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Retraction notice to “Gelatin scaffold combined with bone morphogenetic protein-4 induces odontoblast-like cell differentiation involving integrin profile changes, autophagy-related gene 10, and Wnt5 sequentially in human induced pluripotent stem cells” [Differentiation 93 (2017) 1–14] Differentiation (IF 2.392) Pub Date : 2021-01-13 Nobuaki Ozeki; Naoko Hase; Naoya Higuchi; Taiki Hiyama; Hideyuki Yamaguchi; Rie Kawai; Toru Matsumoto; Kazuhiko Nakata; Makio Mogi
This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/our-business/policies/article-withdrawal). This article has been retracted at the request of the authors, with the approval of the Editor-in-Chief. The journal was initially contacted by the corresponding author to report the unavailability of the raw data of the results presented by the
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Developmental estrogenization: Prostate gland reprogramming leads to increased disease risk with aging Differentiation (IF 2.392) Pub Date : 2021-01-07 Gail S. Prins
While estrogens are involved in normal prostate morphogenesis and function, inappropriate early-life estrogenic exposures, either in type, dose or timing, can reprogram the prostate gland and lead to increased disease risk with aging. This process is referred to as estrogen imprinting or developmental estrogenization of the prostate gland. The present review discusses published and new evidence for
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Estrogens and development of the rete testis, efferent ductules, epididymis and vas deferens Differentiation (IF 2.392) Pub Date : 2020-12-13 Rex A. Hess; Richard M. Sharpe; Barry T. Hinton
Estrogen has always been considered the female hormone and testosterone the male hormone. However, estrogen's presence in the testis and deleterious effects of estrogen treatment during development have been known for nearly 90 years, long before estrogen receptors (ESRs) were discovered. Eventually it was learned that testes actually synthesize high levels of estradiol (E2) and sequester high concentrations
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Role of nuclear and membrane estrogen signaling pathways in the male and female reproductive tract Differentiation (IF 2.392) Pub Date : 2020-12-09 Paul S. Cooke; Ana M. Mesa; Vijay K. Sirohi; Ellis R. Levin
Estrogen signaling through the main estrogen receptor, estrogen receptor 1 (ESR1; also known as ERα), is essential for normal female and male reproductive function. Historically, studies of estrogen action have focused on the classical genomic pathway. Although this is clearly the major pathway for steroid hormone actions, these hormones also signal through rapid non-classical effects involving cell
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Gestational folate deficiency alters embryonic gene expression and cell function Differentiation (IF 2.392) Pub Date : 2020-11-27 R.S. Seelan; P. Mukhopadhyay; J. Philipose; R.M. Greene; M.M. Pisano
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Anatomy of the mouse penis and internal prepuce Differentiation (IF 2.392) Pub Date : 2020-09-30 Gerald R. Cunha; Mei Cao; Adriane Sinclair; Amber Derpinghaus; Laurence S. Baskin
This paper addresses a confusing issue of preputial anatomy of the mouse. The term “internal prepuce” was used in 2013 to describe a preputial structure integral to the mouse glans penis. Subsequently in 2015 the same term was applied by another group to describe entirely different morphology, generating confusion in the literature. Because it is inappropriate to use the same term to describe entirely
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CircRNAs and LncRNAs in Osteoporosis Differentiation (IF 2.392) Pub Date : 2020-10-28 Tiantian He; Wei Liu; Lihua Cao; Ying Liu; Zi Zou; Yancheng Zhong; Haihua Wang; Yuqing Mo; Shuping Peng; Cijun Shuai
Osteoporosis is a systemic bone disease with bone fragility and increased fracture risk. The non-coding RNAs (ncRNAs) have appeared as important regulators of cellular signaling and pertinent human diseases. Studies have demonstrated that circular RNAs (circRNAs) and long non-coding RNAs (lncRNAs) are involved in the progression of osteoporosis through a variety of pathways, and are considered as targets
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New frontiers of developmental endocrinology opened by researchers connecting irreversible effects of sex hormones on developing organs Differentiation (IF 2.392) Pub Date : 2020-10-31 Taisen Iguchi; Tomomi Sato; Tadaaki Nakajima; Shinichi Miyagawa; Noboru Takasugi
In the early 1960's, at Professor Bern's laboratory, University of California, Berkeley, University of California, Berkeley) in the US, Takasugi discovered ovary-independent, persistent vaginal changes in mice exposed neonatally to estrogen, which resulted in vaginal cancer later in life. Reproductive abnormalities in rodents were reported as a result of perinatal exposure to various estrogenic chemicals
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The involvement of hsa_circ_0000417 in the development of hypospadias by regulating AR Differentiation (IF 2.392) Pub Date : 2020-10-14 Chao chen; Yong-yuan Jian; Xiang-you Zhao; Yu-ling Liu; Qi-ke Xie
Background Congenital hypospadias is a common congenital malformation of the urinary system in male children. However, the role of circRNA in congenital hypospadias remains unknown. Methods Differentially expressed circRNAs and mRNAs were identified by RNA sequencing. GO and KEEG analysis were performed to uncover the key function and pathways. The interaction networks were constructed and analyzed
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Ontogeny of estrogen receptors in human male and female fetal reproductive tracts Differentiation (IF 2.392) Pub Date : 2020-10-17 Gerald R. Cunha; Yi Li Cao, Mei; Amber Derpinghaus; Laurence S. Baskin
This paper reviews and provides new observations on the ontogeny of estrogen receptor alpha (ESR1) and estrogen receptor beta (ESR2) in developing human male and female internal and external genitalia. Included in this study are observations on the human fetal uterine tube, the uterotubal junction, uterus, cervix, vagina, penis and clitoris. We also summarize and report on the ontogeny of estrogen
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LINC00968 promotes osteogenic differentiation in vitro and bone formation in vivo via regulation of miR-3658/RUNX2 Differentiation (IF 2.392) Pub Date : 2020-10-09 Chunhui Liao; Yutao Zhou; Mingfei Li; Yixin Xia; Wei Peng
Osteogenic differentiation of dental pulp stem cells (DPSCs) is considered as a promising strategy in posterior maxilla tooth implantation. Information on the function and mechanisms of long non-coding RNAs (lncRNAs) in osteogenic differentiation of DPSCs is growing, however, the mechanism of LINC00968 and miR-3658 in regulating osteogenic differentiation of DPSCs still needs to be explored. In this
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Estrogens and development of the mouse and human external genitalia Differentiation (IF 2.392) Pub Date : 2020-10-01 Laurence Baskin; Adriane Sinclair; Amber Derpinghaus; Mei Cao; Yi Li; Maya Overland; Sena Askel; Gerald R. Cunha
The Jost hypothesis states that androgens are necessary for normal development of the male external genitalia. In this review, we explore the complementary hypothesis that estrogens can elicit abnormal development of male external genitalia. Herein, we review available data in both humans and mice on the deleterious effects of estrogen on external genitalia development, especially during the “window
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Murine in vitro cellular models to better understand adipogenesis and its potential applications. Differentiation (IF 2.392) Pub Date : 2020-08-23 Muhammad Sufyan Vohra,Bilal Ahmad,Christopher J Serpell,Ishwar S Parhar,Eng Hwa Wong
Adipogenesis has been extensively studied using in vitro models of cellular differentiation, enabling long-term regulation of fat cell metabolism in human adipose tissue (AT) material. Many studies promote the idea that manipulation of this process could potentially reduce the prevalence of obesity and its related diseases. It has now become essential to understand the molecular basis of fat cell development
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A uniform expression library for the exploration of FOX transcription factor biology. Differentiation (IF 2.392) Pub Date : 2020-08-20 Lavanya Moparthi,Stefan Koch
Forkhead box (FOX) family transcription factors play essential roles in development, tissue homeostasis, and disease. Although the biology of several FOX proteins has been studied in depth, it is unclear to what extent these findings apply to even closely related family members, which frequently exert overlapping but non-redundant functions. To help address this question, we have generated a uniform
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Sox17-mediated expression of adherent molecules is required for the maintenance of undifferentiated hematopoietic cluster formation in midgestation mouse embryos. Differentiation (IF 2.392) Pub Date : 2020-08-16 Satomi Takahashi,Ikuo Nobuhisa,Kiyoka Saito,Melig Gerel,Ayumi Itabashi,Kaho Harada,Mitsujiro Osawa,Takaho A Endo,Atsushi Iwama,Tetsuya Taga
Hematopoietic stem cell-containing intra-aortic hematopoietic cell clusters (IAHCs) emerge in the dorsal aorta of the aorta-gonad-mesonephros (AGM) region during midgestation mouse embryos. We previously showed that transduction of Sox17 in CD45lowc-Kithigh cells, which are one component of IAHCs, maintained the cluster formation and the undifferentiated state, but the mechanism of the cluster formation
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Fetal bovine serum-derived exosomes regulate the adipogenic differentiation of human bone marrow mesenchymal stromal cells in a cross-species manner. Differentiation (IF 2.392) Pub Date : 2020-07-25 Qiongfei Zhou,Fen Xie,Bin Zhou,Chan Li,Yijun Kang,Bo Wu,Lin Li,Ruchun Dai
Fetal bovine serum (FBS) contains a large number of exosomes which may disturb the analysis of exosomes derived from cultured cells. We investigated the effect of FBS-derived exosomes (FBS-Exos) on the adipogenic differentiation of human bone marrow mesenchymal stromal cells (hBM-MSCs) and the underlying molecular mechanism. The uptake of FBS-Exos by hBM-MSCs could be detected by the laser confocal
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Oxygen tension influences embryonic stem cell maintenance and has lineage specific effects on neural and cardiac differentiation. Differentiation (IF 2.392) Pub Date : 2020-07-22 Regina H Mennen,Victoria C de Leeuw,Aldert H Piersma
The importance of oxygen tension in in vitro cultures and its effect on embryonic stem cell (ESC) differentiation has been widely acknowledged. Research has mainly focussed on ESC maintenance or on one line of differentiation and only few studies have examined the potential relation between oxygen tension during ESC maintenance and differentiation. In this study we investigated the influence of atmospheric
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The cooperative effects of micro-grooved topography and TGF-β1 on the vascular smooth muscle cell contractile protein expression of the mesenchymal stem cells. Differentiation (IF 2.392) Pub Date : 2020-07-17 Sakhavat Abolhasani,Masoumeh Rajabibazl,Mohammad-Mehdi Khani,Azim Parandakh,Reyhaneh Hoseinpoor
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A comparison of prostatic development in xenografts of human fetal prostate and human female fetal proximal urethra grown in dihydrotestosterone-treated hosts. Differentiation (IF 2.392) Pub Date : 2020-07-14 Gerald R Cunha,Mei Cao,Omar Franco,Laurence S Baskin
The goal of this paper is to explore the ability of the human female urogenital sinus immediately below the bladder (proximal urethra) to undergo prostatic development in response to dihydrotestosterone (DHT). To establish this idea, xenografts of human fetal female proximal urethra were grown in castrated nude mouse hosts receiving a subcutaneous DHT pellet. To verify the prostatic nature of the resultant
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p53 positively regulates osteoprotegerin-mediated inhibition of osteoclastogenesis by downregulating TSC2-induced autophagy in vitro. Differentiation (IF 2.392) Pub Date : 2020-06-21 Xishuai Tong,Jianhong Gu,Miaomiao Chen,Tao Wang,Hui Zou,Ruilong Song,Hongyan Zhao,Jianchun Bian,Zongping Liu
Osteoclasts are terminally multinucleated cells that are regulated by nuclear factor-activated T cells c1 (NFATc1), and are responsible for bone resorption while the tartrate resistant acid phosphatase (TRAP) enzymes releases into bone resorption lacunae. Furthermore, tumor suppressor p53 is a negative regulator during osteoclastogenesis. Osteoprotegerin (OPG) inhibits osteoclastogenesis and bone resorption
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Changes in Nup62 content affect contact-induced differentiation of cultured myoblasts Differentiation (IF 2.392) Pub Date : 2020-05-19 Patrick J. Bishop, Yayoi Kinoshita, N. Natalie Lopes, Avery S. Ward, D. Stave Kohtz
Differentiation of cultured skeletal myoblasts is induced by extrinsic signals that include reduction in ambient mitogen concentration and increased cell density. Using an established murine myoblast cell line (C2C12), we have found that experimental reduction of the nucleoporin p62 (Nup62) content of myoblasts enhances differentiation in high-mitogen medium, while forced expression of Nup62 inhibits
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Analysis of hiPSCs differentiation toward hepatocyte-like cells upon extended exposition to oncostatin Differentiation (IF 2.392) Pub Date : 2020-05-19 Mathieu Danoy, Yannick Tauran, Stéphane Poulain, Hiroshi Arakawa, Daiki Mori, Karin Araya, Sachi Kato, Taketomo Kido, Hiroyuki Kusuhara, Yukio Kato, Atsushi Miyajima, Charles Plessy, Yasuyuki Sakai, Eric Leclerc
The capability to produce and maintain functional human adult hepatocytes remains one of the major challenges for the use of in-vitro models toward liver cell therapy and industrial drug-screening applications. Among the suggested strategies to solve this issue, the use of human-induced pluripotent stem cells (hiPSCs), differentiated toward hepatocyte-like cells (HLCs) is promising. In this work, we
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LncRNA-Gm2044 is transcriptionally activated by A-MYB and regulates Sycp1 expression as a miR-335-3p sponge in mouse spermatocyte-derived GC-2spd(ts) cells. Differentiation (IF 2.392) Pub Date : 2020-05-19 Meng Liang,Haiyan Wang,Chaofan He,Kejia Zhang,Ke Hu
Long noncoding RNAs (lncRNAs) have been shown to execute key roles in spermatogenesis. However, little is known about how lncRNAs gene expression is itself regulated in the germ cells of testis. We previously demonstrated that high expression of lncRNA-Gm2044 exists in spermatocytes and can regulate male germ cell proliferation. Here, the transcriptional regulation of lnRNA-Gm2044 expression in spermatocytes
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Comparison of the biological characteristics of umbilical cord mesenchymal stem cells derived from the human heterosexual twins Differentiation (IF 2.392) Pub Date : 2020-05-18 Yuanyuan Zhang, Pengju Lv, Yalong Li, Yonghui Zhang, Chaofei Cheng, Hongbo Hao, Han Yue
Mesenchymal stromal/stem cells (MSCs) are attracting more and more attention due to their tissue regenerative properties and immunomodulatory functions. MSCs may be the most acceptable, safe, and effective source for allogeneic cell therapy, and have been used in medical treatment. However, the similarities and differences between umbilical cord-derived MSCs (UC-MSCs) of heterosexual twins remain poorly
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p53 inhibits the osteogenic differentiation but does not induce senescence in human dental follicle cells Differentiation (IF 2.392) Pub Date : 2020-05-18 Oliver Pieles, Anja Reck, Torsten E. Reichert, Christian Morsczeck
Replicative senescence causes a reduced osteogenic differentiation potential of senescent dental follicle cells (DFCs). The transcription factor p53 is often involved in the induction of cellular senescence, but little is known about its role in DFCs. This study examined for the first time the role of p53 compared to its pro-proliferative antagonist E2F-1 in terms of osteogenic differentiation potential
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Laminins in osteogenic differentiation and pluripotency maintenance Differentiation (IF 2.392) Pub Date : 2020-05-16 Chaozhen Chen, Zhiwei Jiang, Guoli Yang
Extracellular matrix (ECM) plays an integral role in different developmental stages and in multiple systems. However, due to ECM being composed of various extracellular components (growth factors, cytokines, and hormones), its innate complexity and the lack of any efficient purification techniques limit further research into the detailed mechanisms of its role in cellular activities. Laminin (LN),
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Maternal control of early patterning in sea urchin embryos Differentiation (IF 2.392) Pub Date : 2020-04-22 Yulia O. Kipryushina, Konstantin V. Yakovlev
Sea urchin development has been studied extensively for more than a century and considered regulative since the first experimental evidence. Further investigations have repeatedly supported this standpoint by revealing the presence of inductive mechanisms that alter cell fate decisions at early cleavage stages and flexibility of development in response to environmental conditions. Some features indicate
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The multiple roles of Thy-1 in cell differentiation and regeneration Differentiation (IF 2.392) Pub Date : 2020-04-08 Jie Yang, Xiao-Zhen Zhan, Jonathan Malola, Zhen-Yan Li, Jogendra Singh Pawar, Huan-Tian Zhang, Zhen-Gang Zha
Thy-1 is a 25–37 kDa glycophosphatidylinositol (GPI)-anchored cell surface protein that was discovered more than 50 years ago. Recent findings have suggested that Thy-1 is expressed on thymocytes, mesenchymal stem cells (MSCs), cancer stem cells, hematopoietic stem cells, fibroblasts, myofibroblasts, endothelial cells, neuronal smooth muscle cells, and pan T cells. Thy-1 plays vital roles in cell migration
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Spatial and temporal deletion reveals a latent effect of Megf8 on the left-right patterning and heart development Differentiation (IF 2.392) Pub Date : 2020-03-14 Wenfeng Wang, Xiaoling Zheng, Hejie Song, Junjie Yang, Xiangyang Liu, Ye Wang, Min Zhang, Zhen Zhang
Laterality disease is frequently associated with congenital heart disease (CHD). However, it is unclear what is behind this association, a pleiotropic effect of common genetic causes of laterality diseases or the impact of abnormal left-right patterning on the downstream cardiovascular development. MEGF8 is a disease gene of Carpenter syndrome characterized by defective lateralization and CHD. Here
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Roles of circular RNAs in regulating the self-renewal and differentiation of adult stem cells Differentiation (IF 2.392) Pub Date : 2020-03-06 Yang Wang, Zhiwei Jiang, Mengjia Yu, Guoli Yang
Circular RNAs (circRNAs) are a type of noncoding RNA (ncRNA) and have attracted widespread research attention in recent years. They form a covalently closed loop structure and are widely distributed in tissues and organs. CircRNAs have also been found to regulate a variety of biological processes by functioning as microRNA sponges, transcriptional regulators, and protein binding partners. Recent sequencing
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The RB gene family controls the maturation state of the EndoC-βH2 human pancreatic β-cells Differentiation (IF 2.392) Pub Date : 2020-02-24 Alicia Maugein, Marc Diedisheim, Karine Bailly, Raphaël Scharfmann, Olivier Albagli
The functional maturation of human pancreatic β-cells remains poorly understood. EndoC-βH2 is a human β-cell line with a reversible immortalized phenotype. Removal of the two oncogenes, SV40LT and hTERT introduced for its propagation, stops proliferation, triggers cell size increase and senescence, promotes mitochondrial activity and amplifies several β-cell traits and functions. Overall, these events
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Reprogramming of chimpanzee fibroblasts into a multipotent cancerous but not fully pluripotent state by transducing iPSC factors in 2i/LIF culture Differentiation (IF 2.392) Pub Date : 2020-02-04 Zachary Yu-Ching Lin, Risako Nakai, Hirohisa Hirai, Daiki Kozuka, Seiya Katayama, Shin-ichiro Nakamura, Sawako Okada, Ryunosuke Kitajima, Hiroo Imai, Hideyuki Okano, Masanori Imamura
To induce and maintain naïve pluripotency in mouse embryonic and induced pluripotent stem cells (ESCs/iPSCs), chemically defined N2B27 medium with PD0325901, CHIR99021, and leukemia inhibitory factor (2i/LIF) is a classic and simple condition. However, this method cannot be simply extrapolated to human ESCs/iPSCs that are principally stabilized in primed pluripotency and become primitive neuroepithelium-like
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Non-viral reprogramming and induced pluripotent stem cells for cardiovascular therapy Differentiation (IF 2.392) Pub Date : 2020-01-10 Arunima Panda, Narasimman Gurusamy, Sheeja Rajasingh, Hannah-Kaye Carter, Edwin L. Thomas, Johnson Rajasingh
Despite significant effort devoted to developing new treatments and procedures, cardiac disease is still one of the leading causes of death in the world. The loss of myocytes due to ischemic injury remains a major therapeutic challenge. However, cell-based therapy to repair the injured heart has shown significant promise in basic and translation research and in clinical trials. Embryonic stem cells
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Periodontal ligament cells regulate osteogenesis via miR-299-5p in mesenchymal stem cells Differentiation (IF 2.392) Pub Date : 2020-01-09 Eri Kaneda-Ikeda, Tomoyuki Iwata, Noriyoshi Mizuno, Takayoshi Nagahara, Mikihito Kajiya, Katsuhiro Takeda, Reika Hirata, Shu Ishida, Minami Yoshioka, Tsuyoshi Fujita, Hiroyuki Kawaguchi, Hidemi Kurihara
Background The periodontal ligament contains periodontal ligament cells, which is a heterogeneous cell population, and includes progenitor cells that can differentiate into osteoblasts/cementoblasts. Mesenchymal stem cells (MSCs) can differentiate into various cells and can be used for periodontal regenerative therapy. Therefore, transplanted MSCs can be affected by humoral factors from periodontal
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Expression analysis of microRNAs and mRNAs in myofibroblast differentiation of lung resident mesenchymal stem cells Differentiation (IF 2.392) Pub Date : 2019-12-10 Cong Wang, Honghui Cao, Shen Gu, Chaowen Shi, Xiang Chen, Xiaodong Han
Idiopathic pulmonary fibrosis (IPF) is a serious lung disease that involved the myofibroblast differentiation of lung resident mesenchymal stem cells (LR-MSCs). However, the specific molecular mechanisms of myofibroblast differentiation of LR-MSCs still remain a mystery. In this study, a comprehensive analysis of miRNAs and mRNAs changes in LR-MSCs treated with TGF-β1 was performed. Through computational
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Metabolomic profiling during the differentiation of human induced pluripotent stem cells into hepatocyte-like cells Differentiation (IF 2.392) Pub Date : 2019-12-10 Rachid Jellali, Myriam Lereau Bernier, Yannick Tauran, Françoise Gilard, Mathieu Danoy, Taketomo Kido, Atsushi Miyajima, Yasuyuki Sakai, Eric Leclerc
Human induced pluripotent stem cells (hiPSCs) are potentially an invaluable source of cells for regenerative medicine, disease modeling and drug discovery. However, the differentiation of hiPSCs into fully functional hepatocytes remains a major challenge. Despite the importance of the information carried by metabolomes, the exploitation of metabolomics for characterizing and understanding hiPSC differentiation
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A novel long non-coding RNA, Leat1, causes reduced anogenital distance and fertility in female mice Differentiation (IF 2.392) Pub Date : 2019-12-04 Deidre Mattiske, Richard R. Behringer, Paul A. Overbeek, Andrew J. Pask
Defective anorectal and urogenital malformations are some of the most severe congenital anomalies encountered in children. Only a few molecular cues have been identified in early formation of the female urogenital system. Here we describe a novel long non-coding RNA molecule known as Leat1 (long non-coding RNA, EphrinB2 associated transcript 1). This lncRNA is syntenic with EfnB2 (which encodes EphrinB2)
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Hot spots in fetal human penile and clitoral development Differentiation (IF 2.392) Pub Date : 2019-12-04 Laurence Baskin, Amber Derpinghaus, Mei Cao, Adriane Sinclair, Yi Li, Maya Overland, Gerald R. Cunha
To better understand how the human fetal penis and clitoris grows and remodels, we undertook an investigation to define active areas of cellular proliferation and programmed cell death spatially and temporally during development of human fetal external genitalia from the indifferent stage (8 weeks) to 18 weeks of gestation. Fifty normal human fetal penile and clitoral specimens were examined using
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A crucial role of fibroblast growth factor 2 in the differentiation of hair follicle stem cells toward endothelial cells in a STAT5-dependent manner Differentiation (IF 2.392) Pub Date : 2019-10-25 Bingjie Cai, Yunpeng Zheng, Xiaojun Liu, Jiadi Yan, Junmin Wang, Guangwen Yin
Fibroblast growth factor (FGF2) is reported to affect the proliferation, differentiation, and survival abilities of stem cells. In this study, we hypothesize that FGF2 might promote the differentiation of hair follicle stem cell (HFSCs) into endothelial cells (ECs), in a manner dependent on STAT5 activation. We first treated human HFSCs with recombinant human FGF2 to determine the involvement of FGF2
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SHED-derived conditioned exosomes enhance the osteogenic differentiation of PDLSCs via Wnt and BMP signaling in vitro Differentiation (IF 2.392) Pub Date : 2019-10-13 Menghong Wang, Jie Li, Yanyan Ye, Songlin He, Jinlin Song
The exosomes from human exfoliated deciduous teeth (SHED-Exos) have exhibited potential therapeutic role in dental and oral disorders. The biological effects of exosomes largely depend on cellular origin and physiological status of donor cell. In the present study, we explored the influence of conditioned exosomes from SHED with osteogenic induction on periodontal ligament stem cells (PDLSCs) in vitro
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Imaging the developing human external and internal urogenital organs with light sheet fluorescence microscopy Differentiation (IF 2.392) Pub Date : 2019-10-10 Dylan Isaacson, Dylan McCreedy, Meredith Calvert, Joel Shen, Adriane Sinclair, Mei Cao, Yi Li, Todd McDevitt, Gerald Cunha, Laurence Baskin
Technological advances in three-dimensional (3D) reconstruction techniques have previously enabled paradigm shifts in our understanding of human embryonic and fetal development. Light sheet fluorescence microscopy (LSFM) is a recently-developed technique that uses thin planes of light to optically section whole-mount cleared and immunolabeled biologic specimens. The advent of commercially-available
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Development of the human prepuce and its innervation Differentiation (IF 2.392) Pub Date : 2019-10-10 Gerald R. Cunha, Adriane Sinclair, Mei Cao, Laurence S. Baskin
Development of the human prepuce was studied over the course of 9–17 weeks of gestation in 30 specimens. Scanning electron microscopy revealed subtle surface features that were associated with preputial development, namely the appearance of epidermal aggregates that appeared to be associated with formation of the preputial fold. Transverse and sagittal sections revealed that the epidermis of the glans
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SMAD2 overexpression rescues the TGF-β3 null mutant mice cleft palate by increased apoptosis Differentiation (IF 2.392) Pub Date : 2019-10-08 Abdullah M. AlMegbel, Charles F. Shuler
During palatal development, medial edge epithelium (MEE) disappearance is one of the crucial steps in the process of fusion. The fate of these cells is still debated, and controversies remain. During secondary palate fusion, TGF-β3 signaling mediated in the cell through the SMAD2 protein plays an important role and leads to the disappearance of the midline epithelial seam (MES) and the confluence of
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Enhanced chondrogenic differentiation of human mesenchymal stem cells in silk fibroin/chitosan/glycosaminoglycan scaffolds under dynamic culture condition Differentiation (IF 2.392) Pub Date : 2019-09-19 Parinita Agrawal, Krishna Pramanik
Cartilage tissue damage and diseases are the most common clinical situation that occurs because of aging and injury, thereby causing pain and loss of mobility. The inability of cartilage tissue to self-repair is instrumental in developing tissue engineered substitutes. To this effect, the present study aims to engineer cartilage construct by culturing umbilical cord blood-derived human mesenchymal
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Regulatory roles of epithelial-mesenchymal interaction (EMI) during early and androgen dependent external genitalia development Differentiation (IF 2.392) Pub Date : 2019-09-11 Taiju Hyuga, Kentaro Suzuki, Alvin R. Acebedo, Daiki Hashimoto, Mizuki Kajimoto, Shinichi Miyagawa, Jun-ichiro Enmi, Yoshichika Yoshioka, Gen Yamada
Development of external genitalia (ExG) has been a topic of long mystery in the field of organogenesis research. Early stage male and female of mouse embryos develop a common genital tubercle (GT) in the perineum whose outgrowth extends distally from the posterior cloacal regions. Concomitant with GT outgrowth, the cloaca is divided into urogenital sinus and anorectum by urorectal septum (URS) internally
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Temporal, spatial, and genetic regulation of external genitalia development Differentiation (IF 2.392) Pub Date : 2019-09-10 Meade Haller, Liang Ma
Fertilization requires the physical combination of gametes, and terrestrial mammals necessitated the evolution of genitalia capable of successfully completing the fertilization process in a non-aqueous environment. Thus, the male mammalian external genitalia evolved as an outgrowth from the body, an appendage sufficient to fertilize eggs housed deep inside the female. In this way, sexual dimorphism
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Clitoral development in the mouse and human Differentiation (IF 2.392) Pub Date : 2019-09-07 Gerald R. Cunha, Ge Liu, Adriane Sinclair, Mei Cao, Laurence Baskin
The goal of this report is (a) to provide the first detailed description of mouse clitoral development, and (b) to compare mouse and human clitoral development. For this purpose, external genitalia of female mice were examined by wholemount microscopy, histology and immunohistochemistry from 14 days of gestation to 10 days postnatal. Human clitoral development was examined by these techniques as well
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Androgen and estrogen receptor expression in the developing human penis and clitoris Differentiation (IF 2.392) Pub Date : 2019-09-06 Laurence Baskin, Mei Cao, Adriane Sinclair, Yi Li, Maya Overland, Dylan Isaacson, Gerald R. Cunha
Recent evidence suggest that estrogens along with androgens are necessary for male sexual differentiation, augmenting Jost’s original hypothesis. To better understand the mechanism of action of androgens and estrogens in human genital development, we undertook an investigation to define the precise location of the androgen receptor (AR), estrogen receptors alpha (ERα) and beta (ERβ) in the developing
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Androgen-independent events in penile development in humans and animals Differentiation (IF 2.392) Pub Date : 2019-09-06 Gerald R. Cunha, Ge Liu, Adriane Sinclair, Mei Cao, Steve Glickman, Paul S. Cooke, Laurence Baskin
The common view on penile development is that it is androgen-dependent, based first and foremost on the fact that the genital tubercle forms a penis in males and a clitoris in females. However, critical examination of the complex processes involved in human penile development reveals that many individual steps in development of the genital tubercle are common to both males and females, and thus can
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Reproductive tract biology: Of mice and men Differentiation (IF 2.392) Pub Date : 2019-09-06 Gerald R. Cunha, Adriane Sinclair, Will A. Ricke, Stanley J. Robboy, Mei Cao, Laurence S. Baskin
The study of male and female reproductive tract development requires expertise in two separate disciplines, developmental biology and endocrinology. For ease of experimentation and economy, the mouse has been used extensively as a model for human development and pathogenesis, and for the most part similarities in developmental processes and hormone action provide ample justification for the relevance
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Extensive apoptosis during the formation of the terminal nerve ganglion by olfactory placode-derived cells with distinct molecular markers Differentiation (IF 2.392) Pub Date : 2019-09-06 Tamilarasan K. Palaniappan, Lina Slekiene, Lena Gunhaga, Cedric Patthey
The terminal nerve ganglion (TNG) is a well-known structure of the peripheral nervous system in cartilaginous and teleost fishes. It derives from the olfactory placode during embryonic development. While the differentiation and migration of gonadotropin releasing hormone (GnRH)-expressing neurons from the olfactory placode has been well documented, the TNG has been neglected in birds and mammals, and
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Comparing the use of differentiated adipose-derived stem cells and mature adipocytes to model adipose tissue in vitro Differentiation (IF 2.392) Pub Date : 2019-09-06 Ann-Cathrin Volz, Birgit Omengo, Sandra Gehrke, Petra Juliane Kluger
In vitro models of human adipose tissue may serve as beneficial alternatives to animal models to study basic biological processes, identify new drug targets, and as soft tissue implants. With this approach, we aimed to evaluate adipose-derived stem cells (ASC) and mature adipocytes (MA) comparatively for the application in the in vitro setup of adipose tissue constructs to imitate native adipose tissue
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A loss of estrogen signaling in the aromatase deficient mouse penis results in mild hypospadias Differentiation (IF 2.392) Pub Date : 2019-09-06 Samuel M. Cripps, Deidre M. Mattiske, Jay R. Black, Gail P. Risbridger, Luke C. Govers, Tiffany R. Phillips, Andrew J. Pask
Hypospadias is the abnormal opening of the urethra on the underside of the penis and occurs in approximately 1/125 live male births worldwide. The incidence rate of hypospadias has dramatically increased over the past few decades. This is now attributed, at least in part, to our exposure to endocrine-disrupting chemicals (EDCs) which alter the hormonal signals required for development of the penis
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Gremlin-1 potentiates the dedifferentiation of VSMC in early stages of atherosclerosis Differentiation (IF 2.392) Pub Date : 2019-08-30 Renata Silvério de Barros, Grazielle Suhett Dias, Ana Paula do Rosario, Fernanda Vieira Paladino, Gabriel Herculano Lopes, Alexandre Holthausen Campos
Vascular smooth muscle cells (VSMC) are highly specialized, and exhibit a contractile phenotype when mature and fully differentiated, being responsible for vessel homeostasis and blood pressure control. In response to pro-atherogenic stimuli VSMC alter their state of differentiation, increase proliferation and migration, resulting in SMC phenotypes ranging from contractile to synthetic. This variability
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Close association between vasa-positive germ plasm granules and mitochondria correlates with cytoplasmic localization of 12S and 16S mtrRNAs during zebrafish spermatogenesis Differentiation (IF 2.392) Pub Date : 2019-08-30 Arkadiy Reunov, Konstantin Yakovlev, Jack Hu, Yulia Reunova, Alina Komkova, Yana Alexandrova, Evgenia Pimenova, Jens Tiefenbach, Henry Krause
The phenomenon of the cytoplasmic localisation of mitochondrial ribosomal subunits (12 S mitochondrial rRNA and 16 S mitochondrial rRNA) has been discovered by scientific teams working with spermatogenic cells of mice. Previous reports showed that the release of mitochondrial substance occurs during interaction of mitochondria with the germ plasm granules (GG). To determine if the interplay between
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A charged multivesicular body protein (CHMP4B) is required for lens growth and differentiation Differentiation (IF 2.392) Pub Date : 2019-07-31 Yuefang Zhou, Thomas M. Bennett, Alan Shiels
Charged multivesicular body protein 4B (CHMP4B) functions as a core component of the endosome sorting complex required for transport-III (ESCRT-III) machinery that facilitates diverse membrane remodeling and scission processes in eukaryotes. Mutations in the human CHMP4B gene underlie rare, inherited forms of early-onset lens opacities or cataract. Here we have characterized the lens phenotypes of
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Inhibiting effect of microRNA-187-3p on osteogenic differentiation of osteoblast precursor cells by suppressing cannabinoid receptor type 2 Differentiation (IF 2.392) Pub Date : 2019-07-13 Aihua Xu, Yang Yang, Yang Shao, Meng Wu, Yongxin Sun
Induction of osteoblast differentiation is an effective approach in promoting osteoblastogenesis and bone formation. MicroRNA (miR) is a kind of small regulatory RNA molecules that control both physiological and pathological processes. The purpose of this research was to explore the function of miR-187-3p in proliferation and osteogenic differentiation in human osteoblastic precursor cells (hFOB 1
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Improved differentiation of human enriched CD133+CD24+ renal progenitor cells derived from embryonic stem cell with embryonic mouse kidney-derived mesenchymal stem cells co-culture Differentiation (IF 2.392) Pub Date : 2019-07-10 Ehsan Ehsani, Soroosh Shekarchian, Hossein Baharvand, Nasser Aghdami, Reza Moghadasali
End-stage renal disease (ESRD) is a major global public health issue. In the past decade, regenerative medicine and cell-based therapies were recommended for treatment of devastating diseases like ESRD. Renal progenitor (RP) cells are essential players in such treatment approaches. The major practical difficulties in application of RP cells are generation of these cells and preservation of their self-renewal
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Molecular regulatory networks of thymic epithelial cell differentiation Differentiation (IF 2.392) Pub Date : 2019-06-14 Rong Luan, Zhanfeng Liang, Qian Zhang, Liguang Sun, Yong Zhao
Functional mature T cells are generated in the thymus. Thymic epithelial cells (TECs) provide the essential microenvironment for T cell development and maturation. According to their function and localization, TECs are roughly divided into cortical TECs (cTECs) and medullary TECs (mTECs), which are responsible for positive and negative selection, respectively. This review summarizes the current understanding
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Dickkopf1: An immunomodulatory ligand and Wnt antagonist in pathological inflammation Differentiation (IF 2.392) Pub Date : 2019-06-12 Wook-Jin Chae, Alfred L.M. Bothwell
The Wnt signaling pathway plays essential roles in tissue or organ homeostasis by regulating cell proliferation and differentiation. Upon tissue or organ injury, inflammation is coupled with tissue repair and regeneration process. The canonical Wnt signaling transduction pathway is crucial for cell proliferation, cell differentiation, and tissue regeneration. Dickkopf1 (DKK1) is a quintessential Wnt