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  • LSP1‐myosin1e bimolecular complex regulates focal adhesion dynamics and cell migration
    FASEB J. (IF 4.966) Pub Date : 2021-01-20
    Katja Schäringer; Sebastian Maxeiner; Carmen Schalla; Stephan Rütten; Martin Zenke; Antonio Sechi

    Several cytoskeleton‐associated proteins and signaling pathways work in concert to regulate actin cytoskeleton remodeling, cell adhesion, and migration. Although the leukocyte‐specific protein 1 (LSP1) has been shown to interact with the actin cytoskeleton, its function in the regulation of actin cytoskeleton dynamics is, as yet, not fully understood. We have recently demonstrated that the bimolecular

    更新日期:2021-01-20
  • Conserved C‐terminal motifs in odorant receptors instruct their cell surface expression and cAMP signaling
    FASEB J. (IF 4.966) Pub Date : 2021-01-19
    Matthias Kotthoff; Julia Bauer; Franziska Haag; Dietmar Krautwurst

    The highly individual plasma membrane expression and cAMP signaling of odorant receptors have hampered their ligand assignment and functional characterization in test cell systems. Chaperones have been identified to support the cell surface expression of only a portion of odorant receptors, with mechanisms remaining unclear. The presence of amino acid motifs that might be responsible for odorant receptors'

    更新日期:2021-01-19
  • NPM1 mutant maintains ULK1 protein stability via TRAF6‐dependent ubiquitination to promote autophagic cell survival in leukemia
    FASEB J. (IF 4.966) Pub Date : 2020-11-17
    Yuting Tang; Yao Tao; Lu Wang; Liyuan Yang; Yipei Jing; Xueke Jiang; Li Lei; Zailin Yang; Xin Wang; Meixi Peng; Qiaoling Xiao; Jun Ren; Ling Zhang

    Nucleophosmin (NPM1) mutations are the most frequent genetic alteration in acute myeloid leukemia (AML) and aberrant cytoplasm‐dislocated NPM1 mutant is a distinct biological characterization of this disease. Our group previously reported that NPM1 mutant elevated autophagy activity and autophagy activation contributed to leukemic cell survival. However, the molecular mechanisms by which cytoplasmic

    更新日期:2021-01-18
  • Modulation of microRNA processing by 5‐lipoxygenase
    FASEB J. (IF 4.966) Pub Date : 2020-11-17
    Stella Uebbing; Marius Kreiß; Friederike Scholl; Ann‐Kathrin Häfner; Duran Sürün; Ulrike Garscha; Oliver Werz; Devaraj Basavarajappa; Bengt Samuelsson; Olof Rådmark; Beatrix Suess; Dieter Steinhilber

    The miRNA biogenesis is tightly regulated to avoid dysfunction and consequent disease development. Here, we describe modulation of miRNA processing as a novel noncanonical function of the 5‐lipoxygenase (5‐LO) enzyme in monocytic cells. In differentiated Mono Mac 6 (MM6) cells, we found an in situ interaction of 5‐LO with Dicer, a key enzyme in miRNA biogenesis. RNA sequencing of small noncoding RNAs

    更新日期:2021-01-18
  • Microglial ablation in rats disrupts the circadian system
    FASEB J. (IF 4.966) Pub Date : 2020-11-16
    Luba Sominsky; Tamara Dangel; Sajida Malik; Simone N. De Luca; Nicolas Singewald; Sarah J. Spencer

    Microglia, the key neuroimmune cells of the central nervous system, are best known for their function in defending an individual from pathogens and injury. Recent findings, including our own, suggest microglia also have several immune‐independent roles, including in regulating satiety, promoting memory, and modifying pain responses. Many of these microglia‐associated functions are affected by circadian

    更新日期:2021-01-18
  • Defining the regenerative effects of native spider silk fibers on primary Schwann cells, sensory neurons, and nerve‐associated fibroblasts
    FASEB J. (IF 4.966) Pub Date : 2020-11-19
    Flavia Millesi; Tamara Weiss; Anda Mann; Maximilian Haertinger; Lorenz Semmler; Paul Supper; Dietmar Pils; Aida Naghilou; Christine Radtke

    The search for a suitable material to promote regeneration after long‐distance peripheral nerve defects turned the spotlight on spider silk. Nerve conduits enriched with native spider silk fibers as internal guiding structures previously demonstrated a regenerative outcome similar to autologous nerve grafts in animal studies. Nevertheless, spider silk is a natural material with associated limitations

    更新日期:2021-01-18
  • Long‐term consumption of recycled cooking oil induces cell death and tissue damage
    FASEB J. (IF 4.966) Pub Date : 2020-11-19
    Shudong Zhu; Yan Zhu; Hao Li; Qiuwen Wang; Kuansong Wang; Katelynn Baska; Dianzheng Zhang

    Recycled cooking oil (RCO) is widely used in many small restaurants. However, the health risk posed by long‐term consumption of RCO is unclear. In this study, C57 mice were treated with RCO for 34 weeks. Organ coefficients of the liver, stomach, and kidney were found to be decreased. H&E staining revealed overt lesions in the pancreas, liver, kidney, esophagus, duodenum, and ileum of RCO‐treated mice

    更新日期:2021-01-18
  • Endometrial epithelial ARID1A is critical for uterine gland function in early pregnancy establishment
    FASEB J. (IF 4.966) Pub Date : 2020-11-22
    Ryan M. Marquardt; Tae Hoon Kim; Jung‐Yoon Yoo; Hanna E. Teasley; Asgerally T. Fazleabas; Steven L. Young; Bruce A. Lessey; Ripla Arora; Jae‐Wook Jeong

    Though endometriosis and infertility are clearly associated, the pathophysiological mechanism remains unclear. Previous work has linked endometrial ARID1A loss to endometriosis‐related endometrial non‐receptivity. Here, we show in mice that ARID1A binds and regulates transcription of the Foxa2 gene required for endometrial gland function. Uterine‐specific deletion of Arid1a compromises gland development

    更新日期:2021-01-18
  • Tiam1 mediates Rac1 activation and contraction‐induced glucose uptake in skeletal muscle cells
    FASEB J. (IF 4.966) Pub Date : 2020-11-22
    Yingying Yue; Chang Zhang; Xiaoyun Zhao; Sasa Liu; Xiaoting Lv; Shitian Zhang; Jianming Yang; Liming Chen; Hongquan Duan; Youyi Zhang; Zhi Yao; Wenyan Niu

    Contraction‐stimulated glucose uptake in skeletal muscle requires Rac1, but the molecular mechanism of its activation is not fully understood. Treadmill running was applied to induce C57BL/6 mouse hind limb skeletal muscle contraction in vivo and electrical pulse stimulation contracted C2C12 myotube cultures in vitro. The protein levels or activities of AMPK or the Rac1‐specific GEF, Tiam1, were manipulated

    更新日期:2021-01-18
  • Timing of proteasome inhibition as a pharmacologic strategy for prevention of muscle contractures in neonatal brachial plexus injury
    FASEB J. (IF 4.966) Pub Date : 2020-11-25
    Qingnian Goh; Sia Nikolaou; Kritton Shay‐Winkler; Marianne E. Emmert; Roger Cornwall

    Neonatal brachial plexus injury (NBPI) causes disabling and incurable contractures, or limb stiffness, which result from proteasome‐mediated protein degradation impairing the longitudinal growth of neonatally denervated muscles. We recently showed in a mouse model that the 20S proteasome inhibitor, bortezomib, prevents contractures after NBPI. Given that contractures uniquely follow neonatal denervation

    更新日期:2021-01-18
  • Functional advantages of triplication of the 3B coding region of the FMDV genome
    FASEB J. (IF 4.966) Pub Date : 2020-11-23
    Oluwapelumi O. Adeyemi; Joseph C. Ward; Joseph S. Snowden; Morgan R. Herod; David J. Rowlands; Nicola J. Stonehouse

    For gene duplication to be maintained, particularly in the small genomes of RNA viruses, this should offer some advantages. We have investigated the functions of a small protein termed VPg or 3B, which acts as a primer in the replication of foot‐and‐mouth disease virus (FMDV). Many related picornaviruses encode a single copy but uniquely the FMDV genome includes three (nonidentical) copies of the 3B

    更新日期:2021-01-18
  • Hyperleptinemia as a contributing factor for the impairment of glucose intolerance in obesity
    FASEB J. (IF 4.966) Pub Date : 2020-11-23
    Dominik Pretz; Christelle Le Foll; Mohammed Z. Rizwan; Thomas A. Lutz; Alexander Tups

    Obesity has emerged as a major risk factor for insulin resistance leading to the development of type 2 diabetes (T2D). The condition is characterized by high circulating levels of the adipose‐derived hormone leptin and a state of chronic low‐grade inflammation. Pro‐inflammatory signaling in the hypothalamus is associated with a decrease of central leptin‐ and insulin action leading to impaired systemic

    更新日期:2021-01-18
  • Collagen release by human hepatic stellate cells requires vitamin C and is efficiently blocked by hydroxylase inhibition
    FASEB J. (IF 4.966) Pub Date : 2020-11-24
    Natalia Smith‐Cortinez; Raphael R. Fagundes; Valentina Gomez; Defu Kong; Dirk R. de Waart; Janette Heegsma; Svenja Sydor; Peter Olinga; Vincent E. de Meijer; Cormac T. Taylor; Ruud Bank; Coen C. Paulusma; Klaas Nico Faber

    Liver fibrosis is characterized by the accumulation of extracellular matrix proteins, mainly composed of collagen. Hepatic stellate cells (HSCs) mediate liver fibrosis by secreting collagen. Vitamin C (ascorbic acid) is a cofactor of prolyl‐hydroxylases that modify newly synthesized collagen on the route for secretion. Unlike most animals, humans cannot synthesize ascorbic acid and its role in liver

    更新日期:2021-01-18
  • Rats deficient in the GAD65 isoform exhibit epilepsy and premature lethality
    FASEB J. (IF 4.966) Pub Date : 2020-11-25
    Toshikazu Kakizaki; Tomokazu Ohshiro; Makoto Itakura; Kohtarou Konno; Masahiko Watanabe; Hajime Mushiake; Yuchio Yanagawa

    GABA is synthesized by glutamate decarboxylase (GAD), which has two isoforms, namely, GAD65 and GAD67, encoded by the Gad2 and Gad1 genes, respectively. GAD65‐deficient (Gad2−/−) mice exhibit a reduction in brain GABA content after 1 month of age and show spontaneous seizures in adulthood. Approximately 25% of Gad2−/− mice died by 6 months of age. Our Western blot analysis demonstrated that the protein

    更新日期:2021-01-18
  • Development of a rabbit model of Wiskott‐Aldrich syndrome
    FASEB J. (IF 4.966) Pub Date : 2020-11-25
    Juanjuan Zhou; Quanmei Yan; Chengcheng Tang; Yuan Liao; Quanjun Zhang; Xiaomin Wang; Xiaoqing Zhou; Liangxue Lai; Qingjian Zou

    The Wiskott‐Aldrich syndrome (WAS) is a severe recessive X‐linked immunodeficiency resulting from loss‐of‐function mutations in the WAS gene. Mouse is the only mammalian model used for investigation of WAS pathogenesis. However, the mouse model does not accurately recapitulate WAS clinical phenotypes, thus, limiting its application in WAS clinical research. Herein, we report the generation of WAS knockout

    更新日期:2021-01-18
  • Unexpected obesity, rather than tumorigenesis, in a conditional mouse model of mitochondrial complex II deficiency
    FASEB J. (IF 4.966) Pub Date : 2020-11-27
    Fatimah Al Khazal; Seungwoo Kang; Molly Nelson Holte; Doo‐Sup Choi; Ravinder Singh; Patricia Ortega‐Sáenz; José López‐Barneo; L. James Maher

    Mutations in any of the genes encoding the four subunits of succinate dehydrogenase (SDH), a mitochondrial membrane‐bound enzyme complex that is involved in both the tricarboxylic acid cycle and the electron transport chain, can lead to a variety of disorders. Recognized conditions with such mutations include Leigh syndrome and hereditary tumors such as pheochromocytoma and paraganglioma (PPGL), renal

    更新日期:2021-01-18
  • Neural cell‐derived plasma exosome protein abnormalities implicate mitochondrial impairment in first episodes of psychosis
    FASEB J. (IF 4.966) Pub Date : 2021-01-17
    Edward J. Goetzl; Vinod H. Srihari; Sinan Guloksuz; Maria Ferrara; Cenk Tek; George R. Heninger

    Neuroprotective and other functional proteins of mitochondria were quantified in extracts of plasma neural‐derived exosomes from ten first‐episode psychosis (FP) patients and ten matched psychiatrically normal controls (ctls). Astrocyte‐derived extracellular vesicles (ADEVs) and neuron‐derived extracellular vesicles (NDEVs) were immunoabsorbed separately from physically precipitated plasma total EVs

    更新日期:2021-01-18
  • Physiological effects of inactivation and the roles of Elovl3/ELOVL3 in maintaining ocular homeostasis
    FASEB J. (IF 4.966) Pub Date : 2021-01-17
    Amber Wilkerson; Nita Bhat; Hoang Quoc Hai Pham; Seher Yuksel; Igor Butovich

    Recently, elongase of very long chain fatty acids‐3 (ELOVL3) was demonstrated to play a pivotal role in physiology and biochemistry of the ocular surface by maintaining a proper balance in the lipid composition of meibum. The goal of this study was to further investigate the effects of ELOVL3 ablation in homozygous Elovl3‐knockout mice (E3hom) in comparison with age and sex matched wild‐type controls

    更新日期:2021-01-18
  • Moderate aerobic exercise training ameliorates impairment of mitochondrial function and dynamics in skeletal muscle of high‐fat diet‐induced obese mice
    FASEB J. (IF 4.966) Pub Date : 2021-01-17
    Jun‐Won Heo; Mi‐Hyun No; Jinkyung Cho; Youngju Choi; Eun‐Jeong Cho; Dong‐Ho Park; Tae‐Woon Kim; Chang‐Ju Kim; Dae Yun Seo; Jin Han; Young C. Jang; Su‐Jeen Jung; Ju‐Hee Kang; Hyo‐Bum Kwak

    The purpose of this study is to determine whether moderate aerobic exercise training improves high‐fat diet‐induced alterations in mitochondrial function and structure in the skeletal muscle. Male 4‐week‐old C57BL/6 mice were randomly divided into four groups: control (CON), control plus exercise (CON + EX), high‐fat diet (HFD), and high‐fat diet plus exercise (HFD + EX). After obesity was induced

    更新日期:2021-01-18
  • Integrative cardiovascular control in women: Regulation of blood pressure, body temperature, and cerebrovascular responsiveness
    FASEB J. (IF 4.966) Pub Date : 2020-11-05
    Jill N. Barnes; Nisha Charkoudian

    Over the past several decades, it has become increasingly clear that women have distinct cardiovascular profiles compared to men. In this review, our goal is to provide an overview of the literature regarding the influences of female sex and reproductive hormones (primarily estradiol) on mechanisms of cardiovascular control relevant to regulation of blood pressure, body temperature, and cerebral blood

    更新日期:2021-01-14
  • Transcription factor old astrocyte specifically induced substance is a novel regulator of kidney fibrosis
    FASEB J. (IF 4.966) Pub Date : 2020-11-05
    Ayaha Yamamoto; Hitomi Morioki; Takafumi Nakae; Yoshiaki Miyake; Takeo Harada; Shunsuke Noda; Sayuri Mitsuoka; Kotaro Matsumoto; Masashi Tomimatsu; Soshi Kanemoto; Shota Tanaka; Makiko Maeda; Simon J. Conway; Kazunori Imaizumi; Yasushi Fujio; Masanori Obana

    Prevention of kidney fibrosis is an essential requisite for effective therapy in preventing chronic kidney disease (CKD). Here, we identify Old astrocyte specifically induced substance (OASIS)/cAMP responsive element‐binding protein 3‐like 1 (CREB3l1), a CREB/ATF family transcription factor, as a candidate profibrotic gene that drives the final common pathological step along the fibrotic pathway in

    更新日期:2021-01-14
  • Erratum
    FASEB J. (IF 4.966) Pub Date : 2020-11-12

    Correction for “Hepatic signaling by the mechanistic target of rapamycin complex 2 (mTORC2),” by Dudley W. Lamming, Gokhan Demirkan, Joan M. Boylan, Maria M. Mihaylova, Tao Peng Jonathan Ferreira, Nicola Neretti, Arthur Salomon, David M. Sabatini, and Philip A. Gruppuso, which was first published September 26, 2013; 10.1096/fj.13‐237743 (FASEB J. 28, 300–315). The authors note that there is an error

    更新日期:2021-01-14
  • Erratum
    FASEB J. (IF 4.966) Pub Date : 2020-11-22

    Correction for “TLR4‐MAP4K4 signaling pathway in models of oxygen‐induced retinopathy,” by Wenwen Chen, Juan Zhang, Peijun Zhang, Fangyuan Hu, Tingting Jiang, Junxiang Gu, and Qing Chang, which was first published in November 26, 2018; 10.1096/fj.201801086R (FASEB J. 33, 3451–3464). The authors note that there is a composition error in Figure 7. In the color keys for the bar graphs, the identifying

    更新日期:2021-01-14
  • Cyclin G2 upregulation impairs migration, invasion, and network formation through RNF123/Dvl2/JNK signaling in the trophoblast cell line HTR8/SVneo, a possible role in preeclampsia
    FASEB J. (IF 4.966) Pub Date : 2020-11-17
    Manni Sun; Jinlan Gao; Tao Meng; Shenghuan Liu; Haiying Chen; Qi Liu; Xuesha Xing; Chenyang Zhao; Yang Luo

    Disruption of extravillous trophoblast (EVT) migration and invasion is considered to be responsible for pathological placentation in preeclampsia (PE). Cyclin G2 (CCNG2) is an atypical cyclin that inhibits cell cycle progression. However, its biological function and underlying molecular mechanism in PE are poorly understood. In this study, clinical data demonstrated that CCNG2 was significantly upregulated

    更新日期:2021-01-14
  • Porphyromonas gingivalis impairs glucose uptake in skeletal muscle associated with altering gut microbiota
    FASEB J. (IF 4.966) Pub Date : 2020-11-16
    Kazuki Watanabe; Sayaka Katagiri; Hirokazu Takahashi; Naoki Sasaki; Shogo Maekawa; Rina Komazaki; Masahiro Hatasa; Yoichiro Kitajima; Yusuke Maruyama; Takahiko Shiba; Keiji Komatsu; Yujin Ohsugi; Kenichi Tanaka; Ayumi Matsuzawa; Tomomitsu Hirota; Haruka Tohara; Yuichiro Eguchi; Keizo Anzai; Atsuhiko Hattori; Takanori Iwata

    Skeletal muscles have a high metabolic capacity, which play key roles in glucose metabolism. Although periodontal disease increases the risk of metabolic syndrome, the relationship between periodontal bacterial infection and skeletal muscle metabolic dysfunction is unclear. We found that anti‐Porphyromonas gingivalis (Pg) antibody titers positively correlated with intramuscular adipose tissue content

    更新日期:2021-01-14
  • Blood‐brain barrier transport using a high affinity, brain‐selective VNAR antibody targeting transferrin receptor 1
    FASEB J. (IF 4.966) Pub Date : 2020-11-25
    Pawel Stocki; Jaroslaw Szary; Charlotte L. M. Rasmussen; Mykhaylo Demydchuk; Leandra Northall; Diana Bahu Logan; Aziz Gauhar; Laura Thei; Torben Moos; Frank S. Walsh; J. Lynn Rutkowski

    Transfer across the blood‐brain barrier (BBB) remains a significant hurdle for the development of biopharmaceuticals with therapeutic effects within the central nervous system. We established a functional selection method to identify high affinity single domain antibodies to the transferrin receptor 1 (TfR1) with efficient biotherapeutic delivery across the BBB. A synthetic phage display library based

    更新日期:2021-01-14
  • Alternate thermal stimulation ameliorates thermal sensitivity and modulates calbindin‐D 28K expression in lamina I and II and dorsal root ganglia in a mouse spinal cord contusion injury model
    FASEB J. (IF 4.966) Pub Date : 2020-11-23
    Xing Cheng; Fan Xiao; Rong Xie; Haijun Hu; Yong Wan

    Neuropathic pain (NP) is a common complication that negatively affects the lives of patients with spinal cord injury (SCI). The disruption in the balance of excitatory and inhibitory neurons in the spinal cord dorsal horn contributes to the development of SCI and induces NP. The calcium‐binding protein (CaBP) calbindin‐D 28K (CaBP‐28K) is highly expressed in excitatory interneurons, and the CaBP parvalbumin

    更新日期:2021-01-14
  • Brain iron deficiency and affected contextual fear memory in mice with conditional Ferroportin1 ablation in the brain
    FASEB J. (IF 4.966) Pub Date : 2020-11-16
    Qiong Wu; Qian Hao; Haiyan Li; Bo Wang; Peina Wang; Xiaofang Jin; Peng Yu; Guofen Gao; Yan‐Zhong Chang

    Fear memory is a pivotal biological function by which organisms can predict possible danger to avoid or reduce harm. However, dysregulation of fear memory processing may lead to pathological fear or anxiety and produce serious clinical symptoms, such as post‐traumatic stress disorder (PTSD). Iron deficiency (ID) is reported to inhibit the initiation of fear memory. In our study, we found that ferroportin1

    更新日期:2021-01-14
  • Spatial organization and crosstalk of vimentin and actin stress fibers regulate the osteogenic differentiation of human adipose‐derived stem cells
    FASEB J. (IF 4.966) Pub Date : 2020-11-17
    Tingyu Fan; Rongmei Qu; Xin Jiang; Yuchao Yang; Bing Sun; Xiaolan Huang; Zhitao Zhou; Jun Ouyang; Shizhen Zhong; Jingxing Dai

    Human adipose‐derived stem cells (hASCs) are ideal seed cells for tissue engineering due to their multidirectional differentiation potential. Microfilaments, microtubules, and intermediate filaments are responsible for supporting the intracellular space. Vimentin, a type III intermediate filament protein that is specifically expressed in cells of mesenchymal origin, can function as a scaffold and endow

    更新日期:2021-01-14
  • A SNARE protein Syntaxin 17 captures CFTR to potentiate autophagosomal clearance under stress
    FASEB J. (IF 4.966) Pub Date : 2020-11-15
    Kavisha Arora; Pramodha Liyanage; Qing Zhong; Anjaparavanda P. Naren

    Autophagy, a cellular stress response to starvation and bacterial infection, is executed by double‐membrane‐bound organelles called autophagosomes. Autophagosomes transfer cytosolic material to acidified lysosomes for degradation following soluble N‐ethylmaleimide‐sensitive factor attachment receptor (SNARE)‐dependent fusion processes. Many of the autophagy‐related disorders stem from defective end‐step

    更新日期:2021-01-14
  • Non‐pigmented ciliary epithelium derived extracellular vesicles uptake mechanism by the trabecular meshwork
    FASEB J. (IF 4.966) Pub Date : 2020-11-16
    Saray Tabak; Uzi Hadad; Sofia Schreiber‐Avissar; Elie Beit‐Yannai

    Consistent with increasing findings, extracellular vesicles (EVs), consider as a major constituents of the aqueous humor, have a role as signaling mediators in glaucoma. Following secretion, EVs hold immense promise for utilization as bio‐therapeutics and drug delivery vehicles due to their nature as biological nanoparticles that facilitate intercellular molecular transport. Yet, the specific pathway

    更新日期:2021-01-14
  • Lin 28A/Occludin axis: An aberrantly activated pathway in intestinal epithelial cells leading to impaired barrier function under total parenteral nutrition
    FASEB J. (IF 4.966) Pub Date : 2020-11-16
    Yang Liu; Zizhen Gong; Jiefei Zhou; Junkai Yan; Wei Cai

    Disassembly of tight junctions is a major cause of intestinal barrier dysfunction under total parenteral nutrition (TPN), but the precise mechanisms have not been fully understood. Normally, RNA binding protein Lin 28A is highly restricted to embryonic stem cells and dramatically decreases as differentiation progresses; however, in our preliminary study it was found aberrantly increased in the intestinal

    更新日期:2021-01-14
  • Women, opioid use and addiction
    FASEB J. (IF 4.966) Pub Date : 2021-01-12
    Teddy G. Goetz; Jill B. Becker; Carolyn M. Mazure

    In the midst of the current coronavirus pandemic, the United States continues to struggle with an ongoing opioid epidemic, initially fueled by widespread prescribing of opioid medications during the 1990s. The primary reason for prescribing opioids is to treat pain. Women have more acute and chronic pain and have been prescribed these drugs in significantly greater numbers than men. Comparison of women

    更新日期:2021-01-12
  • Sex‐based differences after a single bout of exercise on PGC1α isoforms in skeletal muscle: A pilot study
    FASEB J. (IF 4.966) Pub Date : 2021-01-12
    Daniela D'Amico; Antonella Marino Gammazza; Filippo Macaluso; Letizia Paladino; Federica Scalia; Giulio Spinoso; Ivan Dimauro; Daniela Caporossi; Francesco Cappello; Valentina Di Felice; Rosario Barone

    To date, there are limited and incomplete data on possible sex‐based differences in fiber‐types of skeletal muscle and their response to physical exercise. Adult healthy male and female mice completed a single bout of endurance exercise to examine the sex‐based differences of the peroxisome proliferator‐activated receptor gamma coactivator 1 alpha (PGC1α), heat shock protein 60 (Hsp60), interleukin

    更新日期:2021-01-12
  • p53‐mediated regulation of mitochondrial dynamics plays a pivotal role in the senescence of various normal cells as well as cancer cells
    FASEB J. (IF 4.966) Pub Date : 2021-01-12
    Young Yeon Kim; Jee‐Hyun Um; Dong Jin Shin; Dae Jin Jeong; Young Bin Hong; Jeanho Yun

    The tumor suppressor p53 is known as a critical mediator of many cellular processes, including cellular senescence, but its role in mitochondrial dynamics is not fully understood. We have previously shown that p53 regulates mitochondrial dynamics via the PKA‐Drp1 pathway to induce cellular senescence. In this study, to further understand the role of p53‐dependent regulation of mitochondrial dynamics

    更新日期:2021-01-12
  • Butyrate drives the acetylation of histone H3K9 to activate steroidogenesis through PPARγ and PGC1α pathways in ovarian granulosa cells
    FASEB J. (IF 4.966) Pub Date : 2021-01-12
    Qianhong Ye; Xiangfang Zeng; Shuai Wang; Xiangzhou Zeng; Guangxin Yang; Changchuan Ye; Shuang Cai; Meixia Chen; Siyu Li; Shiyan Qiao

    Maintaining ovarian steroidogenesis is of critical importance, considering that steroid hormones are required for successful establishment and maintenance of pregnancy and proper development of embryos and fetuses. Investigating the mechanism that butyrate modulates the ovarian steroidogenesis is beneficial for understanding the impact of lipid nutrition on steroidogenesis. Herein, we identified that

    更新日期:2021-01-12
  • Nanoparticle‐encapsulated antioxidant improves placental mitochondrial function in a sexually dimorphic manner in a rat model of prenatal hypoxia
    FASEB J. (IF 4.966) Pub Date : 2021-01-11
    Esha Ganguly; Raven Kirschenman; Floor Spaans; Claudia D. Holody; Thomas E. J. Phillips; C. Patrick Case; Christy‐Lynn M. Cooke; Michael P. Murphy; Hélène Lemieux; Sandra T. Davidge

    Pregnancy complications associated with prenatal hypoxia lead to increased placental oxidative stress. Previous studies suggest that prenatal hypoxia can reduce mitochondrial respiratory capacity and mitochondrial fusion, which could lead to placental dysfunction and impaired fetal development. We developed a placenta‐targeted treatment strategy using a mitochondrial antioxidant, MitoQ, encapsulated

    更新日期:2021-01-11
  • Novel immortalized human vocal fold epithelial cell line: In vitro tool for mucosal biology
    FASEB J. (IF 4.966) Pub Date : 2021-01-11
    Xia Chen; Vlasta Lungova; Haiyan Zhang; Chitrasen Mohanty; Christina Kendziorski; Susan L. Thibeault

    Study of vocal fold (VF) mucosal biology requires essential human vocal fold epithelial cell (hVFE) lines for use in appropriate model systems. We steadily transfected a retroviral construct containing human telomerase reverse transcriptase (hTERT) into primary normal hVFE to establish a continuously replicating hVFE cell line. Immortalized hVFE across passages have cobblestone morphology, express

    更新日期:2021-01-11
  • Temporal regulation of tumor growth in nocturnal mammals: In vivo studies and chemotherapeutical potential
    FASEB J. (IF 4.966) Pub Date : 2021-01-11
    Paula M. Wagner; César G. Prucca; Fabiola N. Velazquez; Lucas G. Sosa Alderete; Beatriz L. Caputto; Mario E. Guido

    Tumors of the nervous system including glioblastoma multiforme (GBM) are the most frequent and aggressive form of brain tumors; however, little is known about the impact of the circadian timing system on the formation, growth, and treatment of these tumors. We investigated day/night differences in tumor growth after injection of A530 glioma cells isolated from malignant peripheral nerve sheath tumor

    更新日期:2021-01-11
  • Functional lipidomics of vascular endothelial cells in response to laminar shear stress
    FASEB J. (IF 4.966) Pub Date : 2021-01-09
    Tsuyoshi Hirata; Kimiko Yamamoto; Kazutaka Ikeda; Makoto Arita

    Laminar shear stress generated by blood flow stimulates endothelial cells and activates signal transduction, which plays an important role in vascular homeostasis. Several lines of evidence indicate that membrane and intracellular lipids are involved in the signal transduction of biomechanical stresses. In this study, we performed global profiling of cellular lipids from human pulmonary artery endothelial

    更新日期:2021-01-10
  • CircARID1A regulates mouse skeletal muscle regeneration by functioning as a sponge of miR‐6368
    FASEB J. (IF 4.966) Pub Date : 2021-01-09
    Jia Liu; MengLu Li; LingHao Kong; MengWen Cao; MoLan Zhang; YanHong Wang; ChengChuang Song; XingTang Fang; Hong Chen; ChunLei Zhang

    The noncoding RNAs play important role in growth and development of mammalian skeletal muscle. Recent work has shown that circRNAs are abundant in skeletal muscle tissue, with significant changes in their expression patterns during muscle development and aging. We identified a novel circRNA called circARID1A that is highly expressed in mice skeletal muscle compare to its linear transcript. Experiments

    更新日期:2021-01-10
  • Lipocalin‐2 mediates the rejection of neural transplants
    FASEB J. (IF 4.966) Pub Date : 2021-01-09
    Yi‐Chinn Weng; Yu‐Ting Huang; I‐Chen Chiang; Pei‐Ju Tsai; Yu‐Wen Su; Wen‐Hai Chou

    Lipocalin‐2 (LCN2) has been implicated in promoting apoptosis and neuroinflammation in neurological disorders; however, its role in neural transplantation remains unknown. In this study, we cultured and differentiated Lund human mesencephalic (LUHMES) cells into human dopaminergic‐like neurons and found that LCN2 mRNA was progressively induced in mouse brain after the intrastriatal transplantation

    更新日期:2021-01-10
  • Deficiency of circadian gene cryptochromes in bone marrow‐derived cells protects against atherosclerosis in LDLR−/− mice
    FASEB J. (IF 4.966) Pub Date : 2021-01-10
    Yu‐Sheng Lin; Ming‐Lung Tsai; I‐Chang Hsieh; Ming‐Shien Wen; Chao‐Yung Wang

    Cryptochromes are photoreceptors that mediate the circadian entrainment by light in plants and animals. They are also involved in magnetic field sensing in some animals. Recent studies suggest that cryptochromes play an essential role in metabolism and cardiovascular disease. However, the tissue‐specific function of cryptochromes in atherosclerosis is unknown. We transplanted bone marrow from wild‐type

    更新日期:2021-01-10
  • Ceramide synthase 2‐C24:1‐ceramide axis limits the metastatic potential of ovarian cancer cells
    FASEB J. (IF 4.966) Pub Date : 2021-01-10
    Xuewei Zhang; Wataru Sakamoto; Daniel Canals; Masumi Ishibashi; Masaya Matsuda; Kentaro Nishida; Masafumi Toyoshima; Shogo Shigeta; Makoto Taniguchi; Can E. Senkal; Toshiro Okazaki; Nobuo Yaegashi; Yusuf A. Hannun; Takeshi Nabe; Kazuyuki Kitatani

    Regulation of sphingolipid metabolism plays a role in cellular homeostasis, and dysregulation of these pathways is involved in cancer progression. Previously, our reports identified ceramide as an anti‐metastatic lipid. In the present study, we investigated the biochemical alterations in ceramide‐centered metabolism of sphingolipids that were associated with metastatic potential. We established metastasis‐prone

    更新日期:2021-01-10
  • Mss51 deletion increases endurance and ameliorates histopathology in the mdx mouse model of Duchenne muscular dystrophy
    FASEB J. (IF 4.966) Pub Date : 2021-01-10
    Yazmin I. Rovira Gonzalez; Adam L. Moyer; Nicolas J. LeTexier; August D. Bratti; Siyuan Feng; Vanessa Peña; Congshan Sun; Hannah Pulcastro; Ting Liu; Shama R. Iyer; Richard M. Lovering; Brian O'Rourke; Kathryn R. Wagner

    Mitochondrial derangement is an important contributor to the pathophysiology of muscular dystrophies and may be among the earliest cellular deficits. We have previously shown that disruption of Mss51, a mammalian skeletal muscle protein that localizes to the mitochondria, results in enhanced muscle oxygen consumption rate, increased endurance capacity, and improved limb muscle strength in mice with

    更新日期:2021-01-10
  • Protective role of IL33 signaling in negative pregnancy outcomes associated with lipopolysaccharide exposure
    FASEB J. (IF 4.966) Pub Date : 2021-01-10
    Keisuke Kozai; Khursheed Iqbal; Ayelen Moreno‐Irusta; Regan L. Scott; Mikaela E. Simon; Pramod Dhakal; Patrick E. Fields; Michael J. Soares

    Interleukin 33 (IL33) signaling has been implicated in the establishment and maintenance of pregnancy and in pregnancy disorders. The goal of this project was to evaluate the role of IL33 signaling in rat pregnancy. The rat possesses hemochorial placentation with deep intrauterine trophoblast invasion; features also characteristic of human placentation. We generated and characterized a germline mutant

    更新日期:2021-01-10
  • Regulation of the nuclear speckle localization and function of Rac1
    FASEB J. (IF 4.966) Pub Date : 2021-01-08
    Abdalla Abdrabou; Zhixiang Wang

    Despite significant evidence that Rac1 is localized to the nucleus, little is known regarding the function and biological significance of nuclear Rac1. Here, we showed that in response to EGF Rac1 was translocated to nuclear speckles and co‐localized with the nuclear speckle marker Serine/arginine‐rich splicing factor 2 (SRSF2) in Cos‐7 cells. We also showed that the nuclear speckle localization of

    更新日期:2021-01-08
  • Silencing of circIgf1r plays a protective role in neuronal injury via regulating astrocyte polarization during epilepsy
    FASEB J. (IF 4.966) Pub Date : 2021-01-08
    Lin Shao; Guang‐tong Jiang; Xing‐liang Yang; Meng‐liu Zeng; Jing‐jing Cheng; Shuo Kong; Xin Dong; Tao‐xiang Chen; Song Han; Jun Yin; Wan‐hong Liu; Xiao‐hua He; Chunjiang He; Bi‐wen Peng

    Epilepsy is a common brain disorder, repeated seizures of epilepsy may lead to a series of brain pathological changes such as neuronal or glial damage. However, whether circular RNAs are involved in neuronal injury during epilepsy is not fully understood. Here, we screened circIgf1r in the status epilepticus model through circRNA sequencing, and found that it was upregulated after the status epilepticus

    更新日期:2021-01-08
  • Multidrug resistance‐associated protein 4 (Mrp4) is a novel genetic factor in the pathogenesis of obesity and diabetes
    FASEB J. (IF 4.966) Pub Date : 2021-01-08
    Ajay C. Donepudi; Yoojin Lee; Ji‐Young Lee; John D. Schuetz; José E. Manautou

    Multidrug resistance protein 4 (Mrp4) is an efflux transporter known to transport several xenobiotics and endogenous molecules. We recently identified that the lack of Mrp4 increases adipose tissue and body weights in mice. However, the role of Mrp4 in adipose tissue physiology are unknown. The current study aimed at characterizing these specific roles of Mrp4 using wild‐type (WT) and knockout (Mrp4−/−)

    更新日期:2021-01-08
  • Endothelial cell secreted metalloproteinase‐2 enhances neural stem cell N‐cadherin expression, clustering, and migration
    FASEB J. (IF 4.966) Pub Date : 2021-01-08
    Rita Matta; Muhammad Sulaiman Yousafzai; Michael Murrell; Anjelica L. Gonzalez

    Neuroblasts have a clustered phenotype critical for their unidirectional migration, which in part is dependent on signaling from microvascular endothelial cells (EC) and pericytes (PC). Diffusible signals secreted by vascular cells have been demonstrated to increase survival, proliferation, and differentiation of subventricular zone resident neural stem cells (NSC); however, the signals that promote

    更新日期:2021-01-08
  • A general strategy to inhibit serine protease by targeting its autolysis loop
    FASEB J. (IF 4.966) Pub Date : 2021-01-08
    Longguang Jiang; Cai Yuan; Mingdong Huang

    Serine proteases are a large family of enzymes critical for multiple physiological processes, and proven diagnostic and therapeutic targets in several clinical indications. The high similarity of active sites among different serine proteases posts a challenge to reach high selectivity for inhibitors of serine proteases targeting at the active site. Here, we demonstrated that one particular surface

    更新日期:2021-01-08
  • Antagonistic effects of finerenone and spironolactone on the aldosterone‐regulated transcriptome of human kidney cells
    FASEB J. (IF 4.966) Pub Date : 2021-01-08
    Florian Le Billan; Julie Perrot; Elena Carceller; Simon Travers; Say Viengchareun; Peter Kolkhof; Marc Lombès; Jérôme Fagart

    Aldosterone, the main mineralocorticoid hormone in humans, plays a pivotal role in the control of water and salt reabsorption via activation of the mineralocorticoid receptor (MR). Alterations in MR signaling pathway lead to renal dysfunction, including chronic kidney disease and renal fibrosis, that can be prevented or treated with mineralocorticoid receptor antagonists (MRAs). Here, we used RNA‐Sequencing

    更新日期:2021-01-08
  • Myelination defects in the medial prefrontal cortex of Fkbp5 knockout mice
    FASEB J. (IF 4.966) Pub Date : 2021-01-06
    Koeul Choi; Joonhee Lee; Hyo Jung Kang

    The hypothalamic‐pituitary‐adrenal (HPA) axis plays a principal role in stress response regulation and has been implicated in the etiology of stress‐related disorders. The HPA axis regulates the normal synthesis and release of glucocorticoids; dysregulation of the HPA axis causes abnormal responses to stress. FK506‐binding protein 5 (FKBP5), a co‐chaperone of heat shock protein 90 in the glucocorticoid

    更新日期:2021-01-07
  • “Editor‐in‐relief”
    FASEB J. (IF 4.966) Pub Date : 2020-12-31
    Thoru Pederson

    This playful title is not only to rhyme with my actual one but also to convey a look back as well as forward, as this marks the first month after completing my initial 5‐year term as Editor‐in‐Chief. I am buoyed (and “relieved”) to have been given the privilege to continue and thank FASEB for wanting me to do so. We are now publishing more than twice the volume of papers as when I took over (451 in

    更新日期:2020-12-31
  • Signal transducer and transcriptional activation 1 protects against pressure overload‐induced cardiac hypertrophy
    FASEB J. (IF 4.966) Pub Date : 2020-12-29
    Changlin Zhen; Hongxia Liu; Li Gao; Yuanyuan Tong; Chaoyong He

    Signal transducers and transcriptional activation 1 (Stat1) is a member of the STATs family, and its role in various biological responses, including cell proliferation, differentiation, migration, apoptosis, and immune regulation has been extensively studied. We aimed to investigate its role in pathological cardiac hypertrophy, which is currently poorly understood. Experiments using H9C2 cardiomyocytes

    更新日期:2020-12-30
  • Myosin 18Aα targets the guanine nucleotide exchange factor β‐Pix to the dendritic spines of cerebellar Purkinje neurons and promotes spine maturation
    FASEB J. (IF 4.966) Pub Date : 2020-12-30
    Christopher J. Alexander; Melanie Barzik; Ikuko Fujiwara; Kirsten Remmert; Ya‐Xian Wang; Ronald S. Petralia; Thomas B. Friedman; John A. Hammer

    Myosin 18Aα is a myosin 2‐like protein containing unique N‐ and C‐terminal protein interaction domains that co‐assembles with myosin 2. One protein known to bind to myosin 18Aα is β‐Pix, a guanine nucleotide exchange factor (GEF) for Rac1 and Cdc42 that has been shown to promote dendritic spine maturation by activating the assembly of actin and myosin filaments in spines. Here, we show that myosin

    更新日期:2020-12-30
  • Intestinal epithelial tight junctions and permeability can be rescued through the regulation of endoplasmic reticulum stress by amniotic fluid stem cells during necrotizing enterocolitis
    FASEB J. (IF 4.966) Pub Date : 2020-12-29
    Bo Li; Carol Lee; Sinobol Chuslip; Dorothy Lee; George Biouss; Richard Wu; Yuhki Koike; Hiromu Miyake; Wan Ip; Tanja Gonska; Agostino Pierro

    Necrotizing enterocolitis (NEC) is one of the most severe gastrointestinal diseases affecting premature infants. It has been shown that NEC is associated with disrupted intestinal barrier and dysregulated endoplasmic reticulum (ER)‐stress response. It has also been shown that stem cells derived from amniotic fluid (AFSC) rescued intestinal injury in experimental NEC. Herein, we hypothesized that the

    更新日期:2020-12-29
  • Structures of the β‐barrel assembly machine recognizing outer membrane protein substrates
    FASEB J. (IF 4.966) Pub Date : 2020-12-28
    Le Xiao; Long Han; Bufan Li; Manfeng Zhang; Haizhen Zhou; Qingshan Luo; Xinzheng Zhang; Yihua Huang

    β‐barrel outer membrane proteins (β‐OMPs) play critical roles in nutrition acquisition, protein import/export, and other fundamental biological processes. The assembly of β‐OMPs in Gram‐negative bacteria is mediated by the β‐barrel assembly machinery (BAM) complex, yet its precise mechanism remains elusive. Here, we report two structures of the BAM complex in detergents and in nanodisks, and two crystal

    更新日期:2020-12-28
  • Spatial and temporal roles of SARS‐CoV PLpro—A snapshot
    FASEB J. (IF 4.966) Pub Date : 2020-12-28
    Shaomin Yan; Guang Wu

    SARS‐CoV and SARS‐CoV‐2 encode four structural and accessory proteins (spike, envelope, membrane and nucleocapsid proteins) and two polyproteins (pp1a and pp1ab). The polyproteins are further cleaved by 3C‐like cysteine protease (3CLpro) and papain‐like protease (PLpro) into 16 nonstructural proteins (nsps). PLpro is released from nsp3 through autocleavage, and then it cleaves the sites between nsp1/2

    更新日期:2020-12-28