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The Effect of Structural Characteristics of Deproteinized Spongy Bone on Activity of Adipose Tissue Mesenchymal Stromal Cells Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-03-16 L. A. Cherdantseva, E. A. Anastasieva, M. N. Egorikhina, D. Ya. Aleynik, A. E. Medvedchikov, Yu. P. Sharkeev, I. A. Kirilova
We studied the effect of structural properties of deproteinized spongy bone (DSB) on functional activity of adipose tissue mesenchymal stromal cells of (MSC) for the potential use of these materials as components of a combined tissue-engineered construct. The porosity of the structure of DSB samples and the pore size promote MSC adhesion, migration, and proliferation on their surface and in the depth
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Quantitative Changes in Pro-Oncogenic and Tumor-Suppressing MicroRNA in the Regional Lymph Node during Photodynamic Therapy of Experimental Breast Cancer Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-03-16 A. V. Kabakov, O. V. Kazakov, A. F. Poveshchenko, V. N. Cherkas
We studied quantitative changes in microRNAs in the axillary lymph node (regional lymph node of the mammary gland) and in the breast tumor tissue. The quantity of pro-oncogenic microRNAs (miR-21 and miR-221) in the regional lymph node decreased in the group after photodynamic therapy of the breast cancer. At the same time, the quantity of pro-oncogenic miR-27a remained decreased in this group, as well
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Dynamics of Changes in the cAMP/cGMP Concentration Ratio in the Thymus and Spleen of Laboratory Mice during Vaccination against Plague and Tularemia against the Background of Immunomodulation Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-03-16 V. I. Dubrovina, O. V. Yur’eva, A. B. Pyatidesyatnikova, T. P. Starovoitova, S. V. Balakhonov
Vaccine strains Yersinia pestis EV NIIEG at a dose of 103 CFU and Francisella tularensis 15 NIIEG at a dose of 102 CFU induced changes in the concentration of cyclic nucleotides in the thymus and spleen of white mice. Antigen-induced changes in the cAMP/cGMP ratio in immunocompetent organs had a phase or oscillatory character, which seems to be related to the regulation of postvaccination immunoreactivity
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Immunohistochemical Analysis of Mitochondrial Dynamics in Different Zones of the Hippocampus during Experimental Modeling of Alzheimer’s Disease Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-03-16 T. I. Baranich, K. A. Skvortsova, D. N. Voronkov, A. V. Egorova, A. S. Averchuk, M. V. Ryazanova, A. V. Stavrovskaya, V. V. Glinkina, A. B. Salmina, V. S. Sukhorukov
We performed a comparative assessment of the immunohistochemical distribution of markers of mitochondrial fission (Drp-1), mitochondrial fusion (Mfn-2), and mitochondrial biogenesis (PGC-1α) in pyramidal neurons of different zones of the hippocampus in mice with intrahippocampal administration of β-amyloid peptide 25-35. The most pronounced changes in the dynamics associated with a decrease in the
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Association of Polymorphic Variants of TCF7L2 and PPARG Genes with Metabolic Markers in Patients with Early Disorders of Carbohydrate Metabolism Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-03-16 D. N. Isakova, S. N. Kolomeichuk, Yu. A. Petrova, M. V. Lyapina, I. A. Troshina, K. A. Voronin, I. M. Petrov
We performed complex analysis of the association of polymorphic variants rs7903146 of the TCF7L2 gene and rs1801282 of the PPARG gene with metabolic parameters, insulin resistance, and β-cell function in a group of patients with early signs of carbohydrate metabolism disturbances in a sample of Tyumen citizens. The study group consisted of 64 people (39 women, 25 men) aged 40-70 years. The distribution
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Expression of TLR2, IL-1β, and IL-10 Genes as a Possible Factor of Successful or Pathological Aging in Nonagenarians Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-03-16 S. O. Lukyanova, O. V. Artemyeva, I. D. Strazhesko, E. D. Nasaeva, V. V. Grechenko, L. V. Gankovskaya
The expression of the gene of pattern recognition receptor TLR2, proinflammatory cytokine IL-1β, and anti-inflammatory cytokine IL-10 was analyzed in the peripheral blood of nonagenarians (n=219; mean age 92.1 years, 77 men and 142 women) in comparison with healthy young donors (n=24; mean age 22.5 years, 16 women and 8 men). Nonagenarians were interviewed, medical records were analyzed, and a comprehensive
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Structural Changes in Rat Hepatocyte Nucleolus under Nucleolar Stress Caused by Hypothermia Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-03-16 I. P. Bobrov, A. Yu. Dolgatov, A. V. Lepilov, N. A. Korsikov, E. S. Dolgatova, M. G. Klinnikova, E. L. Lushnikova
Structural changes in rat hepatocyte nucleoli were studied during deep hypothermia simulated by immersion in water at 5°C for 40 min (ambient air temperature 7°C). In comparison with the control, phenomena of nucleolar stress occurred in rats during hypothermia: the number of fibrillar centers (FC) per nucleus (by 1.7 times) and per nucleolus (by 1.6 times), nucleolonemal nucleoli per nucleus (by 2
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DNA Methylation of a Group of Long Non-Coding RNA Genes at Different Stages of Ovarian Cancer Dissemination Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-03-16 A. M. Burdennyy, E. A. Filippova, S. S. Lukina, N. A. Ivanova, I. V. Pronina, V. I. Loginov, T. P. Kazubskaya, N. E. Kushlinskii, E. A. Braga
There are three types of metastases in ovarian cancer: lymphogenous, hematogenous, and peritoneal. Dissemination of the tumor in the peritoneum is directly related with the development of ascites and a poor prognosis. The purpose of this study is to determine changes in the methylation level of a group of long non-coding RNA (lncRNA) genes at different stages of ovarian cancer progression. The methylation
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Effect of Infrared and Green Photomodulation Exposure on the Number of Active Myosatellite Cells in Regenerating Muscles Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-03-16
Reparative properties of infrared laser exposure are well known, but the effects of green laser light are little studied. We analyzed the effects of short (60 sec) and longer (180 sec) exposure to infrared (980 nm) and green (520 nm) laser on the number of activated myosatellite cells in the regenerating m. gastrocnemius of Wistar rats after infliction of an incision wound. Histological preparations
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The Level of Sex Hormones and Corticosterone in Female Rats during Modeling of Visceral Obesity, Subsequent Physical Activity, and Normalization of the Diet Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-03-16 T. A. Mityukova, A. A. Basalai, O. E. Poluliakh, M. A. Darenskaya, L. V. Rychkova, S. I. Kolesnikov, N. V. Semenova, L. I. Kolesnikova
The effects of diet-induced visceral obesity and non-drug options of its correction on the level of sex hormones and corticosterone were evaluated in 84 female Wistar rats. During stage I, the rats received either a standard diet (STD) or a high-calorie diet (HCD) for 8 weeks. During stage II, the animals were divided into subgroups depending on obesity correction: without correction (STD control and
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Age-Related Features of the Response of Cancer Stem Cells and T Cells in Experimental Lung Cancer Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-03-16 O. V. Pershina, N. N. Ermakova, A. V. Pakhomova, E. S. Pan, L. A. Sandrikina, M. A. Zhukova, L. V. Kogai, A. M. Dygai, E. G. Skurikhin
The responses of tumor stem cells and various populations of CD4 and CD8 T cells of young and aged C57BL/6 mice were studied in a lung cancer model. Using Lewis lung carcinoma cell line, an orthotopic model of lung cancer was modeled. Cancer stem cells, circulating tumor cells, and various populations of CD4 and CD8 T cells in the blood and lung tissue were studied by cytometry. We revealed age-related
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Polymorphism in Genes Encoding Adaptor Proteins ST13 and STIP1 and the Risk of Ischemic Stroke: a Pilot Study Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-03-16 T. A. Stetskaya, A. B. Krapiva, K. A. Kobzeva, D. E. Gurtovoy, G. V. Komkova, A. V. Polonikov, O. Yu. Bushueva
Adaptor proteins stress induced phosphoprotein 1 (STIP1) and ST13 Hsp70 interacting protein (ST13) may play a crucial role in the pathophysiology of ischemic stroke through controlling protein folding, neuronal survival, and regulation of HSP70/HSP90. The present pilot study investigated whether tagSNPs in genes encoding ST13 (rs138335, rs138344, rs7290793, and rs138344) and STIP1 (rs4980524) are associated
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Cytotoxicity of Laser-Synthesized Nanoparticles of Elemental Bismuth Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-03-16 P. V. Shakhov, G. V. Tikhonowski, A. A. Popov, A. R. Iliasov, D. S. Petrunya, A. A. Lebedev, S. M. Klimentov, I. N. Zavestovskaya, A. V. Kabashin
High X-ray absorption combined with photothermal properties make bismuth nanoparticles (Bi NP) a promising agent for multimodal cancer theranostics. However, the synthesis of Bi NP by the “classical” chemical methods has numerous limitations, including potential toxicity of the produced nanomaterials. Here we studied in vitro toxicity of laser-synthesized Bi NP coated with Pluronic F-127 on mouse fibroblast
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Ratios between the Levels of IL-18, Free IL-18, and IL-1β-Binding Protein Depending on the Severity and Outcome of COVID-19 Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-03-15 A. A. Korotaeva, E. V. Samoilova, N. V. Pogosova, A. G. Fesenko, S. V. Evchenko, F. N. Paleev
In 89 patients with COVID-19, the ratios between IL-18, free IL-18, and IL-18-binding protein (IL-18BP) were analyzed depending on severity and outcome of the disease. At admission to the hospital, the levels of IL-18 and free IL-18 were significantly higher than 3 months after discharge from the hospital, the levels IL-18BP of being almost the same. In patients with more severe lung injury (computed
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The Role of δ2-Opioid Receptors in the Regulation of Tolerance of Isolated Cardiomyocytes to Hypoxia and Reoxygenation Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-03-15
Hypoxia (20 min) and reoxygenation (30 min) were simulated on isolated rat cardiomyocytes to evaluate the cytoprotective effect of selective δ2-opioid receptor agonist deltorphin II, opioid receptor antagonist naloxone methiodide, μ-opioid receptor antagonist CTAP, κ-opioid receptor antagonist nor-binaltorphimine, ε1-opioid receptor antagonist BNTX, and δ2-opioid receptors naltriben. Deltorphin II
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Evaluation of Collagenase Activity from Crab Hepatopancreas in Different Model Systems Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-03-15 N. G. Plekhova, I. N. Chernenko, D. V. Korolev, E. P. Kozlovskaya, A. K. Stepanyugina, O. V. Shevchenko, P. S. Dmitrenok, V. B. Shumatov
The effect of Kamchatka crab hepatopancreas containing three collagenolytic isoenzymes Collagenase KK and proteinases of Streptomyces lavendulae on metabolic activity and cell death were carried out on in vitro models. It was shown that changes in the protein structure under the influence of Collagenase KK occurred earlier than under the effect of bacterial proteinases. At the same time, activity of
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Effect of IQ-1 on the Infarct Size and the Parameters of Cardiodynamic Indicators in the Acute Period after Myocardial Ischemia/Reperfusion in Rats Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-03-15
The effect of a new JNK inhibitor IQ-1 (11H-indeno[1,2-b]quinoxalin-11-one oxime) was studied in male Wistar rats in a model of acute myocardial ischemia/reperfusion. Area at risk and myocardial infarct zones were studied in two series of experiments: 16 h after a single dose of IQ-1 (25 mg/kg intraperitoneally during cardiac ischemia) and on day 5 after its course administration (25 mg/kg intraperitoneally
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Microalbuminuria in Rats Treated with D-Nitroarginine Methyl Ether Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-03-15
Microalbuminuria is an early symptom and prognostic marker of the progression of renal pathology. The analysis of the role of anionic components of the renal glomeruli in the albumin retention and the development of a model of minimal changes in the glomerular filter leading to the appearance of microalbuminuria are relevant. The effect of organic cations D-arginine methyl esters (D-AME) and D-nitroarginine
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Characterization of Risk Factors for Modeling of Type 2 Diabetes Mellitus Induced by a High-Fat Diet in C57BL/6 Mice Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-03-15 N. A. Borozdina, E. R. Shaikhutdinova, G. A. Slashcheva, N. A. Goryacheva, A. V. Zamyatina, E. S. Sadovnikova, I. A. Pakhomova, V. M. Pavlov, N. A. Perepechenova, M. S. Severyukhina, A. Yu. Fedotova, D. V. Popkova, I. N. Gladkikh, E. V. Leichenko, I. A. Dyachenko
Type 2 diabetes mellitus develops due to a combination of genetic and environmental factors. C57BL/6 mice prone to obesity and leptin resistance were kept on a high-fat diet for 21 weeks. The animals showed a significant increase in fasting and postprandial glucose levels and body weight, the development of insulin resistance, and by week 18, an increase in the serum TNFα level. Metformin therapy at
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Influence of a Decrease in Blood Viscosity on Arterial Pressure in Normotensive and Spontaneously Hypertensive Rats Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-03-15 A. V. Sidekhmenova, O. I. Aliev, A. M. Anishchenko, O. I. Dunaeva, O. A. Ulyakhina, M. B. Plotnikov
We investigated the effect of a decrease in blood viscosity on the mean BP during isovolumic hemodilution and vasodilating activity of the endothelium in normotensive Wistar rats and spontaneously hypertensive rats (SHR). Blood viscosity was reduced by isovolumic hemodilution (replacement of 10% of circulating blood with an equal volume of plasma). Hemodilution caused the same reduction in blood viscosity
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Morphofunctional State of the Ovaries in Rats with Experimental Model of Functional Cysts and Their Treatment with Gonadotropin-Releasing Hormone Antagonist Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-02-12 O. S. Timofeeva, S. V. Logvinov, I. A. Petrov, O. A. Tikhonovskaya, Yu. G. Samoilova, Zh. F. Gaifulina, L. R. Mustafina, M. S. Petrova, A. A. Zhdankina, I. G. Kutsenko, D. A. Kudlay, K. A. Sidorenkova
The morphofunctional features of the ovaries were evaluated in rats with functional ovarian cysts model treated with gonadotropin-releasing hormone antagonist. Administration of the antagonist significantly (p=0.009) reduced the number of cysts and the growth of follicles in the ovaries. The obtained results attest to a possibility of successful treatment of functional ovarian cysts with gonadotropin-releasing
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Hemostasis System in Patients with Schizophrenia and Schizophrenia Spectrum Disorders Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-02-12
Hemostasis system in patients with schizophrenia and schizophrenia spectrum disorders was examined using “Fibrinodynamics” technology that allows evaluating the overal coagulation, fibrinolysis, and hemostasis potentials. All indices, except for the parameter characterizing the hemostasis balance, were statistically higher (p<0.0001) in patients than in healthy volunteers (control). In most patients
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Effect of Gonadotropin-Releasing Hormone Antagonist on the Expression Patterns of Insulin-Like Growth Factor 1, Androgen Receptor, and Luteinizing Hormone Receptor in an Experimental Rat Model of Functional Ovarian Cysts Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-02-12 O. S. Timofeeva, S. V. Logvinov, I. A. Petrov, O. A. Tikhonovskaya, Yu. G. Samoilova, Zh. F. Gaifulina, L. R. Mustafina, M. S. Petrova, A. A. Zhdankina, I. G. Kutsenko, D. A. Kudlay, K. A. Sidorenkova
We studied the expression of insulin-like growth factor 1 (IGF-1), androgen receptor (AR) and luteinizing hormone receptor (LHR) in the ovaries under the conditions of the modeling and subsequent treatment of functional ovarian cysts with gonadotropin-releasing hormone antagonist (ant-GnRH). The intensity of IGF-1, LHR, and AR expression in the generative elements of rat ovaries changed under conditions
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Expression and Significance of BCCIP and Glutathione Peroxidase 4 in Clear Cell Renal Cell Carcinoma Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-02-12 Yao Liu, J. Liu, C. Liu, J. Jin, Yu Liu
In this retrospective study involving 112 patients with clear cell renal cell carcinoma (ccRCC), we analyzed clinical significance and prognostic value of the expression of BCCIP protein interacting with BRCA2 and CDKN1A and glutathione peroxidase 4 (GPX4). The expressions of mRNA and the corresponding proteins were evaluated using reverse transcription PCR and immunohistochemistry. In comparison with
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The Pattern of Granuloma Formation in the Liver of Rats as a Reflection of the Mechanism of Internalization of Submicron Silicon Particles Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-02-12
A morphological analysis of the liver of Wistar rats was performed 2 months after a single intravenous injection of porous silicon particles of different sizes (60-80, 250-300, and 500-600 nm; 2 mg/ml, 1 ml). Histological, immunohistochemical, and electron microscopic methods showed the development of CD68+ granulomas in all experimental groups. Injection of 60-80-nm porous silicon particles led to
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Features of Cytokine and VEGFA Gene Expression Modified with SARS-CoV-2 Virus in an In Vitro Experiment (Using the Example of the SARS-CoV-2 Vaccine Antigen) Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-02-12 K. G. Starkova, O. V. Dolgikh, I. N. Alikina, O. A. Kazakova, N. A. Nikonoshina, V. B. Alekseev
The influence of SARS-CoV-2 antigen on the cytokine-producing function of immune cells was studied. We observed suppression of the production of proinflammatory cytokines by 11-46% relative to the spontaneous level under the influence of SARS-CoV-2 antigen vaccine simulator, as well as when it was co-administered with cortisol (IL-6 by 1.8 times and IFNγ by 1.57 times) compared with control samples
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Noninvasive and Invasive Study of the Pumping Function of Rat Heart in Myocardial Infarction Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-02-12
The cardiohemodynamics was studied 2 and 4 weeks after myocardial infarction modeling in Wistar rats. We compared the data obtained by echocardiography (echoCG) and catheterization of the left ventricle. The myocardial infarction was modeled by ligation of the left anterior descending coronary artery. EchoCG and the left ventricle catheterization were performed before and after myocardial infarction
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Effects of Gly-His-Lys-D-Ala Peptide on Skin Wound Regeneration Processes Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-02-12
We evaluated the effects of the Gly-His-Lys-D-Ala peptide in a dose 0.5 μg/kg on skin wound regeneration in male Wistar rats (n=80) after initial surgical debridement when administered intracutaneously around the site of injury. Histological (severity of the inflammatory reaction, formation of granulation tissue, and epithelialization terms) and morphometric (number of fibroblastic cells, macrophages
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Stress-Tolerant Dormant Bacterial Forms: Biological and Ultrastructural Properties of Moraxella catarrhalis and Kocuria rhizophila Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-02-12
Dormant forms of causative agents of healthcare-acquired infections Moraxella catarrhalis and Kocuria rhizophila have been obtained. Dormant forms cells retained viability during long-term storage (≈107 CFU/ml after 2 months) under provocative conditions (lack of nutrient sources; temperature 20°C, oxygen access) were characterized by heat resistance, and acquired special ultrastructural organization
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Influence of a Passive Tilt Test on the Proteomic Composition of the Blood of Healthy Humans Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-02-12 D. N. Kashirina, L. Kh. Pastushkova, A. S. Kononikhin, A. G. Brzhozovskiy, I. M. Larina
In order to identify changes in the blood proteome of healthy volunteers after passive tilt test carried out on day 19 of head-down bed rest, a chromato-mass-spectrometric analysis of samples of dried blood spots was carried out. It was revealed that the body’s response to the tilt test was characterized by a decrease in the level of HDL and kininogen-1. After the tilt test, we observed an increase
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Simulated Microgravity and Hypergravity Affect the Expression Level of Soluble Guanylate Cyclase, Adenylate Cyclase, and Phosphodiesterase Genesin Rat Ventricular Cardiomyocytes Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-02-12 V. M. Mitrokhin, O. V. Kamkina, A. G. Kamkin, A. S. Rodina, A. D. Zolotareva, V. I. Zolotarev, V. E. Kazansky, L. R. Gorbacheva, A. S. Bilichenko, S. A. Shileiko, M. I. Mladenov
Ion channels activity is regulated through soluble guanylate cyclase (sGC) and adenylate cyclase (AC) pathways, while phosphodiesterases (PDE) control the intracellular levels of cAMP and cGMP. Here we applied RNA transcriptome sequencing to study changes in the gene expression of the sGC, AC, and PDE isoforms in isolated rat ventricular cardiomyocytes under conditions of microgravity and hypergravity
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Peracute Myocardial Infarction: Features of the Influence of α2-Adrenoreceptor Stimulation on the Isolated Heart Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-02-12 A. M. Kuptsova, R. K. Bugrov, N. I. Ziyatdinova, T. L. Zefirov
We performed a comparative analysis of the effect of α2-adrenoreceptor stimulation on the performance of Langendorff-isolated heart from rats with experimental myocardial infarction in the acute stage and sham-operated animals (control). In animals with peracute myocardial infarction, different agonist concentrations (clonidine, 10-6 and 10-9 M) produced a multidirectional effect on the left-ventricular
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Changes of the Concentration of Short-Chain Fatty Acids in the Intestines of Mice with Different Types of Obesity Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-02-12 E. V. Krolenko, O. V. Kupriyanova, L. S. Nigmatullina, T. V. Grigoryeva, S. A. Roumiantsev, A. V. Shestopalov
We studied the production of short-chain fatty acids (SCFA) by the intestinal microbiota in mice with obesity caused by a diet and a genetic defect in the leptin receptor gene. In mice, intestinal contents were examined and SCFA were quantitatively assayed by gas chromatography. SCFA concentration in the intestinal contents of mice with alimentary obesity model was significantly lower in the first
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Comparative Analysis of Infarct-Limiting Activity of Peptide and Non-Peptide δ- and κ-Opioid Receptor Agonists during Heart Reperfusion In Vivo Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-02-10
A comparative analysis of the infarct-limiting activity of δ- and κ-opioid receptors (OR) agonists was carried out on a model of coronary occlusion (45 min) and reperfusion (120 min) in male Wistar rats. We used selective δ2-OR agonist deltorphin II (0.12 mg/kg), δ-OR agonists BW373U86 and p-Cl-Phe DPDPE (0.1 and 1 mg/kg), selective agonists of δ1-OR DPDPE (0.1 and 0.969 mg/kg), κ1-OR U-50,488 (0.1
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Assessment of Autophagy in Tumor Cells of Human Skin Melanoma of Different Stages Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-02-10 Yu.S. Taskaeva, A. Yu. Shatruk, A. I. Lomakin, N. P. Bgatova, S. A. Fursov
High levels of autophagy can increase the viability of tumor cells as well as their resistance to chemotherapy. Evaluation of the dynamics of autophagy processes at different stages of carcinogenesis can extend our understanding of melanoma pathogenesis to develop new therapeutic approaches. We performed a comparative study of tumor cell autophagy in stages II and III human skin melanoma. Tumor cells
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Sepsis-Induced Inhibition of Contractile Function of Lymphatic Nodes Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-02-10 M. E. Kosareva, A. V. Chivildeev, G. I. Lobov
Inflammation accompanies most pathological processes, while the lymphatic system takes part in both the development and resolution of inflammation. We studied the contractile function of rat lymph nodes after cecal ligation and puncture (CLP). In 24 h after CLP, the mesenteric lymph nodes were removed and placed in the myograph chamber. After CLP, the lymph nodes showed lower tension than lymph nodes
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Oxidative Damage of DNA, Proteins and C-Reactive Protein Parameters in Girls and Boys with Exogenous Constitutional Obesity Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-02-10 M. A. Darenskaya, L. V. Rychkova, S. I. Kolesnikov, N. V. Semenova, O. A. Nikitina, A. S. Lesnaya, L. I. Kolesnikova
The parameters of oxidative damage of DNA, proteins, as well as the parameters of the thiol-disulfide system and C-reactive protein in adolescent girls and boys with exogenous constitutional obesity (ECO) were evaluated. In girls and boys with obesity, the levels of 8-hydroxy-2’-deoxyguanosine (marker of DNA destruction) were higher than in controls. Evaluation of the activity of the thiol-disulfide
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An Individualized Approach to Skin Conductance Assessment during Execution of Tasks of Different Complexities Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-02-10 E. A. Kriklenko, A. V. Kovaleva, E. N. Likhomanova
Researchers use different approaches to analyze changes in skin conductance. At the same time, individual differences observed in both the initial values of skin conductance and response to stimuli do not allow comparing the responses of different subjects to the same impact. An individualized approach to the assessment of psycho-emotional stress has been developed using the real-time skin conductance
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Prognostic Significance of Tumor-Associated Inflammation in Renal Cell Carcinoma Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-02-10 O. V. Kovaleva, P. A. Podlesnaya, V. V. Mochalnikova, N. E. Kushlinskii, L. M. Khromykh, A. A. Kalinina, D. B. Kazansky, A. N. Gratchev
This study analyzed tumor-associated inflammation by assessing the expression of cyclophilin A (CypA) and TNF in samples of kidney tumors of various histological types. It was shown that different histological types of renal cell carcinoma differed by the expression of these proteins. Thus, the highest expression of CypA and TNF was observed in papillary and chromophobe kidney cancer, although no correlation
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Effect of Inducers and Inhibitors of the Keap1/Nrf2/ARE System on the Viability and Functional Activity of Model Neuronal-Like and Glial Cells Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-02-10 E. B. Menshchikova, A. V. Chechushkov, P. M. Kozhin, L. P. Romakh, A. E. Serykh, M. V. Khrapova, E. S. Petrova, N. V. Kandalintseva
On mouse neuroblastoma (Neuro-2a) and human glioblastoma (U-87 MG) cell lines, we studied the effect of inducers and inhibitors of redox-sensitive signaling system of the antioxidant-responsive element Keap1/Nrf2/ARE on the main processes that determine nerve cell viability and vital activity (proliferative activity, apoptosis, autophagy, and activation of the Keap1/Nrf2/ARE system). Inhibitors of
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A Validation Study of CD133 as a Reliable Marker for Identification of Colorectal Cancer Stem-Like Cells Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-02-10 C. Z. You, H. Xu, F. S. Zhao, J. Dou
Colorectal carcinoma (CRC) is maintained by putative colorectal cancer stem-like cells (CRC-CSCs) that are responsible for CRC metastasis and relapse. Targeting these CSCs can be an effective treatment of CRC. However, reliable identification of CRC-CSCs remains controversial due to the absence of specific markers. It is assumed that glycoprotein CD133 can serve as a useful marker for identification
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N-Terminal Fragment of Cardiac Myosin Binding Protein-C Increases the Duration of Actin—Myosin Interaction Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-02-10 S. R. Nabiev, A. M. Kochurova, L. V. Nikitina, E. A. Beldiia, A. M. Matyushenko, D. S. Yampolskaya, S. Yu. Bershitsky, G. V. Kopylova, D. V. Shchepkin
Cardiac myosin binding protein-C (cMyBP-C) located in the C-zone of myocyte sarcomere is involved in the regulation of myocardial contraction. Its N-terminal domains C0, C1, C2, and the m-motif between C1 and C2 can bind to the myosin head and actin of the thin filament and affect the characteristics of their interaction. Measurements using an optical trap showed that the C0-C2 fragment of cMyBP-C
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Neuroinflammation in Obese Children Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-02-10 Yu.G. Samoilova, M. V. Matveeva, L. V. Spirina, D. V. Podchinenova, O. A. Oleinik, D. E. Galyukova
Obesity is associated with chronic persistent inflammation due to a pool of tissue macrophages that can penetrate the blood—brain barrier and cause neuroinflammation. The analysis of the association of CD14+CD163+ monocytes in the peripheral blood with cognitive functions in 56 obese children (mean age 11.95 (9.45; 14.45) years) was carried out. The control group consisted of 10 children (mean age
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Different Patterns of Vascularization in Preinvasive States and at the Initial Stages of Invasive Growth of the Neoplasms Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-01-16 A. I. Karseladze
Tissue samples obtained during surgery from 90 patients with malignant neoplasms of various localizations were studied (the presence of precancerous dysplastic alterations and their transition to invasive cancer in the slides were obligatory condition). In addition to traditional histological methods, immunohistochemical reactions for detection of HIF-1α, GLUT1, CAIX, and CD31 were performed. At the
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The Effect of Original Russian Neurotropic Drugs on Organic Anion Transporting Polypeptides OATP1B1 and OATP1B3 Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-01-10 P. D. Erokhina, Yu. V. Abalenikhina, P. Yu. Mylnikov, A. V. Petrov, S. O. Ganina, E. A. Konyakhin, A. V. Shchulkin, E. N. Yakusheva
In experiments on HepG2 cells, we studied the effect of the original domestic neurotropic drugs omberacetam, fabomotizole, and ethylmethylhydroxypyridine succinate (EMHPS) (1-500 μM) on the activity and content of organic anion transporting polypeptides OATP1B1 and OATP1B3. It was shown that omberacetam (500 μM) increased the content of OATP1B1 and OATP1B3, fabomotizole did not affect the level of
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The Influence of Exosomes Derived from Mesenchymal Stem Cells on the Development of Fibrosis In Vitro Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-01-10
We studied the effect of exosomes derived from mesenchymal stem cells on the synthesis of collagen I and α-smooth muscle actin (α-SMA) by rat fibroblast culture. Exosomes were isolated from the verified culture of mesenchymal stem cells and also verified. Fibrosis was modeled using a fibroblast culture supplemented with recombinant TGF-β1 (5 ng/ml) and immunocytochemical analysis of the expression
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Expression of GAD65/67 and VGLUT2 in Mediobasal Nuclei of Rat Hypothalamus during Aging Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-01-09 P. A. Anfimova, L. G. Pankrasheva, A. I. Emanuilov, K. Yu. Moiseev, P. M. Maslyukov
The expression of glutamate decarboxylase GAD65/67, an enzyme of GABA synthesis, and vesicular glutamate transporter 2 (VGLUT2) in the arcuate, dorsomedial, and ventromedial nuclei of the hypothalamus of young (3 months), adult (12 months), and old male rats (24 months) was studied by Western blotting. In old rats, an increase in the expression of GAD65/67 in the arcuate and dorsomedial, VGLUT2 in
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Preclinical Study of Therapeutic Efficacy of a New Russian Radiopharmaceutical 177Lu-DOTA-PSMA Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-01-09 V. K. Tishchenko, O. P. Vlasova, A. A. Lebedeva, A. V. Fedorova, A. A. Pankratov, N. B. Morozova, K. A. Kuzenkova, E. D. Stepchenkova, P. V. Shegai, S. A. Ivanov, A. D. Kaprin
The therapeutic efficacy of a Russian radiopharmaceutical 177Lu-DOTA-PSMA was studied in vivo using male BALB/c nu/nu (nude) mice with prostate carcinoma 22Rv1 xenografts by tumor growth inhibition criterion. The mean tumor volumes in mice treated with 177Lu-DOTA-PSMA were significantly lower than in animals of the control group. There were no significant differences in the values of tumor growth inhibition
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Screening of Peptides that Specifically Binds to M3-M4 Extracellular Domain of Sodium Pump α1 Subunit and Analysis of Their Bioactivity In Vitro and In Vivo Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-01-09 Tianjiao Wen, Chan Li, Siying Li, Peipei Yu, Xiaoran Yu, Luya Li, Jingxia Cui
Interaction between ouabain (OUA) and Na+/K+-pump remains in the current focus of hypertension research. This study aimed to find an oligopeptide that would antagonize the inhibitory effect of endogenous OUA on Na+/K+-pump and examine its activity at the cellular and organism levels. To this end, Phage Random 12 Peptide Library was employed to screen for specific polypeptide ligands that interact with
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Ex Vivo Production of IL-1β and IL-10 by Activated Blood Cells of Wistar Rats with Different Resistance to Hypoxia after Systemic Inflammatory Response Syndrome Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-01-09 A. M. Kosyreva, D. Sh. Dzhalilova, I. S. Tsvetkov, M. A. Makarova, O. V. Makarova
We studied spontaneous and ex vivo activated cytokine production by blood cells of male Wistar rats with different resistance to hypoxia against the background of an LPS-induced systemic inflammatory response. In rats with low (LR) and high resistance (HR) to hypoxia, the number of leukocytes, granulocytes, and peripheral blood lymphocytes was determined, the levels of spontaneous and stimulated production
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Xenon-Induced Recovery of Functional Activity of Pulmonary Surfactant (In Silico Study) Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-01-09
To understand the nature of xenon-induced recovery of the functional activity of pulmonary surfactant during inhalation of a gas mixture of Xe/O2, the mechanisms of the ongoing processes were studied in silico. Impaired ability of pulmonary surfactant to maintain low surface tension preventing alveolar atelectasis occurs due to formation of aggregates of its phospholipids and a decrease in their lateral
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DNIC is a Structure Providing Specificity of Physiological Effects of NO Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-01-09 V. Yu. Titov, A. M. Dolgorukova, A. N. Osipov, I. I. Kochish
Metabolism of nitric oxide (NO) donors: dinitrosyl iron complexes (DNIC), nitrosothiols (RSNO), and nitroprusside was studied on a chick embryo model. The obtained results give reason to assume that DNIC constituting the main pool of nitroso compounds in the vast majority of tissues are NO donors immediately interacting with the physiological target of NO, and other NO donors can perform this function
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Lactoferrin Has a Protective Effect on Mouse Brain Cells after Acute Gamma Irradiation of the Head Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-01-09
We studied the effect of human lactoferrin on cells of the hippocampal dentate gyrus of 2-2.5-month-old male C57BL/6 mice after acute gamma irradiation of the head in a dose of 8 Gy from a 60Co source. Immediately after irradiation some animals received an intraperitoneal injection of human lactoferrin (4 mg/mouse). The appearance of TUNEL+ cells in the subgranular zone 6 h after irradiation was accompanied
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Biogenesis of Mitochondria in Multipotent Mesenchymal Stromal Cells in Patients with Acute Leukemia Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-01-09 A. V. Sadovskaya, N. A. Petinati, N. V. Sats, N. I. Drize, A. N. Vasil’eva, O. A. Aleshina, E. N. Parovichnikova
In patients with acute leukemia, not only normal hematopoiesis, but also bone marrow stromal microenvironment is damaged. Multipotent mesenchymal stromal cells (MSC) are essential for the formation and function of the stromal microenvironment. Analysis of changes in MSC is important for the development of new approaches to leukemia therapy. The metabolism of mitochondria in MSC, relative content of
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Analysis of the Morphology of Monocytes and Lymphocytes from COVID-19 Patients Using Low-Voltage Scanning Electronic Microscopy Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-01-09 A. A. Velmiskina, Yu. V. Nikitin, V. Yu. Mikhailovskii, S. V. Mosenko, A. Yu. Anisenkova, S. V. Apalko, N. N. Sushentseva, S. G. Scherbak, A. M. Ivanov, N. K. Galaktionov, O. V. Shneider, K. A. Kondratov
Severe course of COVID-19 is largely determined by hyperactivation of the immune system, or cytokine storm, in which immune cells (lymphocytes, monocytes, etc.) play a major role. Using low-voltage scanning electron microscopy, we studied the morphology of lymphocytes and monocytes during cytokine storm. Monocytes and lymphocytes were isolated by fluorescence sorting from the blood of healthy volunteers
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Effect of Lysine Acridone Acetate on the Level of Apoptosis and Expression of Apoptosis-Associated Proteins during Antioncogenic Therapy in Colorectal Cancer in Mice Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-01-09
We studied the mechanism of action of cytostatics with the addition of lysine acridone acetate to evaluate the possibility of its use for improving the effectiveness of antioncogenic therapy in colorectal cancer. In Nude mouse model, the level of apoptosis (TUNEL) and expression of proteins CD95, p53, Bcl-2, histone H3, and Ki-67 (immunohistochemistry) were assessed in primary tumor biopsy specimens
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Evaluation of the Effectiveness of Bone Grafting in Alveolar Ridge Augmentation Using the Two-Stage Splitting Technique Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-01-09 S. S. Edranov, N. Yu. Matveeva, S. G. Kalinichenko
Stimulation of neoosteogenesis is the main mechanism of osseointegration during installation of dental implants, bone tissue recession, and alveolar process augmentation in adentia. In experiments on miniature pigs, we used the technology of two-stage splitting of the ridge of the alveolar process of the mandible in combination with a xenograft that was placed between the fragments of the split bone
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Age-Related Features of the Early Period of Liver Regeneration after Partial Hepatectomy in Rats Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-01-09 E. S. Pan, N. N. Ermakova, O. V. Pershina, A. V. Pakhomova, M. A. Zhukova, L. A. Sandrikina, L. V. Kogai, S. A. Afanas’ev, T. Yu. Rebrova, V. A. Korepanov, S. G. Morozov, A. A. Kubitaev, A. M. Dygai, E. G. Skurikhin
Regenerative processes in the liver were studied in 3-month-old (young) and 9-month-old (aged) male Wistar rats on day 1 after 30 and 70% hepatectomy. Regardless of the resected liver volume, shifts in the biochemical parameters of the serum in aged rats were more pronounced than in young animals. After 30% hepatectomy, no age differences in the rate of hepatic regeneration were found, while after
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The Search for Diagnostic Criteria to Divide the Wistar Rat Population into Phenotypes during Modeling of Post-Traumatic Stress Disorder Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-01-09 M. V. Kondashevskaya, V. V. Aleksankina, K. A. Artem’eva
The levels of circulating hormones (corticosterone, testosterone, and leptin) and interleukins were studied in sexually mature male Wistar rats against the background of post-traumatic stress disorder (predator threat stress). It was found that in addition to the previously used anxiety index determined by animal behavior, the population of stressed individuals can be divided into stress-resistant
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The Influence of Adiponectin on Transport of Low-Density Lipoproteins through Human Endothelial Cell Monolayer In Vitro Bull. Exp. Biol. Med. (IF 0.7) Pub Date : 2024-01-09
The influence of adiponectin, a protein secreted by adipocytes, on the activation of transendothelial LDL transport, the initial event of atherogenesis, was studied. The addition of adiponectin to the cultured endothelial hybridoma EA.hy926 cells did not affect both basal and TNF-stimulated transendothelial transport of LDL. In addition, adiponectin affects neither expression levels of CAV1, SCARB1