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Usefulness of sulfonated azo dyes to evaluate macromolecularly oriented protein substrates Acta Histochem. (IF 2.5) Pub Date : 2024-03-12 Maria Luiza S. Mello, Eli H.M. dos Anjos, Benedicto de Campos Vidal
Sulfonated azo dyes are crucial for the histochemical, topochemical, and electrophoretic demonstration of proteins. Additionally, these dyes may reveal the significance of evaluating the anisotropic phenomenon of linear dichroism in macromolecularly oriented stained proteins. However, this requires that the ordered –NH groups available for electrostatic binding of the -SO dye groups are present in
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Investigation of the differentiation potential of pericyte cells as an alternative source of mesenchymal stem cells Acta Histochem. (IF 2.5) Pub Date : 2024-03-02 Selen Polat, Yusufhan Yazir, Gökhan Duruksu, Kamil Can Kiliç, Serap Mert, Gülçin Gacar, Büşra Öncel Duman, Zehra Seda Halbutoğullari
The mesenchymal stem cells (MSCs) with characterized by their multipotency and capacity to differentiate into various tissue cell types, have led to their incorporation in regenerative medicine research. However, the limited numbers of MSCs in the human body and their diverse differentiation capabilities in tissues highlight the need for exploring alternative regenerative cell sources. In this study
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Cancer-induced morphological changes in enteric glial cells in the jejunum of Walker-256 tumor-bearing rats Acta Histochem. (IF 2.5) Pub Date : 2024-02-28 Fabiana Galvão da Motta Lima, Maysa Pacheco Alvarez da Silva, Sabrina Silva Sestak, Flávia Alessandra Guarnier, Ana Paula de Oliveira, João Victor Kuller, Brian David Gulbransen, Juliana Vanessa Colombo Martins Perles, Jacqueline Nelisis Zanoni
Cancer-induced cachexia is associated with systemic inflammation and gastrointestinal dysfunction. How changes to cells of the enteric nervous system contribute to gut dysfunction in tumor development and cancer cachexia is unknown. Here, we tested the hypothesis that changes to enteric glia, a type of peripheral glia that surround enteric neurons and regulate gut homeostasis, are associated with tumor
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Immunolocalization patterns of histone-deacetylases in salivary glands of mice during postnatal development Acta Histochem. (IF 2.5) Pub Date : 2024-02-21 Chubo Yang, Xuejing Song, Jiaqi Kong, Huishu Li, Yuanbo Zhan
Histone-deacetylases (HDACs) are epigenetic modulators involved in the control of gene expression. No data are available on the expression or subcellular localization of HDACs in salivary glands. The present study aims to examine the subcellular distribution of HDACs in salivary glands during postnatal development. The major salivary glands of C57/BL6 mice were separately removed at 10, 25, 30,60 and
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Sialylation status and its relationship with morphofunctional changes in human adult testis during sexually mature life and aging: A narrative review Acta Histochem. (IF 2.5) Pub Date : 2024-02-20 Mirko Manetti, Mirca Marini, Angelica Perna, Alessia Tani, Eleonora Sgambati
Sialic acids (Sias) are a family of electronegatively charged nine-carbon monosaccharides containing a carboxylic acid, mostly found as terminal residues in glycans of glycoproteins and glycolipids. They are bound to galactose or N-acetylgalactosamine via α2,3 or α2,6 linkage, or to other Sias especially via α2,8 linkage, which results in monomeric, oligomeric, and polymeric forms. Sias play determinant
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Delineation of the healthy rabbit duodenum by immunohistochemistry – A short communication Acta Histochem. (IF 2.5) Pub Date : 2024-01-31 Gabriella Meier Bürgisser, Dorothea M. Heuberger, Pietro Giovanoli, Maurizio Calcagni, Johanna Buschmann
The duodenum acts as a vital organ that performs fundamental physiological functions like digestion and nutrient absorption. Situated in the lower abdomen, the duodenum is located between the stomach and the jejunum. Usually, the duodenum is divided into four anatomical portions. We here compare paraffin embedded and cryosections of the healthy rabbit duodenum for research purposes. This analysis evaluates
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ECM1-associated miR-1260b promotes osteogenic differentiation by targeting GDI1 Acta Histochem. (IF 2.5) Pub Date : 2024-01-23 Jiangxia Li, Ke Xu, Yunqing Cui, Tianyuan Xu, Wenchao Fei, Cuiting Lyu, Yinjue Yu, Lina Yang, Yang Hong, Gong Yang
Osteoporosis (OP) is a common disease among older adults. The promotion of osteoblast differentiation plays a crucial role in alleviating OP symptoms. Extracellular matrix protein 1 (ECM1) has been reported to be closely associated with osteogenic differentiation. In this study, we constructed U2OS cell lines with ECM1 knockdown and ECM1a overexpression based on knockdown, and identified the target
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Protective effect of FXN overexpression on ferroptosis in L-Glu-induced SH-SY5Y cells Acta Histochem. (IF 2.5) Pub Date : 2024-01-23 Mengran Wang, Tingting Xuan, Haining Li, Jing An, Tianhui Hao, Jiang Cheng
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SIRT1 regulates endoplasmic reticulum stress-related organ damage Acta Histochem. (IF 2.5) Pub Date : 2024-01-17 He Hu, Weichao Xu, Yan Li, Zhicheng Wang, Siyue Wang, Yansheng Liu, Minan Bai, Yingying Lou, Qian Yang
Endoplasmic reticulum (ER) stress plays a key role in the pathogenesis of several organ damages. Studies show that excessive ER stress (ERS) can destroy cellular homeostasis, causing cell damage and physiological dysfunction in various organs. In recent years, Sirtuin1 (SIRT1) has become a research hotspot on ERS. Increasing evidence suggests that SIRT1 plays a positive role in various ERS-induced
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Antioncogenic roles of USP9Y and DDX3Y in lung cancer: USP9Y stabilizes DDX3Y by preventing its degradation through deubiquitination Acta Histochem. (IF 2.5) Pub Date : 2024-01-12 Lei Xiu, Bo Ma, Lili Ding
In previous studies, downregulation of USP9Y and DDX3Y in lung cancer (LC) tissues was identified, while their function in LC progression remains elusive. In our current work, we intended to elucidate the effect and mechanisms of USP9Y and DDX3Y in LC. Gene downregulation has been confirmed in our LC tissues and cells. The effect of USP9Y or DDX3Y on LC cell malignancies was analyzed by functional
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Corrigendum to “Peroxisomal trans-2-enoyl-CoA inhibits proliferation, migration and invasion of hepatocellular carcinoma cells” [Acta Histochem. 125 (2023) 152002] Acta Histochem. (IF 2.5) Pub Date : 2024-01-04 Qingqing Luo, Liewang Qiu, Ke Zhan, Lu Zeng, Shengtao Liao, Chuanfei Li, Zhechuan Mei, Lin Lv
Abstract not available
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Astrocyte S100β expression and selective differentiation to GFAP and GS in the entorhinal cortex during ageing in the 3xTg-Alzheimer's disease mouse model Acta Histochem. (IF 2.5) Pub Date : 2023-12-29 J.J. Rodríguez, E. Gardenal, F. Zallo, A. Arrue, Joan Cabot, X. Busquets
The study of astrocytes and its role in the development and evolution of neurodegenerative diseases, including Alzheimer’s disease (AD) is essential to fully understand their aetiology. The aim if this study is to deepen into the concept of the heterogeneity of astrocyte subpopulations in the EC and in particular the identification of differentially functioning astrocyte subpopulations that respond
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Aflatoxin B1 and viruses’ combined pathogenesis: A mini systematics review of invitro and invivo studies Acta Histochem. (IF 2.5) Pub Date : 2023-12-14 Mehdi Ahmadi, Ramin Shahbahrami, Fatemeh Khajeh, Sepideh Khodaeivandi, Ehsan Kakavandi, Reza Hazrati Raziabad, Kiandokht Ghanati
Introduction The combined pathogenesis of Aflatoxin B1 (AFB1) and several viruses such as HBV, EBV and influenza virus have been investigated yet the molecular mechanism of their interaction and possible synergistic effects is not fully understood. Objectives The aim of the current systematic review was to review in-vitro and in-vivo studies investigating the combined pathogenesis of aflatoxins and
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Synergies in stem cell research: Integrating technologies, strategies, and bionanomaterial innovations Acta Histochem. (IF 2.5) Pub Date : 2023-12-02 Sharda Bharti, Awanish Kumar
Since the 1960 s, there has been a substantial amount of research directed towards investigating the biology of several types of stem cells, including embryonic stem cells, brain cells, and mesenchymal stem cells. In contemporary times, a wide array of stem cells has been utilized to treat several disorders, including bone marrow transplantation. In recent years, stem cell treatment has developed as
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Epidermal and dermal cells from adult rat eccrine sweat gland-containing skin can reconstruct the three-dimensional structure of eccrine sweat glands Acta Histochem. (IF 2.5) Pub Date : 2023-12-01 Cangyu Wang, Manxiu Cao, Junhong Zhao, Anqi Hu, Xiang Liu, Zihua Chen, Cuiping Zhang, Haihong Li
Background Previously, we have demonstrated that eccrine sweat gland cells (ESGCs) can reconstruct the three-dimensional (3D) structure of eccrine sweat glands (ESGs). However, there is still a need to explore source cells capable of regenerating ESG to address the issue of ESG regeneration in ESGC-deficient conditions, such as severe burns. Methods The epidermal cells and dermal cells in adult rat
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Delineation of the healthy rabbit tongue by immunohistochemistry – A technical note Acta Histochem. (IF 2.5) Pub Date : 2023-11-30 Gabriella Meier Bürgisser, Dorothea M. Heuberger, Julia Rieber, Iris Miescher, Pietro Giovanoli, Maurizio Calcagni, Johanna Buschmann
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Catalpol antagonizes LPS-mediated inflammation and promotes osteoblast differentiation through the miR-124-3p/DNMT3b/TRAF6 axis Acta Histochem. (IF 2.5) Pub Date : 2023-11-30 Pan Zhang, Qun Feng, Wenxiao Chen, Xizhuang Bai
Background Dysregulated inflammation and osteoblast differentiation are implicated in osteoporosis. Exploring the activity of catalpol in inflammation and osteoblast differentiation deepens the understanding of osteoporosis pathogenesis. Methods LPS was used to treated hFOB1.19 cells to induce inflammation and repress osteoblast differentiation. FOB1.19 cells were induced in osteoblast differentiation
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BrdU does not induce hepatocellular damage in experimental Wistar rats Acta Histochem. (IF 2.5) Pub Date : 2023-11-27 Abril Alondra Barrientos-Bonilla, Paola Belem Pensado-Guevara, Abraham Puga-Olguín, Rasajna Nadella, Aurora del Carmen Sánchez-García, Laura Mireya Zavala-Flores, Arnulfo Villanueva-Olivo, Iliana Tamara Cibrián-Llanderal, María de Jesús Rovirosa-Hernández, Daniel Hernandez-Baltazar
Bromodeoxyuridine (BrdU) is used in studies related to cell proliferation and neurogenesis. The multiple intraperitoneal injections of this molecule could favor liver function profile changes. In this study, we evaluate the systemic and hepatocellular impact of BrdU in male adult Wistar rats in 30 %-partial hepatectomy (PHx) model. The rats received BrdU 50 mg/Kg by intraperitoneal injection at 0.5
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Association between neuropeptides and mucins in Crohn’s disease mucous cells Acta Histochem. (IF 2.5) Pub Date : 2023-11-17 Anthea Miller, Giuseppina Cutroneo, Giorgia Pia Lombardo, Roberta D’Angelo, Socrate Pallio, Alba Migliorato, Angelo Fumia, Angelo Favaloro, Eugenia Rita Lauriano, Simona Pergolizzi
Crohn’s disease (CD) and ulcerative colitis (UC) are both inflammatory bowel diseases (IBD). Unlike UC, which is limited to the mucosa of the colon, CD inflammation is characterized by chronic mucosal ulcerations affecting the entire gastrointestinal tract. Goblet cells (GCs) can be found in some lining epithelia, particularly in the respiratory and digestive tracts. GCs represent the main source of
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Histological and cytochemical analysis of the brain under conditions of hypobaric hypoxia-induced oxygen deficiency in albino rats Acta Histochem. (IF 2.5) Pub Date : 2023-11-17 Ruzanna Shushanyan, Anna Grigoryan, Tamara Abgaryan, Anna Karapetyan
High altitude sickness is a life-threatening disease that occurs among acclimatized individuals working or living at a high altitude accompanied by hypobaric hypoxia exposure. The prolonged influence of hypobaric hypoxia on the brain may trigger neuronal damage and cell death due to an oxygen deficiency. The purpose of the current study was to investigate the histomorphological changes in the hippocampus
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Kisspeptin (Kp-10) inhibits in vitro osteogenic differentiation of multipotent mesenchymal stromal cells extracted from the bone marrow of adult rats Acta Histochem. (IF 2.5) Pub Date : 2023-11-08 Laís Bitencourt Guimarães, Daniel Portela Dias Machado, Beatriz Ferreira Carvalho Versiani Caldeira, Larissa Tiemi Matuzake Vieira, Gabriela Alves Santos, Fabiana Rocha Araújo, Leonardo Teotônio Machado, Dawidson Assis Gomes, Natália de Melo Ocarino, Rogéria Serakides, Amanda Maria Sena Reis
Kisspeptin (Kp-10) is a neuropeptide that binds to GPR54 receptors, exerting several functions mainly in the nervous and reproductive systems of the body. However, its effects and mechanisms of action on the skeletal system remain poorly understood. This study evaluated the effects of different concentrations of Kp-10 on in vitro osteogenic differentiation of multipotent mesenchymal stromal cells (MSCs)
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Immunolocalization of lix1 in the regenerating tail of lizard indicates that the protein is mainly present in the nervous tissue Acta Histochem. (IF 2.5) Pub Date : 2023-11-08 Lorenzo Alibardi
Purpose Lizard regeneration derives from the re-activation of a number of developmental genes after tail amputation. Among genes with the highest expression, as indicated from the transcriptome, is lix1 which functional role is not known. Method An antibody that cross-reacts with the lizard Podarcis muralis lix1 has been utilized to detect by immunofluorescence the sites of localization of the protein
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In vitro validation: GLY alleviates UV-induced corneal epithelial damage through the HMGB1-TLR/MyD88-NF-κB signaling pathway Acta Histochem. (IF 2.5) Pub Date : 2023-11-06 XinYi Chen, XiaoXiao Zheng, Ting Shen, Ting He, YangQi Zhao, Yi Dong
UV-induced corneal damage is a common ocular surface injury that usually leads to corneal lesions causing persistent inflammation. High mobility group box 1 (HMGB1) is identified as an inflammatory alarm in various tissue injuries. Here, this study first evaluates the repair effect of the HMGB1-selective inhibitor GLY in UV-induced corneal damage; Secondly, the inhibitory effect of GLY on UV-induced
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The effect on m6A methylation writer complex by the reduced MATR3 in pterygium Acta Histochem. (IF 2.5) Pub Date : 2023-10-31 Qianqian Guo, Shichao Han
Pterygium is a common eye surface disease with high recurrence and unclear pathogenesis. In current study, RNA sequencing was conducted in 6 pairs of human pterygium and conjunctival tissues, and Matr3 as a novel candidate gene was significantly reduced in pterygium compared to control tissues. Moreover, immunoprecipitation was performed to pull down MATR3, and WTAP specially interacting with MATR3
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Saikosaponin-d regulates angiogenesis in idiopathic pulmonary fibrosis through angiopoietin/Tie-2 pathway Acta Histochem. (IF 2.5) Pub Date : 2023-10-12 Yan Wu, Jun Zhang, Xintian Wang, Yuncong Xu, Jinxu Zheng
Objective Idiopathic pulmonary fibrosis (IPF) is considered as a chronic interstitial lung disease with underlying mechanism of IPF remaining unclear, while there are no definitive treatment options. In recent years, scientists have gradually paid attention to the influence of angiogenesis on IPF. Because IPF is a progressive with microvascular remodeling disorder, scientists have postulated that angiogenesis
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MiR-223–3p overexpressed adipose mesenchymal stem cell-derived exosomes promote wound healing via targeting MAPK10 Acta Histochem. (IF 2.5) Pub Date : 2023-10-12 Xiaojiao Liu, Shunqiao Jin, Jiao Liu, Xuezhu Xu
Background Adipose mesenchymal stem cell (AMSC)-derived exosomes are promising novel factors for wound repair and regeneration. This study aimed to explore the potential roles and underlying mechanisms of specific miRNA in wound healing using AMSC-derived exosomes as carriers. Methods The expression profiles of GSE197840 were downloaded to screen for differentially expressed miRNAs (DEmiRNAs), and
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Inhibitory effect of telocyte-induced M1 macrophages on endometriosis: Targeting angiogenesis and invasion Acta Histochem. (IF 2.5) Pub Date : 2023-10-07 Xiao-Jiao Wei, Yue-lin Huang, Tian-Quan Chen, Xiao-Jun Yang
Purpose Telocytes (TCs), a novel type of stromal cells found in tissues, induce macrophage differentiation into classically activated macrophages (M1) types and enhance their phagocytic function. The purpose of this study was to investigate the inhibitory effects of TC-induced M1 macrophages on endometriosis (EMs). Methods mouse uterine primary TCs and endometrial stromal cells (ESCs) were isolated
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Transforming growth factor-beta superfamily regulates mesenchymal stem cell osteogenic differentiation: A microRNA linking Acta Histochem. (IF 2.5) Pub Date : 2023-10-07 Juan F. Santibanez, Cesar Echeverria, Carola Millan, Felipe Simon
The ability to differentiate into cells of different lineages, such as bone cells, is the principal value of adult mesenchymal stem cells (MSCs), which can be used with the final aim of regenerating damaged tissue. Due to its potential use and importance in regenerative medicine and tissue engineering, several questions have been raised regarding the molecular mechanisms of MSC differentiation. As
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PGC1α deficiency reverses cholestasis-induced liver injury via attenuating hepatic inflammation and promoting bile duct remodeling Acta Histochem. (IF 2.5) Pub Date : 2023-10-07 Dingwu Li, Chenhui Ye, Peihao Liu, Ting Sun, Yunsheng Qin, Xingyong Wan
Objectives Cholestatic liver diseases are characterized by hepatocellular damage, cholangiocyte proliferation, and progressive fibrosis. Bile duct ligation (BDL) is widely used to resemble liver injuries induced by cholestasis. Peroxisome proliferator-activated receptor-gamma coactivator 1 alpha (PGC1α) was reported to play a critical role in multiple biological responses. Nevertheless, whether PGC1α
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Delineation of the healthy rabbit tonsil by immunohistochemistry – A short communication Acta Histochem. (IF 2.5) Pub Date : 2023-10-05 Gabriella Meier Bürgisser, Dorothea M. Heuberger, Pietro Giovanoli, Maurizio Calcagni, Johanna Buschmann
Situated in the oral cavity, the rabbit palatine tonsils are part of the mucosal immune system and help to defend the body against foreign pathogens. Expressed as two oval protrusions in the wall of the oropharynx, the rabbit palatine tonsils are characterized by excretory ducts and trabeculae. We here compare paraffin embedded and cryosections of the healthy rabbit tonsils. This analysis centers on
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Immunohistochemical and ultrastructural identification of telocytes in the lamina propria of human vaginal mucosa Acta Histochem. (IF 2.5) Pub Date : 2023-09-25 Irene Rosa, Patrizia Nardini, Bianca Saveria Fioretto, Daniele Guasti, Eloisa Romano, Eleonora Sgambati, Mirca Marini, Mirko Manetti
Since their relatively recent discovery, telocytes (TCs) have been described as peculiar cells strategically positioned in the stromal tissue component of multiple organ systems of the mammalian body including female reproductive organs (i.e., ovary, uterine tube, and uterus). Nevertheless, current knowledge of TCs in the vagina is very limited. The present study was therefore undertaken to investigate
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Topiramate promotes osteogenic differentiation through AMPK-dependent phosphorylation of Smad1/5/9 Acta Histochem. (IF 2.5) Pub Date : 2023-09-25 Kyeong-Min Kim, Hyo-Eun Son, Young-Ju Lim, Won-Gu Jang
Topiramate [2,3:4,5-bis-o-(1-methylethylidene) β-D-fructo-pyranose sulfamate; TPM] is one of the most used new-generation antiepileptic drugs. It has been reported to regulate the differentiation of human bone cells. However, the molecular mechanism of TPM in osteoblast differentiation is not fully elucidated. In the present study, we examined the effect of TPM on osteogenic differentiation of C3H10T1/2
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Epithelial polarity-driven membrane separation but not cavitation regulates lumen formation of rat eccrine sweat glands Acta Histochem. (IF 2.5) Pub Date : 2023-09-25 Zixiu Chen, Junhong Zhao, Cangyu Wang, Xiang Liu, Zihua Chen, Jianda Zhou, Lei Zhang, Cuiping Zhang, Haihong Li
Background Each eccrine sweat gland (ESG) is a single-tubular structure with a central lumen, and the formation of hollow lumen in the initial solid cell mass is a key developmental process. To date, there are no reports on the mechanism of native ESG lumen formation. Methods To investigate the lumen morphogenesis and the lumen formation mechanisms of Sprague-Dawley (SD) rat ESGs, SD rat hind-footpads
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Tissue and subcellular localization of CycD2 and KRPs are dissimilarly distributed by glucose and sucrose during early maize germination Acta Histochem. (IF 2.5) Pub Date : 2023-09-15 Diana I. Romero-Sánchez, Sonia Vázquez-Santana, Rafael A. Alonso-Alvarez, Jorge M. Vázquez-Ramos, Aurora Lara-Núñez
In maize, immunoprecipitation assays have shown that CycD2;2 interacts with KRPs. However, evidence on CycD2;2 or KRPs localization and their possible interaction in specific tissues is lacking and its physiological consequence is still unknown. This work explores the spatiotemporal presence of CyclinD2s and KRPs, cell cycle regulators, during maize seed germination (18 and 36 h) after soaking on glucose
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Morphological analysis of cell cannibalism: An auxiliary tool in the prediction of central giant cell granuloma clinical behavior Acta Histochem. (IF 2.5) Pub Date : 2023-08-30 Caio César da Silva Barros, Luiz Miguel da Rocha Santos, Mara Luana Batista Severo, Márcia Cristina da Costa Miguel, Cristiane Helena Squarize, Éricka Janine Dantas da Silveira
Central giant cell granuloma (CGCG) is a benign jaw lesion with variable clinical behavior. Cell cannibalism is a cellular process associated with aggressiveness and invasion in malignant neoplasms. Here, we morphologically investigated cell cannibalism as an auxiliary method to predict CGCG clinical behavior. Cell cannibalism was quantitatively evaluated in 19 cases of peripheral giant cell granuloma
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A newly improved method of primary cell culture: Tissue block with continuous adhesion subculture in skin fibroblast Acta Histochem. (IF 2.5) Pub Date : 2023-08-26 Qiyan Deng, Lumei Liu, Ran Tang, Dehai Xian, Jianqiao Zhong
Background Fibroblasts (FBs) have been widely used as a typical in vitro cell model for investigating the biological processes and cell pathophysiological mechanisms. However, FBs are prone to senescence in cell culture process after several passages. Thus, a new approach to cell culture is quite required to enhance the viability of cells. Objective To explore a novel method of cell culture based on
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Corrigendum to “Intracerebral injection of oil cyst content of human craniopharyngioma (oil machinery fluid) as a toxic model in the rat brain” [Acta Histochem. 116(3) (2014) 448–56] Acta Histochem. (IF 2.5) Pub Date : 2023-08-11 Martha Lilia Tena-Suck, Ma. Elena Hernández-Campos, Alma Ortiz-Plata, Citlaltepetl Salinas-Lara, Ana Laura Colín-González, Abel Santamaría
Abstract not available
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Expression of type 1 vomeronasal receptors in the olfactory organ of the African lungfish, Protopterus dolloi Acta Histochem. (IF 2.5) Pub Date : 2023-08-02 Shoko Nakamuta, Atsuhiro Sakuma, Masato Nikaido, Hideaki Kato, Masao Miyazaki, Yoshio Yamamoto, Nobuaki Nakamuta
The vomeronasal organ is an olfactory organ found in amphibians and higher vertebrates. Type 1 vomeronasal receptors, one of the major olfactory receptors in vertebrates, are expressed in the vomeronasal organ in mammals. In amphibians and fish, they are expressed in the olfactory epithelium. The lungfish, which is the species of fish most closely related to amphibians, has a primitive vomeronasal
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SARS-CoV-2 induced changes in the glycosylation pattern in the respiratory tract of Golden Syrian hamsters Acta Histochem. (IF 2.5) Pub Date : 2023-07-29 Lea-Adriana Barlang, Björn-Patrick Mohl, Claudia Blaurock, Sophia Harder, Angele Breithaupt, Olivia M. Merkel, Anne Balkema-Buschmann, Andreas Popp
Even after more than two years of intensive research, not all of the pathophysiological processes of Coronavirus Disease 2019 (COVID-19), induced by severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) infection, have been fully elucidated. The initial virus-host interaction at the respiratory epithelium plays a crucial role in the course and progression of the infection, and is highly
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Regulation of Nur77-TLR4/MyD88 signaling pathway is required for Ginsenoside Rc ameliorates hepatic fibrosis regression by deactivating hepatic stellate cells Acta Histochem. (IF 2.5) Pub Date : 2023-07-30 Bo-Feng Qin, Shan Gao, Qi-yuan Feng, Wei Chen, Hai-Ming Sun, Jian Song
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Three-dimensional human germinal centers of different sizes in patients diagnosed with lymphadenitis show comparative constant relative volumes of B cells, T cells, follicular dendritic cells, and macrophages Acta Histochem. (IF 2.5) Pub Date : 2023-07-15
Germinal centers (GCs) are some of the most important structures in the human immune system. As such, their cell types and functions have been thoroughly investigated. B cells, T cells, follicular dendritic cells (FDCs), and macrophages have widely been found to typically be aggregated in GCs. However, the amount of space occupied by each of these cell types has yet to be investigated. In this study
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Corrigendum to “Hypoxia activates the PI3K/AKT/HIF-1α pathway to promote the anti-inflammatory effect of adipose mesenchymal stem cells” [Acta Histochem.125(4) (2023) 152042] Acta Histochem. (IF 2.5) Pub Date : 2023-07-13
Abstract not available
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The CellProfiler pipeline analysis of cell migration Acta Histochem. (IF 2.5) Pub Date : 2023-07-10 Nur Syamimi Ariffin
We demonstrate herein a refined method to evaluate the migration capacity of monolayer cells using the CellProfiler pipeline. We used MDA-MB-231 cells, a triple-negative breast cancer cell line, as a model to perform the wound healing assay and proceeded with the pipeline analysis. In order to see a contrast in our analysis of cell migration, we treated the cells with 10 µM kartogenin for 48 h and
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LSD1 knockdown confers protection against osteoclast formation by reducing histone 3 lysine 9 monomethylation and dimethylation in ITGB3 promoter Acta Histochem. (IF 2.5) Pub Date : 2023-07-07 Dongping Yu, Zhen Li, Jie Cao, Guowen Wei, Feng Shen
ITGB3, an osteoclast marker, is involved in osteoclast formation. Nevertheless, its related mechanism remains poorly characterized. Herein, this study examines the mechanisms affecting osteoclast formation with the involvement of ITGB3. Osteoclast formation was induced with macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear factor-kappa B ligand (RANKL), followed by measurement
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MCM5 is an oncogene of colon adenocarcinoma and promotes progression through cell cycle control Acta Histochem. (IF 2.5) Pub Date : 2023-06-27 Jiayan Mao, Jian Shen, Xuemei Lu, Ying Cai, Rujia Tao, Yuqin Deng, Yuanting Zhang, Yuan Wu, Wei Chen
Many patients with colon adenocarcinoma (COAD) are diagnosed at an advanced stage, and the molecular mechanism of COAD progression is intricate and controversial. Therefore, there is an urgent need to identify more novel prognosis biomarkers for COAD and elucidate the molecular mechanism of this disease. The present study aimed to screen out key genes correlated with COAD prognosis. In this study,
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The role of vascular endothelial cells in tumor metastasis Acta Histochem. (IF 2.5) Pub Date : 2023-06-20 Ying Feng, Shan Luo, Dandan Fan, Xingrong Guo, Shinan Ma
Vascular endothelial cells (VECs) are an integral component of the inner lining of blood vessels, and their functions are essential for the proper functioning of the vascular system. The tight junctions formed by VECs act as a significant barrier to the intravasation and extravasation of tumor cells (TCs). In addition to that, the proliferation, activation, and migration of VECs play a vital role in
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Study of platelet-rich fibrin promoting endothelial cell differentiation and angiogenesis induced by transplantation of adipose-derived stem cells Acta Histochem. (IF 2.5) Pub Date : 2023-06-15 Zhibing Ma, Jin Ding, Yawen Wang, Tianqi Zhang, Gang Chen, Jinlong Huang
Diabetic patients are characterized by long wound healing time, and adipose stem cells (ADSCs) can secrete growth factors to promote angiogenesis and improve diabetic wound healing. In this research, we attempted to interrogate the impact of platelet-rich fibrin (PRF) on ADSCs in diabetic wound healing. ADSCs were harvested from human adipose tissues and identified through flow cytometry. After pretreatment
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Astaxanthin inhibits oxidative stress and apoptosis in diabetic retinopathy Acta Histochem. (IF 2.5) Pub Date : 2023-06-19 Jian Fang, Wuxia Bai, Lina Yang
Background The pathophysiology of diabetic retinopathy (DR) is thought to be influenced by oxidative stress. Astaxanthin (ASX) is a natural product with antioxidant effect, but it is not clear whether its mechanism of inhibiting the development of DR is related to anti-oxidation. Methods Rats were intraperitoneally injected with streptozotocin (60 mg/kg) to create DR rat models followed by ASX (20 mg/kg)
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Phospholysine phosphohistidine inorganic pyrophosphate phosphatase suppresses human esophageal cancer cell growth by inducing mitotic catastrophe through the P27/cyclin A/CDK2 signaling pathway Acta Histochem. (IF 2.5) Pub Date : 2023-06-20 Duan Ruifang, Yang Changqing, Ren Chenxia, Li Ji, Wei Zibai
Esophageal cancer (ESCA) is a global dead malignancy with poor prognosis. However, its underlying molecular mechanism remains to be elucidated. Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP) has been reported as a tumor suppressor in multisystem cancer but its function in ESCA has not been reported. We analyzed LHPP expression between normal and tumor tissues of ESCA patients
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PRSS2 regulates EMT and metastasis via MMP-9 in gastric cancer Acta Histochem. (IF 2.5) Pub Date : 2023-06-16 Fei Wang, Jianfeng Yi, Yu Chen, Xiang Bai, Chunfeng Lu, Shichun Feng, Xiaojun Zhou
Serine protease 2 (PRSS2) is upregulated in gastric cancer tissues, correlates with poor prognosis and promotes migration and invasion of gastric cancer cells. However, the exact mechanism by which PRSS2 promotes metastasis in gastric cancer is unclear. We examined serum PRSS2 levels in healthy controls and gastric cancer patients by enzyme linked immunosorbent assay (ELISA) and analyzed the correlation
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Resveratrol as an antitumor agent for glioblastoma multiforme: Targeting resistance and promoting apoptotic cell deaths Acta Histochem. (IF 2.5) Pub Date : 2023-06-17 Sepideh Karkon-Shayan, Hasan Aliashrafzadeh, Hassan Dianat-Moghadam, Nima Rastegar-Pouyani, Mohammadreza Majidi, Mahdi Zarei, Sadegh moradi-vastegani, Yaser Bahramvand, Sepideh Babaniamansour, Emad Jafarzadeh
Glioblastoma multiforme (GBM) is one of the most aggressive brain and spinal cord tumors. Despite the significant development in application of antitumor drugs, no significant increases have been observed in the survival rates of patients with GBM, as GBM cells acquire resistance to conventional anticancer therapeutic agents. Multiple studies have revealed that PI3K/Akt, MAPK, Nanog, STAT 3, and Wnt
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Impacts of iron on ultrastructural features of NCI-H295R cell line related to steroidogenesis Acta Histochem. (IF 2.5) Pub Date : 2023-06-13 Hana Duranova, Veronika Fialkova, Veronika Simora, Jana Bilcikova, Peter Massanyi, Norbert Lukac, Zuzana Knazicka
The current study was intended to evaluate impacts of both iron (Fe) enrichment and overload (in the form of ferrous sulphate heptahydrate, FeSO4.7H2O) on ultrastructural characteristics of human adrenocarcinoma NCI-H295R cell line. Here, the NCI-H295R cells were treated with 0, 3.90, and 1000 µM FeSO40.7 H2O, and consequently proceeded for purposes of ultrastructural studies. Micrographs taken under
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Classification of colorectal cancer consensus molecular subtypes using attention-based multi-instance learning network on whole-slide images Acta Histochem. (IF 2.5) Pub Date : 2023-06-08 Huilin Xu, Aoshen Wu, He Ren, Chenghang Yu, Gang Liu, Lei Liu
Colorectal cancer (CRC) is the third most common and second most lethal cancer globally. It is highly heterogeneous with different clinical-pathological characteristics, prognostic status, and therapy responses. Thus, the precise diagnosis of CRC subtypes is of great significance for improving the prognosis and survival of CRC patients. Nowadays, the most commonly used molecular-level CRC classification
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Localization of alpha 2,6-linked sialic acid residues in gastrointestinal tract compartments of some tetrapod’s representatives: Comparative histochemical study Acta Histochem. (IF 2.5) Pub Date : 2023-05-30 Aziz Awaad, Ahmed Rushdy, Mohamed A. Adly
Epithelial mucins composed mainly of glycoproteins and play a vital role as protective barrier against a variety of harmful molecules and microbial infection. Additionally sialic acids, like glycoproteins, are considered as a main component of epithelial mucins and play an important role in mucosal immunity. For example, alpha 2,6-linked galactose/N-acetyl-galactosamine (Gal/GalNAc) sialic acid residues
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Immunohistochemical analysis of the vimentin filaments in Sertoli cells is a powerful tool for the prediction of spermatogenic dysfunction Acta Histochem. (IF 2.5) Pub Date : 2023-05-22 Hirokatsu Saito, Satoshi Yokota, Satoshi Kitajima
The close interaction between male germ cells and Sertoli cells, a type of somatic cell found in the seminiferous tubules of mammalian testis, is essential for the normal progression of spermatogenesis in mammals. Vimentin is an intermediate filament protein that primarily provides mechanical support, preserves cell shape, and maintains the nuclear position, and it is often used as a marker to identify
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Geniposide alleviates pressure overload in cardiac fibrosis with suppressed TGF-β1 pathway Acta Histochem. (IF 2.5) Pub Date : 2023-05-16 Yanmei Yao, Leqing Lin, Wenxue Tang, Yueliang Shen, Fayu Chen, Ning Li
Background Cardiac fibrosis is one of the main contributors to the pathogenesis of heart failure. Geniposide (GE), a major iridoid in gardenia fruit extract, has recently been reported to improve skeletal muscle fibrosis through the modulation of inflammation response. This investigation aimed to illuminate the cardio-protective effect and the potential mechanism of GE in cardiac fibrosis. Material
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KCCs, NKCCs, and NCC: Potential targets for cardiovascular therapeutics? A comprehensive review of cell and region specific expression and function Acta Histochem. (IF 2.5) Pub Date : 2023-05-16 Akshat D. Modi, Areej Naim Khan, Wing Yan Elizabeth Cheng, Dharmeshkumar M. Modi
Cardiovascular diseases, the leading life-threatening conditions, involve cardiac arrhythmia, coronary artery disease, myocardial infarction, heart failure, cardiomyopathy, and heart valve disease that are associated with the altered functioning of cation-chloride cotransporters. The decreased number of cation-chloride cotransporters leads to reduced reactivity to adrenergic stimulation. The KCC family
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Advantages of mesenchymal stem cell over the other stem cells Acta Histochem. (IF 2.5) Pub Date : 2023-05-09 Janani Gopalarethinam, Aswathy P. Nair, Mahalaxmi Iyer, Balachandar Vellingiri, Mohana Devi Subramaniam
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Hypoxia activates the PI3K/AKT/HIF-1α pathway to promote the anti-inflammatory effect of adipose mesenchymal stem cells Acta Histochem. (IF 2.5) Pub Date : 2023-05-01 Hongjing Ren, Mengchang Liu, Yueda Jihu, Huizhen Zeng, Chong Yao, Hong Yan
This study aimed to investigate the effect of hypoxia on the anti-inflammatory effect of adipose-derived mesenchymal stem cells (AMSCs) in vitro and its possible mechanism. AMSCs were cultured in vitro in a hypoxic environment with 3% O2, and a normoxic (21% O2) environment was used as the control. The cells were identified by in vitro adipogenic and osteogenic differentiation and cell surface antigen