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What Actin and Myosin Do in the Nucleus: New Functions of the Well-Known Proteins Mol. Biol. (IF 1.2) Pub Date : 2024-03-17 A. A. Saidova, I. A. Vorobjev
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Verification of CRISPR/Cas9 Activity In Vitro via SSA-Based Dual-Luciferase Reporter System Mol. Biol. (IF 1.2) Pub Date : 2024-03-17
Abstract The CRISPR/Cas9 technique has emerged as a powerful and promising tool for precise genomic integration, which applied to various cell types and organisms, but its efficiency largely depends on single-guide RNA (sgRNA). There are multiple strategies available to evaluate the cleavage activity of sgRNAs, and one such approach is T7 endonuclease I (T7EI) assay, which is laborious and time consuming
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Promoter Mutation of the bZIP Transcription Factor BdABF Accelerates Flowering in Brachypodium distachyon Mol. Biol. (IF 1.2) Pub Date : 2024-03-17 B. J. Han, Z. J. Liu, W. M. Bai, F. T. Sun, Y. F. Niu, T. Y. Song, W. J. You, P. Y. Wang, N. Li, P. S. Su
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Application of Cadherin cRNA Probes in Brains of Alzheimer’s Disease Mouse Model Mol. Biol. (IF 1.2) Pub Date : 2024-03-17 H. Zhou, S. J. Du, F. Gendi, H. Y. Li, J. Fu, C. Chang
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Revision of Functionally Relevant and Widely Expressed Long Non-Coding RNAs Mol. Biol. (IF 1.2) Pub Date : 2024-03-17 D. Konina, M. Skoblov
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Rapamycin Regulates Lipopolysaccharide-Induced Microglial Phagocytosis In Vitro Mol. Biol. (IF 1.2) Pub Date : 2024-03-17
Abstract Microglia phagocytosis plays an important role in the pathogenesis of neurodegeneration. Defects or dysfunction of microglia phagocytosis were observed in neurodegenerative diseases, with different targets and associated receptors influencing the microglia response. Moreover, non-canonical LC3-associated (microtubule-associated protein 1 light chain 3) phagocytosis was extensively studied
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Peptide Engineering Approach to Introduce an Improved Calcitonin Mutant Mol. Biol. (IF 1.2) Pub Date : 2024-03-17 M. Zarei, B. Abedini, A. Dehshahri, M. Negahdaripour
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Prime-Editing Methods and pegRNA Design Programs Mol. Biol. (IF 1.2) Pub Date : 2024-03-07 E. V. Mikhaylova, B. R. Kuluev, G. A. Gerashchenkov, D. A. Chemeris, R. R. Garafutdinov, A. R. Kuluev, An. K. Baymiev, Al. K. Baymiev, A. V. Chemeris
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Photochemical Processes of Cell DNA Damage by UV Radiation of Various Wavelengths: Biological Consequences Mol. Biol. (IF 1.2) Pub Date : 2024-03-07 G. Ya. Fraikin, N. S. Belenikina, A. B. Rubin
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Regulation of Retrotransposons in Drosophila melanogaster Somatic Tissues Mol. Biol. (IF 1.2) Pub Date : 2024-03-07 P. A. Milyaeva, I. V. Kukushkina, A. R. Lavrenov, I. V. Kuzmin, A. I. Kim, L. N. Nefedova
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The Proteome of Extracellular Membrane Vesicles from Bacillus pumilus 3-19 Mol. Biol. (IF 1.2) Pub Date : 2024-03-07 W. Kurdy, P. V. Zelenikhin, G. Yu. Yakovleva, M. N. Sinyagina, A. I. Kolpakov, O. N. Ilinskaya
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Relationships between Cxcl12, Tweak, Notch1, and Yap mRNA Expression Levels in Molecular Mechanisms of Liver Fibrogenesis Mol. Biol. (IF 1.2) Pub Date : 2024-03-07 E. I. Lebedeva, A. T. Shchastniy, A. S. Babenka, D. A. Zinovkin
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Genome Stability of Bacillus velezensis after Two-Year Exposure in Open Space Mol. Biol. (IF 1.2) Pub Date : 2024-03-07 S. V. Fialkina, E. A. Deshevaya, A. L. Rakitin, O. I. Orlov
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A Group of New Hypermethylated Long Non-Coding RNA Genes Associated with the Development and Progression of Breast Cancer Mol. Biol. (IF 1.2) Pub Date : 2024-03-07 E. A. Filippova, V. I. Loginov, S. S. Lukina, A. M. Burdennyy, I. V. Pronina, T. P. Kazubskaya, E. A. Braga
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Structure and Evolution of DNA Transposons of the L31 Superfamily in Bivalves Mol. Biol. (IF 1.2) Pub Date : 2024-03-07 M. V. Puzakov, L. V. Puzakova
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Melatonin Enhances the Effect of ABT-737 in Acute Monocytic Leukemia THP-1 Cells Mol. Biol. (IF 1.2) Pub Date : 2024-02-01
Abstract Melatonin (N-acetyl-5-methoxytryptamine, MEL) is a hormone synthesized by the pineal gland. Due to its oncostatic effect, it can be considered as an antitumor agent and used for combination therapy. ABT-737, a Bcl-2 inhibitor, promotes cell death after treatment with agents that induce pro-apoptotic signals. In the present study, the combined effect of MEL and ABT-737 on changes in proliferative
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Stochastic Packaging of Cas Proteins into Exosomes Mol. Biol. (IF 1.2) Pub Date : 2024-02-01
Abstract—CRISPR/Cas systems are perspective molecular tools for targeted manipulation with genetic materials, such as gene editing, regulation of gene transcription, modification of epigenome etc. While CRISPR/Cas systems proved to be highly effective for correcting genetic disorders and treating infectious diseases and cancers in experimental settings, clinical translation of these results is hampered
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Restraint Stress-Induced Expression of Fos and Several Related Genes in the Hypothalamus of Hypertensive ISIAH Rats Mol. Biol. (IF 1.2) Pub Date : 2024-02-01
Abstract Stress can play a significant role in arterial hypertension and many other complications of cardiovascular diseases. Considerable attention is paid to the study of the molecular mechanisms involved in the body response to stressful influences, but there are still many blank spots in understanding the details. ISIAH rats model the stress-sensitive form of arterial hypertension. ISIAH rats are
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Modeling Protein–Protein Interaction of the KNOTTED-LIKE HOMEOBOX 3 Protein Involved in Symbiotic Nodule Development in Medicago truncatula Mol. Biol. (IF 1.2) Pub Date : 2024-01-21 M. Azarakhsh, M. Lebedeva, V. K. Vishvakarma
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REEP3 as a Novel Oncogene Contributes to the Warburg Effect in Pancreatic Cancer Cells by Activating the EGFR/ERK Pathway Mol. Biol. (IF 1.2) Pub Date : 2024-01-21
Abstract Emerging evidence indicates that the Warburg effect (aerobic glycolysis) is a marker of malignancy in pancreatic cancer (PC). Receptor expression–enhancing protein 3 (REEP3) is dysregulated in various cancers; however, no studies have addressed whether REEP3 is involved in regulating PC malignancy, particularly with respect to the Warburg effect. Herein, we identified a new diagnostic marker
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Angiotensin II Type I Receptor—168A/G Polymorphism Is Associated with Increased the Risk of Glioma in Turkish Population Mol. Biol. (IF 1.2) Pub Date : 2024-01-21 T. Turan, B. Özaydın, Ö. H. Emmez, A. M. Kaymaz, İ. I. Gönül, M. Bozkurt, A. Gönenç
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Sestrin2 Alleviates Sepsis-Induced Renal Injury by Inhibiting NLRP3 Activation and Reactive Oxygen Species Production Mol. Biol. (IF 1.2) Pub Date : 2024-01-21
Abstract Sepsis is a life-threatening disorder that develops in response to an infection. Kidneys are one of the most common organs affected by sepsis. This study explored the influence of Sestrin2 regulating NLRP3 inflammasome activation on sepsis-induced renal injury. A sepsis mouse model was established by intraperitoneal injection of LPS. Adenoviral transduction was used to upregulate Sestrin2
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Heat Shock Proteins in Plant Protection from Oxidative Stress Mol. Biol. (IF 1.2) Pub Date : 2023-12-07 N. P. Yurina
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The Effects of the Hydrogen Sulfide Donor GYY4137 on the Proteasome Pool of Colorectal Cancer Cells Mol. Biol. (IF 1.2) Pub Date : 2023-12-07 E. V. Grigorieva, T. M. Astakhova, A. V. Burov, V. L. Karpov, A. V. Morozov
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Nitric Oxide(II) in the Biology of Chlorophyta Mol. Biol. (IF 1.2) Pub Date : 2023-12-07 E. V. Ermilova
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Changes in the Activity of Antioxidant Systems of Escherichia coli under Phosphate Starvation Mol. Biol. (IF 1.2) Pub Date : 2023-12-07 G. V. Smirnova, A. V. Tyulenev, N. G. Muzyka, L. V. Sutormina, O. N. Oktyabrsky
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The Antioxidant and Geroprotective Properties of an Extract of Mountain Ash (Sorbus aucuparia L.) Fruits Mol. Biol. (IF 1.2) Pub Date : 2023-12-07 E. Yu. Platonova, D. A. Golubev, N. V. Zemskaya, O. G. Shevchenko, S. A. Patov, M. V. Shaposhnikov, A. A. Moskalev
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Protein Repeats Show Clade-Specific Volatility in Aves Mol. Biol. (IF 1.2) Pub Date : 2023-12-07 S. Sharma, L. Teekas, N. Vijay
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Long Noncoding RNAs MEG3, TUG1, and hsa-miR-21-3p Are Potential Diagnostic Biomarkers for Coronary Artery Disease Mol. Biol. (IF 1.2) Pub Date : 2023-12-07 M. Abdelgawad, H. Y. Abdallah, A. Fareed, A. E. Ahmed
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Ischemia–Reperfusion Injury: Molecular Mechanisms of Pathogenesis and Methods of Their Correction Mol. Biol. (IF 1.2) Pub Date : 2023-12-07 R. G. Goncharov, M. G. Sharapov
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The Protective Action of Hsp70 and Hydrogen Sulfide Donors in THP-1 Macrophages in the Lipopolysaccharide-Induced Inflammatory Response by Modulating Endocytosis Mol. Biol. (IF 1.2) Pub Date : 2023-12-07 M. M. Yurinskaya, D. G. Garbuz, M. B. Evgen’ev, M. G. Vinokurov
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Characteristics of δ-Aminolevulinic Acid Dehydratase of the Cold-Water Sponge Halisarca dujardinii Mol. Biol. (IF 1.2) Pub Date : 2023-12-07 O. I. Kravchuk, A. D. Finoshin, K. V. Mikhailov, R. H. Ziganshin, K. I. Adameyko, N. G. Gornostaev, A. I. Zhurakovskaya, V. S. Mikhailov, E. I. Shagimardanova, Yu. V. Lyupina
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Metabolic Heterogeneity of Tumors Mol. Biol. (IF 1.2) Pub Date : 2023-12-07 M. V. Shirmanova, S. D. Sinyushkina, A. D. Komarova
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Interaction of SENP6 with PINK1 Promotes Temozolomide Resistance in Neuroglioma Cells via Inducing the Mitophagy Mol. Biol. (IF 1.2) Pub Date : 2023-12-07 Y. W. Wang, K. G. Jia, H. J. Xing, Y. Pan, C. S. Zeng, L. Chen, Q. J. Su, W. T. Shen, J. Chen, C. Chen, Q. Cao, Y. Y. Wang
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Metabolic Stress of Red Blood Cells Induces Hemoglobin Glutathionylation Mol. Biol. (IF 1.2) Pub Date : 2023-12-07 P. I. Zaripov, Yu. D. Kuleshova, Yu. M. Poluektov, S. V. Sidorenko, O. K. Kvan, G. V. Maksimov, V. A. Mitkevich, A. A. Makarov, I. Yu. Petrushanko
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Identification of Melittin-Like Proteins with a Molecular Weight of 67 kDa that Interact with Na+/K+-ATPase Mol. Biol. (IF 1.2) Pub Date : 2023-12-07 L. A. Varfolomeeva, E. A. Klimanova, S. V. Sidorenko, D. A. Fedorov, O. D. Lopina
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The Redox-Catalytic Properties of Cobalamins Mol. Biol. (IF 1.2) Pub Date : 2023-12-07 Yu. V. Shatalin, V. S. Shubina, M. E. Solovieva, V. S. Akatov
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The Thymic Hormone Thymosin-1α Reduces the Pro-Inflammatory Response of Raw 264.7 Cells Induced by Endotoxin Mol. Biol. (IF 1.2) Pub Date : 2023-12-07 E. G. Novoselova, O. V. Glushkova, M. O. Khrenov, S. M. Lunin, M. G. Sharapov, R. G. Goncharov, E. K. Mubarakshina, T. V. Novoselova, S. B. Parfenyuk
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Regulation of Metabolism and the Role of Redox Factors in the Energy Control of Quiescence and Proliferation of Hematopoietic Cells Mol. Biol. (IF 1.2) Pub Date : 2023-12-07 M. V. Kalashnikova, N. S. Polyakova, A. V. Belyavsky
Abstract—One of the key regulators of hematopoietic stem cell (HSC) maintenance is cellular metabolism. Resting HSCs use anaerobic glycolysis as the main source of energy. During expansion and differentiation under conditions of steady state hematopoiesis, the energy needs of activated HSCs increase by many fold. To meet the increased demands, cells switch to mitochondrial oxidative phosphorylation
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The Development of SpCas9 Variants with High Specificity and Efficiency Based on the HH Theory Mol. Biol. (IF 1.2) Pub Date : 2023-11-22 G. H. Wang, C. M. Wang, X. J. Wu, T. Chu, D. W. Huang, J. Li
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Analysis of the Complete Tomato Aspermy Virus Genomes Suggests Reassortment in Russian Isolates from Chrysanthemum Mol. Biol. (IF 1.2) Pub Date : 2023-10-09 A. A. Sheveleva, G. S. Krasnov, A. V. Kudryavtseva, A. V. Snezhkina, E. V. Bulavkina, S. N. Chirkov
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A Comparative Analysis of Mitochondrial Genome Mutation Spectra in Human Populations Mol. Biol. (IF 1.2) Pub Date : 2023-10-09 B. A. Malyarchuk
Abstract—Nucleotide sequence variability of whole mitochondrial genomes (mtDNA) was analyzed and mutation spectra were reconstructed (by L-chain of mtDNA) in four regional groups of indigenous populations representing Northeastern and Southern Siberia, Western Asia, and the Americas. The pyrimidine transitions were found to be predominant in all groups; of these, the T→C substitutions were most frequent
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The Role of the WGR Domain in the Functions of PARP1 and PARP2 Mol. Biol. (IF 1.2) Pub Date : 2023-10-09 N. V. Maluchenko, A. N. Korovina, A. A. Saulina, V. M. Studitsky, A. V. Feofanov
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Digital PCR as a Highly Sensitive Diagnostic Tool: A Review Mol. Biol. (IF 1.2) Pub Date : 2023-10-09 K. V. Kopylova, Ed. W. Kasparov, I. V. Marchenko, M. V. Smolnikova
Abstract—Digital PCR (dPCR) is a nucleic acid quantification method that is widely used in genetic analysis. One of the most significant advantages of dPCR over other methods is the possibility of absolute quantitative determination of genetic material without construction of calibration curves, which allows one to detect even single molecules of nucleic acids, and, hence, provides early diagnosis
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Cancer-Associated Fibroblasts: Heterogeneity and Bimodality in Oncogenesis Mol. Biol. (IF 1.2) Pub Date : 2023-10-09 N. A. Lunina, D. R. Safina, S. V. Kostrov
Abstract Cancer-associated fibroblasts (CAFs) often form a major component of the tumor microenvironment (TMA), providing conditions for cancer cells to thrive. CAFs may contribute to tumor growth, invasion, metastasis, and resistance to therapy. However, clinical trials of treatment strategies targeting CAFs have largely failed. Moreover, there is evidence that CAFs are capable of inhibiting tumor
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Association of Polymorphic Genome Variants in the 2q32.1 Locus with the Development of Vasovagal Syncope Mol. Biol. (IF 1.2) Pub Date : 2023-10-09 N. A. Matveeva, B. V. Titov, E. A. Bazyleva, E. A. Kuchinskaya, M. S. Kozin, A. V. Favorov, A. V. Pevzner, O. O. Favorova
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Efficiency of Escherichia coli and Bacillus subtilis Expression Systems for Production of Binase Mutants Mol. Biol. (IF 1.2) Pub Date : 2023-10-09 A. I. Nadyrova, A. S. Kosnyrev, V. V. Ulyanova, E. V. Dudkina, V. I. Vershinina, O. N. Ilinskaya
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Changes in the Activities and Contents of Individual Forms of Proteasomes in Samples of the Cerebral Cortex during Pathology Development in 5xFAD Mice Mol. Biol. (IF 1.2) Pub Date : 2023-10-09 A. V. Morozov, A. V. Burov, S. Yu. Funikov, E. V. Teterina, T. M. Astakhova, P. A. Erokhov, A. A. Ustyugov, V. L. Karpov
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Drosophila melanogaster Lifespan Is Regulated by nejire Gene Expression in Peripheral Tissues and Nervous System Mol. Biol. (IF 1.2) Pub Date : 2023-10-09 L. A. Koval, E. N. Proshkina, N. V. Zemskaya, I. A. Solovev, E. V. Schegoleva, M. V. Shaposhnikov, A. A. Moskalev
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The Mechanism of Bimodal Effect of DL-Butyonine Sulfoximine on Constitutive Androstane and Pregnane X Receptors In Vitro Mol. Biol. (IF 1.2) Pub Date : 2023-10-09 Y. V. Abalenikhina, A. V. Shchulkin, A. A. Seidkulieva, E. D. Rokunov, F. T. Gadzhieva, E. N. Yakusheva
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Dynamic Changes in the Activities and Contents of Particular Proteasome Forms in the Cerebral Cortex of C57BL/6 Mice during Aging Mol. Biol. (IF 1.2) Pub Date : 2023-10-09 A. V. Burov, S. Yu. Funikov, T. M. Astakhova, E. V. Teterina, V. O. Nebogatikov, P. A. Erokhov, A. A. Ustyugov, V. L. Karpov, A. V. Morozov
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Hepatitis C Virus Nonstructural Protein 3 Increases Secretion of Interleukin-1beta in HEK293T Cells with a Reconstructed NLRP3 Inflammasome Mol. Biol. (IF 1.2) Pub Date : 2023-10-09 A. A. Latanova, K. K. Tuchinskaya, E. S. Starodubova, V. L. Karpov
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Cytochrome bd as Antioxidant Redox Enzyme Mol. Biol. (IF 1.2) Pub Date : 2023-09-06 V. B. Borisov, M. R. Nastasi, E. Forte
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Alternative Mechanisms of Mutagenesis at mCpG Sites during Replication and Repair Mol. Biol. (IF 1.2) Pub Date : 2023-08-08 E. S. Shilkin, D. V. Petrova, D. O. Zharkov, A. V. Makarova
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Identification of Clinical Isolates of the Bacillus cereus Group and Their Characterization by Mass Spectrometry and Electron Microscopy Mol. Biol. (IF 1.2) Pub Date : 2023-08-08 T. A. Smirnova, N. B. Polyakov, D. S. Karpov, A. I. Solovyev, N. V. Shevlyagina, S. G. Andreevskaya, D. N. Shcherbinin, Z. S. Plieva, V. A. Kozlova, A. A. Pereborova, I. A. Bogdanov, D. A. Grumov, M. V. Zubasheva, S. V. Poddubko, A. A. Grechnikov, M. A. Sukhina, V. G. Zhukhovitsky
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DNA Phenotyping of Remains from Elite Burials of the Khazar Period of Southern Russia Mol. Biol. (IF 1.2) Pub Date : 2023-08-08 D. O. Fesenko, O. Yu. Aramova, E. V. Vdovchenkov, I. D. Ivanovsky, O. E. Fesenko, S. A. Polyakov, T. G. Faleeva, M. A. Filippova, V. S. Florinskaya, I. V. Kornienko
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Molecular and Cellular Aspects of the Endothelial–Mesenchymal Transition in Cardiovascular Diseases Mol. Biol. (IF 1.2) Pub Date : 2023-08-08 E. A. Strelnikova, R. E. Kalinin, I. A. Suchkov, N. V. Korotkova, N. D. Mzhavanadze
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Universal Panel of Insertion/Deletion Polymorphisms and Biochip-Based Kit ChipID106 for Genetic Personal Identification Mol. Biol. (IF 1.2) Pub Date : 2023-08-08 D. O. Fesenko, I. D. Ivanovsky, P. L. Ivanov, E. Yu. Zemskova, S. A. Polyakov, O. E. Fesenko, M. A. Filippova, A. S. Zasedatelev
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Assembly and Disassembly of the Nuclear Pore Complex: A View from the Structural Side Mol. Biol. (IF 1.2) Pub Date : 2023-08-08 A. V. Orlova, S. G. Georgieva, D. V. Kopytova