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Morphofunctional State of the Large Intestine in Rats under Conditions of Restraint Stress and Administration of Peptide ACTH (4-7) -PGP (Semax) Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2021-01-18 M. V. Svishcheva, Ye. S. Mishina, O. A. Medvedeva, I. I. Bobyntsev, A. Y. Mukhina, P. V. Kalutskii, L. A. Andreeva, N. F. Myasoedov
We studied the effect of intraperitoneal administration ACTH(4-7)-PGP in doses of 5, 50, 150, and 450 μg/kg to Wistar male rats 12-15 min before modeling restraint stress on the morphofunctional state of the colon. In rats exposed to restraint stress, signs of atrophy and inflammatory reaction in the colon wall, changes in functional activity and number of mast cells, and increased serum level of corticosterone
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Age Peculiarities of Respiratory Activity and Membrane Microviscosity of Mitochondria from Rat Cardiomyocytes Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2021-01-16 T. Yu. Rebrova, V. A. Korepanov, S. A. Afanasiev
We studied respiratory activity and microviscosity of mitochondrial membranes from the left ventricular myocardium of 2-month-old (n=8) and 15-month-old (n=8) rats. The respiratory control (RC) during substrate phosphorylation was calculated as the ratio of oxygen absorption rates in the presence of ADP and after its utilization. In 2-month-old rats, RC was 4.66 (4.56; 4.71); in 15-month-old animals
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In Vivo Studies of a Liquid Embolic Compound Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2021-01-16 O. A. Legon’kova, A. V. Chupin, A. S. Ogannisian, A. V. Nikitina, V. V. Stafford, I. P. Savchenkova, A. B. Varava, S. V. Sapelkin, S. V. Pozyabin
A composition in the form of liquid polymer substance intended for embolization procedures was studied in in vivo experiment. The preparation was injected to rabbits into the femoral artery and abdominal aorta. The polymer composition exhibited properties previously demonstrated in vitro: strong adhesion to the vascular wall, high plasticity sufficient for embolization of the blood vessels, distal
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Effect of Long-Term Light Deprivation on α-Tocopherol Content in Rats during Ontogeny Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2021-01-16 I. V. Baishnikova, T. N. Ilyina, E. A. Khizhkin, V. A. Ilyukha, I. A. Vinogradova
We studied the effect of long-term light deprivation which began at different stages of ontogeny on the content of α-tocopherol in rats during the first 3 months of postnatal development. In the offspring postnatally exposed to constant darkness, the level of α-tocopherol in the liver, kidneys, heart, skeletal muscles, and lungs was significantly decreased at the early stages of postnatal ontogeny
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Experimental Extracellular Matrix of the Xenoperitoneum: Comprehensive Assessment of a New Biological Material for Plastic Surgery of Anterior Abdominal Wall Defects Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2021-01-16 L. V. Maxyatkina, R. M. Badyrov, N. T. Abatov, L. L. Akhmaltdinova
A new biological implant, extracellular matrix of bovine peritoneum, was developed and obtained for plastic surgery of the anterior abdominal wall defects. The material was implanted to rats into the anterior abdominal wall. The content of rejection markers (TNF, IL-2, C-reactive protein) and the morphological picture of the implantation zone at the early stages (30 days) after surgery were evaluated
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Effects of Zoniporide and BMA-1321 Compound on the Rate of Oxygen Absorption by Cardiomyocyte Mitochondria in Rats with Experimental Chronic Heart Failure Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2021-01-16 A. A. Spasov, N. A. Gurova, T. A. Popova, V. N. Perfilova, V. V. Vishnevskaya, M. V. Kustova, N. V. Ovsyankina, A. A. Ozerov
Uncoupling of respiration and ATP production by myocardial mitochondria was observed in rats with chronic isoproterenol intoxication (L-isoproterenol subcutaneously, 1 mg/kg, for 10 days) in comparison with controls (injected with the solvent). Inhibitors of NHE-1 zoniporide (1 mg/kg intraperitoneally, 13 days) and BMA-1321 compound (0.92 mg/kg intraperitoneally, 13 days) improved the mitochondrial
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Genetic Factors as the Basis of Sex Differences in Damage to Lung Endothelium and Regulation of Angiogenesis Cells in Modeling Pulmonary Emphysema in C57BL/6 Mice with Dyslipidemia and Hyperglycemia Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2021-01-16 E. G. Skurikhin, A. V. Pakhomova, O. V. Pershina, N. N. Ermakova, V. A. Krupin, E. S. Pan, L. A. Sandrikina, O. D. Putrova, M. A. Zhukova, I. V. Kurochkina, A. M. Dygai
We studied the formation of injuries in lung endothelium and the response of angiogenesis cells during modeling of pulmonary emphysema in male and female C57BL/6 mice with metabolic disorders. Hemodynamic disturbances and reduction in the area of the microvasculature caused by combined pathology in male mice were more pronounced than in females. Mobilization and migration of angiogenic precursors were
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Biocompatibility and Bioresorption of 3D-Printed Polylactide and Polyglycolide Tissue Membranes Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2021-01-16 E. V. Kim, Y. S. Petronyuk, N. A. Guseynov, S. V. Tereshchuk, A. A. Popov, A. V. Volkov, V. N. Gorshenev, A. A. Olkhov, V. M. Levin, A. B. Dymnikov, V. E. Rodionov, G. A. Tumanyan, S. G. Ivashkevich, A. P. Bonartsev, L. L. Borozdkin
We studied biocompatibility and bioresorption of 3D-printed polylactide and polyglycolide tissue membranes. Ultrasound microscopy and histological examination showed that membranes fabricated of a copolymer of lactic and glycolic acids in a mass ratio of 1:9 are bioresorbed and have good biocompatibility with soft tissues (connective tissue, adipose tissue, and epithelium). An important feature of
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Features of Remodeling of Prostate Stroma in Chronic Abacterial Prostatitis Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2021-01-16 G. A. Lapii, O. P. Molodykh, N. A. Abdullaev, M. A. Bakarev
We performed a complex morphological study of biopsy samples of prostate gland from 30 men (mean age 46±11 years) with chronic abacterial prostatitis who were exposed unfavorable anthropogenic factors. The ultrastructural heterogeneity of smooth muscle cells was shown, among which cells with destructive changes in organelles predominated, solitary cells with signs of secretory function were revealed
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The Specifics of Adrenergic Innervation of Lymph Nodes from Different Body Regions in Young, Mature, and Old Animals Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2021-01-16 G. A. Demchenko, S. N. Abdreshov, N. A. Akhmetbaeva, E. K. Makashev, B. A. Nurmakhanova, A. O. Balkhybekova, A. M. Kalekeshov
Adrenergic innervation in the tissue of the cervical, mesenteric, and popliteal lymph nodes in rats of different age groups (from 2-45 days to 24 months) was studied using the specific fluorescence-microscopic imaging of catecholamines. Adrenergic innervation in the lymph nodes of rats is present from the first days of life, but it develops and complicates during postnatal ontogeny and is fully formed
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Comparative Morphological Characteristics of the Results of Implantation of Decellularized and Recellularized Porcine Skin Scaffolds Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2021-01-16 A. S. Sotnichenko, I. V. Gilevich, K. I. Melkonyan, Y. A. Yutskevich, T. V. Rusinova, A. V. Karakulev, S. B. Bogdanov, V. A. Aladina, Yu. A. Belich, S. E. Gumenyuk, D. I. Ushmarov, I. M. Bykov, A. N. Redko, V. A. Porhanov, S. N. Alekseenko
The tissue reaction of pig skin to implantation of decellularized and recellularized dermal matrices on a formed wound defect was evaluated by histological methods on days 2, 5, 8, 16, and 20 after surgery. Differences in tissue response to different matrices were identified. In experimental wounds coated with decellularized dermal matrices, we observed the formation of a scar tissue, which required
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Changes in the Lipid Peroxidation—Antioxidant Protection System in Women with Ovarian Hyperandrogenism at Different Periods of Reproductive Age Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2021-01-16 L. I. Kolesnikova, O. V. Krusko, L. V. Belenkaya, L. F. Sholokhov, L. A. Grebenkina, N. A. Kurashova, S. I. Kolesnikov
We studied changes in LPO processes and antioxidant protection system in women with ovarian hyperandrogenism at various periods of reproductive age 18-45 years. An age-related decrease in total antioxidant activity and accumulation of secondary LPO products, ketodiens and coupled trienes, were found; these changes were accompanied by an increase in the level of superoxide dismutase and a decrease in
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Biodistribution of 68 Ga-NODA-Aminoglucose in Intact and Tumor-Bearing Mice Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2021-01-16 V. K. Tishchenko, V. M. Petriev, A. A. Mikhailovskaya, P. V. Shegai, S. A. Ivanov, A. D. Kaprin
We studied the biodistribution of 68Ga-NODA-aminoglucose (68Ga-NODA-AG) in normal mice and mice with experimental model of colon adenocarcinoma tumor. It was shown that 68Ga-NODA-aminoglucose was retained in the tumor for 3 h after injection and demonstrated high level of accumulation in the tumor. Rapid clearance of radioactivity from other organs was observed. The results suggest that 68Ga-NODA-aminoglucose
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Analysis of Cytokines and ATP in Plucked Hair Follicles Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2021-01-16 E. V. Mikhalchik, O. V. Morozova, T. V. Tsimbalenko, Z. F. Kharaeva, N. G. Balabushevich, V. A. Lipatova, A. G. Gadzhigoroeva
The concentrations of ATP, IL-6, and IL-10 were measured in extracts of plucked hair follicles from healthy volunteers (normal values) and patients with androgenetic alopecia and then, ATP, IL-6, and IL-10 content was calculated for each follicle. The resulting values were directly proportional to hair follicle length, except for IL-6. The concentration of extracted ATP correlated with lactate dehydrogenase
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Features of the Copy Number Variation of Certain Genes in Tumor Cells in Patients with Serous Ovarian Adenocarcinoma Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2021-01-16 D. S. Kutilin, M. R. Tsandekova, N. V. Porkhanova
We analyzed the peculiarities of the copy number variation of genes that regulate apoptosis, DNA repair, cell proliferation, metabolism, and estrogen reception in tumor and normal cells of high-grade and low-grade serous adenocarcinoma of the ovaries. Using real-time qPCR method, the relative copy number of 34 genes (BAX, BCL2, TP53, MDM2, CASP9, CASP3, CASP7, CASP8, PRKCI, SOX2, OCT4, PIK3, PTEN,
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Spatial Organization of the Transport of Interstitial Fluid and Lymph in Rat Liver (Scanning Electron Microscopy of Injection Replicas) Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2021-01-16 L. G. Patarshvili, K. B. Tsomaia, I. S. Bebiashvili, D. J. Kordzaia, S. A. Gusev
Using the method of scanning electron microscopy of injection replicas, we studied the movement of a new injection mass between the blood microcirculation system, interstitial space, lymphatic system, and bile transport system in rat liver under normal conditions and 3 days after the occlusion of the common bile duct. The casts of the perisinusoidal spaces of Disse’s after injection of the injection
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Learning against the Background of DNA Methyltransferase Inhibition Leads to the Formation of Memory That Is Resistant to Reactivation and Impairment Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2021-01-16 S. V. Solntseva, P. V. Nikitin, S. A. Kozyrev, V. P. Nikitin
The involvement of DNA methylation in the mechanisms of formation of conditioned food aversion memory was studied on Helix lucorum snails. The dynamics of aversion formation in snails injected with DNA methyltransferase inhibitor RG108 did not differ from that in control snails. The memory was retained for more than one month after training following RG108 injection and the duration of memory persistence
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Studies of Molecular Mechanisms Underlying Cardioprotective Action of the ALM-802 Compound Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2021-01-16 L. M. Kozhevnikova, V. V. Barchukov, N. P. Semenova, M. B. Vititnova, S. A. Kryzhanovskii
The mechanisms underlying cardioprotective activity of compound ALM-802 were studied in experiments on rats with chronic post-infarction heart failure. Real-time PCR showed that compound ALM-802 (daily intraperitoneal injections in a dose of 2 mg/kg for 28 days starting from day 91 after myocardial infarction modeling) restored the expression of genes encoding β1- (p=0.00001) and β2-adrenoreceptors
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Metasequencing of V3-V4 Variable Regions of 16S rRNA Gene in Opportunistic Microbiota and Gut Biocenosis in Obese Adolescents Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2021-01-16 E. V. Grigorova, N. L. Belkova, U. M. Nemchenko, E. S. Klimenko, A. V. Pogodina, A. I. Romanitsa, E. A. Novikova, L. V. Rychkova
Opportunistic microorganisms in the gut biocenosis were studied in adolescents with normal body weight and obesity (patients consulted at the Clinical Department of Research Center of Family Health and Human Reproduction Problems). The biological material was studied by standard bacteriological methods, representatives of Enterobacteriaceae family were also characterized using metagenomic sequencing
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The Role of ESR1 Gene Polymorphic Markers in the Development of Breast Cancer and Resistance to Tamoxifen Therapy Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2021-01-16 S. S. Lukina, A. M. Burdennyy, T. M. Zavarykina, D. A. Riabchikov, T. P. Kazubskaya, M. P. Kruglova, V. I. Loginov
We studied the effect of functionally significant polymorphic markers of the ESR1 gene on the risk of breast cancer, tamoxifen resistance, and survival of patients with this type of cancer. The study included 239 primary breast cancer patients without distant metastases. The analysis of genotype frequency distribution for the studied ESR1 gene polymorphic markers showed the association of the rs2228480
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Clinical and Experimental Evaluation of Diagnostic Significance of Alpha-Fetoprotein and Osteopontin at the Early Stage of Hepatocellular Cancer Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2021-01-16 Z. Macek-Jilkova, S. I. Malov, K. Kurma, C. Charrat, T. Decaens, N. P. Peretolchina, P. N. Marche, I. V. Malov, N. D. Yushchuk
We evaluated the possibility of using an experimental model of hepatocellular carcinoma to study oncomarkers of primary liver cancer and compared the diagnostic efficacy of alpha-fetoprotein and osteopontin in the experiment and in clinical practice. Experimental studies were performed on a model of hepatocellular carcinoma induced by administration of diethyl nitrosamine to Fisher-344 rats. In addition
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Effect of Original Peptide Derivatives of Galantamine on Passive Avoidance in Mice Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-03 I. I. Kostadinova, N. D. Danchev, L. T. Vezenkov, D. S. Tsekova, V. V. Rozhanets
The effect of original peptide derivatives of galantamine on scopolamine-induced memory impairment in mice was assessed using the passive avoidance test over 12 days. It was found that some galantamine derivatives administered in a dose of 1/20LD50 improved the memory in experimental mice, especially on days 5-12 of the experiment.
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Human Diploid Fibroblast Cell Lines — a Model System for Studying Immunodulatory Properties of Modern Immunobiological Drugs and Viruses Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-03 O. I. Konyuschko, S. V. Ozherelkov, M. F. Vorovich, A. L. Ivanova, S. E. Sotskova, A. A. Ishmuhametov, T. N. Kozhevnikova, A. V. Sanin
The immunomodulatory properties of immunobiological drugs Glutoxim and Phosprenyl we well as vesicular stomatitis virus and inactivated tick-borne encephalitis vaccine virus were studied using human diploid fibroblast cell line from the collection of M. P. Chumakov Federal Research Center for Research and Development of Immunobiological Products. All tested preparations exhibited immunomodulatory activity
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Analysis of the Efficiency of Different Antituberculous Drugs and Approaches to Treat BCG-Induced Granulomatosis in Mice and Abundance and Localization of Mycobacterium tuberculosis in the Liver Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-03 A. M. Sinyavskaya, V. A. Shkurupy, A. V. Troitskii, A. M. Kovner
In 3 months after infection with Mycobacterium tuberculosis (MBT) from BCG vaccine, male BALB/с mice received intraperitoneal injections of isonicotinic acid hydrazide, dextrazide, or liposome-encapsulated dextrazide, or inhalation of liposome-encapsulated dextrazide 2 times a week for 6 months. In 6 months, no MBT were detected in macrophages outside granulomas in treated mice. Macrophages containing
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Postmortem Computed Tomography Angiography of Newborns Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-03 U. N. Tumanova, V. M. Lyapin, V. G. Bychenko, A. I. Shchegolev, G. T. Sukhikh
We propose an original method of postmortem computed tomography angiography of the body of a deceased newborn. The work is based on the analysis of the results of comprehensive postmortem computed tomography and pathological examination of 30 newborns, who died from congenital malformations. The key to a full-fledged postmortem radiation study using intravascular contrasting of deceased newborns and
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Assessment of Susceptibility of Outbred Albino Rats to the Formation of Depression-Like State of Learned Helplessness Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-03 S. O. Kotel’nikova, M. S. Sadovsky, V. A. Krayneva, E. A. Valdman, S. B. Seredenin
Learned helplessness (a model of depression-like state) was developed in rats by exposure to repeated inescapable electric stimulation and evaluated by the absence of attempts to escape when it could be performed. In randomly grouped outbred white rats, 37.5% animals after the above procedure meet the criterion of learned helplessness. On experimental day 14, the latent period and the number of applied
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A Study of the Participation of NMDA Glutamate Receptors in the Mechanisms of Specific Anterograde Amnesia Reversion Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-03 S. V. Solntseva, S. A. Kozyrev, V. P. Nikitin
We studied the involvement of NMDA glutamate receptors in the mechanisms of anterograde amnesia. It was found that repeated training of amnestic animals treated with D-cycloserine, a potent agonist of the glycine site of NMDA receptors, did not lead to consolidation of long-term memory, while expression of short-term memory was more pronounced in comparison with control animals that received saline
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Mycobacterium vaccae Lysate Induces Anti-Allergic Immune Response In Vitro Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-03 A. V. Strygin, P. P. Nesmiyanov, V. I. Petrov, B. E. Tolkachev, E. I. Morkovin, M. V. Gutov, A. O. Strygina
Mycobacterium vaccae is a soil saprophyte which exerts anti-allergic properties. There are data that mechanism of action of M. vaccae when used in the treatment of human and animal allergic diseases is associated with Th1-phenotype switch. Here we studied the properties of sonicated M. vaccae lysate in co-cultures of dendritic cells and CD4+T cells. M. vaccae lysate stimulated IL-10 synthesis in co-cultures
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Ultrastructural Changes of Bacteria in Static Cultures of Yersinia pseudotuberculosis under Long Storage under Conditions of Low Temperature Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-02 L. M. Somova, N. F. Timchenko, I. N. Lyapun, E. I. Drobot, E. V. Matosova, M. P. Bynina
Electron microscopy study revealed changes in the ultrastructure of bacteria of Yersinia pseudotuberculosis strains characterized by significantly reduced reproductive ability and virulence potential after long-term storage at low temperature of 4-8°C. Most bacterial cells contained dark cytosol with reduced cellular material or empty cytosol, while the cell wall was preserved. The revealed ultrastructural
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Extracellular Matrix Proteins and Transcription of Matrix-Associated Genes in Mesenchymal Stromal Cells during Modeling of the Effects of Microgravity Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-02 I. V. Zhivodernikov, A. Yu. Ratushnyy, D. K. Matveeva, L. B. Buravkova
We studied the effects of simulated microgravity (10 days) on the production of extracellular matrix proteins and expression of extracellular matrix-associated genes in human mesenchymal stem cells. A decrease in collagen production, reduced expression of TIMP-1, TIMP-3, and MMP-11 genes, and enhanced expression of tenascin and laminin subunit were revealed. The results attest to activation of proteolytic
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Effect of Albumin on Tumor Cells In Vitro Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-02 V. G. Arzumanyan, M. V. Kiselevskii, I. M. Ozhovan, O. A. Svitich
The study examined the effect of BSA on cultured cells of breast cancer, erythroleukemia, and ovarian carcinoma. BSA cytotoxic activity was examined with light microscopy and spectrophotometry in the concentration range of 6.25 to 50 mg/ml. The high concentrations of BSA disrupted the tumor cells in parallel with formation of vesicular debris. The destruction parameters were similar in diverse types
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Integrative Approach to Assessing the Complex of Correlations between Indicators of Physiological Functions Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-02 Y. N. Smolyakov, B. I. Kuznik
We present the results of application of the combined correlation matrix method in some clinical studies. A practical algorithm is proposed for constructing a correlation matrix that compactly reflects a large number of interconnections for the situations when several diagnostic methods are used in an experimental clinical or preclinical trial. Several approaches to assessing and displaying the relationships
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Estimation of Absolute Bioavailability of the Chemical Substance of the Anti-Smallpox Preparation NIOCH-14 in Mice Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-02 O. Yu. Mazurkov, L. N. Shishkina, N. I. Bormotov, M. O. Skarnovich, O. A. Serova, N. A. Mazurkova, A. A. Chernonosov, A. Ya. Tikhonov, B. A. Selivanov
We compared absolute bioavailability of the chemical substance of the anti-smallpox preparation NIOCH-14 and chemical compound ST-246 active against orthopoxviruses after oral administration to mice in doses of 10 and 50 μg/g and intravenous administration to mice in a dose of 2 μg/g body weight. The absolute bioavailability of NIOCH-14 is comparable with the absolute bioavailability of ST-246.
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Evaluation of the Association of rs4680 COMT Polymorphism and Clinical Parameters of the Tumor in Colorectal Cancer (a Pilot Study) Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-02 T. I. Ivanova, A. L. Potapov, D. V. Zemskova, V. A. Khorokhorina, P. V. Shegai, S. A. Ivanov, A. D. Kaprin
We analyzed association of rs4680 polymorphism of catechol-O-methyltransferase (COMT) gene with clinical parameters of the tumor in patients with colorectal cancer (n=100). Based on the classification of the tumor according to the TNM system, groups were formed taking into account the size and spreading of the primary tumor (T1+T2 vs Т3+Т4) and the presence of regional (N0 vs N1) and distant metastases
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Perivascular Innervation of the Pulmonary Artery in Human and Swine: A Comparative Study for the Development of an Experimental Model of Denervation Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-02 E. G. Koshevaya, L. B. Mitrofanova, N. S. Goncharova, H. I. Condori Leandro, A. D. Vakhrushev, L. E. Korobchenko, L. A. Murashova, M. M. Galagudza, A. A. Perminova, O. M. Moiseeva, E. N. Mikhaylov
For studying the possibility of using catheter denervation of the pulmonary artery for the treatment of pulmonary hypertension, large animals, such as pigs, are more suitable, because the diameter of the pulmonary artery in this case allows manipulation of the ablation catheter. The study of the perivascular adipose tissue of the trunk and bifurcation of the pulmonary artery in humans and pigs revealed
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Changes in the Biorhythms of Biochemical Parameters in Animals with Modeled Acute Desynchronosis Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-02 E. G. Batotsyrenova, S. E. Bakulev, T. G. Nevzorova, M. B. Ivanov, V. A. Kashuro, E. A. Zolotoverkhaja, T. A. Kostrova, A. V. Sharabanov
This article describes models for the study of acute desynchronosis: jetlag syndrome and acute desynchronosis under physical stress for possible pharmacological correction of these disorders. The cosinor analysis allowed assessing significance of changes in biological rhythms in 2 biological models: the jetlag-type diurnal rhythm shift model and the model with changed light mode. The revealed changes
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In Vitro Effects of Lithium Carbonate on Cell Cycle, Apoptosis, and Autophagy in Hepatocellular Carcinoma-29 Cells Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-02 Y. S. Taskaeva, N. P. Bgatova, R. S. Dossymbekova, A. O. Solovieva, S. M. Miroshnichenko, K. O. Sharipov, Z. B. Tungushbaeva
We studied the effects of lithium carbonate on the cell cycle, apoptosis, and autophagy in hepatocellular carcinoma-29 cells (HCC-29) in vitro. Flow cytofluorometry analysis revealed accumulation of G2/M-phase HCC-29 cells and increase in the number of apoptotic cells in 48 h after administration of 5 mM lithium carbonate. Induction of autophagy in HCC-29 cells was detected by transmission electron
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Evaluation of the Protective Effect of Ademetionine, Cytoflavin, and Dihydroquercetetine on Blood Enzymes Activity in Rats Treated with High Doses of Sodium Valproate Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-02 L. V. Okhremchuk, I. Zh. Seminskii, M. A. Darenskaya, L. A. Grebenkina, L. I. Kolesnikova, S. I. Kolesnikov
We evaluated the protective effect of ademetionine, cytoflavin, and dihydroquercetin on activity of serum enzymes in rats treated with high doses of sodium valproate for 28 days. Ademetionine and cytoflavin produced the greatest protective effect, the effect of dihydroquercetin was less pronounced. In rats treated with ademetionine, AST activity decreased as soon as on day 7 and remained at this level
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Inhibitors of 3-Hydroxy-3-Methylglutaryl Coenzyme a Reductase (Statins) Suppress Differentiation and Reduce LPS/IFNγ-Induced Cytokine Production in Human Monocyte/Macrophage Culture Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-02 N. Yu. Ruleva, N. V. Radyukhina, E. S. Zubkova, A. Yu. Filatova, T. I. Aref’eva
We examined the effects of 72-h exposure to atorvastatin and rosuvastatin in concentrations of 2-10 nM on the cytokine expression in LPS/IFNγ-activated monocyte/macrophages derived from peripheral blood monocytes of healthy donors by culturing in the presence of GM-CSF. Pretreatment with statins was found to inhibit cytokine production in monocytes/macrophages after activation, while the level of cytokine
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Effect of Phosphatidylcholine Nanosomes on Phospholipid Composition of the Plasma Membranes in Liver Cells and Blood Serum in Experimental Atherosclerosis Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-02 G. F. Leskova, A. P. Kaplun, D. A. Bezrukov, A. I. Lvovsky
Alimentary atherosclerosis is associated with a significant decrease in the content of phosphatidylcholine, the phospholipid that provides antioxidant protection, in the plasma membrane of liver cells, while the level of phosphatidic acid that initiates generation of superoxides, on the contrary, increases. The level of membrane phosphatidylserine, a target of the scavenger receptors, which initiates
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Effect of Bone Marrow Autotransplantation on the Numerical Population of Tryptase-Positive Mast Cells, Heparin Sulfation Degree, and Histamine Content Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-02 O. V. Vorobyova, L. A. Lyubovtseva, V. O. Romanov, L. P. Romanova
We studied the effect of bone marrow autotransplantation on the morphofunctional properties and numerical population of mast cells. The experiments were performed on 4-monthold male mice. The animals received an injection of a suspension of bone marrow obtained from the femoral epiphyses of these animals into the caudal vein. In 40 min and 2 h after autotransplantation, the number of tryptase-positive
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Study of Immunotropic Properties of Water-Soluble Polysaccharides Isolated from Conium maculatum Grass Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-02 A. A. Ligacheva, E. Yu. Sherstoboev, M. G. Danilets, E. S. Trofimova, S. V. Krivoshchekov, A. M. Gur’ev, T. V. Bulgakov, N. V. Kudashkina, A. G. Miroshnichenko, M. V. Belousov
Addition of water-soluble polysaccharides isolated from Conium maculatum L. to the mouse peritoneal macrophage culture induces classical activation of antigen-presenting cells due to an increase in NO synthase activity and a decrease in arginase expression.
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Regulation of Vascular Smooth Muscle Contractions in the Model of Metabolic Syndrome Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-02 Yu. G. Birulina, V. V. Ivanov, E. E. Buyko, E. P. Efremkina, L. V. Smagliy, I. V. Kovalev, A. V. Nosarev, S. V. Gusakova
Reduced glucose tolerance, hyperglycemia, and imbalance in lipid levels were found in rats with metabolic syndrome induced by a high-fat, high-carbohydrate diet. The contractile responses of intact and endothelium-denuded aortic smooth muscle segments from rats with metabolic syndrome to application of acetylcholine, phenylephrine, sodium nitroprusside, and forskolin were studied by mechanographic
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Correlation of Radiation Response of Cervical Cancer Stem Cells with Their Initial Number before Treatment and Molecular Genetic Features of Papillomavirus Infection Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-02 I. A. Zamulaeva, E. I. Selivanova, V. I. Kiseleva, O. N. Matchuk, L. I. Krikunova, L. S. Mkrtchyan, A. D. Kaprin
The proportion of CD44+CD24low cancer stem cells (CSC) was determined in cervical scrapings of 41 patients with squamous cell carcinoma of the uterine cervix before treatment and after irradiation in a total focal dose of 10 Gy. The relationship of quantitative changes in the CSC population with such parameters of papillomavirus infection as genotype, viral load, and physical status of HPV DNA (the
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Peptide ACTH 4-7 -PGP: Effects on Various Types of Pain and Pain-Induced Behavior in Rats after Systemic and Central Administration Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-02 L. A. Severyanova, A. A. Kryukov, D. V. Plotnikov, M. E. Dolgintsev
The effects of peptide ACTH4-7-PGP (Semax) were studied in 12 min after its intraperitoneal (in doses of 5, 15, 50, 150, and 450 μg/kg) or intracerebroventricular (in doses of 16, 40, and 400 pg) administration to rats with different types of pain and pain-induced behavior. It was found that the peptide increased pain sensitivity and induced avoidance behavior during thermal stimulation (“hot plate”
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Possible Mechanisms of Axonal Transport Disturbances in Mouse Spinal Motoneurons Induced by Hypogravity Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-12-02 M. S. Kuznetsov, V. V. Valiullin, A. N. Lisyukov, E. S. Koshpaeva, V. R. Saitov, O. S. Razvina, M. Hayatsu, T. Ushiki, R. R. Islamov
The data obtained by transcriptome analysis of lumbar spinal cord segments, sciatic nerve, and the respiratory diaphragm of the mice performed after a space flight on board Bion-M1 biosatellite were processed by bioinformatic methods aimed at elucidation of the regularities in hypogravity-induced transcriptome changes in various compartments of motor neurons. The study revealed abnormalities of axonal
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Abnormal Vasculature Development in Zebrafish Embryos with Reduced Expression of Pantothenate Kinase 2 Gene Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-11-25 D. Khatri, L. Mignani, D. Zizioli, M. Ritelli, E. Monti, D. Finazzi
Mutations in pank2 gene encoding pantothenate kinase 2 determine a pantothenate kinase-associated neurodegeneration, a rare disorder characterized by iron deposition in the globus pallidus. To extend our previous work, we performed microinjections of a new pank2-specific morpholino to zebrafish embryos and thoroughly analyzed vasculature development. Vessels development was severely perturbed in the
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Thymalin: Activation of Differentiation of Human Hematopoietic Stem Cells Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-11-25 V. Kh. Khavinson, N. S. Linkova, I. M. Kvetnoy, V. O. Polyakova, A. O. Drobintseva, T. V. Kvetnaia, O. M. Ivko
Thymalin is a polypeptide complex isolated from the thymus and regulating the functions of the immune system. Thymalin is effective in therapy of acute respiratory syndrome, chronic obstructive bronchitis, and other immunopathology. Thymalin increases functional activity of T lymphocytes, but the targeted molecular mechanism of its biological activity requires further study. We studied the influence
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Microvesicles Produced by Natural Killer Cells Regulate the Formation of Blood Vessels Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-11-25 K. L. Markova, A. R. Kozyreva, D. I. Sokolov, S. A. Selkov
We studied the effect of microvesicles derived from cells of the NK-92 cell line on the formation of tube-like structures by endothelial cells of the ЕА.Hy926 cell line. Microvesicles were isolated by differential centrifugation and their size was controlled by granulometric analysis using dynamic light scattering method. The effect of microvesicles produced by NK cells on angiogenesis was evaluated
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Apoptotic Bodies of Cardiomyocytes and Fibroblasts — Regulators of Directed Differentiation of Heart Stem Cells Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-11-25 A. I. Tyukavin, G. B. Belostotskaya, Е. А. Zakharov, D. Yu. Ivkin, S. V. Rad’ko, N. A. Knyazev, V. V. Klimenko, A. A. Bogdanov, S. V. Suchkov
We studied the effects of apoptotic bodies of cardiomyocytes (ApBc) and fibroblasts (ApBf) on myocardial regeneration and contractility in rats and the dynamics of RNA concentrations in cardiomyocytes and fibroblasts at different stages of apoptosis. ApBc increase the contractility of rat myocardium, while ApBf reduce it. ApBc stimulate the development of clones of cardiomyocyte precursors in the myocardium
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Effect of Liposome-Encapsulated Zoledronic Acid on Microenvironment of Hepatocellular Carcinoma May Depend on the Ratio Between M1 and M2 Polarized Macrophages Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-11-25 S. Wu, X. Lin, X. Cui
We studied the effect of zoledronic acid encapsulated into liposomes (L-ZOL) on tumorassociated macrophages in the stroma of hepatocellular carcinoma xenograft. Liposomes were prepared from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, 1,2-dipalmitoyl-snglycero-3-phospho-sn-1-glycerol, and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[carboxy(polyethylene glycol)-2000] using thin film method and
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Effect of Tussilago farfara L. Polysaccharides on the Content of Hematopoietic Stem Cells (CD117 + 34 + ) in the Bone Marrow of Mice Treated with Cyclophosphamide Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-11-24 E. A. Safonova, K. A. Lopatina, A. R. Dyagel, T. G. Razina, S. G. Krylova, A. M. Gur’ev, M. V. Belousov, E. P. Zueva
Myelotoxicity is a serious side effect of anticancer drugs. The search for drugs that can reduce the hematological complications of chemotherapy through modulation of hematopoietic stem cells is an urgent task of oncopharmacology. In the present study we showed that administration of Tussilago farfara L. polysaccharides to C57BL/6 mice treated with cyclophosphamide can increase the number of hematopoietic
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AKT/mTOR Signal Cascade and Expression of PD-1, PD-L1, and PD-L2 in Gastric Cancer Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-11-24 L. V. Spirina, A. V. Avgustinovich, S. G. Afanas’ev, I. V. Kondakova, M. Yu. Volkov, A. Yu. Dobrodeev, A. I. Boronkina
The peculiarities of gastric cancer development associated with the expression levels of components of the AKT/mTOR signaling cascade and PD, PD-L1, PD-L2 have not yet been identified. We revealed the fundamental changes in the expression AKT/mTOR and PD receptors and their ligands associated with dissemination of gastric cancer. An increase in the mRNA level of all components of this cascade was demonstrated
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Expression of Epithelial Cell Adhesion Molecule (EpCAM) in Tumor Spheroids of Human Colorectal Adenocarcinoma Cells Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-11-24 A. M. Gisina, Ya. S. Kim, A. N. Gabashvili, A. V. Tsvetkova, I. V. Vakhrushev, K. N. Yarygin, A. Yu. Lupatov
We studied the formation of spheroids by Caco-2, SW480, and HCT116 human colorectal adenocarcinoma cell lines under low-adhesion culturing conditions. Of these three cell lines, only HCT116 formed stable tumor spheroids. Flow cytometry analysis of 19 surface markers in monolayer HCT116 culture and spheroids formed by these cells revealed considerable similarity of the expression profiles in these two
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Assessment of the Chemosensitivity of Uveal Melanoma Cells Ex Vivo Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-11-24 S. V. Saakyan, А. Yu. Tsygankov, N. I. Moiseeva, А. F. Karamysheva, D. D. Garri
The study was designed to create a primary cell culture of uveal melanoma and to evaluate its resistance to chemotherapy. Of the obtained 20 samples of uveal melanoma, the primary cultures with proliferation sufficient for MTT test were derived in only 7 cases. However, even these cultures were unable to survive more than 4 passages; the cells accumulated melanin and underwent apoptosis. Retinol palmitate
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Preparation of Adhesion Culture of Neural Stem/Progenitor Cells of the Olfactory Mucosa for the Treatment of Spinal Cord Injuries Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-11-24 O. V. Stepanova, A. D. Voronova, G. A. Fursa, E. K. Karsuntseva, M. P. Valikhov, A. V. Chadin, D. A. Vishnevskii, I. V. Reshetov, V. P. Chekhonin
In this work, an optimal protocol was developed for obtaining adhesion culture of neural stem/progenitor cells (NSPC) of rat olfactory mucosa. During the development of the protocol, the conditions for cell culturing on adhesion substrates fibronectin and laminin in DMEM/F-12 and neurobasal media with the same culture additives were compared. Cell proliferation was maximum during culturing on both
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Morphological Analysis of the Respiratory Tract of Rats after Parenteral Administration of Silicon Dioxide Nanoparticles Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-11-24 G. Yu. Yukina, I. V. Polovnikov, E. G. Sukhorukova, S. G. Zhuravskii, M. M. Galagudza
Morphological analysis of the respiratory tract of Wistar rats was performed after a single parenteral administration of 12-nm silicon dioxide nanoparticles (1 ml, 2 mg/ml, intravenously) was performed. On day 21 and in 2, 4, and 6 months after the administration of nanoparticles, the development of macrophage infiltration in the interstitium of the respiratory tract was demonstrated by histological
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Postmortem MRI Evaluation of Maceration Degree of Deceased Fetus Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-11-24 U. N. Tumanova, V. M. Lyapin, V. G. Bychenko, A. I. Shchegolev, G. T. Sukhikh
We studied the possibilities of postmortem MRI for assessing the degree of maceration and determining the duration of intrauterine fetal death. Postmortem radiological and pathoanatomic study of the bodies of 38 stillbirths who died antenatally (main group, n=31) and intranatally (control group, n=5), who were born at gestational periods of 22-40 weeks was performed. Before the autopsy, MRI was performed
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Experimental Study of the Effect of Biological Matrixes with Stabilized and Non-Stabilized Platelets on Reparative Process in the Wound Equivalent to Deep Burn Bull. Exp. Biol. Med. (IF 0.775) Pub Date : 2020-11-24 N. V. Borovkova, M. S. Makarov, I. N. Ponomarev, Yu. V. Andreev, M. V. Storozheva, A. A. Budaev