphology and are partitioned into two distinct groups. We investigated Arabidopsis NMCPs (called CRWNs) to study the inter-relationship between the three NMCP1-type

    更新日期:2020-11-04
  • ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-10-28
    Jason C. Casler; Allison L. Zajac; Fernando M. Valbuena; Daniela Sparvoli; Okunola Jeyifous; Aaron P. Turkewitz; Sally Horne-Badovinac; William N. Green; Benjamin S. Glick

    Membrane traffic can be studied by imaging a cargo protein as it transits the secretory pathway. The best tools for this purpose initially block export of the secretory cargo from the endoplasmic reticulum (ER), and then release the block to generate a cargo wave. However, previously developed regulatable secretory cargoes are often tricky to use or specific for a single model organism. To overcome

    更新日期:2020-10-30
  • Asymmetric chromosome segregation and cell division in DNA damage-induced bacterial filaments
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-10-28
    Suchitha Raghunathan; Afroze Chimthanawala; Sandeep Krishna; Anthony G. Vecchiarelli; Anjana Badrinarayanan

    Faithful propagation of life requires coordination of DNA replication and segregation with cell growth and division. In bacteria, this results in cell size homeostasis and periodicity in replication and division. The situation is perturbed under stress such as DNA damage, which induces filamentation as cell cycle progression is blocked to allow for repair. Mechanisms that release this morphological

    更新日期:2020-10-30
  • Cdc1p is a Golgi-localized Glycosylphosphatidylinositol-anchored Protein Remodelase
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-10-28
    Gege Yang; David K. Banfield

    Glycosylphosphatidylinositol-anchored proteins (GPI-APs) undergo extensive post-translational modifications and remodeling including the addition and subsequent removal of phospho-ethanolamine (EtNP) from mannose 1 (Man1) and mannose 2 (Man2) of the glycan moiety. Removal of EtNP from Man1 is catalyzed by Cdc1p, an event that has previously been considered to occur in the ER. We establish that Cdc1p

    更新日期:2020-10-30
  • A 20-year encounter with the imposter syndrome
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-10-29
    Thoru Pederson

    Both in their formative years and later careers, some scientists suffer from something more than occasional self-doubts. There is a more severe affliction that strikes many more than was once realized. Here I reflect on my encounter, in the hope that sharing it can be of some value.

    更新日期:2020-10-30
  • Beyond Langmuir: surface-bound macromolecule condensates
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-10-29
    T. J. Mitchison

    Macromolecule condensates, phase separation, and membraneless compartments have become an important area of cell biology research where new biophysical concepts are emerging. This article discusses the possibility that condensates assemble on multivalent surfaces such as DNA, microtubules, or lipid bilayers by multilayer adsorption. Langmuir isotherm theory conceptualized saturable surface binding

    更新日期:2020-10-30
  • Scientific societies fostering inclusivity through speaker diversity in annual meeting programming: a call to action
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-10-29
    Verónica A. Segarra; Clara Primus; Graciela A. Unguez; Ashanti Edwards; Candice Etson; Sonia C. Flores; Catherine Fry; Ashley N. Guillory; Susan L. Ingram; Mark Lawson; Richard McGee; Stephanie Paxson; Laura Phelan; Kirsta Suggs; Leticia R. Vega; Elizabeth Vuong; J. Christopher Havran; Alfredo Leon; Michael D. Burton; J. Luis Lujan; Marina Ramirez-Alvarado

    Scientific societies aiming to foster inclusion of scientists from underrepresented (UR) backgrounds among their membership often delegate primary responsibility for this goal to a diversity-focused committee. The National Science Foundation has funded the creation of the Alliance to Catalyze Change for Equity in STEM Success (ACCESS), a meta-organization bringing together representatives from several

    更新日期:2020-10-30
  • A quantitative and semi-automated method for determining misaligned and lagging chromosomes during mitosis
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-10-21
    Luciano Gama Braga; Diogjena Katerina Prifti; Chantal Garand; Pawan Kumar Saini; Sabine Elowe

    Accurate chromosome alignment at metaphase facilitates the equal segregation of sister chromatids to each of the nascent daughter cells. Lack of proper metaphase alignment is an indicator of defective chromosome congression and aberrant kinetochore-microtubule attachments which in turn promotes chromosome missegregation and aneuploidy, hallmarks of cancer. Tools to sensitively, accurately and quantitatively

    更新日期:2020-10-27
  • Structurally distinct endocytic pathways for B cell receptors in B lymphocytes
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-10-21
    Aleah D. Roberts; Thaddeus M. Davenport; Andrea M. Dickey; Regina Ahn; Kem A. Sochacki; Justin W. Taraska

    B lymphocytes play a critical role in adaptive immunity. Upon antigen binding, B cell receptors (BCR) cluster on the plasma membrane and are internalized by endocytosis. In this process, B cells capture diverse antigens in various contexts and concentrations. However, it is unclear whether the mechanism of BCR endocytosis changes in response to these factors. Here, we studied the mechanism of soluble

    更新日期:2020-10-27
  • Two high-yield complementary methods to sort cell populations by their 2D or 3D migration speed
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-10-21
    Aditya Arora; Jorge Luis Galeano Niño; Myint Zu Myaing; Shumei Chia; Bakya Arasi; Andrea Ravasio; Ruby Yun-Ju Huang; Ramanuj Dasgupta; Maté Biro; Virgile Viasnoff

    The potential to migrate is one of the most fundamental functions for various epithelial, mesenchymal and immune cells. Image analysis of motile cell populations, both primary and cultured, typically reveals an intercellular variability in migration speeds. However, cell migration chromatography, the sorting of large populations of cells based on their migratory characteristics, cannot be easily performed

    更新日期:2020-10-27
  • Pedagogy of the Black academic
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-10-15
    Glenn E. Simmons

    I am just starting my career as a cancer biologist, but I have always been a Black man in America. This means that I have always inhabited a world that generally disregarded my existence in some form or another. It is June 17th, 2020 and protests have been happening for weeks since the killing of George Floyd in Minneapolis. The current state of America may be uneasy for some, but for many Americans

    更新日期:2020-10-16
  • Ciliate behavior: blueprints for dynamic cell biology and microscale robotics
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-10-15
    Scott M. Coyle

    Place a drop of pond water under the microscope, and you will likely find an ocean of extraordinary and diverse single-celled organisms called ciliates. This remarkable group of single-celled organisms wield microtubules, active systems, electrical signaling, and chemical sensors to build intricate geometrical structures and perform complex behaviors that can appear indistinguishable from those of

    更新日期:2020-10-16
  • Developing a culture of safety in biomedical research training
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-10-15
    Michelle R. Bond; Alison E. Gammie; Jon R. Lorsch

    The National Institute of General Medical Sciences (NIGMS) at the U.S. National Institutes of Health (NIH) is committed to supporting the safety of the nation’s biomedical research and training environments. Institutional training grants affect many trainees and can have a broad influence across their parent institutions, making them good starting points for our initial efforts to promote the development

    更新日期:2020-10-16
  • Non-muscle myosin-2 contractility-dependent actin turnover limits the length of epithelial microvilli
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-10-07
    Colbie R. Chinowsky; Julia A. Pinette; Leslie M. Meenderink; Ken S. Lau; Matthew J. Tyska

    Brush border microvilli enable functions that are critical for epithelial homeostasis, including solute uptake and host defense. However, mechanisms that regulate the assembly and morphology of these protrusions are poorly understood. The parallel actin bundles that support microvilli have their pointed-end rootlets anchored in a filamentous meshwork referred to as the “terminal web.” Although classic

    更新日期:2020-10-07
  • Regulation of meiotic progression by Sertoli-cell androgen signaling
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-10-07
    Hailey Larose; Travis Kent; Qianyi Ma; Adrienne Niederriter Shami; Nadia Harerimana; Jun Z. Li; Saher Sue Hammoud; Mary Ann Handel

    Androgen receptor (AR) signaling in Sertoli cells is known to be important for germ-cell progression through meiosis, but the extent to which androgens indirectly regulate specific meiotic stages is not known. Here, we combine synchronization of spermatogenesis, cytological analyses and single-cell RNAseq (scRNAseq) in the Sertoli cell androgen receptor knockout (SCARKO) mutant and control mice, and

    更新日期:2020-10-07
  • Coordination of Grp1 recruitment mechanisms by its phosphorylation
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-10-07
    Jian Li; David G. Lambright; Victor W. Hsu

    The action of guanine nucleotide exchange factors (GEFs) on the ADP-Ribosylation Factor (ARF) family of small GTPases initiates intracellular transport pathways. This role requires ARF GEFs to be recruited from the cytosol to intracellular membrane compartments. An ARF GEF known as General receptor for 3-phosphoinositides 1 (Grp1) is recruited to the plasma membrane through its pleckstrin homology

    更新日期:2020-10-07
  • RAC1 induces nuclear alterations through the LINC complex to enhance melanoma invasiveness
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-10-07
    Paula Colón-Bolea; Rocío García -Gómez; Sue Shackleton; Piero Crespo; Xosé R. Bustelo; Berta Casar

    RHO GTPases are key regulators of the cytoskeletal architecture, which impacts on a broad range of biological processes in malignant cells including motility, invasion and metastasis, thereby affecting tumor progression. One of the constrains during cell migration, is the diameter of the pores through which cells pass. In this respect, the size and shape of the nucleus poses a major limitation. Therefore

    更新日期:2020-10-07
  • Microtubule-dependent pushing forces contribute to long-distance aster movement and centration in Xenopus laevis egg extracts
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-10-07
    Taylor Sulerud; Abdullah Bashar Sami; Guihe Li; April Kloxin; John Oakey; Jesse Gatlin

    During interphase of the eukaryotic cell cycle, the microtubule (MT) cytoskeleton serves as both a supportive scaffold for organelles and an arborized system of tracks for intracellular transport. At the onset of mitosis, the position of the astral MT network, specifically its center, determines the eventual location of the spindle apparatus and ultimately the cytokinetic furrow. Positioning of the

    更新日期:2020-10-07
  • More than just a ticket canceller: The mitochondrial processing peptidase tailors complex precursor proteins at internal cleavage sites
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-09-30
    Jana Friedl; Michael R. Knopp; Carina Groh; Eyal Paz; Sven B. Gould; Johannes M. Herrmann; Felix Boos

    Most mitochondrial proteins are synthesized as precursors that carry N-terminal presequences. After import into mitochondria, these targeting signals are cleaved off by the mitochondrial processing peptidase MPP. Using the mitochondrial tandem protein Arg5,6 as model substrate, we demonstrate that MPP has an additional role in preprotein maturation, beyond the removal of presequences. Arg5,6 is synthesized

    更新日期:2020-10-02
  • Ase1 domains dynamically slow anaphase spindle elongation and recruit Bim1 to the midzone
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-09-30
    Ezekiel C. Thomas; Amber Ismael; Jeffrey K. Moore

    How cells regulate microtubule crosslinking activity to control the rate and duration of spindle elongation during anaphase is poorly understood. In this study, we test the hypothesis that PRC1/Ase1 proteins use distinct microtubule-binding domains to control spindle elongation rate. Using budding-yeast Ase1, we identify unique contributions for the spectrin and carboxy-terminal domains during different

    更新日期:2020-10-02
  • LIM and SH3 Protein 1 Localizes to the Leading Edge of Protruding Lamellipodia and Regulates Axon Development
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-09-30
    Stephanie L Pollitt; Kenneth R Myers; Jin Yoo; James Q Zheng

    The actin cytoskeleton drives cell motility and is essential for neuronal development and function. LIM and SH3 Protein 1 (LASP1) is a unique actin-binding protein that is expressed in a wide range of cells including neurons, but its roles in cellular motility and neuronal development are not well understood. We report that LASP1 is expressed in rat hippocampus early in development, and this expression

    更新日期:2020-10-02
  • DNA Content Contributes to Nuclear Size Control in Xenopus laevis
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-09-30
    Hiroko Heijo; Sora Shimogama; Shuichi Nakano; Anna Miyata; Yasuhiro Iwao; Yuki Hara

    Cells adapt to drastic changes in genome quantity during evolution and cell division by adjusting the nuclear size to exert genomic functions. However, the mechanism by which DNA content within the nucleus contributes to controlling the nuclear size, remains unclear. Here, we experimentally evaluated the effects of DNA content by utilizing cell-free Xenopus egg extracts and imaging of in vivo embryos

    更新日期:2020-10-02
  • Masters of asymmetry – lessons and perspectives from 50 years of septins
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-09-29
    Elias T. Spiliotis; Michael A. McMurray

    Septins are a unique family of GTPases, which were discovered 50 years ago as essential genes for the asymmetric cell shape and division of budding yeast. Septins assemble into filamentous nonpolar polymers, which associate with distinct membrane macrodomains and subpopulations of actin filaments and microtubules. While structurally a cytoskeleton-like element, septins function predominantly as spatial

    更新日期:2020-09-30
  • Direct involvement of Hsp70 ATP hydrolysis in Ubr1-dependent quality control.
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-09-23
    Amanjot Singh,Nidhi Vashistha,Jarrod Heck,Xin Tang,Peter Wipf,Jeffrey L Brodsky,Randolph Y Hampton

    Chaperones can mediate both protein folding and degradation. This process is referred to as protein triage, which demands study to reveal mechanisms of quality control for both basic scientific and translational purposes. In yeast, many misfolded proteins undergo chaperone dependent ubiquitination by the action of E3 ligases Ubr1 and San1, allowing detailed study of protein triage. In cells, both HSP70

    更新日期:2020-09-23
  • SLC45A2 protein stability and regulation of melanosome pH determine melanocyte pigmentation.
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-09-23
    Linh Le,Iliana E Escobar,Tina Ho,Ariel J Lefkovith,Emily Latteri,Kirk D Haltaufderhyde,Megan K Dennis,Lynn Plowright,Elena V Sviderskaya,Dorothy C Bennett,Elena Oancea,Michael S Marks

    SLC45A2 encodes a putative transporter expressed primarily in pigment cells. SLC45A2 mutations cause oculocutaneous albinism type IV (OCA4) and polymorphisms are associated with pigmentation variation, but the localization, function, and regulation of SLC45A2 and its variants remain unknown. We show that SLC45A2 localizes to a cohort of mature melanosomes that only partially overlaps with those expressing

    更新日期:2020-09-23
  • Growth disadvantage associated with centrosome amplification drives population-level centriole number homeostasis.
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-09-23
    Roberta Sala,K C Farrell,Tim Stearns

    The centriole duplication cycle normally ensures that centriole number is maintained at two centrioles per G1 cell. However, some circumstances can result in an aberrant increase in centriole number—a phenotype that is particularly prevalent in several types of cancer. Following an artificial increase in centriole number without tetraploidization due to transient overexpression of the kinase PLK4,

    更新日期:2020-09-23
  • Yeast Rgd3 is a phospho-regulated F-BAR-containing RhoGAP involved in the regulation of Rho3 distribution and cell morphology.
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-09-17
    Robert M Gingras,Kyaw Myo Lwin,Abigail M Miller,Anthony Bretscher

    Polarized growth requires the integration of polarity pathways with the delivery of exocytic vesicles for cell expansion and counterbalancing endocytic uptake. In budding yeast, the myosin-V Myo2 is aided by the kinesin-related protein Smy1 in carrying out the essential Sec4-dependent transport of secretory vesicles to sites of polarized growth. Over-expression suppressors of a conditional myo2 smy1

    更新日期:2020-09-18
  • Beyond the bench: how inclusion and exclusion make us the scientists we are.
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-09-14
    Christina M Termini,Amara Pang

    Recent events encompassing social injustices, healthcare disparities, and police brutality against Black citizens highlight the continued need to strive toward unbiased and inclusive practices in all realms of the world. Our voices as cell biologists are powerful tools that can be used to combat inequities in the scientific landscape. In this inaugural Voices essay, we discuss how exclusion and inclusion

    更新日期:2020-09-15
  • Regulation of NRF1, a master transcription factor of proteasome genes: implications for cancer and neurodegeneration.
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-09-14
    Amy Northrop,Holly A Byers,Senthil K Radhakrishnan

    The ability to sense proteasome insufficiency and respond by directing the transcriptional synthesis of de novo proteasomes is a trait that is conserved in evolution and is found in organisms ranging from yeast to humans. This homeostatic mechanism in mammalian cells is driven by the transcription factor NRF1. Interestingly, NRF1 is synthesized as an endoplasmic reticulum (ER) membrane protein and

    更新日期:2020-09-15
  • Introducing MBoC Voices.
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-09-15
    Matthew D Welch

    更新日期:2020-09-15
  • Src kinases relax adherens junctions between the neighbors of apoptotic cells to permit apical extrusion.
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-09-09
    Jessica L Teo,Vanesa M Tomatis,Luke Coburn,Anne K Lagendijk,Irin-Maya Schouwenaar,Srikanth Budnar,Thomas E Hall,Suzie Verma,Robert W McLachlan,Benjamin M Hogan,Robert G Parton,Alpha S Yap,Guillermo A Gomez

    Epithelia can eliminate apoptotic cells by apical extrusion. This is a complex morphogenetic event where expulsion of the apoptotic cell is accompanied by rearrangement of its immediate neighbors to form a rosette. A key mechanism for extrusion is constriction of an actomyosin network that neighbor cells form at their interface with the apoptotic cell. Here we report a complementary process of cytoskeletal

    更新日期:2020-09-10
  • Telomere length set point regulation in human pluripotent stem cells critically depends on the shelterin protein TPP1.
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-09-09
    John M Boyle,Kelsey M Hennick,Samuel G Regalado,Jacob M Vogan,Xiaozhu Zhang,Kathleen Collins,Dirk Hockemeyer

    Telomere maintenance is essential for the long-term proliferation of human pluripotent stem cells, while their telomere length set point determines the proliferative capacity of their differentiated progeny. The shelterin protein TPP1 is required for telomere stability and elongation, but its role in establishing a telomere length set point remains elusive. Here, we characterize the contribution of

    更新日期:2020-09-10
  • PICH regulates the abundance and localization of SUMOylated proteins on mitotic chromosomes.
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-09-02
    Victoria A Hassebroek,Hyewon Park,Nootan Pandey,Brooklyn T Lerbakken,Vasilisa Aksenova,Alexei Arnaoutov,Mary Dasso,Yoshiaki Azuma

    Proper chromosome segregation is essential for faithful cell division and if not maintained results in defective cell function caused by abnormal distribution of genetic information. Polo-like kinase 1 interacting checkpoint helicase (PICH) is a DNA translocase essential for chromosome bridge resolution during mitosis. Its function in resolving chromosome bridges requires both DNA translocase activity

    更新日期:2020-09-03
  • β-actin contributes to an open chromatin for activation of the adipogenic pioneer factor CEBPA during transcriptional reprograming.
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-09-02
    M A Al-Sayegh,S R Mahmood,S B Abul Khair,X Xie,M El Gindi,T Kim,A Almansoori,P Percipalle

    Adipogenesis is regulated by a cascade of signals that drive transcriptional reprogramming in adipocytes. Here, we report that nuclear actin regulates the chromatin states that establish tissue specific expression during adipogenesis. To study the role of β-actin in adipocyte differentiation we conducted RNA-Sequencing on wild-type (WT) and β-actin knockout (KO) mouse embryonic fibroblasts (MEFs) after

    更新日期:2020-09-03
  • The prion-like domain of FUS is phosphorylated by multiple kinases affecting liquid- and solid-phase transitions.
    Mol. Biol. Cell (IF 3.791) Pub Date : 2020-09-02
    Izzy Owen,Shannon Rhoads,Debra Yee,Hala Wyne,Kevin Gery,Isabelle Hannula,Meenakshi Sundrum,Frank Shewmaker

    Fused in Sarcoma (FUS) is a ubiquitously expressed protein that can phase-separate from nucleoplasm and cytoplasm into distinct liquid-droplet structures. It is predominately nuclear and most of its functions are related to RNA and DNA metabolism. Excessive persistence of FUS within cytoplasmic phase-separated assemblies is implicated in the diseases amyotrophic lateral sclerosis (ALS) and frontotemporal

    更新日期:2020-09-02
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