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Validation of CSF-1 receptor (CD115) staining for analysis of murine monocytes by flow cytometry. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-12-01 Tamar Kapanadze,Jaba Gamrekelashvili,Stefan Sablotny,Frauline Nicole Schroth,Yuangao Xu,Rongjun Chen,Song Rong,Nelli Shushakova,Faikah Gueler,Hermann Haller,Florian P Limbourg
CD115, the receptor for colony stimulating factor 1 (CSF-1), is essential for survival and differentiation of monocytes and macrophages and is therefore frequently used to define monocyte subsets and their progenitors in immunological assays. However, CD115 surface expression and detection by flow cytometry is greatly influenced by cell isolation and processing methods, organ source and disease context
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VISTA Non-redundantly Regulates Proliferation and CD69low γδ T cell Accumulation in the Intestine in Murine Sepsis. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-30 Chyna C Gray,Brandon E Armstead,Chun-Shiang Chung,Yaping Chen,Alfred Ayala
Sepsis is a dysregulated systemic immune response to infection that is responsible for ∼35% of in-hospital deaths at a significant fiscal health care cost. Our laboratory, among others, has demonstrated the efficacy of targeting negative checkpoint regulators (NCRs) to improve survival in a murine model of sepsis, cecal ligation and puncture (CLP). B7-CD28 superfamily member, V-domain Immunoglobulin
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LPS and type I&II interferons have opposing effects on epigenetic regulation of LAIR1 expression in mouse and human macrophages. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-27 Hannah K Dorando,Evan C Mutic,Joanna Y Li,Ezri P Perrin,Mellisa K Wurtz,Chaz C Quinn,Jacqueline E Payton
Inhibitory immune receptors are important for maintaining immune homeostasis. We identified epigenetic alterations in two members of this group, LAIR1 and LAIR2, in lymphoma patients with inflammatory tissue damage and susceptibility to infection. We predicted that the expression of LAIR genes is controlled by immune mediators acting on transcriptional regulatory elements. Using flow cytometry, qRT-PCR
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The invasive margin of early-stage human colon tumors is infiltrated with neutrophils of an antitumoral phenotype. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-24 Eduardo Vadillo,Alejandra Mantilla,Cristina Aguilar-Flores,Saraí Gisel De León-Rodríguez,Sandra Vela-Patiño,Juan Badillo,Keiko Taniguchi-Ponciano,Daniel Marrero-Rodríguez,Lourdes Ramírez,Iliana Itzel León-Vega,Carmen Fuentes-Castañeda,Patricia Piña-Sánchez,Jessica Lakshmi Prieto-Chávez,Vadim Pérez-Kondelkova,Juan José Montesinos,Laura Bonifaz,Rosana Pelayo,Héctor Mayani,Michael Schnoor
Neutrophils infiltrate several types of cancer; however, whether their presence is associated with disease progression remains controversial. Here, we show that colon tumors overexpress neutrophil chemoattractants compared to healthy tissues, leading to their recruitment to the invasive margin and the central part of colon tumors. Of note, tumor-associated neutrophils expressing tumor necrosis factor
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High levels of short chain fatty acids secreted by Candida albicans hyphae induce neutrophil chemotaxis via Free Fatty Acid Receptor 2. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-22 Arsham Khamzeh,Agnes Dahlstrand Rudin,Vignesh Venkatakrishnan,Marios Stylianou,Felix P Sanchez Klose,Constantin F Urban,Halla Björnsdottir,Johan Bylund,Karin Christenson
Candida albicans belongs to our commensal mucosal flora and in immune-competent individuals in the absence of epithelial damage, this fungus is well tolerated and controlled by our immune defense. However, C. albicans is an opportunistic microorganism that can cause different forms of infections, ranging from superficial to life-threatening systemic infections. C. albicans is polymorphic and switch
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Temporal and cellular analysis of granuloma development in mycobacterial infected adult zebrafish. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-20 Geyang Luo,Dong Zeng,Jianxin Liu,Duoduo Li,Howard E Takiff,Shu Song,Qian Gao,Bo Yan
Because granulomas are a hallmark of tuberculosis (TB) pathogenesis, the study of the dynamic changes in their cellular composition and morphological character can facilitate our understanding of TB pathogenicity. Adult zebrafish infected with Mycobacterium marinum (M. m) form granulomas that are similar to the granulomas in human TB patients and therefore have been used to study host-mycobacterium
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TIPE proteins control directed migration of human T cells by directing GPCR and lipid second messenger signaling. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-11 Jiyeon Yu,Ali Zamani,Jason R Goldsmith,Zienab Etwebi,Chin Nien Lee,Youhai H Chen,Honghong Sun
Tissue infiltration by circulating leukocytes via directed migration (also referred to as chemotaxis) is a common pathogenic mechanism of inflammatory diseases. G-protein coupled receptors (GPCRs) are essential for sensing chemokine gradients and directing the movement of leukocytes during immune responses. The TNF-α-induced protein 8-like (TIPE or TNFAIP8L) family of proteins are newly described pilot
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Role of Immune Cells in the Pathogenesis of Myocarditis. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-09 Keyu Liu,Bo Han
Myocarditis is an inflammatory heart disease that mostly affects young people. Myocarditis involves a complex immune network; however, its detailed pathogenesis is currently unclear. The diversity and plasticity of immune cells, either in the peripheral blood or in the heart, have been partially revealed in a number of previous studies involving patients and several kinds of animal models with myocarditis
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NKG2D receptor signaling shapes T cell thymic education. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-09 Cynthia Perez,Lourdes Plaza-Rojas,Justin C Boucher,Mate Z Nagy,Elena Kostenko,Kushal Prajapati,Brianna Burke,Michael Delos Reyes,Anna L Austin,Shubin Zhang,Phong T Le,José A Guevara-Patino
The role of natural killer group 2D (NKG2D) in peripheral T cells as a costimulatory receptor is well established. However, its contribution to T cell thymic education and functional imprint is unknown. Here, we report significant changes in development, receptor signaling, transcriptional program, and function in T cells from mice lacking NKG2D signaling. In C57BL/6 (B6) and OT-I mice, we found that
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Adoptive cell immunotherapy for breast cancer: harnessing the power of immune cells. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-09 Xue Li,Yunan Zhu,Jinfeng Yi,Yuhan Deng,Bo Lei,He Ren
Breast cancer is the most prevalent malignant neoplasm worldwide, necessitating the development of novel therapeutic strategies owing to the limitations posed by conventional treatment modalities. Immunotherapy is an innovative approach that has demonstrated significant efficacy in modulating a patient's innate immune system to combat tumor cells. In the era of precision medicine, adoptive immunotherapy
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Inulin prebiotic ameliorates type 1 diabetes dictating regulatory T cell homing via CCR4 to pancreatic islets and butyrogenic gut microbiota in murine model. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-08 Jhefferson Barbosa Guimarães,Vanessa Fernandes Rodrigues,Ítalo Sousa Pereira,Gabriel Martins da Costa Manso,Jefferson Elias-Oliveira,Jefferson Antônio Leite,Mariana Camila Gonçalves Miranda Waldetario,Sarah de Oliveira,Arilson Bernardo Dos Santos Pereira Gomes,Ana Maria Caetano Faria,Simone Gusmão Ramos,Vânia L D Bonato,João Santana Silva,Marco Aurélio Ramirez Vinolo,Ulliana Marques Sampaio,Maria Teresa
Gut dysbiosis is linked to Type 1 diabetes mellitus (T1D). Inulin, a prebiotic, modulates the gut microbiota, promoting beneficial bacteria that produce essential short-chain fatty acids for immune regulation. However, how inulin affects T1D remains uncertain. Using a streptozotocin-induced (STZ) mouse model, we studied inulin's protective effects. Remarkably, STZ+INU mice resisted T1D, with none developing
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Construction of truncated PSMA as a PET reporter gene for CAR-T cell trafficking. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-07 Yirui Zhang,Xiangming Song,Zhuoshuo Xu,Xiaoying Lv,Yu Long,Xiaoli Lan,Ping Lei
In solid tumors, there are multiple barriers for a CAR T cell to surmount in order to reach the tumor site. For better understanding whether CAR T cells effectively infiltrate into tumor site and simultaneously, whether there are off-target effects, real-time monitoring technologies need to be established. Cell-based Positron Emission Tomography (PET) reporter genes have been developed to monitor engineered
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Follicular helper T cell: Emerging roles in lymphomagenesis. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-06 Ji-Nuo Wang,Gaofeng Zheng,Wenjun Wu,He Huang
Follicular helper T cells (Tfh) are a subset of CD4+ T cells that are fundamental to forming germinal centers, which are the primary sites of antibody affinity maturation and the proliferation of activated B cells. Tfh cells have been extensively studied over the past 10 years, especially regarding their roles in cancer genesis. This review describes the characteristics of normal Tfh cells and focuses
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Unveiling the immune system ageing in single-cell resolution. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-02 Chun Lai Chan,Ryohichi Sugimura
This writing serves as a commentary on the findings presented in the original manuscript by Yang et al. in 2023, published in the Journal of Leukocyte Biology (JLB). This commentary first summarizes the spatial-temporal dynamics of regulatory T-cells (T-reg) derived from mice (Tabula Muris Senis) of different ages (3M, 18M, and 24M) at different anatomical niches like lymph nodes and bone marrow. We
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Single-cell transcriptomics reveals tumor-infiltrating B cell function after neoadjuvant pembrolizumab and chemotherapy in non-small cell lung cancer. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-01 Lingjie Hou,Siyuan Zhang,Wenwen Yu,Xuena Yang,Meng Shen,Xishan Hao,Xiubao Ren,Qian Sun
Non-small cell lung cancer (NSCLC) is the most pervasive lung cancer subtype. Recent studies have shown that immune checkpoint inhibitors achieved favorable clinical benefits in resectable NSCLC; however, the associated mechanism remains unclear. The role of T cells in antitumor immunity has received considerable attention, while the antitumor effects of tumor-infiltrating B cells (TIBs) in NSCLC remain
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Neutrophils sing "IL[-10] be seeing you" in the lungs during pneumonia. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-01 Matthew K McPeek,John C Gomez,Claire M Doerschuk
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TLR7 activation of age-associated B cells mediates disease in a mouse model of primary Sjögren's disease. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-10-31 Achamaporn Punnanitinont,Eileen M Kasperek,Chengsong Zhu,Guan Yu,Jeffrey C Miecznikowski,Jill M Kramer
Primary Sjögren's disease (pSD, also referred to as Sjögren's syndrome) is an autoimmune disease that primarily occurs in women. In addition to exocrine gland dysfunction, pSD patients exhibit B cell hyperactivity. B cell-intrinsic TLR7 activation is integral to the pathogenesis of SLE, a disease that shares similarities with pSD. The role of TLR7-mediated B cell activation in pSD, however, remains
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Phenotypic alteration by dengue virus serotype-2 delays neutrophil apoptosis and stimulates the release of prosurvival secretome with immunomodulatory functions. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-10-27 Surender Rawat,Shubham Kumar,Shweta Duggal,Arup Banerjee
Neutrophils are the most abundant, phenotypically heterogeneous, and exert detrimental or protective roles during anti-viral response. Dengue virus has been reported to activate neutrophils. However, the effect of the dengue virus on the neutrophil phenotypes, survival, and release of inflammatory secretome is yet to be understood. Herein, we investigated the effect of dengue virus serotype-2 (DV-2)
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Correction to: T cell functions and organ infiltration by leukemic T cells require cortactin. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-10-26
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Extracellular vesicles: secret agents of inflammation and the importance of their identification. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-10-26 Robert Maile
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A new hope for early T cell precursor acute lymphoblastic leukemia therapy based on STAT5+ leukemic stem cell targeting. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-10-26 Rosana Pelayo
Leukemia stem cells are known to drive tumor progression, drug resistance, microenvironmental shift, and relapse, which would make them a perfect therapeutic target. However, their phenotypic and functional similarity to their normal counterparts leaves limited road maps for their selective elimination. Tremblay et al. recently unraveled the fundamental role of overactivated pSTAT5 as a functional
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Cytomegalovirus durably primes neutrophil oxidative burst. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-10-26 Thomas F Marandu,Michael Dombek,Michael Gutknecht,Marion Griessl,Ingred Goretti Riça,Barbora Vlková,Kristína Macáková,Eleni Panagioti,Alec Griffith,James Lederer,Michael Yaffe,Sidharth Shankar,Leo Otterbein,Kiyoshi Itagaki,Carl J Hauser,Charles H Cook
Cytomegalovirus (CMV) is a ubiquitous herpes virus that infects most humans, thereafter persisting lifelong in tissues of the host. It is a known pathogen in immunosuppressed patients, but its impact on immunocompetent hosts remains less understood. Recent data have shown that CMV leaves a significant and long-lasting imprint in host immunity that may confer some protection against subsequent bacterial
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Recombinant GM-CSF enhances the bactericidal ability of PMNs by increasing intracellular IL-1β and improves the prognosis of secondary Pseudomonas aeruginosa pneumonia in sepsis. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-10-26 Fuquan Tu,Lili Pan,Wenwei Wu,Yuanhua Cai,Jinggang Li,Xuechun Wang,Xiaolin Lai,Zhixiang Chen,Luya Ye,Shaoyuan Wang
This study tested the hypothesis that recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) enhances polymorphonuclear neutrophils (PMNs) via interleukin (IL)-1β to improve the prognosis of secondary infection in sepsis. The latter stage of sepsis is prone to induce immunosuppression, resulting in secondary fatal infections. Recombinant GM-CSF has become a way for sepsis-induced immunosuppression
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Food-sensitized pediatric patients show colonic cow's milk protein-specific Th2 cells. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-10-26 Karina E Canziani,María Carolina Ruiz,Martín R Candia,Manuela Ilid,Emanuel Feregotti,Renata Curciarello,María Cecilia Álvarez,Luciana Guzmán,Viviana Bernedo,Marcela García,Barbara Bohle,Guillermo Horacio Docena,Cecilia Isabel Muglia
Food allergies have become a health concern worldwide. Around 6% to 10% of children are allergic to cow's milk proteins. We have previously characterized colorectal polyps in patients sensitized to food allergens. These polyps are classified as inflammatory and present a type 2 environment, with elevated interleukin (IL)-13 and IL-4, and are a site of immunoglobulin E synthesis. In this study, we characterized
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Amoxicillin treatment of pneumococcal pneumonia impacts bone marrow neutrophil maturation and function. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-10-14 Mélanie Mondemé,Yasmine Zeroual,Daphnée Soulard,Benjamin Hennart,Delphine Beury,Jean-Michel Saliou,Christophe Carnoy,Jean-Claude Sirard,Christelle Faveeuw
Pneumonia caused by Streptococcus pneumoniae is a leading cause of death worldwide. A growing body of evidence indicates that the successful treatment of bacterial infections results from synergy between antibiotic-mediated direct antibacterial activity and the host's immune defenses. However, the mechanisms underlying the protective immune responses induced by amoxicillin, a β-lactam antibiotic used
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Dysfunctional States of Unconventional T cell Subsets in Cancer. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-10-14 Elizabeth N Katsnelson,Andrea Spengler,Joanne Domenico,Kasey L Couts,Liyen Loh,Laurent Gapin,Martin D McCarter,Richard P Tobin
Unconventional T cells (UCTs) represent a promising therapeutic agent to overcome the current limitations of immunotherapies due to their universal T-cell receptors (TCRs), ability to respond directly to cytokine stimulation, and capacity to recruit and modulate conventional immune cells in the tumor microenvironment (TME). Like conventional T-cells, UCTs can enter a dysfunctional state and the functional
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G-CSF reshapes the cytosolic PCNA scaffold and modulates glycolysis in neutrophils. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-10-12 Karen Aymonnier,Enzo Bosetta,Nathan G F Leborgne,Audrey Ullmer,Morgane Le Gall,Alessia De Chiara,Virginie Salnot,Souganya Mani,Patrizia Scapini,Ian Wicks,Simon Chatfield,Katherine R Martin,Veronique Witko-Sarsat
Cytosolic proliferating cell nuclear antigen (PCNA) is involved in neutrophil survival and function, where it acts as a scaffold and associates with proteins involved in apoptosis, NADPH oxidase activation, cytoskeletal dynamics and metabolism. While the PCNA interactome has been characterized in neutrophils under homeostatic conditions, less is known about neutrophil PCNA in pathophysiological contexts
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Effects of lysine deacetylase inhibitor treatment on LPS responses of alveolar-like macrophages. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-10-09 Sara Russo,Marcel Kwiatkowski,Justina C Wolters,Albert Gerding,Jos Hermans,Natalia Govorukhina,Rainer Bischoff,Barbro N Melgert
Macrophages are key immune cells that can adapt their metabolic phenotype in response to different stimuli. Lysine deacetylases (KDAC) are important enzymes regulating inflammatory gene expression and KDAC inhibitors have been shown to exert anti-inflammatory effects in models of chronic obstructive pulmonary disease (COPD). We hypothesized that these anti-inflammatory effects may be associated with
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Bacteria- and Fungus-derived PAMPs Induce Innate Immune Memory via Similar Functional, Metabolic and Transcriptional Adaptations. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-10-04 Margaret A McBride,Cody L Stothers,Benjamin A Fensterheim,Katherine R Caja,Allison M Owen,Antonio Hernandez,Julia K Bohannon,Naeem K Patil,Sabah Ali,Sujata Dalal,Mohsin Rahim,Irina A Trenary,Jamey D Young,David L Williams,Edward R Sherwood
Exposure to pathogen-associated molecular patterns (PAMPs) induces an augmented, broad-spectrum antimicrobial response to subsequent infection, a phenomenon termed innate immune memory. This study examined the effects of treatment with β-glucan, a fungus-derived Dectin-1 ligand, or monophosphoryl lipid A (MPLA), a bacteria-derived TLR4 ligand, on innate immune memory with a focus on identifying common
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The effect of leptin on trained innate immunity and on systemic inflammation in subjects with obesity. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-09-30 Daniela Flores Gomez,Siroon Bekkering,Rob Ter Horst,Benjamin Cossins,Inge C L van den Munckhof,Joost H W Rutten,Leo A B Joosten,Mihai G Netea,Niels P Riksen
Leptin is associated with cardiometabolic complications of obesity, such as metabolic syndrome and atherosclerosis. In obese men, the presence of metabolic syndrome is associated with higher circulating leptin and interleukin-6 concentrations, and increased monocyte cytokine production capacity. Here we investigated the effects of leptin on monocyte function and systemic inflammatory markers in obese
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A novel opsonic eCIRP inhibitor for lethal sepsis. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-09-30 Colleen P Nofi,Chuyi Tan,Gaifeng Ma,Molly Kobritz,Jose Prince,Haichao Wang,Monowar Aziz,Ping Wang
Sepsis is a life-threatening inflammatory condition partly orchestrated by the release of various damage-associated molecular patterns (DAMPs) such as extracellular cold-inducible RNA-binding protein (eCIRP). Despite advances in understanding the pathogenic role of eCIRP in inflammatory diseases, novel therapeutic strategies to prevent its excessive inflammatory response are lacking. Milk fat globule-epidermal
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Lysosomal control of dendritic cell function. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-24 Jia Yang,Shan-Jie Rong,Hai-Feng Zhou,Chao Yang,Fei Sun,Jun-Yi Li
Lysosomal compartments undergo extensive remodeling during dendritic cell (DC) activation to meet the dynamic functional requirements of DCs. Instead of being regarded as stationary and digestive organelles, recent studies have increasingly appreciated the versatile roles of lysosomes in regulating key aspects of DC biology. Lysosomes actively control DC motility by linking calcium efflux to the actomyosin
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GKT137831 in combination with adipose-derived stem cells alleviates high glucose-induced inflamm-aging and improves diabetic wound healing. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-09-29 Yunxian Dong,Youliang Zhang,Fangwei Li,Bing Tang,Dongming Lv,Haibin Wang,Shengkang Luo
Adipose stem cells (ADSCs) have been proven to promote healing in diabetic wounds, which are one of the most serious chronic refractory wounds. However, reactive oxygen species (ROS) induced by high glucose (HG) lead to oxidative stress and aging in ADSCs, which limits the therapeutic effect of ADSCs. In this study, we investigated the role of GKT137831, a NOX1/4 inhibitor that can reduce ROS production
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Inflammatory macrophages exploited by oral streptococcus increase IL-1B release via NLRP6 inflammasome. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-09-27 Sarah Metcalfe,Michelle Panasiewicz,Jason G Kay
Chronic inflammatory periodontal disease develops in part from the infiltration of a large number of classically activated inflammatory macrophages that release inflammatory cytokines important for disease progression, including inflammasome-dependent interleukin (IL)-1β. Streptococcus gordonii is a normally commensal oral microorganism; while not causative, recent evidence indicates that commensal
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HIVEP3 inhibits fate decision of CD8+ invariant NKT cells after positive selection. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-09-27 Qielan Wu,Shiyu Bai,Miya Su,Yuwei Zhang,Xuran Chen,Ting Yue,Linfeng Xu,Lu Wang,Di Xie,Shuhang Li,Xiang Li,Sicheng Fu,Lili Wang,Chenxi Tian,Jun Pan,Yuanyuan Huang,Yuting Cai,Yu Wang,Fang Hu,Fengyin Li,Huimin Zhang,Li Bai
CD8+ invariant natural killer T (iNKT) cells are functionally different from other iNKT cells and are enriched in human but not in mouse. To date, their developmental pathway and molecular basis for fate decision remain unclear. Here, we report enrichment of CD8+ iNKT cells in neonatal mice due to their more rapid maturation kinetics than CD8- iNKT cells. Along developmental trajectories, CD8+ and
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Human CXCR1 knock-in mice infer functional expression of a murine ortholog. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-09-27 Farnaz Fahimi,Md Jahangir Alam,Caroline Ang,Galih Prakasa Adhyatma,Liang Xie,Charles R Mackay,Remy Robert
Targeting CXCR1 and CXCR2 chemokine receptors to block neutrophil migration to sites of inflammation is a promising therapeutic approach for various inflammatory and autoimmune diseases. However, assessing the translational potential of such therapies using mouse models is challenging due to the unclear expression of CXCR1 at the protein level. Although CXCR2 has been well characterized in both mice
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SARS-CoV-2 mRNA vaccine-induced immune responses in rheumatoid arthritis. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-09-27 Marc-André Limoges,Audrey Lortie,Élodie Demontier,Akouavi Julite Irmine Quenum,Félix Lessard,Zacharie Drouin,Nathalie Carrier,Leopold Mbous Nguimbus,Marie-Claude Beaulieu,Gilles Boire,Alain Piché,Hugues Allard-Chamard,Sheela Ramanathan,Sophie Roux
Our objective was to characterize T and B cell responses to vaccination with SARS-CoV-2 antigens in immunocompromised rheumatoid arthritis (RA) patients. In 22 RA patients, clinical and biological variables were analyzed before and 4 weeks after each of 3 messenger RNA (mRNA) vaccine doses and compared with unmatched healthy individuals. Sequentially sampled peripheral blood mononuclear cells and sera
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Coculturing Liver Cancer Cells and Monocytes in Spheroids Conditions Monocytes to Adopt Tumor-associated Macrophage Phenotypes that Favor Tumor Growth via Cholesterol Metabolism. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-09-23 Pornlapat Keawvilai,Patipark Kueanjinda,Jeerameth Klomsing,Tanapat Palaga
Tumor-infiltrating immune cells and their crosstalk with cancer cells in the tumor microenvironment (TME) play a crucial role in shaping tumor progression and response to therapy. We utilized three-dimension (3D) liver cancer spheroids incorporating human primary monocytes to investigate the crosstalk between tumor-associated macrophages (TAMs) and Hepatocellular carcinoma (HCC) cells, HepG2 and PLC/PRF5
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NKG2A discriminates natural killer cells with a suppressed phenotype in pediatric acute leukemia. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-09-21 Aina Ulvmoen,Victor Greiff,Anne G Bechensteen,Marit Inngjerdingen
NK cells are important for early tumor immune surveillance. In patients with hematological cancers, NK cells are generally functional deficient and display dysregulations in their receptor repertoires. Acute leukemia is the most common cancer in children, and we here performed a comparative phenotypic profiling of NK cells from pre-B cell acute lymphoblastic leukemia (BCP-ALL) patients to identify
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Macrophage-expressed SRA ameliorates alcohol-induced liver injury by suppressing S-glutathionylation of Notch1 via recruiting Thioredoxin. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-09-19 Lei Li,Jialiang Luo,Zhengyumeng Zhu,Ping Wang,Qishan Xu,Bo Chang,Di Wang,Lu Yu,Xiao Lu,Jia Zhou,Qingyun Chen,Daming Zuo
Scavenger receptor A (SRA) is preferentially expressed in macrophages and implicated as a multifunctional pattern recognition receptor for innate immunity. Hepatic macrophages play a primary role in the pathogenesis of alcoholic liver disease (ALD). Herein, we observed that SRA expression was significantly increased in the liver tissues of mice with alcohol-related liver injury. SRA-deficient (SRA-/-)
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Immune cells in skin inflammation, wound healing and skin cancer. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-09-18 Yuhan Zhang,Qianjin Lu
Given the self-evident importance of cutaneous immunity in the maintenance of body-surface homeostasis, disturbance of the steady-state skin is inextricably intertwined with dysfunction in cutaneous immunity. It is often overlooked by people that skin, well-known as a solid physical barrier, is also a strong immunological barrier considering the abundant presence of immune cells including lymphocytes
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Adjuvant activities of immunostimulating natural products: Astragalus membranaceus (Fisch.) Bge and Coriolus versicolor in BNT162b2 vaccination against COVID-19 infection. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-09-15 Ben Chung-Lap Chan,Peiting Li,Miranda Sin-Man Tsang,Johnny Chun-Chau Sung,Keith Wai-Yeung Kwong,Tao Zheng,Sharon Sze-Man Hon,Ching-Po Lau,Ronald Chi-Yan Ho,Fang Chen,Clara Bik-San Lau,Ping-Chung Leung,Chun-Kwok Wong
The global pandemic of coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been developing all over the world for more than three years. In late 2020, several variants of concern (VOC) of SARS-CoV-2 virus emerged, with increased viral fitness and transmissibility by mutations of the spike proteins of the viral particle, denting hopes that
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From lipid nanoparticles to precision therapies for hematologic diseases: getting closer. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-24 Hector Mayani
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Single-cell transcriptomics of Treg reveals hallmarks and trajectories of immunological aging. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-09-07 Kevin Y Yang,Jinyue Liao,Zhangjing Ma,Hung Fat Tse,Liwei Lu,Luis Graca,Kathy O Lui
Age-related immunosenescense is characterized by progressive dysfunction of adaptive immune response and increased autoimmunity. Nevertheless, the impact of aging on CD4+ regulatory T cells (Treg) that are master regulators of the immune system remains largely unclear. Here, we report cellular and molecular hallmarks of Treg derived from murine lymphoid and adipose tissues at 3, 18 and 24 months of
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BCG vaccination induces innate immune memory in gamma delta T cells in humans. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-09-06 Tsz K Suen,Simone J C F M Moorlag,Wenchao Li,Charlotte L J de Bree,Valerie A C M Koeken,Vera P Mourits,Helga Dijkstra,Heidi Lemmers,Jaydeep Bhat,Cheng-Jian Xu,Leo A B Joosten,Joachim L Schultze,Yang Li,Katarzyna Placek,Mihai G Netea
Bacillus Calmette-Guérin (BCG) vaccine is well-known for inducing trained immunity in myeloid and natural killer cells, which can explain its cross-protective effect against heterologous infections. Although displaying functional characteristics of both adaptive and innate immunity, γδ T cell memory has been only addressed in a pathogen-specific context. In this study we aimed to determine whether
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Mitochondria-induced formation of neutrophil extracellular traps is enhanced in the elderly via Toll-like receptor 9. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-24 Michal Pastorek,Barbora Konečná,Jakub Janko,Ľubica Janovičová,Ľudmila Podracká,Jozef Záhumenský,Emöke Šteňová,Martin Dúbrava,Július Hodosy,Barbora Vlková,Peter Celec
Neutrophil extracellular traps are potent antimicrobial weapons; however, their formation during sterile inflammation is detrimental, and the mechanism of induction is still unclear. Since advanced age is the primary clinical risk factor for poor outcomes in inflammatory diseases, we hypothesized that sterile stimuli, represented by mitochondria, would induce neutrophil extracellular trap formation
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Dichotomous roles of RIPK3 in regulating the IFN response and NLRP3 inflammasome in human monocytes. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-24 Chao Yang,Ruoxi Yuan,Caroline Brauner,Yong Du,Marie Dominique Ah Kioon,Franck J Barrat,Lionel B Ivashkiv
Regulation of the profile and magnitude of toll-like receptor (TLR) responses is important for effective host defense against infections while minimizing inflammatory toxicity. The chemokine CXCL4 regulates the TLR8 response to amplify inflammatory gene and inflammasome activation while attenuating the interferon (IFN) response in primary monocytes. In this study, we describe an unexpected role for
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Dengue Virus Infection In Mice Induces Bone Marrow Myeloid Cell Differentiation And Generates Ly6glow Immature Neutrophils With Modulated Functions. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-08-30 Shweta Duggal,Surender Rawat,Gazala Siddqui,Preeti Vishwakarma,Sweety Samal,Arup Banerjee,Sudhanshu Vrati
While neutrophil activation during dengue virus (DV) infection is known, the effect of DV infection on neutrophil biogenesis has not been studied. We demonstrate that DV serotype-2 (DV2) induces the differentiation of mice progenitor cells ex vivo towards the CD11b + Ly6C + Ly6G+ granulocyte population. We further observed an expansion of CD11b + Ly6CintLy6Glow myeloid cells in the bone marrow of DV2-infected
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Macrophage extracellular traps promote tumor-like biologic behaviors of fibroblast-like synoviocytes through cGAS-mediated PI3K/Akt signaling pathway in patients with rheumatoid arthritis. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-08-30 Weizhen Weng,Yan Liu,Zuoyu Hu,Zhihui Li,Xiaohua Peng,Manli Wang,Bo Dong,Shuyuan Zhong,Yutong Jiang,Yunfeng Pan
Rheumatoid arthritis (RA) is an autoimmune disease characterized by synovium hyperplasia and bone destruction. Macrophage extracellular traps (METs) are released from macrophages under various stimulus and may generate stable autoantigen-DNA complexes, aggravate autoantibodies generation and autoimmune responses. We aimed to investigate the role of METs on the biologic behaviors of RA-FLSs. Synovial
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Chemokine Cxcl1-Cxcl2 heterodimer is a potent neutrophil chemoattractant. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-24 Kirti V Sawant,Krishna Mohan Sepuru,Brigith Penaranda,Emily Lowry,Roberto P Garofalo,Krishna Rajarathnam
Microbial infection is characterized by release of multiple proinflammatory chemokines that direct neutrophils to the insult site. How collective function of these chemokines orchestrates neutrophil recruitment is not known. Here, we characterized the role for heterodimer and show that the Cxcl1-Cxcl2 heterodimer is a potent neutrophil chemoattractant in mice and can recruit more neutrophils than the
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Organoids in COVID-19: Can we break the glass ceiling? J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-08-24 Chau Chiu Wang,Ryohichi Sugimura
COVID-19 emerged in Sep 2020 as a disease caused by the virus SARS-CoV-2. The disease presented as pneumonia at first, but later was shown to cause multi-system infections and long-term complications. Many efforts have been put into discovering the exact pathogenesis of the disease. In this review, we aim to discuss an emerging tool in disease modelling, organoids in the investigation of COVID-19.
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A Stitch of "CD9" saves Nine: CD9+ Tissue Resident Memory T cells in Sjogren's Syndrome and Autoimmunity. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-08-17 Dan Li,Song Zhang
This editorial underscores the role of CD9+ Tissue Resident Memory (Trm) cells in autoimmune diseases, specifically in Sjogren's Syndrome, and points to potential implications for better understanding the nature of recurrent autoimmune flares. New findings from Chang et al., discussed in the piece, highlight the presence and functional role of CD8+Trm in inflamed labial glands in Sjogren's Syndrome
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ILC2 influence the differentiation of alveolar type II epithelial cells in bronchopulmonary dysplasia mice. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-24 Hong-Yan Lu,Ming-Yan Wang,Shao-Xuan Zhu,Hui-Min Ju,Su-Qing Xu,Yu Qiao,Shan-Jie Wei,Zhao-Liang Su
Bronchopulmonary dysplasia, a common complication of premature infants, is mainly characterized by blocked alveolarization. Proverbially, the injury of alveolar type II epithelial cells is regarded as the pathologic basis of occurrence and development of bronchopulmonary dysplasia. In the case of alveolar epithelial damage, alveolar type II epithelial cells can also differentiate to alveolar type I
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Proliferation of monocytes and macrophages in homeostasis, infection, injury, and disease. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-24 Jingbo Pang,Timothy J Koh
Monocytes (Mo) and macrophages (Mφ) play important roles in the function of tissues, organs, and systems of all animals during homeostasis, infection, injury, and disease. For decades, conventional wisdom has dictated that Mo and Mφ are end-stage cells that do not proliferate and that Mφ accumulation in tissues is the result of infiltration of Mo from the blood and subsequent differentiation to Mφ
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Nonfunctional TGF-β/ALK1/ENG signaling pathway supports neutrophil proangiogenic activity in hereditary hemorrhagic telangiectasia. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-24 Inga Duerig,Ekaterina Pylaeva,Irem Ozel,Sami Wainwright,Ilona Thiel,Sharareh Bordbari,Maksim Domnich,Elena Siakaeva,Antonia Lakomek,Felicia Toppe,Carolin Schleupner,Urban Geisthoff,Stephan Lang,Freya Droege,Jadwiga Jablonska
The transforming growth factor β (TGF-β)/ALK1/ENG signaling pathway maintains quiescent state of endothelial cells, but at the same time, it regulates neutrophil functions. Importantly, mutations of this pathway lead to a rare autosomal disorder called hereditary hemorrhagic telangiectasia (HHT), characterized with abnormal blood vessel formation (angiogenesis). As neutrophils are potent regulators
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The mutual regulation between γδ T cells and macrophages during wound healing. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-07-26 Wengang Hu,Xiaorong Zhang,Hao Sheng,Zhongyang Liu,Yunxia Chen,Yong Huang,Weifeng He,Gaoxing Luo
Macrophages are the main cells shaping the local microenvironment during wound healing. As the prime T cells in the skin, γδ T cells participate in regulating microenvironment construction, determining their mutual regulation helps to understand the mechanisms of wound healing, and explore innovative therapeutic options for wound repair. This review introduced their respective role in wound healing
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Neutrophil nucleus: shaping the past and the future. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-24 Julia Salafranca,Jacky Ka Ko,Ananda K Mukherjee,Marco Fritzsche,Erinke van Grinsven,Irina A Udalova
Neutrophils are innate immune cells that are key to protecting the host against infection and maintaining body homeostasis. However, if dysregulated, they can contribute to disease, such as in cancer or chronic autoinflammatory disorders. Recent studies have highlighted the heterogeneity in the neutrophil compartment and identified the presence of immature neutrophils and their precursors in these
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Lipid Metabolism in the Immune Niche of Tumor-Prone Liver Microenvironment. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-07-20 Jintian Chen,Thomas T H Chan,Jingying Zhou
The liver is not only a common primary site for tumorigenesis, but also cancer metastasis. Advanced cancer patients with liver metastases also show reduced response rates and survival benefits when treated with immune checkpoint inhibitors. Accumulating evidence has highlighted the importance of the liver immune microenvironment in determining tumorigenesis, metastasis-organotropism as well as immunotherapy
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Human neutrophil Fc gamma receptors: different buttons for different responses. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-24 Omar Rafael Alemán,Carlos Rosales
Neutrophils are fundamental cells in host defense. These leukocytes are quickly recruited from the blood to sites of infection or tissue damage. At these sites, neutrophils initiate several innate immune responses, including phagocytosis, production of reactive oxygen species, degranulation to release proteases and other antimicrobial compounds, production of inflammatory mediators, and formation of
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Human γδ T cell identification from single-cell RNA sequencing datasets by modular TCR expression. J. Leukoc. Biol. (IF 5.5) Pub Date : 2023-11-24 Zheng Song,Lara Henze,Christian Casar,Dorothee Schwinge,Christoph Schramm,Johannes Fuss,Likai Tan,Immo Prinz
Accurately identifying γδ T cells in large single-cell RNA sequencing (scRNA-seq) datasets without additional single-cell γδ T cell receptor sequencing (sc-γδTCR-seq) or CITE-seq (cellular indexing of transcriptomes and epitopes sequencing) data remains challenging. In this study, we developed a TCR module scoring strategy for human γδ T cell identification (i.e. based on modular gene expression of