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ISEV2020 Abstract Book. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-08-17
(2020). ISEV2020 Abstract Book. Journal of Extracellular Vesicles: Vol. 9, ISEV2020: Abstract Book 2 (continued), 1806576.
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Towards defining reference materials for measuring extracellular vesicle refractive index, epitope abundance, size and concentration J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-09-24 Joshua A. Welsh; Edwin van der Pol; Britta A. Bettin; David R. F. Carter; An Hendrix; Metka Lenassi; Marc-André Langlois; Alicia Llorente; Arthur S. van de Nes; Rienk Nieuwland; Vera Tang; Lili Wang; Kenneth W. Witwer; Jennifer C. Jones
Accurate characterization of extracellular vesicles (EVs) is critical to explore their diagnostic and therapeutic applications. As the EV research field has developed, so too have the techniques used to characterize them. The development of reference materials are required for the standardization of these techniques. This work, initiated from the ISEV 2017 Biomarker Workshop in Birmingham, UK, and
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Fusion protein engineered exosomes for targeted degradation of specific RNAs in lysosomes: a proof-of-concept study J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-09-06 Zhelong Li; Xueying Zhou; Xiaotong Gao; Danna Bai; Yan Dong; Wenqi Sun; Lianbi Zhao; Mengying Wei; Xuekang Yang; Guodong Yang; Lijun Yuan
Therapeutically intervening the function of RNA in vivo remains a big challenge. We here developed an exosome-based strategy to deliver engineered RNA-binding protein for the purpose of recruiting specific RNA to the lysosomes for degradation. As a proof-of-principle study, RNA-binding protein HuR was fused to the C-terminus of Lamp2b, a membrane protein localized in both exosome and lysosome. The
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The future of Extracellular Vesicles as Theranostics – an ISEV meeting report J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-09-04 Carolina Soekmadji; Bo Li; Yiyao Huang; Haifang Wang; Taixue An; Chunchen Liu; Weilun Pan; Jing Chen; Lesley Cheung; Juan Manuel Falcon-Perez; Yong Song Gho; Harry B. Holthofer; Minh T. N. Le; Antonio Marcilla; Lorraine O’Driscoll; Faezeh Shekari; Tang Long Shen; Ana Claudia Torrecilhas; Xiaomei Yan; Fuquan Yang; Hang Yin; Yu Xiao; Zezhou Zhao; Xue Zou; Qian Wang; Lei Zheng
The utilization of extracellular vesicles (EVs) in clinical theranostics has rapidly advanced in the past decade. In November 2018, the International Society for Extracellular Vesicles (ISEV) held a workshop on “EVs in Clinical Theranostic”. Here, we report the conclusions of roundtable discussions on the current advancement in the analysis technologies and we provide some guidelines to researchers
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Internalization of trophoblastic small extracellular vesicles and detection of their miRNA cargo in P-bodies. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-08-28 Hui Li,Itziar Pinilla-Macua,Yingshi Ouyang,Elena Sadovsky,Kazuhiro Kajiwara,Alexander Sorkin,Yoel Sadovsky
Pregnancy is a unique situation, in which placenta-derived small extracellular vesicles (sEVs) may communicate with maternal and foetal tissues. While relevant to homoeostatic and pathological functions, the mechanisms underlying sEV entry and cargo handling in target cells remain largely unknown. Using fluorescently or luminescently labelled sEVs, derived from primary human placental trophoblasts
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Characterization of brain-derived extracellular vesicles reveals changes in cellular origin after stroke and enrichment of the prion protein with a potential role in cellular uptake. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-08-27 Santra Brenna,Hermann C Altmeppen,Behnam Mohammadi,Björn Rissiek,Florence Schlink,Peter Ludewig,Christoph Krisp,Hartmut Schlüter,Antonio Virgilio Failla,Carola Schneider,Markus Glatzel,Berta Puig,Tim Magnus
Extracellular vesicles (EVs) are important means of intercellular communication and a potent tool for regenerative therapy. In ischaemic stroke, transient blockage of a brain artery leads to a lack of glucose and oxygen in the affected brain tissue, provoking neuronal death by necrosis in the core of the ischaemic region. The fate of neurons in the surrounding penumbra region depends on the stimuli
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Extracellular vesicles from human iPSC-derived neural stem cells: miRNA and protein signatures, and anti-inflammatory and neurogenic properties. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-08-26 Raghavendra Upadhya,Leelavathi N Madhu,Sahithi Attaluri,Daniel Leite Góes Gitaí,Marisa R Pinson,Maheedhar Kodali,Geetha Shetty,Gabriele Zanirati,Smrithi Kumar,Bing Shuai,Susan T Weintraub,Ashok K Shetty
Grafting of neural stem cells (NSCs) derived from human induced pluripotent stem cells (hiPSCs) has shown promise for brain repair after injury or disease, but safety issues have hindered their clinical application. Employing nano-sized extracellular vesicles (EVs) derived from hiPSC-NSCs appears to be a safer alternative because they likely have similar neuroreparative properties as NSCs and are amenable
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Plasma extracellular vesicles detected by Single Molecule array technology as a liquid biopsy for colorectal cancer. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-08-26 Ping Wei,Fei Wu,Bin Kang,Xiaohua Sun,Fabienne Heskia,Alexandre Pachot,Ji Liang,Dawei Li
Circulating extracellular vesicles (EVs) were recognized as a promising source of diagnostic biomarker. However, there are limited studies published in this area, partly due to the limited number of detection platformscapable of detecting extracellular vesicles.In this study, extracellular vesicle immunoassays were developed using the Single Molecule array technology (SiMoa) and their clinical applications
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Experimental artefacts can lead to misattribution of bioactivity from soluble mesenchymal stem cell paracrine factors to extracellular vesicles. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-08-26 Thomas E Whittaker,Anika Nagelkerke,Valeria Nele,Ulrike Kauscher,Molly M Stevens
It has been demonstrated that some commonly used Extracellular Vesicle (EV) isolation techniques can lead to substantial contamination with non-EV factors. Whilst it has been established that this impacts the identification of biomarkers, the impact on apparent EV bioactivity has not been explored. Extracellular vesicles have been implicated as critical mediators of therapeutic human mesenchymal stem
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Salivary extracellular vesicles inhibit Zika virus but not SARS-CoV-2 infection. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-08-24 Carina Conzelmann,Rüdiger Groß,Min Zou,Franziska Krüger,André Görgens,Manuela O Gustafsson,Samir El Andaloussi,Jan Münch,Janis A Müller
Zika virus (ZIKV) is mainly transmitted via mosquitos, but human-to-human transmissions also occur. The virus is shed into various body fluids including saliva, which represents a possible source of viral transmission. Thus, we here explored whether human saliva affects ZIKV infectivity. We found that physiological concentrations of pooled saliva dose-dependently inhibit ZIKV infection of monkey and
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Quantification of extracellular vesicles in vitro and in vivo using sensitive bioluminescence imaging. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-08-21 Dhanu Gupta,Xiuming Liang,Svetlana Pavlova,Oscar P B Wiklander,Giulia Corso,Ying Zhao,Osama Saher,Jeremy Bost,Antje M Zickler,Andras Piffko,Cecile L Maire,Franz L Ricklefs,Oskar Gustafsson,Virginia Castilla Llorente,Manuela O Gustafsson,R Beklem Bostancioglu,Doste R Mamand,Daniel W Hagey,André Görgens,Joel Z Nordin,Samir El Andaloussi
ABSTRACT Extracellular vesicles (EVs) are naturally occurring nano-sized carriers that are secreted by cells and facilitate cell-to-cell communication by their unique ability to transfer biologically active cargo. Despite the pronounced increase in our understanding of EVs over the last decade, from disease pathophysiology to therapeutic drug delivery, improved molecular tools to track their therapeutic
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A combined "eat me/don't eat me" strategy based on extracellular vesicles for anticancer nanomedicine. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-08-19 Zakia Belhadj,Bing He,Hailiang Deng,Siyang Song,Hua Zhang,Xueqing Wang,Wenbing Dai,Qiang Zhang
ABSTRACT A long-term and huge challenge in nanomedicine is the substantial uptake and rapid clearance mediated by the mononuclear phagocyte system (MPS), which enormously hinders the development of nanodrugs. Inspired by the natural merits of extracellular vesicles, we therefore developed a combined “eat me/don’t eat me” strategy in an effort to achieve MPS escape and efficient drug delivery. Methodologically
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(Sub)populations of extracellular vesicles released by TNF-α -triggered human endothelial cells promote vascular inflammation and monocyte migration. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-08-11 Baharak Hosseinkhani,Nynke M S van den Akker,Daniel G M Molin,Luc Michiels
ABSTRACT Substantial research has been devoted to discovering the translational potential of extracellular vesicles (EV) as a reliable liquid biopsy in the diagnosis and monitoring of several life-affecting diseases, including chronic inflammatory diseases (CID). So far, the role of EV in the development of CID remains largely unknown due to the lack of specific tools to separate the disease-associated
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Human ESC-sEVs alleviate age-related bone loss by rejuvenating senescent bone marrow-derived mesenchymal stem cells. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-08-10 Liangzhi Gong,Bi Chen,Juntao Zhang,Yongjin Sun,Ji Yuan,Xin Niu,Guowen Hu,Yu Chen,Zongping Xie,Zhifeng Deng,Qing Li,Yang Wang
ABSTRACT Tissue-resident stem cell senescence leads to stem cell exhaustion, which is a major cause of physiological and pathological ageing. Stem cell-derived extracellular vesicles (SC-EVs) have been reported in preclinical studies to possess therapeutic potential for diverse diseases. However, whether SC-EVs can rejuvenate senescent tissue stem cells to prevent age-related disorders still remains
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Dendritic cell derived exosomes loaded with immunoregulatory cargo reprogram local immune responses and inhibit degenerative bone disease in vivo. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-08-07 Mahmoud Elashiry,Mohamed M Elashiry,Ranya Elsayed,Mythily Rajendran,Carol Auersvald,Rana Zeitoun,Mohammad H Rashid,Roxan Ara,Mohamed M Meghil,Yutao Liu,Ali S Arbab,Roger M Arce,Mark Hamrick,Mohammed Elsalanty,Marshall Brendan,Rafal Pacholczyk,Christopher W Cutler
ABSTRACT Chronic bone degenerative diseases represent a major threat to the health and well-being of the population, particularly those with advanced age. This study isolated exosomes (EXO), natural nano-particles, from dendritic cells, the “directors” of the immune response, to examine the immunobiology of DC EXO in mice, and their ability to reprogram immune cells responsible for experimental alveolar
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Increased small extracellular vesicle secretion after chemotherapy via upregulation of cholesterol metabolism in acute myeloid leukaemia. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-08-07 Chang-Sook Hong,Emily Jeong,Michael Boyiadzis,Theresa L Whiteside
ABSTRACT Most patients with acute myeloid leukaemia (AML) experience disease recurrence after chemotherapy largely due to the development of drug resistance. Small extracellular vesicles (sEVs) are known to play a significant role in leukaemia drug resistance by delivery of anti-apoptotic proteins and genes conferring resistance to recipient cells. sEV levels are elevated in AML patients’ plasma at
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Systematic review of extracellular vesicle-based treatments for lung injury: are EVs a potential therapy for COVID-19? J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-08-06 Kasra Khalaj,Rebeca Lopes Figueira,Lina Antounians,Giuseppe Lauriti,Augusto Zani
ABSTRACT Severe COVID-19 infection results in bilateral interstitial pneumonia, often leading to acute respiratory distress syndrome (ARDS) and pulmonary fibrosis in survivors. Most patients with severe COVID-19 infections who died had developed ARDS. Currently, ARDS is treated with supportive measures, but regenerative medicine approaches including extracellular vesicle (EV)-based therapies have shown
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Dendritic cells release exosomes together with phagocytosed pathogen; potential implications for the role of exosomes in antigen presentation. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-07-26 Marthe F S Lindenbergh,Richard Wubbolts,Ellen G F Borg,Esther M van 't Veld,Marianne Boes,W Stoorvogel
ABSTRACT Dendritic cells (DC) have the unique capacity to activate naïve T cells by presenting T cell receptor specific peptides from exogenously acquired antigens bound to Major Histocompatibility Complex (MHC) molecules. MHC molecules are displayed on the DC plasma membrane as well as on extracellular vesicles (EV) that are released by DC, and both have antigen-presenting capacities. However, the
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Study of immune-tolerized cell lines and extracellular vesicles inductive environment promoting continuous expression and secretion of HLA-G from semiallograft immune tolerance during pregnancy. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-07-20 Kyoungshik Cho,Hyejin Kook,Suman Kang,Jangho Lee
ABSTRACT An immune reaction is a protector of our body but a target to be overcome for all non-self-derived medicine. Extracellular Vesicles (EVs), noted as a primary alternative to cell therapy products that exhibit immune rejection due to mismatching-major histocompatibility complex (MHC), were discovered to have excellent curative effects through the delivery of various biologically active substances
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Lactobacillus plantarum-derived extracellular vesicles induce anti-inflammatory M2 macrophage polarization in vitro. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-07-17 Wanil Kim,Eun Jung Lee,Il-Hong Bae,Kilsun Myoung,Sung Tae Kim,Phil June Park,Kyung-Ha Lee,An Vuong Quynh Pham,Jaeyoung Ko,Sang Ho Oh,Eun-Gyung Cho
ABSTRACT Probiotics offer various health benefits. Lactobacillus plantarum has been used for decades to enhance human intestinal mucosal immunity and improve skin barrier integrity. Extracellular vesicles (EVs) derived from eukaryotic or prokaryotic cells have been recognized as efficient carriers for delivery of biomolecules to recipient cells, and to efficiently regulate human pathophysiology. However
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Cargo and cell-specific differences in extracellular vesicle populations identified by multiplexed immunofluorescent analysis. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-07-17 Kevin Burbidge,Virginia Zwikelmaier,Ben Cook,Michael M Long,Barak Balva,Michael Lonigro,Grace Ispas,David J Rademacher,Edward M Campbell
ABSTRACT Extracellular vesicles (EVs) have been implicated in a wide variety of biological activities, have been implicated in the pathogenesis of numerous diseases, and have been proposed to serve as potential biomarkers of disease in human patients and animal models. However, characterization of EV populations is often performed using methods that do not account for the heterogeneity of EV populations
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In vivo identification of apoptotic and extracellular vesicle-bound live cells using image-based deep learning. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-07-16 Jan Kranich,Nikolaos-Kosmas Chlis,Lisa Rausch,Ashretha Latha,Martina Schifferer,Tilman Kurz,Agnieszka Foltyn-Arfa Kia,Mikael Simons,Fabian J Theis,Thomas Brocker
ABSTRACT The in vivo detection of dead cells remains a major challenge due to technical hurdles. Here, we present a novel method, where injection of fluorescent milk fat globule-EGF factor 8 protein (MFG-E8) in vivo combined with imaging flow cytometry and deep learning allows the identification of dead cells based on their surface exposure of phosphatidylserine (PS) and other image parameters. A convolutional
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How does an RNA selfie work? EV-associated RNA in innate immunity as self or danger. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-07-15 Yu Xiao,Tom Driedonks,Kenneth W Witwer,Qian Wang,Hang Yin
ABSTRACT Innate immunity is a first line of defence against danger. Exogenous pathogen- or microbe-associated molecular patterns (PAMPs or MAMPs) trigger innate immune responses through well-understood cellular pathways. In contrast, endogenous damage-associated molecular patterns (DAMPs) convey “danger signals” via their (mis)localization or modification. Both MAMPs and DAMPs are often communicated
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A novel three step protocol to isolate extracellular vesicles from plasma or cell culture medium with both high yield and purity. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-07-14 Xiaogang Zhang,Ellen G F Borg,A Manuel Liaci,Harmjan R Vos,Willem Stoorvogel
ABSTRACT Extracellular vesicles (EV) are membrane encapsulated nanoparticles that can function in intercellular communication, and their presence in biofluids can be indicative for (patho)physiological conditions. Studies aiming to resolve functionalities of EV or to discover EV-associated biomarkers for disease in liquid biopsies are hampered by limitations of current protocols to isolate EV from
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Metabolic characteristics of large and small extracellular vesicles from pleural effusion reveal biomarker candidates for the diagnosis of tuberculosis and malignancy. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-07-14 Ping Luo,Kaimin Mao,Juanjuan Xu,Feng Wu,Xuan Wang,Sufei Wang,Mei Zhou,Limin Duan,Qi Tan,Guangzhou Ma,Guanghai Yang,Ronghui Du,Hai Huang,Qi Huang,Yumei Li,Mengfei Guo,Yang Jin
ABSTRACT Pleural effusion is a common respiratory disease worldwide; however, rapid and accurate diagnoses of tuberculosis pleural effusion (TPE) and malignancy pleural effusion (MPE) remain challenging. Although extracellular vesicles (EVs) have been confirmed as promising sources of disease biomarkers, little is known about the metabolite compositions of its subpopulations and their roles in the
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miRNA- and cytokine-associated extracellular vesicles mediate squamous cell carcinomas J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-07-13 Joseph P. Flemming; Brianna L. Hill; Mohammed W. Haque; Jessica Raad; Claudine S. Bonder; Larry A. Harshyne; Ulrich Rodeck; Adam Luginbuhl; James K. Wahl III; Kenneth Y. Tsai; Peter J. Wermuth; Andrew M. Overmiller; Mỹ G. Mahoney
ABSTRACT Exosomes, or small extracellular vesicles (sEVs), serve as intercellular messengers with key roles in normal and pathological processes. Our previous work had demonstrated that Dsg2 expression in squamous cell carcinoma (SCC) cells enhanced both sEV secretion and loading of pro-mitogenic cargo. In this study, using wild-type Dsg2 and a mutant form that is unable to be palmitoylated (Dsg2cacs)
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Mesenchymal stromal cell-derived small extracellular vesicles restore lung architecture and improve exercise capacity in a model of neonatal hyperoxia-induced lung injury. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-07-13 Gareth R Willis,Angeles Fernandez-Gonzalez,Monica Reis,Vincent Yeung,Xianlan Liu,Maria Ericsson,Nick A Andrews,S Alex Mitsialis,Stella Kourembanas
ABSTRACT Early administration of mesenchymal stromal cell (MSC)-derived small extracellular vesicles (MEx) has shown considerable promise in experimental models of bronchopulmonary dysplasia (BPD). However, the ability of MEx to reverse the long-term pulmonary complications associated with established BPD remains unknown. In this study, MEx were isolated from media conditioned by human Wharton’s Jelly-derived
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miRNA- and cytokine-associated extracellular vesicles mediate squamous cell carcinomas. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-07-13 Joseph P Flemming,Brianna L Hill,Mohammed W Haque,Jessica Raad,Claudine S Bonder,Larry A Harshyne,Ulrich Rodeck,Adam Luginbuhl,James K Wahl,Kenneth Y Tsai,Peter J Wermuth,Andrew M Overmiller,Mỹ G Mahoney
Exosomes, or small extracellular vesicles (sEVs), serve as intercellular messengers with key roles in normal and pathological processes. Our previous work had demonstrated that Dsg2 expression in squamous cell carcinoma (SCC) cells enhanced both sEV secretion and loading of pro-mitogenic cargo. In this study, using wild-type Dsg2 and a mutant form that is unable to be palmitoylated (Dsg2cacs), we investigated
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Sja-miR-71a in Schistosome egg-derived extracellular vesicles suppresses liver fibrosis caused by schistosomiasis via targeting semaphorin 4D. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-07-09 Lifu Wang,Yao Liao,Ruibing Yang,Zilong Yu,Lichao Zhang,Zifeng Zhu,Xiaoying Wu,Jia Shen,Jiahua Liu,Lian Xu,Zhongdao Wu,Xi Sun
ABSTRACT Schistosomiasis is characterized by liver fibrosis, and studies have indicated that Schistosoma japonicum (S. japonicum) eggs can limit the progression of liver fibrosis. However, the detailed molecular mechanisms are yet unclear. Extracellular vesicles (EVs) contain a selection of miRNAs for long-distance exchange of information and act as an important pathway for host-parasite communication
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The RNA-binding protein hnRNPU regulates the sorting of microRNA-30c-5p into large extracellular vesicles. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-07-02 Andreas Zietzer,Mohammed Rabiul Hosen,Han Wang,Philip Roger Goody,Marc Sylvester,Eicke Latz,Georg Nickenig,Nikos Werner,Felix Jansen
ABSTRACT The transfer of microRNAs (miRs) via extracellular vesicles (EVs) is a functionally relevant mechanism of intercellular communication that regulates both organ homoeostasis and disease development. Little is known about the packaging of miRs into EVs. Previous studies have shown that certain miRs are exported by RNA-binding proteins into small EVs, while for other miRs and for large EVs, in
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Influence of species and processing parameters on recovery and content of brain tissue-derived extracellular vesicles. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-06-30 Yiyao Huang,Lesley Cheng,Andrey Turchinovich,Vasiliki Mahairaki,Juan C Troncoso,Olga Pletniková,Norman J Haughey,Laura J Vella,Andrew F Hill,Lei Zheng,Kenneth W Witwer
ABSTRACT Extracellular vesicles (EVs) are involved in a wide range of physiological and pathological processes by shuttling material out of and between cells. Tissue EVs may thus lend insights into disease mechanisms and also betray disease when released into easily accessed biological fluids. Since brain-derived EVs (bdEVs) and their cargo may serve as biomarkers of neurodegenerative diseases, we
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Stumbling on elusive cargo: how isomiRs challenge microRNA detection and quantification, the case of extracellular vesicles. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-06-28 S Eréndira Avendaño-Vázquez,C Fabián Flores-Jasso
(2020). Stumbling on elusive cargo: how isomiRs challenge microRNA detection and quantification, the case of extracellular vesicles. Journal of Extracellular Vesicles: Vol. 9, No. 1, 1784617.
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Exosome therapeutics for lung regenerative medicine. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-06-29 Kristen Popowski,Halle Lutz,Shiqi Hu,Arianna George,Phuong-Uyen Dinh,Ke Cheng
ABSTRACT Exosomes are 30 to 100 nm extracellular vesicles that are secreted by many cell types. Initially viewed as cellular garbage with no biological functions, exosomes are now recognized for their therapeutic potential and used in regenerative medicine. Cell-derived exosomes are released into almost all biological fluids, making them abundant and accessible vesicles for a variety of diseases. These
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Stem cell-derived extracellular vesicles mitigate ageing-associated arterial stiffness and hypertension. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-06-24 Rui Feng,Mujib Ullah,Kai Chen,Quaisar Ali,Yi Lin,Zhongjie Sun
ABSTRACT The prevalence of arterial stiffness and hypertension increases with age. This study investigates the effect of induced pluripotent mesenchymal stem cell-derived extracellular vesicles (EVs) on ageing-associated arterial stiffness and hypertension. EVs were collected and purified from induced pluripotent stem cell-derived mesenchymal stem cells (iPS-MSCs). Young and old male C57BL/6 mice were
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Design of experiment (DoE)-driven in vitro and in vivo uptake studies of exosomes for pancreatic cancer delivery enabled by copper-free click chemistry-based labelling. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-06-19 Lizhou Xu,Farid N Faruqu,Revadee Liam-Or,Omar Abu Abed,Danyang Li,Kerrie Venner,Rachel J Errington,Huw Summers,Julie Tzu-Wen Wang,Khuloud T Al-Jamal
ABSTRACT Exosomes (Exo)-based therapy holds promise for treatment of lethal pancreatic cancer (PC). Limited understanding of key factors affecting Exo uptake in PC cells restricts better design of Exo-based therapy. This work aims to study the uptake properties of different Exo by PC cells. Exo from pancreatic carcinoma, melanoma and non-cancer cell lines were isolated and characterised for yield,
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Actin reorganization at the centrosomal area and the immune synapse regulates polarized secretory traffic of multivesicular bodies in T lymphocytes. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-06-19 Ana Bello-Gamboa,Marta Velasco,Solange Moreno,Gonzalo Herranz,Roxana Ilie,Silvia Huetos,Sergio Dávila,Alicia Sánchez,Jorge Bernardino De La Serna,Víctor Calvo,Manuel Izquierdo
ABSTRACT T-cell receptor stimulation induces the convergence of multivesicular bodies towards the microtubule-organizing centre (MTOC) and the polarization of the MTOC to the immune synapse (IS). These events lead to exosome secretion at the IS. We describe here that upon IS formation centrosomal area F-actin decreased concomitantly with MTOC polarization to the IS. PKCδ-interfered T cell clones showed
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Endothelial cell-derived extracellular vesicles alter vascular smooth muscle cell phenotype through high-mobility group box proteins. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-06-18 Michael J Boyer,Yayoi Kimura,Tomoko Akiyama,Ariele Y Baggett,Kyle J Preston,Rosario Scalia,Satoru Eguchi,Victor Rizzo
ABSTRACT The vascular endothelium and smooth muscle form adjacent cellular layers that comprise part of the vascular wall. Each cell type can regulate the other’s structure and function through a variety of paracrine effectors. Extracellular vesicles (EVs) are released from and transit between cells constituting a novel means of cell–cell communication. Here, we characterized the proteome of EVs released
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miR-23a-3p-abundant small extracellular vesicles released from Gelma/nanoclay hydrogel for cartilage regeneration. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-06-16 Hongxing Hu,Lanlan Dong,Ziheng Bu,Yifan Shen,Jian Luo,Hang Zhang,Shichang Zhao,Fang Lv,Zhongtang Liu
ABSTRACT Articular cartilage has limited self-regenerative capacity and the therapeutic methods for cartilage defects are still dissatisfactory in clinic. Recent studies showed that exosomes derived from mesenchymal stem cells promoted chondrogenesis by delivering bioactive substances to the recipient cells, indicating exosomes might be a novel method for repairing cartilage defect. Herein, we investigated
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Comprehensive palmitoyl-proteomic analysis identifies distinct protein signatures for large and small cancer-derived extracellular vesicles. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-06-10 Javier Mariscal,Tatyana Vagner,Minhyung Kim,Bo Zhou,Andrew Chin,Mandana Zandian,Michael R Freeman,Sungyong You,Andries Zijlstra,Wei Yang,Dolores Di Vizio
ABSTRACT Extracellular vesicles (EVs) are membrane-enclosed particles that play an important role in cancer progression and have emerged as a promising source of circulating biomarkers. Protein S-acylation, frequently called palmitoylation, has been proposed as a post-translational mechanism that modulates the dynamics of EV biogenesis and protein cargo sorting. However, technical challenges have limited
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Small RNA fingerprinting of Alzheimer's disease frontal cortex extracellular vesicles and their comparison with peripheral extracellular vesicles. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-06-04 Lesley Cheng,Laura J Vella,Kevin J Barnham,Catriona McLean,Colin L Masters,Andrew F Hill
ABSTRACT Alzheimer’s disease is a progressive neurodegenerative disorder, with the strongest disease-associated changes observed at clinical or end-stage disease. Transcriptomic deregulation of miRNA expression can spread via “horizontal” RNA transfer through extracellular vesicles (EVs) to act in conjunction with proteins, leading to changes in mRNA, which can provide early signals to indicate forthcoming
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Neuroblastoma-secreted exosomes carrying miR-375 promote osteogenic differentiation of bone-marrow mesenchymal stromal cells. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-06-03 Marta Colletti,Luigi Tomao,Angela Galardi,Alessandro Paolini,Virginia Di Paolo,Cristiano De Stefanis,Paolo Mascio,Francesca Nazio,Stefania Petrini,Aurora Castellano,Ida Russo,Roberta Caruso,Simone Piga,Rita De Vito,Luisa Pascucci,Hector Peinado,Andrea Masotti,Franco Locatelli,Angela Di Giannatale
ABSTRACT Bone marrow (BM) is the major target organ for neuroblastoma (NB) metastasis and its involvement is associated with poor outcome. Yet, the mechanism by which NB cells invade BM is largely unknown. Tumour microenvironment represents a key element in tumour progression and mesenchymal stromal cells (MSCs) have been recognized as a fundamental part of the associated tumour stroma. Here, we show
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Triple knockdown of CDC37, HSP90-alpha and HSP90-beta diminishes extracellular vesicles-driven malignancy events and macrophage M2 polarization in oral cancer J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-05-31 Kisho Ono; Chiharu Sogawa; Hotaka Kawai; Manh Tien Tran; Eman A. Taha; Yanyin Lu; May Wathone Oo; Yuka Okusha; Hirohiko Okamura; Soichiro Ibaragi; Masaharu Takigawa; Ken-Ichi Kozaki; Hitoshi Nagatsuka; Akira Sasaki; Kuniaki Okamoto; Stuart K. Calderwood; Takanori Eguchi
ABSTRACT Evidence has been accumulating to indicate that extracellular vesicles (EVs), including exosomes, released by cancer cells can foster tumour progression. The molecular chaperones – CDC37, HSP90α and HSP90β play key roles in cancer progression including epithelial-mesenchymal transition (EMT), although their contribution to EVs-mediated cell–cell communication in tumour microenvironment has
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Y-RNA subtype ratios in plasma extracellular vesicles are cell type- specific and are candidate biomarkers for inflammatory diseases. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-05-26 Tom A P Driedonks,Sanne Mol,Sanne de Bruin,Anna-Linda Peters,Xiaogang Zhang,Marthe F S Lindenbergh,Boukje M Beuger,Anne-Marieke D van Stalborch,Thom Spaan,Esther C de Jong,Erhard van der Vries,Coert Margadant,Robin van Bruggen,Alexander P J Vlaar,Tom Groot Kormelink,Esther N M Nolte-'t Hoen
Major efforts are made to characterize the presence of microRNA (miRNA) and messenger RNA in blood plasma to discover novel disease-associated biomarkers. MiRNAs in plasma are associated to several types of macromolecular structures, including extracellular vesicles (EV), lipoprotein particles (LPP) and ribonucleoprotein particles (RNP). RNAs in these complexes are recovered at variable efficiency
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Y-RNA subtype ratios in plasma extracellular vesicles are cell type- specific and are candidate biomarkers for inflammatory diseases J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-05-26 Tom A.P. Driedonks; Sanne Mol; Sanne de Bruin; Anna-Linda Peters; Xiaogang Zhang; Marthe F.S. Lindenbergh; Boukje M. Beuger; Anne-Marieke D. van Stalborch; Thom Spaan; Esther C. de Jong; Erhard van der Vries; Coert Margadant; Robin van Bruggen; Alexander P.J. Vlaar; Tom Groot Kormelink; Esther N.M. Nolte-‘T Hoen
ABSTRACT Major efforts are made to characterize the presence of microRNA (miRNA) and messenger RNA in blood plasma to discover novel disease-associated biomarkers. MiRNAs in plasma are associated to several types of macromolecular structures, including extracellular vesicles (EV), lipoprotein particles (LPP) and ribonucleoprotein particles (RNP). RNAs in these complexes are recovered at variable efficiency
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Small extracellular vesicles modulated by αVβ3 integrin induce neuroendocrine differentiation in recipient cancer cells. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-05-24 Fabio Quaglia,Shiv Ram Krishn,George G Daaboul,Srawasti Sarker,Raffaella Pippa,Josep Domingo-Domenech,Gaurav Kumar,Paolo Fortina,Peter McCue,William K Kelly,Himisha Beltran,Qin Liu,Lucia R Languino
ABSTRACT The ability of small extracellular vesicles (sEVs) to reprogram cancer cells is well established. However, the specific sEV components able to mediate aberrant effects in cancer cells have not been characterized. Integrins are major players in mediating sEV functions. We have previously reported that the αVβ3 integrin is detected in sEVs of prostate cancer (PrCa) cells and transferred into
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Monitoring HSP70 exosomes in cancer patients' follow up: a clinical prospective pilot study. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-05-20 Gaëtan Chanteloup,Marine Cordonnier,Nicolas Isambert,Aurélie Bertaut,Alice Hervieu,Audrey Hennequin,Maxime Luu,Sylvie Zanetta,Bruno Coudert,Leila Bengrine,Isabelle Desmoulins,Laure Favier,Aurélie Lagrange,Pierre-Benoit Pages,Ivan Gutierrez,Jeanine Lherminier,Laure Avoscan,Clémentine Jankowski,Cédric Rébé,Angélique Chevriaux,Marie-Martine Padeano,Charles Coutant,Sylvain Ladoire,Sylvain Causeret,Laurent
Exosomes are nanovesicles released by all cells that can be found in the blood. A key point for their use as potential biomarkers in cancer is to differentiate tumour-derived exosomes from other circulating nanovesicles. Heat shock protein-70 (HSP70) has been shown to be abundantly expressed by cancer cells and to be associated with bad prognosis. We previously showed that exosomes derived from cancer
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The αvβ6 integrin in cancer cell-derived small extracellular vesicles enhances angiogenesis. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-05-20 Shiv Ram Krishn,Israa Salem,Fabio Quaglia,Nicole M Naranjo,Ekta Agarwal,Qin Liu,Srawasti Sarker,Jessica Kopenhaver,Peter A McCue,Paul H Weinreb,Shelia M Violette,Dario C Altieri,Lucia R Languino
ABSTRACT Prostate cancer (PrCa) cells crosstalk with the tumour microenvironment by releasing small extracellular vesicles (sEVs). sEVs, as well as large extracellular vesicles (LEVs), isolated via iodixanol density gradients from PrCa cell culture media, express the epithelial-specific αvβ6 integrin, which is known to be induced in cancer. In this study, we show sEV-mediated protein transfer of αvβ6
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Monitoring HSP70 exosomes in cancer patients’ follow up: a clinical prospective pilot study J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-05-20 Gaëtan Chanteloup; Marine Cordonnier; Nicolas Isambert; Aurélie Bertaut; Alice Hervieu; Audrey Hennequin; Maxime Luu; Sylvie Zanetta; Bruno Coudert; Leila Bengrine; Isabelle Desmoulins; Laure Favier; Aurélie Lagrange; Pierre-Benoit Pages; Ivan Gutierrez; Jeanine Lherminier; Laure Avoscan; Clémentine Jankowski; Cédric Rébé; Angélique Chevriaux; Marie-Martine Padeano; Charles Coutant; Sylvain Ladoire;
ABSTRACT Exosomes are nanovesicles released by all cells that can be found in the blood. A key point for their use as potential biomarkers in cancer is to differentiate tumour-derived exosomes from other circulating nanovesicles. Heat shock protein-70 (HSP70) has been shown to be abundantly expressed by cancer cells and to be associated with bad prognosis. We previously showed that exosomes derived
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Indoor dust extracellular vesicles promote cancer lung metastasis by inducing tumour necrosis factor-α. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-05-19 Nhung Thi Hong Dinh,Jaewook Lee,Jaemin Lee,Sang Soo Kim,Gyeongyun Go,Seoyoon Bae,Ye In Jun,Yae Jin Yoon,Tae-Young Roh,Yong Song Gho
Indoor pollutants are important problems to public health. Among indoor pollutants, indoor dust contains extracellular vesicles (EVs), which are associated with pulmonary inflammation. However, it has not been reported whether indoor dust EVs affect the cancer lung metastasis. In this study, we isolated indoor dust EVs and investigated their roles in cancer lung metastasis. Upon intranasal administration
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Indoor dust extracellular vesicles promote cancer lung metastasis by inducing tumour necrosis factor-α J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-05-19 Nhung Thi Hong Dinh; Jaewook Lee; Jaemin Lee; Sang Soo Kim; Gyeongyun Go; Seoyoon Bae; Ye In Jun; Yae Jin Yoon; Tae-Young Roh; Yong Song Gho
ABSTRACT Indoor pollutants are important problems to public health. Among indoor pollutants, indoor dust contains extracellular vesicles (EVs), which are associated with pulmonary inflammation. However, it has not been reported whether indoor dust EVs affect the cancer lung metastasis. In this study, we isolated indoor dust EVs and investigated their roles in cancer lung metastasis. Upon intranasal
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CD73+ extracellular vesicles inhibit angiogenesis through adenosine A2B receptor signalling. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-05-04 Roberta Angioni,Cristina Liboni,Stephanie Herkenne,Ricardo Sánchez-Rodríguez,Giulia Borile,Elisabetta Marcuzzi,Bianca Calì,Maurizio Muraca,Antonella Viola
Pathological angiogenesis is a hallmark of several conditions including eye diseases, inflammatory diseases, and cancer. Stromal cells play a crucial role in regulating angiogenesis through the release of soluble factors or direct contact with endothelial cells. Here, we analysed the properties of the extracellular vesicles (EVs) released by bone marrow mesenchymal stromal cells (MSCs) and explored
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CD73+ extracellular vesicles inhibit angiogenesis through adenosine A2B receptor signalling J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-05-04 Roberta Angioni; Cristina Liboni; Stephanie Herkenne; Ricardo Sánchez-Rodríguez; Giulia Borile; Elisabetta Marcuzzi; Bianca Calì; Maurizio Muraca; Antonella Viola
ABSTRACT Pathological angiogenesis is a hallmark of several conditions including eye diseases, inflammatory diseases, and cancer. Stromal cells play a crucial role in regulating angiogenesis through the release of soluble factors or direct contact with endothelial cells. Here, we analysed the properties of the extracellular vesicles (EVs) released by bone marrow mesenchymal stromal cells (MSCs) and
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DC-SIGN mediated internalisation of glycosylated extracellular vesicles from Schistosoma mansoni increases activation of monocyte-derived dendritic cells. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-04-30 Marije E Kuipers,Esther N M Nolte-'t Hoen,Alwin J van der Ham,Arifa Ozir-Fazalalikhan,D Linh Nguyen,Clarize M de Korne,Roman I Koning,John J Tomes,Karl F Hoffmann,Hermelijn H Smits,Cornelis H Hokke
Helminths like Schistosoma mansoni release excretory/secretory (E/S) products that modulate host immunity to enable infection. Extracellular vesicles (EVs) are among these E/S products, yet molecular mechanisms and functionality of S. mansoni EV interaction with host immune cells is unknown. Here we demonstrate that EVs released by S. mansoni schistosomula are internalised by human monocyte-derived
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DC-SIGN mediated internalisation of glycosylated extracellular vesicles from Schistosoma mansoni increases activation of monocyte-derived dendritic cells J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-04-30 Marije E. Kuipers; Esther N.M. Nolte-‘t Hoen; Alwin J. van der Ham; Arifa Ozir-Fazalalikhan; D. Linh Nguyen; Clarize M. de Korne; Roman I. Koning; John J. Tomes; Karl F. Hoffmann; Hermelijn H. Smits; Cornelis H. Hokke
ABSTRACT Helminths like Schistosoma mansoni release excretory/secretory (E/S) products that modulate host immunity to enable infection. Extracellular vesicles (EVs) are among these E/S products, yet molecular mechanisms and functionality of S. mansoni EV interaction with host immune cells is unknown. Here we demonstrate that EVs released by S. mansoni schistosomula are internalised by human monocyte-derived
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Quantitative proteomic analysis of trypsin-treated extracellular vesicles to identify the real-vesicular proteins. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-04-30 Dongsic Choi,Gyeongyun Go,Dae-Kyum Kim,Jaewook Lee,Seon-Min Park,Dolores Di Vizio,Yong Song Gho
ABSTRACT Extracellular vesicles (EVs) are nano-sized vesicles surrounded by a lipid bilayer and released into the extracellular milieu by most of cells. Although various EV isolation methods have been established, most of the current methods isolate EVs with contaminated non-vesicular proteins. By applying the label-free quantitative proteomic analyses of human colon cancer cell SW480-derived EVs,
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Exosomes derived from neural progenitor cells preserve photoreceptors during retinal degeneration by inactivating microglia J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-04-21 Baishijiao Bian; Congjian Zhao; Xiangyu He; Yu Gong; Chunge Ren; Lingling Ge; Yuxiao Zeng; Qiyou Li; Min Chen; Chuanhuang Weng; Juncai He; Yajie Fang; Haiwei Xu; Zheng Qin Yin
ABSTRACT Retinal degeneration (RD) is one of the most common causes of visual impairment and blindness and is characterized by progressive degeneration of photoreceptors. Transplantation of neural stem/progenitor cells (NPCs) is a promising treatment for RD, although the mechanisms underlying the efficacy remain unclear. Accumulated evidence supports the notion that paracrine effects of transplanted
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Exosomes derived from neural progenitor cells preserve photoreceptors during retinal degeneration by inactivating microglia. J. Extracell. Vesicles (IF 14.976) Pub Date : 2020-04-21 Baishijiao Bian,Congjian Zhao,Xiangyu He,Yu Gong,Chunge Ren,Lingling Ge,Yuxiao Zeng,Qiyou Li,Min Chen,Chuanhuang Weng,Juncai He,Yajie Fang,Haiwei Xu,Zheng Qin Yin
Retinal degeneration (RD) is one of the most common causes of visual impairment and blindness and is characterized by progressive degeneration of photoreceptors. Transplantation of neural stem/progenitor cells (NPCs) is a promising treatment for RD, although the mechanisms underlying the efficacy remain unclear. Accumulated evidence supports the notion that paracrine effects of transplanted stem cells