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Locally Controlled Diffusive Release of Bone Morphogenetic Protein-2 Using Micropatterned Gelatin Methacrylate Hydrogel Carriers Biochip J. (IF 2.309) Pub Date : 2020-11-18 Myong-Hee Yi, Ji-Eun Lee, Chang-Beom Kim, Keun-Woo Lee, Kwang-Ho Lee
In this work, a novel and simple bone morphogenetic protein (BMP)-2 carrier is developed, which enables localized and controlled release of BMP-2 and facilitates bone regeneration. BMP-2 is localized in the gelatin methacrylate (GelMA) micropatterns on hydrophilic semi-permeable membrane (SNM), and its controlled release is regulated by the concentration of GelMA hydrogel and BMP-2. The controlled
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Verification of Operating Principle of Nano Field-effect Transistor Biosensor with an Extended Gate Electrode Biochip J. (IF 2.309) Pub Date : 2020-11-18 Hye-Lim Kang, Sumi Yoon, Dong-ki Hong, Sunga Song, Young Joo Kim, Won-Hyo Kim, Woo-Kyeong Seong, Kook-Nyung Lee
Many studies have been conducted on the use of nano field-effect transistor (nanoFET) sensors for the detection of biological species. However, the practical application of nanoFET-based biosensors is difficult because their operating principle has not been clarified. Most existing studies focused on ion concentration and pH level in a solution, the Debye length (the physical distance at which charged
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Recent Development of Aptasensor for Influenza Virus Detection Biochip J. (IF 2.309) Pub Date : 2020-11-16 Soo Min Kim, Jinmyeong Kim, Seungwoo Noh, Hiesang Sohn, Taek Lee
In nowadays, we have entered the new era of pandemics and the significance of virus detection deeply impacts human society. Viruses with genetic mutations are reported nearly every year, and people have prepared tools to detect the virus and vaccines to ensure proper treatments. Influenza virus (IV) is one of the most harmful viruses reporting various mutations, sub-types, and rapid infection speed
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Multiplexed Diagnosis of Four Serotypes of Dengue Virus by Real-time RT-PCR Biochip J. (IF 2.309) Pub Date : 2020-11-16 Jiyeon Kim, Eung-Soo Hwang
The number of people at risk for dengue viral infection is about 50% of the worlds population. Dengue viral infection is classified as dengue fever and severe dengue depending on the severity. Severe dengue may be associated with infection by the different serotype from the primary infected serotype. The circulating epidemic serotypes have changed continuously and periodically. It is very important
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Osteogenesis and Chondrogenesis of Primary Rabbit Periosteal Cells under Non-uniform 2-Axial Tensile Strain Biochip J. (IF 2.309) Pub Date : 2020-11-10 Chih-Hao Chiu, Yun-Wen Tong, Jen-Fang Yu, Kin Fong Lei, Alvin Chao-Yu Chen
Periosteal cells are the major cell sources of skeletal progenitors for fracture callus. In order to promote bone repair and cartilage formation, besides the application of exogenous growth factors, physical stimulation is an alternative approach to guide cell differentiation. Investigation of appropriate conditions is essential for forming bone and cartilage. In this work, a membrane-type micro-system
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Simulation Guided Microfluidic Design for Multitarget Separation Using Dielectrophoretic Principle Biochip J. (IF 2.309) Pub Date : 2020-10-27 B. I. Mohamed Zackria Ansar, Vineet Tirth, Caffiyar Mohammed Yousuff, Neeraj Kumar Shukla, Saiful Islam, Kashif Irshad, K. O. Mohammed Aarif
Microfluidic technologies have emerged as a potential tool for point of care — diagnostics and therapeutics applications. Isolation of multi-targets (Cancer cells along with platelets, red blood cells (RBCs), white blood cells (WBCs), and antigen-presenting cells (APCs)) simultaneously is of great interest in drug discovery and medical diagnosis. By utilizing dielectrophoresis (DEP) effect inside the
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Simultaneous Multiplexed Detection of Protein and Metal Ions by a Colorimetric Microfluidic Paper-based Analytical Device Biochip J. (IF 2.309) Pub Date : 2020-10-27 Xiaolu Xiong, Junlin Zhang, Zhou Wang, Chenchen Liu, Wende Xiao, Junfeng Han, Qingfan Shi
In order to improve the efficiency of disease diagnosis and environmental monitoring, it is desirable to detect the concentration of proteins and metal ions simultaneously, since the current popular diagnostic platform can only detect proteins or metal ions independently. In this work, we developed a colorimetric microfluidic paper-based analytical device (µPAD) for simultaneous determination of protein
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Tradeoff Control of Multi-exposure Lithography for SU-8 Photochemical Reaction Channel Formation Biochip J. (IF 2.309) Pub Date : 2020-10-27 Qi-Ming Chen, Jin-Yun Zhou, Yi-Ming Hu, Qi Zheng
A method involving multi-exposure with low-power is presented to fabricate an SU-8 mold based on a digital micromirror device (DMD) maskless lithography with an LED source at a wavelength of 405 nm. The SU-8 mold is used for the fabrication of a PDMS concentration gradient generator (CGG). During a one-time exposure, it is easy to over-expose at the top and under-expose at the bottom of a thick SU-8
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Pig Sera-derived Anti-SARS-CoV-2 Antibodies in Surface Plasmon Resonance Biosensors Biochip J. (IF 2.309) Pub Date : 2020-10-22 Ji-Hong Bong, Tae-Hun Kim, Jaeyong Jung, Soo Jeong Lee, Jeong Soo Sung, Chang Kyu Lee, Min-Jung Kang, Hyun Ok Kim, Jae-Chul Pyun
Anti-coronavirusdisease-2019 (COVID-19; anti-severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2)) antibodies against nucleoprotein (NP) were purified from pig sera. Following the separation of the antibody fraction using a protein-A column, the final yield of the purified antibodies against SARS-CoV-2 NPs was estimated to be 0.26 ± 0.05 % (absolute amount of 143.4 ± 25.2 ng, n=5) from 1 mL
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Efficient Bond of PDMS and Printed Circuit Board with An Application on Continuous-flow Polymerase Chain Reaction Biochip J. (IF 2.309) Pub Date : 2020-10-22 Yongjia Chang, Hui You
A simple and efficient bond technique for integrating polydimethylsiloxane (PDMS) and printed circuit board (PCB) is presented in this paper by using half-cured PDMS technique. We take advantage of the mature PCB technology for fabrication of microchannel mold and heating electrodes leading to a cost-effective and mass manufacturing method. PDMS is applied for fabricating microfluidic chip by replica
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A Multi-Inlet Microfluidic Nozzle Head with Shape Memory Alloy-Based Switching for Biomaterial Printing with Precise Flow Control Biochip J. (IF 2.309) Pub Date : 2020-09-23 Karthick Mani, Wei-Chen Lin, Chun-Fang Wang, Bivas Panigrahi, Yong-Jin Wu, Cheng-Lung Wu, Chia-Yuan Chen
3D bioprinting is one of the rapidly evolving fields of tissue engineering where microengineering meets cells biology within an unprecedented precision to construct tissue structures of various forms with complexity. However, enabling simultaneous printing of heterogeneous biomaterial along with scaffold components through the currently available printers is still considered as a major challenge due
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Microsized 3D Hydrogel Printing System using Microfluidic Maskless Lithography and Single Axis Stepper Motor Biochip J. (IF 2.309) Pub Date : 2020-09-11 Jinsik Yoon, Wook Park
The 3D printing apparatus in conventional inkjet and stereolithography systems is limited to continuous fabrication of a microsized three-dimensional hydrogel composed of multiple substances. We present a micro three-dimensional printing system by combining a polydimethylsiloxane microfluidic channel through which various fluids can flow into a micro two-dimensional particle generation system, and
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Sensitivity Improvement in Electrochemical Immunoassays Using Antibody Immobilized Magnetic Nanoparticles with a Clean ITO Working Electrode Biochip J. (IF 2.309) Pub Date : 2020-09-11 Sunga Song, Young Joo Kim, Hye-Lim Kang, Sumi Yoon, Dong-Ki Hong, Won-Hyo Kim, Ik-Soo Shin, Woo Kyeong Seong, Kook-Nyung Lee
We report a dramatic improvement in the detection sensitivity of electrochemical immunosensors through the use of a magnetic nanoparticle for immunoassay reactions and an ITO working electrode for measurement of the electrochemical enzymatic reaction signals. In general, electrochemical sensors developed for immunoassay require the immobilization of capture antibodies on the surface of the working
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Droplet-based Synthesis of Homogeneous Gold Nanoparticles for Enhancing HRP-based ELISA Signals Biochip J. (IF 2.309) Pub Date : 2020-09-01 Ji Wook Choi, Young Jae Kim, Jong Min Lee, Jin-Ha Choi, Jeong-Woo Choi, Bong Geun Chung
Gold nanoparticles (AuNPs) have widely used for various biological applications, such as drug screening, photo-thermal therapy, and biosensing. In particular, the synthesis of AuNPs with narrow size distribution plays an important role in increasing the efficiency of nanoparticle-mediated biosensors. However, the conventional synthesis methods (e.g., citrate reduction method) still suffer from controlling
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An On-chip Chemiluminescent Immunoassay for Bacterial Detection using in Situ-synthesized Cadmium Sulfide Nanowires with Passivation Layers Biochip J. (IF 2.309) Pub Date : 2020-08-27 Hong-Rae Kim, Ji-Hong Bong, Jaeyong Jung, Jeong Soo Sung, Min-Jung Kang, Jae-Gwan Park, Jae-Chul Pyun
The passivation layers of an in situ-synthesized cadmium sulfide (CdS) nanowire (NW) photosensor were prepared for two reasons: (1) to improve the physical stability of the NW on an interdigitated electrode and (2) to enhance the immobilization efficiency of proteins for the on-chip immunoassay. The passivation layer was estimated to have suitable optical properties, and the photoresponse of photosensor
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Experimental Measurement of Particle Velocity in a High Reynolds Micro-channel Flow Biochip J. (IF 2.309) Pub Date : 2020-08-27 Jongin Choi, Sunghwan Jung
The present work is to provide experimental measurements of particle velocity in a microchannel flow at the Reynolds number of 10 and their respective particle positions in the channel where the velocities were measured. The particle position measurements which includes their depths were realized without an intricate optical set-up by controlling the particle path with multiple flow rates. The experimental
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Development of a Human Respiratory Mucosa-on-a-chip using Decellularized Extracellular Matrix Biochip J. (IF 2.309) Pub Date : 2020-08-27 Mi Hyun Lim, Min Jae Lim, Won-Soo Yun, Songwan Jin, Donghyun Lee, Do Hyun Kim, Sung Won Kim
The airway mucosa is the first point of exposure to all inhalant materials. It is possible to produce microenvironments functionally similar to airway by using different types of extracellular matrices around mucosal cells, such as an extracellular matrix decellularized from trachea. In this study, we introduced a novel human respiratory mucosa-on-a-chip model that uses decellularized tracheal extracellular
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A Numerical Study of Droplet Splitting using Different Spacers in EWOD Device Biochip J. (IF 2.309) Pub Date : 2020-08-19 Zhaolong Wang, Xiongheng Bian, Liguo Chen
In this paper, we investigated the droplet splitting using different spacers in the air. The droplets were manipulated by the EWOD force and the level set method was used for our numerical implementation. The results show that the property of spacer has the effect on the droplet splitting. Specifically, we showed that the re-merging occurred when split daughter droplets pass the sub-channels for the
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Flexible Chemical Sensors Using Signal Generation from Cyclodextrin-Analyte Interactions on Polymer Composites Biochip J. (IF 2.309) Pub Date : 2020-08-19 Joonwon Bae, Dong-Sik Shin, Ji-Hwan Ha, Yunjung Hwang, Chang-Soo Lee, Hye Jun Kim, Ayeon Jang, Sung-Hoon Park
We present the design of a chemical sensor and study the generation of electrical signals produced by the formation of a cyclodextrin inclusion complex. Flexible poly(dimethylsiloxane)/graphene hybrid composites were used as sensor substrates and sensing media, beta-cyclodextrin (beta-CD) molecules were incorporated using simple bio-coupling reactions. The feasibility of sensing molecules through the
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SERS-based Nanoplasmonic Exosome Analysis: Enabling Liquid Biopsy for Cancer Diagnosis and Monitoring Progression Biochip J. (IF 2.309) Pub Date : 2020-08-18 Jong Uk Lee, Soohyun Kim, Sang Jun Sim
Exosomes, membrane-bound vesicles having a diameter of 30–150nm, are secreted by most cell types, including tumor cells. These vesicles mediate intercellular communication by transferring bioactive molecules (including a variety of proteins and nucleic acids) from donor to recipient cells. Notably, tumor cells secrete more exosome into microenvironment than nontumoral cells. Tumor-derived exosomes
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Liquid-crystal-droplet-based Monitoring System for Water-soluble Inorganic Acidic Gases from the Atmosphere Biochip J. (IF 2.309) Pub Date : 2020-08-18 Zongfu An, Chang-Hyun Jang
A liquid crystal (LC)-based pH sensor for real-time monitoring of variations in localized pH values near the LC droplets was developed, and its applicability for the detection of acidic gases was explored. It was found that 4-cyano-4′-pentylbiphenyl (5CB), when doped with hexanoic acid (HA), shows a bright-fan-shaped (planar orientation of LCs) to dark-cross (homeotropic orientation of LCs) optical
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Simple Paper-based Liver Cell Model for Drug Screening Biochip J. (IF 2.309) Pub Date : 2020-06-12 Kattika Kaarj; Jennifer Ngo; Christina Loera; Patarajarin Akarapipad; Soohee Cho; Jeong-Yeol Yoon
Investigation of the potential adverse effects of chemicals and drugs is essential during the drug development process. In vitro cell model systems have been developed over the past years towards such toxicity investigation. 96-well plate is the common platform for screening drug toxicity due to its simplicity. However, this platform only offers 2D cell culture environment and lacks the flow of solutions
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Development of Detection Methods for Zinc Pyrithione in Polypropylene via Simple Extraction Methods for Quality Control Biochip J. (IF 2.309) Pub Date : 2020-06-11 Chan Yeong Park; Sunhee Moon; Seung Hoon Baek; Min Woo Kim; Jihyeok Roh; Jeshin Sung; Tae Jung Park
Zinc pyrithione (ZPT) is the antimicrobial substance and has been applied for various fields, including non-fouling coating paints and cosmetics. It also used for the tub part of the electronic machine with polypropylene (PP). Since the released ZPT can cause skeletal deformity or abnormality to coastal organisms, it is essential to quality check of ZPT in PP for environmental safety. However, as far
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Recent Advances in 3D Bioprinted Tumor Microenvironment Biochip J. (IF 2.309) Pub Date : 2020-06-11 Jinseung Bae; Seogkyu Han; Sungsu Park
Tumors in vivo exist in a microenvironment, so-called tumor microenvironment (TME), which includes peripheral blood vessels, immune cells, fibroblasts, signalling molecules and extracellular matrix. In recent years, considerable efforts have been focused on developing bioprinting methods because 3D bioprinting can better recapitulate TMEs by accurately printing different types of TME cells in spatially
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Warfarin Pharmacogenetics: Single-nucleotide Polymorphism Detection using CMOS Photosensor-based Real-time PCR Biochip J. (IF 2.309) Pub Date : 2020-05-19 Seojin Bae; Kukhui Son; Doyoung Lee; Sangeun Han; Kyunghak Choi; Sanghyo Kim
Warfarin is a drug associated with blood clotting. As the side effects of this drug are fatal, it is important to adjust its dosage for each individual. In particular, DNA analysis of patients is necessary because the effect of the drug varies greatly depending on the genotype of the individual. Real-time PCR technology is usually used for DNA analysis. This technology requires expensive equipment
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Separation of Macrophages Using a Dielectrophoresis-Based Microfluidic Device Biochip J. (IF 2.309) Pub Date : 2020-05-08 Ying Zhang; Shangyu Wang; Jie Chen; Fang Yang; Guiying Li
Macrophages are an important component of the immune system and play a key role in tissue damage repair and immune defense. As a potential biomarker, macrophages contribute to the early diagnosis and therapy of cancer and other disease. The isolation of macrophages is a key step in relevant research and clinical applications. However, conventional macrophage separation and purification methods rely
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Separation, Purification, and Detection of cfDNA in a Microfluidic Device Biochip J. (IF 2.309) Pub Date : 2020-05-08 Hyeon Gi Kye; Christian D. Ahrberg; Byeong Seon Park; Jong Min Lee; Bong Geun Chung
Cell free DNA (cfDNA) is degraded DNA fragments found in the blood plasma of cancer patients. While cfDNA is a good marker for early diagnostics and cancer prognosis, the extraction of cfDNA from whole blood and sample preparation for later sequencing is still challenging. Here, we presented a microfluidic device for the removal of cells from a cfDNA sample in a first step. In a second step, carboxylated
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Viscosity Measurement of Whole Blood with Parallel Plate Rheometers Biochip J. (IF 2.309) Pub Date : 2020-05-06 Chunhwa Ihm; Dae-Sik Lee; Kyung Hyun Ahn; Ju Seok Oh
There are many factors that affect the increase or decrease of viscosity of the whole blood. Because of this, measuring the viscosity of whole blood is difficult to correlate with specific etiologies, so it tends to disregard the information on blood viscosity. This study is to find out the characteristics of the method of measuring the viscosity of whole blood using the parallel plate method, which
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Portable Pumpless 3D-Printed Chip for On-Site Colorimetric Screening of Hg 2+ in Lake Water Biochip J. (IF 2.309) Pub Date : 2020-04-16 Ji Won Lim; Tai-Yong Kim; Min-Cheol Lim; Sung-Wook Choi; Min-Ah Woo
The early detection of mercury ions in water sources should be emphasized to prevent human exposure to mercury through drinking water or food consumption. Here, we used a three-dimensional (3D)-printed multiarray chip to achieve colorimetric detection of inorganic mercury ions in environmental matrices such as lake water. The chip designed for the multidetection of inorganic mercury did not require
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Lanthanide-Doped Upconversion Nanomaterials: Recent Advances and Applications Biochip J. (IF 2.309) Pub Date : 2020-03-16 Dongkyu Kang; Eunyoung Jeon; Suyeon Kim; Joonseok Lee
Lanthanide-doped upconversion nanomaterials are well known for converting near-infrared (NIR) excitation photons into visible, ultraviolet, and NIR emission photons. Excitation of NIR light offers low autofluorescence background and high penetration depth in biological environments due to the reduced light scattering. Consequently, these have attracted considerable interest because their exceptional
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Hydrophoresis — A Microfluidic Principle for Directed Particle Migration in Flow Biochip J. (IF 2.309) Pub Date : 2020-03-16 Sungyoung Choi
Despite the stereotype that secondary flow fields induced by surface grooves are effective for microfluidic mixing and increase the entropy of different fluid flows, many efforts have been made to utilize the grooves for particle separation and focusing, decreasing the entropy of particle distribution. As part of these efforts, hydrophoresis has been proposed to define deterministic particle trajectories
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3D Pancreatic Tissue Modeling in vitro : Advances and Prospects Biochip J. (IF 2.309) Pub Date : 2020-03-16 Myungji Kim; Dong Gyu Hwang; Jinah Jang
The pancreas is a relatively small organ, but it has structural and functional complexity that makes it difficult to understand underlying disease mechanisms and determine effective treatments. Thus, there is a great need for more effective tools to study pancreatic disease, and breakthroughs in various fields have contributed to development of in vitro tissue models to understand the pathophysiological
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Nanoelectrokinetic Selective Preconcentration Based on Ion Concentration Polarization Biochip J. (IF 2.309) Pub Date : 2020-03-13 Jihye Choi; Seongho Baek; Hee Chan Kim; Jong-Hee Chae; Youngil Koh; Sang Woo Seo; Hyomin Lee; Sung Jae Kim
Nanoscale electrokinetic phenomenon called ion concentration polarization (ICP) has opened a new era in bio- and chemical-analysis platform due to its high efficiency and easy sample handling. In this review, the most recent advancements of selective preconcentration process using ICP were introduced. The flux balances of charged analytes were theoretically analyzed so that the behavior and the shape
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Real-time Monitoring of Biomarkers in Serum for Early Diagnosis of Target Disease Biochip J. (IF 2.309) Pub Date : 2020-03-13 Dong-Hyung Kim; Sung-Ho Paek; Da-Yeon Choi; Min-Kyung Lee; Ji-Na Park; Hyun-Mo Cho; Se-Hwan Paek
Real-time monitoring of biomarkers has important applications in immediately determining, for example, disease progress or therapeutic effects to clinical decisions in the fields of diagnosis, prognosis and therapy. However, technologies developed in this area require frequent sample drawings, unattended analysis in situ, secure online monitoring, and, above all, patient’s safety, so from this point
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Recent Advances in Anti-inflammatory Strategies for Implantable Biosensors and Medical Implants Biochip J. (IF 2.309) Pub Date : 2020-03-12 Dongwon Lee; Kijun Park; Jungmok Seo
Due to the global aging at an accelerated rate, demands for medical implants have been rapidly increasing. Consequently, a number of complications associated with medical implants has been reported. The complication is mainly due to foreign body response (FBR) which is a nonspecific immune response. Once the implants inserted inside the body, the immune system recognize the implant as foreign body
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Multiscale Biofluidic and Nanobiotechnology Approaches for Treating Sepsis in Extracorporeal Circuits. Biochip J. (IF 2.309) Pub Date : 2020-03-12 Joo H Kang
Infectious diseases and their pandemics periodically attract public interests due to difficulty in treating the patients and the consequent high mortality. Sepsis caused by an imbalanced systemic inflammatory response to infection often leads to organ failure and death. The current therapeutic intervention mainly includes “the sepsis bundles,” antibiotics (antibacterial, antiviral, and antifungal)
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Surface Sensitive Analysis Device using Model Membrane and Challenges for Biosensor-chip Biochip J. (IF 2.309) Pub Date : 2020-03-11 Ji Min Baek; Yong-Sang Ryu
Lipid membranes and their applications in analytical biochip devices represent a great tool for the study of membrane dynamics and the related biological phenomena. Lipid-membrane-assisted surface-sensitive sensors have employed to provide biological information as they improve molecular survivability as well as rule out incorrect signals arising from unwanted nonspecific binding between target molecules
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Advancements in DNA-assisted Immunosensors Biochip J. (IF 2.309) Pub Date : 2020-03-11 Juyoung Kang; Min-Gon Kim
Several types of biosensors have been developed to detect a wide variety of human diseases. Immunosensors are classified as the most representative of all biosensors. They are based on antibodies that selectively recognize specific analytes and have high specificity and sensitivity. However, there are limitations to the types of substances that can be detected, and it is sometimes difficult to achieve
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Colorimetric Determination of Singlet Oxygen Scavengers Using a Protein Photosensitizer Biochip J. (IF 2.309) Pub Date : 2020-03-06 Miso Kim; Eun Hye Kim; Thi Hai Yen Pham; Tuan Anh Hoang Le; Thi Phuong Do; Thi Nga Nguyen; Thi Cuc Nguyen; Ha Phuong Trieu; Thi Thao Do; Young-Pil Kim
Despite the diverse roles of singlet oxygen (1O2) in plants and animals, simple detection of 1O2 has been limited due to technical difficulties. Here we report a colorimetric method for visualizing the generation and suppression of 1O2 using a protein photo-sensitizer, mini singlet oxygen generator (miniSOG). Upon external light irradiation, miniSOG strongly produced 1O2 and oxidized a universal chromogenic
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Low-cost Point-of-Care Biosensors Using Common Electronic Components as Transducers Biochip J. (IF 2.309) Pub Date : 2020-03-05 Yong Duk Han; Hyeong Jin Chun; Hyun C. Yoon
Although vast research has been performed on biosensors, the materialization of low-cost point-of-care (POC) biosensors exhibiting portability, user-friendliness, and highly reliable analytical performance has not yet been achieved. The utilization of electronic/optoelectronic components and devices as signal transducers is being studied as a promising approach for the development of ideal POC biosensors
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Sensitive Colorimetric Detection of Prostate Specific Antigen Using a Peroxidase-Mimicking Anti-PSA Antibody Coated Au Nanoparticle Biochip J. (IF 2.309) Pub Date : 2020-01-30 Xuan-Hung Pham; Eunil Hahm; Kim-Hung Huynh; Byung Sung Son; Hyung-Mo Kim; Bong-Hyun Jun
The use of colorimetric bioassays for protein detection is one of the most promising diagnostic approaches, but their relatively poor detection limits have been a critical issue. In this study, we developed an efficient colorimetric bioassay based on antibody-coated peroxidase-mimicking Au nanoparticles (Au NPs) for the detection of prostate-specific antigen (PSA), which is one of the most widely used
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Hydrogel-integrated Microfluidic Systems for Advanced Stem Cell Engineering Biochip J. (IF 2.309) Pub Date : 2019-12-18 Soohwan An; Seung Yeop Han; Seung-Woo Cho
Previous culture techniques are often ineffective for providing appropriate conditions to cells grown in vitro for efficient growth and maturation. However, the advent of microfluidic chips allows us to manipulate various factors from co-culturing cells to inducing shear stress and biochemical gradient. The above have all been effectively applied to stem cell engineering, allowing dynamic interactions
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A Review of Electrical Impedance Characterization of Cells for Label-Free and Real-Time Assays Biochip J. (IF 2.309) Pub Date : 2019-12-18 Hien T. Ngoc Le; Junsub Kim; Jinsoo Park; Sungbo Cho
The aim of this review is to discuss label-free and real-time assays characterizing the electrical impedance of cells for biomedical and pharmacological applications. Electrical impedance measurements can be used to non-destructively monitor cellular behavior by analyzing the electrical cell-substrate impedance, which is determined by cellular morphology and distribution on the sensing electrode. Cellular
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Clinical Evaluation of a Novel Micro-lancet (ML) for Minimizing Lancing Pain Biochip J. (IF 2.309) Pub Date : 2019-12-18 Yonghao Ma; Chisong Lee; Ghunil Lee; Yonggeun Cho; Sang-Guk Lee; Hyungil Jung
Patients with diabetes are required to lance their fingers daily for self-monitoring their blood glucose level. Patients might be reluctant to use commercial lancets with thick outer diameters due to lancing pain; this results in the worsening of their health. A metallic microneedle is an ideal substitute to the commercial lancet system and offers a minimally invasive and patient friendly approach
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High-Frequency Interdigitated Array Electrode-Based Capacitive Biosensor for Protein Detection Biochip J. (IF 2.309) Pub Date : 2019-12-17 Tuan Vu Quoc; Viet Nguyen Ngoc; Tung Thanh Bui; Chun-Ping Jen; Trinh Chu Duc
This paper reports a study on developing of a protein detection biochip based on interdigitated array electrodes (IDAEs) capacitive immunosensor. The protein after being preconcentrated in a detection region will be selectively captured and detected by the capacitive immunosensor. Using electrical impedance spectroscopy operated at high-frequency in the range of 100 kHz–1 MHz, the capacitance of the
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Clinical Studies of Ci-5, Sol-gel Encapsulated Multiplex Antibody Microarray for Quantitative Fluorometric Detection of Simultaneous Five Different Tumor Antigens Biochip J. (IF 2.309) Pub Date : 2019-12-17 Jae Kong Kim; Hwan-Moon Song; Jong Woo Jun; Hyun Ji Park; Eun-Su Lim; Kyunghwan Lee; SangWook Lee; Soyoun Kim
Here, we developed Sol-gel based antibody microarray platform for quantitative detection of five different cancer biomarkers in clinical sample simultaneously. This kit called Ci-5, Multiplex Tumor Marker Quantitative Kit, is developed based on proprietary SG Cap™ technology. The Ci-5 has 3×5 format of microarray that contains five different antibodies for capturing multi-targets cancer biomarkers
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Fluorometric Detection of Oncogenic EML4-ALK Fusion Gene based on a Graphene Oxide System Biochip J. (IF 2.309) Pub Date : 2019-12-17 Yu Mi Baek; Yeeun Jeong; Dong-Eun Kim
Detection of the fusion gene composed of echinoderm microtubule-associated protein-like 4 (EML4) and anaplastic lymphoma kinase (ALK) is important for diagnosis of lung adenocarcinoma. We developed a simple PCR-based fluorometric method for detecting the EML4-ALK fusion using a fluorescent probe DNA and graphene oxide (GO). The fluorophore-labeled probe DNA complementary to the EML4-ALK fusion junction
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Electrochemical Immunoassay Based on Indium Tin Oxide Activity Toward a Alkaline Phosphatase Biochip J. (IF 2.309) Pub Date : 2019-12-17 Sunga Song; Young Joo Kim; Ik-Soo Shin; Won-Hyo Kim; Kook-Nyung Lee; Woo Kyeong Seong
In vitro diagnostic technologies, used to evaluate disease status at the point-of-care (POC), are of urgent importance to improve diagnostic capabilities in bladder cancer. However, currently available approaches have a limitation because of their lack of detection for early-staged cancer. Herein, we developed an electrochemical immunosensor for the POC diagnosis of bladder cancer in which the enzyme
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Biologically Benign Multi-functional Mesoporous Silica Encapsulated Gold/Silver Nanorods for Anti-bacterial Applications by On-demand Release of Silver Ions Biochip J. (IF 2.309) Pub Date : 2019-12-06 Seokyoung Yoon; Younghwan Chung; Jin Woong Lee; Junhyuck Chang; Jeon Geon Han; Jung Heon Lee
Although silver (Ag)-based nanoparticles (NPs) are frequently used for bactericidal purposes, they have critical issues including excessive release of Ag+, severe oxidation, and cytotoxicity. In this study, we designed a multifunctional, on-demand antibacterial agent by successively encapsulating bimetallic gold/silver nanorods (Ag/AgNRs) with mesoporous silica (mSiO2) shells. Au/AuNRs were synthesized
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Rapid Subtyping and Pathotyping of Avian Influenza Virus using Chip-based RT-PCR Biochip J. (IF 2.309) Pub Date : 2019-11-11 Na young Kwon; Jeong Jin Ahn; Ji-Hoon Kim; Seol young Kim; Ji Ho Lee; Jung-Hoon Kwon; Chang-Seon Song; Seung Yong Hwang
Frequent outbreaks of avian influenza viruses (AIV), which are influenza A viruses, result in heavy economic and national damages. However, current diagnostic methods require about 5–7 days for AIV to be confirmed, which allows enough time for the widespread dissemination of the virus. Therefore, there is a need for rapid and accurate diagnosis of AIV. Here, we developed a diagnostic method using chip-based
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A Simple and Multiplex Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of SARS-CoV Biochip J. (IF 2.309) Pub Date : 2019-11-11 Jin Hwa Kim; Minhee Kang; Eunkyoung Park; Doo Ryeon Chung; Jiyeon Kim; Eung Soo Hwang
The current diagnosis of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) relies on laboratory-based tests since its clinical features are nonspecific, unlike other respiratory pathogens. Therefore, the development of a rapid and simple method for on-site detection of SARS-CoV is crucial for the identification and prevention of future SARS outbreaks. In this study, a simple colorimetric
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A Simple Liquid Crystal-based Aptasensor Using a Hairpin-shaped Aptamer for the Bare-Eye Detection of Carcinoembryonic Antigen Biochip J. (IF 2.309) Pub Date : 2019-11-11 Huihui Ren; Chang-Hyun Jang
Sensitive detection of carcinoembryonic antigen (CEA) is highly important in clinical diagnosis because CEA is an effective tumor marker associated with gastric, lung, rectal, and breast cancers. Thus, the development of an accurate, simple, and efficient method for the detection of CEA remains a long-term clinical goal. In this study, a liquid crystal-based aptasensor was constructed for the labelfree
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A Matrix Metalloproteinase Sensing Biosensor for the Evaluation of Chronic Wounds Biochip J. (IF 2.309) Pub Date : 2019-10-17 So Min Kang; Hana Cho; Dasom Jeon; Seong Hyuk Park; Dong-Sik Shin; Chan Yeong Heo
The increasing prevalence of the elderly in global society is being accompanied by rapidly increasing numbers of patients with cerebrovascular disease or diabetes, and patients with chronic wounds, including complications of ulcers and diabetic foot ulcers. Many studies have sought to develop wound sensors based on various targets. However, there is no proper device for in situ detection that uses
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Electrochemiluminescent Chemosensors for Clinical Applications: A Review Biochip J. (IF 2.309) Pub Date : 2019-09-16 Cuong Khac Phu Truong; Thao Dang Duy Nguyen; Ik-Soo Shin
Economic development has raised concerns about human healthcare and disease prevention from its early stages. In that regard, the detection of biomarkers is crucial for early diagnosis of diseases, and it is an essential tool for managing various health conditions. The clinical diagnostics industry is worth hundreds of billions of dollars and has been expanding. However, the traditional methods for
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A Novel Paper-plastic Microfluidic Hybrid Chip Integrated with a Lateral Flow Immunoassay for Dengue Nonstructural Protein 1 Antigen Detection Biochip J. (IF 2.309) Pub Date : 2019-09-11 Ma. Kristine Yuzon; Jae-Heon Kim; Sanghyo Kim
This study presents an easy paper-plastic hybrid microfluidic chip fabrication method that explored the use of oven baking a wax-printed pattern for microfluidic channel assembly combined with the use of a double-sided adhesive tape for layer binding. More specifically, the method includes printing of a wax pattern onto the CA film & oven baking of the pattern along with the backed NC membrane at 120°C
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A Multichannel Electroosmotic Flow Pump Using Liquid Metal Electrodes Biochip J. (IF 2.309) Pub Date : 2019-08-29 Yongchang Zheng; Kai Kang; Fucun Xie; Hanyu Li; Meng Gao
Injecting a room-temperature liquid metal into microchannels offers a simple, rapid, and low-cost method of fabricating microfluidic electrodes. In this work, these electrodes are used to develop a multichannel electroosmotic flow pump for high-flow-rate microfluidic bio analysis applications. In this pump, two identical square-wave shaped liquid metal electrodes were located at both ends of pumping
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Paper-based Molecular Diagnostics for the Amplification and Detection of Pathogenic Bacteria from Human Whole Blood and Milk Without a Sample Preparation Step Biochip J. (IF 2.309) Pub Date : 2019-08-09 Jee Won Lee; Van Dan Nguyen; Tae Seok Seo
We have developed a novel paper-based molecular pathogen diagnostic method by combining the direct loop-mediated isothermal amplification (Direct LAMP) in a paper reactor and an immunochromatographic strip (ICS) detection without any DNA preparation step. The mineral paper is durable, oil- and tear-resistant, and waterproof, so it is a proper material for point-of-care (POC) testing owing to facile
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Generation of 3D Microparticles in Microchannels with Non-rectangular Cross Sections Biochip J. (IF 2.309) Pub Date : 2019-07-26 Sung Min Nam; Kibeom Kim; Il-Suk Kang; Wook Park; Wonhee Lee
Flow lithography in a conventional rectangular microchannel is limited to fabrication of particles for which only the shapes of top perimeters are controlled. We present a flow lithography technique for fabrication of microparticles of diverse 3D shapes and multiple layers using non-rectangular microchannels with designed cross sections that allow the creation of complex shapes and diverse cross-sectional
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Multifunctional Nanomaterial-alginate Drug Delivery and Imaging System for Cancer Therapy Biochip J. (IF 2.309) Pub Date : 2019-07-24 Seokyoung Yoon; Junhyuck Chang; Nayoung Kwon; Seokhoon Moon; Youngmin Park; Kwon Hoon Han; Byungkwon Lim; Jung Heon Lee
Although chemotherapy is mostly performed by direct injection of the chemotherapeutic agents systemically, this approach can cause undesirable side effects in normal tissues and lacks targeting efficiency. In this study, we have developed a micron-sized, bead-type multifunctional anticancer-drug carrier that can be injected in the vicinity of a lesion using a syringe. The multifunctional anticancer-drug
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Molecular Docking-assisted Protein Chip Screening of Inhibitors for Bcl-2 Family Protein-protein Interaction to Discover Anticancer Agents by Fragment-based Approach Biochip J. (IF 2.309) Pub Date : 2019-07-22 Myoung-Schook Yoou; Sungjoon Cho; Youngjin Choi
For fragment-based cancer drug discovery, we introduced a molecular docking simulation combined with a protein chip assay. Protein chip technology was used to find fragment-hits that had inhibitory activity against Bcl-2 protein from 131 pre-selected fragment chemicals. Molecular docking simulation was performed for the 12 identified fragment-hits to establish the binding mode of these compounds in
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