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Environmental Regulation of PndbA600, an Auto-Inducible Promoter for Two-Stage Industrial Biotechnology in Cyanobacteria Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-09 Mary Ann Madsen; Graham Hamilton; Pawel Herzyk; Anna Amtmann
Cyanobacteria are photosynthetic prokaryotes being developed as sustainable platforms that use renewable resources (light, water, and air) for diverse applications in energy, food, environment, and medicine. Despite the attractive promise that cyanobacteria offer to industrial biotechnology, slow growth rates pose a major challenge in processes which typically require large amounts of biomass and are
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The Role of Microenvironmental Cues and Mechanical Loading Milieus in Breast Cancer Cell Progression and Metastasis Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-22 Brandon D. Riehl; Eunju Kim; Tasneem Bouzid; Jung Yul Lim
Cancer can disrupt the microenvironments and mechanical homeostatic actions in multiple scales from large tissue modification to altered cellular signaling pathway in mechanotransduction. In this review, we highlight recent progresses in breast cancer cell mechanobiology focusing on cell-microenvironment interaction and mechanical loading regulation of cells. First, the effects of microenvironmental
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Pulmonary Surfactant and Drug Delivery: An Interface-Assisted Carrier to Deliver Surfactant Protein SP-D Into the Airways Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-21 Cristina García-Mouton; Alberto Hidalgo; Raquel Arroyo; Mercedes Echaide; Antonio Cruz; Jesús Pérez-Gil
This work is focused on the potential use of pulmonary surfactant to deliver full-length recombinant human surfactant protein SP-D (rhSP-D) using the respiratory air-liquid interface as a shuttle. Surfactant protein D (SP-D) is a collectin protein present in the pulmonary surfactant (PS) system, involved in innate immune defense and surfactant homeostasis. It has been recently suggested as a potential
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Rapid Fabrication of Cell-Laden Microfibers for Construction of Aligned Biomimetic Tissue Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-18 Bingchuan Lu; Mingfeng Li; Yongcong Fang; Zibo Liu; Ting Zhang; Zhuo Xiong
Bottom-up engineering of tissue constructs is being rapidly developed and broadly applied in biomanufacturing. As one type of building block, cell-laden microfibers are promising for reconstruction of oriented structures and functions of linear tissues, such as skeletal muscles, myocardia, and spinal cord tissues. Herein, we propose wet-spinning method with agitating collection, wherein alginate-based
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Topographic Guidance in Melt-Electrowritten Tubular Scaffolds Enhances Engineered Kidney Tubule Performance Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-16 Anne Metje van Genderen; Katja Jansen; Marleen Kristen; Joost van Duijn; Yang Li; Carl C. L. Schuurmans; Jos Malda; Tina Vermonden; Jitske Jansen; Rosalinde Masereeuw; Miguel Castilho
Introduction: To date, tubular tissue engineering relies on large, non-porous tubular scaffolds (Ø > 2 mm) for mechanical self-support, or smaller (Ø 150–500 μm) tubes within bulk hydrogels for studying renal transport phenomena. To advance the engineering of kidney tubules for future implantation, constructs should be both self-supportive and yet small-sized and highly porous. Here, we hypothesize
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Bringing MicroRNAs to Light: Methods for MicroRNA Quantification and Visualization in Live Cells Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-15 Tarana Siddika; Ilka U. Heinemann
MiRNAs are small non-coding RNAs that interact with their target mRNAs for posttranscriptional gene regulation. Finely controlled miRNA biogenesis, target recognition and degradation indicate that maintaining miRNA homeostasis is essential for regulating cell proliferation, growth, differentiation and apoptosis. Increasingly, miRNAs have been recognized as a potential biomarker for disease diagnosis
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Modulating the Mechanical Activation of TRPV4 at the Cell-Substrate Interface Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-15 Setareh Sianati; Lioba Schroeter; Jessica Richardson; Andy Tay; Shireen R. Lamandé; Kate Poole
Ion channels activated by mechanical inputs are important force sensing molecules in a wide array of mammalian cells and tissues. The transient receptor potential channel, TRPV4, is a polymodal, nonselective cation channel that can be activated by mechanical inputs but only if stimuli are applied directly at the interface between cells and their substrate, making this molecule a context-dependent force
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Developing a Tooth in situ Organ Culture Model for Dental and Periodontal Regeneration Research Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-10 Reem El-Gendy; Sarah Junaid; Stephen K. L. Lam; Karen M. Elson; Joanne L. Tipper; Richard M. Hall; Eileen Ingham; Jennifer Kirkham
In this study we have realized the need for an organ culture tooth in situ model to simulate the tooth structure especially the tooth attachment apparatus. The importance of such a model is to open avenues for investigating regeneration of the complex tooth and tooth attachment tissues and to reduce the need for experimental animals in investigating dental materials and treatments in the future. The
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Nanomaterial Nitric Oxide Delivery in Traumatic Orthopedic Regenerative Medicine Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-10 Albert Thomas Anastasio; Ariana Paniagua; Carrie Diamond; Harrison R. Ferlauto; Joseph S. Fernandez-Moure
Achieving bone fracture union after trauma represents a major challenge for the orthopedic surgeon. Fracture non-healing has a multifactorial etiology and there are many risk factors for non-fusion. Environmental factors such as wound contamination, infection, and open fractures can contribute to non-healing, as can patient specific factors such as poor vascular status and improper immunologic response
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Functional Gradient Metallic Biomaterials: Techniques, Current Scenery, and Future Prospects in the Biomedical Field Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-10 Hongyuan Shi; Peng Zhou; Jie Li; Chaozong Liu; Liqiang Wang
Functional gradient materials (FGMs), as a modern group of materials, can provide multiple functions and are able to well mimic the hierarchical and gradient structure of natural systems. Because biomedical implants usually substitute the bone tissues and bone is an organic, natural FGM material, it seems quite reasonable to use the FGM concept in these applications. These FGMs have numerous advantages
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Deciphering the Potential of Rhizobium pusense MB-17a, a Plant Growth-Promoting Root Endophyte, and Functional Annotation of the Genes Involved in the Metabolic Pathway Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-11-30 Twinkle Chaudhary; Rajesh Gera; Pratyoosh Shukla
Plant growth-promoting rhizobacteria (PGPR) are root endophytic bacteria used for growth promotion, and they have broader applications in enhancing specific crop yield as a whole. In the present study, we have explored the potential of Rhizobium pusense MB-17a as an endophytic bacterium isolated from the roots of the mung bean (Vigna radiata) plant. Furthermore, this bacterium was sequenced and assembled
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Characterization of the Biodistribution of a Silica Vesicle Nanovaccine Carrying a Rhipicephalus (Boophilus) microplus Protective Antigen With in vivo Live Animal Imaging Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-11-30 Karishma T. Mody; Bing Zhang; Xun Li; Nicholas L. Fletcher; Dewan T. Akhter; Sandy Jarrett; Jun Zhang; Chengzhong Yu; Kristofer J. Thurecht; Timothy J. Mahony; Neena Mitter
Development of veterinary subunit vaccines comes with a spectrum of challenges, such as the choice of adjuvant, antigen delivery vehicle, and optimization of dosing strategy. Over the years, our laboratory has largely focused on investigating silica vesicles (SVs) for developing effective veterinary vaccines for multiple targets. Rhipicephalus microplus (cattle tick) are known to have a high impact
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Performance of AnMBR in Treatment of Post-consumer Food Waste: Effect of Hydraulic Retention Time and Organic Loading Rate on Biogas Production and Membrane Fouling Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-11-23 Javkhlan Ariunbaatar; Robert Bair; Onur Ozcan; Harish Ravishankar; Giovanni Esposito; Piet N. L. Lens; Daniel H. Yeh
Anaerobic digestion of food waste (FW) is typically limited to large reactors due to high hydraulic retention times (HRTs). Technologies such as anaerobic membrane reactors (AnMBRs) can perform anaerobic digestion at lower HRTs while maintaining high chemical oxygen demand (COD) removal efficiencies. This study evaluated the effect of HRT and organic loading rate (OLR) on the stability and performance
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Microfluidic Point-of-Care Testing: Commercial Landscape and Future Directions Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-15 Shivangi Sachdeva; Ronald W. Davis; Amit K. Saha
Point-of-care testing (POCT) allows physicians to detect and diagnose diseases at or near the patient site, faster than conventional lab-based testing. The importance of POCT is considerably amplified in the trying times of the COVID-19 pandemic. Numerous point-of-care tests and diagnostic devices are available in the market including, but not limited to, glucose monitoring, pregnancy and infertility
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Aggregation-Induced Emission of Au/Ag Alloy Nanoclusters for Fluorescence Detection of Inorganic Pyrophosphate and Pyrophosphatase Activity Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-15 Zhongli Lei; Jie Zhou; Miao Liang; Yan Xiao; Zhihong Liu
The development of sensitive and accurate detection of inorganic pyrophosphate (PPi) and pyrophosphatase activity (PPase) is important as they play vital roles in biological systems. However, it is still not satisfactory for most of the analytical methods for PPi and PPase because of their Cu2+-dependence and poor accuracy. Although the metal ion triggered aggregation-induced emission (AIE) of metal
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Hydrostatic Pressure Regulates the Volume, Aggregation and Chondrogenic Differentiation of Bone Marrow Derived Stromal Cells Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-15 Paola Aprile; Daniel J. Kelly
The limited ability of articular cartilage to self-repair has motivated the development of tissue engineering strategies that aim to harness the regenerative potential of mesenchymal stem/marrow stromal cells (MSCs). Understanding how environmental factors regulate the phenotype of MSCs will be central to unlocking their regenerative potential. The biophysical environment is known to regulate the phenotype
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Comparative Analysis of Mesenchymal Stem Cell Cultivation in Fetal Calf Serum, Human Serum, and Platelet Lysate in 2D and 3D Systems Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-08 Marline Kirsch; Jessica Rach; Wiebke Handke; Axel Seltsam; Iliyana Pepelanova; Sarah Strauß; Peter Vogt; Thomas Scheper; Antonina Lavrentieva
In vitro two-dimensional (2D) and three-dimensional (3D) cultivation of mammalian cells requires supplementation with serum. Mesenchymal stem cells (MSCs) are widely used in clinical trials for bioregenerative medicine and in most cases, in vitro expansion and differentiation of these cells are required before application. Optimized expansion and differentiation protocols play a key role in the treatment
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Changes in Bacterial Community Structure and Enriched Functional Bacteria Associated With Turfgrass Monoculture Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-07 Chang-Wook Jeon; Da-Ran Kim; Eun-Ji Bae; Youn-Sig Kwak
There is increasing attention being paid to utilizing microbial communities to improve plant health while reducing management inputs. Thus, the objectives of this research were to assess changes in the rhizosphere bacterial community structure associated with long-term turfgrass monoculture and to demonstrate the feasibility of using functional bacteria as beneficial biocontrol agents. Large patch
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Axial Micromotion Locking Plate Construct Can Promote Faster and Stronger Bone Healing in an Ovine Osteotomy Model Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-04 Zhihua Han; Jianhong Wu; Guoying Deng; Chun Bi; Jiandong Wang; Qiugen Wang
Fixing bone fractures with controlled axial interfragmentary micromotion improves bone healing; however, the optimal type of implant construct for this purpose is still lacking. The present study describes a novel axial micromotion locking plate (AMLP) construct that allows axial interfragmentary micromotion of 0.3 or 0.6 mm. We investigated whether the AMLP constructs enhance bone healing compared
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Exosomes of Human Umbilical Cord MSCs Protect Against Hypoxia/Reoxygenation-Induced Pyroptosis of Cardiomyocytes via the miRNA-100-5p/FOXO3/NLRP3 Pathway Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-02 Chenyu Liang; Yang Liu; Huifeng Xu; Junling Huang; Yi Shen; Faxiu Chen; Ming Luo
Background Acute myocardial infarction (AMI) is one of the leading causes of morbidity and death worldwide. Studies have indicated that microRNAs in mesenchymal stem cell (MSC)-derived exosomes are crucial for treating various diseases. Methods Human umbilical cord MSC (hucMSC)-derived exosomes (hucMSC-exo) were isolated and used to treat cardiomyocytes that underwent hypoxia/reoxygenation (H/R) injury
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Isolation and Identification of Organics-Degrading Bacteria From Gas-to-Liquid Process Water Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-11-30 Riham Surkatti; Zulfa A. Al Disi; Muftah H. El-Naas; Nabil Zouari; Mark C. M. Van Loosdrecht; Udeogu Onwusogh
The gas-to-liquid (GTL) process generates considerable amounts of wastewater that are highly acidic and characterized by its high chemical oxygen demand (COD) content, due to the presence of several organic pollutants, such as alcohols, ketones, aldehydes, and fatty acids. The presence of these organics in the process water may lead to adverse effect on the environment and aquatic life. Thus, it is
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ROS Induce β-Carotene Biosynthesis Caused by Changes of Photosynthesis Efficiency and Energy Metabolism in Dunaliella salina Under Stress Conditions Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-11-25 Yimei Xi; Fantao Kong; Zhanyou Chi
The unicellular alga Dunaliella salina is regarded as a promising cell factory for the commercial production of β-carotene due to its high yield of carotenoids. However, the underlying mechanism of β-carotene accumulation is still unclear. In this study, the regulatory mechanism of β-carotene accumulation in D. salina under stress conditions was investigated. Our results indicated that there is a significant
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A Single Amino Acid Change to Taq DNA Polymerase Enables Faster PCR, Reverse Transcription and Strand-Displacement Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-22 Wayne M. Barnes; Zhian Zhang; Milko B. Kermekchiev
A change of an aspartic acid to asparagine of Taq (Thermus aquaticus) DNA polymerase is a gain of function mutation that supports faster PCR: the extension times for PCR amplification can be 2–3 times shorter. Surprising results from negative controls led to the discovery of strand-displacement ability and reverse transcriptase activity of Taq D732N DNA polymerase. We demonstrate that the mutant enzyme
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Optimizing the Intracellular Delivery of Therapeutic Anti-inflammatory TNF-α siRNA to Activated Macrophages Using Lipidoid-Polymer Hybrid Nanoparticles Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-15 Abhijeet Lokras; Aneesh Thakur; Abishek Wadhwa; Kaushik Thanki; Henrik Franzyk; Camilla Foged
RNA interference (RNAi) has an unprecedented potential as a therapeutic strategy for reversibly silencing the expression of any gene. Therapeutic delivery of the RNAi mediator, i.e., small interfering RNA (siRNA), can be used to address diseases characterized by gene overexpression, for example inflammatory conditions like chronic obstructive pulmonary disease (COPD). Macrophages play a key role in
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Development of Small-Diameter Elastin-Silk Fibroin Vascular Grafts Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-15 Takashi Tanaka; Yasuyuki Abe; Chieh-Jen Cheng; Ryo Tanaka; Akira Naito; Tetsuo Asakura
Globally, increasing mortality from cardiovascular disease has become a problem in recent years. Vascular replacement has been used as a treatment for these diseases, but with blood vessels <6 mm in diameter, existing vascular grafts made of synthetic polymers can be occluded by thrombus formation or intimal hyperplasia. Therefore, the development of new artificial vascular grafts is desirable. In
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Different Machine Learning and Deep Learning Methods for the Classification of Colorectal Cancer Lymph Node Metastasis Images Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-14 Jin Li; Peng Wang; Yang Zhou; Hong Liang; Kuan Luan
The classification of colorectal cancer (CRC) lymph node metastasis (LNM) is a vital clinical issue related to recurrence and design of treatment plans. However, it remains unclear which method is effective in automatically classifying CRC LNM. Hence, this study compared the performance of existing classification methods, i.e., machine learning, deep learning, and deep transfer learning, to identify
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A Review: Electrode and Packaging Materials for Neurophysiology Recording Implants Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-10 Weiyang Yang; Yan Gong; Wen Li
To date, a wide variety of neural tissue implants have been developed for neurophysiology recording from living tissues. An ideal neural implant should minimize the damage to the tissue and perform reliably and accurately for long periods of time. Therefore, the materials utilized to fabricate the neural recording implants become a critical factor. The materials of these devices could be classified
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A Novel Macro-Micro Approach for Swimming Analysis in Main Swimming Techniques Using IMU Sensors Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-10 Mahdi Hamidi Rad; Vincent Gremeaux; Farzin Dadashi; Kamiar Aminian
Inertial measurement units (IMU) are proven as efficient tools for swimming analysis by overcoming the limits of video-based systems application in aquatic environments. However, coaches still believe in the lack of a reliable and easy-to-use analysis system for swimming. To provide a broad view of swimmers' performance, this paper describes a new macro-micro analysis approach, comprehensive enough
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Estimating Movement Smoothness From Inertial Measurement Units Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-09 Alejandro Melendez-Calderon; Camila Shirota; Sivakumar Balasubramanian
Inertial measurement units (IMUs) are increasingly used to estimate movement quality and quantity to the infer the nature of motor behavior. The current literature contains several attempts to estimate movement smoothness using data from IMUs, many of which assume that the translational and rotational kinematics measured by IMUs can be directly used with the smoothness measures spectral arc length
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Control Theory and Cancer Chemotherapy: How They Interact Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-09 Paola Lecca
Control theory arises in most modern real-life applications, not least in biological and medical applications. In particular, in biological and medical contexts, the role of control theory began to take shape in the early 1980s when the first works appeared on the application of control theory in models of pharmacokinetics and pharmacodynamics for antitumor therapies. Forty years after those first
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Perinatal Cells: A Promising COVID-19 Therapy? Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-08 Andrea Papait; Anna Cargnoni; Michal Sheleg; Antonietta R. Silini; Gilad Kunis; Racheli Ofir; Ornella Parolini
The COVID-19 pandemic has become a priority in the health systems of all nations worldwide. In fact, there are currently no specific drugs or preventive treatments such as vaccines. The numerous therapies available today aim to counteract the symptoms caused by the viral infection that in some subjects can evolve causing acute respiratory distress syndromes (ARDS) with consequent admission to intensive
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Copper-Containing Alloy as Immunoregulatory Material in Bone Regeneration via Mitochondrial Oxidative Stress Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-08 Daorong Xu; Jikun Qian; Xin Guan; Ling Ren; Kaifan Yang; Xuan Huang; Shuyuan Zhang; Yu Chai; Xiaohu Wu; Hangtian Wu; Xianrong Zhang; Ke Yang; Bin Yu
In the mammalian skeletal system, osteogenesis and angiogenesis are closely linked by type H vessels during bone regeneration and repair. Our previous studies confirmed the promotion of these processes by copper-containing metal (CCM) in vitro and in vivo. However, whether and how the coupling of angiogenesis and osteogenesis participates in the promotion of bone regeneration by CCM in vivo is unknown
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PCR & Go: A Pre-installed Expression Chassis for Facile Integration of Multi-Gene Biosynthetic Pathways Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-07 Mingming Qi; Bei Zhang; Lihong Jiang; Saijuan Xu; Chang Dong; Yi-Ling Du; Zhan Zhou; Lei Huang; Zhinan Xu; Jiazhang Lian
The introduction of multi-gene metabolic pathways is generally the first step for the construction of microbial cell factories and plays an essential role in metabolic engineering and synthetic biology. Here, we developed a “PCR & Go” system for facile integration and assembly of multi-gene pathways into the chromosome of Saccharomyces cerevisiae. The core component of the “PCR & Go” system was an
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Facile Synthesis of Porous ZnO Nanoparticles Efficient for Photocatalytic Degradation of Biomass-Derived Bisphenol A Under Simulated Sunlight Irradiation Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-02 Yujie Wang; Kang Hu; Zhiyu Yang; Chenlu Ye; Xin Li; Kai Yan
Bisphenol A (BPA) produced from biomass is a typical endocrine disrupting compound that is carcinogenic and genotoxic and can be accumulated in water due to its extensive use and difficult degradation. In this study, the porous ZnO photocatalyst with core-shell structure and large surface area was successfully developed for the efficient photocatalytic degradation of BPA. The various effects of calcination
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In vivo and Post-synthesis Strategies to Enhance the Properties of PHB-Based Materials: A Review Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-11-30 Rosa Turco; Gabriella Santagata; Iolanda Corrado; Cinzia Pezzella; Martino Di Serio
The transition toward “green” alternatives to petroleum-based plastics is driven by the need for “drop-in” replacement materials able to combine characteristics of existing plastics with biodegradability and renewability features. Promising alternatives are the polyhydroxyalkanoates (PHAs), microbial biodegradable polyesters produced by a wide range of microorganisms as carbon, energy, and redox storage
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Metabolic Glycoengineering Enables the Ultrastructural Visualization of Sialic Acids in the Glycocalyx of the Alveolar Epithelial Cell Line hAELVi Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-11-23 Raphael Brandt; Sara Timm; Jacob L. Gorenflos López; Jubilant Kwame Abledu; Wolfgang M. Kuebler; Christian P. R. Hackenberger; Matthias Ochs; Elena Lopez-Rodriguez
The glycocalyx—a plethora of sugars forming a dense layer that covers the cell membrane—is commonly found on the epithelial surface of lumen forming tissue. New glycocalyx specific properties have been defined for various organs in the last decade. However, in the lung alveolar epithelium, its structure and functions remain almost completely unexplored. This is partly due to the lack of physiologically
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Control Modification of Grasp Force Covaries Agency and Performance on Rigid and Compliant Surfaces Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-16 Raviraj Nataraj; Sean Sanford
This study investigated how modifications in the display of a computer trace under user control of grasp forces can co-modulate agency (perception of control) and performance of grasp on rigid and compliant surfaces. We observed positive correlation (p < 0.01) between implicit agency, measured from time-interval estimation for intentional binding, and grasp performance, measured by force-tracking error
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High-Throughput Plant Phenotyping Platform (HT3P) as a Novel Tool for Estimating Agronomic Traits From the Lab to the Field Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-15 Daoliang Li; Chaoqun Quan; Zhaoyang Song; Xiang Li; Guanghui Yu; Cheng Li; Akhter Muhammad
Food scarcity, population growth, and global climate change have propelled crop yield growth driven by high-throughput phenotyping into the era of big data. However, access to large-scale phenotypic data has now become a critical barrier that phenomics urgently must overcome. Fortunately, the high-throughput plant phenotyping platform (HT3P), employing advanced sensors and data collection systems,
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Polyhydroxyalkanoate Synthesis by Burkholderia glumae into a Sustainable Sugarcane Biorefinery Concept Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-15 Carolina Bilia Chimello de Paula; Fabrício Coutinho de Paula-Elias; Marcela Nogueira Rodrigues; Luciana Fontes Coelho; Nayra Morgana Lima de Oliveira; Alex Fernando de Almeida; Jonas Contiero
Polyhydroxyalkanoate (PHA) bioplastic was synthesized by Burkholderia glumae MA13 from carbon sources and industrial byproducts related to sugarcane biorefineries: sucrose, xylose, molasses, vinasse, bagasse hydrolysate, yeast extract, yeast autolysate, and inactivated dry yeast besides different inorganic nitrogen sources. Sugarcane molasses free of pre-treatment was the best carbon source, even compared
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Non-viral Gene Therapy for Osteoarthritis Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-11 Ilona Uzieliene; Ursule Kalvaityte; Eiva Bernotiene; Ali Mobasheri
Strategies for delivering nucleic acids into damaged and diseased tissues have been divided into two major areas: viral and non-viral gene therapy. In this mini-review article we discuss the application of gene therapy for the treatment of osteoarthritis (OA), one of the most common forms of arthritis. We focus primarily on non-viral gene therapy and cell therapy. We briefly discuss the advantages
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Laminin-Modified Dental Pulp Extracellular Matrix for Dental Pulp Regeneration Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-09 Jiahui Fu; Jianfeng Chen; Wenjun Li; Xiaomin Yang; Jingyan Yang; Huixin Quan; Haitao Huang; Gang Chen
Native dental pulp extracellular matrix (DPEM) has proven to be an effective biomaterial for dental pulp regeneration. However, as a significant extracellular matrix glycoprotein, partial laminins were lost during the decellularization process, which were essential for odontoblast differentiation. Thereby, this study investigated the feasibility of LN supplementation to improve the surface of DPEM
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Sub-Regional Differences of the Human Amniotic Membrane and Their Potential Impact on Tissue Regeneration Application Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-07 Adelheid Weidinger; Laura Poženel; Susanne Wolbank; Asmita Banerjee
For more than 100 years, the human amniotic membrane (hAM) has been used in multiple tissue regeneration applications. The hAM consists of cells with stem cell characteristics and a rich layer of extracellular matrix. Undoubtedly, the hAM with viable cells has remarkable properties such as the differentiation potential into all three germ layers, immuno-modulatory, and anti-fibrotic properties. At
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Use of Cell Envelope Targeting Antibiotics and Antimicrobial Agents as a Powerful Tool to Select for Lactic Acid Bacteria Strains With Improved Texturizing Ability in Milk Fermentations Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-07 Kim I. Sørensen; Inge Kjærbølling; Ana Rute Neves; Ronnie Machielsen; Eric Johansen
Many antibiotics and antimicrobial agents have the bacterial cell envelope as their primary target, interfering with functions such as synthesis of peptidoglycan, membrane stability and permeability, and attachment of surface components. The cell envelope is the outermost barrier of the bacterial cell, conferring protection against environmental stresses, and maintaining structural integrity and stability
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A New Method for Chromosomes Preparation by ATP-Competitive Inhibitor SP600125 via Enhancement of Endomitosis in Fish Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-03 Wenting Xu; Yanxiu Mo; Yu He; Yunpeng Fan; Guomin He; Wen Fu; Shujuan Chen; Jinhui Liu; Wenbin Liu; Liangyue Peng; Yamei Xiao
Previous studies have suggested that 1,9-Pyrazoloanthrone, known as SP600125, can induce cell polyploidization. However, what is the phase of cell cycle arrest caused by SP600125 and the underlying regulation is still an interesting issue to be further addressed. Research in this article shows that SP600125 can block cell cycle progression at the prometaphase of mitosis and cause endomitosis. It is
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Carbon Nanotube Modified Microelectrode Array for Neural Interface Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-11-30 Mohaddeseh Vafaiee; Raheleh Mohammadpour; Manouchehr Vossoughi; Elham Asadian; Mahyar Janahmadi; Pezhman Sasanpour
Carbon nanotubes (CNTs) coatings have been shown over the past few years as a promising material for neural interface applications. In particular, in the field of nerve implants, CNTs have fundamental advantages due to their unique mechanical and electrical properties. In this study, carbon nanotubes multi-electrode arrays (CNT-modified-Au MEAs) were fabricated based on gold multi-electrode arrays
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A Review on Modifications of Amniotic Membrane for Biomedical Applications Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-11-25 Fatemeh Dadkhah Tehrani; Arezoo Firouzeh; Iman Shabani; Azadeh Shabani
The amniotic membrane (AM) is the innermost layer of the fetal placenta, which surrounds and protects the fetus. Its unique structure, in addition to its physical and biological properties, makes it a useful substance in many applications related to regenerative medicine. The use of this fantastic substance with a century-old history has produced remarkable results in vivo, in vitro, and even in clinical
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Simulation Analysis of Impulsive Ankle Push-Off on the Walking Speed of a Planar Biped Robot Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-16 Qiaoli Ji; Zhihui Qian; Lei Ren; Luquan Ren
Ankle push-off generates more than 80% positive power at the end of the stance phase during human walking. In this paper, the influence of impulsive ankle push-off on the walking speed of a biped robot is studied by simulation. When the push-off height of the ankle joint is 13 cm based on the ground (the height of the ankle joint of the swing leg) and the ankle push-off torque increases from 17 to
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Graphene-Modified Titanium Surface Enhances Local Growth Factor Adsorption and Promotes Osteogenic Differentiation of Bone Marrow Stromal Cells Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-14 Jiayu Lu; Jiayue Sun; Derong Zou; Jinlin Song; Sheng Yang
Graphene coating exhibits excellent abilities of protein adsorption and cell adhesion, which might expand the osteogenic activity of titanium implant surface to adapt to the environment of low bone mass and poor bone quality. In this paper, we designed and explored the graphene-coated titanium sheet, through the surface modification of oxygen-containing functional groups, to optimize the adsorption
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A Carbon Fixation Enhanced Chlamydomonas reinhardtii Strain for Achieving the Double-Win Between Growth and Biofuel Production Under Non-stressed Conditions Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-11 Zhen Zhu; Huijiao Cao; Xu Li; Junfeng Rong; Xupeng Cao; Jing Tian
The stressed cultivations are widely used in microalgae R&D for the biofuel production with the repress on growth to a certain degree, which limits the overall productivity. The balance between the growth and energy storage compounds accumulation is a target needing the combination of both strain selection or construction and culture optimization. Here, an engineered strain of Chlamydomonas reinhardtii
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Bioinspired Modifications of PEEK Implants for Bone Tissue Engineering Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-10 Xinming Gu; Xiaolin Sun; Yue Sun; Jia Wang; Yiping Liu; Kaixuan Yu; Yao Wang; Yanmin Zhou
In recent years, polyetheretherketone (PEEK) has been increasingly employed as an implant material in clinical applications. Although PEEK is biocompatible, chemically stable, and radiolucent and has an elastic modulus similar to that of natural bone, it suffers from poor integration with surrounding bone tissue after implantation. To improve the bioactivity of PEEK, numerous strategies for functionalizing
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Rapid Detection of Pomelo Fruit Quality Using Near-Infrared Hyperspectral Imaging Combined With Chemometric Methods Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-07 Huazhou Chen; Hanli Qiao; Quanxi Feng; Lili Xu; Qinyong Lin; Ken Cai
Pomelo is an important agricultural product in southern China. Near-infrared hyperspectral imaging (NIRHI) technology is applied to the rapid detection of pomelo fruit quality. Advanced chemometric methods have been investigated for the optimization of the NIRHI spectral calibration model. The partial least squares (PLS) method is improved for non-linear regression by combining it with the kernel Gaussian
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Hydroxyapatite Particle Density Regulates Osteoblastic Differentiation Through β-Catenin Translocation Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-08 Otto J. Juhl; Anna-Blessing Merife; Yue Zhang; Christopher A. Lemmon; Henry J. Donahue
Substrate surface characteristics such as roughness, wettability and particle density are well-known contributors of a substrate's overall osteogenic potential. These characteristics are known to regulate cell mechanics as well as induce changes in cell stiffness, cell adhesions, and cytoskeletal structure. Pro-osteogenic particles, such as hydroxyapatite, are often incorporated into a substrate to
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Production of Long Chain Fatty Alcohols Found in Bumblebee Pheromones by Yarrowia lipolytica Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-08 Jaroslav Hambalko; Peter Gajdoš; Jean-Marc Nicaud; Rodrigo Ledesma-Amaro; Michal Tupec; Iva Pichová; Milan Čertík
Fatty alcohols (FA-OH) are aliphatic unbranched primary alcohols with a chain of four or more carbon atoms. Besides potential industrial applications, fatty alcohols have important biological functions as well. In nature, fatty alcohols are produced as a part of a mixture of pheromones in several insect species, such as moths, termites, bees, wasps, etc. In addition, FA-OHs have a potential for agricultural
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Multi-Omics Driven Metabolic Network Reconstruction and Analysis of Lignocellulosic Carbon Utilization in Rhodosporidium toruloides Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-04 Joonhoon Kim; Samuel T. Coradetti; Young-Mo Kim; Yuqian Gao; Junko Yaegashi; Jeremy D. Zucker; Nathalie Munoz; Erika M. Zink; Kristin E. Burnum-Johnson; Scott E. Baker; Blake A. Simmons; Jeffrey M. Skerker; John M. Gladden; Jon K. Magnuson
An oleaginous yeast Rhodosporidium toruloides is a promising host for converting lignocellulosic biomass to bioproducts and biofuels. In this work, we performed multi-omics analysis of lignocellulosic carbon utilization in R. toruloides and reconstructed the genome-scale metabolic network of R. toruloides. High-quality metabolic network models for model organisms and orthologous protein mapping were
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Tailoring Cellular Function: The Contribution of the Nucleus in Mechanotransduction Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-02 Fabrizio A. Pennacchio; Paulina Nastały; Alessandro Poli; Paolo Maiuri
Cells sense a variety of different mechanochemical stimuli and promptly react to such signals by reshaping their morphology and adapting their structural organization and tensional state. Cell reactions to mechanical stimuli arising from the local microenvironment, mechanotransduction, play a crucial role in many cellular functions in both physiological and pathological conditions. To decipher this
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Engineering Photosynthetic Bioprocesses for Sustainable Chemical Production: A Review Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-01 Sheida Stephens; Radhakrishnan Mahadevan; D. Grant Allen
Microbial production of chemicals using renewable feedstocks such as glucose has emerged as a green alternative to conventional chemical production processes that rely primarily on petroleum-based feedstocks. The carbon footprint of such processes can further be reduced by using engineered cells that harness solar energy to consume feedstocks traditionally considered to be wastes as their carbon sources
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Synthetic Organic Compounds From Paper Industry Wastes: Integrated Biotechnological Interventions Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-11-30 Shweta Jaiswal; Guddu Kumar Gupta; Kusum Panchal; Mandeep; Pratyoosh Shukla
Synthetic organic compounds (SOCs) are reported as xenobiotics compounds contaminating the environment from various sources including waste from the pulp and paper industries: Since the demand and production of paper is growing increasingly, the release of paper and pulp industrial waste consisting of SOCs is also increasing the SOCs’ pollution in natural reservoirs to create environmental pollution
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How Well Do Commonly Used Co-contraction Indices Approximate Lower Limb Joint Stiffness Trends During Gait for Individuals Post-stroke? Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-09 Geng Li; Mohammad S. Shourijeh; Di Ao; Carolynn Patten; Benjamin J. Fregly
Muscle co-contraction generates joint stiffness to improve stability and accuracy during limb movement but at the expense of higher energetic cost. However, quantification of joint stiffness is difficult using either experimental or computational means. In contrast, quantification of muscle co-contraction using an EMG-based Co-Contraction Index (CCI) is easier and may offer an alternative for estimating
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The Promises and Realities of Integration in Synthetic Biology: A View From Social Science Front. Bioeng. Biotech. (IF 3.644) Pub Date : 2020-12-07 Lucy Carter; Aditi Mankad
Discussions about the responsible advancement of synthetic biology science are at fever pitch. Commentators from across the globe are calling for greater integrated science investments and more inclusive governance processes in the development and implementation of these potentially disruptive technologies. We take stock of the promises and realities of science integration by sharing our experiences