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Investigation of HEG1, VISTA (VSIR) and PD-L1 expression levels in malignant mesothelioma by immunohistochemical methods and their relationship with histological subtypes Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2024-03-13 Medine Dicleli, Ayşe Nur Keleş, Ulaş Alabalik
Malignant Mesothelioma is a primary malignant tumor of the mesothelium lining the pleura, pericardium, peritoneum and tunica vaginalis of the testis with a poor prognosis. The epithelioid subtype i...
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Multispecies colonisation and surface erosion on A106 GB industry-finished steel used in heat exchangers Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2024-03-11 Alicia Prithiraj, Shepherd Tichapondwa, Jackie Nel, Evans Chirwa
Multispecies bacterial attachment to carbon steel surfaces is not fully understood; for example, as to why the attachment of certain bacteria influences corrosion. In this study, finished steel, A ...
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Salicornia europaea L. and Suaeda maritima (L.) Dumort: bioactive compounds and future perspectives Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2024-03-11 Velina Dzhoglova, Kalin Ivanov, Diana Karcheva-Bahchevanska, Nina Koleva, Maria Bozhkova, Niko Benbassat, Stanislava Ivanova
Halophyte plants have a phytochemical composition that results from the harsh conditions characteristic of their specific habitats. These plants have been used since ancient times because of the va...
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Bacterial diversity and functional characteristics of natural rainwater lakes in Saihanba artificial forest farm as revealed by 16S rRNA sequencing Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2024-03-11 Xinrui Gou, Yanli Liu, Kun Li, Chunyan Tian, Jiahao Cui, Shiqi Xu
The Saihanba Mechanical Forest is an artificial national forest park with a forest-steppe landscape. The flora and fauna have been extensively studied, but a comprehensive understanding of the bact...
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Genetic diversity and phylogeography of Japanese brome (Bromus japonicus Thunb. ex Murr.) populations in China Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2024-03-11 Qi Li, Jinping Yu, Long Du, Penghua Bai, Wei Zhang, Yixue Liu
Japanese brome (Bromus japonicus Thunb. ex Murr.) is a pervasive annual weed with wide distribution in winter wheat fields across the North China Plain. In this study, we researched the genetic div...
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A galantamine–curcumin hybrid lacks the depressant side effect of acetylcholinesterase inhibitors Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2024-01-29 Ivanka Kostadinova, Mariyana Atanasova, Georgi Stavrakov, Irena Philipova, Irini Doytchinova
Acetylcholinesterase inhibitors (AChEIs) are drugs that enhance cholinergic neuro-transmission and are used for the treatment of Alzheimer’s disease and myasthenia gravis. Common AChEIs include riv...
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Multi-objective parameter estimation on cultivation of yeast Kluyveromyces marxianus var. lactis MC5 Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2024-01-08 Mitko Petrov
In this study, the most appropriate form of the specific growth rate of the yeast Kluyweromyces marxianus var lactis MC5 was determined by a multi-criteria decision-making method (PROMETHEE II). Th...
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Utilization of plant secondary metabolites for plant protection Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-12-27 Aneta Lyubenova, Liliya Georgieva, Veselka Antonova
In the past two decades, the need for a new concept in agriculture has emerged. The new “Greener revolution” should rely on the implementation of sustainable practices in crop production and the ac...
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Selected rhizobacteria strains as potential growth promoters and biocontrol agents against chocolate spot disease in faba bean grown in pots Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-12-26 Gebeyehu Yibeltie Mengstie, Zewdu Teshome Awlachew, Atsede Muleta Degefa
The faba bean is a grain legume used for food and feed. It suffers from several yield limiting diseases. Botrytis fabae Sard is a fungal pathogen which causes chocolate spot disease in faba bean al...
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Restoring mitophagy in prostate cancer cells: the role of miR-141 rescue in counteracting MAPK1/ERK2-dependent autophagy suppression Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-12-18 Radostina Tsvetankova, Ilka Tsvetkova, Soren Hayrabedyan, Krassimira Todorova
Prostate cancer (PCa) stands as one of the most prevalent malignancies in men, ranking as the fifth leading cancer-associated mortality. Castration-resistance metastatic phenotype acquisition is su...
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Production and biochemical characterization of keratinase enzyme from Bacillus subtilis ES5 and its potential application in leather dehairing process: a clean leather tanning process Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-12-11 Getachew Alamnie, Amare Gessesse, Misganaw Bitew, Nebiyu Dawud, Berhanu Andualem, Abayeneh Girma
The traditional leather industry releases different chemical wastes into the environment, which calls for the use of alternative, nonpolluting processes. The use of biological catalysts in industri...
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Identification of QTL controlling the ratio of linalool to linalyl acetate in the flowers of Lavandula angustifolia Mill var. Hemus Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-12-09 Krasimir Rusanov, Pavlina Vassileva, Mila Rusanova, Ivan Atanassov
The content of linalyl acetate and the ratio of linalool to linalyl acetate (L/LA ratio) are one of the important parameters that determine the quality of lavender oil. The characterization of a se...
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Placenta accreta spectrum: ultrasound diagnosis and clinical correlation Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-11-30 Mariana Tsankova, Mila Kirkova, Nikolay Geshev
The abnormal invasive placenta is a unique pathological condition, and it occurs only in humans. The definitions, classifications, and ultrasound markers for pathological placental attachment are b...
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Optimization of intracellular L-Asparaginase production by Streptomyces paulus CA01 isolated from wheat bran using the response surface methodology Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-11-29 Achour Chergui, Lamia Trabelsi, Mounir M. Salem-Bekhit, Mohamed H. Mahmoud, Ehab I. Taha, Mohamed A. Ibrahim, Alessandro Erto, Ali Imessaoudene, Mouloud Kecha, Yacine Benguerba, Karim Houali
This study aimed to enhance the production of the intracellular L-asparaginase bacterial enzyme, which is known to impede the growth of cancer cells, by optimizing the culture conditions. In the pr...
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What is the lowest lethal dose of colchicine? Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-11-27 Vanyo Mitev
Colchicine is known for its narrow therapeutic index, unclear boundaries between non-toxic, toxic and lethal doses and limited use due to its toxicity. In the scientific literature the opinion has ...
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Potential of circulating tumour DNA as a biomarker in liquid biopsy Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-11-27 Dimitar Dimitrov, Hristo Ivanov, Nelly Miteva-Marcheva, Vili Stoyanova
Modern oncology has been influenced and transformed by precision and personalized medicine, especially with the introduction of personalized therapy. The ever-increasing knowledge of the genome con...
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Analysis of blood microbiome dysbiosis in pulmonary sarcoidosis by decision tree model Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-11-22 Yordan Hodzhev
Pulmonary sarcoidosis is a complex inflammatory disease characterized by granulomas in the lung tissue, leading to breathing difficulties and chest pain. Its etiology remains not fully understood, ...
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Analyzing real-world data to understand COVID-19 vaccination effects on hospitalization rates in patients with solid malignancies Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-11-19 George Dimitrov, Radka Argirova, Trifon Valkov
The impact of COVID-19 vaccination on hospitalizations in individuals with solid malignancies remains uncertain. This nationwide retrospective study aims to clarify this issue. We analyzed data fro...
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High cross-pollination rate of Greek oregano (O. vulgare ssp. hirtum) with Common oregano (O. vulgare ssp. vulgare) under open field conditions as revealed by microsatellite marker analysis Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-11-07 Marina Evgenieva Alekseeva, Mila Gradeva Rusanova, Liliya Nikolova Georgieva, Krasimir Emilov Rusanov, Ivan Iliev Atanassov
We studied the mode of pollination in Greek oregano (Origanum vulgare ssp. hirtum) under both controlled and open pollination conditions. When grown indoors without the presence of insects, Greek o...
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Bioinformatics analysis and prokaryotic expression of a cystatin analogue from Spirometra erinaceieuropaei Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-11-07 Lin Huang, Ling Mai, Gang Lv, Xinjun Chen
Cystatin plays a crucial role in immune evasion by parasites. It regulates diverse immune response processes, such as antigen presentation, cytokine and NO production, and phagocytosis. In recent y...
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Traditional microbial control methods used in sake brewing effectively suppress predominant bacteria emerging during production of rice koji Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-10-24 Kota Naganuma, Youji Nakagawa, Susumu Kokubo, Takuya Hashimoto, Kayo Higuchi, Naoko Ariizumi, Masayuki Hayakawa, Hideki Yamamura
Rice koji is a raw material used in the production of sake; however, details regarding the microbial flora and their dynamics in rice koji during the production process are poorly understood. Clari...
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Adequate effectiveness of ribociclib plus letrozole or fulvestrant in patients with advanced or metastatic hormone receptor-positive, human epidermal growth factor receptor 2-negative breast cancer treated in routine Bulgarian clinical practice Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-10-19 Manoela Manova, Jeliazko Arabadjiev, Radoslav Mangaldzhiev, Assen Dudov, Daniel Penchev, Margit Hemetsberger, Arkadi Sharkov, Boryana Zidarova, Dimitrina Apostolova, Maria Vasileva, Silvia Terezova, Mila Vlaskovska, Alexandra Savova
Randomized controlled trials (RCTs) are typically conducted in highly selected patient populations to ideally demonstrate unconfounded clinical efficacy of a drug. In the real world, there may be c...
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Cost-effectiveness of velmanase alfa vs. bone marrow transplantation or no causal therapy in patients with mild to moderate alpha-mannosidosis Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-10-23 Ana Antanasković, Ivana Stević, Refet Gojak, Dragana Lakić, Slobodan Janković
Alpha-mannosidosis is an inherited rare disorder of mannose-containing oligosaccharides metabolism that is currently treated by enzyme replacement therapy (ERT), bone marrow transplantation (BMT), ...
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TLR4 signalling protects Sertoli cells from cell stress via reprogramming inflammasome and autophagy pathways in MAPK1/ERK2 dependent way Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-10-18 Elina Avramska, Ilka Tsvetkova, Krassimira Todorova, Soren Hayrabedyan
Sertoli cells constitutively maintain the spermatogonial stem cell niche and blood-testis barrier via impenetrable tight junctions under hormonal, growth factor and cytokine control. Immature Serto...
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Immunity to mumps virus in children population in Bulgaria Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-10-18 Stefka Krumova, Radostina Stefanova, Savina Stoitsova, Petia Genova-Kalou, Kremena Parmakova
Mumps is an acute, contagious, viral vaccine-preventable disease caused by mumps virus (MuV). The measles, mumps, rubella (MMR) vaccine that is used in many countries is considered highly effective...
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Microsatellite unstable colorectal cancers are associated with increased CD1a- and CD83-positive dendritic cell infiltration Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-10-12 Maya Gulubova, Elina Aleksandrova, Yovcho Yovchev, Dimitar Chonov, Petio Chilingirov, Tatyana Vlaykova
Microsatellite instability (MSI) is characterized by a highly immunogenic tumor phenotype and abundant lymphocytic infiltrates. The aim of this study was to investigate the association between four...
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Bigel formulations of St. John’s wort extract in wound healing: toxicological aspects Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-10-06 Yoana Sotirova, Deyana Vankova, Oskan Tasinov, Stefka Stoeva, Minka Hristova, Ivelin Iliev, Svetlana Georgieva, Yoana Kiselova-Kaneva, Velichka Andonova
This study aimed to investigate the toxicological profile of hyperforin (HP) in silico and to assess it in vivo after topical application of an HP-rich St. John’s wort (SJW) extract. The former ana...
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Overexpression of GhSWEET42, a SWEET-like gene from cotton, enhances the oil content and seed size Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-10-08 Deying Wang, Mengxue Du, Jingyu Li, Shuwen Yin, Yongwang Sun, Shangjing Guo
SWEET (‘sugars will eventually be exported transporters’) family genes reportedly play a critical role in sugar translocation and oil biosynthesis in various plant species. However, their functions...
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Enhancement of taxane production in cell suspension culture of Corylus avellana L. Kalınkara by elicitation and precursor feeding Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-10-02 Ilayda Goktepe-Atilgan, Ahmet Dogan, Sule Ari
Paclitaxel (trademark Taxol®) is the most effective chemotherapeutic agent against a wide range of cancers. Hazelnut (Corylus avellana L.) cell culture is a promising and inexpensive strategy for p...
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Flaxseed protein isolate-alginate microbeads as encapsulating agents for enhanced survival of Enterococcus faecalis HZNU S1 during storage and in vitro gastrointestinal conditions Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-10-02 Zhen-Xing Tang, Zheng Xu, Qing-Nan Ma, Xue-Yan Chen, Hang-Qi Chen, Yu-Jie Cao, Tao-Yang Zhang, Zhen-Bo Yao, Lu-E Shi
This study aimed to study the entrapment of Enterococcus faecalis HZNU S1 using a mixture of flaxseed protein isolate (FPI) and sodium alginate (SA) as an encapsulation agent. The characteristics o...
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A study of anti-SARS-CoV-2 antibodies among healthcare personnel and the general population Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-09-14 Iva Trifonova, Kim Ngoc, Teodora Gladnishka, Vladislava Ivanova, Metodi Kunchev, Ventsislav Mutafchiyski, Iva Christova
Abstract Bulgaria, like many other countries, has experienced the far-reaching consequences of the Coronavirus disease 2019 (COVID-19) pandemic. Seroprevalence studies serve as a crucial method for assessing the exposure and immunity levels within a population. In this serosurvey, which is the first of this kind conducted in Bulgaria, we enrolled 104 healthcare workers (HCWs) from one front-line Bulgarian
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Ellagic acid for oral mucositis: investigation of the therapeutic effect in rats Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-09-12 Ezgi Eroğlu Çakmakoğlu, Adalet Çelebi, Muhammet Bahaeddin Dörtbudak, Hayati Yüksel, Aydın Keskinruzgar
Abstract The aim of this study was to evaluate the effect of ellagic acid on 5-fluorouracil-induced oral mucositis in rats. Twenty-four 6-month-old male Wistar albino rats were used. The rats were divided into three groups: Experimental Group I, Experimental Group II and Control Group. A superficial scratch was made on the mucosa of the right cheek pouch on the 3rd day and the 5th day. Oral mucositis
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Leber’s hereditary optic neuropathy: clinical and genetic analysis of Bulgarian patients Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-09-14 Sylvia Cherninkova, Boryana Zaharova, Kunka Kamenarova, Kalina Mihova, Slavena Atemin, Tihomir Todorov, Vasil Haykin, Alexander Oscar, Ivailo Tournev, Radka Kaneva, Albena Todorova
Abstract Leber’s hereditary optic neuropathy (LHON) is a rare maternally inherited disease caused by mutations in mitochondrial DNA (mtDNA) genes encoding subunits of complex I in the mitochondrial respiratory chain. The most common mutations causing LHON are G11778A, G3460A and T14484C, but there are also several less common mutations. LHON presents as acute or subacute bilateral visual loss, usually
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Serum sRAGE, sRANKL and osteoprotegerin in subgroups of rheumatoid arthritis patients: biomarkers associated with iron and disease status Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-09-12 Ginka Delcheva, Katya Stefanova, Teodora Stankova
Abstract Soluble receptors are important for the balance between ligands and their membrane receptors. Changes in the expression of soluble receptors are associated with human diseases. The aim of this study was to determine the serum levels of sRAGE, sRANKL and OPG in subgroups of rheumatoid arthritis (RA) patients according to the level of CRP and to assess the relationship of these parameters with
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Determination of expression level of AP1S1, CDK9, FIGF and HDAC11 genes in bladder tumors for aggressive phenotype characterization Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-09-09 Olga Antonova, Zora Hammoudeh, Zornitza Yordanova, Boris Mladenov
Abstract Bladder cancer holds a steady 10th place among oncological diseases. Follow-up and timely diagnosis of recurrence and progression of bladder cancer are still based on regular but outdated cystoscopy followed by cytological examination. To reduce the number of cystoscopy procedures, new and reliable biomarkers for predicting tumor behavior must be developed. The aim of this study was to confirm
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Improvement of the antibacterial and skin-protective performance of alcohol-based sanitizers using hydroglycolic phytocompounds Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-09-07 Tanya Rivera-Martinez, Benjamín Valdez-Salas, Jorge Salvador-Carlos, Margarita Stoytcheva, Roumen Zlatev, Ernesto Beltrán-Partida
Abstract The Coronavirus disease 2019 (COVID-19) outbreak increased the usage of hand rub disinfectants, resulting in skin damage. The World Health Organization (WHO) proposed hand rubs formulations incorporating glycerine due to its moisturizing effect; nonetheless, glycerine can reduce the antimicrobial activity of alcohol-based hand rubs. Here we demonstrated that incorporating hydroglycolic extract
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Awareness of healthcare professionals about risk minimization measures associated with COVID-19 vaccines: international cross-sectional study Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-09-06 Denitsa Panayotova, Maria Dimitrova, Assena Serbezova, Violeta Getova-Kolarova, Ilko Getov
Abstract The Coronavirus 2019 (COVID-19) pandemic caused the global healthcare to face one of the most significant challenges since the World Health Organization was founded over 70 years ago. The development and approval of marketing authorization for vaccines with the COVID-19 indication represents an opportunity to address this public health threat. The aim of this study is to analyze the awareness
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Relationship between bladder cancer and polymorphisms of OX40 (rs17568) and OX40L (rs1234313) genes in a Turkish population Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-08-28 Osman Bilen, Levent Verim, Cagatay Tosun, Omer Yuksel, Ulgen Sever, Dilara Sonmez, Mehmet Tolgahan Hakan, Ilhan Yaylim
Abstract The aim of this study was to investigate a possible association between the variations and effects of gene changes in the OX40/OX40L pathway and the risk of developing bladder cancer in a Turkish population. The study included 104 patients with bladder cancer and 97 healthy individuals. The distribution of OX40 (rs17568) and OX40L (rs1234313) polymorphisms was evaluated by polymerase chain
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Cost-effectiveness of therapeutic drug monitoring (TDM) of biologic treatment in rheumatic diseases: a narrative review Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-08-27 Konstantin Tachkov, Petya Nikolova, Rumyana Vitanska-Simeonova, Vladimira Boyadzhieva, Nikolay Stoilov, Guenka Petrova
Abstract This narrative review provides a brief horizon scanning on the available literature on cost-effectiveness of therapeutic drug monitoring (TDM) in rheumatology for biological medicines. The PubMed database was used to search for articles containing the key words ‘cost-effectiveness’ AND ‘therapeutic drug monitoring’ AND ‘rheumatology’ or ‘rheumatoid’ with no limits on year of publication. CHEERS
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Liquid biopsy: an innovative and reliable method for detecting not only somatic, but also germline mutations in patients with colorectal and non-small cell lung carcinoma Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-08-26 Nelly Miteva-Marcheva, Hristo Ivanov, Veselin Popov, Gabriela Raycheva, Zhanet Grudeva-Popova, Vili Stoyanova
Abstract Liquid biopsy is a non-invasive method of detecting cancer-related mutations from circulating cell-free DNA (cfDNA) and has emerged as a promising alternative to traditional tissue biopsies. However, its utility has so far been limited to the detection of somatic mutations in cancer cells. Our study examined germline mutations that are associated with pharmacogenetics in 19 patients diagnosed
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Association between endometrial microbiome and implantation success in women with frozen embryo transfer: results of a prospective cohort study Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-08-26 Dimitar Parvanov, Rumiana Ganeva, Margarita Ruseva, Maria Handzhiyska, Teodora Tihomirova, Stela Chapanova, Rada Staneva, Blaga Rukova, Maria Pancheva, Maria Serafimova, Dimitar Metodiev, Georgi Stamenov, Savina Hadjidekova
Abstract The aim of this prospective study was to compare the endometrial microbiome between pregnant and non-pregnant women after frozen embryo transfer (FET) with euploid embryos. Endometrial biopsies were collected from 30 women during the mid-luteal phase in a natural cycle. FET was performed with euploid embryos up to 3 months after the biopsy. Endometrial microbiota composition was analysed using
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Understanding the concept of Nattokinase use: a few years after beginning Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-08-24 Dimitar Maslarov, Desislava Drenska, Jane Maslarova-Gelov, Ivan Gelov
Abstract A number of scientific papers consider the specific and multifunctional pharmacological characteristics of Nattokinase, including those in the context of neuroprotection, neuroregeneration and neuroplasticity. We present the results from a 12-month observational study of 129 ischemic stroke and transient ischemic attack (TIA) patients treated with Nattokinase and unchanged antihypertensive
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Antibody dynamics and viral load in COVID-19: differences in mild, moderate and severe cases Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-08-24 Kim Ngoc, Iva Trifonova, Iva Christova, Magdalena Baymakova, Teodora Gladnishka, Anelia Zasheva, Maria Pishmisheva-Peleva, Elena Dragusheva, Vladislava Ivanova, Kamen Plochev, Raynichka Mihaylova-Garnizova
Abstract In this study, we investigated the potential association between the severity of COVID-19 (coronavirus disease 2019) and nasopharyngeal viral loads or serum antibody levels and evaluate the temporal dynamics of antibodies against SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2). Using ELISA (enzyme-linked immunosorbent assay), we determined the presence of IgA, IgM and IgG antibodies
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In silico analysis of Hsp70 genes in Ctenopharyngodon idella and their expression profiles in response to environmental stresses Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-08-11 Tianxiang Zhang, Feng Chen, Manman Li, Legang Jing, Peixian Luan, Guo Hu, Yongjun Shu
Abstract Heat shock protein 70 (Hsp70) is a crucial member of the Hsp family, which is present in many animals, and acts as a chaperone to protect the organism from damage caused by various environmental stresses, particularly unfavorable temperatures. In this study, we used homologous gene search and domain analysis to identify sixteen Hsp70 genes (named as CiHSP genes) from the genome of grass carp
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GmBBM7 promotes callus and root growth during somatic embryogenesis of soybean (Glycine max) Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-07-30 Runnan Zhou, Yujing Zhao, Peng Cheng, Binshuo Zhang, Zhen Liu, Sihui Wang, Haibo Li, Qingshan Chen, Ying Zhao, Sinan Li, Xiaoxia Wu
Abstract The BABY BOOM (BBM) subfamily of the AP2/ERF transcription factor family is the main regulator of totipotency in plant cells and plays an important role in regulating cell proliferation and plant growth and development. Previously, we identified GmBBM7, a key gene of the soybean BBM gene subfamily (GmBBM family). In this study, we aimed to analyze its molecular characteristics and role in
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Comparative transcriptome analysis of two varieties of common bean (Phaseolus vulgaris L.) to identify candidate drought resistance genes Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-07-24 Shaowen Zheng, Dan Zhou, Tingting Liu, Tianhong Wang, Xijiao Zhai, Guoming Xing, Meilan Li
Abstract In this work, we studied the physiological, biochemical and molecular mechanisms underlying the response to polyethylene glycol (PEG) in a drought-resistant variety ‘HZYTJ’ and the sensitive variety ‘WZWJD’ of common beans. The two bean varieties were subjected to a 10% PEG simulated drought stress treatment, and the resultant physiological and biochemical characteristics of the leaves and
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High quality RNA extraction from Pseudomonas aeruginosa and other bacteria with a novel rapid and cost-effective method Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-07-19 Ivan Stoikov, Ivan Nikolaev Ivanov, Deyan Donchev, Elina Dobreva, Rumyana Hristova, Stefana Sabtcheva
Abstract High-quality mRNA extraction is essential for gene expression assays. In this study, we developed a rapid method (20 min) named FACTS for the extraction of intact RNA from Pseudomonas aeruginosa and compared its performance to established rapid techniques like RNAsnap and CiAR. The RNA integrity, yield, purity and presence of residual genomic DNA were adopted as assessment criteria. Multiple
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Comparative study of the bond strength of ZirCAD Multi and ZirCAD Prime ceramics with different types of cements Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-07-14 Ivan Gerdzhikov, Elka Radeva, Todor Uzunov
Abstract Zirconia ceramic is a modern material used for different types of prosthetic constructions, characterized by high mechanical resistance and aesthetics. The main difficulties in treatment are related to ensuring a stable and durable bond between the ceramic and dentin. This study examined the bonding strength of ZirCAD Multi (3rd generation) and ZirCAD Prime (5th generation) ceramics when using
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Oligo-dT anchored cDNA-SRAP and cDNA-SCoT aided identification of transcripts differentially expressed during the early stages of recovery of resurrection plant Haberlea rhodopensis Friv. from freezing-induced desiccation Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-07-06 Elena Kumanova, Gergana Mihailova, Elena Georgieva Todorovska, Katya Georgieva, Stefan Tsonev, Nikolai Kirilov Christov
Abstract Resurrection plants are a unique group of flora showing an extraordinary ability to survive extreme water shortages. Among them, the Bulgarian endemic plant Haberlea rhodopensis Friv. has been used as a model to study the mechanisms underlying desiccation tolerance induced by water and low temperature stress. Although extensive transcriptome data, including two RNAseq experiments, are available
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Conducting clinical trials in five Eastern European countries (EU-EECs) with a focus on Bulgaria Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-07-02 Tatyana Benisheva, Dimitar Milkov, Valentin Kopanarov, Ivaylo Ivanov, Dimitar Dimitrov, Veselina Todorova, Nigyar Dzhafer, Iva Chavkova, Lyubina Todorova, Lena Gebert
Abstract Data for ongoing and completed clinical trials (Phases I-III) were extracted from the European Union Clinical Trials Register for five EU-EECs (Bulgaria, Hungary, Poland, Romania and Slovakia) (from January 1, 2012 to September 30, 2022). Based on the almost 11-year period Poland (n = 4134) and Hungary (n = 3845) represent the largest share of trials, with over 250–300 ongoing and completed
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Genome sequence of Bacillus velezensis LZN01 inhibiting Fusarium oxysporum f. sp. niveum and producing myriocin Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-06-23 Jiale Hu, Zhigang Wang, Weihui Xu
Abstract The bacterial strain LZN01 is a potential biocontrol agent that produces myriocin and inhibits the growth of Fusarium oxysporum f. sp. niveum (Fon). To characterize strain LZN01, the Illumina HiSeq + PacBio sequencing platform was used to sequence its genome. Unicycler software was used to perform genome assembly and antiSMASH to predict gene clusters responsible for secondary metabolite biosynthesis
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WRKY gene family of Licorice (Glycyrrhiza uralensis): identification and expression analysis in response to abiotic stress Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-06-20 Rui Li, Jing Gao, Nan Wang, Yonggang Yan, Gang Zhang, Jiakun Yan
Abstract WRKY transcription factors (TF) control a variety of biological processes in higher plants, including abiotic and biotic stress responses. WRKY genes have been identified and functionally characterized in many plant species during the past ten years, but despite this progress, few investigations have focused on this TF family in licorice (Glycyrrhiza uralensis Fisch.). This study identified
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Insights into effects of sodium phytate on gut microbiome of mice by high-throughput sequencing Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-06-09 Lijuan Wu, Xiaoqing Lao, Yazeng Wu, Jing Zhang, Manyu Liang, Yanhong Yang, Xiaowei Gao
Abstract Phytate, as the main phosphorus store in plant foods, is largely unavailable to monogastric animals, including humans, and has both adverse anti-nutritive and beneficial therapeutic effects for its consumers. The contradictory nature of phytate requires more caution when applying it. Administered phytate directly interacts with and affects gut microbiota, which has essential roles in host
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Astragalus glycyphyllos L.: antiviral activity and tablet dosage formulation of a standardized dry extract Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-06-09 Aleksandar Shkondrov, Anton Hinkov, Venelin Cvetkov, Kalina Shishkova, Daniel Todorov, Stoyan Shishkov, Ivan Stambolov, Krassimira Yoncheva, Ilina Krasteva
Abstract Astragalus glycyphyllos L. is widely used in Bulgarian folk medicine. Extracts from this plant have antiproliferative, antitumor and immune stimulating effects in vitro and in vivo. The aim of this study was to examine the potential antiviral activity of a standardized extract from the aerial parts of the plant and to formulate it in a suitable dosage form. A dry extract was obtained, and
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Inhibitory potential of phytochemicals on five SARS-CoV-2 proteins: in silico evaluation of endemic plants of Bosnia and Herzegovina Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-06-07 Tarik Corbo, Abdurahim Kalajdzic, Dzenita Omerkic, Farah Catic, Naris Pojskic, Kasim Bajrovic
Abstract The outbreak of the coronavirus disease 2019, caused by the SARS-CoV-2 virus, has prompted global health concerns. In response, researchers have been conducting investigations on active compounds in plants that may hold the potential to inhibit the proliferation of the virus. The aim of this study was to simulate and predict structural interactions of selected compounds isolated from 28 endemic
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Electrospun mini-MiSp spidroin/poly(L-lactide-co-ε-caprolactone) nanofibrous scaffolds for ARPE-19 cells Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-06-04 Changjun Liu, Ke Yi, Zheyang Zhang, Qinyu Li, Huimin Li, Cong Qu, Mengke Chen, Kelan Jin, Er Meng
Abstract Age-related macular degeneration, a type of retinal degenerative disease, is the primary cause of severe visual impairment and irreversible blindness in people older than 50, due to age-related deterioration of the photoreceptors and the retinal pigment epithelium (RPE), which could be restored by cell therapy. However, the success of cell therapy depends on biocompatible scaffolds, which
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Refractive results after cataract surgery in patients: calculation with Holladay II and Barrett Universal II formulas Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-05-21 Tanislava Kirova, Irina Kuneva, Luchezar Vojnov
The refractive result is a criterion for the quality of surgical correction of cataract. The aim of this study was to analyze the refractive results obtained in calculating the optical power of int...
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Platelet polymorphism rs5918T > C in the integrin B3 gene modulates comorbidities in patients with psoriasis Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-05-17 Borislav Dimitrov, Galya Georgieva, Klementina Gospodinova, Pencho Tonchev, Dimitar Gospodinov, Galya Stavreva, Regina Komsa-Penkova
Abstract Psoriasis Vulgaris is a complex multifactorial dermatological disease, with various genetic and environmental factors implicated in the onset and progression of the disease and comorbidities. Cardiovascular disease (CVD) and metabolic syndrome are essential psoriasis comorbidities that suggest a potential hypercoagulable background of the disease. To better understand the link between psoriasis
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Relationship between Vitamin D status and microbiome changes in Bulgarian patients with type 2 diabetes mellitus Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-05-15 Milena Velizarova, Vesselina Yanachkova, Tzenka Boneva, Silva Giragosyan, Ivelina Mihaleva, Pavlina Andreeva-Gateva, Dobrin Svinarov, Ivanka Dimova
Abstract The human microbiome plays a very important role in the normal functioning of both metabolic and immune systems. Changes in gut microbiota (dysbiosis) possibly contribute to the development of metabolic and autoimmune diseases. There is close association between the human gut microbiome and Vitamin D metabolism. The objective of our study was to characterize the composition of the gut microbiome
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Intracranial aneurysm with poor topography embolized by a coil guiding catheterization technique Biotechnol. Biotechnol. Equip. (IF 1.4) Pub Date : 2023-05-14 Hantao Yang, Liangliang Yang, Zejun Jia, Puyuan Zhao, Jinlong Huang, Zhigang Yang
Abstract Intracranial aneurysms with poor topography are still difficult and dangerous to be catheterized during endovascular procedures. Here we report a novel coil guiding catheterization technique which could be safe and helpful. Seven aneurysms of 5 cases were embolized with the novel coil guiding catheterization technique in Zhongshan Hospital, Fudan University, from January 2017 to March 2019