A key trait impacting the maturity of qingke is the heading date. Identification of major stable quantitative trait loci (QTL) for heading date trait is important for maturity improvement in qingke. Therefore, we conducted QTL mapping of an F2 population of 146 lines and constructed a high-density linkage map with 1,243 recombination bin markers. After phenotyping the F2:3 across three environments

(2021). Correction. All Life: Vol. 14, No. 1, pp. 881-881.

Novel COVID-19 is the most considerable health threat the humanity has faced in decades, with global impact also in the social and economic scopes. Moreover, SARS-CoV-2 involves an unprecedented exciting scientific challenge that has focused all efforts on defeating the new coronavirus. Research results are continuously increasing and updating knowledge about the virus and the disease, and understanding

BMPR2 encodes the bone morphogenetic protein receptor type 2. Most of heritable pulmonary arterial hypertension is caused by mutations of BMPR2. Pulmonary arterial hypertension is characterized by increased pulmonary vascular resistance and sustained elevation of mean pulmonary arterial pressure. Here we sought to identify novel mutations in a family with pulmonary arterial hypertension. Whole-exome

Alu elements, the most abundant retrotransposed elements in the human genome, are commonly located in introns and affect alternative splicing. We previously showed that an intronic antisense Alu element enhanced alternative splicing in a minigene model. The RNA splicing experiment was performed using a minigene consisting of exons 9–11 of ACAT1 with an antisense AluSx inserted into intron 10 in a pCAGGS

Muscle-derived stem/progenitor cells (MDSPCs) have been demonstrated as a promising source of cellular therapy for the regeneration of peripheral nerves and how to improve the therapeutic potential of these cells is the focus of future research. This study aims to investigate the role of hypoxia on functional effects in MDSPCs. In this study, MDSPCs were isolated from skeletal muscles of limbs; meanwhile

To investigate the effect of astragalus heart-protecting decoction on rats with dilated cardiomyopathy (DCM). 36 healthy rats were divided into normal control group (n = 12, unmodeled rats without receiving decoction), DCM + control group (n = 12, modeled rats without decoction), and DCM + treatment group (n = 12, modeled rats receiving decoction). Outcomes of the three groups were compared. Serum

Interstitial lung disease (ILD) presents as progressive scarring of lung tissue, which may affect respiratory function and restrict oxygen-exchange into the bloodstream, finally leading to dyspnea, dry cough and decreased quality of life. Increasing evidence indicates that genetic factors may be crucial in the development of ILD. At present, the main pathogenic genes of ILD include genes vital for

To determine association of severe anemia and red blood cell (RBC) transfusions with bronchopulmonary dysplasia (BPD) in preterm infants with gestational age＜32 weeks, a retrospective analysis was conducted on 364 newborns from August 1,2018, until August 31,2020. 135 patients were diagnosed with BPD and the other 229 neonates were included in the non-BPD group. One-to-one propensity score matching

The preclinical studies on circRNA are the most popular topic these days, including many latest reports about diabetic retinophathy (DR) for both treatment and diagnosis. This review aims to investigate the treatment effects of circRNAs on DR, its potential mechanism and cell targeting in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines, and finally

Brucella abortus is highly contagious and can result in abortion or infertility in animals. This study investigated the mechanism of B. abortus-induced mucosal immunity at different points after infection in a mouse model. GSE125765 was obtained from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) in nasal-associated lymphoid tissues at 1, 6, and 12 h after intranasal immunization

Several purple-leaf tea has been utilized around the world. Flavonoids accumulation is one of the reasons why purple-leaf tea has unique pharmacological value and flavor. Although abundant researches have focused on identification the key genes associated with flavonoids biosynthesis in purple-leaf tea cultivars, However, the accumulation pattern of flavonoids in purple-leaf tea is still worth investigation

Stemness is now an accepted cause of treatment failure and relapse in cancer patients. Laryngeal squamous cell carcinoma patients face a high recurrence and metastatic disease. Research efforts have focused in recent years on discovering biomarkers of poor prognosis in laryngeal cancer patients. SALL4 is an embryonic stem cell factor which has been reported to be highly expressed; and to play a role

Background: Enhancer RNA (eRNA) is a non-coding RNA transcribed by enhancers and has a crucial role in controlling gene transcription. However, the potential functions of eRNA in prostate cancer (PCa) are still not fully understood. Methods: Clinical information and expression data of eRNAs and their target genes for PCa were downloaded from TCGA. Each eRNA in PCa samples was divided into high- and

Researches on drug delivery system (DDS) have made incredible progress, but there are still problems such as insufficient targeting and undesirable treatment efficiency. To improve the above problems, a stem cell-based DDS was developed. Stem cell and its derivatives (cell membranes, extracellular vesicles/exosomes) have been used as important drug delivery vehicles in the treatment of a number of

Coleus aromaticus is a herbal medicine that has been referred for its medicinal properties in traditional, ayurvedic, and folklore medicines. Therefore, various research in the area for biochemical characterization of its biochemical compounds are extensively carried out using various techniques including spectroscopy and chromatography analysis. The list of bioactivities reported for this plant includes

Folic acid and methylenetetrahydrofolate reductase enzyme (MTHFR) gene C677T (rs1801133) SNP have been proofed to influence the spermatogenesis. This study aimed to investigate the biochemical, histological, and immunohistochemical (IHC) of adult male rabbits after induction of infertility by adenine, and the role of MTHFR C677T genotypes with or without folic acid therapy. Thirty adult male rabbits

The liver, an organ with robust regenerative capacity, is involved in many physiological functions, such as detoxification and modulation of optimal brain function. Exosomes are secreted by almost all cell types and mediate intercellular communication, as well as, they are involved in neuroinflammatory process. In this study, we found that fetal liver cell-derived exosomes (FLC-EXOs) administration

This study aimed to explore the potential molecular mechanisms of FuZheng XiaoJi prescription (FZXJP) on Colorectal Cancer (CRC) cells. Transcriptome sequencing was performed on HT-29 cells treated with FZXJP and not followed by selecting differentially expressed genes (DEGs). Functional enrichment analysis and protein-protein interaction (PPI) analysis were performed for the DEGs. The transcription

Pituitary metastasis is a rare situation in clinical practice and has been estimated to take up no more than 1% of tumors found in sellar region, in which the incidence of a secondary metastatic lesion to the sella by hepatocellular carcinoma (HCC) is extremely rare, only 15 cases of which has been reported yet. The aim of this study is to share the detailed procedures and clinical strategies of this

ABSTRACT Spermatogenesis process plays a major role in male fertility. Quercetin, a flavonoid, is found in daily food or traditional Chinese medicine, which has a significant effect on treating inflammatory diseases. Several studies have shown that quercetin can improve male spermatogenesis. However, the intrinsic mechanism has not been systematically investigated. The network pharmacology approaches

Cell cycle inhibitors are considered as hallmark strategy for cancer treatment due to their relatively higher selectivity and efficacy on various cancer types in comparison to cytotoxic agents. Small molecules target dividing cells in G1/S, G2 or M phases of cells to arrest and eventually trigger cancer cells to enter apoptosis and/or inhibit tumor growth. Cell cycle arrest at G2/M phase is a widely

In the current study, a model of ‘fungus-root’ interaction was designed using host Zea mays inoculated with a metal-tolerant dark septate endophyte (DSE) strain of Exophiala pisciphila H93 both under nutritional- (Zn0) and zinc-stressed (Zn550) conditions. We found that the co-cultured maize benefited from the alleviated excessive Zn toxicity by its root-associated E. pisciphila, through significantly

The purpose of this study was to determine the potentiality of the composting process and biological pretreatment of using P. florida to improve the nutritive value and enhance the digestibility of rice straw. A total of 40 rice straw samples with and without composting treated with P. florida at different substrate harvesting periods (full ramification, 1st, 2nd, and 3rd flushing) were evaluated by

Ovarian cancer (OC) is a common gynecologic malignancy worldwide. Several members of the tripartite motif (TRIM) family are of great importance in various human malignancies. In this study, we aimed to figure out the function and mechanism of TRIM52 in OC. Western blot and RT–PCR were used to examine the expression level of TRIM52. Cell counting kit-8 (CCK-8) assay was used to measure cell proliferation

Bladder cancer (BCa) is the most common urological tumor, and most BCas are diagnosed at an advanced stage due to the lack of reliable diagnostic markers. In this study, transcriptomic and clinical data from BCa patients in the TCGA database were analyzed and all patients were randomly divided into a training group and a validation group. Univariate Cox regression analysis was performed to screen miRNAs

Acetyl xylan esterases are among the key enzymes in the xylan degradation enzyme system. However, acetyl xylan esterases from natural microorganisms have low expression and low enzyme activity and are impure. In this study, a new xylanase gene, est1051, from the metagenomic library, was expressed in the prokaryotic system. Its enzymatic properties were explored, including optimum temperature and pH

Chinese mitten crab, Eriocheir sinensis (Varunidae), is one of the most popular and widely cultivated freshwater crab species in the Chinese food industry, with high commercial importance and nutritional value. We analyzed the diversity of culturable fungi in the gut of E. sinensis collected from a rice-crab co-culture system in Tianjin, China. We isolated 41 fungal strains from the gut of analyzed

Low molecular weight organic acids (LMWOAs) are common and abundant compounds in the environment, responsible for the release of the nutrients from rhizosphere into the soil solution and, therefore, facilitating the availability of these nutrients for plants. Sewage sludge biochar (SSB) is considered to be a potential source of phosphorus (P) in soil. In this context, the efficacy of twelve LMWOAs

ABSTRACT The molecular identification of different taxa of genus Acacia plays a critical role to understand the evolutionary history of this important plant that has many medicinal and ecological benefits and distributed in many regions around the world (Africa, Asia, Australia and the Americas). This is the first study that used DNA barcoding (rbcL and matK) with 24 taxa of genus Acacia collected

Gender research shows excellent significance in the breeding and production of poultry, achieving the actual needs of poultry through targeted gender regulation could increase economic benefits. However, the mechanism of sex determination in poultry remains unclear. We respectively constructed HMGCS1 overexpression and interference vectors for lentivirus packaging. Vascular microinjection was performed

‘Huabai 1’ is a novel albino tea germplasm that has a stable albino phenotype and prolonged albino period. In this study, widely targeted metabolomics was employed to analyze the metabolic profile of ‘Huabai 1’, and compare metabolites between albino shoots of ‘Huabai 1’ (HBW) and albino shoots of the broadest cultivated cultivar ‘Baiye 1’ (BYW). A total of 359 differential metabolites were identified

Accumulation of amyloid-β (Aβ) is implicated in the pathogenesis and development of Alzheimer's disease (AD) and it is a prime suspect for causing neuronal loss and cognitive deficits during AD. To explore the mechanisms of Aβ-induced neurodegeneration, we used Aβ-treated primary cortical neuron culture as a cell model of AD and investigated the function of miR-137 in this process. qRT-PCR and western

Competitive endogenous RNA (ceRNA) plays a vital role in cancer stem cells. In the present study, we aimed to investigate the role of the circular RNA (circRNA) antisense to the cerebellar degeneration-related protein 1 transcript (CDR1as) during competitive inhibition of microRNA-7 (miR-7) in the regulation of autophagy to modulate the proliferation and migration of breast cancer stem cells. The CD44+/CD24-

Microbial fuel cell (MFC) has novel technological advances in simultaneous power generation and wastewater treatment applications. In this study, low-cost biosynthesized α-MnO2 nanoparticles integration with conducting polyaniline (PANI) matrix to form α-MnO2/PANI hybrid nanocomposite was fabricated by in situ polymerization method. The prepared material was characterized through UV-Vis spectroscopy

Cirrhosis is the primary driver of hepatocellular carcinoma (HCC). Blocking the deterioration of cirrhosis into HCC would be of benefit for long–term survival. We applied a series of bioinformatic online databases to select and analyze the hub genes concerning cirrhosis and HCC, and identified UBE2T and CYP3A4 as hub genes for cirrhosis-HCC transformation. An elevated UBE2T and a decreased CYP3A4 were

Inflammation is a defense mechanism that the immune system uses in response to harmful stimuli such as pathogens, damaged cells, toxic compounds, or irradiation. These stimuli may induce inflammatory response and potentially tissue damage in respiratory, cardiovascular, digestive, nervous, endocrine, urinary, or reproductive systems. Inflammatory diseases include a broad array of disorders and conditions

Mushrooms are a nutritionally, economically, and biotechnologically valuable group of organisms. They are packed with nutritional value in the form of low calories, high protein, and fiber contents. Besides being an important source of food, mushrooms are exploited by man in various ways. Mushrooms can be used in the pharmaceutical industry as antioxidant, anticancer, and immunomodulating agents. They

This study investigates the curative response of ethanol extract of Cassia sieberiana leaves (EECSL) on testosterone-induced benign prostatic hyperplasia (BPH). Thirty rats of 11–12 weeks old weighing 120–160 g were grouped into six of five rats each. Group 1 was the normal control; groups 2–6 were induced with testosterone (5 mg/kg body weight) for 21 days following standard procedures. Group 2 serves

Heptaphylline, a carbazole alkaloid, has been shown to exert anticancer effects against different human cancers. The study was designed to evaluate the anticancer effects of heptaphylline against the human pancreatic cancer cell and to explore the underlying mechanism. The results showed that heptaphylline inhibited the proliferation of all the pancreatic cancer cell lines with lowest IC50 of 12 µM

The present study investigated the effect of Trigonella foenum-graecum extract (TFGE) on GH3 and HEK293 pituitary adenoma cell growth in vitro in mice model in vivo and evaluated the underlying mechanism. TFGE exposure in dose-dependent manner suppressed the viability of GH3 and HP75 cells at 5–30 µM concentrations. Treatment of GH3 and HP75 cells with 30 µM TFGE inhibited colony formation significantly

To assess the state of coenopopulations of rare and endemic plants, it is important to evaluate the age index, efficiency index, and delta-omega analysis. For this, we studied the age composition of six coenopopulations of Lipskya insignis (Apiaceae) located in the south of the Republic of Uzbekistan. The results showed that in all studied coenopopulations generative individuals prevail, the number

Cisplatin (CISP) is one of the most commonly used drug for treating various cancers and solid tumours, however, its usage has been heavy restricted due to its deleterious side effects including peripheral neuropathy. Therefore, this study describes the anti-hyperalgesia and anti-allodynia effects of Tiliacora triandra (TTE) in CISP induced peripheral neuropathy. Peripheral neuropathy was established

Steroidal glycoalkaloids (SGAs) existing in most potato tissues are toxic to humans when the fresh weight is over 200 mg kg−1, and high SGAs content would also damage the quality of potato tubers. Sterol side chain reductase 2 (StSSR2) is a key enzyme in the biosynthetic of SGAs in potatoes. To reduce the concentration of SGAs in tetraploid cultivated potato (Solanum tuberosum L.), the gene StSSR2

In order to establish nomograms that could forecast the postoperative survival for patients with appendiceal cancer after surgery, this study collected 5945 patients with surgically removed appendiceal cancer from the Surveillance, Epidemiology, and End Results database. Overall survival (OS) and cancer-specific survival (CSS) were analyzed by Cox regression analysis and nomograms. The population was

Lupinus is one of the most geographically widespread genera. It can be divided into New World and Old World Lupinus species according to the geographical distribution of its members. However, there are relatively few studies on their genetic differences, especially regarding the taxonomy and geographical evolutionary history of New World species. Here, we conducted comparative, phylogenetic, and evolution

Aloperine has been shown to exhibit tremendous pharmacological potential. The present study was designed to investigate the effects of aloperine against human liver cancer cells and to explore the underlying mechanism. The results showed significant (P < .05) upregulation of GROα (GRO1 oncogene) in liver cancer tissues and cell lines. However, the expression of GROα significantly (P < .05) declined

We investigated the expression and significance of N-terminal molecular fragments of osteocalcin (N-MID), plasminogen activator inhibitor-1 (PAI-1), and RNA-binding motif 5 (RBM5) in brain metastases of lung cancer. Totally, 156 patients with lung cancer were enrolled as the research objects, of which 120 patients without brain metastases were allocated to group A, and 36 patients with brain metastases

This study aimed to investigate the effect of the miR-29c/PTEN axis on the PI3 K/Akt/NF-kB pathway in rats with severe pneumonia (SP). A total of 20 Sprague–Dawley rats were selected, of which 10 were randomly selected for modeling SP and included in the model group (MG) and the other 10 were included in the control group (CG). Expression levels of miR-29c and PTEN were measured in the peripheral blood

A communal disease of the breast is breast cancer which happens mainly in women. Breast cancer is the most common cancer in women in both the developed and less developed world. The potential biological aggressiveness and behavior of breast neoplasm are of prognostic significance in tumor pathology and this can be assessed by measuring the tumor proliferative activity and malignant transformation.

Harmful algal blooms caused by cyanobacteria have detrimental impacts on lake ecosystems. Uncovering the factors influencing such blooms could facilitate aquatic ecosystem conservation and early warning activities. The experiment simulated the environmental characteristics of shallow eutrophic Lake Ulansuhai in arid regions of China. The experiments were conducted at various temperatures (6, 10, 19

We explored the therapeutic effects of liraglutide combined with metformin and Diamicron in elderly patients with type 2 diabetes mellitus (T2DM) and the effects of this regimen on glucose – lipid metabolism and islet β-cell function. Ninety-two elderly patients with T2DM admitted to our hospital were selected as research participants. Of these, 42 patients treated with metformin and Diamicron were

Among abiotic stresses, drought influences plant growth and productivity, and plant stress responses depend on stress intensity and genotype. The effects of simulated drought stress on germination parameters were tested for tolerance and sensitivity in 59 barley accessions from all regions of Iraq and phenotypic, physiological, and biochemical responses associated with drought stress were investigated

Intracellular signaling complexes of the innate immune system orchestrate the development of inflammation. Porphyromonas gingivalis is a commensal bacterium that induces periodontal pyroptosis. The transcription factor Dec1 can regulate the expression of inflammatory molecules. In this study, we investigated whether a deficiency of Dec1 activates/represses Caspase-1 and/or Gasdermin-D in periodontal

Metastasis is one of the most significant independent risk factors that can negatively affect prostate cancer (PCa) patients. However, the exact mechanisms have not been fully elucidated. To illustrate the mechanisms underlying PCa metastasis, we conducted a series of integrated bioinformatics analyses. The essential genes involved in PCa metastasis were obtained by analyzing differentially expressed

Dexamethasone administered by autologous red blood cells (RBCs) is used in clinical trials and as compassionate therapy to alleviate ataxia telangiectasia (AT) symptoms, providing long-term delivery of low doses of the drug. In the present paper, we report a dexamethasone-induced variation in gene expression that differed between healthy and AT primary fibroblasts simulating patient conditions. Gene

Sepsis, a life-threatening organ dysfunction, continues to cause considerable morbidity. Reliable animal models to evaluate therapeutic options are urgently required, however, some of the most established ones are technically challenging. We herein present a murine model of abdominal sepsis based on the intraperitoneal (i.p.) injection of a defined inoculum of stool specimens (IPSI). Fecal bacterial

Microalgae are increasingly being preferred as sustainable biomass feedstock for biofuels production due to their high growth rate and lipid yield. However, optimal production requires the use of economical native microalgal species which are well adapted to local climatic and ecological conditions. Therefore, this study’s main objective was to isolate and select local microalgal species for prospective

Studies have reported frequent downregulation of microRNA-125-5p (miR-125) in osteosarcoma tissues. However, the molecular role and mechanism of action of miR-125-5p in osteosarcoma via regulation of Kruppel-like factor 13 (KLF13) is yet to be explored. The results of the present study miR-125-5p are significantly (P < .05) downregulated in human osteosarcoma tissues and cell lines. Overexpression

Human epidermal growth factor receptor 2 (HER2) proteins are overexpressed in a certain proportion of gastric cancer cases. Trastuzumab is a humanized monoclonal antibody that targets the HER2 receptor. Previous studies have demonstrated a synergistic interaction between trastuzumab and 5-fluorouracil/cisplatin in HER2-positive gastric cancer cells. However, it is unclear whether it is rational to