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Proteomic analysis of ascitic extracellular vesicles describes tumour microenvironment and predicts patient survival in ovarian cancer J. Extracell. Vesicles (IF 16.0) Pub Date : 2024-03-15 Anna Vyhlídalová Kotrbová, Kristína Gömöryová, Antónia Mikulová, Hana Plešingerová, Stanislava Sladeček, Marek Kravec, Šárka Hrachovinová, David Potěšil, Garett Dunsmore, Camille Blériot, Mathilde Bied, Jan Kotouček, Markéta Bednaříková, Jitka Hausnerová, Luboš Minář, Igor Crha, Michal Felsinger, Zbyněk Zdráhal, Florent Ginhoux, Vít Weinberger, Vitězslav Bryja, Vendula Pospíchalová
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Correction to “The dark side of foetal bovine serum in extracellular vesicle studies” J. Extracell. Vesicles (IF 16.0) Pub Date : 2024-03-15
Urzì, O., Olofsson Bagge, R., & Crescitelli, R. (2022). The dark side of foetal bovine serum in extracellular vesicle studies. Journal of Extracellular Vesicles, 11, e12271. https://doi.org/10.1002/jev2.12271 In the originally published article, the year in the received/revised/accepted dates was incorrectly given as 1912. This has been corrected in the online version of the article. The citation has
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PD-L1 on large extracellular vesicles is a predictive biomarker for therapy response in tissue PD-L1-low and -negative patients with non-small cell lung cancer J. Extracell. Vesicles (IF 16.0) Pub Date : 2024-03-07 Nadja Schöne, Marcel Kemper, Kerstin Menck, Georg Evers, Carolin Krekeler, Arik Bernard Schulze, Georg Lenz, Eva Wardelmann, Claudia Binder, Annalen Bleckmann
Immunotherapy has revolutionized the treatment of patients with non-small cell lung cancer (NSCLC). High expression of tissue PD-L1 (tPD-L1) is currently the only approved biomarker for predicting treatment response. However, even tPD-L1 low (1-49%) and absent (<1%) patients might benefit from immunotherapy but, to date, there is no reliable biomarker, that can predict response in this particular patient
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Extracellular vesicles as a promising source of lipid biomarkers for breast cancer detection in blood plasma J. Extracell. Vesicles (IF 16.0) Pub Date : 2024-03-05 Erika Dorado, Maria Luisa Doria, Anika Nagelkerke, James S. McKenzie, Stefania Maneta-Stavrakaki, Thomas E. Whittaker, Jeremy K. Nicholson, Raoul Charles Coombes, Molly M. Stevens, Zoltan Takats
Extracellular vesicles (EVs), including exosomes and microvesicles, mediate intercellular communication in cancer, from development to metastasis. EV-based liquid biopsy is a promising strategy for cancer diagnosis as EVs can be found in cancer patients’ body fluids. In this study, the lipid composition of breast cancer-derived EVs was studied as well as the potential of blood plasma EVs for the identification
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MISEV2023: An updated guide to EV research and applications J. Extracell. Vesicles (IF 16.0) Pub Date : 2024-02-23 Joshua A. Welsh, Deborah C. Goberdhan, Lorraine O'Driscoll, Clotilde Théry, Kenneth W. Witwer
1 MISEV2023: DELIVERING THE GOODS MISEV2023 has been written and revised to stand on its own as a guide to EV studies . Starting with an introduction with a historical perspective and ‘user guide’, MISEV2023 next takes the reader through all of the main aspects of an EV study. Community-sourced, it has something to offer beginners and veterans alike. The nomenclature section introduces the diversity
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Publishing the MISEV guidelines; The editorial process J. Extracell. Vesicles (IF 16.0) Pub Date : 2024-02-15 Jan Lötvall
Extracellular vesicle (EV) research has expanded exponentially over the last 10–15 years, with less than 100 entries in PubMed in 2007 but almost 8000 in 2022, depending on the specifics of the search terms applied. As this meteoric rise began, the field moved at a high pace, with methodology and technology evolving swiftly. This evolution has had notable impact on the stringency of the research produced
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Lipid profile of circulating placental extracellular vesicles during pregnancy identifies foetal growth restriction risk J. Extracell. Vesicles (IF 16.0) Pub Date : 2024-02-14 Miira M. Klemetti, Ante B. V. Pettersson, Aafaque Ahmad Khan, Leonardo Ermini, Tyler R. Porter, Michael L. Litvack, Sruthi Alahari, Stacy Zamudio, Nicholas P. Illsley, Hannes Röst, Martin Post, Isabella Caniggia
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Bioengineered small extracellular vesicles deliver multiple SARS-CoV-2 antigenic fragments and drive a broad immunological response J. Extracell. Vesicles (IF 16.0) Pub Date : 2024-02-09 Hannah K. Jackson, Heather M. Long, Juan Carlos Yam-Puc, Roberta Palmulli, Tracey A. Haigh, Pehuén Pereyra Gerber, Jin S. Lee, Nicholas J. Matheson, Lesley Young, John Trowsdale, Mathew Lo, Graham S. Taylor, James E. Thaventhiran, James R. Edgar
The COVID-19 pandemic highlighted the clear risk that zoonotic viruses pose to global health and economies. The scientific community responded by developing several efficacious vaccines which were expedited by the global need for vaccines. The emergence of SARS-CoV-2 breakthrough infections highlights the need for additional vaccine modalities to provide stronger, long-lived protective immunity. Here
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Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches J. Extracell. Vesicles (IF 16.0) Pub Date : 2024-02-07 Joshua A. Welsh, Deborah C. I. Goberdhan, Lorraine O'Driscoll, Edit I. Buzas, Cherie Blenkiron, Benedetta Bussolati, Houjian Cai, Dolores Di Vizio, Tom A. P. Driedonks, Uta Erdbrügger, Juan M. Falcon-Perez, Qing-Ling Fu, Andrew F. Hill, Metka Lenassi, Sai Kiang Lim, Mỹ G. Mahoney, Sujata Mohanty, Andreas Möller, Rienk Nieuwland, Takahiro Ochiya, Susmita Sahoo, Ana C. Torrecilhas, Lei Zheng, Andries
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in
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Identification of specific markers for human pluripotent stem cell-derived small extracellular vesicles J. Extracell. Vesicles (IF 16.0) Pub Date : 2024-02-06 Zhengsheng Chen, Lei Luo, Teng Ye, Jiacheng Zhou, Xin Niu, Ji Yuan, Ting Yuan, Dehao Fu, Haiyan Li, Qing Li, Yang Wang
Pluripotent stem cell-derived small extracellular vesicles (PSC-sEVs) have demonstrated great clinical translational potential in multiple aging-related degenerative diseases. Characterizing the PSC-sEVs is crucial for their clinical applications. However, the specific marker pattern of PSC-sEVs remains unknown. Here, the sEVs derived from two typical types of PSCs including induced pluripotent stem
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Tumour tissue-derived small extracellular vesicles reflect molecular subtypes of bladder cancer J. Extracell. Vesicles (IF 16.0) Pub Date : 2024-01-31 Liang Dong, Mingxiao Feng, Morgan D. Kuczler, Kengo Horie, Chi-Ju Kim, Zehua Ma, Kara Lombardo, Heather Lyons, Sarah R. Amend, Max Kates, Trinity J. Bivalacqua, David McConkey, Wei Xue, Woonyoung Choi, Kenneth J. Pienta
mRNA-based molecular subtypes have implications for bladder cancer prognosis and clinical benefit from certain therapies. Whether small extracellular vesicles (sEVs) can reflect bladder cancer molecular subtypes is unknown. We performed whole transcriptome RNA sequencing for formalin fixed paraffin embedded (FFPE) tumour tissues and sEVs separated from matched tissue explants, urine and plasma in patients
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Correction to Heat inactivation of foetal bovine serum performed after EV-depletion influences the proteome of cell-derived extracellular vesicles J. Extracell. Vesicles (IF 16.0) Pub Date : 2024-01-31
Urzì, O., Bergqvist, M., Lässer, C., Moschetti, M., Johansson, J., D´Arrigo, D., Olofsson Bagge, R., & Crescitelli, R. (2024). Heat inactivation of foetal bovine serum performed after EV-depletion influences the proteome of cell-derived extracellular vesicles. Journal of Extracellular Vesicles, 13, e12408. https://doi.org/10.1002/jev2.12408 In the originally published article, a portion of Figure 3
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Correction to “Recommendations for reproducibility of cerebrospinal fluid extracellular vesicle studies” J. Extracell. Vesicles (IF 16.0) Pub Date : 2024-01-24
Sandau, U. S., Magaña, S. M., Costa, J., Nolan, J. P., Ikezu, T., Vella, L. J., Jackson, H. K., Moreira, L. R., Palacio, P. L., Hill, A. F., Quinn, J. F., Van Keuren-Jensen, K. R., McFarland, T. J., Palade, J., Sribnick, E. A., Su, H., Vekrellis, K., Coyle, B., Yang, Y., … Saugstad, J. A., International Society for Extracellular Vesicles Cerebrospinal Fluid Task Force. (2024). Recommendations for reproducibility
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Heat inactivation of foetal bovine serum performed after EV-depletion influences the proteome of cell-derived extracellular vesicles J. Extracell. Vesicles (IF 16.0) Pub Date : 2024-01-23 Ornella Urzì, Markus Bergqvist, Cecilia Lässer, Marta Moschetti, Junko Johansson, Daniele D´Arrigo, Roger Olofsson Bagge, Rossella Crescitelli
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be influenced by cell culture conditions such as the presence of foetal bovine serum (FBS). Although several studies have evaluated the effect of removing FBS-derived EVs by ultracentrifugation (UC), less is known about the influence of FBS heat inactivation (HI) on the cell-derived EVs. To assess this
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Differential temporal release and lipoprotein loading in B. thetaiotaomicron bacterial extracellular vesicles J. Extracell. Vesicles (IF 16.0) Pub Date : 2024-01-19 Rokas Juodeikis, Carlo Martins, Gerhard Saalbach, Jake Richardson, Trey Koev, Dave J. Baker, Marianne Defernez, Martin Warren, Simon R. Carding
Bacterial extracellular vesicles (BEVs) contribute to stress responses, quorum sensing, biofilm formation and interspecies and interkingdom communication. However, the factors that regulate their release and heterogeneity are not well understood. We set out to investigate these factors in the common gut commensal Bacteroides thetaiotaomicron by studying BEV release throughout their growth cycle. Utilising
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Extracellular vesicles from periodontal pathogens regulate hepatic steatosis via Toll-like receptor 2 and plasminogen activator inhibitor-1 J. Extracell. Vesicles (IF 16.0) Pub Date : 2024-01-22 Hyun Young Kim, Younggap Lim, Ji Sun Jang, Yeon Kyeong Ko, Youngnim Choi, Hong-Hee Kim, Bong-Kyu Choi
Plasminogen activator inhibitor-1 (PAI-1) is associated with nonalcoholic fatty liver disease (NAFLD) by lipid accumulation in the liver. In this study, we showed that extracellular vesicles (EVs) from the periodontal pathogens Filifactor alocis and Porphyromonas gingivalis induced steatosis by inducing PAI-1 in the liver and serum of mice fed a low-fat diet. PAI-1 induction was not observed in TLR2−/−
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Extracellular vesicle-mediated delivery of CRISPR/Cas9 ribonucleoprotein complex targeting proprotein convertase subtilisin-kexin type 9 (Pcsk9) in primary mouse hepatocytes J. Extracell. Vesicles (IF 16.0) Pub Date : 2024-01-08 Nazma F. Ilahibaks, Thomas A. Kluiver, Olivier G. de Jong, Saskia C. A. de Jager, Raymond M. Schiffelers, Pieter Vader, Weng Chuan Peng, Zhiyong Lei, Joost P. G. Sluijter
The loss-of-function of the proprotein convertase subtilisin–kexin type 9 (Pcsk9) gene has been associated with significant reductions in plasma serum low-density lipoprotein cholesterol (LDL-C) levels. Both CRISPR/Cas9 and CRISPR-based editor-mediated Pcsk9 inactivation have successfully lowered plasma LDL-C and PCSK9 levels in preclinical models. Despite the promising preclinical results, these studies
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Novel method for collecting hippocampal interstitial fluid extracellular vesicles (EVISF) reveals sex-dependent changes in microglial EV proteome in response to Aβ pathology J. Extracell. Vesicles (IF 16.0) Pub Date : 2024-01-08 Morgan C. Pait, Sarah D. Kaye, Yixin Su, Ashish Kumar, Sangeeta Singh, Stephen C. Gironda, Samantha Vincent, Maria Anwar, Caitlin M. Carroll, James Andy Snipes, Jingyun Lee, Cristina M. Furdui, Gagan Deep, Shannon L. Macauley
Brain-derived extracellular vesicles (EVs) play an active role in Alzheimer's disease (AD), relaying important physiological information about their host tissues. The internal cargo of EVs is protected from degradation, making EVs attractive AD biomarkers. However, it is unclear how circulating EVs relate to EVs isolated from disease-vulnerable brain regions. We developed a novel method for collecting
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A beginner's guide to study extracellular vesicles in human blood plasma and serum J. Extracell. Vesicles (IF 16.0) Pub Date : 2024-01-09 Rienk Nieuwland, Pia R-M Siljander
Blood is the most commonly used body fluid for obtaining and studying extracellular vesicles (EVs). While blood is a standard choice for clinical analysis, using blood as a source of EVs introduces multiple layers of complexity. At the Blood Extracellular Vesicle Workshop organized by the International Society for Extracellular Vesicles in Helsinki (2022), it became evident that beginner researchers
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Size matters: Functional differences of small extracellular vesicle subpopulations in cardiac repair responses J. Extracell. Vesicles (IF 16.0) Pub Date : 2024-01-05 Simonides Immanuel van de Wakker, Julia Bauzá-Martinez, Carla Ríos Arceo, Herak Manjikian, Christian Jamie Bernard Snijders Blok, Marieke Theodora Roefs, Eduard Willms, Renee Goverdina Catharina Maas, Matti Feije Pronker, Olivier Gerrit de Jong, Wei Wu, André Görgens, Samir El Andaloussi, Joost Petrus Gerardus Sluijter, Pieter Vader
Cardiac progenitor cell (CPC)-derived small extracellular vesicles (sEVs) exhibit great potential to stimulate cardiac repair. However, the multifaceted nature of sEV heterogeneity presents a challenge in understanding the distinct mechanisms underlying their regenerative abilities. Here, a dual-step multimodal flowthrough and size-exclusion chromatography method was applied to isolate and separate
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Dysregulation of extracellular vesicle protein cargo in female myalgic encephalomyelitis/chronic fatigue syndrome cases and sedentary controls in response to maximal exercise J. Extracell. Vesicles (IF 16.0) Pub Date : 2024-01-03 Ludovic Giloteaux, Katherine A. Glass, Arnaud Germain, Carl J. Franconi, Sheng Zhang, Maureen R. Hanson
In healthy individuals, physical exercise improves cardiovascular health and muscle strength, alleviates fatigue and reduces the risk of chronic diseases. Although exercise is suggested as a lifestyle intervention to manage various chronic illnesses, it negatively affects people with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), who suffer from exercise intolerance. We hypothesized that
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Recommendations for reproducibility of cerebrospinal fluid extracellular vesicle studies J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-12-29 Ursula S. Sandau, Setty M. Magaña, Júlia Costa, John P. Nolan, Tsuneya Ikezu, Laura J. Vella, Hannah K. Jackson, Lissette Retana Moreira, Paola Loreto Palacio, Andrew F. Hill, Joseph F. Quinn, Kendall R. Van Keuren-Jensen, Trevor J. McFarland, Joanna Palade, Eric A. Sribnick, Huaqi Su, Kostas Vekrellis, Beth Coyle, You Yang, Juan M. Falcón-Perez, Rienk Nieuwland, Julie A. Saugstad
Cerebrospinal fluid (CSF) is a clear, transparent fluid derived from blood plasma that protects the brain and spinal cord against mechanical shock, provides buoyancy, clears metabolic waste and transports extracellular components to remote sites in the brain. Given its contact with the brain and the spinal cord, CSF is the most informative biofluid for studies of the central nervous system (CNS). In
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Extracellular vesicles from organoid-derived human retinal progenitor cells prevent lipid overload-induced retinal pigment epithelium injury by regulating fatty acid metabolism J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-12-27 Hui Gao, Yuxiao Zeng, Xiaona Huang, Luodan A, Qingle Liang, Jing Xie, Xi Lin, Jing Gong, Xiaotang Fan, Ting Zou, Haiwei Xu
Retinal degeneration (RD), a group of diseases leading to irreversible vision loss, is characterised by retinal pigment epithelium (RPE) or retinal neuron damage and loss. With fewer risks of immune rejection and tumorigenesis, stem cell-secreted extracellular vesicles (EVs) offer a new cell-free therapeutic paradigm for RD, which remains to be investigated. Human retinal organoid-derived retinal progenitor
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High-fat diets: You are what you eat….your extracellular vesicles too! J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-12-27 Sophie Rome, Stefano Tacconi
Recent works indicate that the lipid composition of extracellular vesicles (EVs) can modify their biological functions and their incorporation into recipient cells. In particular high-fat diets affect EV biogenesis, EV lipid composition, EV targeting and consequently the cross-talk between tissues. This review connects different research topics to show that a vicious circle is established during the
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Extracellular vesicle-associated cholesterol supports the regenerative functions of macrophages in the brain J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-12-20 Sam Vanherle, Jeroen Guns, Melanie Loix, Fleur Mingneau, Tess Dierckx, Flore Wouters, Koen Kuipers, Tim Vangansewinkel, Esther Wolfs, Paula Pincela Lins, Annelies Bronckaers, Ivo Lambrichts, Jonas Dehairs, Johannes V. Swinnen, Sanne G. S. Verberk, Mansour Haidar, Jerome J. A. Hendriks, Jeroen F. J. Bogie
Macrophages play major roles in the pathophysiology of various neurological disorders, being involved in seemingly opposing processes such as lesion progression and resolution. Yet, the molecular mechanisms that drive their harmful and benign effector functions remain poorly understood. Here, we demonstrate that extracellular vesicles (EVs) secreted by repair-associated macrophages (RAMs) enhance remyelination
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Extracellular vesicles derived from nasopharyngeal carcinoma induce the emergence of mature regulatory dendritic cells using a galectin-9 dependent mechanism J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-12-20 Anthony Lefebvre, Camille Trioën, Sarah Renaud, William Laine, Benjamin Hennart, Clément Bouchez, Bertrand Leroux, Delphine Allorge, Jérôme Kluza, Elisabeth Werkmeister, Guillaume Paul Grolez, Nadira Delhem, Olivier Moralès
Nasopharyngeal carcinoma-derived small extracellular vesicles (NPCSEVs) have an immunosuppressive impact on the tumour microenvironment. In this study, we investigated their influence on the generation of tolerogenic dendritic cells and the potential involvement of the galectin-9 (Gal9) they carry in this process. We analysed the phenotype and immunosuppressive properties of NPCSEVs and explored the
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Uptake of extracellular vesicles into immune cells is enhanced by the protein corona J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-12-20 Laura Dietz, Jennifer Oberländer, Ana Mateos-Maroto, Jenny Schunke, Michael Fichter, Eva-Maria Krämer-Albers, Katharina Landfester, Volker Mailänder
The influence of a protein corona on the uptake of nanoparticles in cells has been demonstrated in various publications over the last years. Extracellular vesicles (EVs), can be seen as natural nanoparticles. However, EVs are produced under different cell culture conditions and little is known about the protein corona forming on EVs and its influence on their uptake by target cells. Here, we use a
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Metabolic labelling of a subpopulation of small extracellular vesicles using a fluorescent palmitic acid analogue J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-12-10 Valeria Barreca, Zaira Boussadia, Deborah Polignano, Lorenzo Galli, Valentina Tirelli, Massimo Sanchez, Mario Falchi, Lucia Bertuccini, Francesca Iosi, Massimo Tatti, Massimo Sargiacomo, Maria Luisa Fiani
Exosomes are among the most puzzling vehicles of intercellular communication, but several crucial aspects of their biogenesis remain elusive, primarily due to the difficulty in purifying vesicles with similar sizes and densities. Here we report an effective methodology for labelling small extracellular vesicles (sEV) using Bodipy FL C16, a fluorescent palmitic acid analogue. In this study, we present
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Multiomic integration reveals neuronal-extracellular vesicle coordination of gliotic responses in degeneration J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-12-11 Adrian V. Cioanca, Yvette Wooff, Riemke Aggio-Bruce, Rakshanya Sekar, Catherine Dietrich, Riccardo Natoli
In the central nervous system (CNS), including in the retina, neuronal-to-glial communication is critical for maintaining tissue homeostasis including signal transmission, transfer of trophic factors, and in the modulation of inflammation. Extracellular vesicle (EV)-mediated transport of molecular messages to regulate these processes has been suggested as a mechanism by which bidirectional communication
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Proteomic comparison between non-purified cerebrospinal fluid and cerebrospinal fluid-derived extracellular vesicles from patients with Alzheimer's, Parkinson's and Lewy body dementia J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-12-11 Yael Hirschberg, Natalia Valle-Tamayo, Oriol Dols-Icardo, Sebastiaan Engelborghs, Bart Buelens, Roosmarijn E. Vandenbroucke, Yannick Vermeiren, Kurt Boonen, Inge Mertens
Dementia is a leading cause of death worldwide, with increasing prevalence as global life expectancy increases. The most common neurodegenerative disorders are Alzheimer's disease (AD), dementia with Lewy bodies (DLB) and Parkinson's disease dementia (PDD). With this study, we took an in-depth look at the proteome of the (non-purified) cerebrospinal fluid (CSF) and the CSF-derived extracellular vesicles
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Single-particle analysis of circulating bacterial extracellular vesicles reveals their biogenesis, changes in blood and links to intestinal barrier J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-12-05 Zihao Ou, Bo Situ, Xinyue Huang, Yicong Xue, Xiaojing He, Qianbei Li, Dejin Ou, Bairong He, Jing Chen, Yiyao Huang, Lulu Deng, Minying Zhang, Qian Wang, Lei Zheng
Bacterial extracellular vesicles (BEVs) are nano-size particles secreted by bacteria that carry various bioactive components. These vesicles are thought to provide a new window into the mechanisms by which bacteria affect their hosts, but their fundamental proprieties within human remain poorly understood. Here, we developed a single-vesicle analytical platform that enabled BEV detection in complex
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An ex vivo model of interactions between extracellular vesicles and peripheral mononuclear blood cells in whole blood J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-12-04 Blanca V. Rodriguez, Yi Wen, Erin N. Shirk, Samuel Vazquez, Olesia Gololobova, Amanda Maxwell, Jessica Plunkard, Natalie Castell, Bess Carlson, Suzanne E. Queen, Jessica M. Izzi, Tom A. P. Driedonks, Kenneth W. Witwer
Extracellular vesicles (EVs) can be loaded with therapeutic cargo and engineered for retention by specific body sites; therefore, they have great potential for targeted delivery of biomolecules to treat diseases. However, the pharmacokinetics and biodistribution of EVs in large animals remain relatively unknown, especially in primates. We recently reported that when cell culture-derived EVs are administered
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Correlation between membrane proteins and sizes of extracellular vesicles and particles: A potential signature for cancer diagnosis J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-12-05 Chunhui Zhai, Feng Xie, Jiaying Xu, Yuting Yang, Weiqiang Zheng, Haiyan Hu, Xianting Ding, Hui Yu
Extracellular vesicles and particles (EVPs) are recognized as ideal liquid biopsy tools for cancer detection, and membrane proteins are commonly used EVP biomarkers. However, bulk analysis of EVP membrane protein biomarkers typically fails to meet the clinical requirement for diagnostic accuracy. We investigated the correlation between the membrane protein expression level, the binding kinetics to
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Exploring the role of polymers to overcome ongoing challenges in the field of extracellular vesicles J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-12-05 Laurianne Simon, Vincent Lapinte, Marie Morille
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A clinically relevant large-scale biomanufacturing workflow to produce natural killer cells and natural killer cell-derived extracellular vesicles for cancer immunotherapy J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-12-06 Frederic St-Denis-Bissonnette, Sarah E. Cummings, Shirley Qiu, Andrew Stalker, Gauri Muradia, Jelica Mehic, Karan Mediratta, Shelby Kaczmarek, Dylan Burger, Seung-Hwan Lee, Lisheng Wang, Jessie R. Lavoie
Natural killer cell-derived extracellular vesicles (NK-EVs) have shown promising potential as biotherapeutics for cancer due to their unique attributes as cytotoxic nanovesicles against cancer cells and immune-modulatory activity towards immune cells. However, a biomanufacturing workflow is needed to produce clinical-grade NK-EVs for pre-clinical and clinical applications. This study established a
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MIBlood-EV: Minimal information to enhance the quality and reproducibility of blood extracellular vesicle research J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-12-08 Fabrice Lucien, Dakota Gustafson, Metka Lenassi, Bo Li, Jacob J. Teske, Eric Boilard, Katharina Clemm von Hohenberg, Juan Manual Falcón-Perez, Alice Gualerzi, Antonia Reale, Jennifer C. Jones, Cecilia Lässer, Charlotte Lawson, Irina Nazarenko, Lorraine O'Driscoll, Ryan Pink, Pia R-M Siljander, Carolina Soekmadji, An Hendrix, Joshua A Welsh, Kenneth W. Witwer, Rienk Nieuwland
Blood is the most commonly used body fluid for extracellular vesicle (EV) research. The composition of a blood sample and its derivatives (i.e., plasma and serum) are not only donor-dependent but also influenced by collection and preparation protocols. Since there are hundreds of pre-analytical protocols and over forty variables, the development of standard operating procedures for EV research is very
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Detection of the interactions of tumour derived extracellular vesicles with immune cells is dependent on EV-labelling methods J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-11-30 Luisa Loconte, Davinia Arguedas, Rojbin El, Alix Zhou, Anna Chipont, Lea Guyonnet, Coralie Guerin, Ester Piovesana, José Luis Vázquez-Ibar, Alain Joliot, Clotilde Théry, Lorena Martín-Jaular
Cell-cell communication within the complex tumour microenvironment is critical to cancer progression. Tumor-derived extracellular vesicles (TD-EVs) are key players in this process. They can interact with immune cells and modulate their activity, either suppressing or activating the immune system. Deciphering the interactions between TD-EVs and immune cells is essential to understand immune modulation
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Nanoinjection of extracellular vesicles to single live cells by robotic fluidic force microscopy J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-11-30 Kinga Dóra Kovács, Tamás Visnovitz, Tamás Gerecsei, Beatrix Peter, Sándor Kurunczi, Anna Koncz, Krisztina Németh, Dorina Lenzinger, Krisztina V. Vukman, Anna Balogh, Imola Rajmon, Péter Lőrincz, Inna Székács, Edit I. Buzás, Robert Horvath
In the past decade, extracellular vesicles (EVs) have attracted substantial interest in biomedicine. With progress in the field, we have an increasing understanding of cellular responses to EVs. In this Technical Report, we describe the direct nanoinjection of EVs into the cytoplasm of single cells of different cell lines. By using robotic fluidic force microscopy (robotic FluidFM), nanoinjection of
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Proteome and immune responses of extracellular vesicles derived from macrophages infected with the periodontal pathogen Tannerella forsythia J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-11-28 Younggap Lim, Hyun Young Kim, Dohyun Han, Bong-Kyu Choi
Periodontitis is a chronic inflammatory disease caused by periodontal pathogens in subgingival plaque and is associated with systemic inflammatory diseases. Extracellular vesicles (EVs) released from host cells and pathogens carry a variety of biological molecules and are of interest for their role in disease progression and as diagnostic markers. In the present study, we analysed the proteome and
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Three-dimensional reconstruction of interstitial extracellular vesicles in human liver as determined by electron tomography J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-11-27 Roger Olofsson Bagge, Jens Berndtsson, Ornella Urzì, Jan Lötvall, Massimo Micaroni, Rossella Crescitelli
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Circulating extracellular vesicles in healthy and pathological pregnancies: A scoping review of methodology, rigour and results J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-11-16 Megan V. C. Barnes, Paschalia Pantazi, Beth Holder
Extracellular vesicles (EVs) play a crucial role in pregnancy, revealed by the presence of placental-derived EVs in maternal blood, their in vitro functionality, and their altered cargo in pregnancy pathologies. These EVs are thought to be involved in the development of pregnancy pathologies, such as pre-eclampsia, pre-term birth, and fetal growth restriction, and have been suggested as a source of
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Engineering high-affinity dual targeting cellular nanovesicles for optimised cancer immunotherapy J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-11-16 Luyao Zhang, Xu Zhao, Yanan Niu, Xiaoya Ma, Wei Yuan, Jie Ma
Dual targeting to immune checkpoints has achieved a better therapeutic efficacy than single targeting due to synergistic extrication of tumour immunity. However, most dual targeting strategies are usually antibody dependent which facing drawbacks of antibodies, such as poor solid tumour penetration and unsatisfied affinity. To meet the challenges, we engineered a cell membrane displaying a fusion protein
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Physical association of low density lipoprotein particles and extracellular vesicles unveiled by single particle analysis J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-11-09 Estefanía Lozano-Andrés, Agustin Enciso-Martinez, Abril Gijsbers, Andrea Ridolfi, Guillaume Van Niel, Sten F. W. M. Libregts, Cláudio Pinheiro, Martijn J. C. van Herwijnen, An Hendrix, Marco Brucale, Francesco Valle, Peter J. Peters, Cees Otto, Ger J. A. Arkesteijn, Marca H. M. Wauben
Extracellular vesicles (EVs) in blood plasma are recognized as potential biomarkers for disease. Although blood plasma is easily obtainable, analysis of EVs at the single particle level is still challenging due to the biological complexity of this body fluid. Besides EVs, plasma contains different types of lipoproteins particles (LPPs), that outnumber EVs by orders of magnitude and which partially
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RTP801 mediates transneuronal toxicity in culture via extracellular vesicles J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-11-06 Júlia Solana-Balaguer, Núria Martín-Flores, Pol Garcia-Segura, Genís Campoy-Campos, Leticia Pérez-Sisqués, Almudena Chicote-González, Joaquín Fernández-Irigoyen, Enrique Santamaría, Esther Pérez-Navarro, Jordi Alberch, Cristina Malagelada
Extracellular vesicles (EVs) play a crucial role in intercellular communication, participating in the paracrine trophic support or in the propagation of toxic molecules, including proteins. RTP801 is a stress-regulated protein, whose levels are elevated during neurodegeneration and induce neuron death. However, whether RTP801 toxicity is transferred trans-neuronally via EVs remains unknown. Hence,
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Engineering a tunable micropattern-array assay to sort single extracellular vesicles and particles to detect RNA and protein in situ J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-10-31 Jingjing Zhang, Xilal Y. Rima, Xinyu Wang, Luong T. H. Nguyen, Kristin Huntoon, Yifan Ma, Paola Loreto Palacio, Kim Truc Nguyen, Karunya Albert, Minh-Dao Duong-Thi, Nicole Walters, Kwang Joo Kwak, Min Jin Yoon, Hong Li, Jacob Doon-Ralls, Colin L. Hisey, Daeyong Lee, Yifan Wang, Jonghoon Ha, Kelsey Scherler, Shannon Fallen, Inyoul Lee, Andre F. Palmer, Wen Jiang, Setty M. Magaña, Kai Wang, Betty Y.
The molecular heterogeneity of extracellular vesicles (EVs) and the co-isolation of physically similar particles, such as lipoproteins (LPs), confounds and limits the sensitivity of EV bulk biomarker characterization. Herein, we present a single-EV and particle (siEVP) protein and RNA assay (siEVPPRA) to simultaneously detect mRNAs, miRNAs, and proteins in subpopulations of EVs and LPs. The siEVPPRA
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Palmitate and glucose increase amyloid precursor protein in extracellular vesicles: Missing link between metabolic syndrome and Alzheimer's disease J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-10-28 Bhumsoo Kim, Yoon-Tae Kang, Faye E. Mendelson, John M. Hayes, Masha G. Savelieff, Sunitha Nagrath, Eva L. Feldman
The metabolic syndrome (MetS) and Alzheimer's disease share several pathological features, including insulin resistance, abnormal protein processing, mitochondrial dysfunction and elevated inflammation and oxidative stress. The MetS constitutes elevated fasting glucose, obesity, dyslipidaemia and hypertension and increases the risk of developing Alzheimer's disease, but the precise mechanism remains
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Culture conditions greatly impact the levels of vesicular and extravesicular Ago2 and RNA in extracellular vesicle preparations J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-10-26 Lizandra Jimenez, Bahnisikha Barman, Youn Jae Jung, Lauren Cocozza, Evan Krystofiak, Cherie Saffold, Kasey C. Vickers, John T. Wilson, T. Renee Dawson, Alissa M. Weaver
Extracellular vesicle (EV)-carried miRNAs can influence gene expression and functional phenotypes in recipient cells. Argonaute 2 (Ago2) is a key miRNA-binding protein that has been identified in EVs and could influence RNA silencing. However, Ago2 is in a non-vesicular form in serum and can be an EV contaminant. In addition, RNA-binding proteins (RBPs), including Ago2, and RNAs are often minor EV
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Correction to “ApoE expression in macrophages communicates immunometabolic signaling that controls hyperlipidemia-driven hematopoiesis & inflammation via extracellular vesicles” J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-10-26
Phu, T. A., Ng, M., Vu, N. K., Gao, A. S., & Raffai, R. L. (2023). ApoE expression in macrophages communicates immunometabolic signaling that controls hyperlipidemia-driven hematopoiesis & inflammation via extracellular vesicles. Journal of Extracellular Vesicles, 12, e12345. https://doi.org/10.1002/jev2.12345 In the originally published article, the incorrect GSE accession number was reported in Section
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Plant exosomes fused with engineered mesenchymal stem cell-derived nanovesicles for synergistic therapy of autoimmune skin disorders J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-10-19 Rufan Huang, Bo Jia, Dandan Su, Manchun Li, Zhanxue Xu, Chao He, Yisheng Huang, Hang Fan, Hongbo Chen, Fang Cheng
Existing therapeutics for autoimmune diseases remain problematic due to low efficacy, severe side effects, and difficulties to reach target tissues. Herein, we design multifunctional fusion nanovesicles that can target lesions for the treatment of autoimmune skin diseases. The grapefruit-derived exosome-like nanovesicles (GEVs) with anti-inflammatory and antioxidant effects are first encapsulated with
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Correction to T-cell derived extracellular vesicles prime macrophages for improved STING based cancer immunotherapy J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-10-19
Aida S. Hansen, Lea S. Jensen, Kristine R. Gammelgaard, Kristoffer G. Ryttersgaard, Christian Krapp, Jesper Just, Kasper L. Jønsson, Pia B. Jensen, Thomas Boesen, Mogens Johannsen, Anders Etzerodt, Bent W. Deleuran, Martin R. Jakobse In the original article, author Mogens Johannsen's name was misspelled as Mogens Johansen. This has been corrected in the online version of the article. We apologize for
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Correction to "A quick pipeline for the isolation of 3D cell culture-derived extracellular vesicles" J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-10-18
Kyykallio, H., Faria, A. V. S., Hartman, R., Capra, J., Rilla, K., & Siljander, P. R.-M. (2022). A quick pipeline for the isolation of 3D cell culture-derived extracellular vesicles. Journal of Extracellular Vesicles, 11, e12273. https://doi.org/10.1002/jev2.12273 In the originally published article, author Rosabella Hartman's last name and email address was spelled incorrectly. This has been corrected
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Particle profiling of EV-lipoprotein mixtures by AFM nanomechanical imaging J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-10-19 Andrea Ridolfi, Laura Conti, Marco Brucale, Roberto Frigerio, Jacopo Cardellini, Angelo Musicò, Miriam Romano, Andrea Zendrini, Laura Polito, Greta Bergamaschi, Alessandro Gori, Costanza Montis, Stefano Panella, Lucio Barile, Debora Berti, Annalisa Radeghieri, Paolo Bergese, Marina Cretich, Francesco Valle
The widely overlapping physicochemical properties of lipoproteins (LPs) and extracellular vesicles (EVs) represents one of the main obstacles for the isolation and characterization of these pervasive biogenic lipid nanoparticles. We herein present the application of an atomic force microscopy (AFM)-based quantitative morphometry assay to the rapid nanomechanical screening of mixed LPs and EVs samples
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Extracellular vesicles from retinal pigment epithelial cells expressing R345W-Fibulin-3 induce epithelial-mesenchymal transition in recipient cells J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-10-19 Mi Zhou, Yuanjun Zhao, Sarah R. Weber, Christopher Gates, Nicholas J. Carruthers, Han Chen, Xiaoming Liu, Hong-Gang Wang, Michael Ford, Matthew T. Swulius, Alistair J. Barber, Stephanie L. Grillo, Jeffrey M. Sundstrom
We have shown previously that expression of R345W-Fibulin-3 induces epithelial-mesenchymal transition (EMT) in retinal pigment epithelial (RPE) cells. The purpose of the current study was to determine if extracellular vesicles (EVs) derived from RPE cells expressing R345W-Fibulin-3 mutation are sufficient to induce EMT in recipient cells. ARPE-19 cells were infected with luciferase-tagged wild-type
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Systematic compositional analysis of exosomal extracellular vesicles produced by cells undergoing apoptosis, necroptosis and ferroptosis J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-10-08 Benjamin Cappe, Mike Vadi, Eliza Sack, Ludivine Wacheul, Bruno Verstraeten, Sara Dufour, Julien Franck, Wei Xie, Francis Impens, An Hendrix, Denis L. J. Lafontaine, Peter Vandenabeele, Franck B. Riquet
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Dr. Eberhard Trams—The man who coined the name “exosomes”—A prescient but largely forgotten pioneer J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-10-04 Ewen MacDonald, Norman Salem Jr
While the approved term to describe the membrane-bound particles released from cells is now “extracellular vesicles,” there is no doubt that the trivial term “exosomes” is now firmly embedded into the scientific lexicon. Recently, a history of extracellular vesicle research was published (Couch et al., 2021). In it, a passing mention is made to the group working under Dr. Eberhard Trams in the Laboratory
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Engineered small extracellular vesicle‑mediated NOX4 siRNA delivery for targeted therapy of cardiac hypertrophy J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-10-05 Ji-Young Kang, Dasom Mun, Yumin Chun, Da-Seul Park, Hyoeun Kim, Nuri Yun, Boyoung Joung
Small-interfering RNA (siRNA) therapy is considered a powerful therapeutic strategy for treating cardiac hypertrophy, an important risk factor for subsequent cardiac morbidity and mortality. However, the lack of safe and efficient in vivo delivery of siRNAs is a major challenge for broadening its clinical applications. Small extracellular vesicles (sEVs) are a promising delivery system for siRNAs but
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Inhibition of extracellular vesicle-derived miR-146a-5p decreases progression of melanoma brain metastasis via Notch pathway dysregulation in astrocytes J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-09-27 Emma Rigg, Jiwei Wang, Zhiwei Xue, Taral R. Lunavat, Guowei Liu, Tuyen Hoang, Himalaya Parajuli, Mingzhi Han, Rolf Bjerkvig, Petr V. Nazarov, Nathalie Nicot, Stephanie Kreis, Christiane Margue, Miléne Tetsi Nomigni, Jochen Utikal, Hrvoje Miletic, Terje Sundstrøm, Lars A. R. Ystaas, Xingang Li, Frits Thorsen
Melanoma has the highest propensity of all cancers to metastasize to the brain with a large percentage of late-stage patients developing metastases in the central nervous system (CNS). It is well known that metastasis establishment, cell survival, and progression are affected by tumour-host cell interactions where changes in the host cellular compartments likely play an important role. In this context
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Neuron-derived extracellular vesicles contain synaptic proteins, promote spine formation, activate TrkB-mediated signalling and preserve neuronal complexity J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-09-24 Julia Solana-Balaguer, Genís Campoy-Campos, Núria Martín-Flores, Leticia Pérez-Sisqués, Laia Sitjà-Roqueta, Melike Kucukerden, Ana Gámez-Valero, Albert Coll-Manzano, Eulàlia Martí, Esther Pérez-Navarro, Jordi Alberch, Jordi Soriano, Mercè Masana, Cristina Malagelada
Extracellular vesicles (EVs) play an important role in intercellular communication as carriers of signalling molecules such as bioactive miRNAs, proteins and lipids. EVs are key players in the functioning of the central nervous system (CNS) by influencing synaptic events and modulating recipient neurons. However, the specific role of neuron-to-neuron communication via EVs is still not well understood
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Engineering extracellular vesicles to deliver CRISPR ribonucleoprotein for gene editing J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-09-18 Joseph Andrew Whitley, Houjian Cai
Clustered regularly interspaced palindromic repeats (CRISPR) is a gene editing tool with tremendous therapeutic potential. Recently, ribonucleoprotein (RNP) complex-based CRISPR systems have gained momentum due to their reduction of off-target editing. This has coincided with the emergence of extracellular vesicles (EVs) as a therapeutic delivery vehicle due to its low immunogenicity and high capacity
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Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion J. Extracell. Vesicles (IF 16.0) Pub Date : 2023-09-12 Chuqi Liu, Dexiang Liu, Shen Wang, Lu Gan, Xiangliang Yang, Cong Ma
Exosomes play crucial roles in local and distant cellular communication and are involved in various physiological and pathological processes. Tumour-derived exosomes are pivotal to tumorigenesis, but the precise mechanisms underlying their secretion remain elusive. In particular, the SNARE proteins that mediate the fusion of multivesicular bodies (MVBs) with the plasma membrane (PM) in tumour cells