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Expression analysis of miRNAs and their targets related to salt stress in Solanum lycopersicum H-2274 Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2021-01-12 Özgür Çakır; Burcu Arıkan; Burcu Karpuz; Neslihan Turgut-Kara
Abstract The microRNAs (miRNAs) are a group of small non-coding RNAs found in both plants and animals. Their functions and target genes have been identified in many plant species. It has been shown that miRNAs have crucial roles in stress responses of plants as much as other defence mechanisms. Throughout abiotic stress, miRNAs regulate their target genes and take part in signalling pathways. In this
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Molecular characterization and functional properties of avenin-like b gene TuALPb7As in Triticum urartu Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2021-01-07 Dong Cao; Fahui Ye; Caixia Zhao; Jicheng Shen; Ruijuan Liu; Bo Zhang; Yuhu Shen; Huaigang Zhang; Baolong Liu; Wenjie Chen
Abstract Avenin-like storage proteins affect the rheological properties and processing quality of common wheat. Triticum urartu, the A-genome donor of polyploid wheat, shows abundant variation in its glutenin subunits. In this study, the variability in avenin-like b genes was evaluated in 74 T. urartu accessions. High levels of polymorphism were demonstrated in the analyzed sequence, resulting in the
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Screening of enzyme-producing strains from traditional Guizhou condiment Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2021-01-06 Shaoqin Zhou; Haiying Zeng; Likang Qin; Yan Zhou; K. M. Faridul Hasan; Yingmei Wu
Abstract Broad bean sauce with chili (BBSC) is a traditional condiment in Guizhou province of China. In order to solve some problems especially lack of dominant strains during the fermentation of BBSC, enzyme-producing strains were screened and identified from BBSC. The counts of bacteria, yeasts and molds achieved a relative equilibrium at the later period of fermentation, with more than 2.6 × 104
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Ultrasound-assisted lipase-catalyzed synthesis of ethyl acetate: process optimization and kinetic study Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2021-01-05 Chengnan Zhang; Xin Liang; Abdullah Abdulaziz Abbod Abdo; Benariba Kaddour; Xiuting Li; Chao Teng; Chengyin Wan
Abstract Synthesis of ethyl acetate (EA) using lipase as a catalyst, as an environmentally-friendly process, has attracted considerable attention. However, the slow rate of enzymatic reaction and higher cost limit its applications. Ultrasound irradiation (UI) is known as an effective tool for accelerating the rate of enzymatic reaction under mild conditions. To attain maximum yield, it is crucial to
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Characterization, expression and function analysis of anthocyanidin reductase gene from Vitis vinifera L. cv. Cabernet Sauvignon under UV-C irradiation Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-12-30 Tiequan Niu; Changhen Li; Bo Yang; Pengfei Zhang; Weixin Fan; Pengfei Wen
Abstract Flavonoids are important polyphenols, and widely distributed in plants. Anthocyanidin reductase (ANR), a precursor enzyme in (-)- epicatechin synthesis, could play an important role in anthocyanins biosynthesis. In the present study, the ANR gene was cloned from Vitis vinifera L. cv. Cabernet Sauvignon. Compared with VvANR (GenBank Accession No. NM_001280956.1), they differed in only 4 bp
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Biotechnologies in the Czech Republic: current status, trends and challenges Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-12-30 Ivana Marova
Abstract This review presents an overview of the current status of the biotechnology sector in the Czech Republic (CR), its future trends and main challenges. CR is the country that laid down the principal laws of heredity, introduced the contact lens to the world and successfully developed the compounds on which current anti-AIDS drugs are based. The history of Czech biotechnology is strongly associated
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Association of paraoxonase (PON1) polymorphisms and activity with colorectal cancer predisposition Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-12-30 Tugrul Demirel; Ilhan Yaylim; Hayriye Arzu Ergen; Mustafa Kayihan Gunay; Yaman Tekant; Turgay Isbir
Abstract Paraoxonase 1 (PON1) is a well recognised member of human endogeneous free radical scavenging systems and its polymorphism and enzyme activity are attributed to various different cancer formations. We aimed to study the Paraoxonase 1 (PON1) polymorphism and enzyme activity in colorectal cancer patients. Peripheral blood samples for DNA extraction were collected from 54 colorectal cancer patients
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Protective effect of newly synthesized indole imines against ethanol-induced gastric ulcer in rats Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-12-30 Muhammad Shoaib Akhtar; Abdul Malik; Haroon Arshad; Sara Batool; Abdul Rauf Raza; Tahira Tabassum; Mian Anjum Murtaza; Muhammad Riaz; Misbah Noreen; Ghulam Rasool
Abstract Peptic ulcer is a common disorder of the gastrointestinal system affecting people of all ages worldwide. Ethanol is one of the most common causative agents for peptic ulcer. The aim of this study was to evaluate the protective effect of two newly synthesized indole imines (389 and 393) against ethanol-induced gastric ulcer in rats. Animals were orally administered different treatments: group
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High sensitivity of roots to salt stress as revealed by novel tip bioassay in wheat seedlings Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-12-30 Chiharu Nakamura; Shotaro Takenaka; Miyuki Nitta; Mikio Yamamoto; Tetsuya Kawazoe; Shunsuke Ono; Motoki Takenaka; Kazuma Inoue; Shotaro Takenaka; Shingo Kawai
Abstract Salinity is one of major environmental constraints that adversely affect plant growth and pose a serious threat to crop productivity. One basic concern is related to tissue sensitivity differences to salt stress. It is intriguing that much more research efforts have been directed to shoots/leaves than roots, although roots are the first tissues directly encountering salt stress and acting
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Effect of metformin and empagliflozin on adiponectin and leptin levels in rat model of hypo- and hyperthyroidism Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-12-28 Desimira Mironova; Teodora Hanjieva-Darlenska
Abstract Leptin and adiponectin secreted by the adipose tissue act in conjunction with thyroid hormones. The adiponectin/leptin ratio is a strong biomarker for cardio-metabolic health. The interrelations between the thyroid gland and adipokines are not yet clear. This study investigated whether anti-diabetic drugs, Metformin and Empagliflozin, lead to changes in leptin, adiponectin and their ratio
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Single nucleotide polymorphism mutation related genes in bladder cancer for the treatment of patients: a study based on the TCGA database Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-12-28 Tengyun Long; Xiaoni Li; Guofei Zhang; Chunming Qiu; Ouyang Huan; Canbiao Sun; Yong Yang
Abstract Bladder cancer (BLCA) is a common malignancy and has a poor prognosis. Single nucleotide polymorphisms (SNPs) in genes are closely associated with the tumorigenesis and tumor development and yet are not well illustrated in BLCA. In this study, we downloaded SNP‐related data and transcriptome profiling of BLCA from the Cancer Genome Atlas (TCGA) database and identified high frequency mutation
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Bioimage analysis of cell physiology of primary lens epithelial cells from diabetic and non-diabetic cataract patients Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-12-27 Vasil Haykin; Alexander Oscar; Violeta Dimitrova; Iva Petkova; Yani Zdravkov; Stanislava Kostova; Nevyana Veleva; Vanyo Mitev; Antonia Isaeva
Abstract Cataract is a multifactorial disease with increasing prevalence with age. Adult diabetics develop cataract earlier. Lens epithelial cells (LECs) exposed to oxidative stress (ROS), increased calcium deposit and membrane damage, undergo apoptosis, which results in lens opacification. Remaining LECs post-surgery leads to posterior capsular opacification (PCO). This study’s aim was to investigate
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Comparative analysis of RNA-Seq data reveals adventitious root development is mediated by ChIFNα in lotus japonicus Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-12-27 Shimei Yang; Siyu Ma; Riguang Qiu; Qiao Guang; Yun Lv; Yaping Dong; Jiahai Wu; Li Song
Abstract The formation of adventitious roots (ARs) plays an important role in grass growth and production. Chicken alpha interferon (ChIFNα) is an animal glycoprotein with broad activities of immune control, gene induction, growth and development regulation. ChIFNα gene was transformed to Lotus japonicus and its expression was found to promote AR development. The primordia formation occurred 3–5 days
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Analytical detection methods for diagnosis of COVID-19: developed methods and their performance Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-12-27 Ibrahim Hotan Alsohaimi
Abstract This mini review presents the standard methods for the analytical identification of virus species. These methods have been trusted and known as accredited methods. The development of new and improved analytical techniques is important to overcome the lack and the drawbacks of the well-known methods. Due to the uncertain and unprecedented time of pandemic, new approaches with high performance
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Evaluation of an enclosed air-lift photobioreactor (ALPBR) for biomass and lipid biosynthesis of microalgal cells grown under fluid-induced shear stress Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-12-17 Ning Ding; Chao Li; Tao Wang; Meijin Guo; Ali Mohsin; Siliang Zhang
Abstract An enclosed air-lift photobioreactor (ALPBR) is considered an efficient lab-scale bioreactor for microalgae cell growth and lipid biosynthesis. However, fluid-induced shear stress and mixing are two main factors that affect physiological metabolism in microalgal cell cultures. Herein, a 50-L ALPBR after being designed and manufactured was evaluated for microalgal suspension culture. Moreover
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Antibacterial mechanism of Aspergillus niger xj spore powder crude extract B10 against Agrobacterium tumefaciens T-37 Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-12-17 Kun Guo; Qinyu Zhang; Jinyi Zhao; Zhu Li; Jiang Ran; Yang Xiao; Su Zhang; Mianmian Hu
Abstract A crude extract (B10) of Aspergillus niger spores showed effective antibacterial activity against Agrobacterium tumefaciens T-37. The inhibition rate was 98.22%, and the half maximal inhibitory concentration was 0.035 ± 0.018 mg/mL. To study the primary antibacterial mechanism, this study used relative electric conductivity, release of proteins and nucleic acids, sodium dodecyl sulphate–polyacrylamide
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In vivo assessment of acute and subacute toxicity of ethyl acetate extract from aerial parts of Geum urbanum L Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-12-15 Lyudmila Dimitrova; Stanislav Philipov; Maya Margaritova Zaharieva; Jeni Miteva-Staleva; Milena Popova; Lilia Tserovska; Ekaterina Krumova; Galina Zhelezova; Vassya Bankova; Hristo Najdenski
Abstract Geum urbanum L. is a medicinal plant used from ancient time against gastro-intestinal disorders, bleeding and inflammation of mucous membranes, gums (gingivitis), etc. In our previous works, we demonstrated the antibacterial and antioxidant properties of ethyl acetate (EtOAc) extracts in vitro. The aim of this study was to evaluate the possibility of the EtOAc fraction from aerial parts to
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Development of an immunoassay for the detection of human IgG against hepatitis C virus proteins using magnetic beads and flow cytometry Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-12-15 Walter Luiz Lima Neves; Luis André Morais Mariuba; Késsia Caroline Souza Alves; Kerolaine Fonseca Coelho; Andrea Monteiro Tarragô; Allyson Guimarães Costa; Yury Oliveira Chaves; Flamir da Silva Victoria; Marilu Barbieri Victoria; Adriana Malheiro
Abstract Hepatitis C is a public health problem worldwide, affecting chronically about 71 million people, or 1% of the world population. The early detection of infection in clinical practice routine and blood donors is still important, being necessary for the development of new specific, sensitive, reliable tests that can improve the screening of HCV infection. Flow cytometry beads assay opens the
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Characterization of β-mannanase extracted from a novel Streptomyces species Alg-S25 immobilized on chitosan nanoparticles Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-12-15 Bidyut Ranjan Mohapatra
Abstract The potential applications of β-mannanase in bioenergy, biorefinery, detergent, fodder, food processing, oil and gas, pharmaceutical, pulp and paper and textile industries, and the high cost of commercially available enzyme have attracted considerable research efforts to identify potent microbial strains for cost-effective production of this enzyme. The advantage of microbial biocatalyst is
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DNA-free gene editing in plants: a brief overview Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-12-11 Tsveta Tsanova; Lidia Stefanova; Lora Topalova; Atanas Atanasov; Ivelin Pantchev
Abstract The conversion of bacterial CRISPR/Cas defense system into a simple and efficient tool for genome manipulations brought experimental biology into new dimensions. Suddenly, genome editing reached many groups most of which were interested in it but not able to employ the available time- and labor-consuming approaches of the pre-CRISPR era. In plant biology and biotechnology, CRISPR/Cas gene
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Bulked QTL-Seq identified a major QTL for the awnless trait in spring wheat cultivars in Qinghai, China Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-12-10 Dongxia Wang; Dong Cao; Yuan Zong; Yun Li; Jinmin Wang; Zongren Li; Baolong Liu
Abstract The awnless trait is the favorite trait for wheat breeding in Qinghai, China, but the major gene underlying the trait is unknown. This study aimed to analyze a recombinant inbred line (RIL) population containing 112 lines by crossing common wheat varieties GY448 (awnless) and GY115 (awned) using genotyping-by-sequencing analysis. A total of 56.62 Gb of clean sequence data were generated. A
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The virus that shook the world: questions and answers about SARS-CoV-2 and COVID-19 Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-12-08 Radostina Alexandrova; Pencho Beykov; Dobrin Vassilev; Marko Jukić; Črtomir Podlipnik
Abstract SARS-CoV-2 is an emerging human beta-coronavirus that caused the COVID-19 (Coronavirus Disease − 19) pandemic, the most significant health and social crisis in the last 100 years. SARS-CoV-2 is not only a respiratory virus; the symptoms of COVID-19 can include gastrointestinal, neurological, renal, cardiovascular and other complications. A large part of SARS-CoV-2 infected individuals are
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Physician's knowledge and experience with the off-label use of medicines Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-12-08 Maria Drenska; Emilia Naseva; Ilko Getov
Abstract The off-label use of medicines is neither prohibited nor explicitly allowed, according to the current European Union (EU) pharmaceutical legislation. This can cause confusion in physicians’ practice as well as lead to patients’ dissatisfaction. The purpose of this survey is to assess the knowledge and experience of Bulgarian physicians with the off-label use of medicines. The survey was performed
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Expression of nAChRα7 receptor in model rats with Parkinson’s disease dementia Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-12-07 Yan Pan; Jing Zhu; Lina Cong; Yang Bai; Yaxin Ma; Yue Yang
Abstract To investigate the expression and role of nicotinic acetylcholine α7 subunit (nAChRα7) in the hippocampus of rats with Parkinson's disease (PD) and Parkinson's disease dementia (PDD). PD model was established with 6-hydroxydopa. Then, methyllycaconitine was further used to prepare a PDD model. The behavioral indexes and malondialdehyde (MDA) and superoxide dismutase (SOD) levels were measured
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Functional profiling of bacterial communities in Lake Tuz using 16S rRNA gene sequences Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-10-31 Habeebat Adekilekun Oyewusi; Roswanira Abdul Wahab; Mohamed Faraj Edbeib; Mohd Azrul Naim Mohamad; Azzmer Azzar Abdul Hamid; Yilmaz Kaya; Fahrul Huyop
Abstract The 16S rRNA amplicon sequencing technique is a key aspect of studies of microbial communities but does not provide direct evidence of a community’s functional capabilities. This work aimed to assess the structure of the uncultured bacterial communities from two locations in Lake Tuz in Turkey to provide information on their roles in the lake ecosystem. The most abundant phyla in the lake
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Initial safety evaluation of Enterococcus faecium LBB.E81 Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-10-29 Zoltan Urshev; Tsvetelina Yungareva
Abstract Due to their antimicrobial activity Enterococcus faecium strains have a growing record of use as beneficial adjunct cultures in animal nutrition or components of probiotic preparations for humans. However, safety concerns accompany the application of enterococci, as clinical strains associated with infection have been isolated, especially antibiotic resistant cultures. With the increasing
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Biophysical characterization of the structural stability of Helix lucorum hemocyanin Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-11-03 Krassimira Idakieva; Svetla Todinova; Aleksandar Dolashki; Lyudmila Velkova; Yuliana Raynova; Pavlina Dolashka
Abstract The structural stability of the hеmocyanin purified from the hеmolymph of gаrden snаils Helix lucorum (HlH) was investigated by means of far-UV circular dichroism (CD), differential scanning calorimetry (DSC) and transmission electron microscopy (TEM). For the first time, TEM analyses showed the presence of tubular polymers in hemocyanins after three-day dialysis against a stabilizing buffer
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Knockout of the DAS gene increases S-adenosylmethionine production in Komagataella phaffii Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-11-04 Taiyu Liu; Baolin Liu; Hualan Zhou; Jianguo Zhang
Abstract S-adenosylmethionine (SAM) is an important compound in living organisms and has a number of different roles, such as polyamine synthesis. It can also be used for the treatment of diseases. Gene targeting with homologous recombination in Komagataella phaffii (K. phaffii) created DAS1 and DAS2 gene knockout strains, which use the methanol pathway to generate more ATP and increase SAM production
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Respiratory viruses crossing the species barrier and emergence of new human coronavirus infectious disease Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-11-10 Nikolay Kalvatchev; Ivo Sirakov
Abstract In the context of the current pandemic caused by the new coronavirus SARS-CoV-2, it is essential to note that a new human respiratory virus has been identified almost every year in the last two decades. This brief summary outlines the airborne respiratory viruses discovered among the human population in recent years and their pandemic potential. The focus falls on the coronaviruses: cell interaction
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Comparative transcriptome analysis of lingonberry (Vaccinium vitis-idaea) provides insights into genes associated with flavonoids metabolism during fruit development Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-11-19 Youwen Tian; Zhili Ma; Haohao Ma; Yu Gu; Yadong Li; Haiyue Sun
Abstract Because the genomic information of lingonberry (Vaccinium vitis-idaea L.) is not available, the mechanism underlying the flavonoid metabolism in the fruits of this species is still unknown. The objective of this study was to obtain a comprehensive transcriptome assembly during lingonberry fruit development and characterize the key genes associated with the flavonoid metabolism in lingonberry
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Diversification of animal gut microbes and NRPS gene clusters in some carnivores, herbivores and omnivores Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-11-09 Xiurong Guo; Hui Lei; Kailian Zhang; Famin Ke; Can Song
Abstract The animal gut has a remarkable abundance of microbes. These microbes have strong potential for biosynthesis of promising drug candidates, and these small bioactive molecules often medicate important host–microbe and microbe–microbe interactions. To understand the evolution of the animal gut microbes and their biosynthetic gene clusters, we characterized 24 faecal samples from different herbivores
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Ophthalmic complaints in face-mask wearing: prevalence, treatment, and prevention with a potential protective effect against SARS-CoV-2 Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-10-31 Evelina Marinova; Dimitar Dabov; Yani Zdravkov
ABSTRACT: The global pandemic of severe acute respiratory syndrome, COVID-19 affects the world’s economics, social and health systems and has a great impact on every person’s life. In an attempt to control the spread of the disease, regular usage of face masks became an ordinary routine. A large proportion of the population is reporting ocular symptoms through the period of mask-wearing that are still
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Enhancement of l-amino acid oxidase production by Bacillus subtilis HLZ-68 with oxygen-vector and asymmetric degradation of dl-arginine to d-arginine Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-10-22 Peng Xu; Changpei Pan; Gongcheng Cui; ChunYan Wei; Lijuan Wang; Yanting Li; Xiangping Li; Shihai Huang
Abstract Bacillus subtilis HLZ-68 can produce l-amino acid oxidase (l-AAO), and dl-arginine can be degraded asymmetrically by suspending the wet bacterial biomass in the degradation liquid. By adding oxygen-vectors to the fermentation medium, the collected amount of wet bacterial biomass can be increased. Taking n-dodecane, n-hexadecane, oleic acid, paraffin and n-hexane as oxygen-vectors, the optimal
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Molecular characterization and bioactive potential of newly identified strains of the extremophilic black yeast Hortaea werneckii isolated from Red Sea mangrove Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-10-22 Mohamed Salah El-Din Hodhod; Abdel-Rhman Zakaria Gaafar; Aref Alshameri; Ahmed Ali Qahtan; Abdulrahman Noor; Mohamed Abdel-Wahab
Abstract The adaptations that halophilic microorganisms have developed due to their extreme habitat, promote the production of active natural compounds with the potential to control microorganisms causing infections associated with health care. The prime purpose of this study was to isolate and identify extremophilic black yeast Hortaea werneckii from intertidal decayed leaves of Avicennia marina located
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Paroxetine can improve primary visual cortex activity in a high-risk mouse model of schizophrenia Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-10-22 Xinying Chen; Ziyao Cai; Feng Ji; Xiaodong Lin; Deguo Jiang; Chongguang Lin; Xiaoyan Ma; Yong Xu; Wenqiang Wang; Lidan Zheng; Ce Chen; Chuanjun Zhuo
Abstract Visual cortex functional deficits can be observed in schizophrenia patients and in individuals at high risk of schizophrenia. However, to date, few studies have investigated methods to improve these functional deficits. This study aimed to investigate the pathological change in the primary visual cortex of a prenatal MK-801-induced high-risk mouse model of schizophrenia (HRMMS) and to test
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Genome-wide identification and expression analysis of the citrus calcium-dependent protein kinase (CDPK) genes in response to arbuscular mycorrhizal fungi colonization and drought Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-10-25 Bo Shu; Dengwei Jue; Fei Zhang; Dejian Zhang; Chunyan Liu; Qiangsheng Wu; Chun Luo
Abstract The calcium-dependent protein kinases (CDPKs, also termed CPKs) are a large, diverse protein family that play significant roles in biotic and abiotic stress responses in plants. However, CDPKs in citrus are relatively unknown, as are their responses to drought stress (abiotic stress) and arbuscular mycorrhizal fungi (AMF) colonization. In this study, the citrus CDPKs were characterized by
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Post thaw treatment of frozen buffalo semen with antioxidants vitamin C and 2-mercaptoethanol Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-10-23 Maria Ivanova; Desislava Abadjieva; Desislava Gradinarska; Omaima Kandil; Ahmed Abdoon; Paulina Taushanova; Boyko Georgiev
Abstract The aim of this study was to evaluate the effect of ascorbic acid (Vitamin C) and 2-mercaptoethanol (ME) added to buffalo semen samples after thawing for 1 h and 2 h incubation. Ejaculates (n = 20) from eight Bulgarian Murrah buffaloes were frozen in pellets and straws at a final sperm concentration of 80 × 106 mL. We investigated the effect of supplementations on the motility, morphology
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A novel sequence-based prediction method for ATP-binding sites using fusion of SMOTE algorithm and random forests classifier Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-10-28 Jiazhi Song; Guixia Liu; Chuyi Song; Jingqing Jiang
Abstract Correctly identifying the protein-ATP binding site is valuable for both protein function annotation and new drug discovery. However, the number of non-ATP-binding residues is much more than the number of ATP-binding residues, which makes the prediction a classical imbalanced learning problem. Previous studies often apply the under-sampling technique to construct a relatively balanced dataset
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Potential biological mechanisms underlying the endangered status of Glehnia littoralis revealed by nrDNA ITS and RAPD analyses Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-10-19 Weiwei Li; Bin Li; Ping Zhang; Dechang Hu; Ailan Wang
Abstract Certain biological mechanisms of Glehnia littoralis, an endangered medicinal plant in China, may be underlying its endangered status and this has attracted extensive attention from researchers. In this study, nuclear ribosomal DNA internal transcribed spacer (nrDNA ITS) sequences and random amplified polymorphic DNA (RAPD) were investigated to determine the genetic diversity of G. littoralis
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Genetic and flower volatile diversity in two natural populations of Hyssopus officinalis L. in Bulgaria Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-10-19 Tzvetelina Zagorcheva; Krasimir Rusanov; Mila Rusanova; Ina Aneva; Ira Stancheva; Ivan Atanassov
Abstract The genetic and flower volatile diversity of 59 plants from two natural populations of Hyssopus officinalis L. situated 73 km away from each other in typical growing regions for this species in Bulgaria were analysed. Analysis with 10 sequence related amplified polymorphism (SRAP) primer combinations scored a total of 1045 peaks of which 88.7% were polymorphic. Principal Coordinate Analysis
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Purification and characterization of a novel bacteriocin produced by Enterococcus faecalis CG-9 from human saliva Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-10-12 Gongcheng Cui; Changpei Pan; Peng Xu; Yanting Li; Lijuan Wang; Binting Gong; Xiangping Li; Shihai Huang
Abstract Strain CG-9, isolated from human saliva and identified as Enterococcus faecalis, produced a component that inhibits the growth of Gram-positive and Gram-negative bacteria. The active peptide from the cell-free supernatant of Enterococcus faecalis CG-9 was purified in three steps: (1) precipitation with 70% saturated ammonium sulfate: (2) cation-exchange chromatography, (3) gel filter chromatography
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Gene expression profiles and related immune-inflammatory factors in the cerebral arteries in mouse models of subarachnoid haemorrhage Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-10-10 Yi Wang; Zhenfeng Han; Bo Wang; Yuanbo Luo; Shuai Zhou; Zengguang Wang; Ye Tian; Jianning Zhang
Abstract The study aimed to identify key genes involved in cerebral vasospasm (CVS) after subarachnoid haemorrhage (SAH). GSE46696 of basilar arteries of SAH mice and normal controls were downloaded from the Gene Expression Omnibus (GEO). Integrated microarray analysis was performed to identify differentially expressed genes (DEGs). GO and KEGG pathway enrichment analyses of DEGs were performed with
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Identification of candidate genes associated with photosynthesis in eggplant under elevated CO2 Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-10-06 Xiaoyong Xu; Peiqi Wu; Hongxia Song; Jing Zhang; Shaowen Zheng; Guoming Xing; Leiping Hou; Meilan Li
Abstract Elevated CO2 can stimulate plant growth in greenhouses, but the molecular mechanism behind this response is unclear, especially in eggplants. In this study, we evaluated the physiological and photosynthetic response of the eggplant variety ‘Shenyuan 3’ under elevated CO2, and the candidate genes related to this response were identified by transcriptomic analysis. The results showed that elevated
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Characterization of mTERF family in allotetraploid peanut and their expression levels in response to dehydration stress Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-10-05 Limei Li; Bo Hu; Xiaoyun Li; Ling Li
Abstract Polyploidization events are prevalent in plants and act as an important evolutionary force for speciation, adaptation and diversification. During this process, the genome undergoes reorganization and large changes. However, the evolutionary characteristics of specific gene families are still not well investigated. Cultivated peanut is an allotetraploid that evolved from a diploid. In this
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Contribution of photosynthetic- and yield-related traits towards grain yield in wheat at the individual quantitative trait locus level Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-10-06 Ma Yu; Hua Chen; Shuang-Lin Mao; Kai-Mi Dong; Da-Bin Hou; Guo-Yue Chen
Abstract Grain yield improvement in wheat (Triticum aestivum L) requires understanding of the genetic control of grain yield components and other related traits. In this study, a total of 82 and 314 quantitative trait loci (QTLs) were associated with grain yield and grain yield components after harvest and photosynthetic traits at the seedling, heading and grain filling stages from a recombinant inbred
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DIA (Data Independent Acquisition) proteomic based study on maize filling-kernel stage drought stress-responsive proteins and metabolic pathways Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-10-06 Yatong Yang; Anyi Dong; Tinashe Zenda; Songtao Liu; Xinyue Liu; Yafei Wang; Jiao Li; Huijun Duan
Abstract Drought is one of the most severe environmental stressors hampering maize (Zea mays L.) production worldwide. Therefore, revealing the molecular mechanisms underpinning maize drought tolerance is critical for guiding drought resistance breeding and ensuring global food security. Herein, we performed a data-independent acquisition (DIA) proteomics-based comparative analysis of the proteome
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Extraction, identification and mechanism of action of antibacterial substances from Galla chinensis against Vibrio harveyi Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-10-06 Lei Guo; Xintong Wang; Jia Feng; Xiaowen Xu; Xiangrong Li; Wenbin Wang; Yingying Sun; Fuquan Xu
Abstract The aim of this study was to investigate the extraction of antibacterial substances from Galla chinensis (ASGC) that act against the aquatic pathogen Vibrio harveyi, to identify the chemical composition and explore the mechanism of action. The optimal extraction process was as follows: anhydrous methanol, liquid/material ratio (mL/g) 26:1, extraction temperature 98 °C and extraction time 157 min
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EST-SSR markers development and application in an important medicinal plant, Schisandra chinensis (Schisandraceae) Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-09-29 Guangli Shi; Shuang Li; Zhenxing Wang; Dan Sun; Susu Zhang; Jianhui Guo; Jun Ai
Abstract Schisandra chinensis, classed as a climbing woody vine, is representative of the Schisandraceae family regarded as a significant plant in Chinese herbal medicine. However, the application of molecular breeding is constrained by the fact that the number of genetic markers is small for this species. In this study, transcriptome sequencing of S. chinensis was performed using the Illumina HiSeq
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Regulation and marketing of cannabidiol-containing products in European countries. Pharmacists’ knowledge in Bulgaria Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-09-29 Bogdan Kirilov; Margarita Zhelyazkova; Elina Petkova-Gueorguieva; Georgi Momekov
Abstract To assess the current regulatory frameworks of cannabidiol-containing products/preparations on the European market, we analyzed data gathered from 19 European countries using the functionalities of the computer software EPITT (European Pharmacovigilance Issues Tracking Tool). In addition, we investigated pharmacists’ knowledge, awareness and attitudes toward cannabidiol (CBD) in Bulgaria.
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Endoscopic surgical resection of tuberculum sellae meningiomas based on decision-making algorithms proposed in the literature Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-09-25 Danny Kolev; Asen Hadzhiyanev; Marin Marinov; Asen Bussarsky; Deyan Popov; Vasil Karakostov
Recent publications and studies propose using decision-making algorithms in choosing the optimal approach for each individual case of tuberculum sellae meningioma (TSM). Minimally invasive endoscopic approaches offer the possibility of early devascularization, reduced brain and nerve retraction and speedy patient recovery. We used the decision-making algorithm proposed in the literature to choose between
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AMMI analysis of genotype × environment interaction on grain yield of sesame (Sesamum indicum L.) genotypes in Iran Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-09-24 Hossein Movahedi; Khodadad Mostafavi; Majid Shams; Ahmad Reza Golparvar
In this study, we investigated the effect of genotype × environment interaction (GEI) on the grain yield of 15 different sesame genotypes on four test areas (Arak, Birjand, Karaj and Shiraz) in two years (2017–2018). We observed significant differences in all sources of combined variance analysis. The interaction items in AMMI (additive main effects and multiplicative interactions) analysis of variance
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Full-length tran sequencing provides insight into lignan and lignin metabolism relation Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-09-24 Dongwei Xie; Zhigang Dai; Jian Sun; Jianguang Su
Flax (Linum usitatissimum L.) is an important economic crop worldwide. The lignin content of flax stems directly determines the quality of flax fiber, and excessively high lignin content in the fiber leads to pollutant discharge by the textile industry. Flax seeds also contain lignans, a group of compounds that are structurally similar to lignin and highly beneficial for human health. The biosynthesis
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Genome-wide identification and expression analysis of the carotenoid metabolic pathway genes in pepper (Capsicum annuum L.) Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-09-24 Qinwei Guo; Huiqin Liu; Xinhui Zhang; Ting Zhang; Chaosen Li; Xiaomin Xiang; Wenhao Cui; Pingping Fang; Hongjian Wan; Chunxin Cao; Dongfeng Zhao
Carotenoids belong to a major family of isoprenoids which play vital roles in plant growth and development. Pepper (Capsicum annuum L.) is not only a vegetable and condiment grown worldwide, but also can be used as a model organism in fruit color research. In this study, 61 genes known to be involved in mevalonate (MVA), plastidial 2-C-methyl-d-erythritol-4-phosphate (MEP) and carotenoid metabolism
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Virulence of Purpureocillium lilacinum strain ZJPL08 and efficacy of a wettable powder formulation against the Asian citrus psyllid (Diaphorina citri) Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-09-22 Danchao Du; Lianming Lu; Xiurong Hu; Zhanxu Pu; Zhendong Huang; Guoqing Chen; Shunmin Liu; Jia Lyu
The Asian citrus psyllid, Diaphorina citri Kuwayama, is one of the most destructive pests of citrus and the primary vector of Candidatus Liberibacter spp., which cause citrus Huanglongbing (HLB). Microbial insecticides based on entomopathogenic fungi are safer alternatives to chemical pesticides. They can mitigate the spread of HLB by suppressing D. citri populations in citrus groves. Purpureocillium
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Understanding the chemical foundation and genetic mechanism of the black grain trait in quinoa by integrating metabolome and transcriptome analyses Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-09-17 Dongxia Wang; Dong Cao; Youhua Yao; Jinmin Wang; Zongren Li; Baolong Liu
Quinoa (Chenopodium quinoa Willd.) cultivars that produce black grains are thought to be more nutritious and contain powerful antioxidant properties, but little is known about the chemical foundation and the genetic mechanism behind this trait. In this research, widely-targeted metabolome and transcriptome analyses were employed to unveil the chemical and genetic differences between black and white
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Polymorphisms of C-reactive protein and growth arrest-specific gene 6 modulate ischemic stroke susceptibility through gene–gene interactions in Chinese Han population Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-09-16 Xiaofeng Li; Yongjun Gao; Xiaoli Zhao; Zhongyun Chen; Xinyi Li; Xu Yang
This study explored whether inflammatory gene including C-reactive protein (CRP) and growth arrest-specific gene 6 (GAS6) are related to the etiology of ischemic stroke (IS), and to verify their potential interaction upon susceptibility to IS in Chinese population. A total of 236 patients with IS and 291 non-IS subjects were enrolled. Five single nucleotide polymorphisms (SNPs) in two candidate genes
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Assessment of the genetic diversity and population structure of Sophora tonkinensis in South China by AFLP markers Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-09-17 Zhu Qiao; Dong Xiao; Chanthaphoone Keovongkod; Kun-Hua Wei; Long-Fei He
Sophora tonkinensis is an important source of herbal medicine for treatment of swelling and pain in the throat. Its chemical composition has been extensively studied; however, there are still gaps in the research on genetic diversity of S. tonkinensis which can provide insights for better utilization and conservation of genetic resources. In this study, 23 populations of diverse locations from southern
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Optimization of “Zaoheibao” wine fermentation process and analysis of aroma substances Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-09-17 Guofeng Duan; Yuanyuan Liu; Hongliu Lv; Fei Wu; Rufu Wang
The “Zaoheibao” fruit with rich rose-scented grapes was used as the main material to optimize the fermentation process of “Zaoheibao” wine. Its composition in volatile aroma compounds was determined by headspace solid phase microextraction (HS-SPME) and gas chromatography–mass spectrometry (GC–MS) analysis, indicating that “Zaoheibao” wine has strong ester, fruity and floral characteristics. The addition
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Morphological description, genetic diversity and population structure of safflower (Carthamus tinctorius L.) mini core collection using SRAP and SSR markers Biotechnol. Biotechnol. Equip. (IF 1.186) Pub Date : 2020-09-14 Seyed Mohammad Reza Hassani; Reza Talebi; Sayyed Saeid Pourdad; Amir Mohammad Naji; Farzad Fayaz
This study was conducted to investigate the genetic diversity and population structure of globally diverse mini core collection of safflower (Carthamus tinctorius L.) using agro-morphological, sequence-related amplified polymorphism (SRAP) and simple-sequence repeats (SSR) molecular markers. Field experiment results showed significant differences between genotypes for agro-morphological traits such