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Development of an in vitro human alveolar epithelial air-liquid interface model using a small molecule inhibitor cocktail BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2024-03-18 Ikuya Tanabe, Kanae Ishimori, Shinkichi Ishikawa
The alveolar epithelium is exposed to numerous stimuli, such as chemicals, viruses, and bacteria that cause a variety of pulmonary diseases through inhalation. Alveolar epithelial cells (AECs) cultured in vitro are a valuable tool for studying the impacts of these stimuli and developing therapies for associated diseases. However, maintaining the proliferative capacity of AECs in vitro is challenging
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Mechanical stretch leads to increased caveolin-1 content and mineralization potential in extracellular vesicles from vascular smooth muscle cells BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2024-03-14 Mohammad Shaver, Kassandra Gomez, Katherine Kaiser, Joshua D. Hutcheson
Hypertension-induced mechanical stress on vascular smooth muscle cells (VSMCs) is a known risk factor for vascular remodeling, including vascular calcification. Caveolin-1 (Cav-1), an integral structural component of plasma membrane invaginations, is a mechanosensitive protein that is required for the formation of calcifying extracellular vesicles (EVs). However, the role of mechanics in Cav-1-induced
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Melatonin reduces lung injury in type 1 diabetic mice by the modulation of autophagy BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2024-03-14 Jafar Rezaie, Mojtaba Jahanghiri, Reza Mosaddeghi- Heris, Sina Hassannezhad, Nima Abdyazdani, Afshin Rahbarghazi, Mahdi Ahmadi
In recent years, the role of autophagy has been highlighted in the pathogenesis of diabetes and inflammatory lung diseases. In this study, using a diabetic model of mice, we investigated the expression of autophagy-related genes in the lung tissues following melatonin administration. Data showed histopathological remodeling in lung tissues of the D group coincided with an elevated level of IL-6, Becline-1
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TonEBP/NFAT5 expression is associated with cisplatin resistance and migration in macrophage-induced A549 cells BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2024-03-04 Hee Ju Song, Young Hwan Kim, Han Na Choi, Taehee Kim, Soo Jin Kim, Min Woong Kang, Sang Do Lee
Macrophages promote angiogenesis, metastasis, and drug resistance in several cancers. Similarly, TonEBP/NFAT5 induces metastasis in renal carcinoma and colon cancer cells. However, the role of this transcription factor and that of macrophages in lung cancer cells remains unclear. Therefore, this study investigated the effects of macrophages and TonEBP/NFAT5 expression on cisplatin resistance and migration
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Optimizing combination therapy in prostate cancer: mechanistic insights into the synergistic effects of Paclitaxel and Sulforaphane-induced apoptosis BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2024-03-04 Tito N. Habib, Mohammed O. Altonsy, Salah A. Ghanem, Mohamed S. Salama, Mai A. Hosny
Combination therapies in cancer treatment have demonstrated synergistic or additive outcomes while also reducing the development of drug resistance compared to monotherapy. This study explores the potential of combining the chemotherapeutic agent Paclitaxel (PTX) with Sulforaphane (SFN), a natural compound primarily found in cruciferous vegetables, to enhance treatment efficacy in prostate cancer.
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CTC together with Shh and Nrf2 are prospective diagnostic markers for HNSCC BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2024-02-10 Md. Mizanur Rahman, Muhammad Mosaraf Hossain, Shafiqul Islam, Ridwan Ahmed, Mohit Majumder, Shantu Dey, Md. Kawser, Bishu Sarkar, Md. Ejajur Rahman Himu, Ali Asgar Chowdhury, Shakera Ahmed, Supran Biswas, Mostafa Mahfuzul Anwar, Mohammad Jamal Hussain, Rajib Kumar Shil, Sunanda Baidya, Ramendu Parial, Mohammed Moinul Islam, Atul Bharde, Sreeja Jayant, Gourishankar Aland, Jayant Khandare, Shaikh Bokhtear
The lack of appropriate prognostic biomarkers remains a significant obstacle in the early detection of Head and Neck Squamous Cell Carcinoma (HNSCC), a cancer type with a high mortality rate. Despite considerable advancements in treatment, the success in diagnosing HNSCC at an early stage still needs to be improved. Nuclear factor erythroid 2-related factor 2 (Nrf2) and Sonic Hedgehog (Shh) are overexpressed
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Prioritization of Trypanosoma brucei editosome protein interactions interfaces at residue resolution through proteome-scale network analysis BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2024-01-26 Naghmeh Poorinmohammad, Reza Salavati
Trypanosoma brucei is the causative agent for trypanosomiasis in humans and livestock, which presents a growing challenge due to drug resistance. While identifying novel drug targets is vital, the process is delayed due to a lack of functional information on many of the pathogen’s proteins. Accordingly, this paper presents a computational framework for prioritizing drug targets within the editosome
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Sumoylation of SAP130 regulates its interaction with FAF1 as well as its protein stability and transcriptional repressor function BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2024-01-04 Chang-Han Chen, Hung-Wei Lin, Meng-Fang Huang, Chi-Wu Chiang, Kuen-Haur Lee, Nguyen Thanh Phuong, Zong-Yan Cai, Wen-Chang Chang, Ding-Yen Lin
Fas-associated factor 1 (FAF1) is a multidomain protein that interacts with diverse partners to affect numerous cellular processes. Previously, we discovered two Small Ubiquitin-like Modifier (SUMO)-interacting motifs (SIMs) within FAF1 that are crucial for transcriptional modulation of mineralocorticoid receptor. Recently, we identified Sin3A-associated protein 130 (SAP130), a putative sumoylated
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Loss of Dec1 inhibits alcohol-induced hepatic lipid accumulation and circadian rhythm disorder BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2024-01-02 Fuyuki Sato, Ujjal K. Bhawal, Kosuke Oikawa, Yasuteru Muragaki
Chronic alcohol exposure increases liver damage such as lipid accumulation and hepatitis, resulting in hepatic cirrhosis. Chronic alcohol intake is known to disturb circadian rhythms in humans and animals. DEC1, a basic helix-loop-helix transcription factor, plays an important role in the circadian rhythm, inflammation, immune responses, and tumor progression. We have previously shown that Dec1 deficiency
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Association between plasma L-carnitine levels and mitochondrial DNA copy number BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-12-11 Mingyue Li, Keming Yang, Immaculata De Vivo, A. Heather Eliassen, Abrar A. Qureshi, Hongmei Nan, Jiali Han
Mitochondria are key cytoplasmic organelles in eukaryotic cells that generate adenosine triphosphate (ATP) through the electron transport chain and oxidative phosphorylation. Mitochondrial DNA (mtDNA) copy number (mtDNAcn) is considered a biomarker for both mitochondrial quantity and function as well as cellular oxidative stress level. Previous epidemiologic findings revealed that weight gain, higher
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Effect of Emi1 gene silencing on the proliferation and invasion of human breast cancer cells BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-12-01 Ying Kuang, Shengwen Huang, Shifan Tang, Zhaozhen Zhuo, Keyan Linghu
Breast cancer is the most common malignant tumour in women. The early silk-splitting inhibitor protein 1 Emi1 is responsible for mediating ubiquitin protein degradation. The present study investigated the effects of the decreased expression of the Emil gene on the proliferation and invasion of breast cancer cells. The interference efficiency of small interfering ribonucleic acid (siRNA) was quantitatively
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TNFα induces Caspase-3 activity in hematopoietic progenitor cells CD34+, CD33+, and CD41 + of myelodysplastic syndromes BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-11-21 Anggraini Iriani, Andhika Rachman, Rahayuningsih D. Setiabudy, Siti B. Kresno, Aru W. Sudoyo, Mansyur Arief, Alida R. Harahap, Marsya Kaila Fatina
Cytopenia is the primary feature of Myelodysplastic Syndrome, even in the presence of hypercellular bone marrow. TNFα is recognized as both a proinflammatory, and proapoptotic cytokine with a well established role in promoting apoptosis in MDS. Therefore, TNFα has the potential to be a valuable biomarker for predicting the progression of cytopenia in MDS. This study aims to establish the role of TNFα
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From network analysis to experimental validation: identification of regulators of non-muscle myosin II contractility using the folded-gastrulation signaling pathway BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-10-11 Andy Zhao, Sophia Varady, Madelyn O’Kelley-Bangsberg, Vicki Deng, Amy Platenkamp, Petra Wijngaard, Miriam Bern, Wyatt Gormley, Elaine Kushkowski, Kat Thompson, Logan Tibbetts, A. Tamar Conner, David Noeckel, Aidan Teran, Anna Ritz, Derek A. Applewhite
The morphogenetic process of apical constriction, which relies on non-muscle myosin II (NMII) generated constriction of apical domains of epithelial cells, is key to the development of complex cellular patterns. Apical constriction occurs in almost all multicellular organisms, but one of the most well-characterized systems is the Folded-gastrulation (Fog)-induced apical constriction that occurs in
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Simple, low-cost, and well-performing method, the outgrowth technique, for the isolation of cells from nasal polyps BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-10-10 Jonghui Kim, Karla Hegener, Claudia Hagedorn, Daniel Weidinger, Kashin Jamal Jameel, Inga Marte Charlott Seuthe, Sabine Eichhorn, Florian Kreppel, Jonas Jae-Hyun Park, Jürgen Knobloch
Epithelial cells are an important part of the pathomechanism in chronic rhinosinusitis with nasal polyps. It is therefore essential to establish a robust method for the isolation and culture of epithelial cells from nasal polyps to enable further research. In this study, the feasibility of the outgrowth technique for the isolation of the epithelial cells from the nasal polyps was evaluated. Using the
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Comprehensive brain tissue metabolomics and biological network technology to decipher the mechanism of hydrogen-rich water on Radiation-induced cognitive impairment in rats BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-09-26 Xiaoming Liu, Mengya Liu, Huan Liu, Hui Yuan, Yong Wang, Xiaoman Chen, Jianguo Li, Xiujun Qin
Hydrogen-rich water (HRW) has been shown to prevent cognitive impairment caused by ionizing radiation. This study aimed to investigate the pharmacological effects and mechanisms of HRW on ionizing radiation by coupling the brain metabolomics and biological target network methods. HRW significantly improves the cognitive impairment in rats exposed to ionizing radiation. Based on metabolomics and biological
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Mineral elements and adiposity-related consequences in adolescents with intellectual disabilities BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-09-20 Ahmad H. Alghadir, Sami A. Gabr, Amir Iqbal
Patients with intellectual disabilities are shown to have a limited capacity for cooperation, communication,and other biological consequences, which significantly require a specialized interest in healthcare professionals worldwide. In this respect, the present study was designed to evaluate the levels mineral elements, and their correlation with oxidative stress markers and adiposity markers; leptin
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Glycyrrhizin inhibits LPS-induced inflammatory responses in goat ruminal epithelial cells in vitro BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-09-19 Junfeng Liu, Bei Ma, Guang Hao, DuoDuo Su, Tianyang Wang, Ze Ding, Xuefeng Guo
Inflammation plays a crucial role in the progression of Subacute Ruminal Acidosis (SARA). The experiment was designed to investigate anti-inflammatory effects of glycyrrhizin on goats ruminal epithelial cells (GREC) which were induced SARA by Lipopolysaccharide (LPS) in vitro. The GREC were induced SARA by adding LPS at the concentration of 5 μm and glycyrrhizin was added at different concentration
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D-galactose-induced mitochondrial oxidative damage and apoptosis in the cochlear stria vascularis of mice BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-08-21 Zhe Peng, Chunli Zhao, Zijing Yang, Shusheng Gong, Zhengde Du
Age-related hearing loss, known as presbycusis, is the result of auditory system degeneration. Numerous studies have suggested that reactive oxygen species (ROS) and mitochondrial oxidative damage play important roles in the occurrence and progression of aging. The D-galactose (D-gal)-induced aging model is well known and widely utilized in aging research. Our previous studies demonstrate that administration
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Keratin 19 binds and regulates cytoplasmic HNRNPK mRNA targets in triple-negative breast cancer BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-08-17 Arwa Fallatah, Dimitrios G. Anastasakis, Amirhossein Manzourolajdad, Pooja Sharma, Xiantao Wang, Alexis Jacob, Sarah Alsharif, Ahmed Elgerbi, Pierre A. Coulombe, Markus Hafner, Byung Min Chung
Heterogeneous nuclear ribonucleoprotein K (HNRNPK) regulates pre-mRNA processing and long non-coding RNA localization in the nucleus. It was previously shown that shuttling of HNRNPK to the cytoplasm promotes cell proliferation and cancer metastasis. However, the mechanism of HNRNPK cytoplasmic localization, its cytoplasmic RNA ligands, and impact on post-transcriptional gene regulation remain uncharacterized
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A computational peptide model induces cancer cells’ apoptosis by docking Kringle 5 to GRP78 BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-08-08 Ibrahim Khater, Aaya Nassar
Cells can die through a process called apoptosis in both pathological and healthy conditions. Cancer development and progression may result from abnormal apoptosis. The 78-kDa glucose-regulated protein (GRP78) is increased on the surface of cancer cells. Kringle 5, a cell apoptosis agent, is bound to GRP78 to induce cancer cell apoptosis. Kringle 5 was docked to GRP78 using ClusPro 2.0. The interaction
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BMP9 maintains the phenotype of HTR-8/Svneo trophoblast cells by activating the SDF1/CXCR4 pathway BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-08-07 Xue Yang, Lingling Ren, Xiang Chen, Ying Pang, Baoxia Jia, Jing Sun, Xiaofang Quan
Bone morphogenetic protein 9 (BMP9) has been shown to regulate processes such as angiogenesis, endothelial dysfunction, and tumorigenesis. However, the role of BMP9 in preeclampsia (PE) is unclear. The purpose of this study was to investigate the role and mechanism of BMP9 in PE. The effects of BMP9 on the viability, migration and invasion of HTR-8/Svneo cells were investigated by CCK-8 assay, wound
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Emodin and aloe-emodin, two potential molecules in regulating cell migration of skin cells through the MAP kinase pathway and affecting Caenorhabditis elegans thermotolerance BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-07-25 Aysenur Gunaydin-Akyildiz, Rabia Sare Yanikoglu, Meltem Gulec, Gulbahar Ozge Alim-Toraman, Ebru Didem Kuran, Sezen Atasoy, Abdullah Olgun, Gulacti Topcu
Emodin and aloe-emodin are two anthraquinones having positive effects in wound healing. However, their mechanism of action of wound healing is not fully understood. The MAP kinase family, which plays an active role in wound healing, is a well-characterized large family of serine/threonine kinases and regulates processes such as proliferation, oncogenesis, differentiation, and inflammation in the cell
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Knockdown of ELF4 aggravates renal injury in ischemia/reperfusion mice through promotion of pyroptosis, inflammation, oxidative stress, and endoplasmic reticulum stress BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-07-20 Li Li, Shunying Wang, Wenming Wang
Renal ischemia/reperfusion (I/R) injury is a major cause of acute kidney injury (AKI). Dysfunction of E74-like ETS transcription factor 4 (ELF4) leads to inflammation. This research intended to look into the function and mechanisms of ELF4 in I/R and oxygen–glucose deprivation/reperfusion (OGD/R) model. In I/R and OGD/R model, ELF4 expression was downregulated. ELF4 knockout aggravated I/R-induced
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Janus Kinase 3 phosphorylation and the JAK/STAT pathway are positively modulated by follicle-stimulating hormone (FSH) in bovine granulosa cells BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-06-20 Amir Zareifard, Francis Beaudry, Kalidou Ndiaye
Janus kinase 3 (JAK3) is a member of the JAK family of tyrosine kinase proteins involved in cytokine receptor-mediated intracellular signal transduction through the JAK/STAT signaling pathway. JAK3 was previously shown as differentially expressed in granulosa cells (GC) of bovine pre-ovulatory follicles suggesting that JAK3 could modulate GC function and activation/inhibition of downstream targets
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Genetic and protein interaction studies between the ciliary dyslexia candidate genes DYX1C1 and DCDC2 BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-05-26 Andrea Bieder, Gayathri Chandrasekar, Arpit Wason, Steffen Erkelenz, Jay Gopalakrishnan, Juha Kere, Isabel Tapia-Páez
DYX1C1 (DNAAF4) and DCDC2 are two of the most replicated dyslexia candidate genes in genetic studies. They both have demonstrated roles in neuronal migration, in cilia growth and function and they both are cytoskeletal interactors. In addition, they both have been characterized as ciliopathy genes. However, their exact molecular functions are still incompletely described. Based on these known roles
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SUMOylation of PDGF receptor α affects signaling via PLCγ and STAT3, and cell proliferation BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-05-16 Kehuan Wang, Natalia Papadopoulos, Anahita Hamidi, Johan Lennartsson, Carl-Henrik Heldin
The platelet-derived growth factor (PDGF) family of ligands exerts their cellular effects by binding to α- and β-tyrosine kinase receptors (PDGFRα and PDGFRβ, respectively). SUMOylation is an important posttranslational modification (PTM) which regulates protein stability, localization, activation and protein interactions. A mass spectrometry screen has demonstrated SUMOylation of PDGFRα. However,
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Myogenic differentiation of human myoblasts and Mesenchymal stromal cells under GDF11 on NPoly-ɛ-caprolactone-collagen I-Polyethylene-nanofibers BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-05-15 Aijia Cai, Paul Schneider, Zeng-Ming Zheng, Justus P. Beier, Marcus Himmler, Dirk W. Schubert, Volker Weisbach, Raymund E. Horch, Andreas Arkudas
For the purpose of skeletal muscle engineering, primary myoblasts (Mb) and adipogenic mesenchymal stem cells (ADSC) can be co-cultured and myogenically differentiated. Electrospun composite nanofiber scaffolds represent suitable matrices for tissue engineering of skeletal muscle, combining both biocompatibility and stability Although growth differentiation factor 11 (GDF11) has been proposed as a rejuvenating
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Computational analysis of missense variant CYP4F2*3 (V433M) in association with human CYP4F2 dysfunction: a functional and structural impact BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-05-09 Mahvash Farajzadeh-Dehkordi, Ladan Mafakher, Fatemeh Samiee-Rad, Babak Rahmani
Cytochrome P450 4F2 (CYP4F2) enzyme is a member of the CYP4 family responsible for the metabolism of fatty acids, therapeutic drugs, and signaling molecules such as arachidonic acid, tocopherols, and vitamin K. Several reports have demonstrated that the missense variant CYP4F2*3 (V433M) causes decreased activity of CYP4F2 and inter-individual variations in warfarin dose in different ethnic groups.
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Using RNA-seq to identify suitable housekeeping genes for hypoxia studies in human adipose-derived stem cells BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-04-17 Huan Ting Ong, Cecilia M. Prêle, Rodney J. Dilley
Hypoxic culture conditions have been used to study the impact of oxygen deprivation has on gene expression in a number of disease models. However, hypoxia response elements present in the promoter regions of some commonly used housekeeping genes, such as GAPDH and PGK1, can confound the relative gene expression analysis. Thus, there is ongoing debate as to which housekeeping gene is appropriate for
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SCAT8/miR-125b-5p axis triggers malignant progression of nasopharyngeal carcinoma through SCARB1 BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-04-03 Chunmao Jiang, Dandan Feng, Yu Zhang, Kun Yang, Xiaotong Hu, Qian Xie
Nasopharyngeal carcinoma is a tumor with high malignancy and poor prognosis, which severely affects the health of the patients. LncRNAs and microRNAs are crucial for the occurrence and development of nasopharyngeal carcinoma, which regulate the progression of nasopharyngeal carcinoma through the ceRNA network. SCARB1 plays an essential role in nasopharyngeal carcinoma. However, the mechanism underlying
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ARNTL2 upregulation of ACOT7 promotes NSCLC cell proliferation through inhibition of apoptosis and ferroptosis BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-03-31 Tao Wang, Kai Wang, Xu Zhu, Nan Chen
Recent studies have reported that the circadian transcription factor aryl hydrocarbon receptor nuclear translocator like 2 (ARNTL2) promotes the metastatic progression of lung adenocarcinoma. However, the molecular mechanisms of ARNTL2 in non-small cell lung cancer (NSCLC) cell growth and proliferation remain to be explored. The expression of ARNTL2 and acyl-CoA thioesterase 7 (ACOT7) in lung cancer
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Evolutionary relevance of single nucleotide variants within the forebrain exclusive human accelerated enhancer regions BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-03-29 Hizran Khatoon, Rabail Zehra Raza, Shoaib Saleem, Fatima Batool, Saba Arshad, Muhammad Abrar, Shahid Ali, Irfan Hussain, Neil H. Shubin, Amir Ali Abbasi
Human accelerated regions (HARs) are short conserved genomic sequences that have acquired significantly more nucleotide substitutions than expected in the human lineage after divergence from chimpanzees. The fast evolution of HARs may reflect their roles in the origin of human-specific traits. A recent study has reported positively-selected single nucleotide variants (SNVs) within brain-exclusive human
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The DNA demethylation-regulated SFRP2 dictates the progression of endometriosis via activation of the Wnt/β-catenin signaling pathway BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-03-29 Mei Yang, Lin Li, Xiaojie Huang, Hui Xing, Li Hong, Chunfan Jiang
Endometriosis cause decreases in life quality and pelvic pain in reproductive-age women. Methylation abnormalities played a functional role in the progression of endometriosis, this study aimed to explore the mechanisms mediated by abnormal methylation in the development of EMS. Next-generation sequencing dataset and methylation profiling dataset were used to screen out the key gene SFRP2. Western
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Pre-treatment with IL-6 potentiates β-cell death induced by pro-inflammatory cytokines BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-03-28 V. R. Oliveira, C. C. Paula, S. Taniguchi, F. Ortis
Type I Diabetes mellitus (T1D) is characterized by a specific destruction of β-cells by the immune system. During this process pro-inflammatory cytokines are released in the pancreatic islets and contribute for β-cells demise. Cytokine-induced iNOS activation, via NF-κB, is implicated in induction of β-cells death, which includes ER stress activation. Physical exercise has been used as an adjunct for
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Role of the human solute carrier family 14 member 1 gene in hypoxia-induced renal cell carcinoma occurrence and its enlightenment to cancer nursing BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-03-18 Jing Shi, Ruili Sha, Xilan Yang
Hypoxia is considered a critical contributor to renal cell carcinoma progression, including invasion and metastasis. However, the potential mechanisms by which it promotes invasion and metastasis have not yet been clarified. The purpose of this study was to investigate the role and mechanism of hypoxia-induced renal cell carcinoma and provide evidence-based medical proof for improvements to postoperative
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Cyclic tensile force modifies calvarial osteoblast function via the interplay between ERK1/2 and STAT3 BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-03-08 Xiaoyue Xiao, Shujuan Zou, Jianwei Chen
Mechanical therapies, such as distraction osteogenesis, are widely used in dental clinics. During this process, the mechanisms by which tensile force triggers bone formation remain of interest. Herein, we investigated the influence of cyclic tensile stress on osteoblasts and identified the involvement of ERK1/2 and STAT3. Rat clavarial osteoblasts were subjected to tensile loading (10% elongation,
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Urine-derived mesenchymal stem cells-derived exosomes enhances survival and proliferation of aging retinal ganglion cells BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-03-07 Qi-Qin Dan, Li Chen, Lan-Lan shi, Xiu Zhou, Ting-Hua Wang, Hua Liu
This study was designed to investigate to test the effect of exosomes from urine-derived mesenchymal stem cells (USCs) on the survival and viability of aging retinal ganglion cells (RGCs), and explored the preliminary related mechanisms. Primary USCs were cultured and identified by immunofluorescence staining. Aging RGCs models were established by D-galactose treatment and identified by β-Galactosidase
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RPL11 promotes non-small cell lung cancer cell proliferation by regulating endoplasmic reticulum stress and cell autophagy BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-03-03 Jie Chen, Changda Lei, Huahua Zhang, Xiaoyong Huang, Yang Yang, Junli Liu, Yuna Jia, Haiyan Shi, Yunqing Zhang, Jing Zhang, Juan Du
Abnormal biogenesis and ribosome free function of ribosomal proteins (RPs) is important for tumorgenesis and development. Ribosomal protein L11 (RPL11) is a component of ribosomal 60 S large subunit with different roles in different cancers. Here, we aimed to unravel the role of RPL11 in non-small cell lung cancer (NSCLC), especially those affecting cell proliferation. RPL11 expression in NCI-H1650
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Sperm capacitation and transcripts levels are altered by in vitro THC exposure BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-02-23 Vivien B. Truong, Ola S. Davis, Jade Gracey, Michael S. Neal, Jibran Y. Khokhar, Laura A. Favetta
Delta-9-tetrahydrocannabinol (THC) is the primary phytocannabinoid responsible for the psychoactive properties of cannabis and is known to interact with the endocannabinoid system, which is functionally present in the male reproductive system. Since cannabis consumption is the highest among reproductive aged males, the current study aimed to further investigate the effects of THC exposure to phenotypical
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The dual role of Nrf2 in melanoma: a systematic review BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-02-06 Zahra Malakoutikhah, Zahra Mohajeri, Nasim Dana, Shaghayegh Haghjooy Javanmard
Melanoma is the most lethal type of skin cancer that originates from the malignant transformation of melanocytes. Although novel treatments have improved patient survival in melanoma, the overall prognosis remains poor. To improve current therapies and patients outcome, it is necessary to identify the influential elements in the development and progression of melanoma. Due to UV exposure and melanin
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Hyperoxia exposure upregulates Dvl-1 and activates Wnt/β-catenin signaling pathway in newborn rat lung BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-02-02 Yuting Zhu, Yawen Li, Weilai Jin, Zhengying Li, Le Zhang, Yuanyuan Fang, Yanyu Zhang
Bronchopulmonary dysplasia is a serious and lifelong pulmonary disease in premature neonates that influences around one-quarter of premature newborns. The wingless-related integration site /β-catenin signaling pathway, which is abnormally activated in the lungs with pulmonary fibrosis, affects cell differentiation and lung development. Newborn rats were subjected to hyperoxia exposure. Histopathological
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Circ-ATL1 silencing reverses the activation effects of SIRT5 on smooth muscle cellular proliferation, migration and contractility in intracranial aneurysm by adsorbing miR-455 BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-01-30 Jichong Xu, Chun Fang
Alterations in vascular smooth muscle cells (VSMCs) contribute to the pathogenesis of intracranial aneurysms (IAs). However, molecular mechanisms underlying these changes remain unknown. The present study aimed to characterize the molecular mechanisms underlying VSMC-mediated IAs. Expression of the circular RNA circ-ATL1 and microRNA miR-455 was detected in IAs by RT-qPCR. Interactions between circ-ATL1
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HMGB1 mediates lipopolysaccharide-induced macrophage autophagy and pyroptosis BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-01-19 Jiawei Shang, Feng Zhao, Yongmei Cao, Feng Ping, Wei Wang, Yingchuan Li
Autophagy and pyroptosis of macrophages play important protective or detrimental roles in sepsis. However, the underlying mechanisms remain unclear. High mobility group box protein 1 (HMGB1) is associated with both pyroptosis and autophagy. lipopolysaccharide (LPS) is an important pathogenic factor involved in sepsis. Lentivirus-mediated HMGB1 shRNA was used to inhibit the expression of HMGB1. Macrophages
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N-Acetyl-L-cysteine facilitates tendon repair and promotes the tenogenic differentiation of tendon stem/progenitor cells by enhancing the integrin α5/β1/PI3K/AKT signaling BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2023-01-05 Lu, Kang, Zhou, Mei, Wang, Liyuan, Wang, Yang, Tang, Hong, He, Gang, Wang, Huan, Tang, Chuyue, He, Jie, Wang, Wei, Tang, Kanglai, Wang, Yunjiao, Deng, Zhongliang
Tendon injury is associated with oxidative stress, leading to reactive oxygen species (ROS) production and inflammation. N-acetyl-L-cysteine (NAC) is a potent antioxidant. However, how NAC affects the biological functions of tendon stem/progenitor cells (TSPCs) and tendon repair has not been clarified. The impacts of NAC on the viability, ROS production, and differentiation of TSPCs were determined
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The 3-O sulfation of heparan sulfate proteoglycans contributes to the cellular internalization of tau aggregates BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2022-12-24 Ferreira, Andreia, Royaux, Ines, Liu, Jian, Wang, Zhangjie, Su, Guowei, Moechars, Diederik, Callewaert, Nico, De Muynck, Louis
Considering the high correlation between the functional decline in Alzheimer’s disease (AD) and the propagation of aggregated tau protein, many research efforts are focused on determining the underlying molecular mechanisms of tau spreading. Heparan sulfate proteoglycans (HSPGs) were reported to mediate cellular uptake of tau aggregates. Specifically, the heparan sulfates (HS) sulfation plays a critical
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microRNA-338-3p suppresses lipopolysaccharide-induced inflammatory response in HK-2 cells BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2022-12-23 Wang, Jing, Li, Guokai, Lin, Min, Lin, Sheng, Wu, Ling
Inflammation is the most common cause of kidney damage, and inflammatory responses in a number of diseases are mediated by microRNA-338-3p (miR-338-3p). However, there are only a few reports which described the regulation of miR-338-3p in human proximal tubular cells. The goal of this study was to see how miR-338-3p affected lipopolysaccharide (LPS)-caused inflammatory response in HK-2 cells. LPS was
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Overexpression of lncRNA HOXA-AS2 promotes the progression of oral squamous cell carcinoma by mediating SNX5 expression BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2022-12-17 Li, Zhangyi
Oral squamous cell carcinoma (OSCC) is one of the most common head and neck cancers. Long non-coding RNA HOXA-AS2 (lncRNA HOXA-AS2) have been extensively studied in various cancers. However, the expression and function of HOXA-AS2 in OSCC still remain unknown. The aim of this study is to investigate the roles of HOXA-AS2 in OSCC. OSCC tissues and adjacent normal tissues were obtained from OSCC patients
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Overexpressed cold inducible RNA-binding protein improves cell viability and EGF expression in glial cells BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2022-12-16 Liu, Aijun, Li, Shouchun, Jiao, Yongcheng, Kong, Haibo, Zhang, Zhiwen
Cold inducible RNA-binding protein (CIRP) is a key protein in the hypothermic therapy. Highly expressed CIRP exerts a neuroprotective effect on neurons. The aim of this study is to provide the evidence of the protective effects of CIRP on the glial cells and explore the downstream pathway of CIRP. The results of this study demonstrated that the cell viability of the glial cells with CIRP overexpression
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CREB1 regulates KPNA2 by inhibiting mir-495-3p transcription to control melanoma progression BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2022-12-15 Geng, Xuerui, Qiu, Xiujuan, Gao, Jun, Gong, Zhifan, Zhou, Xiaogang, Liu, Chunlei, Luo, Haichao
Melanoma is a common type of skin cancer, and its incidence is increasing gradually. Exploring melanoma pathogenesis helps to find new treatments. We aimed to explore the potential molecular mechanisms by which CREB1 regulates melanoma. TransmiR and ALGGEN were used to predict targets of CREB1 in the promoter of miR-495-3p or miR-495-3p and KPNA2, and a dual-luciferase reporter assay was performed
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Metabolic analysis of the regulatory mechanism of sugars on secondary flowering in Magnolia BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2022-12-14 Xuan, Lingjuan, Wang, Qianqian, Liu, Zhigao, Xu, Bin, Cheng, Shaoyu, Zhang, Yingjia, Lu, Danying, Dong, Bin, Zhang, Dongmei, Zhang, Lang, Ma, Jingjing, Shen, Yamei
Magnolia, a traditional and important ornamental plant in urban greening, has been cultivated for about 2000 years in China for its elegant flower shape and gorgeous flower color. Most varieties of Magnolia bloom once a year in spring, whereas a few others, such as Magnolia liliiflora Desr. ‘Hongyuanbao’, also bloom for the second time in summer or early autumn. Such a twice flowering trait is desirable
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Exogenous 8-hydroxydeoxyguanosine attenuates doxorubicin-induced cardiotoxicity by decreasing pyroptosis in H9c2 cardiomyocytes BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2022-12-14 Hwang, Soyoung, Kim, Se-Hee, Yoo, Kwai Han, Chung, Myung-Hee, Lee, Jin Woo, Son, Kuk Hui
Doxorubicin (DOX), which is widely used in cancer treatment, can induce cardiomyopathy. One of the main mechanisms whereby DOX induces cardiotoxicity involves pyroptosis through the NLR family pyrin domain containing 3 (NLRP3) inflammasome and gasdermin D (GSDMD). Increased NAPDH oxidase (NOX) and oxidative stress trigger pyroptosis. Exogenous 8-hydroxydeoxyguanosine (8-OHdG) decreases reactive oxygen
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Ceramide induces pyroptosis through TXNIP/NLRP3/GSDMD pathway in HUVECs BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2022-12-14 Liu, Fangfang, Zhang, Yangyang, Shi, Yining, Xiong, Kai, Wang, Fugui, Yang, Jin
Pyroptosis of endothelial cells is a new cause of endothelial dysfunction in multiple diseases. Ceramide acts as a potential bioactive mediator of inflammation and increases vascular endothelial permeability in many diseases, whether it can aggravate vascular endothelial injury by inducing cell pyroptosis remains unknown. This study was established to explore the effects of C8-ceramide (C8-Cer) on
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Comparison of adhesion of thawed and cultured synovial mesenchymal stem cells to the porcine meniscus and the relevance of cell surface microspikes BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2022-12-12 Fujii, Shunichi, Endo, Kentaro, Ozeki, Nobutake, Sakamaki, Yuriko, Kohno, Yuji, Mizuno, Mitsuru, Katano, Hisako, Tsuji, Kunikazu, Koga, Hideyuki, Sekiya, Ichiro
Placement of a cultured synovial mesenchymal stem cell (MSC) suspension on a repaired meniscus for 10 min accelerated meniscus repair. Upon placement of the MSC suspension on the meniscus, microspikes projecting from the MSC surface trap meniscus fibers and promote MSC adhesion. Thawed cryopreserved MSCs are preferred materials for meniscus repair, as they can be transplanted without additional culture
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RUNX3 mediates keloid fibroblast proliferation through deacetylation of EZH2 by SIRT1 BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2022-12-07 Liu, Hanye, Yan, Guanghai, Li, Li, Wang, Dandan, Wang, Yu, Jin, Shan, Jin, Zhehu, Li, Liangchang, Zhu, Lianhua
Keloid is a benign proliferative fibrous disease featured by excessive fibroblast proliferation after skin injury. However, the mechanism of abnormal cell proliferation is still unclear. Herein, we investigated the mechanism of abnormal proliferation in keloids involving Sirtuin 1(SIRT1)/ Zeste Homolog 2 (EZH2)/ Runt-related transcription factor 3 (RUNX3). HE staining was used to observe the histopathological
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Lncap-AI prostate cancer cell line establishment by Flutamide and androgen-free environment to promote cell adherent BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2022-11-28 Wang, Huifeng, Wei, Xihua, Zhang, Die, Li, Weidong, Hu, Yanling
To establish castration-resistant prostate cancer (CRPC) - Lncap androgen-independent (AI) cell line from Lncap androgen-dependent (AD) cell line, and explore the different molecular biological between these two cell lines. The Lncap-AD cell line was cultured and passaged 60 times over 16 months. The morphology of the Lncap-AI cell line was observed. AR levels identification were detected in qRT-PCR
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CARD9 contributes to ovarian cancer cell proliferation, cycle arrest, and cisplatin sensitivity BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2022-11-28 Wang, Yanming, Wang, Chao, Zhu, Yan
Ovarian cancer recurrence and chemotherapy resistance are still urgent issues, and exploring the mechanisms of metastasis and chemotherapy resistance is beneficial to the development of therapeutic methods. Caspase recruitment domain family member 9 (CARD9) and homeobox B5 (HOXB5) are related and both are upregulated in ovarian cancer. This study aimed to define their functions in ovarian cancer cell
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Ribosomal protein RPL5 regulates colon cancer cell proliferation and migration through MAPK/ERK signaling pathway BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2022-11-16 Zhang, Huahua, Liu, Junli, Dang, Qingqing, Wang, Xueru, Chen, Jie, Lin, Xiaoyin, Yang, Na, Du, Juan, Shi, Haiyan, Liu, Yong, Han, Jiming
Abnormal expression of ribosomal proteins has an important regulatory effect on the progression of cancer. RPL5 is involved in the progression of various malignancies, however, the role of RPL5 in colon cancer remains is still unclear. Data from TCGA and GTEx databases were used to analyze the RPL5 expression in pan-cancer. The expression level of RPL5 in clinical colon cancer tissue samples and human
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High levels of follicular fluid testosterone could impair oocyte developmental competency via affecting aryl hydrocarbon receptor pathway in PCOS patients BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2022-11-11 Eini, Fatemeh, kutenaei, Maryam Azizi, Foroutan, Tahereh, Salehi, Ensieh
Although hormonal and metabolic dysfunction have been recognized as a possible cause of polycystic ovarian syndrome (PCOS), the associations between hyperandrogenism and aryl hydrocarbon receptor (Ahr) signaling pathway remains controversial. The current study aimed to investigate the effect of hyperandrogenism on oocyte developmental competency via regarding Ahr signaling downstream pathway in granulosa
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Downregulation of miR-451 in cholangiocarcinoma help the diagnsosi and promotes tumor progression BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2022-11-09 Guo, Dengfang, Wang, Qingling, Huang, Jiancheng, Hu, Zhanglin, Chen, Chun, Zhang, Chun, Lin, Feng
Cholangiocarcinoma is a kind of invasive malignant tumor followed by hepatocellular carcinoma. miR-451 was suggested to function as regulator in various human tumors, but its role in mediating tumor progression and predicting the prognosis of cholangiocarcinoma remains unknown. The clinical significance and biological function of miR-451 in cholangiocarcinoma were assessed in this study. The tissue
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Acetaminophen changes the RNA m6A levels and m6A-related proteins expression in IL-1β-treated chondrocyte cells BMC Mol. Cell Biol. (IF 2.8) Pub Date : 2022-10-27 Gao, Jie, Li, Yan, Liu, Zijin, Wang, Dong, Zhang, Huawu
Acetaminophen is commonly recommended for the early analgesia of osteoarthritis. However, the molecular mechanism by which it acts remains unknown. The aim of this study is to investigate the effect of acetaminophen on inflammation and extracellular matrix degradation in human chondrocytes, and the possible molecular mechanisms involved in its effect. The normal chondrocyte cell line C28/I2 was treated