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Bioprinting of human pluripotent stem cell derived corneal endothelial cells with hydrazone crosslinked hyaluronic acid bioink Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-03-14 Pyry Grönroos, Anni Mörö, Paula Puistola, Karoliina Hopia, Maija Huuskonen, Taina Viheriälä, Tanja Ilmarinen, Heli Skottman
Human corneal endothelial cells lack regenerative capacity through cell division in vivo. Consequently, in the case of trauma or dystrophy, the only available treatment modality is corneal tissue or primary corneal endothelial cell transplantation from cadaveric donor which faces a high global shortage. Our ultimate goal is to use the state-of-the-art 3D-bioprint technology for automated production
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Effectiveness of extracellular vesicles derived from hiPSCs in repairing hyperoxia-induced injury in a fetal murine lung explant model Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-03-14 Hala Saneh, Heather Wanczyk, Joanne Walker, Christine Finck
Despite advances in neonatal care, the incidence of Bronchopulmonary Dysplasia (BPD) remains high among preterm infants. Human induced pluripotent stem cells (hiPSCs) have shown promise in repairing injury in animal BPD models. Evidence suggests they exert their effects via paracrine mechanisms. We aim herein to assess the effectiveness of extracellular vesicles (EVs) derived from hiPSCs and their
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Transplanted human photoreceptors transfer cytoplasmic material but not to the recipient mouse retina Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-03-14 Margaret T. Ho, Kotoe Kawai, Dhana Abdo, Lacrimioara Comanita, Arturo Ortin-Martinez, Yui Ueno, Emily Tsao, Azam Rastgar-Moghadam, Chang Xue, Hong Cui, Valerie A. Wallace, Molly S. Shoichet
The discovery of material transfer between transplanted and host mouse photoreceptors has expanded the possibilities for utilizing transplanted photoreceptors as potential vehicles for delivering therapeutic cargo. However, previous research has not directly explored the capacity for human photoreceptors to engage in material transfer, as human photoreceptor transplantation has primarily been investigated
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Cholangiocyte organoids to study drug-induced injury Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-03-13 Zhenguo Wang, Chen Xing, Luc J. W. van der Laan, Monique M. A. Verstegen, Bart Spee, Rosalinde Masereeuw
Drug induced bile duct injury is a frequently observed clinical problem leading to a wide range of pathological features. During the past decades, several agents have been identified with various postulated mechanisms of bile duct damage, however, mostly still poorly understood. Here, we investigated the mechanisms of chlorpromazine (CPZ) induced bile duct injury using advanced in vitro cholangiocyte
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Transcriptomic and proteomic profiles of fetal versus adult mesenchymal stromal cells and mesenchymal stromal cell-derived extracellular vesicles Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-03-13 Emine Begüm Gençer, Yuk Kit Lor, Fawaz Abomaray, Samir El Andaloussi, Maria Pernemalm, Nidhi Sharma, Daniel W Hagey, André Görgens, Manuela O Gustafsson, Katarina Le Blanc, Mawaddah Asad Toonsi, Lilian Walther-Jallow, Cecilia Götherström
Mesenchymal stem/stromal cells (MSCs) can regenerate tissues through engraftment and differentiation but also via paracrine signalling via extracellular vesicles (EVs). Fetal-derived MSCs (fMSCs) have been shown, both in vitro and in animal studies, to be more efficient than adult MSC (aMSCs) in generating bone and muscle but the underlying reason for this difference has not yet been clearly elucidated
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Short cell cycle duration is a phenotype of human epidermal stem cells Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-03-13 Tong Xiao, Ugomma C Eze, Alex Charruyer-Reinwald, Tracy Weisenberger, Ayman Khalifa, Brook Abegaze, Gabrielle K Schwab, Rasha H Elsabagh, T. Richard Parenteau, Karl Kochanowski, Merisa Piper, Yumin Xia, Jeffrey B Cheng, Raymond J Cho, Ruby Ghadially
A traditional view is that stem cells (SCs) divide slowly. Meanwhile, both embryonic and pluripotent SCs display a shorter cell cycle duration (CCD) in comparison to more committed progenitors (CPs). We examined the in vitro proliferation and cycling behavior of somatic adult human cells using live cell imaging of passage zero keratinocytes and single-cell RNA sequencing. We found two populations of
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Menstrual blood-derived mesenchymal stem cells combined with collagen I gel as a regenerative therapeutic strategy for degenerated disc after discectomy in rats Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-03-13 Li Yu, Honghao Wu, Shumei Zeng, Xiaojian Hu, Yuxu Wu, Jinhong Zhou, Li Yuan, Qingqing Zhang, Charlie Xiang, Zhiyun Feng
Annulus fibrosis (AF) defects have been identified as the primary cause of disc herniation relapse and subsequent disc degeneration following discectomy. Stem cell-based tissue engineering offers a promising approach for structural repair. Menstrual blood-derived mesenchymal stem cells (MenSCs), a type of adult stem cell, have gained attention as an appealing source for clinical applications due to
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Deep learning-based predictive classification of functional subpopulations of hematopoietic stem cells and multipotent progenitors Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-03-13 Shen Wang, Jianzhong Han, Jingru Huang, Khayrul Islam, Yuheng Shi, Yuyuan Zhou, Dongwook Kim, Jane Zhou, Zhaorui Lian, Yaling Liu, Jian Huang
Hematopoietic stem cells (HSCs) and multipotent progenitors (MPPs) play a pivotal role in maintaining lifelong hematopoiesis. The distinction between stem cells and other progenitors, as well as the assessment of their functions, has long been a central focus in stem cell research. In recent years, deep learning has emerged as a powerful tool for cell image analysis and classification/prediction. In
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Pre-clinical evaluation of the efficacy and safety of human induced pluripotent stem cell-derived cardiomyocyte patch Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-03-13 Shigeru Miyagawa, Takuji Kawamura, Emiko Ito, Maki Takeda, Hiroko Iseoka, Junya Yokoyama, Akima Harada, Noriko Mochizuki-Oda, Yukiko Imanishi-Ochi, Junjun Li, Masao Sasai, Fumiyo Kitaoka, Masaki Nomura, Naoki Amano, Tomoko Takahashi, Hiromi Dohi, Eiichi Morii, Yoshiki Sawa
Cell- or tissue-based regenerative therapy is an attractive approach to treat heart failure. A tissue patch that can safely and effectively repair damaged heart muscle would greatly improve outcomes for patients with heart failure. In this study, we conducted a preclinical proof-of-concept analysis of the efficacy and safety of clinical-grade human induced pluripotent stem cell-derived cardiomyocyte
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Large-scale bioreactor production of extracellular vesicles from mesenchymal stromal cells for treatment of acute radiation syndrome Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-03-13 John A. Kink, Michael A. Bellio, Matthew H. Forsberg, Alexandra Lobo, Anna S. Thickens, Bryson M. Lewis, Irene M. Ong, Aisha Khan, Christian M. Capitini, Peiman Hematti
Hematopoietic acute radiation syndrome (H-ARS) occurring after exposure to ionizing radiation damages bone marrow causing cytopenias, increasing susceptibility to infections and death. We and others have shown that cellular therapies like human mesenchymal stromal cells (MSCs), or monocytes/macrophages educated ex-vivo with extracellular vesicles (EVs) from MSCs were effective in a lethal H-ARS mouse
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Cryopreserved cGMP-compliant human pluripotent stem cell-derived hepatic progenitors rescue mice from acute liver failure through rapid paracrine effects on liver cells Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-03-12 Malika Gantier, Raphaël Rispal, Angélique Fourrier, Séverine Ménoret, Frédéric Delbos, Ignacio Anegon, Tuan Huy Nguyen
Liver transplantation remains the only curative treatment for end-stage liver diseases. Unfortunately, there is a drastic organ donor shortage. Hepatocyte transplantation emerged as a viable alternative to liver transplantation. Considering their unique expansion capabilities and their potency to be driven toward a chosen cell fate, pluripotent stem cells are extensively studied as an unlimited cell
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PLOD2, a key factor for MRL MSC metabolism and chondroprotective properties Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-03-07 Sarah Bahraoui, Gautier Tejedor, Anne-Laure Mausset-Bonnefont, François Autelitano, Audrey Barthelaix, Claudia Terraza-Aguirre, Vincent Gisbert, Yoan Arribat, Christian Jorgensen, Mingxing Wei, Farida Djouad
Initially discovered for its ability to regenerate ear holes, the Murphy Roth Large (MRL) mouse has been the subject of multiple research studies aimed at evaluating its ability to regenerate other body tissues and at deciphering the mechanisms underlying it. These enhanced abilities to regenerate, retained during adulthood, protect the MRL mouse from degenerative diseases such as osteoarthritis (OA)
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Human umbilical cord/placenta mesenchymal stem cell conditioned medium attenuates intestinal fibrosis in vivo and in vitro Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-03-07 Yoon Jeong Choi, Woo Ram Kim, Duk Hwan Kim, Jee Hyun Kim, Jun Hwan Yoo
A significant unmet need in inflammatory bowel disease is the lack of anti-fibrotic agents targeting intestinal fibrosis. This study aimed to investigate the anti-fibrogenic properties and mechanisms of the conditioned medium (CM) from human umbilical cord/placenta-derived mesenchymal stem cells (UC/PL-MSC-CM) in a murine intestinal fibrosis model and human primary intestinal myofibroblasts (HIMFs)
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Mesenchymal stem/stromal cells from human pluripotent stem cell-derived brain organoid enhance the ex vivo expansion and maintenance of hematopoietic stem/progenitor cells Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-03-05 Ya Zhou, Xinping Cai, Xiuxiu Zhang, Yong Dong, Xu Pan, Mowen Lai, Yimeng Zhang, Yijin Chen, Xiaohong Li, Xia Li, Jiaxin Liu, Yonggang Zhang, Feng Ma
Mesenchymal stem/stromal cells (MSCs) are of great therapeutic value due to their role in maintaining the function of hematopoietic stem/progenitor cells (HSPCs). MSCs derived from human pluripotent stem cells represent an ideal alternative because of their unlimited supply. However, the role of MSCs with neural crest origin derived from HPSCs on the maintenance of HSPCs has not been reported. Flow
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Combined transplantation of hiPSC-NSC and hMSC ameliorated neuroinflammation and promoted neuroregeneration in acute spinal cord injury Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-03-05 Xiaofeng Du, Desheng Kong, Ruiyun Guo, Boxin Liu, Jingjing He, Jinyu Zhang, Asiamah Ernest Amponsah, Huixian Cui, Jun Ma
Spinal cord injury (SCI) is a serious clinical condition that has pathological changes such as increased neuroinflammation and nerve tissue damage, which eventually manifests as fibrosis of the injured segment and the development of a spinal cord cavity leading to loss of function. Cell-based therapy, such as mesenchymal stem cells (MSCs) and neural stem cells (NSCs) are promising treatment strategies
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Amelioration of diabetic nephropathy in mice by a single intravenous injection of human mesenchymal stromal cells at early and later disease stages is associated with restoration of autophagy Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-03-05 Jingjing He, Boxin Liu, Xiaofeng Du, Yan Wei, Desheng Kong, Baofeng Feng, Ruiyun Guo, Ernest Amponsah Asiamah, Matthew D. Griffin, Sean O. Hynes, Sanbing Shen, Yan Liu, Huixian Cui, Jun Ma, Timothy O’Brien
Mesenchymal stromal cells (MSCs) a potentially effective disease-modulating therapy for diabetic nephropathy (DN) but their clinical translation has been hampered by incomplete understanding of the optimal timing of administration and in vivo mechanisms of action. This study aimed to elucidate the reno-protective potency and associated mechanisms of single intravenous injections of human umbilical
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Impacts of priming on distinct immunosuppressive mechanisms of mesenchymal stromal cells under translationally relevant conditions Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-03-05 Nick Herger, Irina Heggli, Tamara Mengis, Jan Devan, Leonardo Arpesella, Florian Brunner, Oliver Distler, Stefan Dudli
The multimodal properties of mesenchymal stromal cells (MSCs), particularly their ability to modulate immune responses is of high interest in translational research. Pro-inflammatory, hypoxic, and 3D culture priming are promising and often used strategies to improve the immunosuppressive potency of MSCs, but the underlying mechanisms are not well understood. Therefore, the aims of this study were (i)
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Transplantation of the LRP1high subpopulation of human umbilical cord-derived mesenchymal stem cells improves ovarian function in mice with premature ovarian failure and aged mice Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-03-05 Jiacheng Shen, Li Wu, Xiaoying Shi, Gang Chen, Tingwei Liu, Fangfang Xu, Xiaocui Xu, Xiaochen Kou, Yanhong Zhao, Hong Wang, Chenfei Wang, Shaorong Gao, Shaohua Xu
Premature ovarian failure (POF) has a profound impact on female reproductive and psychological health. In recent years, the transplantation of umbilical cord-derived mesenchymal stem cells (UC-MSCs) has demonstrated unprecedented potential in the treatment of POF. However, the heterogeneity of human UC-MSCs remains a challenge for their large-scale clinical application. Therefore, it is imperative
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Embryonic stem cells overexpressing high molecular weight FGF2 isoform enhance recovery of pre-ganglionic spinal root lesion in combination with fibrin biopolymer mediated root repair Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-03-05 B. H. M. Lima, L. P. Cartarozzi, S. Kyrylenko, R. S. Ferreira, B. Barraviera, Alexandre L. R. Oliveira
Spinal ventral root avulsion results in massive motoneuron degeneration with poor prognosis and high costs. In this study, we compared different isoforms of basic fibroblast growth factor 2 (FGF2), overexpressed in stably transfected Human embryonic stem cells (hESCs), following motor root avulsion and repair with a heterologous fibrin biopolymer (HFB). In the present work, hESCs bioengineered to overexpress
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Evaluation of the efficacy of mesenchymal stem cells derived conditioned medium in the treatment of striae distensae: a double blind randomized clinical trial Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-03-05 Elham Behrangi, Masoomeh Feizollahi, Sona Zare, Azadeh Goodarzi, Mohammad Reza Ghasemi, Afsaneh Sadeghzadeh-Bazargan, Abbas Dehghani, Maryam Nouri, Roya Zeinali, Masoomeh Roohaninasab, Mohammad Ali Nilforoushzadeh
Striae distensae is a disfiguring atrophic skin condition that impairs the body’s aesthetic image. Despite the variety of conducted studies, there is controversy regarding the best modalities. Human mesenchymal stem cells are considered a rich source for scar treatment. Skin needling is among the most efficient and safe aesthetic and therapeutic devices. This study aimed to evaluate the efficacy of
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Culture of Hoffa fat pad mesenchymal stem/stromal cells on microcarrier suspension in vertical wheel bioreactor for extracellular vesicle production Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-03-05 Alexander Otahal, Karina Kramer, Markus Neubauer, Slavomira Gulová, Zsombor Lacza, Stefan Nehrer, Andrea De Luna
Mesenchymal stromal cells (MSCs) are increasingly employed in regenerative medicine approaches for their immunomodulatory and anti-inflammatory properties, which are encoded in their secretome including extracellular vesicles (EVs). The Hoffa fat pad (HFP) located infrapatellarly harbours MSCs that could assist in tissue homeostasis in osteoarthritic joints. Intraarticular injection therapies based
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Improving hematopoietic differentiation from human induced pluripotent stem cells by the modulation of Hippo signaling with a diarylheptanoid derivative Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-03-03 Umnuaychoke Thongsa-ad, Anongnat Wongpan, Wasinee Wongkummool, Phaewa Chaiwijit, Kwanchanok Uppakara, Gorawin Chaiyakitpattana, Passanan Singpant, Pirut Tong-ngam, Amnat Chukhan, Wachirachai Pabuprappap, Sirapope Wongniam, Apichart Suksamrarn, Suradej Hongeng, Usanarat Anurathapan, Kasem Kulkeaw, Alisa Tubsuwan, Kanit Bhukhai
The diarylheptanoid ASPP 049 has improved the quality of adult hematopoietic stem cell (HSC) expansion ex vivo through long-term reconstitution in animal models. However, its effect on hematopoietic regeneration from human induced pluripotent stem cells (hiPSCs) is unknown. We utilized a defined cocktail of cytokines without serum or feeder followed by the supplementation of ASPP 049 to produce hematopoietic
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Induced pluripotent stem cell derived pericytes respond to mediators of proliferation and contractility Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-03-03 Natalie E. King, Jo-Maree Courtney, Lachlan S. Brown, Alastair J. Fortune, Nicholas B. Blackburn, Jessica L. Fletcher, Jake M. Cashion, Jana Talbot, Alice Pébay, Alex W. Hewitt, Gary P. Morris, Kaylene M. Young, Anthony L. Cook, Brad A. Sutherland
Pericytes are multifunctional contractile cells that reside on capillaries. Pericytes are critical regulators of cerebral blood flow and blood–brain barrier function, and pericyte dysfunction may contribute to the pathophysiology of human neurological diseases including Alzheimers disease, multiple sclerosis, and stroke. Induced pluripotent stem cell (iPSC)-derived pericytes (iPericytes) are a promising
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Multiple treatments with human embryonic stem cell-derived mesenchymal progenitor cells preserved the fertility and ovarian function of perimenopausal mice undergoing natural aging Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-03-03 Eun-Young Shin, Suji Jeong, Jeoung Eun Lee, Dong Seok Jeong, Dong Keun Han, Seok-Ho Hong, Dong Ryul Lee
Currently, no approved stem cell-based therapies for preserving ovarian function during aging. To solve this problem, we developed a long-term treatment for human embryonic stem cell-derived mesenchymal progenitor cells (hESC-MPCs). We investigated whether the cells retained their ability to resist ovarian aging, which leads to delayed reproductive senescence. In a middle-aged female model undergoing
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Functional intestinal monolayers from organoids derived from human iPS cells for drug discovery research Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-29 Tatsuya Inui, Yusei Uraya, Jumpei Yokota, Tomoki Yamashita, Kanae Kawai, Kentaro Okada, Yukiko Ueyama-Toba, Hiroyuki Mizuguchi
Human induced pluripotent stem (iPS) cell-derived enterocyte-like cells (ELCs) are expected to be useful for evaluating the intestinal absorption and metabolism of orally administered drugs. However, it is difficult to generate large amounts of ELCs with high quality because they cannot proliferate and be passaged. To solve the issue above, we have established intestinal organoids from ELCs generated
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Impact of tissue factor expression and administration routes on thrombosis development induced by mesenchymal stem/stromal cell infusions: re-evaluating the dogma Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-27 Van T. Hoang, Duc Son Le, Duc M. Hoang, Trang Thi Kieu Phan, Lan Anh Thi Ngo, Trung Kien Nguyen, Viet Anh Bui, Liem Nguyen Thanh
Hyperactive coagulation might cause dangerous complications such as portal vein thrombosis and pulmonary embolism after mesenchymal stem/stromal cell (MSC) therapy. Tissue factor (TF), an initiator of the extrinsic coagulation pathway, has been suggested as a predictor of this process. The expression of TF and other pro- and anticoagulant genes was analyzed in xeno- and serum-free manufactured MSCs
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Oxidized SOD1 accelerates cellular senescence in neural stem cells Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-27 Teng Guan, Ying Guo, Ting Zhou, Qiang Yu, Jingyi Sun, Baoliang Sun, Guohui Zhang, Jiming Kong
Neural stem cells (NSCs), especially human NSCs, undergo cellular senescence characterized by an irreversible proliferation arrest and loss of stemness after prolonged culture. While compelling correlative data have been generated to support the oxidative stress theory as one of the primary determinants of cellular senescence of NSCs, a direct cause-and-effect relationship between the accumulation
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Identifying Hmga2 preserving visual function by promoting a shift of Müller glia cell fate in mice with acute retinal injury Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-27 Zhiyuan Yin, Lingling Ge, Zhe Cha, Hui Gao, Luodan A, Yuxiao Zeng, Xiaona Huang, Xuan Cheng, Kai Yao, Zui Tao, Haiwei Xu
Unlike in lower vertebrates, Müller glia (MG) in adult mammalian retinas lack the ability to reprogram into neurons after retinal injury or degeneration and exhibit reactive gliosis instead. Whether a transition in MG cell fate from gliosis to reprogramming would help preserve photoreceptors is still under exploration. A mouse model of retinitis pigmentosa (RP) was established using MG cell lineage
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Correction: Clinical application of a double-modified sulfated bacterial cellulose scaffold material loaded with FGFR2-modified adipose-derived stem cells in urethral reconstruction Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-27 Zhenpeng Zhu, Jiayu Yang, Xing Ji, Zicheng Wang, Chengxiang Dai, Suke Li, Xuesong Li, Yajie Xie, Yudong Zheng, Jian Lin, Liqun Zhou
Correction to: Stem Cell Research and Therapy (2022) 13:463 https://doi.org/10.1186/s13287-022-03164-9 Following the publication of the original article [1], the authors have identified some errors in the Figs. 2, 4, 5, 7 and Additional file 1: Fig. S1. The correction are as follows: Fig. 2 A Microstructure of BC, MBC, SBC, and SMBC; B surface scan images of BC, SBC, and SMBCFull size image Fig. 4
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Retraction Note: Mesenchymal stem cells ameliorate renal fibrosis by galectin-3/Akt/GSK3β/Snail signaling pathway in adenine-induced nephropathy rat Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-27 Huajun Tang, Peiyue Zhang, Lianlin Zeng, Yu Zhao, Libo Xie, Bo Chen
Retraction Note: Stem Cell Research & Therapy (2021) 12:409 https://doi.org/10.1186/s13287-021-02429-z RETRACTION NOTE. The Editors in Chief have retracted this article after concerns were raised about the data reported. Specifically: In Fig. 7a, there is evidence of a potential splice between lanes 2 and 3 of GAPDH blot. The GAPDH blot of Fig. 11a appears to duplicate the GAPDH blot of Fig. 11c. In
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Retraction Note: MicroRNA-375 released from extracellular vesicles of bone marrow mesenchymal stem cells exerts anti-oncogenic effects against cervical cancer Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-27 Feng Ding, Jinhua Liu, Xiaofei Zhang
Stem Cell Research & Therapy (2020) 11:455 https://doi.org/10.1186/s13287-020-01908-z Retraction note The Editors-in-Chief have retracted this article. After publication, concerns were raised regarding similar features between this article and a number of other articles on non-coding RNAs from different research groups published within a similar time frame [1,2,3,4]. Specifically, the ruler in Fig
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Retraction Note: Secretome of endothelial progenitor cells from stroke patients promotes endothelial barrier tightness and protects against hypoxia-induced vascular leakage Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-27 Rodrigo Azevedo Loiola, Miguel García-Gabilondo, Alba Grayston, Paulina Bugno, Agnieszka Kowalska, Sophie Duban-Deweer, Eleonora Rizzi, Johan Hachani, Yasuteru Sano, Fumitaka Shimizu, Takashi Kanda, Caroline Mysiorek, Maciej Piotr Mazurek, Anna Rosell, Fabien Gosselet
Stem Cell Research & Therapy (2021) 12:552 https://doi.org/10.1186/s13287-021-02608-y The authors have retracted this article. After the publication of this article, the authors carried out a voluntary and exhaustive investigation of the endothelial progenitor cells (EPC) cryovials used in this study. This investigation was performed to verify the authenticity of human cell lines as a quality control
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Model construction and drug therapy of primary ovarian insufficiency by ultrasound-guided injection Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-20 Fangfang Dai, Hua Liu, Juan He, Jinglin Wu, Chaoyan Yuan, Ruiqi Wang, Mengqin Yuan, Dongyong Yang, Zhimin Deng, Linlin Wang, Yanqing Wang, Xiao Yang, Huiling Wang, Wei Hu, Yanxiang Cheng
Clinically, hormone replacement therapy (HRT) is the main treatment for primary ovarian insufficiency (POI). However, HRT may increase the risk of both breast cancer and cardiovascular disease. Exosomes derived from human umbilical cord mesenchymal stem cell (hUC-MSC) have been gradually applied to the therapy of a variety of diseases through inflammation inhibition, immune regulation, and tissue repair
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Kupffer cells abrogate homing and repopulation of allogeneic hepatic progenitors in injured liver site Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-20 Nasir Abbas, Kai You, Anteneh Getachew, Feima Wu, Muzammal Hussain, Xinping Huang, Yan Chen, Tingcai Pan, Yinxiong Li
Allogeneic hepatocyte transplantation is an emerging approach to treat acute liver defects. However, durable engraftment of the transplanted cells remains a daunting task, as they are actively cleared by the recipient’s immune system. Therefore, a detailed understanding of the innate or adaptive immune cells-derived responses against allogeneic transplanted hepatic cells is the key to rationalize cell-based
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Correction: Follistatin-like 1 protects mesenchymal stem cells from hypoxic damage and enhances their therapeutic efficacy in a mouse myocardial infarction model Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-20 Han Shen, Guanghao Cui, Yanqiong Li, Wenxue Ye, Yimin Sun, Zihan Zhang, Jingjing Li, Guiying Xu, Xiansheng Zeng, Yanxia Zhang, Wencheng Zhang, Zan Huang, Weiqian Chen, Zhenya Shen
Correction : Stem Cell Research & Therapy (2019) 10:17 https://doi.org/10.1186/s13287-018-1111-y Following publication of this article [1], the authors found that incorrect representative echocardiographic images were mistakenly included in Fig. 5a (MSCs-mCherry group and MSCs-Fstl1 group) during manuscript preparation, which partially overlapped with representative echocardiographic images in our
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Gingival-derived mesenchymal stem cell therapy regenerated the radiated salivary glands: functional and histological evidence in murine model Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-16 Hagar M. Zayed, Nevine H. Kheir El Din, Ashraf M. Abu-Seida, Asmaa A. Abo Zeid, Ola M. Ezzatt
Radiotherapy in head and neck cancer management causes degeneration of the salivary glands (SG). This study was designed to determine the potential of gingival mesenchymal stem cells (GMSCs) as a cell-based therapy to regenerate irradiated parotid SG tissues and restore their function using a murine model. Cultured isolated cells from gingival tissues of 4 healthy guinea pigs at passage 3 were characterized
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Correction: Mesenchymal stem cells transplantation for perianal fistulas: a systematic review and meta-analysis of clinical trials Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-16 H. Wang, H. Y. Jiang, Y. X. Zhang, H. Y. Jin, B. Y. Fei, J. L. Jiang
Correction: Stem Cell Research & Therapy (2023) 14:103 https://doi.org/10.1186/s13287-023-03331-6 After the publication of this article, the authors noticed an error in the subheading of the Results section. The error was introduced during the revision process when the authors replaced the textual description in the subheading with symbols. The subheading “Meta 1: Efficacy of MSCs for complex perianal
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TC14012 enhances the anti-fibrosis effects of UC-MSCs on the liver by reducing collagen accumulation and ameliorating inflammation Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-16 Fan Ding, Yuting Liu, Jia Li, Xiao Wei, Jiangdong Zhao, Xiaojing Liu, Liqiang Zhang
Mesenchymal stem cells (MSCs) are attracting attention as a promising cell-based therapy for the treatment of liver fibrosis or cirrhosis. However, the strategies and potential mechanisms of MSCs therapy need further investigation. The CXCL12/CXCR4/CXCR7 chemokine axis is well known to regulate cell migration and is involved in the regulation of liver fibrosis. This study aims to treat MSCs with a
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The m6A methylation and expression profiles of mouse neural stem cells after hypoxia/reoxygenation Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-16 Shaoqiong Zhang, Kaile Cui, Yuanyuan Li, Yiting Fan, Dongxu Wang, Xingen Yao, Bo Fang
Ischemia–reperfusion injury to the central nervous system often causes severe complications. The activation of endogenous neural stem cells (NSCs) is considered a promising therapeutic strategy for nerve repair. However, the specific biological processes and molecular mechanisms of NSC activation remain unclear, and the role of N6-methyladenosine (m6A) methylation modification in this process has not
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Editorial Expression of Concern: Intraarterial transplantation of human umbilical cord blood mononuclear cells is more efficacious and safer compared with umbilical cord mesenchymal stromal cells in a rodent stroke model Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-16
Editorial Expression of Concern: Stem Cell Research & Therapy 2014, 5:45 https://doi.org/10.1186/scrt434 The Editors-in-Chief are issuing this editorial expression of concern to alert readers that in Figure 4 there is overlap between the cbMSC and the cmMSC images of the Ipsilateral Striatum. Due to the age of the article the authors are unable to access and provide the original raw data for evaluation
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The application of a 4D-printed chitosan-based stem cell carrier for the repair of corneal alkali burns Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-14 Zibo Wang, Changqing Jiang, Yuqiao Fan, Xiaodan Hao, Yanhan Dong, Xinjia He, Jinning Gao, Yongchun Zhang, Meng Li, Mengyuan Wang, Yiming Liu, Wenhua Xu
Corneal alkali burns can lead to ulceration, perforation, and even corneal blindness due to epithelial defects and extensive cell necrosis, resulting in poor healing outcomes. Previous studies have found that chitosan-based in situ hydrogel loaded with limbal epithelium stem cells (LESCs) has a certain reparative effect on corneal alkali burns. However, the inconsistent pore sizes of the carriers and
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Correction: Effect of once versus twice intracoronary injection of allogeneic-derived mesenchymal stromal cells after acute myocardial infarction: BOOSTER-TAHA7 randomized clinical trial Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-14 Armin Attar, Mohsen Farjoud Kouhanjani, Kamran Hessami, Massoud Vosough, Javad Kojuri, Mani Ramzi, Seyed Ali Hosseini, Marjan Faghih, Ahmad Monabati
Correction: Stem Cell Research & Therapy (2023) 14:264 https://doi.org/10.1186/s13287-023-03495-1 In the original article, the authors identified that at the 3rd page of article in right column in the 12th line they had forgotten to add the name of the cell product used for the trial. This line should be corrected as follows: “On each day of infusion, fresh cGMP-certified clinical-grade hWJ-MSCs (Whartocell®
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Correction: Primary explants of the postnatal thymus allow the expansion of clonogenic thymic epithelial cells that constitute thymospheres Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-14 Juan M. Ocampo-Godinez, Jose L. Gonzalez-Quiroz, Hector Cote-Palafox, Elizabeth George, Jael A. Vergara-Lope Nuñez, Guillermo Villagomez-Olea, Febe C. Vazquez-Vazquez, Edgar O. Lopez-Villegas, Gloria Leon-Avila, Maria L. Dominguez-Lopez, Marco A. Alvarez-Perez
Correction: Stem Cell Research & Therapy (2023) 14:312 https://doi.org/10.1186/s13287-023-03529-8 The original article contains an erroneous attribution of co-author, Gloria Leon-Avila to affiliation #3. Dr Leon-Avila should instead be affiliated to affiliation #2 as shown in this correction article. Authors and Affiliations Laboratorio de Bioingeniería de Tejidos, División de Estudios de Posgrado
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NOD1 deficiency ameliorates the progression of diabetic retinopathy by modulating bone marrow–retina crosstalk Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-09 Jingwen Qiu, Jing Wu, Wenwen Chen, Yu Ruan, Jingning Mao, Shue Li, Xuan Tang, Lei Zhao, Shengbing Li, Ke Li, Dongfang Liu, Yaqian Duan
Nucleotide-binding oligomerization domain-containing protein 1 (NOD1) plays a pivotal role in inducing metabolic inflammation in diabetes. Additionally, the NOD1 ligand disrupts the equilibrium of bone marrow-derived hematopoietic stem/progenitor cells, a process that has immense significance in the development of diabetic retinopathy (DR). We hypothesized that NOD1 depletion impedes the advancement
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Adipose-derived stem cells in immune-related skin disease: a review of current research and underlying mechanisms Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-08 Tianyi Sun, Cheng Zhou, Feng Lu, Ziqing Dong, Jianhua Gao, Bin Li
Adipose-derived stem cells (ASCs) are a critical adult stem cell subpopulation and are widely utilized in the fields of regenerative medicine and stem cell research due to their abundance, ease of harvest, and low immunogenicity. ASCs, which are homologous with skin by nature, can treat immune-related skin diseases by promoting skin regeneration and conferring immunosuppressive effects, with the latter
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The cross talk between type II diabetic microenvironment and the regenerative capacities of human adipose tissue-derived pericytes: a promising cell therapy Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-08 Toka A. Ahmed, Sara M. Ahmed, Hoda Elkhenany, Mohamed A. El-Desouky, Sameh Magdeldin, Aya Osama, Ali Mostafa Anwar, Ihab K. Mohamed, Mohamed Essameldin Abdelgawad, Demiana H. Hanna, Nagwa El-Badri
Pericytes (PCs) are multipotent contractile cells that wrap around the endothelial cells (ECs) to maintain the blood vessel's functionality and integrity. The hyperglycemia associated with Type 2 diabetes mellitus (T2DM) was shown to impair the function of PCs and increase the risk of diabetes complications. In this study, we aimed to investigate the deleterious effect of the diabetic microenvironment
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Grafted human-induced pluripotent stem cells-derived oligodendrocyte progenitor cells combined with human umbilical vein endothelial cells contribute to functional recovery following spinal cord injury Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-07 Qian Li, Sumei Liu, Tianqi Zheng, Mo Li, Boling Qi, Liping Zhou, Bochao Liu, Dan Ma, Chao Zhao, Zhiguo Chen
Spinal cord injury (SCI) is a devastating disease that causes extensive damage to oligodendrocytes and neurons leading to demyelination and axonal degeneration. In this study, we co-transplanted cell grafts containing oligodendrocyte progenitor cells (OPCs) derived from human-induced pluripotent stem cells (iPSCs) combined with human umbilical vein endothelial cells (HUVECs), which were reported to
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Natural compounds and mesenchymal stem cells: implications for inflammatory-impaired tissue regeneration Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-07 Wen Li, Zichao Xiang, Wenjing Yu, Xiaobin Huang, Qian Jiang, Arwa Abumansour, Ying Yang, Chider Chen
Inflammation is a common and important pathological process occurring in any part of the body and relating to a variety of diseases. Effective tissue repair is critical for the survival of impaired organisms. Considering the side effects of the currently used anti-inflammatory medications, new therapeutic agents are urgently needed for the improvement of regenerative capacities of inflammatory-impaired
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Osteogenic human MSC-derived extracellular vesicles regulate MSC activity and osteogenic differentiation and promote bone regeneration in a rat calvarial defect model Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-07 Niyaz Al-Sharabi, Samih Mohamed-Ahmed, Siddharth Shanbhag, Carina Kampleitner, Rammah Elnour, Shuntaro Yamada, Neha Rana, Even Birkeland, Stefan Tangl, Reinhard Gruber, Kamal Mustafa
There is growing evidence that extracellular vesicles (EVs) play a crucial role in the paracrine mechanisms of transplanted human mesenchymal stem cells (hMSCs). Little is known, however, about the influence of microenvironmental stimuli on the osteogenic effects of EVs. This study aimed to investigate the properties and functions of EVs derived from undifferentiated hMSC (Naïve-EVs) and hMSC during
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The nuclei of human adult stem cells can move within the cell and generate cellular protrusions to contact other cells Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-07 Carlos Bueno, David García-Bernal, Salvador Martínez, Miguel Blanquer, José M. Moraleda
The neuronal transdifferentiation of adult bone marrow cells (BMCs) is still considered an artifact based on an alternative explanation of experimental results supporting this phenomenon obtained over decades. However, recent studies have shown that following neural induction, BMCs enter an intermediate cellular state before adopting neural-like morphologies by active neurite extension and that binucleated
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HAND factors regulate cardiac lineage commitment and differentiation from human pluripotent stem cells Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-05 Huixin Guo, Chengwen Hang, Bowen Lin, Zheyi Lin, Hui Xiong, Mingshuai Zhang, Renhong Lu, Junyang Liu, Dan Shi, Duanyang Xie, Yi Liu, Dandan Liang, Jian Yang, Yi-Han Chen
Transcription factors HAND1 and HAND2 (HAND1/2) play significant roles in cardiac organogenesis. Abnormal expression and deficiency of HAND1/2 result in severe cardiac defects. However, the function and mechanism of HAND1/2 in regulating human early cardiac lineage commitment and differentiation are still unclear. With NKX2.5eGFP H9 human embryonic stem cells (hESCs), we established single and double
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Mesenchymal stem cell-derived exosomes: a promising alternative in the therapy of preeclampsia Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-05 Haoran Shi, Zejun Yang, Jianjian Cui, Hui Tao, Ruilin Ma, Yin Zhao
Preeclampsia (PE) is a common morbid complication during pregnancy, affecting 2%-8% of pregnancies globally and posing serous risks to the health of both mother and fetus. Currently, the only effective treatment for PE is timely termination of pregnancy, which comes with increased perinatal risks. However, there is no effective way to delay pathological progress and improve maternal and fetal outcomes
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Efficacy of the immediate adipose-derived stromal vascular fraction autograft on functional sensorimotor recovery after spinal cord contusion in rats Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-02 Céline Ertlen, Mostafa Seblani, Maxime Bonnet, Jean-Michel Brezun, Thelma Coyle, Florence Sabatier, Stéphane Fuentes, Patrick Decherchi, Nicolas Serratrice, Tanguy Marqueste
Spinal cord injuries (SCI) lead to functional alteration with important consequences such as motor and sensory disorders. The repair strategies developed to date remain ineffective. The adipose tissue-derived stromal vascular fraction (SVF) is composed of a cocktail of cells with trophic, pro-angiogenic and immunomodulatory effects. Numerous therapeutic benefits were shown for tissue reconstitution
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Efficacy and safety of stem cell therapy for Crohn’s disease: a meta-analysis of randomized controlled trials Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-02 Yunfeng Qiu, Changfeng Li, Shihou Sheng
Small-scale clinical trials have provided evidence suggesting the effectiveness of stem-cell therapy (SCT) for patients diagnosed with Crohn's disease (CD). The objective of the research was to systematically assess the effectiveness and safety of SCT for individuals diagnosed with CD through a comprehensive review and meta-analysis. A search was conducted in Medline (PubMed), CENTER (Cochrane Library)
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The comparison of adipose-derived stromal cells (ADSCs) delivery method in a murine model of hindlimb ischemia Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-02-02 Ewelina Pilny, Justyna Czapla, Alina Drzyzga, Ryszard Smolarczyk, Sybilla Matuszczak, Magdalena Jarosz-Biej, Łukasz Krakowczyk, Tomasz Cichoń
Adipose-derived stromal cells (ADSCs) demonstrate ability to promote tissue healing and down-regulate excessive inflammation. ADSCs have been used to treat critical limb ischemia in preclinical and clinical trials, but still, there is little known about their optimal delivery strategy. To date, no direct analysis of different methods of ADSCs delivery has been performed in the hindlimb ischemia model
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Culture and expansion of murine proximal airway basal stem cells Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-01-30 Meirong Wu, Xiaojing Zhang, Yanjuan Tu, Wenzhao Cheng, Yiming Zeng
The stem cell characteristic makes basal cells desirable for ex vivo modeling of airway diseases. However, to date, approaches allowing them extensively in vitro serial expansion and maintaining bona fide stem cell property are still awaiting to be established. This study aims to develop a feeder-free culture system of mouse airway basal stem cells (ABSCs) that sustain their stem cell potential in
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Glutathione supplementation improves fat graft survival by inhibiting ferroptosis via the SLC7A11/GPX4 axis Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-01-30 Zehua Li, Jinqiang Lu, Zhiqin Dong, Jiaji Liang, Shenghong Li, Wenwen Han, Taixing Cui, Hongwei Liu
Autologous fat grafting is hampered by unpredictable graft survival, which is potentially regulated by ferroptosis. Glutathione (GSH), a powerful antioxidant used in tissue preservation, has ferroptosis-regulating activity; however, its effects on fat grafts are unclear. This study investigated the effects and mechanisms of GSH in fat graft survival. Human lipoaspirates were transplanted subcutaneously
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Allogenic hematopoietic stem cell transplantation outcomes of patients aged ≥ 55 years with acute myeloid leukemia or myelodysplastic syndromes in China: a retrospective study Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-01-29 Lu Gao, Li Yang, Shiyuan Zhou, Wenjuan Zhu, Yue Han, Suning Chen, Shengli Xue, Ying Wang, Huiying Qiu, Depei Wu, Xiaojin Wu
Elderly patients with acute myeloid leukemia or myelodysplastic syndromes (AML/MDS) have historically had poor prognoses. However, there has been a recent increase in the use of allogenic hematopoietic stem cell transplantation (allo-HSCT) are in this patient population. Nevertheless, the optimal choice of donor type for the patients remains an unmet need. Limited data exist on the use of allo-HSCT
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Current status of stem cell therapy for type 1 diabetes: a critique and a prospective consideration Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-01-29 Mohamed A. Ghoneim, Mahmoud M. Gabr, Sawsan M. El-Halawani, Ayman F. Refaie
Over the past decade, there had been progress in the development of cell therapy for insulin-dependent diabetes. Nevertheless, important hurdles that need to be overcome still remain. Protocols for the differentiation of pluripotent stem cells into pancreatic progenitors or fully differentiated β-cells have been developed. The resulting insulin-producing cells can control chemically induced diabetes
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Dysregulated lncRNAs regulate human umbilical cord mesenchymal stem cell differentiation into insulin-producing cells by forming a regulatory network with mRNAs Stem Cell Res. Ther. (IF 7.5) Pub Date : 2024-01-25 Tianqin Xie, Qiming Huang, Qiulan Huang, Yanting Huang, Shuang Liu, Haixia Zeng, Jianping Liu
In recent years, cell therapy has emerged as a new research direction in the treatment of diabetes. However, the underlying molecular mechanisms of mesenchymal stem cell (MSC) differentiation necessary to form such treatment have not been clarified. In this study, human umbilical cord mesenchymal stem cells (HUC-MSCs) isolated from newborns were progressively induced into insulin-producing cells (IPCs)