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Regulatory roles of ACSL5 in the anti-tumor function of palmitic acid (C16:0) via the ERK signaling pathway. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-11-08 Jiapei Lv,Wang Yanting,Shan Wei
Previous studies have highlighted the susceptibility of cancer to perturbations in lipid metabolism. In particular, C16:0 has emerged as a promising novel treatment for hepatocellular carcinoma. In our study, we investigated the levels of C16:0 in the serum of non-small lung cancer patients were significant downregulation compared to healthy individuals (n=10; p<0.05). Moreover, our in vitro experiments
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Expression and role of cocaine-amphetamine regulated transcript (CART) in the proliferation of biliary epithelium. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-10-19 Arianna Casini,Giorgio Vivacqua,Rosa Vaccaro,Anastasia Renzi,Stefano Leone,Luigi Pannarale,Antonio Franchitto,Paolo Onori,Romina Mancinelli,Eugenio Gaudio
Cholangiocytes, the epithelial cells that line the biliary tree, can proliferate under the stimulation of several factors through both autocrine and paracrine pathways. The cocaine-amphetamine-regulated-transcript (CART) peptide has several physiological functions, and it is widely expressed in several organs. CART increases the survival of hippocampal neurons by upregulating brain-derived neurotrophic
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Ozone and procaine increase secretion of platelet-derived factors in platelet-rich plasma. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-10-10 Chiara Rita Inguscio,Barbara Cisterna,Maria Assunta Lacavalla,Franco Donati,Osvaldo Angelini,Gabriele Tabaracci,Manuela Malatesta
Platelet-rich plasma (PRP) is gaining more and more attention in regenerative medicine as an innovative and efficient therapeutic approach. The regenerative properties of PRP rely on the numerous bioactive molecules released by the platelets: growth factors are involved in proliferation and differentiation of endothelial cells and fibroblasts, angiogenesis and extracellular matrix formation, while
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Seasonal patterns of prolactin, prolactin receptor, and STAT5 expression in the ovaries of wild ground squirrels (Citellus dauricus Brandt). Eur. J. Histochem. (IF 2.0) Pub Date : 2023-10-02 Qingjing Gao,Wenqian Xie,Wenjing Lu,Yuning Liu,Haolin Zhang,Yingying Han,Qiang Weng
Prolactin (PRL) is a hormone crucial for normal reproduction, functioning as an autocrine, paracrine, and endocrine factor. This study aimed to examine the immunolocalization and expression patterns of PRL, prolactin receptor (PRLR), and signal transducer and activator of transcription 5 (STAT5) in the ovaries of wild ground squirrels during both breeding and non-breeding periods. Significant seasonal
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Management of autofluorescence in formaldehyde-fixed myocardium: choosing the right treatment. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-10-02 Zhao Zhang,Hongming Fan,William Richardson,Bruce Z Gao,Tong Ye
Autofluorescence (AF) poses challenges for detecting proteins of interest in situ when employing immunofluorescence (IF) microscopy. This interference is particularly pronounced in strongly autofluorescent tissues such as myocardium, where tissue AF can be comparable to IF. Although various histochemical methods have been developed to achieve effective AF suppression in different types of tissue, their
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Crocin exerts anti-tumor effect in colon cancer cells via repressing the JAK pathway. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-09-12 Hui Yang,Yunlong Zhang,Desheng Zhang,Liping Qian,Tianxing Yang,Xiaocheng Wu
Crocin has been reported to have therapeutic effects on multiple cancers including colon cancer, but its specific mechanism is still ambiguous and needs to be further explored. Human colorectal adenocarcinoma cells (HCT-116) and human normal colonic epithelial cells (CCD841) were first treated with increasing concentrations of crocin. Subsequently, with 150 and 200 μM of crocin, the cell vitality was
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Pretreatment with geniposide mitigates myocardial ischemia/reperfusion injury by modulating inflammatory response through TLR4/NF-κB pathway. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-09-08 Yanmei Yao,Leqing Lin,Wenxue Tang,Yueliang Shen,Fayu Chen,Ning Li,Baiyong Wang
Geniposide (GEN), a medical herb, is known for its therapeutic applications in cardiovascular diseases, though its efficacy in treating myocardial ischemia/reperfusion injury (MI/RI) is yet to be fully elucidated. This study is an endeavor to explore the potential protective mechanism of GEN against MI/RI. To simulate the MI/RI condition, the left anterior descending artery was occluded for 30 min
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Developmental expression of high-mobility group box 1 (HMGB1) in the mouse cochlea. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-09-01 Wenjing Liu,Shanshan Ming,Xiaobing Zhao,Xin Zhu,Yuxiang Gong
The expression changes of high-mobility group box 1 (HMGB1) in the mouse cochlea have recently been implicated in noise-induced hearing loss, suggesting that HMGB1 participates in regulating cochlear function. However, the precise role of HMGB1 in the auditory system remains largely unclear. This study aimed to investigate its function in the developing mouse cochlea by examining the expression pattern
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Expression and clinical significance of HER2/neu, aromatase P450 and adhesion molecule CD24 in endometrial cancer. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-08-10 Liyun Guan,Ying Wang,Jianxin Cheng,Jun Zhang,Shan Kang
This study aimed at exploring the expression and clinical significance of aromatase P450, adhesion molecule CD24 and HER2/neu in endometrial cancer. The expression of aromatase P450, adhesion molecule CD24 and HER2/neu was detected by immunohistochemistry in 15 cases of endometrial hyperplasia group, 50 cases of endometrial adenocarcinoma and 3 cases of uterine papillary adenocarcinoma, with 15 cases
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Expression and localization of α2A-adrenergic receptor in the rat post-natal developing cochlea. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-08-07 Chaoyong Tian,Yang Yang,Yao Li,Fei Sun,Juan Qu,Dingjun Zha
Lots of adrenergic receptors (ARs) are widely present across the auditory pathways and are positioned to affect auditory and vestibular functions. However, noradrenergic regulation in the cochlea has not been well characterized. In this study, a rat model of noise-induced hearing loss was developed to investigate the expression of α2A-adrenergic receptor (AR) after acoustic trauma, then, we investigated
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Quercetin reverses 5-fluorouracil resistance in colon cancer cells by modulating the NRF2/HO-1 pathway. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-08-07 Zhongzhu Tang,Lei Wang,Yunwang Chen,Xiaomin Zheng,Runyu Wang,Bingxue Liu,Shiqi Zhang,Huimin Wang
Quercetin (Que) has been proven to enhance the chemosensitivity of multiple cancers, including colon cancer (CC). However, whether the combination of Que and 5-fluorouracil (5-FU) has a synergistic effect on drug-resistant CC cells has not previously been reported. The effect of Que (5 and 10 μg/mL) on cell vitality and apoptosis of CC and CC drug-resistant cells was examined using a cell counting
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Tumor cells-derived exosomal PD-L1 promotes the growth and invasion of lung cancer cells in vitro via mediating macrophages M2 polarization. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-08-01 Xiangjun Lu,Jian Shen,Siyuan Huang,Dongdong Liu,Haitao Wang
Lung cancer originating from the bronchial epithelium is the most common lung malignancy. It has been reported that programmed cell death 1 ligand 1 (PD-L1) and tumor-associated macrophages are closely related to the development of lung cancer. However, whether tumor-derived exosomal PD-L1 could mediate the regulation of macrophage polarization in lung cancer remains unclear. For this research, the
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Substance P is overexpressed in cervical squamous cell carcinoma and promoted proliferation and invasion of cervical cancer cells in vitro. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-07-31 Ying Wang,Shifa Yuan,Jing Ma,Hong Liu,Lizhen Huang,Fengzhen Zhang
This study aimed to investigate the expression and function of substance P in cervical squamous cell carcinoma. Cancer tissues and adjacent tissues of 20 patients with cervical squamous cell carcinoma in our hospital were collected. The expression of substance P was detected by immunohistochemistry and Western blot analysis. Cervical squamous cell carcinoma line SiHa was treated with different concentrations
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Therapeutic effects of human umbilical cord mesenchymal stem cell-derived extracellular vesicles on ovarian functions through the PI3K/Akt cascade in mice with premature ovarian failure. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-07-27 Nan Li,Xue Fan,Lihong Liu,Yanbing Liu
Premature ovarian failure (POF) mainly refers to ovarian dysfunction in females younger than forty. Mesenchymal stem cells (MSCs) are considered an increasingly promising therapy for POF. This study intended to uncover the therapeutic effects of human umbilical cord MSC-derived extracellular vesicles (hucMSCEVs) on POF. hucMSCs were identified by observing morphology and examining differentiation capabilities
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Dexmedetomidine attenuates neuroinflammation and microglia activation in LPS-stimulated BV2 microglia cells through targeting circ-Shank3/miR-140-3p/TLR4 axis. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-07-26 Guangbao He,Yibo He,Hongwei Ni,Kai Wang,Yijun Zhu,Yang Bao
It has been shown that dexmedetomidine (Dex) could attenuate postoperative cognitive dysfunction (POCD) via targeting circular RNAs (circRNAs). Circ-Shank3 has been found to be involved in the neuroprotective effects of Dex against POCD. However, the role of circ-Shank3 in POCD remains largely unknown. Reverse transcription quantitative PCR (RT-qPCR) was performed to detect circ-Shank3 and miR-140-3p
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Deletion of osteopontin in non-small cell lung cancer cells affects bone metabolism by regulating miR-34c/Notch1 axis: a clue to bone metastasis. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-07-26 Jing Guo,Chang-Yong Tong,Jian-Guang Shi,Xin-Jian Li,Xue-Qin Chen
Lung cancer is prone to bone metastasis, and osteopontin (OPN) has an important significance in maintaining bone homeostasis. The goal of this study was to explore the impact of OPN level on bone metabolism and the molecular mechanism of inhibiting bone metastasis in non-small cell lung cancer (NSCLC). The expression of OPN in NSCLC was ascertained by Western blot and immunohistochemistry, and the
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LncRNA FBXO18-AS promotes gastric cancer progression by TGF-β1/Smad signaling. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-06-20 Yiming Zhang,Wanqiong Zheng,Liang Zhang,Yechun Gu,Lihe Zhu,Yingpeng Huang
For the digestive system, there exists one common malignant tumor, known as gastric cancer. It is the third most prevalent type of tumor among different tumors worldwide. It has been reported that long noncoding RNAs (lncRNAs), participate in various biological processes of gastric cancer. However, there are still many lncRNAs with unknown functions, and we discovered a novel lncRNA designated as FBXO18-AS
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Sodium hyaluronate promotes proliferation, autophagy, and migration of corneal epithelial cells by downregulating miR-18a in the course of corneal epithelial injury. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-06-15 Yingzhuo Guo,Hua Wang
Corneal epithelium can resist the invasion of external pathogenic factors to protect the eye from external pathogens. Sodium hyaluronate (SH) has been confirmed to promote corneal epithelial wound healing. However, the mechanism by which SH protects against corneal epithelial injury (CEI) is not fully understood. CEI model mice were made by scratching the mouse corneal epithelium, and in vitro model
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LncRNA gadd7 promotes mitochondrial membrane potential decrease and apoptosis of alveolar type II epithelial cells by positively regulating MFN1 in an in vitro model of hyperoxia-induced acute lung injury. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-05-31 Guoyue Liu,Cunzhi Yin,Mingjiang Qian,Xuan Xiao,Hang Wu,Fujian Fu
The mortality and morbidity rates of ovarian cancer (OC) are high, but the underlying mechanisms of OC have not been characterized. In this study, we determined the role of Rho GTPase Activating Protein 30 (ARHGAP30) in OC progression. We measured ARHGAP30 abundance in OC tissue samples and cells using immunohistochemistry (IHC) and RT-qPCR. EdU, transwell, and annexin V/PI apoptosis assays were used
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Metformin sensitises osteosarcoma to chemotherapy via the IGF-1R/miR-610/FEN1 pathway. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-05-17 Suwei Dong,Yanbin Xiao,Ziqiang Zhu,Xiang Ma,Zhuohui Peng,Jianping Kang,Jianqiang Wang,Yunqing Wang,Zhen Li
Metformin can enhance cancer cell chemosensitivity to anticancer drugs. IGF-1R is involved in cancer chemoresistance. The current study aimed to elucidate the role of metformin in osteosarcoma (OS) cell chemosensitivity modulation and identify its underlying mechanism in IGF-1R/miR-610/FEN1 signalling. IGF-1R, miR-610, and FEN1 were aberrantly expressed in OS and participated in apoptosis modulation;
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High glucose inhibits neural differentiation by excessive autophagy via peroxisome proliferator-activated receptor gamma. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-05-11 Yin Pan,Di Qiu,Shu Chen,Xiaoxue Han,Ruiman Li
The high prevalence of prediabetes and diabetes globally has led to the widespread occurrence of severe complications, such as diabetic neuropathy, which is a result of chronic hyperglycemia. Studies have demonstrated that maternal diabetes can lead to neural tube defects by suppressing neurogenesis during neuroepithelium development. While aberrant autophagy has been associated with abnormal neuronal
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Knockdown of ARHGAP30 inhibits ovarian cancer cell proliferation, migration, and invasiveness by suppressing the PI3K/AKT/mTOR signaling pathway. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-05-11 Xiaoyan Chu,Jun Lou,Yun Yi,Linlin Zhong,Ouping Huang
The mortality and morbidity rates of ovarian cancer (OC) are high, but the underlying mechanisms of OC have not been characterized. In this study, we determined the role of Rho GTPase Activating Protein 30 (ARHGAP30) in OC progression. We measured ARHGAP30 abundance in OC tissue samples and cells using immunohistochemistry (IHC) and RT-qPCR. EdU, transwell, and annexin V/PI apoptosis assays were used
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The role of miR-143-3p/FNDC1 axis on the progression of non-small cell lung cancer. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-05-03 Zhanshu Ma,Qi Gao,Wenjing Xin,Lei Wang,Yan Chen,Chang Su,Songyan Gao,Ruiling Sun
The study aimed to explore the functional role of fibronectin type III domain containing 1 (FNDC1) in nonsmall cell lung cancer (NSCLC), as well as the mechanism governing its expression. The expression levels of FNDC1 and related genes in tissue and cell samples were detected by qRT-PCR. Kaplan-Meier analysis was employed to analyze the association between FNDC1 level and the overall survival of NSCLC
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Clinicopathological assessment of PD-1/PD-L1 immune checkpoint expression in desmoid tumors. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-04-26 Kazuhiko Hashimoto,Shunji Nishimura,Yu Shinyashiki,Tomohiko Ito,Ryosuke Kakinoki,Masao Akagi
The details of immune molecules' expression in desmoid tumors (DTs) remain unclear. This study aimed to determine the expression status of the programmed death-1/programmed death ligand 1 (PD1/PD-L1) immune checkpoint mechanism in DTs. The study included patients with DTs (n=9) treated at our institution between April 2006 and December 2012. Immunostaining for CD4, CD8, PD-1, PD-L1, interleukin-2 (IL-2)
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Molecules involved in the sperm interaction in the human uterine tube: a histochemical and immunohistochemical approach. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-04-13 David Cajas,Emanuel Guajardo,Sergio Jara-Rosales,Claudio Nuñez,Renato Vargas,Victor Carriel,Antonio Campos,Luis Milla,Pedro Orihuela,Carlos Godoy-Guzman
In humans, even where millions of spermatozoa are deposited upon ejaculation in the vagina, only a few thousand enter the uterine tube (UT). Sperm transiently adhere to the epithelial cells lining the isthmus reservoir, and this interaction is essential in coordinating the availability of functional spermatozoa for fertilization. The binding of spermatozoa to the UT epithelium (mucosa) occurs due to
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Effects of piperlonguminine on lung injury in severe acute pancreatitis via the TLR4/NF-κB pathway. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-03-20 Qian Hu,Ran Tao,Xiaoyun Hu,Haibo Wu,Jianjun Xu
Acute pancreatitis is an inflammatory response in the pancreas, involving activation of pancreatic enzymes. Severe acute pancreatitis (SAP) often causes systemic complications that affect distant organs, including the lungs. The aim of this study was to explore the therapeutic potential of piperlonguminine on SAP-induced lung injury in rat models. Acute pancreatitis was induced in rats by repetitive
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Nrf2 as a novel diagnostic biomarker for papillary thyroid carcinoma. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-03-20 Zhiyang Wang,Jing Li,Ziwei Liu,Ling Yue
Papillary thyroid carcinoma (PTC) is the most common thyroid malignancy. However, it is very difficult to distinguish PTC from benign carcinoma. Thus, specific diagnostic biomarkers are actively pursued. Previous studies observed that Nrf2 was highly expressed in PTC. Based on this research, we hypothesized that Nrf2 may serve as a novel specific diagnostic biomarker. A single-center retrospective
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The Qi-Bang-Yi-Shen formula ameliorates renal dysfunction and fibrosis in rats with diabetic kidney disease via regulating PI3K/AKT, ERK and PPARγ signaling pathways. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-03-01 Zhi Wang,Guihua Jian,Teng Chen,Yiping Chen,Junhui Li,Niansong Wang
Diabetic kidney disease (DKD) is the leading cause of chronic kidney disease (CKD) and a growing public health problem worldwide. Losartan potassium (Los), an angiotensin II receptor blocker, has been used to treat DKD clinically. Recently, multi-herbal formula has been shown to exhibit therapeutic activities in DKD in China. Thus, we aimed to explore the protective effects of combination of Los and
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The contribution of immunohistochemistry to the development of hydrogels for skin repair and regeneration. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-02-23 Flavia Carton
Hydrogels based on various polymeric materials have been successfully developed in recent years for a variety of skin applications. Several studies have shown that hydrogels with regenerative, antibacterial, and antiinflammatory properties can provide faster and better healing outcomes, particularly in chronic diseases where the normal physiological healing process is significantly hampered. Various
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In memoriam of Prof. Stan Fakan. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-02-06 Marco Biggiogera,Manuela Malatesta
In memoriam of Prof. Stan Fakan.
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Effects of artificial light with different spectral composition on eye axial growth in juvenile guinea pigs. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-02-06 Xinyu Xu,Jiayu Shi,Chuanwei Zhang,Lixin Shi,Yujie Bai,Wei Shi,Yuliang Wang
The purpose of the study was to investigate the effect of artificial light with different spectral composition and distribution on axial growth in guinea pigs. Three-week-old guinea pigs were randomly assigned to groups exposed to natural light, low color temperature light-emitting diode (LED) light, two full spectrum artificial lights (E light and Julia light) and blue light filtered light with the
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LINC00926 is involved in hypoxia-induced vascular endothelial cell dysfunction via miR-3194-5p regulating JAK1/STAT3 signaling pathway. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-01-16 Yong Jiang,Chun-Hui Xu,Ying Zhao,Yun-Han Ji,Xin-Tao Wang,Ying Liu
Vascular endothelial cell (VEC) dysfunction is associated with the development of coronary heart disease (CHD). Long intergenic non-protein coding RNA 926 (LINC00926), a kind of long noncoding RNA (lncRNA), has been found to be abnormally expressed in CHD patients. However, the biological role of LINC00926 has not been reported. In our research, we intended to explore the regulatory mechanism of LINC00926
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Phosphorylation mutation impairs the promoting effect of spastin on neurite outgrowth without affecting its microtubule severing ability. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-01-12 Yunlong Zhang,Xin He,Jianyu Zou,Jie Yang,Ao Ma,Minghui Tan
Spastin, a microtubule-severing enzyme, is known to be important for neurite outgrowth. However, the role of spastin post-translational modification, particularly its phosphorylation regulation in neuronal outgrowth, remains unclear. This study aimed to investigate the effects of eliminating spastin phosphorylation on the neurite outgrowth of rat hippocampal neurons. To accomplish this, we constructed
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Neurokinin-1 receptor is highly expressed in cervical cancer and its antagonist induces cervical cancer cell apoptosis. Eur. J. Histochem. (IF 2.0) Pub Date : 2023-01-11 Liyun Guan,Shifa Yuan,Jing Ma,Hong Liu,Lizhen Huang,Fengzhen Zhang
Neurokinin-1 receptor (NK1R) belongs to tachykinin receptor family. Recent studies have suggested that NK1R was upregulated in cancer tissues including breast cancer, glioma and melanoma. Furthermore, NK1R antagonists have been employed to exert anti-tumor effect and promote cancer cell apoptosis. However, the role of NK1R in cervical cancer remains largely unknown. In this study, we aimed to detect
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Decreased VEGFA alleviates the symptoms of LPS-induced sepsis in a mouse model by inhibiting glycolysis and thereby regulating the polarization of macrophages. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-12-22 Jun Lin,Liping Jiang,Kun Guo,Ning Feng
The immune imbalance caused by excessive inflammatory reactions is the primary cause of sepsis. Macrophages with M1 and M2 polarization states are important immune cells that regulate the balance of the inflammatory response in sepsis. Encouraging the conversion of macrophages from the M1 to the M2 type is an important strategy for relieving sepsis. Here, we demonstrated the upregulation of vascular
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DPY30 promotes the growth and survival of osteosarcoma cell by regulating the PI3K/AKT signal pathway. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-12-22 Gong Cheng,Fengmin An,Zhilin Cao,Mingdi Zheng,Zhongyuan Zhao,Hao Wu
Osteosarcoma (OS) is characterized by aggressive features including invasiveness and high incidence of metastasis. OS patients with metastases are difficult to treat and suffer from a poor prognosis. DPY30 (protein dpy-30 homolog) is a key component of SET1/MLL family of H3K4 methyltransferases, which is implicated in the progression of multiple cancers. However, the potential functional engagement
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Growth hormone inhibits the JAK/STAT3 pathway by regulating SOCS1 in endometrial cells in vitro: a clue to enhance endometrial receptivity in recurrent implantation failure. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-12-22 Haixia Li,Ning Sun,Yaqiao Zhu,Wei Wang,Meihong Cai,Xiaohuan Luo,Wei Xia,Song Quan
Recurrent implantation failure (RIF) is defined as failure to achieve clinical pregnancy after at least 3 transfers of good-quality embryos by natural or artificial means. RIF is often a complex problem with a wide variety of etiologies and mechanisms as well as treatment options. In this study, using immunohistochemistry and Western blot, we demonstrated that the expression of leukemia inhibitory
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Differential antigen expression between human apocrine sweat glands and eccrine sweat glands. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-12-22 Manxiu Cao,Lei Zhang,Jiaqi Chen,Cangyu Wang,Junhong Zhao,Xiang Liu,Yongjing Yan,Yue Tang,Zixiu Chen,Haihong Li
Bromhidrosis has a great negative impact on personal occupation and social psychology. It is not yet clear whether bromhidrosis is caused by apocrine sweat glands or the co-action of apocrine sweat glands and eccrine sweat glands. To distinguish between apocrine sweat glands and eccrine sweat glands, specific antigen markers for apocrine sweat glands and eccrine sweat glands must be found first. In
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An ex vivo experimental system to track fluorescent nanoparticles inside skeletal muscle. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-12-22 Laura Calderan,Flavia Carton,Ilaria Andreana,Valeria Bincoletto,Silvia Arpicco,Barbara Stella,Manuela Malatesta
The development of novel nanoconstructs for biomedical applications requires the assessment of their biodistribution, metabolism and clearance in single cells, organs and entire organisms in a living environment. To reduce the number of in vivo experiments performed and to refine the methods used, in accordance with the 3Rs principle, this work proposes an ex vivo experimental system to monitor, using
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Is mastocytic colitis a specific clinical-pathological entity? Eur. J. Histochem. (IF 2.0) Pub Date : 2022-11-28 Filippo Vernia,Tiziana Tatti,Stefano Necozione,Annalisa Capannolo,Nicola Cesaro,Marco Magistroni,Marco Valvano,Simona Pompili,Roberta Sferra,Antonella Vetuschi,Giovanni Latella
The number of intestinal mast cells (MC) is increased in several types of colitis, but the mucosa of patients with chronic non-bloody diarrhea has not been studied. The current study sought to determine the relationship between MC counts and degranulation and the severity of symptoms in patients with chronic loose stools. Following a negative laboratory workup for the most common causes of chronic
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Assessing the interactions between nanoparticles and biological barriers in vitro: a new challenge for microscopy techniques in nanomedicine. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-11-24 Flavia Carton,Manuela Malatesta
Nanoconstructs intended to be used as biomedical tool must be assessed for their capability to cross biological barriers. However, studying in vivo the permeability of biological barriers to nanoparticles is quite difficult due to the many structural and functional factors involved. Therefore, the in vitro modeling of biological barriers -2D cell monocultures, 2D/3D cell co-cultures, microfluidic devices-
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Combination of tolvaptan and valsartan improves cardiac and renal functions in doxorubicin-induced heart failure in mice. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-11-11 Fengqin Yan,Hong Zhu,Yingxia He,Qinqin Wu,Xiaoyu Duan
Heart failure (HF) is often complicated by renal dysfunction. Tolvaptan and valsartan are two well-known agents for the treatment of HF. However, the role of tolvaptan/valsartan combination on HF with renal dysfunction remains unclear. To establish a mice model with HF with renal dysfunction, mice were intraperitoneally injected with doxorubicin (Dox). Echocardiogram was applied to assess the left
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Expression profile of the zinc transporter ZnT3 in taste cells of rat circumvallate papillae and its role in zinc release, a potential mechanism for taste stimulation. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-11-10 Kentaro Nishida,Saho Bansho,Akiko Ikukawa,Teruyo Kubota,Akihiro Ohishi,Kazuki Nagasawa
Zinc is an essential trace element, and its deficiency causes taste dysfunction. Zinc accumulates in zinc transporter (ZnT)3-expressing presynaptic vesicles in hippocampal neurons and acts as a neurotransmitter in the central nervous system. However, the distribution of zinc and its role as a signal transmitter in taste buds remain unknown. Therefore, we examined the distribution of zinc and expression
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SerpinB3 administration protects liver against ischemia-reperfusion injury. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-10-28 Cristian Turato,Mariapia Vairetti,Marta Cagna,Alessandra Biasiolo,Andrea Ferrigno,Santina Quarta,Mariagrazia Ruvoletto,Silvia De Siervi,Patrizia Pontisso,Laura Giuseppina Di Pasqua
We have investigated the change in SerpinB3 during hepatic ischemia and the potential role of its antiprotease activity in cell protection by the administration of wild-type SerpinB3 (SerpinB3-WT) or active loop-deleted recombinant SerpinB3 protein (SerpinB3-D) in a rat model of ischemia (60 min)/reperfusion (60 min) (I/R). A time-dependent increase of SerpinB3, both at transcription and protein level
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VEGF promotes diabetic retinopathy by upregulating the PKC/ET/NF-κB/ICAM-1 signaling pathway. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-10-27 Meiying Zhang,Min Zhou,Xia Cai,Yan Zhou,Xueling Jiang,Yan Luo,Yue Hu,Rong Qiu,Yanrong Wu,Yuejin Zhang,Yan Xiong
Diabetic retinopathy (DR) is a common microvascular complication in patients with diabetes mellitus. DR is caused by chronic hyperglycemia and is characterized by progressive loss of vision because of damage to the retinal microvasculature. In this study, we investigated the regulatory role and clinical significance of the vascular endothelial growth factor (VEGF)/protein kinase C (PKC)/endothelin
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Docetaxel-resistant triple-negative breast cancer cell-derived exosomal lncRNA LINC00667 reduces the chemosensitivity of breast cancer cells to docetaxel via targeting miR-200b-3p/Bcl-2 axis. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-10-25 Jindong Li,Jie Kang,Weiyan Liu,Jiazhe Liu,Gaofeng Pan,Anwei Mao,Qing Zhang,Jingfeng Lu,Junbin Ding,Hongchang Li
Development of docetaxel (TXT) resistance is a major obstacle for triple-negative breast cancer (TNBC) treatment. Additionally, chemoresistant cell-derived exosomes were able to change the chemo-response of chemosensitive recipient cells via transportation of lncRNAs. It has been shown that lncRNA LINC00667 level was significantly elevated in breast cancer tissues. Therefore, we explored whether LINC00667
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Tumor cell-derived exosomal lncRNA LOC441178 inhibits the tumorigenesis of esophageal carcinoma through suppressing macrophage M2 polarization. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-10-14 Jiangmu Chen,Zongchi Chen,Weitao Hu,Daxing Cai
Esophageal carcinoma (EC) is a highly malignant type of tumor. In a previous study, the authors found that long non-coding RNA (lncRNA) LOC441178 inhibited the tumorigenesis of EC. Moreover, exosomes derived from tumor cells containing lncRNAs were found to play a key role in the tumor environment; however, whether exosomes can affect the tumor microenvironment by carrying LOC441178 remains unclear
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Glyphosate, AMPA and glyphosate-based herbicide exposure leads to GFAP, PCNA and caspase-3 increased immunoreactive area on male offspring rat hypothalamus. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-10-13 Ewing Duque-Díaz,Hernán Hurtado Giraldo,Linda P Rocha-Muñoz,Rafael Coveñas
Glyphosate, aminomethylphosphonic acid (AMPA), and glyphosate-based herbicides altered the neuroendocrine axis, the content of brain neurotransmitters, and behavior in experimental animal models. Glyphosate alone, AMPA or Roundup® Active were administered to postpartum female rats, from P0 to P10, and their water consumption was measured daily. The immunoreactivity for glial fibrillary acidic protein
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Chemotherapy-induced nephrotoxicity was improved by crocin in mouse model. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-10-03 Qichao Yin,Hua Xiong
Cisplatin (CDDP) has been widely used in cancer therapy, but it has been linked to side effects such as nephrotoxicity. Crocin is a carotenoid found in crocus and gardenia flowers that has been shown to have anti-oxidant properties, inhibit tumor growth, and provide neuroprotection. The purpose of this study was to investigate the protective effect of crocin against CDDP-induced nephrotoxicity in a
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Overexpression of hsa_circ_0006470 inhibits the malignant behavior of gastric cancer cells via regulation of miR-1234/TP53I11 axis. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-10-03 Jinbi Xie,Yong Ning,Lihang Zhang,Yuan Lin,Runsheng Guo,Shanjuan Wang
Gastric cancer (GC) is a subtype of a common malignant tumor found in the digestive system. Hsa_circ_0006470 is known to be closely associated with the development of GC. Nevertheless, the mechanism by which hsa_circ_0006470 regulates the tumorigenesis of GC has not been fully elucidated. To investigate the role of hsa_circ_0006470 in GC, its expression levels were assessed in GES-1, AGS, MKN45, and
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alpha2,3 sialic acid processing enzymes expression in gastric cancer tissues reveals that ST3Gal3 but not Neu3 are associated with Lauren's classification, angiolymphatic invasion and histological grade. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-09-29 Michael W L Quirino,Amanda P B Albuquerque,Maria F D De Souza,Antônio F Da Silva Filho,Mário R Martins,Maira G Da Rocha Pitta,Michelly C Pereira,Moacyr J B De Melo Rêgo
Gastric cancer (GC) is one of the leading causes of cancer-related deaths worldwide. Despite progress in the last decades, there are still no reliable biomarkers for the diagnosis of and prognosis for GC. Aberrant sialylation is a widespread critical event in the development of GC. Neuraminidases (Neu) and sialyltransferases (STs) regulate the ablation and addition of sialic acid during glycoconjugates
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Polydatin reverses oxidation low lipoprotein (oxLDL)-induced apoptosis of human umbilical vein endothelial cells via regulating the miR-26a-5p/BID axis. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-09-20 Dajie Wang,Zhaofeng Zhou,Liang Yuan
Atherosclerosis is a disease in which lipids and inflammatory factors accumulate on the walls of arteries, forming plaques that eventually block the flow of blood. Polydatin was derived from plant knotweed, which could play an important role in inhibiting the progression of atherosclerosis. However, the mechanism by which polydatin regulates the genesis and development of atherosclerosis remains unclear
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Imaging and spectral analysis of autofluorescence patterns in larval head structures of mosquito vectors. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-09-20 Francesca Scolari,Alessandro Girella,Anna Cleta Croce
Autofluorescence (AF) in mosquitoes is currently poorly explored, despite its great potential as a marker of body structures and biological functions. Here, for the first time AF in larval heads of two mosquitoes of key public health importance, Aedes albopictus and Culex pipiens, is studied using fluorescence imaging and spectrofluorometry, similarly to a label-free histochemical approach. In generally
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In memoriam of Prof. GianCarlo Panzica. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-09-02 Editor In Chief
In memoriam of Prof. GianCarlo Panzica.
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Long non-coding RNA WAC antisense RNA 1 mediates hepatitis B virus replication in vitro by reinforcing miR-192-5p/ATG7-induced autophagy. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-09-02 Minkai Cao,Deping Yuan,Hongxiu Jiang,Guanlun Zhou,Chao Chen,Guorong Han
Long non-coding RNA WAC antisense RNA 1 (lncRNA WAC-AS1) is involved in the replication of the hepatitis B virus (HBV). The purpose of this study was to determine its functions and specific mechanism. The levels of lncRNA WAC-AS1, RNA (miR)-192-5p and were examined in serum of HBV-infected patients and in HepG2.2.15 cells using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR)
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Immunofluorescence signal intensity measurements as a semi-quantitative tool to assess sarcoglycan complex expression in muscle biopsy. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-09-01 Simona Zanotti,Francesca Magri,Francesca Poggetti,Michela Ripolone,Daniele Velardo,Francesco Fortunato,Patrizia Ciscato,Maurizio Moggio,Stefania Corti,Giacomo Pietro Comi,Monica Sciacco
Sarcoglycanopathies are highly heterogeneous in terms of disease progression, muscular weakness, loss of ambulation and cardiac/respiratory involvement. Their clinical severity usually correlates with the residual protein amount, which makes protein quantification extremely relevant. Sarcoglycanopathy diagnosis is genetic, but skeletal muscle analysis - by both immunohistochemistry and Western blot
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Inhibition of APLN suppresses cell proliferation and migration and promotes cell apoptosis in esophageal cancer cells in vitro, through activating PI3K/mTOR signaling pathway. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-08-03 Yuhan Wang,Gang Wang,Xiaojun Liu,Dong Yun,Qing Cui,Xiaoting Wu,Wenfeng Lu,Xiwen Yang,Ming Zhang
Esophageal cancer is the sixth leading cause of cancer mortalities globally with a high incidence rate. Apelin (APLN) plays regulatory roles in different organs. However, its role in esophageal cancer remains unknown. Therefore, our study aims to explore the effect of APLN on esophageal cancer. One hundred and eighty-four (184) esophageal tumor tissues samples from patients with esophageal cancer,
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MiR-550a-3p restores damaged vascular smooth muscle cells by inhibiting thrombomodulin in an in vitro atherosclerosis model. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-07-19 Shiyuan Chen,Longfei Zhang,Benchi Feng,Wei Wang,Delang Liu,Xinyu Zhao,Chaowen Yu,Xiaogao Wang,Yong Gao
Thrombomodulin (TM) is involved in the pathological process of atherosclerosis; however, the underlying mechanism remains unclear. Oxidised low-density lipoprotein (Ox-LDL; 100 μg/mL) was used to induce human vascular smooth muscle cells (HVSMCs) into a stable atherosclerotic cell model. The expression levels of miR-550a-3p and TM were detected by real-time reverse transcription-polymerase chain reaction
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Lobetyolin suppressed lung cancer in a mouse model by inhibiting epithelial-mesenchymal transition. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-07-04 Lu Liu,Zhankui Liu,Liu Yang,Xue Wu,Jiaying Zhu,Lili Liu,Yang Liu
Traditional Chinese medicines are gaining more attention as promising adjuvant agents for conventional chemotherapy. Recent studies have shown that lobetyolin (LBT) is one of the main bioactive compounds of traditional Chinese medicines and it exhibits anticancer activity in several types of cancer. Therefore, this study aimed to investigate the mechanism by which LBT inhibits lung cancer. A549 human
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Clinicopathological assessment of cancer/testis antigens NY‑ESO‑1 and MAGE‑A4 in osteosarcoma. Eur. J. Histochem. (IF 2.0) Pub Date : 2022-06-23 Kazuhiko Hashimoto,Shunji Nishimura,Tomohiko Ito,Naohiro Oka,Ryosuke Kakinoki,Masao Akagi
The cancer/testis antigens (CTAs), New York esophageal squamous cell carcinoma-1 (NY-ESO-1) and melanoma antigen gene (MAGE)-A4 are normally restricted to male germ cells but are aberrantly expressed in several cancers. Considering the limited information regarding their significance in osteosarcoma (OS), the purpose of this study was to determine the clinical significance of NY-ESO-1 and MAGE-A4 expression