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Cell-free systems for a multi-pronged approach to next-generation therapeutics and diagnostics Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-03-17
Abstract Effective diagnostics and therapeutics are foundational to modern medical technology. As the drug modality transitions from traditional small-molecule drugs to more intricate protein and nucleic acid-based therapies, there is mounting momentum towards personalized therapeutic approaches. These evolving paradigms challenge conventional biomanufacturing practices, highlighting the need for agile
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Development and evaluation of orbital rocking motion-based single-use cell culture system, the CELBIC® system Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-03-16 Hyunwoo Kim, Seohyun Park, Rock Ki Kim, Yeong Ok Baik, Kyung Nam Kim, Duk Jae Oh
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Establishment of an optimized electroporation method for Halomonas sp. YK44 and its application in the coproduction of PHB and isobutanol Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-03-15
Abstract Many Halomonas spp. thrive in high-salinity environments, and their resistance to high salt levels allows for their cultivation in non-sterile conditions. Despite their robustness and potential poly (3-hydroxybutyrate) (PHB) production capability, there are relatively few reports on the engineering of various Halomonas species, and there are still some difficulties in genetically engineering
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Photothermal heating of cell-free reactions for on-site production of recombinant proteins Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-03-14 Kyunghwan Yeom, Yu Jin Park, Hansol Kim, Dong-Yeon Song, Dong-Myung Kim, Ji-Ho Park
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In silico screening and validation of different dehydrogenases to produce 2,3-butanediol in Bacillus subtilis Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-03-13 Sailee Sanjay Asolkar, M. Anju, Ravindra Kumar, Apoorva Deshmukh, Anand Ghosalkar, Pramod Kumbhar
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Use of antioxidants to extend the storage of lyophilized cell-free synthesis system Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-03-13 Kyu Jae Kim, So Jeong Lee, Dong-Myung Kim
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Efficient production of 1,3-propanediol from glycerol by a newly isolated soil bacterium using fed-batch fermentation Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-03-12 Selim Ashoor, Zhuang Yao, Chan Woo Song, Haeng Lim Lee, Hyeon Jeong Seong, Sampathkumar Palaniswamy, Jong Myoung Park, Hyohak Song, Yu-Sin Jang
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Engineering potyvirus-like particles to display multiple copies of tuberculosis antigens Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-03-11 R. Princess, M. L. Stephen Raj
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Bioanalysis of ursodeoxycholic acid and its metabolites and improved oral bioavailability using mixed micelles with poloxamer 407 and polysorbate 80 Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-03-11 So Yeon Jeon, Chul Haeng Lee, Jihoon Lee, Ji-Hyeon Jeon, Yelim Jin, Im-Sook Song, Min-Koo Choi
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Reinforced levan-based electrospun nanofibers for application as adhesive scaffolds for tissue engineering Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-03-09 Young Hoon Song, Jeong Hyun Seo
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Microbial production of dextran using pineapple waste extract: a two-step statistical optimization of submerged fermentation conditions and structural characterization Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-03-04 Ashutosh Tripathy, Mukesh Kumar Patel, Snehasis Chakraborty
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Catechol- and thiol-containing binder that aggregates granular xenografts in reconstructed bone defects by mimicking mussel wet adhesion Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-02-22
Abstract Xenograft bone granules are bone graft materials that are widely used to reconstruct bone tissue in clinical practice, and their clinical success requires aggregation with a biocompatible binder. While the catechol mussel bioadhesive has been used as a key universal biomedical glue moiety, the thiols in mussel adhesive proteins have recently been shown to play key roles in mussel wet adhesion
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Targeted siRNA delivery to lung epithelia reduces airway inflammation in a mouse model of allergic asthma Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-02-21 Irfan Ullah, Hyo Sung Choi, Changseon Choi, Kunho Chung, Jae Wook Jung, Gyeongju Yun, Seoyoun Heo, Yujong Yi, Eunhwa Kang, Sang-Heon Kim, Ho Joo Yoon, Taiyoun Rhim, Sang-Kyung Lee
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Enhancing transduction efficiency of adeno-associated virus 9 by cell line engineering: implication for gene therapy potency assay Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-02-19
Abstract Adeno-associated virus (AAV)-mediated gene therapy holds significant promises to treat or potentially cure various human diseases. Although AAV holds promise for their significant therapeutic potential, batch-to-batch differences can exist from manufacturing; and therefore, a potency assay is required for clinical development of AAV. Among different serotypes, due to its ability to cross blood–brain
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Development and optimization of a modular two-fragment LacI switch for enhanced biosensor applications Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-02-19
Abstract Being able to perform modular design of artificial transcription factors is useful in bioengineering and synthetic biology, particularly in the development of biosensors and therapeutics. This study aimed to develop a two-fragment transcription factor system by splitting a lactose repressor (LacI). To fragment LacI, we screened potential split positions from transposon-based insertional libraries
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Vibrio species as next-generation chassis for accelerated synthetic biology Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-02-19 Changhwan Hong, Yoojin Kim, Hyunjin Lee, Saebom Yun, Hyun Gyu Lim, Jina Yang, Sungho Jang
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A comparison of metabolic engineering strategies applied in Yarrowia lipolytica for β-carotene production Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-02-18 Redife Aslıhan Uçar, Furkan Demirgül, Ömer Şimşek, Hüseyin Erten
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Microfluidics-driven high-throughput phenotyping and screening in synthetic biology: from single cells to cell-free systems Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-02-16 Taeok Kim, Minji Ko, Eugene Rha, Haseong Kim, Hyewon Lee
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Metabolic engineering of erythritol production from glycerol by Yarrowia lipolytica Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-02-16
Abstract Erythritol as a four-carbon polyol has been widely used in food, pharmaceutical and daily chemical industries with characteristics of low caloric value and high chemical stability. Here, a system metabolic engineering strategy was used to increase the yield of erythritol from glycerol in Yarrowia lipolytica by enhancing the substrate transformation and restricting the by-product synthesis
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Enzymatic synthesis of nylon precursors by 4-aminobutyrate aminotransferase and 6-oxohexanoate dehydrogenase Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-02-15 Hoe-Suk Lee, Yung-Hun Yang, Young Joo Yeon, Hyun June Park
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Green synthesis of gold nanoparticles using Acorus calamus leaf extract and study on their anti-alzheimer potential Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-02-16 Haixia Peng, Shuzhen Zhang, Qiaolian Chai, Zhongchang Hua
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Comprehensive study on the inhibition mechanism of alpha-glucosidase by flavonoids via kinetic and structural analysis Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-02-15 Jin Young Lee, Hoe-Suk Lee, Yu-Young Lee, Mi-Hyang Kim, Hyun-Joo Kim, Narae Han, Moon Seok Kang, Young Joo Yeon
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Galactose-based biohydrogen production from seaweed biomass by novel strain Clostridium sp. JH03 from anaerobic digester sludge Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-02-15 Jeong Hyeon Hwang, Hyun Joong Kim, Hyun Jin Kim, Nara Shin, Suk Jin Oh, Jeong-Hoon Park, Won-Dong Cho, Jungoh Ahn, Shashi Kant Bhatia, Yung-Hun Yang
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Natural products ameliorating the adverse health effects by air particulate matter Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-02-14 Dongyup Hahn, Min Jeong Kim, Yun Kwon, Eunjeong Kim, Dong Ho Park, Jong-Sup Bae
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Component analysis and utilization strategy of brown macroalgae as promising feedstock for sugar platform-based marine biorefinery Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-02-14 Jeongho Lee, Hyeonmi Shin, Kang Hyun Lee, Hyeseon Lee, Giwon Lee, Sungho Jang, Gyoo Yeol Jung, Hah Young Yoo, Chulhwan Park
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Development of Tat-fused drug binding protein to improve anti-cancer effect of mammalian target of rapamycin inhibitors Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-02-13 Su Yeon Lim, Sugyeong Kim, Hongbin Kim, Hyun-Ouk Kim, Suk-Jin Ha, Kwang Suk Lim
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Assessment of pulsed dielectrophoretic-field flow fractionation separation coupled with fibre-optic detection on a lab-on-chip as a technique to separate similar bacteria cells Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-02-13 Mohd Firdaus Kamuri, Zurina Zainal Abidin, Mohd Hanif Yaacob, Mohd Nizar Hamidon
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Biochemical, structural characterization and assessing the biological effects of cinnamon nanoparticles Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-02-13 Zahra Sabouri, Neda Shakour, Mohammad Sabouri, Samaneh Sadat Tabrizi Hafez Moghaddas, Majid Darroudi
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Reconstruction of genome-scale metabolic models of non-conventional yeasts: current state, challenges, and perspectives Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-02-13 Eduardo Luís Menezes de Almeida, Eduard J. Kerkhoven, Wendel Batista da Silveira
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Strategies for the enhancement of anti-cancer effect of phosphodiesterase type 5 inhibitors using drug binding fusion proteins Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-02-13 Hongbin Kim, Chang Yeop Keum, Su Yeon Lim, Kwang Suk Lim
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Colorimetric detection of milk spoilage at low temperatures: a novel PDA/ZnO@NC membrane for pH-based freshness monitoring Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-02-13 Zhaowei Li, Huisoo Jang, Sungjin Park, Sun Min Kim, Tae-Joon Jeon
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Fabrication of folic acid–cysteamine-modified silver nanoparticles as promising contrast agent for computed tomography imaging Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-02-13 Wei Lian, Min Gan
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Effects of a novel bioprocess for the cultivation Synechococcus nidulans on Mars on its biochemical composition: focus on the lipidome Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-02-12 Mattia Casula, Giacomo Fais, Agnieszka Sidorowicz, Pierluigi Caboni, Giacomo Cao, Alessandro Concas
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Xylanase enzyme from novel strain and its immobilization onto metal organic framework MOF for fruit juice clarification Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-02-12 Jyoti Kaushal, Madhu Khatri, Gursharan Singh, Shailendra Kumar Arya
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Enhanced fermentative γ-aminobutyric acid production by a metabolic engineered Corynebacterium glutamicum Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2024-02-12
Abstract γ-Aminobutyric acid (GABA) is a non-proteinogenic amino acid with important physiological functions, which has been widely used in food, pharmaceuticals, and polyamides production. The fermentative GABA production by Corynebacterium glutamicum was recognized as one of the most promising methods. However, the problems of low catalytic activity of the heterologously expressed glutamate decarboxylase
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Biofoundries: Bridging Automation and Biomanufacturing in Synthetic Biology Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-12-11 Dae-Hee Lee, Haseong Kim, Bong-Hyun Sung, Byung Kwan Cho, Seung-Goo Lee
Biofoundries represent advanced automation facilities pivotal for streamlining the Design-Build-Test-Learn (DBTL) paradigm in biomanufacturing and synthetic biology, suitable for both academic research and industrial applications. Nonetheless, establishing such a platform demands significant financial and temporal resources while maintaining a forward-looking perspective on automation, equipment compatibility
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Improvement of Lipid and Terpenoid Yield in Thraustochytrids Using Chemical Regulators: A Review Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-11-09 Zi-Lei Chen, Lin-Hui Yang, Ling-Ling Tong, Yue Wang, Meng-Zhen Liu, Dong-Sheng Guo
For the increasing demand for lipids and terpenoids in humans, biological fermentation has become an attractive choice due to the safety and sustainability. Thraustochytrids have been identified as promising producers of polyunsaturated fatty acids because of their high lipid content and simple fatty acid composition. In addition, thraustochytrids are also potential producers of terpenoids for their
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Changes in Fungal Community and Gene Expression of Specific Ligninolytic Enzymes in Response to Wood Decay Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-11-09 Min Lee, Endang Rahmat, Lynn Prewitt, Roggers Gang, Yeongjun Ban, Chang Ho Kang, Youngmin Kang
Microbial communities, particularly Basidiomycota fungi, play crucial roles in ecosystem functioning, including wood decay and the recycling of carbon and nutrients. However, the relationship between fungal diversity and wood decomposition remains poorly understood. This study investigated the effect of fungal community, gene expression, and activity of specific ligninolytic enzymes on wood decay in
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Chinese Hamster Ovary Cell Line Instability: Causes, Mitigation, and Prediction Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-11-09 Yun Joo, Hagyeong Kim, Jong Youn Baik
Cell line instability is a common problem in biopharmaceutical manufacturing using Chinese hamster ovary (CHO) cells. Cell line instability, which refers to unintended and unpredicted genetic, epigenetic, and phenotypic changes over time, can increase production costs and threaten sales approval by reducing product quantity and quality. While a stability test is conducted to screen cell lines with
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Design, Synthesis, In Silico Screening, and Antiproliferative Activity of Novel 1,2,3-Triazole Tethered Dibenzosuberane Conjugates Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-11-09 Ravi Kant, Keshav Kumar Saini, Ravindra Kumar Upadhyay, Yogender Singh, Y. Veera Manohara Reddy, Shishu Pal Singh, M. Abdul Kareem, K. R. Dasegowda, H. Prabhavati, Rakesh Kumar, Jong Pil Park, Lalita S. Kumar
To explore novel and potent compounds with anticancer activity, two series of 1H-1,2,3-triazole tethered dibenzosuberane conjugates (5a-i and 5j-n) were synthesized using a linear and convergent approach. The synthesized novel compounds were screened for their in vitro antiproliferative activity against HepG2 cell lines using the MTT assay to explore their binding interactions with the 5EQG protein
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Stability Enhancement of Target Enzymes via Tyrosinase-Mediated Site-Specific Polysaccharide Coating Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-11-09 Hyun Kim, Uk-Jae Lee, Gyu-Min Lim, Jin-Young Kim, Jeongchan Lee, Hanbit Song, Eun-jung Kim, Jungbae Kim, Nathaniel S. Hwang, Byung-Gee Kim
Enzymes are widely used in industrial and pharmaceutical applications, but their activities often decrease rapidly under harsh environmental conditions such as heat, organic solvents, and dehydration. In this study, a new method for enzyme coating with polysaccharide using a rapid tyrosinase-mediated crosslinking reaction was developed. When tyrosinase reacts with a monophenol-containing biopolymer
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Study on Anaerobic Digestion Characteristics of Hulless Barley Straw and Livestock Manure Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-11-09 Li Liu, Zhongping Du, Yi Li, Rui Han
Co-digestion performance of hulless barley straw mixed with pig, fowl, sheep, and cow manure induced by microbial changes were studied based on mesophilic batch anaerobic digestion (AD) experiments. The mixed AD of hulless barley straw and four kinds of manure yielded the synergistic promotion effect of materials. The highest methanogenic performance was achieved by AD of hulless barley straw mixed
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Alginate as a Soil Conditioner: Properties, Mechanisms, and Agricultural Applications Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-11-09 Hyo Jeong Shin, Hyun Uk Cho, Jong Moon Park
Soil plays a vital role in agriculture by providing essential nutrients, water, and support for crop growth. Therefore, the maintenance of healthy soil is imperative for the sustainability of agriculture and the assurance of food security. However, soil quality has been undermined by a range of factors, encompassing adverse environmental conditions, the impact of climate change, and inappropriate human
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Wickerhamomyces ciferrii Auxotroph and Expression Vector for Improved Production of Tetraacetyl Phytosphingosine Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-10-27 Seok Woo Yoo, Minsun Kim, Boo-Su Park, Jihee Yoon, Min-Kyu Oh
Tetraacetyl phytosphingosine (TAPS) is a precursor that has antibacterial and wound-healing properties and is used in the synthesis of ceramides. Wickerhamomyces ciferrii is a non-conventional yeast that has the ability to produce and secrete TAPS extracellularly. However, the genetic engineering tools available for this yeast species are limited. In this study, an auxotrophic strain Y94 of W. ciferrii
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Convolution Neural Network Approaches for Cancer Cell Image Classification Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-10-27 Chaeyoung Kim, Sungtae Shin, Sehoon Jeong
Recently, research incorporating the benefits of deep learning in the application of cancer cell classification and analysis has been actively conducted. In this paper, we investigated examples of binary-class classification and multi-class classification of cancer cell image data of commonly occurring types of cancer worldwide, such as cervical cancer, breast cancer, lung cancer, and colon cancer
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Multi-bioinspired Sprayable Nanotherapeutics for Tumor-Specific Focal Cancer Therapy Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-10-27 Yu Ri Jeon, Yun Kee Jo
Chemotherapy is commonly used to treat cancer. However, insufficient retention at the target site and systemic toxicity remains critical challenges. Chitosan has attracted considerable attention as a carrier backbone for delivering therapeutic cargos because of its biocompatibility and mucoadhesive property. However, pH and concentration-dependent solubility of chitosan has been restricted further
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Co-utilization of Maltose and Sodium Acetate via Engineered Corynebacterium glutamicum for Improved Itaconic Acid Production Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-10-17 Taghreed Elkasaby, Dao Duy Hanh, Hideo Kawaguchi, Masakazu Toyoshima, Akihiko Kondo, Chiaki Ogino
For growth and energy, Corynebacterium glutamicum has the ability to assimilate numerous carbon sources in the form of either single or combined substrates. During the growth of C. glutamicum on substrate mixtures, it has shown the ability to co-metabolize the majority of these carbon sources and displays monophasic growth, unlike other microorganisms such as Escherichia coli and Bacillus subtilis
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Elucidation of the Role of Gas Bubbles in the Fractional Precipitation of Paclitaxel Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-10-05 Da-Yeon Kang, Jin-Hyun Kim
In this study, the effect of gas flow rate and air stone pore size on the gas bubble-assisted fractional precipitation efficiency of paclitaxel was investigated. As the gas flow rate increased and the air stone pore size decreased, more numerous and smaller bubbles were generated and the bubble surface area increased. Additionally, at gas flow rates of 0.5 to 1.5 L/min, the precipitation yield and
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Hollow Spherical Curcumin Nanomicelles CUR@PLA@PF127: A New Trial on Breast Cancer Cells Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-10-05 Fatma S. El-Banna, Nemany A. N. Hanafy, Magdy E. Mahfouz, Maged El-Kemary
Curcumin (CUR) nanomicelles were developed by solvent-evaporation method using Poly (lactic acid) (PLA) for the first time with Pluronic F127 (PF127) to produce CUR@PLA@PF127 nanomicelles. The X-ray diffraction (XRD) spectra confirmed the encapsulation of CUR into the produced nanomicelles (CUR@PLA@PF127). Fourier transform infrared spectroscopy (FTIR) analysis showed the interactions of CUR with PLA
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Zero-valent Iron Enhances Acetate and Butyrate Production from Carbon Monoxide by Fonticella tunisiensis HN43 Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-09-15 Hyeon Sung Im, Da Seul Kong, Chae Ho Im, Changman Kim, Young Eun Song, Sang-Eun Oh, Jung Rae Kim
The use of carbon monoxide (CO) as a valuable feedstock for producing various platform chemicals through biorefinery processes has attracted considerable research interest. Acetate is an intermediate chemical synthesized from CO and CO2 through acetogenesis via the Wood–Ljungdahl pathway. Acetate can further serve as a substrate for chain elongation into a higher volatile fatty acid (VFA) when sufficient
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Transcriptional Characterization of the Effects of Macrophages on Inflammation-Induced Reconstructed Human Skin Equivalents Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-09-08 Yun Ji Cho, Bum Jun Jang, Yun Jung Yang, Hwa Sung Shin
Recently, artificial skin has been widely used to evaluate the biochemical safety and efficacy of certain drugs. Macrophages play a crucial role in the skin immune response by interacting with fibroblasts and keratinocytes. Transcriptional analysis is a cost-effective method to assess the immune response of macrophages in artificial skin. However, high variability in the mRNA levels of inflammatory
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Evaluation of Pretreatment and GABA Production Using Levilactobacillus brevis Fermentation of the Seaweed Saccharina japonica Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-08-30 Se Yeon Lee, Chae Hun Ra
The seaweed has a high content of easily degradable carbohydrates, making it a potential substrate for the production of γ-aminobutyric acid (GABA). In this study, response surface methodology pretreatment and enzymatic saccharification (Es) were conducted on a flask culture of Saccharina japonica seaweed. The optimal hydrolytic conditions were: 10.8% (w/v) slurry content, 0.7% H2SO4, and 121°C for
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Decreased Lincomycin B Content by Regulating Osmotic Pressure in Fermentation of Streptomyces lincolnensis Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-08-30 Shengkai Li, Liwen Zhang, Shuhong Gao
Lincomycin, a clinically important antibiotic against Gram-positive bacteria, is a sulfur-containing metabolite produced by Streptomyces lincolnensis. The main problem in the fermentation is the high content of lincomycin B, which is a by-product of insufficient methylation by S-adenosylmethionine (SAM) dependent methyltransferase. In this study, the content of lincomycin B was decreased from 4.04%
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Tuning the Assembly of Bispecific Antibodies by Playing on Differential Polypeptide Chain Molar Ratios Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-08-24 Célia De Temmerman, Pauline Bernard, Quentin Aeberli, Sarah Braito, Ghislaine Arib, Pierre-Alain Girod, Séverine Fagète
Bispecific antibodies (bsAb) represent the most promising class of therapeutics due to their multiple mode of action. Their development raised tremendous efforts with a special focus on the engineering of these complex molecules, especially when they adopt an asymmetrical format. Various strategies have been successful in improving heterodimerization through the introduction of point mutations to force
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High-Throughput Production of Microcapsules for Human Bone Marrow Derived Mesenchymal Stem Cell Biomanufacturing in a Vertical-Wheel Bioreactor Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-08-03 Matthew Teryek, Pankaj Jadhav, Raphaela Bento, Biju Parekkadan
Microencapsulation of human mesenchymal stromal cells (MSCs) via electrospraying has been well documented in tissue engineering and regenerative medicine. Herein, we report the use of microencapsulation, via electro-spraying, for MSC expansion using a commercially available hydrogel that is durable, optimized to MSC culture, and enzymatically degradable for cell recovery. Critical parameters of the
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Optimization of Vacuum Frying Process for Sweet Potato Chip Manufacturing Using Response Surface Methodology and Artificial Neural Network Model Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-07-31 Da-Song Kim, Joong Heon Lee, Hyun-Jae Shin
The purpose of this study was to optimize product yield and quality of the sweet potato chip manufacturing process in a pilot-scale industrial fryer using vacuum frying (VF) technology, response surface methodology (RSM), and artificial neural network (ANN) model. The variables, osmotic dehydration (OD) concentration, OD temperature, and VF temperature were designed to optimize the yield, oil content
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Near-infrared and Thermo-sensitive Liposomes Incorporating Thiolated-carboxymethyl Cellulose-capped Gold Nanoparticles and Poly(N-isopropylacrylamide) Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-07-25 Fanyu Zhao, Kwanjira Wangpimool, Jin-Chul Kim
Near-infrared (NIR) irradiation-responsive drug carriers are of great interest because the light is tissue-penetrating and the carriers administered into human body can be made to release their payloads at a specific site in an active manner using the external stimulus (i.e., NIR irradiation). In this study, thermo-responsive liposomes bearing gold nanoparticle (GNP) were developed as a NIR irradiation-responsive
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Drug-eluting Microneedles Embedded with Nanoparticles for Anti-inflammatory Purposes Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-07-25 Dongmin Kim, Seungho Baek, Anna Go, Heekyung Park, Rongen Ma, Donghyun Lee
Microneedle (MN) technology is used to deliver drugs transdermally in a minimally invasive manner with self-administered properties to enable controlled drug release. MNs can improve drug permeation efficiency into the skin by creating tiny holes through which the drugs can directly pass through the stratum corneum, which is the largest barrier layer of the skin. MNs can be used to deliver drugs transdermally
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Schisandrae Fructus Ameliorates Topical Particulate Matter 2.5-induced Keratoconjunctivitis Sicca That Are Accompanied by Retinal and Lipid Metabolism Disorders Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-07-25 Hyesook Lee, Hyun Hwangbo, Da Hye Kim, Min Yeong Kim, Seon Yeong Ji, Su Hyun Hong, Cheol Park, Chan-Young Kwon, Sun-Hee Leem, Gi-Young Kim, Yung Hyun Choi
Particulate matter 2.5 (PM2.5) is the biological toxic substances in the air pollutants, and causes to dry eye disease. To date, studies on the development of treatment for PM2.5-mediated dry eye disease are extremely limited. Schisandrae Fructus (SF), the fruit of Schisandra chinensis Turcz. (Baillon), has been used as traditionally herbal medicine in Northeastern Asia. SF has various pharmacological
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Enhancing the Thermostability of Cellulase from Clostridium thermocellum via Salt Bridge Interactions Biotechnol. Bioproc. E. (IF 3.2) Pub Date : 2023-07-06 Reyhane Zamani, Sayyed Shahryar Rahpeyma, Moein Aliakbari, Mousa Naderi, Mohsen Yazdanei, Saeed Aminzadeh, Jafar Khezri, Kamahldin Haghbeen, Ali Asghar Karkhane
Improving the thermal stability of enzymes is an essential factor in the industrial applications of enzymes. Many methods related to increased thermal stability were explained, and increasing salt bridges is one of the strategies for improving the thermal stability of enzymes. In this study, mutations T59E, I145R, N149R, V219D, and A262R are introduced into the native cellulase gene to produce the