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  • Membrane mucins of the intestine at a glance
    J. Cell Sci. (IF 4.517) Pub Date : 2020-03-13
    Pelaseyed, T., Hansson, G. C.

    Thaher Pelaseyed and Gunnar C. Hansson Membrane mucins cover most mucosal surfaces throughout the human body. The intestine harbors complex population of microorganisms (the microbiota) and numerous exogenous molecules that can harm the epithelium. In the colon, where the microbial burden is high, a mucus barrier forms the first line of defense by keeping bacteria away from the epithelial cells. In

    更新日期:2020-03-16
  • The lymph node at a glance - how spatial organization optimizes the immune response
    J. Cell Sci. (IF 4.517) Pub Date : 2020-03-06
    Grant, S. M., Lou, M., Yao, L., Germain, R. N., Radtke, A. J.

    Spencer M. Grant, Meng Lou, Li Yao, Ronald N. Germain, and Andrea J. Radtke A hallmark of the mammalian immune system is its ability to respond efficiently to foreign antigens without eliciting an inappropriate response to self-antigens. Furthermore, a robust immune response requires the coordination of a diverse range of cells present at low frequencies within the host. This problem is solved, in

    更新日期:2020-03-16
  • The plant-pathogen haustorial interface at a glance
    J. Cell Sci. (IF 4.517) Pub Date : 2020-03-04
    Bozkurt, T. O., Kamoun, S.

    Tolga O. Bozkurt and Sophien Kamoun Many filamentous pathogens invade plant cells through specialized hyphae called haustoria. These infection structures are enveloped by a newly synthesized plant-derived membrane called the extrahaustorial membrane (EHM). This specialized membrane is the ultimate interface between the plant and pathogen, and is key to the success or failure of infection. Strikingly

    更新日期:2020-03-16
  • Macrophages eat more after disruption of cis interactions between CD47 and the checkpoint receptor SIRPα.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-01-21
    Brandon H Hayes,Richard K Tsai,Lawrence J Dooling,Siddhant Kadu,Justine Y Lee,Diego Pantano,Pia L Rodriguez,Shyamsundar Subramanian,Jae-Won Shin,Dennis E Discher

    The macrophage checkpoint receptor SIRPα signals against phagocytosis by binding CD47 expressed on all cells - including macrophages. Here, inhibiting cis interactions between SIRPα and CD47 on the same macrophage increases eating approximately the same as inhibiting trans interactions. Antibody blockade of CD47, as pursued in clinical trials against cancer, is applied separately to human-derived macrophages

    更新日期:2020-03-16
  • Distinct actin cytoskeleton behaviour in primary and immortalised T-cells.
    J. Cell Sci. (IF 4.517) Pub Date : 2019-08-16
    Huw Colin-York,Sudha Kumari,Liliana Barbieri,Lena Cords,Marco Fritzsche

    Cytoskeletal actin dynamics are crucial for the activation of T-cells. Immortalised Jurkat T-cells have been the model system of choice to examine and correlate the dynamics of the actin cytoskeleton and the immunological synapse leading to T-cell activation. However, it has remained unclear whether immortalised cellular systems, such as Jurkat T-cells can recapitulate the cytoskeletal behaviour of

    更新日期:2020-03-16
  • DDX56 inhibits type I interferon by disrupting assembly of IRF3-IPO5 to inhibit IRF3 nucleus import.
    J. Cell Sci. (IF 4.517) Pub Date : 2019-07-24
    Dan Li,Shaozu Fu,Zhengqian Wu,Wenping Yang,Yi Ru,Hongbing Shu,Xiangtao Liu,Haixue Zheng

    Transcription factor IRF3-mediated type I interferon induction plays a role in antiviral innate immunity. However, mechanisms for the control and regulation of IRF3 nuclear import remain largely unknown. We have identified DEAD box polypeptide 56 (DDX56) as a negative regulator of virus-triggered IFN-β induction. Overexpression of DDX56 suppressed nuclear translocation of IRF3 via disrupting the IRF3-IOP5

    更新日期:2020-03-16
  • NKG2D-DAP10 signaling recruits EVL to the cytotoxic synapse to generate F-actin and promote NK cell cytotoxicity.
    J. Cell Sci. (IF 4.517) Pub Date : 2019-07-18
    Katelynn M Wilton,Brittany L Overlee,Daniel D Billadeau

    Natural killer (NK) cells eliminate abnormal cells through the release of cytolytic granule contents. In this process, NK cells must adhere to target cells through integrin-mediated adhesion, which is highly dependent on the generation of F-actin. Ena/VASP-like (EVL) is an actin regulatory protein previously shown to regulate integrin-mediated adhesion in other cell types, but its role in NK cell biology

    更新日期:2020-03-16
  • Adhesion, motility and matrix-degrading gene expression changes in CSF-1-induced mouse macrophage differentiation.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-01-31
    Michael W Murrey,James H Steer,Eloise L Greenland,Julie M Proudfoot,David A Joyce,Fiona J Pixley

    Migratory macrophages play critical roles in tissue development, homeostasis and disease, so it is important to understand how their migration machinery is regulated. Whole-transcriptome sequencing revealed that CSF-1-stimulated differentiation of bone marrow-derived precursors into mature macrophages is accompanied by widespread, profound changes in the expression of genes regulating adhesion, actin

    更新日期:2020-03-16
  • RPTPε promotes M2-polarized macrophage migration through ROCK2 signaling and podosome formation.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-01-21
    Fanny Lapointe,Sylvie Turcotte,Joanny Roy,Elyse Bissonnette,Marek Rola-Pleszczynski,Jana Stankova

    Cysteinyl-leukotrienes (cys-LTs) have well-characterized physiopathological roles in the development of inflammatory diseases. We have previously found that protein tyrosine phosphatase ε (PTPε) is a signaling partner of CysLT1R, a high affinity receptor for leukotriene D4 (LTD4). There are two major isoforms of PTPε, receptor-like (RPTPε) and cytoplasmic (cyt-)PTPε, both of which are encoded by the

    更新日期:2020-03-16
  • Novel role of proton-secreting epithelial cells in sperm maturation and mucosal immunity.
    J. Cell Sci. (IF 4.517) Pub Date : 2019-11-18
    Maria A Battistone,Raul German Spallanzani,Alexandra C Mendelsohn,Diane Capen,Anil V Nair,Dennis Brown,Sylvie Breton

    Epithelial cells are immune sensors and mediators that constitute the first line of defense against infections. Using the epididymis, a model for studying tubular organs, we uncovered a novel and unexpected role for professional proton-secreting 'clear cells' in sperm maturation and immune defense. The epididymal epithelium participates in the maturation of spermatozoa via the establishment of an acidic

    更新日期:2020-03-16
  • Differential nanoscale organisation of LFA-1 modulates T-cell migration.
    J. Cell Sci. (IF 4.517) Pub Date : 2019-09-01
    Michael J Shannon,Judith Pineau,Juliette Griffié,Jesse Aaron,Tamlyn Peel,David J Williamson,Rose Zamoyska,Andrew P Cope,Georgina H Cornish,Dylan M Owen

    Effector T-cells rely on integrins to drive adhesion and migration to facilitate their immune function. The heterodimeric transmembrane integrin LFA-1 (αLβ2 integrin) regulates adhesion and migration of effector T-cells through linkage of the extracellular matrix with the intracellular actin treadmill machinery. Here, we quantified the velocity and direction of F-actin flow in migrating T-cells alongside

    更新日期:2020-03-16
  • Myo1e modulates the recruitment of activated B cells to inguinal lymph nodes.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-03-02
    Daniel A Girón-Pérez,Eduardo Vadillo,Michael Schnoor,Leopoldo Santos-Argumedo

    The inclusion of lymphocytes in high endothelial venules and their migration to the lymph nodes are critical steps in the immune response. Cell migration is regulated by the actin cytoskeleton and myosins. Myo1e is a long-tailed class I myosin and is highly expressed in B cells, which have not been studied in the context of cell migration. By using intravital microscopy in an in vivo model and performing

    更新日期:2020-03-16
  • B cells rapidly target antigen and surface-derived MHCII into peripheral degradative compartments.
    J. Cell Sci. (IF 4.517) Pub Date : 2019-12-20
    Sara Hernández-Pérez,Marika Vainio,Elina Kuokkanen,Vid Šuštar,Petar Petrov,Sofia Forstén,Vilma Paavola,Johanna Rajala,Luqman O Awoniyi,Alexey V Sarapulov,Helena Vihinen,Eija Jokitalo,Andreas Bruckbauer,Pieta K Mattila

    In order to mount high-affinity antibody responses, B cells internalise specific antigens and process them into peptides loaded onto MHCII for presentation to T helper cells (TH cells). While the biochemical principles of antigen processing and MHCII loading have been well dissected, how the endosomal vesicle system is wired to enable these specific functions remains much less studied. Here, we performed

    更新日期:2020-03-16
  • A new role for Notch in the control of polarity and asymmetric cell division of developing T cells.
    J. Cell Sci. (IF 4.517) Pub Date : 2019-10-04
    Mirren Charnley,Mandy Ludford-Menting,Kim Pham,Sarah M Russell

    A fundamental question in biology is how single cells can reliably produce progeny of different cell types. Notch signalling frequently facilitates fate determination. Asymmetric cell division (ACD) often controls segregation of Notch signalling by imposing unequal inheritance of regulators of Notch. Here, we assessed the functional relationship between Notch and ACD in mouse T cell development. To

    更新日期:2020-03-16
  • RHOA-mediated mechanical force generation through Dectin-1.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-03-02
    Rohan P Choraghe,Tomasz Kołodziej,Alan Buser,Zenon Rajfur,Aaron K Neumann

    Dendritic cell-associated C-type lectin 1 (Dectin-1, also known as CLEC7A) is an innate immune pattern recognition receptor that recognizes β-glucan on the Candida albicans cell wall. Recognition of β-glucan by immune cells leads to phagocytosis, oxidative burst, cytokine and chemokine production. We looked for specific mechanisms that coordinate phagocytosis downstream of Dectin-1 leading to actin

    更新日期:2020-03-16
  • Discovery of aspirin-triggered eicosanoid-like mediators in a Drosophila metainflammation blood tumor model.
    J. Cell Sci. (IF 4.517) Pub Date : 2019-10-28
    Silvio Panettieri,Indira Paddibhatla,Jennifer Chou,Roma Rajwani,Rebecca S Moore,Tamara Goncharuk,George John,Shubha Govind

    Epidemiologic studies have linked the use of aspirin to a decline in chronic inflammation that underlies many human diseases, including some cancers. Aspirin reduces the levels of cyclooxygenase-mediated pro-inflammatory prostaglandins, promotes the production of pro-resolution molecules, and triggers the production of anti-inflammatory electrophilic mono-oxygenated (EFOX) lipid mediators. We investigated

    更新日期:2020-03-16
  • FcRn mediates fast recycling of endocytosed albumin and IgG from early macropinosomes in primary macrophages.
    J. Cell Sci. (IF 4.517) Pub Date : 2019-08-25
    Wei Hong Toh,Jade Louber,Ismail S Mahmoud,Jenny Chia,Greg T Bass,Steve K Dower,Anne M Verhagen,Paul A Gleeson

    The neonatal Fc receptor (FcRn) rescues albumin and IgG from degradation following endocytosis and thereby extends the half-life of these plasma proteins. However, the pathways for the uptake of these soluble FcRn ligands, and the recycling itinerary of the FcRn-ligand complexes, have not been identified in primary cells. Here, we have defined the recycling of human albumin and IgG in primary mouse

    更新日期:2020-03-16
  • Live imaging the foreign body response in zebrafish reveals how dampening inflammation reduces fibrosis.
    J. Cell Sci. (IF 4.517) Pub Date : 2019-08-25
    David B Gurevich,Kathryn E French,John D Collin,Stephen J Cross,Paul Martin

    Implanting biomaterials in tissues leads to inflammation and a foreign body response (FBR), which can result in rejection. Here, we live image the FBR triggered by surgical suture implantation in a translucent zebrafish model and compare with an acute wound response. We observe inflammation extending from the suture margins, correlating with subsequent avascular and fibrotic encapsulation zones: sutures

    更新日期:2020-03-16
  • Reactive oxygen species-mediated cytoplasmic stiffening impairs the phagocytic ability of the macrophage.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-01-31
    Mahesh Agarwal,Parijat Biswas,Anindita Bhattacharya,Deepak Kumar Sinha

    The phagocytic ability of macrophages empowers them to enforce innate immunity. RAW264.7, THP-1 and peripheral blood mononuclear cell-derived macrophages display considerable variability with regards to their phagocytic ability. We identify the underlying causes that attenuate the phagocytic abilities of a macrophage. Deformability of the cytoplasm and cortex influences the macrophage's phagocytic

    更新日期:2020-03-16
  • Neutrophil phagocyte oxidase activity controls invasive fungal growth and inflammation in zebrafish.
    J. Cell Sci. (IF 4.517) Pub Date : 2019-12-20
    Taylor J Schoen,Emily E Rosowski,Benjamin P Knox,David Bennin,Nancy P Keller,Anna Huttenlocher

    Neutrophils are primary phagocytes of the innate immune system that generate reactive oxygen species (ROS) and mediate host defense. Deficient phagocyte NADPH oxidase (PHOX) function leads to chronic granulomatous disease (CGD) that is characterized by invasive infections, including those by the generally non-pathogenic fungus Aspergillus nidulans The role of neutrophil ROS in this specific host-pathogen

    更新日期:2020-03-16
  • Rat cytomegalovirus-encoded γ-chemokine vXCL1 is a highly adapted, species-specific agonist for rat XCR1-positive dendritic cells.
    J. Cell Sci. (IF 4.517) Pub Date : 2019-10-28
    Agnieszka Bauer,Julia Madela,Christian Berg,Viktorija Daugvilaite,Stephanie Gurka,Hans Werner Mages,Richard A Kroczek,Mette M Rosenkilde,Sebastian Voigt

    Dendritic cells (DCs) expressing the chemokine receptor XCR1 are specialized in antigen cross-presentation to control infections with intracellular pathogens. XCR1-positive (XCR1+) DCs are attracted by XCL1, a γ-chemokine secreted by activated CD8+ T cells and natural killer cells. Rat cytomegalovirus (RCMV) is the only virus known to encode a viral XCL1 analog (vXCL1) that competes for XCR1 binding

    更新日期:2020-03-16
  • TLR7 trafficking and signaling in B cells is regulated by the MHCII-associated invariant chain.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-20
    Mira Tohmé,Lucie Maisonneuve,Karim Achour,Michaël Dussiot,Sophia Maschalidi,Bénédicte Manoury

    Toll-like receptor 7 (TLR7) is an endosomal receptor, which recognizes single-stranded RNA from viruses. Its trafficking and activation is regulated by the endoplasmic reticulum chaperone UNC93B1 and lysosomal proteases. UNC93B1 also modulates major histocompatibility class II (MHCII) antigen presentation and deficiency in MHCII protein diminishes TLR9 signaling. These results indicate a link between

    更新日期:2020-03-16
  • Ca2+ activated cleavage of ezrin visualised dynamically in living myeloid cells during "cell surface area expansion".
    J. Cell Sci. (IF 4.517) Pub Date : 2020-01-13
    Rhiannon E Roberts,Marianne Martin,Sabrina Marion,Geetha L Elumalai,Kimberly Lewis,Maurice B Hallett

    The intracellular events underlying phagocytosis, a crucial event for innate immunity, are still unresolved. In order to test whether the reservoir of membrane for the formation of the phagocytic pseudopodia is maintained by cortical ezrin, and that its cleavage is a key step in releasing this membrane, the cleavage of cortical ezrin was monitored within living phagocytes (phagocytically-competent

    更新日期:2020-03-16
  • Epidermal growth factor receptor signaling suppresses αvβ6 integrin and promotes periodontal inflammation and bone loss.
    J. Cell Sci. (IF 4.517) Pub Date : null
    Jiarui Bi,Leeni Koivisto,Jiayin Dai,Deshu Zhuang,Guoqiao Jiang,Milla Larjava,Ya Shen,Liangjia Bi,Fang Liu,Markus Haapasalo,Lari Häkkinen,Hannu Larjava

    In periodontal disease (PD), bacterial biofilms cause gingival inflammation leading to bone loss. In health, αvβ6 integrin in junctional epithelium maintains anti-inflammatory transforming growth factor-β1 (TGF-β1) signaling, whereas its expression is lost in PD. Bacterial biofilms suppress β6 integrin expression in cultured gingival epithelial cells (GECs) by attenuating TGF-β1 signaling, leading

    更新日期:2020-03-16
  • IL-15Rα membrane-anchorage either in cis or in trans is required for stabilization of IL-15 and optimal signaling.
    J. Cell Sci. (IF 4.517) Pub Date : null
    Agnès Quéméner,Sébastien Morisseau,Rui P Sousa,Kilian Trillet,Mike Maillasson,Isabelle Leray,Yannick Jacques,Johann Dion,Isabelle Barbieux,Marie Frutoso,Adèle D Laurent,Jean-Yves Le Questel,Erwan Mortier

    Interleukin(IL)-15 plays an important role in the communication between immune cells. IL-15 delivers its signal through different modes involving three receptor chains: IL-15Rα, IL-2Rβ and γc. The combination of the different chains result in the formation of IL-15Rα/IL-2Rβ/γc trimeric or IL-2Rβ/γc dimeric receptors. In this study, we aimed at investigating the role of IL-15Rα chain in stabilizing

    更新日期:2020-03-16
  • P2X7 receptor activation increases caveolin-1 expression and macrophage lipid raft formation boosting CD39 activity.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-01-31
    Luiz Eduardo Baggio Savio,Paola de Andrade Mello,Stephanie Alexia Cristina Silva Santos,Júlia Costa de Sousa,Suellen D S Oliveira,Richard D Minshall,Eleonora Kurtenbach,Yan Wu,Maria Serena Longhi,Simon C Robson,Robson Coutinho-Silva

    Macrophages are tissue-resident immune cells crucial for the initiation and maintenance of immune responses. Purinergic signaling modulates macrophage activity and impacts cellular plasticity. The ATP-activated purinergic receptor P2X7 has pro-inflammatory properties, which contribute to macrophage activation. P2X7 receptor signaling is in turn modulated by ectonucleotidases such as CD39/E-NTPDase1

    更新日期:2020-03-16
  • Identification of serine residues in the Connexin43 carboxyl tail important for BCR-mediated spreading of B-lymphocytes.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-05
    Farnaz Pournia,May Dang-Lawson,Kate Choi,Victor Mo,Paul D Lampe,Linda Matsuuchi

    B-lymphocytes recognize antigen via B cell antigen receptors (BCR). This binding induces signaling leading to B cell activation, proliferation and differentiation. Early events of BCR signaling include reorganization of actin, and membrane spreading for increased antigen gathering. We have previously shown that the gap junction protein connexin43 (Cx43) is phosphorylated upon BCR signaling, and its

    更新日期:2020-03-16
  • Loss of Ca2+ entry via Orai-TRPC1 induces ER stress, initiating immune activation in macrophages.
    J. Cell Sci. (IF 4.517) Pub Date : 2019-12-18
    Viviane Nascimento Da Conceicao,Yuyang Sun,Emily K Zboril,Jorge J De la Chapa,Brij B Singh

    Activation of cellular stresses is associated with inflammation; however, the mechanisms are not well identified. Here, we provide evidence that loss of Ca2+ influx induces endoplasmic reticulum (ER) stress in primary macrophages and in murine macrophage cell line Raw 264.7, in which the unfolded protein response is initiated to modulate cytokine production, thereby activating the immune response.

    更新日期:2020-03-16
  • The Lifeact-EGFP mouse is a translationally controlled fluorescent reporter of T cell activation.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-10
    Jorge Luis Galeano Niño,Szun S Tay,Jacqueline L E Tearle,Jianling Xie,Matt A Govendir,Daryan Kempe,Jessica Mazalo,Alexander P Drew,Feyza Colakoglu,Sarah K Kummerfeld,Christopher G Proud,Maté Biro

    It has become increasingly evident that T cell functions are subject to translational control in addition to transcriptional regulation. Live imaging of CD8+ T cells isolated from the Lifeact-EGFP mouse reveals that they exhibit a gain in fluorescence intensity following engagement of cognate tumour target cells. The GFP signal increase is governed by Erk-dependent distal TCR signalling and its magnitude

    更新日期:2020-03-16
  • Pan-SHIP1/2 inhibitors promote microglia effector functions essential for CNS homeostasis.
    J. Cell Sci. (IF 4.517) Pub Date : null
    C Pedicone,S Fernandes,O M Dungan,S M Dormann,D R Viernes,A A Adhikari,L B Choi,E P De Jong,J D Chisholm,W G Kerr

    We show here that both SHIP1 and its paralog SHIP2 are expressed at the protein level in microglia. To examine whether targeting of SHIP paralogs might influence microglial physiology and function we tested the capacity of SHIP1-selective, SHIP2-selective and pan-SHIP1/2 inhibitors for their ability to impact microglia proliferation, lysosomal compartment size and phagocytic function. We find that

    更新日期:2020-03-16
  • Cofilin1 driven actin dynamics controls migration of thymocytes and is essential for positive selection in the thymus.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-01-23
    Andree Salz,Christine Gurniak,Friederike Jönsson,Walter Witke

    Actin dynamics is essential for T cell development. We show here that cofilin1 is the key molecule for controlling actin filament turnover in this process. Mice with specific depletion of cofilin1 in thymocytes showed increased steady state levels of actin filaments, and associated alterations in the pattern of thymocyte migration and adhesion. Our data suggest that cofilin1 is controlling oscillatory

    更新日期:2020-03-16
  • Vav2 lacks calcium entry-promoting scaffolding functions unique to Vav1 and inhibits T cell activation via Cdc42.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-01-23
    Michael A Fray,John C Charpentier,Nicholas R Sylvain,Maria-Cristina Seminario,Stephen C Bunnell

    Vav family guanine nucleotide exchange factors (GEFs) are essential regulators of immune function. Despite their structural similarity, Vav1 promotes and Vav2 opposes T cell receptor (TCR)-induced calcium entry. Using a Vav1-deficient Jurkat T cell line, we find that Vav1 facilitates calcium entry via non-catalytic scaffolding functions that are encoded by the catalytic core of Vav1 and flanking linker

    更新日期:2020-03-16
  • In vivo interactions between myosin XI, vesicles and filamentous actin are fast and transient in Physcomitrella patens.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-26
    Jeffrey P Bibeau,Fabienne Furt,S Iman Mousavi,James L Kingsley,Max F Levine,Erkan Tüzel,Luis Vidali

    The actin cytoskeleton and active membrane trafficking machinery are essential for polarized cell growth. To understand the interactions between myosin XI, vesicles and actin filaments in vivo, we performed fluorescence recovery after photobleaching and showed that the dynamics of myosin XIa at the tip of the spreading earthmoss Physcomitrella patens caulonemal cells are actin-dependent and that 50%

    更新日期:2020-03-02
  • Security
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-17
    Mole,

    Mole

    更新日期:2020-03-02
  • Cep44 functions in centrosome cohesion by stabilizing rootletin.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-01-23
    Delowar Hossain,Sunny Y-P Shih,Xintong Xiao,Julia White,William Y Tsang

    The centrosome linker serves to hold the duplicated centrosomes together until they separate in late G2/early mitosis. Precisely how the linker is assembled remains an open question. In this study, we identify Cep44 as a novel component of the linker in human cells. Cep44 localizes to the proximal end of centrioles, including mother and daughter centrioles, and its ablation leads to loss of centrosome

    更新日期:2020-03-02
  • The gap junction protein connexin 43 controls multiple aspects of cranial neural crest cell development.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-20
    Karyn Jourdeuil,Lisa A Taneyhill

    Gap junctions are intercellular channels between cells that facilitate cell-cell communication. Connexin 43 (Cx43; also known as GJA1), the predominant gap junction protein in vertebrates, is expressed in premigratory cranial neural crest cells and is maintained throughout the neural crest cell epithelial-to-mesenchymal transition (EMT), but its function in these cells is unknown. To this end, we used

    更新日期:2020-03-02
  • CSB cooperates with SMARCAL1 to maintain telomere stability in ALT cells.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-01-23
    Emily Feng,Nicole L Batenburg,John R Walker,Angus Ho,Taylor R H Mitchell,Jian Qin,Xu-Dong Zhu

    Elevated replication stress is evident at telomeres of about 10-15% of cancer cells, which maintain their telomeres via a homologous recombination (HR)-based mechanism, referred to as alternative lengthening of telomeres (ALT). How ALT cells resolve replication stress to support their growth remains incompletely characterized. Here, we report that CSB (also known as ERCC6) promotes recruitment of HR

    更新日期:2020-03-02
  • Multivalent nephrin-Nck interactions define a threshold for clustering and tyrosine-dependent nephrin endocytosis
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-24
    Martin, C. E., New, L. A., Phippen, N. J., Keyvani Chahi, A., Mitro, A. E., Takano, T., Pawson, T., Blasutig, I. M., Jones, N.

    Claire E. Martin, Laura A. New, Noah J. Phippen, Ava Keyvani Chahi, Alexander E. Mitro, Tomoko Takano, Tony Pawson, Ivan M. Blasutig, and Nina Jones Assembly of signaling molecules into micrometer-sized clusters is driven by multivalent protein-protein interactions, such as those found within the nephrin–Nck (Nck1 or Nck2) complex. Phosphorylation on multiple tyrosine residues within the tail of the

    更新日期:2020-03-02
  • A potent nuclear export mechanism imposes USP16 cytoplasmic localization during interphase
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-24
    Sen Nkwe, N., Daou, S., Uriarte, M., Gagnon, J., Iannantuono, N. V., Barbour, H., Yu, H., Masclef, L., Fernandez, E., Zamorano Cuervo, N., Mashtalir, N., Binan, L., Sergeev, M., Belanger, F., Drobetsky, E., Milot, E., Wurtele, H., Costantino, S., Affar, E. B.

    Nadine Sen Nkwe, Salima Daou, Maxime Uriarte, Jessica Gagnon, Nicholas Victor Iannantuono, Haithem Barbour, Helen Yu, Louis Masclef, Erlinda Fernandez, Natalia Zamorano Cuervo, Nazar Mashtalir, Loïc Binan, Mikhail Sergeev, Francois Belanger, Elliot Drobetsky, Eric Milot, Hugo Wurtele, Santiago Costantino, and El Bachir Affar USP16 (also known as UBP-M) has emerged as a histone H2AK119 deubiquitylase

    更新日期:2020-03-02
  • Lamin A/C modulates spatial organization and function of the Hsp70 gene locus via nuclear myosin I.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-21
    Roopali Pradhan,Muhunden Jayakrishnan Nallappa,Kundan Sengupta

    The structure-function relationship of the nucleus is tightly regulated, especially during heat shock. Typically, heat shock activates molecular chaperones that prevent protein misfolding and preserve genome integrity. However, the molecular mechanisms that regulate nuclear structure-function relationships during heat shock remain unclear. Here, we show that lamin A and C (hereafter lamin A/C; both

    更新日期:2020-03-02
  • Recycling endosomes attach to the trans-side of Golgi stacks in Drosophila and mammalian cells.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-01-23
    Syara Fujii,Kazuo Kurokawa,Ryota Inaba,Naoki Hiramatsu,Tatsuya Tago,Yuri Nakamura,Akihiko Nakano,Takunori Satoh,Akiko K Satoh

    Historically, the trans-Golgi network (TGN) has been recognized as a sorting center of newly synthesized proteins, whereas the recycling endosome (RE) is a compartment where endocytosed materials transit before being recycled to the plasma membrane. However, recent findings revealed that both the TGN and RE connect endocytosis and exocytosis and, thus, are functionally overlapping. Here we report,

    更新日期:2020-03-02
  • "If you please… draw me a cell". Insights from immune cells.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-03-02
    Hélène D Moreau,Ana-Maria Lennon-Duménil,Paolo Pierobon

    Studies in recent years have shed light on the particular features of cytoskeleton dynamics in immune cells, challenging the classical picture drawn from typical adherent cell lines. New mechanisms linking the dynamics of the membrane-cytoskeleton interface to the mechanical properties of immune cells have been uncovered and shown to be essential for immune surveillance functions. In this Essay, we

    更新日期:2020-03-02
  • Plakophilin 3 phosphorylation by ribosomal S6 kinase supports desmosome assembly.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-03-02
    Lisa Müller,Katrin Rietscher,René Keil,Marvin Neuholz,Mechthild Hatzfeld

    Desmosome remodeling is crucial for epidermal regeneration, differentiation and wound healing. It is mediated by adapting the composition and by posttranslational modifications of constituent proteins. We have previously demonstrated that plakophilin 1 (PKP1) mediates strong adhesion in suprabasal keratinocytes which is negatively regulated by insulin-like growth factor-1 (IGF1) signaling. The importance

    更新日期:2020-03-02
  • Direct interaction between CEP85 and STIL mediates PLk4-driven directed cell migration.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-27
    Yi Liu,Jaeyoun Kim,Reuben Philip,Vaishali Sridhar,Megha Chandrashekhar,Jason Moffat,Mark van Breugel,Laurence Pelletier

    PLK4 has emerged as a prime target for cancer therapeutics and its overexpression is frequently observed in various types of human cancer. Recent studies have further revealed an unexpected oncogenic activity of PLK4 in regulating cancer cell migration and invasion. However, the molecular basis behind PLK4's role in these processes still remains only partly understood. Our previous work demonstrated

    更新日期:2020-02-27
  • Regenerative responses following DNA damage: β-catenin mediates head regrowth in the planarian Schmidtea mediterranea.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-27
    Annelies Wouters,Jan-Pieter Ploem,Sabine A S Langie,Tom Artois,Aziz Aboobaker,Karen Smeets

    Pluripotent stem cells hold great potential for regenerative medicine. Increased replication and division, such is the case during regeneration, concomitantly increases the risk of adverse outcomes through the acquisition of mutations. Seeking for driving mechanisms of such outcomes, we challenged a pluripotent stem cell system during the tightly controlled regeneration process in the planarian Schmidtea

    更新日期:2020-02-27
  • FAK regulates actin polymerization during capacitation via the ERK2/GEF- H1/RhoA signaling pathway.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-27
    Monica L Salgado-Lucio,Danelia Ramírez-Ramírez,Coral Y Jorge-Cruz,Ana L Roa-Espitia,Enrique O Hernández-González

    Actin polymerization is a crucial process during capacitation. We have recently described the participation of FAK in actin polymerization in guinea pig spermatozoa. However, the mechanism by which FAK achieves these processes is unknown. Our data showed that ERK2 is activated during the first minutes of capacitation; its inhibition blocked actin polymerization and the acrosome reaction. Additionally

    更新日期:2020-02-27
  • STIM1 interacts with termini of Orai channels in a sequential manner.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-27
    Liling Niu,Fuyun Wu,Kaili Li,Jing Li,Shenyuan L Zhang,Junjie Hu,Qian Wang

    Store-operated calcium entry (SOCE) is critical for numerous calcium-related processes. The activation of SOCE requires engagement between stromal interaction molecule 1 (STIM1) molecules on the endoplasmic reticulum and Ca2+ release-activated Ca2+ channel (CRAC) Orai on the plasma membrane. However, the molecular details of their interactions remain elusive. Here, we analyzed STIM1-Orai interactions

    更新日期:2020-02-27
  • Spatio-temporal control of post-Golgi exocytic trafficking in plants.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-26
    Liam Elliott,Ian Moore,Charlotte Kirchhelle

    A complex and dynamic endomembrane system is a hallmark of eukaryotic cells and underpins the evolution of specialised cell types in multicellular organisms. Endomembrane system function critically depends on the ability of the cell to (1) define compartment and pathway identity, and (2) organise compartments and pathways dynamically in space and time. Eukaryotes possess a complex molecular machinery

    更新日期:2020-02-26
  • AstroDot: a new method for studying the spatial distribution of mRNA in astrocytes.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-25
    Marc Oudart,Romain Tortuyaux,Philippe Mailly,Noémie Mazaré,Anne-Cécile Boulay,Martine Cohen-Salmon

    Astrocytes are morphologically complex and use local translation to regulate distal functions. To study the distribution of mRNA in astrocytes, we combined mRNA detection via in situ hybridization with immunostaining of the astrocyte-specific intermediate filament glial fibrillary acidic protein (GFAP). mRNAs at the level of GFAP-immunolabelled astrocyte somata, large and fine processes were analyzed

    更新日期:2020-02-25
  • Molecular regulation of the plasma membrane-proximal cellular steps involved in NK cell cytolytic function.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-21
    Prasad V Phatarpekar,Daniel D Billadeau

    Natural killer (NK) cells, cytolytic lymphocytes of the innate immune system, play a crucial role in the immune response against infection and cancer. NK cells kill target cells through exocytosis of lytic granules that contain cytotoxic proteins, such as perforin and granzymes. Formation of a functional immune synapse, i.e. the interface between the NK cell and its target cell enhances lysis through

    更新日期:2020-02-21
  • Optimizing live-cell fluorescence imaging conditions to minimize phototoxicity.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-21
    Alex Kiepas,Elena Voorand,Firas Mubaid,Peter M Siegel,Claire M Brown

    Fluorescence illumination can cause phototoxicity that negatively affects living samples. This study demonstrates that much of the phototoxicity and photobleaching experienced with live-cell fluorescence imaging occurs as a result of 'illumination overhead' (IO). This occurs when a sample is illuminated but fluorescence emission is not being captured by the microscope camera. Several technological

    更新日期:2020-02-21
  • Cell invasion by intracellular parasites - the many roads to infection.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-20
    Maria Fátima Horta,Luciana Oliveira Andrade,Érica Santos Martins-Duarte,Thiago Castro-Gomes

    Intracellular parasites from the genera Toxoplasma, Plasmodium, Trypanosoma, Leishmania and from the phylum Microsporidia are, respectively, the causative agents of toxoplasmosis, malaria, Chagas disease, leishmaniasis and microsporidiosis, illnesses that kill millions of people around the globe. Crossing the host cell plasma membrane (PM) is an obstacle these parasites must overcome to establish themselves

    更新日期:2020-02-20
  • PTPN23 binds the dynein adaptor BICD1 and is required for endocytic sorting of neurotrophin receptors.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-20
    Marta I Budzinska,David Villarroel-Campos,Matthew Golding,Anne Weston,Lucy Collinson,Ambrosius P Snijders,Giampietro Schiavo

    Signalling by target-derived neurotrophins is essential for the correct development of the nervous system and its maintenance throughout life. Several aspects concerning the lifecycle of neurotrophins and their receptors have been characterised over the years, including formation of signalling-competent ligand-receptor complexes, their endocytosis and trafficking. However, the molecular mechanisms

    更新日期:2020-02-20
  • Regulation of intrinsic polarity establishment by a differentiation-type MAPK pathway.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-20
    Aditi Prabhakar,Jacky Chow,Alan J Siegel,Paul J Cullen

    All cells establish and maintain an axis of polarity that is critical for cell shape and progression through the cell cycle. A well-studied example of polarity establishment is bud emergence in yeast, which is controlled by the Rho GTPase Cdc42p. The prevailing view of bud emergence does not account for regulation by extrinsic cues. Here, we show that the filamentous growth MAPK pathway (fMAPK) regulates

    更新日期:2020-02-20
  • Distinct roles of two eIF4E isoforms in the germline of Caenorhabditis elegans.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-20
    Hayden P Huggins,Jacob S Subash,Hamilton Stoffel,Melissa A Henderson,Jenna L Hoffman,David S Buckner,Madhu S Sengupta,Peter R Boag,Myon-Hee Lee,Brett D Keiper

    Germ cells use both positive and negative mRNA translational control to regulate gene expression that drives their differentiation into gametes. mRNA translational control is mediated by RNA-binding proteins, miRNAs, and translation initiation factors. We have uncovered the discrete roles of two translation initiation factor eIF4E isoforms (IFE-1 and -3) that bind m7GTP mRNA caps during C. elegans

    更新日期:2020-02-20
  • Increased host ATP efflux and its conversion to extracellular adenosine is crucial for establishing Leishmania infection.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-20
    Moumita Basu,Purnima Gupta,Ananya Dutta,Kuladip Jana,Anindita Ukil

    Intracellular survival of Leishmania donovani demands rapid production of host ATP for its sustenance. However, gradual decrease in intracellular ATP in spite of increased glycolysis suggested ATP efflux during infection. Accordingly, extracellular ATP showed an increase and pannexin-1 was found to be the major ATP exporter in infected condition. Extracellular ATP also showed a gradual decrease after

    更新日期:2020-02-20
  • Arp2/3 nucleates F-actin coating of fusing insulin granules in pancreatic β cells to control insulin secretion.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-20
    Wei Ma,Jenny Chang,Jason Tong,Uda Ho,Belinda Yau,Melkam A Kebede,Peter Thorn

    F-actin dynamics are known to control insulin secretion but the point of intersection with the stimulus-secretion cascade is unknown. Here, using multiphoton imaging of β cells isolated from Lifeact-GFP transgenic mice, we show glucose stimulation does not cause global changes in subcortical F-actin. Instead, we observe spatially discrete and transient F-actin changes around each fusing granule. This

    更新日期:2020-02-20
  • A DNA polymerization-independent role for mitochondrial DNA polymerase IC in African trypanosomes.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-20
    Jonathan C Miller,Stephanie B Delzell,Jeniffer Concepción-Acevedo,Michael J Boucher,Michele M Klingbeil

    The mitochondrial DNA of Trypanosoma brucei and related parasites is a catenated network containing thousands of minicircles and tens of maxicircles called kinetoplast DNA (kDNA). Replication of the single nucleoid requires at least three DNA polymerases (POLIB, POLIC, and POLID) each having discrete localization near the kDNA during S phase. POLIB and POLID have roles in minicircle replication while

    更新日期:2020-02-20
  • Non-canonical regulation of homologous recombination DNA repair by the USP9X deubiquitylase.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-12
    Rachel O'Dea,Corrado Santocanale

    In order to prevent the deleterious effects of genotoxic agents, cells have developed complex surveillance mechanisms and DNA repair pathways that allow them to maintain genome integrity. The ubiquitin-specific protease 9X (USP9X) contributes to genome stability during DNA replication and chromosome segregation. Depletion of USP9X leads to DNA double-strand breaks, some of which are triggered by replication

    更新日期:2020-02-18
  • Cooperative recruitment of Arl4A and Pak1 to the plasma membrane contributes to sustained Pak1 activation for cell migration.
    J. Cell Sci. (IF 4.517) Pub Date : 2020-02-04
    Kuan-Jung Chen,Tsai-Chen Chiang,Chia-Jung Yu,Fang-Jen S Lee

    Cell migration requires the coordination of multiple signaling pathways involved in membrane dynamics and cytoskeletal rearrangement. The Arf-like small GTPase Arl4A has been shown to modulate actin cytoskeleton remodeling. However, evidence of the function of Arl4A in cell migration is insufficient. Here, we report that Arl4A acts with the serine/threonine protein kinase Pak1 to modulate cell migration

    更新日期:2020-02-18
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