c‐Jun N‐terminal kinases (JNKs) are constitutively activated in mammalian brains and are indispensable for their development and neural functions. MKK7 is an upstream activator of all JNKs. However, whether the common JNK signaling pathway regulates the brain’s control of social behavior remains unclear. Here, we show that female mice in which Mkk7 is deleted specifically in mature neurons (Mkk7flox/floxSyn‐Cre

Autophagy is an intracellular degradation system widely conserved among various species. Autophagy is induced by the depletion of various nutrients, and this degradation mechanism is essential for adaptation to such conditions. In this study, we demonstrated that sulfur depletion induces autophagy in the fission yeast Schizosaccharomyces pombe. Based on the finding that autophagy induced by sulfur

Mature adipocyte‐derived dedifferentiated fat (DFAT) cells have been identified to possess similar multipotency to mesenchymal stem cells, but a method for converting DFAT cells into hepatocytes was previously unknown. Here, using comprehensive analysis of gene expression profiles, we have extracted three transcription factors, namely, Foxa2, Hnf4a, and Sall1 (FHS), that can convert DFAT cells into

A proper balance between proliferation and differentiation of cerebellar granule cell precursors (GCPs) is required for appropriate cerebellar morphogenesis. The Skp1‐Cullin1‐F‐box (SCF) complex, an E3‐ubiquitin ligase complex, is involved in poly‐ubiquitination and subsequent degradation of various cell cycle regulators and transcription factors. However, it remains unknown how the SCF complex affects

(Pro)renin receptor ((P)RR)/ ATP6AP2 (ATPase, H+ transporting, lysosomal accessory protein 2) functions as an essential accessory subunit of vacuolar H+‐ATPase (V‐ATPase). V‐ATPase is necessary for lysosome function and autophagy. Autophagy is related to cell proliferation, migration, and invasion of various cancer cells. In this study, we aim to clarify the relationship between (P)RR and autophagy

Loss of mxc gene function in mature hemocytes of Drosophila mxcmbn1 mutant results in malignant hyperplasia in larval hematopoietic tissues termed lymph glands (LGs) owing to over‐proliferation of immature cells. This is a useful model for genetic analyses of leukemia progression. To identify other mutations that deteriorate the hyperplasia, we aimed to investigate whether hyper‐activation of common

Zebrafish is a useful model to study vertebrate hematopoiesis, but lack of antibodies to zebrafish proteins has limited purification of hematopoietic cells. Here, we purified neutrophils from larval and adult zebrafish using the lectin Phaseolus vulgaris erythroagglutinin (PHA‐E) and DRAQ5, a DNA‐staining fluorescent dye. In adult kidney marrow, we purified neutrophil‐like PHA‐E4low DRAQ5low cells

Ohnishi, T., Shirane, M., Hashimoto, Y., Saita, S., & Nakayama, K. (2014) Identification and characterization of a neuron‐specific isoform of protrudin. Genes to Cells. 19, 97–111. In Experimental procedures, the first sentence under the sub‐heading “Construction of expression plasmids” should read as follows: Construction of expression plasmids Mouse cDNAs encoding protrudin‐S or protrudin‐L (Gen‐Bank

Han, Y., Nakayama, J., Hayashi, Y., Jeong, S., Futakuchi, M., Ito, E., … Semba, K. (2020) Establishment and characterization of highly osteolytic luminal breast cancer cell lines by intracaudal arterial injection. Genes to Cells, 25, 111‐123. https://doi.org/10.1111/gtc.12743 The authors would like to draw the reader’s attention to the change in the email address of the corresponding author. The new