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Cell cycle perturbation uncouples mitotic progression and invasive behavior in a post-mitotic cell Differentiation (IF 2.9) Pub Date : 2024-03-11 Michael A.Q. Martinez, Chris Z. Zhao, Frances E.Q. Moore, Callista Yee, Wan Zhang, Kang Shen, Benjamin L. Martin, David Q. Matus
The acquisition of the post-mitotic state is crucial for the execution of many terminally differentiated cell behaviors during organismal development. However, the mechanisms that maintain the post-mitotic state in this context remain poorly understood. To gain insight into these mechanisms, we used the genetically and visually accessible model of anchor cell (AC) invasion into the vulval epithelium
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Oxidative-stress induced Bmp2-Smad1/5/8 signaling dependent differentiation of early cardiomyocytes from embryonic and adult epicardial cells Differentiation (IF 2.9) Pub Date : 2024-03-02 Madhurima Ghosh, Riffat Khanam, Arunima Sengupta, Santanu Chakraborty
Heart failure has become a major life-threatening cause affecting millions globally, characterized by the permanent loss of adult functional cardiomyocytes leading to fibrosis which ultimately deprives the heart of its functional efficacy. Here we investigated the reparative property of embryonic and adult epicardial cells towards cardiomyocyte differentiation under oxidative stress-induced conditions
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Type 1 collagen: Synthesis, structure and key functions in bone mineralization Differentiation (IF 2.9) Pub Date : 2024-02-28 Vimalraj Selvaraj, Saravanan Sekaran, Anuradha Dhanasekaran, Sudha Warrier
Collagen is a highly abundant protein in the extracellular matrix of humans and mammals, and it plays a critical role in maintaining the body's structural integrity. Type I collagen is the most prevalent collagen type and is essential for the structural integrity of various tissues. It is present in nearly all connective tissues and is the main constituent of the interstitial matrix. Mutations that
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The road to gene manipulation in the mouse jean brachet memorial lecture of the international society of differentiation delivered June 21, 2023 at cold spring harbor laboratory Differentiation (IF 2.9) Pub Date : 2024-01-29 Virginia Papaioannou Emerita
Genetic manipulation in mammals has progressed rapidly in the past decade with the advent of CRISPR-Cas gene editing tools, promising profound impacts on the understanding of human development, health and disease. However, many years of research in divergent fields of experimental embryology, genetics, reproduction, molecular biology and transgenic technology laid the groundwork and have played critical
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Regulation of adipogenesis by histone methyltransferases Differentiation (IF 2.9) Pub Date : 2024-01-14 Yuanxiang Zhao, Zachary Skovgaard, Qinyi Wang
Epigenetic regulation is a critical component of lineage determination. Adipogenesis is the process through which uncommitted stem cells or adipogenic precursor cells differentiate into adipocytes, the most abundant cell type of the adipose tissue. Studies examining chromatin modification during adipogenesis have provided further understanding of the molecular blueprint that controls the onset of adipogenic
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BBS genes are involved in accelerated proliferation and early differentiation of BBS-related tissues Differentiation (IF 2.9) Pub Date : 2024-01-06 Avital Horwitz, Noa Levi-Carmel, Olga Shnaider, Ruth Birk
Bardet-Biedl syndrome (BBS) is an inherited disorder primarily ciliopathy with pleiotropic multi-systemic phenotypic involvement, including adipose, nerve, retinal, kidney, Etc. Consequently, it is characterized by obesity, cognitive impairment and retinal, kidney and cutaneous abnormalities. Initial studies, including ours have shown that BBS genes play a role in the early developmental stages of
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Differentiation of Uc-MSCs into insulin secreting islet-like clusters by trypsin through TGF-beta signaling pathway Differentiation (IF 2.9) Pub Date : 2023-12-16 Feirong Huang, Jiashuang Lai, Lixia Qian, Wanjin Hong, Liang-cheng Li
Differentiation of human umbilical cord mesenchymal stem cells (Uc-MSCs) into islet-like clusters which are capable of synthesizing and secreting insulin can potentially serve as donors for islet transplantation in the patient deficiency in islet β cell function both in type 1 or type 2 diabetic patients. Therefore, we developed an easy and higher efficacy approach by trypsinazing the Uc-MSCs and followed
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3D hepatic organoid production from human pluripotent stem cells Differentiation (IF 2.9) Pub Date : 2023-12-12 Zhe-Long Jin, KangHe Xu, Jonghun Kim, Hao Guo, Xuerui Yao, Yong-Nan Xu, Ying-Hua Li, DongHee Ryu, Kee-Pyo Kim, Kwonho Hong, Yong-June Kim, Lin Wang, Qilong Cao, Kyun-Hwan Kim, Nam-Hyung Kim, Dong Wook Han
Hepatic organoids might provide a golden opportunity for realizing precision medicine in various hepatic diseases. Previously described hepatic organoid protocols from pluripotent stem cells rely on complicated multiple differentiation steps consisting of both 2D and 3D differentiation procedures. Therefore, the spontaneous formation of hepatic organoids from 2D monolayer culture is associated with
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Expression patterns of CYP26A1, FGF8, CDKN1A, and NPVF in the developing rhesus monkey retina Differentiation (IF 2.9) Pub Date : 2023-12-14 Miranda R. Krueger, Elizabeth Fishman-Williams, Sergi Simó, Alice F. Tarantal, Anna La Torre
The fovea centralis (fovea) is a specialized region of the primate retina that plays crucial roles in high-resolution visual acuity and color perception. The fovea is characterized by a high density of cone photoreceptors and no rods, and unique anatomical properties that contribute to its remarkable visual capabilities. Early histological analyses identified some of the key events that contribute
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Primer special issue on FGFs: FGF12 Differentiation (IF 2.9) Pub Date : 2023-11-25 Martyna Biadun, Radoslaw Karelus, Daniel Krowarsch, Lukasz Opalinski, Malgorzata Zakrzewska
Fibroblast growth factor 12 (FGF12) belongs to the fibroblast growth factor homologous factors (FHF) subfamily, which is also known as the FGF11 subfamily. The human FGF12 gene is located on chromosome 3 and consists of four introns and five coding exons. Their alternative splicing results in two FGF12 isoforms – the shorter ‘b’ isoform and the longer ’a’ isoform. Structurally, the core domain of FGF12
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Fibroblast growth factor 10 Differentiation (IF 2.9) Pub Date : 2023-11-25 Francesca Rochais, Robert G. Kelly
Fibroblast growth factor 10 (FGF10) is a major morphoregulatory factor that plays essential signaling roles during vertebrate multiorgan development and homeostasis. Fgf10 is predominantly expressed in mesenchymal cells and signals though FGFR2b in adjacent epithelia to regulate branching morphogenesis, stem cell fate, tissue differentiation and proliferation, in addition to autocrine roles. Genetic
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Ovotesticular cords and ovotesticular follicles: New markers in a model of human mixed ovotestis Differentiation (IF 2.9) Pub Date : 2023-11-19 Laurence Baskin, Mei Cao, Sena Askel, Yi Li, Gerald Cunha
Abstract not available
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Network analysis of transcriptomic data uncovers molecular signatures and the interplay of mRNAs, lncRNAs, and miRNAs in human embryonic stem cells Differentiation (IF 2.9) Pub Date : 2023-11-14 Arindam Ghosh, Anup Som
Growing evidence has shown that besides the protein coding genes, the non-coding elements of the genome are indispensable for maintaining the property of self-renewal in human embryonic stem cells and in cell fate determination. However, the regulatory mechanisms and the landscape of interactions between the coding and non-coding elements is poorly understood. In this work, we used weighted gene co-expression
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FGF18 primer Differentiation (IF 2.9) Pub Date : 2023-11-07 Michael G.E. Goldschagg, Dorit Hockman
FGF18 was discovered in 1998. It is a pleiotropic growth factor that stimulates major signalling pathways involved in cell proliferation and growth, and is involved in the development and homeostasis of many tissues such as bone, lung, and central nervous system. The gene consists of five exons that code for a 207 amino acid glycosylated protein. FGF18 is widely expressed in developing and adult chickens
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FGF5 Differentiation (IF 2.9) Pub Date : 2023-11-07 Evelyn A. Carrion, Malcolm M. Moses, Richard R. Behringer
FGF5 functions as a negative regulator of the hair cycle in mammals. It is expressed in the outer root sheath of hair follicles during the late anagen phase of the hair cycle. It functions as a signaling molecule, mediating the transition of the anagen growth phase to catagen regression phase of hair cycle. Spontaneous and engineered FGF5 mutations in mammalian animal models result in long hair phenotypes
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FGF20 primer Differentiation (IF 2.9) Pub Date : 2023-11-07 Justine D. Van Greenen, Dorit Hockman
Fibroblast growth factor 20 (FGF20) is a neurotrophic factor and a member of the FGF9 subfamily. It was first identified in Xenopus embryos and was isolated shortly thereafter from the adult rat brain. Its receptors include FGFR4, FGFR3b, FGFR2b and the FGFRc splice forms. In adults it is highly expressed in the brain, while it is expressed in a variety of regions during embryonic development, including
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AKT from dental epithelium to papilla promotes odontoblast differentiation Differentiation (IF 2.9) Pub Date : 2023-10-21 Jiangyi Wang, Xiaoyu Lin, Zongshan Shen, Guoqing Li, Lei Hu, Qiong Li, Yang Li, Jinsong Wang, Chunmei Zhang, Songlin Wang, Xiaoshan Wu
Epithelial–mesenchymal interactions occur during tooth development. The dental epithelium (DE) is regarded as the signal center that regulates tooth morphology. However, the mechanism by which DE regulates the differentiation of mesenchyme-derived dental papilla (DP) into odontoblasts remains unclear. Using miniature pigs as a model, we analyzed the expression profiles of the DE and DP during odontoblast
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Fibroblast growth factor 2 Differentiation (IF 2.9) Pub Date : 2023-10-12 Audrey Nickle, Sebastian Ko, Amy E. Merrill
Fibroblast Growth Factor 2 (FGF2), also known as basic fibroblast growth factor, is a potent stimulator of growth and differentiation in multiple tissues. Its discovery traces back over 50 years ago when it was first isolated from bovine pituitary extracts due to its ability to stimulate fibroblast proliferation. Subsequent studies investigating the genomic structure of FGF2 identified multiple protein
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Type H vessels in osteogenesis, homeostasis, and related disorders Differentiation (IF 2.9) Pub Date : 2023-09-23 Xiaoru Qin, Yue Xi, Qifeng Jiang, Chaozhen Chen, Guoli Yang
The vascular system plays a crucial role in bone tissue. Angiogenic and osteogenic processes are coupled through a spatial-temporal connection. Recent studies have identified three types of capillaries in the skeletal system. Compared with type L and E vessels, type H vessels express high levels of CD31 and endomucin, and function to couple angiogenesis and osteogenesis. Endothelial cells in type H
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Effects of a Sertoli cell-specific knockout of Connexin43 on maturation and proliferation of postnatal Sertoli cells Differentiation (IF 2.9) Pub Date : 2023-09-25 Hanna Hüneke, Marion Langeheine, Kristina Rode, Klaus Jung, Adrian Pilatz, Daniela Fietz, Sabine Kliesch, Ralph Brehm
Adult male Sertoli cell-specific Connexin43 knockout mice (SCCx43KO) exhibit higher Sertoli cell (SC) numbers per seminiferous tubule compared to their wild type (WT) littermates. Thus, deletion of this testicular gap junction protein seems to affect the proliferative potential and differentiation of “younger” SC. Although SC have so far mostly been characterised as postmitotic cells that cease to
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The fundamentals of fibroblast growth factor 9 Differentiation (IF 2.9) Pub Date : 2023-09-22 Hao Yin, Sabrina C.R. Staples, J. Geoffrey Pickering
Fibroblast growth factor 9 (FGF9) was first identified during a screen for factors acting on cells of the central nervous system (CNS). Research over the subsequent two decades has revealed this protein to be a critically important and elegantly regulated growth factor. A hallmark control feature is reciprocal compartmentalization, particularly during development, with epithelium as a dominant source
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Primer on FGF3 Differentiation (IF 2.9) Pub Date : 2023-09-16 Carmel Hutchings, Dalit Sela-Donenfeld
Though initially discovered as a proto-oncogene in virally induced mouse mammary tumors, FGF3 is primarily active in prenatal stages, where it is found at various sites at specific times. FGF3 is crucial during development, as its roles include tail formation, inner ear development and hindbrain induction and patterning. FGF3 expression and function are highly conserved in vertebrates, while it also
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Epithelial-fibroblast interactions in IPF: Lessons from in vitro co-culture studies Differentiation (IF 2.9) Pub Date : 2023-09-12 J. Brussow, K. Feng, F. Thiam, S. Phogat, E.T. Osei
Idiopathic pulmonary fibrosis (IPF) is a progressive interstitial disease that is characterized by increased cellular proliferation and differentiation together with excessive extracellular matrix (ECM) deposition leading to buildup of scar tissue (fibrosis) and remodeling in the lungs. The activated and differentiated (myo)fibroblasts are one of the main sources of tissue remodeling in IPF and a crucial
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The role of non-coding RNAs in diabetes-induced osteoporosis Differentiation (IF 2.9) Pub Date : 2023-08-22 Er-li Wu, Ming Cheng, Xin-jing Zhang, Tian-gang Wu, Lei Zhang
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Ectopic expression of HNF4α in Het1A cells induces an invasive phenotype Differentiation (IF 2.9) Pub Date : 2023-08-23 Carmen Grimaldos Rodriguez, Ella F. Rimmer, Benjamin Colleypriest, David Tosh, Jonathan M.W. Slack, Ute Jungwirth
Barrett's oesophagus (BO) is a pathological condition in which the squamous epithelium of the distal oesophagus is replaced by an intestinal-like columnar epithelium originating from the gastric cardia. Several somatic mutations contribute to the intestinal-like metaplasia. Once these have occurred in a single cell, it will be unable to expand further unless the altered cell can colonise the surrounding
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Inductive effect of SORT1 on odontoblastic differentiation of human dental pulp-derived stem cells Differentiation (IF 2.9) Pub Date : 2023-08-09 Daisuke Omagari, Taku Toriumi, Hiromasa Tsuda, Manabu Hayatsu, Keisuke Watanabe, Yusuke Mizutami, Masaki Honda, Yoshikazu Mikami
This study investigated the expression of sortilin 1 (SORT1) in cultured human dental pulp-derived stem cells (hDPSCs) and its role in their odontoblastic differentiation. Permanent teeth were extracted from five patients, and the dental pulp was harvested for explant culture. Fluorescence-activated cell sorting was used to analyze the outgrowth of adherent cells and cells that had migrated from the
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Intravitreal injection of FGF and TGF-β inhibitors disrupts cranial cartilage development Differentiation (IF 2.9) Pub Date : 2023-07-18 Nicholas W. Zinck, Shea J.L. McInnis, Tamara A. Franz-Odendaal
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Ex vivo adult stem cell characterization from multiple muscles in ambulatory children with cerebral palsy during early development of contractures Differentiation (IF 2.9) Pub Date : 2023-07-12
Cerebral palsy (CP) is one of the most common conditions leading to lifelong childhood physical disability. Literature reported previously altered muscle properties such as lower number of satellite cells (SCs), with altered fusion capacity. However, these observations highly vary among studies, possibly due to heterogeneity in patient population, lack of appropriate control data, methodology and different
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Sonic hedgehog signaling in craniofacial development Differentiation (IF 2.9) Pub Date : 2023-07-13 Jingyue Xu, Paul P.R. Iyyanar, Yu Lan, Rulang Jiang
Mutations in SHH and several other genes encoding components of the Hedgehog signaling pathway have been associated with holoprosencephaly syndromes, with craniofacial anomalies ranging in severity from cyclopia to facial cleft to midfacial and mandibular hypoplasia. Studies in animal models have revealed that SHH signaling plays crucial roles at multiple stages of craniofacial morphogenesis, from
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Stage-specific requirement for m6A RNA methylation during cardiac differentiation of pluripotent stem cells Differentiation (IF 2.9) Pub Date : 2023-07-13 Shuai Dong, Yuetong Sun, Chang Liu, Yanli Li, Shanshan Yu, Qi Zhang, Yan Xu
Precise spatiotemporal control of gene expression patterns is critical for normal development. Pluripotent stem cells, including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), with the ability of unlimited self-renewal and differentiation into any cell type, provide a unique tool for understanding the underlying mechanism of development and disease in a dish. N6-methyl-adenosine
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Description of trunk neural crest migration and peripheral nervous system formation in the Egyptian cobra Naja haje haje Differentiation (IF 2.9) Pub Date : 2023-07-05 Eraqi R. Khannoon, Christian Alvarado, Rafael Poveda, Maria Elena de Bellard
The neural crest is a stem cell population that forms in the neurectoderm of all vertebrates and gives rise to a diverse set of cells such as sensory neurons, Schwann cells and melanocytes. Neural crest development in snakes is still poorly understood. From the point of view of evolutionary and comparative anatomy is an interesting topic given the unique anatomy of snakes. The aim of the study was
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Ocular Development: A view from the front to the back of the eye Differentiation (IF 2.9) Pub Date : 2023-06-29 Anna La Torre, Peter Lwigale
Abstract not available
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The Rap1 small GTPase affects cell fate or survival and morphogenetic patterning during Drosophila melanogaster eye development Differentiation (IF 2.9) Pub Date : 2023-06-27 Philip P. Yost, Abdul Al-Nouman, Jennifer Curtiss
The Drosophila melanogaster eye has been instrumental for determining both how cells communicate with one another to determine cell fate, as well as cell morphogenesis and patterning. Here, we describe the effects of the small GTPase Rap1 on the development of multiple cell types in the D. melanogaster eye. Although Rap1 has previously been linked to RTK-Ras-MAPK signaling in eye development, we demonstrate
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Osteogenic microenvironment affects palatal development through glycolysis Differentiation (IF 2.9) Pub Date : 2023-05-25 Xia Peng, Jing Chen, Yijia Wang, Xiaotong Wang, Xige Zhao, Xiaoyu Zheng, Zhiwei Wang, Dong Yuan, Juan Du
Palate development involves various events, including proliferation, osteogenic differentiation, and epithelial-mesenchymal transition. Disruption of these processes can result in the cleft palate (CP). Mouse embryonic palatal mesenchyme (MEPM) cells are commonly used to explore the mechanism of palatal development and CP. However, the role of the microenvironment in the biological properties of MEPM
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Disruption of hedgehog signaling leads to hyoid bone dysplasia during embryogenesis Differentiation (IF 2.9) Pub Date : 2023-05-08 Yan Guo, Xingyu Chen, Yongzhen Lai, Meng Lu, Chengyong Wang, Yun Shi, Chengyan Ren, Weihui Chen
The development of the hyoid bone is a complex process that involves the coordination of multiple signaling pathways. Previous studies have demonstrated that disruption of the hedgehog pathway in mice results in a series of structural malformations. However, the specific role and critical period of the hedgehog pathway in the early development of the hyoid bone have not been thoroughly characterized
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Abnormal chondrocyte development in a zebrafish model of cblC syndrome restored by an MMACHC cobalamin binding mutant Differentiation (IF 2.9) Pub Date : 2023-05-05 David Paz, Briana E. Pinales, Barbara S. Castellanos, Isaiah Perez, Claudia B. Gil, Lourdes Jimenez Madrigal, Nayeli G. Reyes-Nava, Victoria L. Castro, Jennifer L. Sloan, Anita M. Quintana
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Meibomian gland development: Where, when and how? Differentiation (IF 2.9) Pub Date : 2023-05-05 Sudhir Verma, Isabel Y. Moreno, Morgan E. Trapp, Luis Ramirez, Tarsis F. Gesteira, Vivien J. Coulson-Thomas
The Meibomian gland (MG) is an indispensable adnexal structure of eye that produces meibum, an important defensive component for maintaining ocular homeostasis. Normal development and maintenance of the MGs is required for ocular health since atrophic MGs and disturbances in composition and/or secretion of meibum result in major ocular pathologies, collectively termed as Meibomian gland dysfunction
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Sf3b4 regulates chromatin remodeler splicing and Hox expression Differentiation (IF 2.9) Pub Date : 2023-05-04 Shruti Kumar, Sabrina Shameen Alam, Eric Bareke, Marie-Claude Beauchamp, Yanchen Dong, Wesley Chan, Jacek Majewski, Loydie A. Jerome-Majewska
SF3B proteins form a heptameric complex in the U2 small nuclear ribonucleoprotein, essential for pre-mRNA splicing. Heterozygous pathogenic variants in human SF3B4 are associated with head, face, limb, and vertebrae defects. Using the CRISPR/Cas9 system, we generated mice with constitutive heterozygous deletion of Sf3b4 and showed that mutant embryos have abnormal vertebral development. Vertebrae abnormalities
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Ordered deployment of distinct ciliary beating machines in growing axonemes of vertebrate multiciliated cells Differentiation (IF 2.9) Pub Date : 2023-04-13 Chanjae Lee, Yun Ma, Fan Tu, John B. Wallingford
The beating of motile cilia requires the coordinated action of diverse machineries that include not only the axonemal dynein arms, but also the central apparatus, the radial spokes, and the microtubule inner proteins. These machines exhibit complex radial and proximodistal patterns in mature axonemes, but little is known about the interplay between them during motile ciliogenesis. Here, we describe
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Epigenetic regulation in the commitment of progenitor cells during retinal development and regeneration Differentiation (IF 2.9) Pub Date : 2023-04-12 Wenjie Yin, Xiying Mao, Miao Xu, Mingkang Chen, Mengting Xue, Na Su, Songtao Yuan, Qinghuai Liu
Retinal development is initiated by multipotent retinal progenitor cells, which undergo several rounds of cell divisions and subsequently terminal differentiation. Retinal regeneration is usually considered as the recapitulation of retinal development, which share common mechanisms underlying the cell cycle re-entry of adult retinal stem cells and the differentiation of retinal neurons. However, how
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Spheroid formation and luteinization of granulosa cells of felids in a long-term 3D culture Differentiation (IF 2.9) Pub Date : 2023-04-06 Michał M. Hryciuk, Filip Schröter, Luise Hennicke, Beate C. Braun
In the present study, granulosa cells (GCs) from domestic cats and Persian leopard were cultured and characterized from selected days. The culture period was divided into two phases: maintenance, which lasted for 7 days, and luteinization, which followed for up to 11 days. Luteinization was performed on ultra-low attachment plates, supporting the formation of spheroids in a medium supplemented with
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Reprogramming of trunk neural crest to a cranial crest-like identity alters their transcriptome and developmental potential Differentiation (IF 2.9) Pub Date : 2023-04-05 Sierra S. Marable, Marianne E. Bronner
Neural crest cells along the body axis of avian embryos differ in their developmental potential, such that the cranial neural crest forms cartilage and bone whereas the trunk neural crest is unable to do so. Previous studies have identified a cranial crest-specific subcircuit that can imbue the trunk neural crest with the ability to form cartilage after grafting to the head. Here, we examine transcriptional
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Characterizing the lens regeneration process in Pleurodeles waltl Differentiation (IF 2.9) Pub Date : 2023-03-11 Georgios Tsissios, Gabriella Theodoroudis-Rapp, Weihao Chen, Anthony Sallese, Byran Smucker, Lake Ernst, Junfan Chen, Yiqi Xu, Sophia Ratvasky, Hui Wang, Katia Del Rio-Tsonis
Background Aging and regeneration are heavily linked processes. While it is generally accepted that regenerative capacity declines with age, some vertebrates, such as newts, can bypass the deleterious effects of aging and successfully regenerate a lens throughout their lifetime. Results Here, we used Optical Coherence Tomography (OCT) to monitor the lens regeneration process of larvae, juvenile, and
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Corneal epithelial development and homeostasis Differentiation (IF 2.9) Pub Date : 2023-03-01 Shivalingappa K. Swamynathan, Sudha Swamynathan
The corneal epithelium (CE), the most anterior cellular structure of the eye, is a self-renewing stratified squamous tissue that protects the rest of the eye from external elements. Each cell in this exquisite three-dimensional structure needs to have proper polarity and positional awareness for the CE to serve as a transparent, refractive, and protective tissue. Recent studies have begun to elucidate
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Androgenic induction of penile features in postnatal female mouse external genitalia from birth to adulthood: Is the female sexual phenotype ever irreversibly determined? Differentiation (IF 2.9) Pub Date : 2023-02-10 Gerald R. Cunha, Mei Cao, Amber Derpinghaus, Laurence S. Baskin
Female mice were treated for 35 days from birth to 60 days postnatal (P0, [birth], P5, P10, P20 and adult [∼P60]) with dihydrotestosterone (DHT). Such treatment elicited profound masculinization the female external genitalia and development of penile features (penile spines, male urogenital mating protuberance (MUMP) cartilage, corpus cavernosum glandis, corporal body, MUMP-corpora cavernosa, a large
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Wiring the ocular surface: A focus on the comparative anatomy and molecular regulation of sensory innervation of the cornea Differentiation (IF 2.9) Pub Date : 2023-01-28 Tyler Schwend
The cornea is richly innervated with sensory nerves that function to detect and clear harmful debris from the surface of the eye, promote growth and survival of the corneal epithelium and hasten wound healing following ocular disease or trauma. Given their importance to eye health, the neuroanatomy of the cornea has for many years been a source of intense investigation. Resultantly, complete nerve
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T, NK, then macrophages: Recent advances and challenges in adaptive immunotherapy from human pluripotent stem cells Differentiation (IF 2.9) Pub Date : 2023-01-18 Su Hang, Nan Wang, Ryohichi Sugimura
Adaptive cellular immunotherapy, especially chimeric antigen receptor-T (CAR-T) cell therapy, has advanced the treatment of hematological malignancy. However, major limitations still remain in the source of cells comes from the patients themselves. The use of human pluripotent stem cells to differentiate into immune cells, such as T cells, NK cells, and macrophages, then arm with chimeric antigen receptor
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Tuberin levels during cellular differentiation in brain development Differentiation (IF 2.9) Pub Date : 2022-12-24 Elizabeth Fidalgo da Silva, Bashaer Abu Khatir, Christopher Drouillard, Isabelle Hinch, Gordon Omar Davis, Mariam Sameem, Rutu Patel, Jackie Fong, Dorota Lubanska, Lisa A. Porter
Tuberin is a member of a large protein complex, Tuberous Sclerosis Complex (TSC), and acts as a sensor for nutrient status regulating protein synthesis and cell cycle progression. Mutations in the Tuberin gene, TSC2, permits the formation of tumors that can lead to developmental defects in many organ systems, including the central nervous system. Tuberin is expressed in the brain throughout development
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The International Society of Differentiation: Past, present, and future Differentiation (IF 2.9) Pub Date : 2022-12-17 Richard R. Behringer, Robert G. McKinnell, Dimitri Viza, Alan O. Perantoni, Elizabeth J. Robertson, Sally L. Dunwoodie, Mark Lewandoski
The International Society of Differentiation was born from the First International Conference on Cell Differentiation conceived by D.V. and held in Nice, France in 1971. The conference also resulted in the creation of the journal of the Society named Differentiation. The Society advocates for the field of differentiation through the journal Differentiation, organizing and supporting international scientific
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Retinoic acid signaling in mouse retina endothelial cells is required for early angiogenic growth Differentiation (IF 2.9) Pub Date : 2022-12-14 Christina N. Como, Cesar Cervantes, Brad Pawlikowski, Julie Siegenthaler
The development of the retinal vasculature is essential to maintain health of the tissue, but the developmental mechanisms are not completely understood. The aim of this study was to investigate the cell-autonomous role of retinoic acid signaling in endothelial cells during retina vascular development. Using a temporal and cell-specific mouse model to disrupt retinoic acid signaling in endothelial
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Zebrafish anterior segment mesenchyme progenitors are defined by function of tfap2a but not sox10 Differentiation (IF 2.9) Pub Date : 2022-12-09 Oliver Vöcking, K. Van Der Meulen, M.K. Patel, J.K. Famulski
The anterior segment is a critical component of the visual system. Developing independent of the retina, the AS relies partially on cranial neural crest cells (cNCC) as its earliest progenitors. The cNCCs are thought to first adopt a periocular mesenchyme (POM) fate and subsequently target to the AS upon formation of the rudimentary retina. AS targeted POM is termed anterior segment mesenchyme (ASM)
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Fgf8 promotes survival of nephron progenitors by regulating BAX/BAK-mediated apoptosis Differentiation (IF 2.9) Pub Date : 2022-12-06 Matthew J. Anderson, Salvia Misaghian, Nirmala Sharma, Alan O. Perantoni, Mark Lewandoski
Fibroblast growth factors (Fgfs) have long been implicated in processes critical to embryonic development, such as cell survival, migration, and differentiation. Several mouse models of organ development ascribe a prosurvival requirement specifically to FGF8. Here, we explore the potential role of prosurvival FGF8 signaling in kidney development. We have previously demonstrated that conditional deletion
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Facile methods for reusing laboratory plastic in developmental biology experiments Differentiation (IF 2.9) Pub Date : 2022-11-21 Maggie Clancy, Isabel S. Wade, John J. Young
Plastic pollution negatively affects ecosystems and human health globally, with single-use plastic representing the majority of marine litter in some areas. Life science laboratories prefer pristine conditions for experimental reliability and therefore make use of factory standardized single-use plastic products. This contributes to overall plastic waste in the United States and globally. Here, we
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Macrophage polarization in THP-1 cell line and primary monocytes: A systematic review Differentiation (IF 2.9) Pub Date : 2022-10-26 Zahidah Nasuha Mohd Yasin, Fatin Najiah Mohd Idrus, Chee Hock Hoe, Get Bee Yvonne-Tee
Macrophages derived from human monocytic leukemia THP-1 cell line are often used as the alternative of human primary macrophage. However, the polarization method of THP-1 to macrophages varies between different laboratories, which may unknowingly affect the relevance of research output across research groups. In this regard, a systematic search was developed in Pubmed, BioOne, Scopus, and Science Direct
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Development of the human ovary: Fetal through pubertal ovarian morphology, folliculogenesis and expression of cellular differentiation markers Differentiation (IF 2.9) Pub Date : 2022-10-19 Maya R. Overland, Yi Li, Amber Derpinghaus, Sena Aksel, Mei Cao, Nicholas Ladwig, Gerald R. Cunha, Marta Himelreich-Perić, Laurence S. Baskin
A definition of normal human fetal and early postnatal ovarian development is critical to the ability to accurately diagnose the presence or absence of functional ovarian tissue in clinical specimens. Through assembling an extensive histologic and immunohistochemical developmental ontogeny of human ovarian specimens from 8 weeks of gestation through 16 years of postnatal, we present a comprehensive
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Early neurogenic properties of iPSC-derived neurosphere formation in Japanese macaque monkeys Differentiation (IF 2.9) Pub Date : 2022-10-14 Risako Nakai, Yusuke Hamazaki, Haruka Ito, Masanori Imamura
Non-human primates are important models for investigations of neural development and evolution, and the use of Japanese macaque monkeys has especially contributed to the advancement of neuroscience studies. However, these studies are restricted by the number of animals able to be evaluated and the invasiveness of the methodologies. Induced pluripotent stem cells (iPSCs) can provide an alternative strategy
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Interferon-beta induces major histocompatibility complex of class I (MHC-I) expression and a proinflammatory phenotype in cultivated human astrocytes Differentiation (IF 2.9) Pub Date : 2022-10-15 Raffaela Silvestre Ignarro, André Luis Bombeiro, Gabriela Bortolança Chiarotto, Luciana Politti Cartarozzi, Lilian de Oliveira Coser, Enrico Ghizoni, Helder Tedeschi, Fernando Cendes, Iscia Lopes-Cendes, Fabio Rogerio, Alexandre Leite Rodrigues de Oliveira
Major histocompatibility complex class I (MHC-I) has been implicated in several types of neuroplasticity phenomena. Interferon beta-1b (IFN-β) increases MHC-I expression by motoneurons after sciatic nerve crush in mice, improving axonal growth and functional recovery. Additionally, IFN-β induces glial hypertrophy associated with upregulation of glial fibrillary acidic protein (GFAP) and MHC-I in murine