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Accuracy of Ex-vivo Fluorescence Confocal Microscopy in Margin Assessment of Solid Tumors: A Systematic Review. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-11-15 Matthew Au,Ricardo Almeida-Magana,Tarek Al-Hammouri,Aiman Haider,Greg Shaw
Fluorescence confocal microscopy (FCM) is a novel technology that enables rapid high-resolution digital imaging of non-formalin-fixed tissue specimens and offers real-time positive surgical margin identification. In this systematic review, we evaluated the accuracy metrics of ex vivo FCM for intraoperative margin assessment of different tumor types. A systematic search of MEDLINE via PubMed, Embase
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Additional Insights Into the Role of Osteocalcin in Osteoblast Differentiation and in the Early Steps of Developing Alveolar Process of Rat Molars. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-11-12 Gisela Rodrigues da Silva Sasso,Rinaldo Florencio-Silva,José Paulo de Pizzol-Júnior,Cristiane Damas Gil,Manuel de Jesus Simões,Estela Sasso-Cerri,Paulo Sérgio Cerri
This study investigated whether osteocalcin (OCN) is present in osteoblast precursors and its relationship with initial phases of alveolar process formation. Samples of maxillae of 16-, 18-, and 20-day-old rat embryos (E16, E18, and E20, respectively), and 05-, 10-, and 15-day-old postnatal rats (P05, P10, and P15, respectively) were fixed and embedded in paraffin or araldite. Immunohistochemistry
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Lineage Tracing Identifies Dynamic Contribution of Endothelial Cells to Cardiac Valve Mesenchyme During Development. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-11-01 Xiaojie Yang,Furong Lu
Heart valve disease is an important cause of morbidity and mortality among cardiac patients worldwide. However, the pathogenesis of heart valve disease is not clear, and a growing body of evidence hints at the importance of the genetic basis and developmental origins of heart valve disease. Therefore, understanding the developmental mechanisms that underlie the formation of heart valves has important
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Hyperspectral Raman Imaging for Automated Recognition of Human Renal Amyloid. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-10-13 Jeong Hee Kim,Chi Zhang,C John Sperati,Ishan Barman,Serena M Bagnasco
In the clinical setting, routine identification of the main types of tissue amyloid deposits, light-chain amyloid (AL) and serum amyloid A (AA), is based on histochemical staining; rarer types of amyloid require mass spectrometry analysis. Raman spectroscopic imaging is an analytical tool, which can be used to chemically map, and thus characterize, the molecular composition of fluid and solid tissue
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Immunohistochemical Study of Airways Fibrous Remodeling in Smoking Mice. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-10-11 Emilia Balzano,Giovanna De Cunto,Chiara Goracci,Barbara Bartalesi,Eleonora Cavarra,Giuseppe Lungarella,Monica Lucattelli
The fibrotic remodeling in chronic obstructive pulmonary disease (COPD) is held responsible for narrowing of small airways and thus for disease progression. Oxidant damage and cell senescence factors are recently involved in airways fibrotic remodeling. Unfortunately, we have no indications on their sequential expression at anatomical sites in which fibrotic remodeling develops in smoking subjects
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Enteric Nervous System: Identification of a Novel Neuronal Sensory Network in the Duodenal Epithelium. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-10-04 Víctor Salazar,Pura Bolaños,Jesús R Del Castillo
The communication between the intestinal epithelium and the enteric nervous system has been considered indirect. Mechanical or chemical stimuli activate enteroendocrine cells inducing hormone secretion, which act on sub-epithelial nerve ends, activating the enteric nervous system. However, we identified an epithelial cell that expresses NKAIN4, a neuronal protein associated with the β-subunit of Na+/K+-ATPase
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Spatial and Temporal Expression of Ectodysplasin-A Signaling Pathway Members During Mandibular Condylar Development in Postnatal Mice. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-09-20 Ludan Xing,Yuan Liu,Jiayan Wu,Chenyu Song,Beizhan Jiang
A growing body of evidence emerging supported that ectodysplasin-A (EDA) signaling pathway contributed to craniofacial development. However, their expression in condyle has not been elucidated yet. This study investigated the expression patterns of EDA, EDA receptor (EDAR), and EDAR-associated death domain (EDARADD) in condyle of postnatal mice. Histological staining and micro-computed tomography (CT)
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Tracking of Prosaposin, a Saposin Precursor, in Rat Testis. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-09-20 Kimiko Yamamiya,Xuan Li,Hiroaki Nabeka,Sakirul Khan,Farzana Khan,Hiroyuki Wakisaka,Shoichiro Saito,Fumihiko Hamada,Seiji Matsuda
We tracked prosaposin (PSAP), a trophic factor, using an antibody specific to its proteolytic portion and an antibody to sortilin that traffics PSAP only to the lysosome. Immunostaining revealed that PSAP was distributed mainly on the basal side of seminiferous tubules, where many Sertoli cells and pachytene spermatocytes contained PSAP and its distribution differed depending on the stage of the spermatogenic
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Trop-2 Is Highly Expressed in Early Luminal-Like Breast Cancer. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-09-07 Rafael C Coelho,Rubia D Ruppenthal,Marcia S Graudenz
Trop-2, a transmembrane glycoprotein, has been identified in human epithelial cells as a contributor to tumor growth and unfavorable prognosis in breast cancer (BC). Our study aimed to assess the expression of Trop-2 protein via immunohistochemistry (IHC) and correlate it with clinicopathological features in early luminal-like BC. We conducted a cross-sectional study evaluating Trop-2 protein expression
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Paradoxical Expression of R-10G-reactive Antigen in Human Testicular Embryonal Carcinoma. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-09-07 Akifumi Muramoto,So Inamura,Hitomi Hoshino,Naoki Terada,Motohiro Kobayashi
Thus far, several monoclonal antibodies directed against cell-surface carbohydrate antigens have been generated. Among them, R-10G reportedly reacts selectively with human embryonic stem and induced pluripotent stem cells, but not with embryonal carcinoma (EC) cells. However, EC cells derived from patients' EC tumors may exhibit varying levels of R-10G-reactive antigen expression. Thus, we asked whether
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Gelatin Zymography Can Be Performed on Fixed Brain Tissue. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-08-20 Zhenzhou Chen,Dennis Chuang,Shanyan Chen,Qiwei He,Brittany N Tomlison,Jiankun Cui,Zezong Gu
Gelatin zymography is widely used to detect gelatinase activity, which is performed on unfixed tissue because it is assumed that fixation inactivates enzymes. However, using fixed tissues has several advantages over using fresh tissues for such prevention of tissue decay, thereby preserving the proteins as well as the morphology and structure of the specimens. In this study, we investigated the effects
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Two Transient Receptor Potential Channels at Focal Adhesions. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-08-18 Ioli Mitsou,Cathrine Rein Carlson,Hinke A B Multhaupt,Cord Brakebusch,John R Couchman
Recently there have been reports that identify two transient receptor potential channels in cell-matrix junctions known as focal adhesions. These are the calcium channel TRP canonical 7 and the calcium-activated monovalent ion channel, TRP melastatin (TRPM) 4. Here, we report on the occurrence of TRPM4 in focal adhesions of fibroblasts. Of three commercial antibodies recognizing this channel, only
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Corrigendum to JHC 71:7 July 2023 Front Cover's Tagline. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-08-08
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Reflections on the Applications of Immunohistochemistry to Understanding the Maintenance of Electrochemical Gradients in the Inner Ear. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-08-03 Bradley A Schulte
The author of the accompanying classic paper from the Journal of Histochemistry and Cytochemistry (Crouch JJ, Sakaguchi N, Lytle C, Schulte BA. Immunohistochemical localization of the Na-K-Cl Co-transporter (NKCC1) in the Gerbil Inner Ear. Journal of Histochemistry & Cytochemistry 1997;45(6):773-778) comments on how the immunohistochemical techniques used in the study provided critical new information
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5hmC Immunohistochemistry: A Predictor of TERT Promoter Mutational Status in Follicular Thyroid Carcinoma? J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-07-24 Martin Hysek,Samuel L Hellgren,Vincenzo Condello,Yiyi Xu,Catharina Larsson,Jan Zedenius,C Christofer Juhlin
Telomerase reverse transcriptase (TERT) gene aberrancies correlate to adverse prognosis in follicular thyroid carcinoma (FTC). As loss of 5-hydroxymethylcytosine (5hmC) has been associated with TERT promoter mutations in papillary thyroid carcinoma, this study sought to analyze the levels of 5hmC in a cohort of follicular thyroid tumors with available TERT data. A total of 29 tumors (26 FTCs, 2 follicular
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Altered Expression of Heme Oxygenase 2 in Heme Oxygenase 1-deficient Mouse Embryos. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-07-22 Meenakshi Rana,Divya Bajaj,Pooja Choubey,Sidhant Jain,Sharmila Basu-Modak
Heme oxygenases (Hmoxs) are enzymes that catalyze the first and rate-limiting step in the degradation of heme to carbon monoxide, iron, and biliverdin. The two main isozymes, namely Hmox1 and Hmox2, are encoded by two different genes. Mutation of the Hmox1 gene in mice is known to cause extensive prenatal lethality, and limited information is available about the expression of Hmox proteins in developing
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SSEA-1 Correlates With the Invasive Phenotype in Breast Cancer. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-07-21 Katharina T Kohler,Anna A Møller Hansen,Jiyoung Kim,René Villadsen
The glycan moiety Lewis X (LeX) has been implicated in defining progenitor cells as well as playing a role in the progression of solid tumors, including breast cancer. Here, we used the original stage-specific embryonic antigen-1 (SSEA-1) antibody, MC-480, targeting the LeX motif to examine the expression pattern of this marker within the context of a differentiation hierarchy as well as functional
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Commentary on a Classic JHC Article on Intracellular Hyaluronan Associated With the Mitotic Spindle. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-07-16 Stephen P Evanko,Thomas N Wight
The authors of the accompanying classic paper from the Journal of Histochemistry and Cytochemistry (Evanko SP, Wight TN. Intracellular Localization of Hyaluronan in Proliferating Cells. Journal of Histochemistry & Cytochemistry. 1999;47[10]:1331-1341) comment on the impact and significance of their findings on the intracellular localization of hyaluronan in arterial smooth muscle cells using immunohistochemical
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Absence of E-Cadherin and β-Catenin in the Basal Plasma Membrane of Collecting Duct Cells During NDI Development and Recovery. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-07-13 Xabier Sørtvedt,Rikke Nielsen,Jeppe Praetorius,Birgitte M Christensen
Lithium (Li) induces severe polyuria and polydipsia in up to 40% of patients undergoing Li treatment. In rats, Li treatment induces a reversible cellular remodeling of the collecting duct (CD), decreasing the fraction of principal-to-intercalated cells. To investigate the potential role of adherens junction proteins, we performed immunohistochemistry on kidney cross-sections from rats treated with
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Expression of Protein Markers in Spermatogenic and Supporting Sertoli Cells Affected by High Abdominal Temperature in Cryptorchidism Model Mice. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-07-10 Arunothai Wanta,Kazuhiro Noguchi,Taichi Sugawara,Kayoko Sonoda,Suthat Duangchit,Tomohiko Wakayama
Cryptorchidism is a congenital abnormality resulting in increased rates of infertility and testicular cancer. We used cryptorchidism model mice that presented with the translocation of the left testis from the scrotum to the abdominal cavity. Mice underwent the surgical procedure of the left testis at day 0 and were sacrificed at days 3, 5, 7, 14, 21, and 28 post-operatively. The weight of the left
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The Battle Against Quenching and Fading for Fluorescence: A Model of How to Evaluate the Fluorophore's Behavior. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-07-07 Gloria E Hoffman
The fading and quenching of fluorescence intensity has been a major problem in the use of fluorescein isothiocyanate (FITC) for immunofluorescence cytochemical techniques, especially with laser confocal microscopy. The companion article by Longin et al. provided an empirical approach to overcoming this problem. The present commentary highlights the significance of the Longin et al. article when it
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SS18-SSX Antibody: A Useful Tool to Save Time and Reduce Costs in Synovial Sarcoma Diagnosis. Proposal of a Novel Diagnostic Algorithm. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-06-26 Giulia Orlando,Federica Santoro,Alessandra Linari,Cristian Tampieri,Ludovica Verdun di Cantogno,Simone De Meo,Nicola Ratto,Giovanni Grignani,Mauro Papotti,Rebecca Senetta
Synovial sarcoma is a rare malignant mesenchymal neoplasm mostly affecting young adults, characterized by a specific translocation which results in the fusion of the SS18 gene on chromosome 18 with one of the three highly homologous SSX genes on chromosome X. Its morphological diagnosis, especially in monophasic or poorly differentiated variants, can be challenging because histological features often
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Developmental Expression of the Cell Cycle Regulator p16INK4a in Retinal Glial Cells: A Novel Marker for Immature Ocular Astrocytes? J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-06-23 Cristina Martinez-Fernandez de la Camara,Tina Storm,Ahmed Salman,Thomas Burgoyne,Martin Qvist Rasmussen,Harry O Orlans,Angela J Russell,Stephen G Davies,Alun R Barnard,Robert E MacLaren
Retinal astrocytes are vital for neuronal homeostasis in the retina. Together with Müller glia, they provide retinal cells with neurotrophic factors, antioxidative support, and defense mechanisms such as the formation of the blood-retinal barrier. Substantial heterogeneity of astrocyte morphology and function represents a challenge for identification of distinct subtypes which may be potential targets
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Colonic Myenteric Plexus Neurodegeneration and Minor Colon Inflammation in Trimethyltin-induced Rat Model of Neurodegeneration. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-06-15 Dian Eurike Septyaningtrias,Hilizza Awalina Zulfa,Mahayu Firsty Ramadhani,Sumaryati,Dewi Sulistyawati,Dewi Kartikawati Paramita,Yustina Andwi Ari Sumiwi,Rina Susilowati
Gastrointestinal symptoms are common health problems found during aging and neurodegenerative diseases. Trimethyltin-induced rat is known as an animal model of hippocampal degeneration with no data on enteric neurodegeneration. This study aimed to investigate the effect of trimethyltin (TMT) induction on the gastrointestinal tract. A 28-day animal study with male Sprague-Dawley rats (3 months old,
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Commentary on a Classic JHC Article on the Histochemical Measurement of DNA Content in Cells. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-06-13 Cornelis J F van Noorden
This article comments on the significance of a highly cited review article on DNA cytochemical quantitation that was published in the Journal of Histochemistry and Cytochemistry in 2002 (David C. Hardie, T. Ryan Gregory, and Paul D.N. Hebert. From pixels to picograms: A beginners' guide to genome quantification by Feulgen image analysis densitometry.
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Expression of JCAD and EGFR in Perineurial Cell-Cell Junctions of Human Inferior Alveolar Nerve. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-06-13 Yujiro Hiraoka,Megumi Matsumura,Yasumasa Kakei,Daisuke Takeda,Manabu Shigeoka,Akira Kimoto,Takumi Hasegawa,Masaya Akashi
Although perineurium has an important role in maintenance of the blood-nerve barrier, understanding of perineurial cell-cell junctions is insufficient. The aim of this study was to analyze the expression of junctional cadherin 5 associated (JCAD) and epidermal growth factor receptor (EGFR) in the perineurium of the human inferior alveolar nerve (IAN) and investigate their roles in perineurial cell-cell
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Commentary on a Classic JHC Article on the Development of Highly Sensitive Fluorochrome-labeled Tyramides for Immunocytochemistry. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-05-18 Kevin A Roth
This commentary reflects on the significance and impact of the highly cited companion article that was published in the Journal of Histochemistry and Cytochemistry in 1997 (Gijlswijk RPM et al. Fluorochrome-labeled Tyramides: Use in Immunocytochemistry and Fluorescence In Situ Hybridization. Journal of Histochemistry & Cytochemistry. 1997;45(3):375-382).
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Prematurity and Hyperoxia Have Different Effects on Alveolar and Microvascular Lung Development in the Rabbit. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-05-18 Giacomo Rößler,Jonas Labode,Yannick Regin,Thomas Salaets,André Gie,Jaan Toelen,Christian Mühlfeld
Bronchopulmonary dysplasia (BPD) is a developmental disorder of infants born prematurely, characterized by disrupted alveolarization and microvascular maturation. However, the sequence of alveolar and vascular alterations is currently not fully understood. Therefore, we used a rabbit model to evaluate alveolar and vascular development under preterm birth and hyperoxia, respectively. Pups were born
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Epitope Lability of Phosphorylated Biomarkers of the DNA Damage Response Pathway Results in Increased Vulnerability to Effects of Delayed or Incomplete Formalin Fixation J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-04-29 Elizabeth J. Wiseman, Jennifer I. Moss, James Atkinson, Hana Baakza, Emily Hayes, Sophie E. Willis, Paul M. Waring, Jaime Rodriguez Canales, Gemma N. Jones
Phosphorylated biomarkers are crucial for our understanding of drug mechanism of action and dose selection during clinical trials, particularly for drugs that target protein kinases, such as DNA-da...
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Improving Yields in Multi-analyte Extractions by Utilizing Post-homogenized Tissue Debris J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-04-29 Ala Petersons, Joseph Carlson, William Mathieson
In multi-analyte extractions, tissue is typically homogenized in a lysis buffer, and then DNA, RNA, and protein are purified from the supernatant. However, yields are typically lower than in dedica...
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The Distribution of Foxp3 and CD68 in Preeclamptic and Healthy Placentas: A Histomorphological Evaluation J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-04-18 Yasemin Ersoy Canillioglu, Gozde Erkanli Senturk, Hakan Sahin, Sadik Sahin, Yasemin Seval-Celik
Preeclampsia is a complication of pregnancy that affects 3–5% of pregnancies and is one of the major causes of maternal/neonatal mortality and morbidities worldwide. We aimed to investigate the dis...
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Spatial Distribution of SARS-CoV-2 Receptors and Proteases in Testicular Cells J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-04-07 Maria Rita Ribeiro, Ana Margarida Calado, Ângela Alves, Rute Pereira, Mário Sousa, Rosália Sá
Coronavirus disease (COVID-19) is caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). SARS-CoV-2 RNA has been found in the human testis on occasion, but subgenomic SARS-CoV-2 an...
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Betaine Treatment Prevents TNF-α-Mediated Muscle Atrophy by Restoring Total Protein Synthesis Rate and Morphology in Cultured Myotubes J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-04-03 Andrea Di Credico, Giulia Gaggi, Pascal Izzicupo, Daniela Vitucci, Pasqualina Buono, Angela Di Baldassarre, Barbara Ghinassi
Skeletal muscle atrophy is represented by a dramatic decrease in muscle mass, and it is related to a lower life expectancy. Among the different causes, chronic inflammation and cancer promote prote...
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Inhibition of RANKL Expression in Osteocyte-like Differentiated Tumor Cells in Giant Cell Tumor of Bone after Denosumab Treatment J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-03-27 Takashi Noguchi, Akio Sakamoto, Yoshiki Murotani, Koichi Murata, Masahiro Hirata, Yosuke Yamada, Junya Toguchida, Shuichi Matsuda
Giant cell tumors of bone (GCTBs) are locally aggressive tumors with the histological features of giant cells and stromal cells. Denosumab is a human monoclonal antibody that binds to the cytokine ...
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Expression of β-Catenin, E-Cadherin, and α-Smooth Muscle Actin in Basal Cell Carcinoma Before Photodynamic Therapy in Non-recurrent and Recurrent Tumors: Exploring the Ability of Predicting Photodynamic Therapy outcome J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-03-24 Erik Mørk, Patricia Mjønes, Olav A. Foss, Ingeborg M. Bachmann, Eidi Christensen
Photodynamic therapy (PDT) is an effective and cosmetically beneficial treatment of low-risk basal cell carcinomas (BCCs). To optimize PDT response, it is important to correctly select tumors. We s...
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Multiplex Immunofluorescence Image Quality Checking Using DAPI Channel–referenced Evaluation J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-03-24 Jun Jiang, Raymond Moore, Clarissa E. Jordan, Ruifeng Guo, Rachel L. Maus, Hongfang Liu, Ellen Goode, Svetomir N. Markovic, Chen Wang
Multiplex immunofluorescence (MxIF) images provide detailed information of cell composition and spatial context for biomedical research. However, compromised data quality could lead to research bia...
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The Application of Guanidinium to Improve Biomolecule Quality in Fixed, Paraffin-embedded Tissue J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-03-04 Joon-Yong Chung, Kyungeun Kim, Kris Ylaya, Katharine E. Walker-Bawa, Candice Perry, Robert A. Star, Stephen M. Hewitt
Neutral buffered formalin (NBF) is the most common fixative in clinical applications. However, NBF damages proteins and nucleic acids, limiting the quality of proteomic and nucleic acid-based assay...
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Thioflavin S Staining and Amyloid Formation Are Unique to Mixed Tauopathies J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-03-02 Kimberly L. Fiock, Ryan K. Betters, Marco M. Hefti
Tau phosphorylation, aggregation, and toxicity are the main drivers of neurodegeneration in multiple tauopathies, including Alzheimer’s disease (AD) and frontotemporal lobar degeneration with tau. ...
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NKG2D Ligand Expression Induced by Oxidative Stress Mitigates Cutaneous Ischemia–Reperfusion Injury J. Histochem. Cytochem. (IF 3.2) Pub Date : 2023-02-10 Keishi Makita, Noriyuki Otsuka, Utano Tomaru, Koji Taniguchi, Masanori Kasahara
Pressure ulcers represent a crucial clinical problem, especially in hospitalized patients. Ischemia–reperfusion (I-R) is an important cause of these lesions. Natural killer (NK), invariant NK T (iN...
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Commentary on "Antigen Retrieval Immunohistochemistry: Past, Present, and Future". J. Histochem. Cytochem. (IF 3.2) Pub Date : 2022-12-29 Clive R Taylor,Shan-Rong Shi
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Long Hanging Structure of Collagen VII Connects the Elastic Fibers and the Basement Membrane in Young Skin Tissue J. Histochem. Cytochem. (IF 3.2) Pub Date : 2022-12-21 Takeshi Tohgasaki, Shino Nishizawa, Shinya Kondo, Shioji Ishiwatari, Tetsuhito Sakurai
Aging leads to substantial structural changes in the skin. Elastic fibers maintain skin structure, but their degeneration and loss of function with age result in wrinkle formation and loss of skin ...
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Commentary on "Application of Photoshop-based Image Analysis to Quantification of Hormone Receptor Expression in Breast Cancer". J. Histochem. Cytochem. (IF 3.2) Pub Date : 2022-12-22 Allen M Gown
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The Graham and Karnovsky Horseradish Peroxidase Ultrastructural Method: A Premier JHC Citation Classic J. Histochem. Cytochem. (IF 3.2) Pub Date : 2022-12-21 Dale R. Abrahamson
This commentary briefly reviews the background for the development of the horseradish peroxidase–diaminobenzidine tetrahydrochloride histochemical method originally described by Graham and Karnovsk...
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Commentary on "Classifications of Anterior Pituitary Cell Types With Immunoenzyme Histochemistry": Dr. Paul Nakane Blazed the Trail to Modern Technology. J. Histochem. Cytochem. (IF 3.2) Pub Date : 2022-12-21 Gwen V Childs
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A Forgotten Principle in Immunocytochemistry: Optimal Dilution J. Histochem. Cytochem. (IF 3.2) Pub Date : 2022-12-13 Bertalan Dudás, Malcolm Lane, Nikitha Mupparaju, Hye Min Kim, Istvan Merchenthaler
Immunocytochemical (ICC) techniques are frequently used in basic and clinical research. Here, we focus on the importance of using antisera/antibodies at optimal dilutions to achieve specificity and...
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Phosphotungstic Acid–treated Picrosirius Red Staining Improves Whole-slide Quantitative Analysis of Collagen in Histological Specimens J. Histochem. Cytochem. (IF 3.2) Pub Date : 2022-11-26 Yui Mukade, Sayaka Kobayashi, Yoshimi Nishijima, Kiminori Kimura, Akira Watanabe, Hayato Ikota, Ken Shirabe, Hideaki Yokoo, Masanao Saio
We tried to prevent nonspecific nuclear staining (NS-NS) of picrosirius red (PSR) staining by treating the specimens with one of the heteropoly acids phosphotungstic acid (PTA). We analyzed a total...
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Abnormal Expression of an Insulin Synthesizing Enzyme in Islets of Adult Autoantibody Positive Donors J. Histochem. Cytochem. (IF 3.2) Pub Date : 2022-11-05 Gladys Teitelman
The observation that the two active forms of proprotein convertase 1/3 (PC1/3) were differentially expressed in beta cells of normal islets raised the possibility that this heterogeneity is lost du...
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Aberrant Expression of Thymosin Beta-4 Correlates With Advanced Disease and BRAF V600E Mutation in Thyroid Cancer J. Histochem. Cytochem. (IF 3.2) Pub Date : 2022-11-02 Chi-Yu Kuo, Jie-Yang Jhuang, Wen-Chien Huang, Shih-Ping Cheng
Thymosin beta-4 (TMSB4X) was recently identified as a differentially expressed gene between malignant and non-malignant thyroid cells via single-cell RNA sequencing. In the present study, we aimed ...
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Expression and Clinical Significance of Spi-B in B-cell Acute Lymphoblastic Leukemia J. Histochem. Cytochem. (IF 3.2) Pub Date : 2022-09-28 Yuzuru Ariga, Shulin Low, Hitomi Hoshino, Tsutomu Nakada, Tomoya O. Akama, Akifumi Muramoto, Mana Fukushima, Takahiro Yamauchi, Yusei Ohshima, Motohiro Kobayashi
Spi-B, a member of the E26 transformation-specific (ETS) family of transcription factors, plays an important role in B cell differentiation. Spi-B also functions in development of diffuse large B-c...
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Human Pulmonary Vein Myocardial Sleeve Autonomic Neural Density and Cardiovascular Mortality J. Histochem. Cytochem. (IF 3.2) Pub Date : 2022-09-24 Denis Depes, Ari Mennander, Rauha Vehniäinen, Timo Paavonen, Ivana Kholová
Myocardial sleeves around pulmonary veins (PVs) are highly innervated structures with heterogeneous morphological and electrophysiological characteristics. Autonomic nerve dysfunction in the myocar...
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Effect of Antigen Retrieval on Genomic DNA From Immunodissected Samples J. Histochem. Cytochem. (IF 3.2) Pub Date : 2022-09-21 Donald J. Johann, Ik Jae Shin, Adam Roberge, Sarah Laun, Erich A. Peterson, Meei Liu, Matthew A. Steliga, Jason Muesse, Michael R. Emmert-Buck, Michael A. Tangrea
Immunohistochemical (IHC) staining is an established technique for visualizing proteins in tissue sections for research studies and clinical applications. IHC is increasingly used as a targeting st...
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Tuft Cells Are Present in Submandibular Glands Across Species J. Histochem. Cytochem. (IF 3.2) Pub Date : 2022-08-21 Harim Tavares dos Santos, Kihoon Nam, Frank M. Maslow, Travis Small, Tabitha L.I. Galloway, Laura M. Dooley, Patrick T. Tassone, Robert P. Zitsch, III, Gary A. Weisman, Olga J. Baker
Tuft cells are bottle-shaped, microvilli-projecting chemosensory cells located in the lining of a variety of epithelial tissues and, following their identification approximately 60 years ago, have ...
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Expression of Calbindin-D28K in the Developing and Adult Mouse Cochlea J. Histochem. Cytochem. (IF 3.2) Pub Date : 2022-08-16 Wenjing Liu, Huijun Chen, Xin Zhu, Hao Yu
Herein, we aimed to use double-labeling immunofluorescence to describe the expression pattern of Calbindin-D28K (CaBP28K) in the mouse cochlea from late embryonic (E) stages to the adulthood. CaBP2...
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Rapid Histological Assessment of Prostate Specimens in the Three-dimensional Space by Hydrophilic Tissue Clearing and Confocal Microscopy J. Histochem. Cytochem. (IF 3.2) Pub Date : 2022-07-30 Yu-Ching Peng, Yu-Chieh Lin, Yu-Ling Hung, Chien-Chung Fu, Margaret Dah-Tsyr Chang, Yen-Yin Lin, Teh-Ying Chou
Microscopic examination of biopsied and resected prostatic specimens is the mainstay in the diagnosis of prostate cancer. However, conventional analysis of hematoxylin and eosin (H&E)-stained tissu...
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Quantitative Assessment of the Apical and Basolateral Membrane Expression of VEGFR2 and NRP2 in VEGF-A-stimulated Cultured Human Umbilical Vein Endothelial Cells J. Histochem. Cytochem. (IF 3.2) Pub Date : 2022-07-25 Esmeralda K. Bosma, Shahan Darwesh, Jia Y. Zheng, Cornelis J.F. van Noorden, Reinier O. Schlingemann, Ingeborg Klaassen
Endothelial cells (ECs) form a precisely regulated polarized monolayer in capillary walls. Vascular endothelial growth factor-A (VEGF-A) induces endothelial hyperpermeability, and VEGF-A applied to...
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Spatially Resolved and Highly Multiplexed Protein and RNA In Situ Detection by Combining CODEX With RNAscope In Situ Hybridization J. Histochem. Cytochem. (IF 3.2) Pub Date : 2022-07-16 Yilun Cheng, Rachel K. Burrack, Qingsheng Li
Highly multiplexed protein and RNA in situ detection on a single tissue section concurrently is highly desirable for both basic and applied biomedical research. CO-detection by inDEXing (CODEX) is ...
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Digital Staging of Hepatic Hemangiomas Reveals Spatial Heterogeneity in Endothelial Cell Composition and Vascular Senescence J. Histochem. Cytochem. (IF 3.2) Pub Date : 2022-07-09 Stefan Thomann, Marcell Tóth, Simon David Sprengel, Jakob Liermann, Peter Schirmacher
Hepatic hemangioma (HH) is the most common benign primary liver tumor; however, despite its high prevalence, a stage-specific classification of this tumor is currently missing. For a spatial stage-...
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Increased Colocalization and Interaction Between Decidual Protein Kinase A and Insulin-like Growth Factor–Binding Protein-1 in Intrauterine Growth Restriction J. Histochem. Cytochem. (IF 3.2) Pub Date : 2022-07-08 Madhulika B. Gupta, Kyle K. Biggar, Cun Li, Peter W. Nathanielsz, Thomas Jansson
Increased phosphorylation of decidual insulin-like growth factor–binding protein-1 (IGFBP-1) can contribute to intrauterine growth restriction (IUGR) by decreasing the bioavailability of insulin-li...
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The Relationship Between the Tumor Cell Expression of Hypoxic Markers and Survival in Patients With ER-positive Invasive Ductal Breast Cancer J. Histochem. Cytochem. (IF 3.2) Pub Date : 2022-07-06 Suad A.K. Shamis, Jean Quinn, Elizabeth E.A. Mallon, Joanne Edwards, Donald C. McMillan
The prognostic significance of hypoxia markers, hypoxia-inducible factor-1α (HIF-1α), hypoxia-inducible factor-2α (HIF-2α), and carbonic anhydrase IX (CAIX), was investigated in estrogen receptor (...
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Morphology of Schwann Cell Processes Supports Renal Sympathetic Nerve Terminals With Local Distribution of Adrenoceptors J. Histochem. Cytochem. (IF 3.2) Pub Date : 2022-06-16 Seishi Maeda, Yusuke Minato, Sachi Kuwahara-Otani, Hiroki Yamanaka, Mitsuyo Maeda, Yosky Kataoka, Hideshi Yagi
Nerves in the renal parenchyma comprise sympathetic nerves that act on renal arteries and tubules to decrease blood flow and increase primary urine reabsorption, respectively. Synaptic vesicles rel...