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YME1L-mediated mitophagy protects renal tubular cells against cellular senescence under diabetic conditions Biol. Res. (IF 6.7) Pub Date : 2024-03-17 Yuanyuan Luo, Lingxiao Zhang, Ning Su, Lerong Liu, Tongfeng Zhao
The senescence of renal tubular epithelial cells (RTECs) is crucial in the progression of diabetic kidney disease (DKD). Accumulating evidence suggests a close association between insufficient mitophagy and RTEC senescence. Yeast mitochondrial escape 1-like 1 (YME1L), an inner mitochondrial membrane metalloprotease, maintains mitochondrial integrity. Its functions in DKD remain unclear. Here, we investigated
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Effects of latroeggtoxin-VI on dopamine and α-synuclein in PC12 cells and the implications for Parkinson’s disease Biol. Res. (IF 6.7) Pub Date : 2024-03-16 Dianmei Yu, Haiyan Wang, Yiwen Zhai, Zhixiang Lei, Minglu Sun, Si Chen, Panfeng Yin, Xianchun Wang
Parkinson’s disease (PD) is characterized by death of dopaminergic neurons leading to dopamine deficiency, excessive α-synuclein facilitating Lewy body formation, etc. Latroeggtoxin-VI (LETX-VI), a proteinaceous neurotoxin discovered from the eggs of spider L. tredecimguttatus, was previously found to promote the synthesis and release of PC12 cells, showing a great potential as a drug candidate for
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Glial-restricted progenitor cells: a cure for diseased brain? Biol. Res. (IF 6.7) Pub Date : 2024-03-12 Piotr Rogujski, Barbara Lukomska, Miroslaw Janowski, Luiza Stanaszek
The central nervous system (CNS) is home to neuronal and glial cells. Traditionally, glia was disregarded as just the structural support across the brain and spinal cord, in striking contrast to neurons, always considered critical players in CNS functioning. In modern times this outdated dogma is continuously repelled by new evidence unravelling the importance of glia in neuronal maintenance and function
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Carbapenem-resistant hypervirulent ST23 Klebsiella pneumoniae with a highly transmissible dual-carbapenemase plasmid in Chile Biol. Res. (IF 6.7) Pub Date : 2024-03-12 Matías Gálvez-Silva, Patricio Arros, Camilo Berríos-Pastén, Aura Villamil, Paula I. Rodas, Ingrid Araya, Rodrigo Iglesias, Pamela Araya, Juan C. Hormazábal, Constanza Bohle, Yahua Chen, Yunn-Hwen Gan, Francisco P. Chávez, Rosalba Lagos, Andrés E. Marcoleta
The convergence of hypervirulence and carbapenem resistance in the bacterial pathogen Klebsiella pneumoniae represents a critical global health concern. Hypervirulent K. pneumoniae (hvKp) strains, frequently from sequence type 23 (ST23) and having a K1 capsule, have been associated with severe community-acquired invasive infections. Although hvKp were initially restricted to Southeast Asia and primarily
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Endometrial mesenchymal stromal/stem cells improve regeneration of injured endometrium in mice Biol. Res. (IF 6.7) Pub Date : 2024-02-13 Tianqi Li, Rachel W.S. Chan, Raymond H.W. Li, Ernest H.Y. Ng, Songying Zhang, William S.B. Yeung
The monthly regeneration of human endometrial tissue is maintained by the presence of human endometrial mesenchymal stromal/stem cells (eMSC), a cell population co-expressing the perivascular markers CD140b and CD146. Endometrial regeneration is impaired in the presence of intrauterine adhesions, leading to infertility, recurrent pregnancy loss and placental abnormalities. Several types of somatic
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Embryo development is impaired by sperm mitochondrial-derived ROS Biol. Res. (IF 6.7) Pub Date : 2024-01-30 Yentel Mateo-Otero, Marc Llavanera, Marc Torres-Garrido, Marc Yeste
Basal energetic metabolism in sperm, particularly oxidative phosphorylation, is known to condition not only their oocyte fertilising ability, but also the subsequent embryo development. While the molecular pathways underlying these events still need to be elucidated, reactive oxygen species (ROS) could have a relevant role. We, therefore, aimed to describe the mechanisms through which mitochondrial
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Fibroblasts inhibit osteogenesis by regulating nuclear-cytoplasmic shuttling of YAP in mesenchymal stem cells and secreting DKK1 Biol. Res. (IF 6.7) Pub Date : 2024-01-20 Fei Huang, Guozhen Wei, Hai Wang, Ying Zhang, Wenbin Lan, Yun Xie, Gui Wu
Fibrous scars frequently form at the sites of bone nonunion when attempts to repair bone fractures have failed. However, the detailed mechanism by which fibroblasts, which are the main components of fibrous scars, impede osteogenesis remains largely unknown. In this study, we found that fibroblasts compete with osteogenesis in both human bone nonunion tissues and BMP2-induced ectopic osteogenesis in
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MSC-derived exosomes protect auditory hair cells from neomycin-induced damage via autophagy regulation Biol. Res. (IF 6.7) Pub Date : 2024-01-13 Huan Liu, Huijuan Kuang, Yiru Wang, Lili Bao, Wanxin Cao, Lu Yu, Meihao Qi, Renfeng Wang, Xiaoshan Yang, Qingyuan Ye, Feng Ding, Lili Ren, Siying Liu, Furong Ma, Shiyu Liu
Sensorineural hearing loss (SNHL) poses a major threat to both physical and mental health; however, there is still a lack of effective drugs to treat the disease. Recently, novel biological therapies, such as mesenchymal stem cells (MSCs) and their products, namely, exosomes, are showing promising therapeutic potential due to their low immunogenicity, few ethical concerns, and easy accessibility. Nevertheless
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Alpha-synuclein dynamics bridge Type-I Interferon response and SARS-CoV-2 replication in peripheral cells Biol. Res. (IF 6.7) Pub Date : 2024-01-09 Fiona Limanaqi, Silvia Zecchini, Irma Saulle, Sergio Strizzi, Claudia Vanetti, Micaela Garziano, Gioia Cappelletti, Debora Parolin, Sonia Caccia, Daria Trabattoni, Claudio Fenizia, Mario Clerici, Mara Biasin
Increasing evidence suggests a double-faceted role of alpha-synuclein (α-syn) following infection by a variety of viruses, including SARS-CoV-2. Although α-syn accumulation is known to contribute to cell toxicity and the development and/or exacerbation of neuropathological manifestations, it is also a key to sustaining anti-viral innate immunity. Consistently with α-syn aggregation as a hallmark of
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Lactadherin immunoblockade in small extracellular vesicles inhibits sEV-mediated increase of pro-metastatic capacities Biol. Res. (IF 6.7) Pub Date : 2024-01-03 Eduardo Durán-Jara, Matías del Campo, Valentina Gutiérrez, Ignacio Wichmann, César Trigo, Marcelo Ezquer, Lorena Lobos-González
Tumor-derived small extracellular vesicles (sEVs) can promote tumorigenic and metastatic capacities in less aggressive recipient cells mainly through the biomolecules in their cargo. However, despite recent advances, the specific molecules orchestrating these changes are not completely defined. Lactadherin is a secreted glycoprotein typically found in the milk fat globule membrane. Its overexpression
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Integration of ATAC-seq and RNA-seq identifies MX1-mediated AP-1 transcriptional regulation as a therapeutic target for Down syndrome Biol. Res. (IF 6.7) Pub Date : 2023-12-09 Zhenglong Guo, Yongchang Zhu, Hai Xiao, Ranran Dai, Wenke Yang, Wei Xue, Xueying Zhang, Bingtao Hao, Shixiu Liao
Growing evidence has suggested that Type I Interferon (I-IFN) plays a potential role in the pathogenesis of Down Syndrome (DS). This work investigates the underlying function of MX1, an effector gene of I-IFN, in DS-associated transcriptional regulation and phenotypic modulation. We performed assay for transposase-accessible chromatin with high-throughout sequencing (ATAC-seq) to explore the difference
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The novel roles of YULINK in the migration, proliferation and glycolysis of pulmonary arterial smooth muscle cells: implications for pulmonary arterial hypertension Biol. Res. (IF 6.7) Pub Date : 2023-12-07 Yi-Chia Wu, Wei-Ting Wang, Ming-Chun Yang, Yu-Tsun Su, Jwu-Lai Yeh, Jong-Hau Hsu, Jiunn-Ren Wu
Abnormal remodeling of the pulmonary vasculature, characterized by the proliferation and migration of pulmonary arterial smooth muscle cells (PASMCs) along with dysregulated glycolysis, is a pathognomonic feature of pulmonary arterial hypertension (PAH). YULINK (MIOS, Entrez Gene: 54468), a newly identified gene, has been recently shown to possess pleiotropic physiologic functions. This study aims
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Electroacupuncture promotes neurogenesis in the dentate gyrus and improves pattern separation in an early Alzheimer's disease mouse model Biol. Res. (IF 6.7) Pub Date : 2023-12-02 Yanyi Ding, Long Li, Sinuo Wang, Yajun Cao, Minguang Yang, Yaling Dai, Huawei Lin, Jianhong Li, Yulu Liu, Zhifu Wang, Weilin Liu, Jing Tao
Impaired pattern separation occurs in the early stage of Alzheimer’s disease (AD), and hippocampal dentate gyrus (DG) neurogenesis participates in pattern separation. Here, we investigated whether spatial memory discrimination impairment can be improved by promoting the hippocampal DG granule cell neogenesis-mediated pattern separation in the early stage of AD by electroacupuncture (EA). Five familial
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Role of SYVN1 in the control of airway remodeling in asthma protection by promoting SIRT2 ubiquitination and degradation Biol. Res. (IF 6.7) Pub Date : 2023-12-02 Bing Dai, Si Liu, Wenxin Shen, Li Chen, Qianlan Zhou, Lina Han, Qinzhen Zhang, Lishen Shan
Asthma is a heterogenous disease that characterized by airway remodeling. SYVN1 (Synoviolin 1) acts as an E3 ligase to mediate the suppression of endoplasmic reticulum (ER) stress through ubiquitination and degradation. However, the role of SYVN1 in the pathogenesis of asthma is unclear. In the present study, an ovalbumin (OVA)-induced murine model was used to evaluate the effect of SYVN1 on asthma
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Advances towards the use of gastrointestinal tumor patient-derived organoids as a therapeutic decision-making tool Biol. Res. (IF 6.7) Pub Date : 2023-12-02 Javiera Obreque, Luis Vergara-Gómez, Nicolás Venegas, Helga Weber, Gareth I. Owen, Pablo Pérez-Moreno, Pamela Leal, Juan Carlos Roa, Carolina Bizama
In December 2022 the US Food and Drug Administration (FDA) removed the requirement that drugs in development must undergo animal testing before clinical evaluation, a declaration that now demands the establishment and verification of ex vivo preclinical models that closely represent tumor complexity and that can predict therapeutic response. Fortunately, the emergence of patient-derived organoid (PDOs)
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Melatonin alleviates pyroptosis by regulating the SIRT3/FOXO3α/ROS axis and interacting with apoptosis in Atherosclerosis progression Biol. Res. (IF 6.7) Pub Date : 2023-12-02 Lin Cong, Xiankun Liu, Yiming Bai, Qin Qin, Lili Zhao, Ying Shi, Yunpeng Bai, Zhigang Guo
Atherosclerosis (AS), a significant contributor to cardiovascular disease (CVD), is steadily rising with the aging of the global population. Pyroptosis and apoptosis, both caspase-mediated cell death mechanisms, play an essential role in the occurrence and progression of AS. The human pineal gland primarily produces melatonin (MT), an indoleamine hormone with powerful anti-oxidative, anti-pyroptotic
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Prenatal ethanol exposure and changes in fetal neuroendocrine metabolic programming Biol. Res. (IF 6.7) Pub Date : 2023-11-17 Liang Liu, Yinxian Wen, Qubo Ni, Liaobin Chen, Hui Wang
Prenatal ethanol exposure (PEE) (mainly through maternal alcohol consumption) has become widespread. However, studies suggest that it can cause intrauterine growth retardation (IUGR) and multi-organ developmental toxicity in offspring, and susceptibility to various chronic diseases (such as neuropsychiatric diseases, metabolic syndrome, and related diseases) in adults. Through ethanol’s direct effects
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Autologous non-invasively derived stem cells mitochondria transfer shows therapeutic advantages in human embryo quality rescue Biol. Res. (IF 6.7) Pub Date : 2023-11-17 Zhixin Jiang, Cheng Shi, Hongjing Han, Min Fu, Honglan Zhu, Tingting Han, Jia Fei, Yining Huang, Zhiping Jin, Jianan He, Yanbin Wang, Xi Chen, Huan Shen
The decline in the quantity and quality of mitochondria are closely associated with infertility, particularly in advanced maternal age. Transferring autologous mitochondria into the oocytes of infertile females represents an innovative and viable strategy for treating infertility, with no concerns regarding ethical considerations. As the donor cells of mitochondria, stem cells have biological advantages
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Development of synthetic modulator enabling long-term propagation and neurogenesis of human embryonic stem cell-derived neural progenitor cells Biol. Res. (IF 6.7) Pub Date : 2023-11-11 Ceheng Liao, Ying Guan, Jihui Zheng, Xue Wang, Meixia Wang, Zhouhai Zhu, Qiyuan Peng, Hong-Hui Wang, Meng Li
Neural progenitor cells (NPCs) are essential for in vitro drug screening and cell-based therapies for brain-related disorders, necessitating well-defined and reproducible culture systems. Current strategies employing protein growth factors pose challenges in terms of both reproducibility and cost. In this study, we developed a novel DNA-based modulator to regulate FGFR signaling in NPCs, thereby facilitating
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Heat-responsive microRNAs participate in regulating the pollen fertility stability of CMS-D2 restorer line under high-temperature stress Biol. Res. (IF 6.7) Pub Date : 2023-11-09 Meng Zhang, Xuexian Zhang, Ruijie Wang, Rong Zang, Liping Guo, Tingxiang Qi, Huini Tang, Liangliang Chen, Hailin Wang, Xiuqin Qiao, Jianyong Wu, Chaozhu Xing
Anther development and pollen fertility of cytoplasmic male sterility (CMS) conditioned by Gossypium harknessii cytoplasm (CMS-D2) restorer lines are susceptible to continuous high-temperature (HT) stress in summer, which seriously hinders the large-scale application of "three-line" hybrids in production. Here, integrated small RNA, transcriptome, degradome, and hormone profiling was performed to explore
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Chemogenetic inhibition of NTS astrocytes normalizes cardiac autonomic control and ameliorate hypertension during chronic intermittent hypoxia Biol. Res. (IF 6.7) Pub Date : 2023-11-06 Katherin Pereyra, Alexandra Las Heras, Camilo Toledo, Esteban Díaz-Jara, Rodrigo Iturriaga, Rodrigo Del Rio
Obstructive sleep apnea (OSA) is characterized by recurrent episodes of chronic intermittent hypoxia (CIH), which has been linked to the development of sympathoexcitation and hypertension. Furthermore, it has been shown that CIH induced inflammation and neuronal hyperactivation in the nucleus of the solitary tract (NTS), a key brainstem region involved in sympathetic and cardiovascular regulation.
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SARS-CoV-2 spike protein S1 activates Cx43 hemichannels and disturbs intracellular Ca2+ dynamics Biol. Res. (IF 6.7) Pub Date : 2023-10-25 Juan Prieto-Villalobos, Claudia M. Lucero, Maximiliano Rovegno, Gonzalo I. Gómez, Mauricio A. Retamal, Juan A. Orellana
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes the ongoing coronavirus disease 2019 (COVID-19). An aspect of high uncertainty is whether the SARS-CoV-2 per se or the systemic inflammation induced by viral infection directly affects cellular function and survival in different tissues. It has been postulated that tissue dysfunction and damage observed in COVID-19 patients may rely
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The effect of zofenopril on the cardiovascular system of spontaneously hypertensive rats treated with the ACE2 inhibitor MLN-4760 Biol. Res. (IF 6.7) Pub Date : 2023-10-25 Sona Cacanyiova, Martina Cebova, Fedor Simko, Tomas Baka, Iveta Bernatova, Michal Kluknavsky, Stefan Zorad, Katarina Krskova, Ezgi Shaman, Anna Zemancikova, Andrej Barta, Basak G. Aydemir, Andrea Berenyiova
Angiotensin converting enzyme 2 (ACE2) plays a crucial role in the infection cycle of SARS-CoV-2 responsible for formation of COVID-19 pandemic. In the cardiovascular system, the virus enters the cells by binding to the transmembrane form of ACE2 causing detrimental effects especially in individuals with developed hypertension or heart disease. Zofenopril, a H2S-releasing angiotensin-converting enzyme
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Two murine models of sepsis: immunopathological differences between the sexes—possible role of TGFβ1 in female resistance to endotoxemia Biol. Res. (IF 6.7) Pub Date : 2023-10-24 Rafael Bojalil, Armando Ruíz-Hernández, Arturo Villanueva-Arias, Luis Manuel Amezcua-Guerra, Sergio Cásarez-Alvarado, Ana María Hernández-Dueñas, Verónica Rodríguez-Galicia, Lenin Pavón, Brenda Marquina, Enrique Becerril-Villanueva, Rogelio Hernández-Pando, Ricardo Márquez-Velasco
Endotoxic shock (ExSh) and cecal ligature and puncture (CLP) are models that induce sepsis. In this work, we investigated early immunologic and histopathologic changes induced by ExSh or CLP models in female and male mice. Remarkable results showed that females supported twice the LD100 of LPS for males, CLP survival and CFU counts were similar between genders, high circulating LPS levels in ExSh mice
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An intracellular, non-oxidative factor activates in vitro chromatin fragmentation in pig sperm Biol. Res. (IF 6.7) Pub Date : 2023-10-24 Estel Viñolas-Vergés, Marc Yeste, Ferran Garriga, Sergi Bonet, Yentel Mateo-Otero, Jordi Ribas-Maynou
In vitro incubation of epididymal and vas deferens sperm with Mn2+ induces Sperm Chromatin Fragmentation (SCF), a mechanism that causes double-stranded breaks in toroid-linker regions (TLRs). Whether this mechanism, thought to require the participation of topoisomerases and/or DNAses and thus far only described in epididymal mouse sperm, can be triggered in ejaculated sperm is yet to be elucidated
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Focal ischemic stroke modifies microglia-derived exosomal miRNAs: potential role of mir-212-5p in neuronal protection and functional recovery Biol. Res. (IF 6.7) Pub Date : 2023-10-03 Si-si Li, Jia-jia Wu, Xiang-xin Xing, Yu-lin Li, Jie Ma, Yu-jie Duan, Jun-peng Zhang, Chun-lei Shan, Xu-yun Hua, Mou-xiong Zheng, Jian-guang Xu
Ischemic stroke is a severe type of stroke with high disability and mortality rates. In recent years, microglial exosome-derived miRNAs have been shown to be promising candidates for the treatment of ischemic brain injury and exert neuroprotective effects. Mechanisms underlying miRNA dysregulation in ischemic stroke are still being explored. Here, we aimed to verify whether miRNAs derived from exosomes
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S-Nitrosylation in endothelial cells contributes to tumor cell adhesion and extravasation during breast cancer metastasis Biol. Res. (IF 6.7) Pub Date : 2023-09-29 T. Koning, F. Cordova, G. Aguilar, J. Sarmiento, G. A. Mardones, M. Boric, M. Varas-Godoy, A. Lladser, W. N. Duran, P. Ehrenfeld, F. A. Sanchez
Nitric oxide is produced by different nitric oxide synthases isoforms. NO activates two signaling pathways, one dependent on soluble guanylate cyclase and protein kinase G, and other where NO post-translationally modifies proteins through S-nitrosylation, which is the modification induced by NO in free-thiol cysteines in proteins to form S-nitrosothiols. High levels of NO have been detected in blood
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Identifying pyroptosis- and inflammation-related genes in intracranial aneurysms based on bioinformatics analysis Biol. Res. (IF 6.7) Pub Date : 2023-09-27 Donglin Zhou, Yimin Zhu, Peng Jiang, Tongfu Zhang, Jianfeng Zhuang, Tao Li, Linzeng Qi, Yunyan Wang
Intracranial aneurysm (IA) is the most common cerebrovascular disease, and subarachnoid hemorrhage caused by its rupture can seriously impede nerve function. Pyroptosis is an inflammatory mode of cell death whose underlying mechanisms involving the occurrence and rupture of IAs remain unclear. In this study, using bioinformatics analysis, we identified the potential pyroptosis-related genes (PRGs)
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Drosophila Atlastin regulates synaptic vesicle mobilization independent of bone morphogenetic protein signaling Biol. Res. (IF 6.7) Pub Date : 2023-09-14 Francisca Bertin, Jorge Jara-Wilde, Benedikt Auer, Andrés Köhler-Solís, Carolina González-Silva, Ulrich Thomas, Jimena Sierralta
The endoplasmic reticulum (ER) contacts endosomes in all parts of a motor neuron, including the axon and presynaptic terminal, to move structural proteins, proteins that send signals, and lipids over long distances. Atlastin (Atl), a large GTPase, is required for membrane fusion and the structural dynamics of the ER tubules. Atl mutations are the second most common cause of Hereditary Spastic Paraplegia
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Mucin1 induced trophoblast dysfunction in gestational diabetes mellitus via Wnt/β-catenin pathway Biol. Res. (IF 6.7) Pub Date : 2023-08-22 Shuang-Shuang Cui, Ping Zhang, Lu Sun, Yu-Lin-Lan Yuan, Jingyun Wang, Feng-Xiang Zhang, Ruiman Li
To elucidate the role of Mucin1 (MUC1) in the trophoblast function (glucose uptake and apoptosis) of gestational diabetes mellitus (GDM) women through the Wnt/β-catenin pathway. Glucose uptake was analyzed by plasma GLUT1 and GLUT4 levels with ELISA and measured by the expression of GLUT4 and INSR with immunofluorescence and Western blotting. Apoptosis was measured by the expression of Bcl-2 and Caspase3
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Human umbilical cord mesenchymal stem cells (hUC-MSCs) alleviate paclitaxel-induced spermatogenesis defects and maintain male fertility Biol. Res. (IF 6.7) Pub Date : 2023-08-13 YuSheng Zhang, YaNan Liu, Zi Teng, ZeLin Wang, Peng Zhu, ZhiXin Wang, FuJun Liu, XueXia Liu
Chemotherapeutic drugs can cause reproductive damage by affecting sperm quality and other aspects of male fertility. Stem cells are thought to alleviate the damage caused by chemotherapy drugs and to play roles in reproductive protection and treatment. This study aimed to explore the effects of human umbilical cord mesenchymal stem cells (hUC-MSCs) on alleviating paclitaxel (PTX)-induced spermatogenesis
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Exploring the Neandertal legacy of pancreatic ductal adenocarcinoma risk in Eurasians Biol. Res. (IF 6.7) Pub Date : 2023-08-13 Margherita Piccardi, Manuel Gentiluomo, Stefania Bertoncini, Raffaele Pezzilli, Bálint Erőss, Stefania Bunduc, Faik G. Uzunoglu, Renata Talar-Wojnarowska, Tomas Vanagas, Cosimo Sperti, Martin Oliverius, Mateus Nóbrega Aoki, Stefano Ermini, Tamás Hussein, Ugo Boggi, Krzysztof Jamroziak, Evaristo Maiello, Luca Morelli, Ludmila Vodickova, Gregorio Di Franco, Stefano Landi, Andrea Szentesi, Martin Lovecek
The genomes of present-day non-Africans are composed of 1–3% of Neandertal-derived DNA as a consequence of admixture events between Neandertals and anatomically modern humans about 50–60 thousand years ago. Neandertal-introgressed single nucleotide polymorphisms (aSNPs) have been associated with modern human disease-related traits, which are risk factors for pancreatic ductal adenocarcinoma (PDAC)
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Identification and analysis of key hypoxia- and immune-related genes in hypertrophic cardiomyopathy Biol. Res. (IF 6.7) Pub Date : 2023-08-09 Haozhen Yu, Lanxin Gu, Linfang Du, Zhao Dong, Zhuang Li, Mujun Yu, Yue Yin, Yishi Wang, Lu Yu, Heng Ma
Hypertrophic cardiomyopathy (HCM), an autosomal dominant genetic disease, is the main cause of sudden death in adolescents and athletes globally. Hypoxia and immune factors have been revealed to be related to the pathology of HCM. There is growing evidence of a role for hypoxia and inflammation as triggers and enhancers in the pathology in HCM. However, the role of hypoxia- and immune-related genes
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How do prolonged anchorage-free lifetimes strengthen non-small-cell lung cancer cells to evade anoikis? – A link with altered cellular metabolomics Biol. Res. (IF 6.7) Pub Date : 2023-08-05 Rungroch Sungthong, Hnin Ei Ei Khine, Somruethai Sumkhemthong, Pithi Chanvorachote, Rossarin Tansawat, Chatchai Chaotham
Malignant cells adopt anoikis resistance to survive anchorage-free stresses and initiate cancer metastasis. It is still unknown how varying periods of anchorage loss contribute to anoikis resistance, cell migration, and metabolic reprogramming of cancerous cells. Our study demonstrated that prolonging the anchorage-free lifetime of non-small-cell lung cancer NCI-H460 cells for 7 days strengthened anoikis
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Single nucleotide polymorphisms associated with wine fermentation and adaptation to nitrogen limitation in wild and domesticated yeast strains Biol. Res. (IF 6.7) Pub Date : 2023-07-29 Eduardo I. Kessi-Pérez, Eric Acuña, Camila Bastías, Leyanis Fundora, Manuel Villalobos-Cid, Andrés Romero, Sakshi Khaiwal, Matteo De Chiara, Gianni Liti, Francisco Salinas, Claudio Martínez
For more than 20 years, Saccharomyces cerevisiae has served as a model organism for genetic studies and molecular biology, as well as a platform for biotechnology (e.g., wine production). One of the important ecological niches of this yeast that has been extensively studied is wine fermentation, a complex microbiological process in which S. cerevisiae faces various stresses such as limited availability
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Investigating the dark-side of the genome: a barrier to human disease variant discovery? Biol. Res. (IF 6.7) Pub Date : 2023-07-20 Niamh M. Ryan, Aiden Corvin
The human genome contains regions that cannot be adequately assembled or aligned using next generation short-read sequencing technologies. More than 2500 genes are known contain such ‘dark’ regions. In this study, we investigate the negative consequences of dark regions on gene discovery across a range of disease and study types, showing that dark regions are likely preventing researchers from identifying
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Hyperbaric oxygen treatment increases intestinal stem cell proliferation through the mTORC1/S6K1 signaling pathway in Mus musculus Biol. Res. (IF 6.7) Pub Date : 2023-07-13 Ignacio Casanova-Maldonado, David Arancibia, Pablo Lois, Isaac Peña-Villalobos, Verónica Palma
Hyperbaric oxygen treatment (HBOT) has been reported to modulate the proliferation of neural and mesenchymal stem cell populations, but the molecular mechanisms underlying these effects are not completely understood. In this study, we aimed to assess HBOT somatic stem cell modulation by evaluating the role of the mTOR complex 1 (mTORC1), a key regulator of cell metabolism whose activity is modified
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Polar microalgae extracts protect human HaCaT keratinocytes from damaging stimuli and ameliorate psoriatic skin inflammation in mice Biol. Res. (IF 6.7) Pub Date : 2023-07-13 YoonHee Lim, So-Hyun Park, Eun Jae Kim, HeeJun Lim, Jinsun Jang, In-Sun Hong, Sanghee Kim, YunJae Jung
Polar microalgae contain unique compounds that enable them to adapt to extreme environments. As the skin barrier is our first line of defense against external threats, polar microalgae extracts may possess restorative properties for damaged skin, but the potential of microalgae extracts as skin protective agents remains unknown. This study aimed to analyze compound profiles from polar microalgae extracts
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Correction: Utility of melatonin in mitigating ionizing radiation‑induced testis injury through synergistic interdependence of its biological properties Biol. Res. (IF 6.7) Pub Date : 2023-07-12 Maggie E. Amer, Azza I. Othman, Hajer Mohammed Abozaid, Mohamed A. El‑Missiry
Correction: Biological Research (2022) 55:33 https://doi.org/10.1186/s40659-022-00401-6 Following publication of the original article [1], the authors identified an error in Figs. 3D and 4. The correct version of Figs. 3D and 4 are provided in this correction. Fig. 3 I and II Histopathological changes in rat’s testes of the control and different treatment groups after 3 and 14 days of irradiation and
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Beyond energy provider: multifunction of lipid droplets in embryonic development Biol. Res. (IF 6.7) Pub Date : 2023-07-12 Tai Li, Yi Jin, Jian Wu, Zhuqing Ren
Since the discovery, lipid droplets (LDs) have been recognized to be sites of cellular energy reserves, providing energy when necessary to sustain cellular life activities. Many studies have reported large numbers of LDs in eggs and early embryos from insects to mammals. The questions of how LDs are formed, what role they play, and what their significance is for embryonic development have been attracting
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Retraction Note: Tridax procumbens flavonoids: a prospective bioactive compound increased osteoblast differentiation and trabecular bone formation Biol. Res. (IF 6.7) Pub Date : 2023-07-07 Md. Abdullah Al Mamun, Mohammad Jakir Hosen, Amina Khatun, M. Masihul Alam, Md. Abdul Alim Al‑Bari
Retraction : Biol Res (2017) 50:28 https://doi.org/10.1186/s40659-017-0134-7 The Editor in Chief has retracted this article because of significant text overlap with a previous work by the same authors [1]. Further investigation by the publisher found that images in Figure 5 appear to be all derived from the same picture with some small differences in brightness, field of view or magnification, but
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Electroacupuncture protective effects after cerebral ischemia are mediated through miR-219a inhibition Biol. Res. (IF 6.7) Pub Date : 2023-06-30 Yaling Dai, Sinuo Wang, Minguang Yang, Peiyuan Zhuo, Yanyi Ding, Xiaoling Li, Yajun Cao, Xiaoqin Guo, Huawei Lin, Jing Tao, Lidian Chen, Weilin Liu
Electroacupuncture (EA) is a complementary and alternative therapy which has shown protective effects on vascular cognitive impairment (VCI). However, the underlying mechanisms are not entirely understood. Rat models of VCI were established with cerebral ischemia using occlusion of the middle cerebral artery or bilateral common carotid artery. The brain structure and function imaging were measured
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Topsoil and subsoil bacterial community assemblies across different drainage conditions in a mountain environment Biol. Res. (IF 6.7) Pub Date : 2023-06-24 Constanza Aguado-Norese, Valentina Cárdenas, Alexis Gaete, Dinka Mandakovic, Javiera Vasquez-Dean, Christian Hodar, Marco Pfeiffer, Mauricio Gonzalez
High mountainous environments are of particular interest as they play an essential role for life and human societies, while being environments which are highly vulnerable to climate change and land use intensification. Despite this, our knowledge of high mountain soils in South America and their microbial community structure is strikingly scarce, which is of more concern considering the large population
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Functional defects in hiPSCs-derived cardiomyocytes from patients with a PLEKHM2-mutation associated with dilated cardiomyopathy and left ventricular non-compaction Biol. Res. (IF 6.7) Pub Date : 2023-06-23 Nataly Korover, Sharon Etzion, Alexander Cherniak, Tatiana Rabinski, Aviva Levitas, Yoram Etzion, Rivka Ofir, Ruti Parvari, Smadar Cohen
Dilated cardiomyopathy (DCM) is a primary myocardial disease, leading to heart failure and excessive risk of sudden cardiac death with rather poorly understood pathophysiology. In 2015, Parvari's group identified a recessive mutation in the autophagy regulator, PLEKHM2 gene, in a family with severe recessive DCM and left ventricular non-compaction (LVNC). Fibroblasts isolated from these patients exhibited
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Human VDAC pseudogenes: an emerging role for VDAC1P8 pseudogene in acute myeloid leukemia Biol. Res. (IF 6.7) Pub Date : 2023-06-22 Xena Giada Pappalardo, Pierpaolo Risiglione, Federica Zinghirino, Angela Ostuni, Daniela Luciano, Faustino Bisaccia, Vito De Pinto, Francesca Guarino, Angela Messina
Voltage-dependent anion selective channels (VDACs) are the most abundant mitochondrial outer membrane proteins, encoded in mammals by three genes, VDAC1, 2 and 3, mostly ubiquitously expressed. As 'mitochondrial gatekeepers', VDACs control organelle and cell metabolism and are involved in many diseases. Despite the presence of numerous VDAC pseudogenes in the human genome, their significance and possible
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ABCA1 transporter promotes the motility of human melanoma cells by modulating their plasma membrane organization Biol. Res. (IF 6.7) Pub Date : 2023-06-13 Ambroise Wu, Ewa Mazurkiewicz, Piotr Donizy, Krzysztof Kotowski, Małgorzata Pieniazek, Antonina J. Mazur, Aleksander Czogalla, Tomasz Trombik
Melanoma is one of the most aggressive and deadliest skin tumor. Cholesterol content in melanoma cells is elevated, and a portion of it accumulates into lipid rafts. Therefore, the plasma membrane cholesterol and its lateral organization might be directly linked with tumor development. ATP Binding Cassette A1 (ABCA1) transporter modulates physico-chemical properties of the plasma membrane by modifying
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Acupuncture regulates the apoptosis of ovarian granulosa cells in polycystic ovarian syndrome-related abnormal follicular development through LncMEG3-mediated inhibition of miR-21-3p Biol. Res. (IF 6.7) Pub Date : 2023-06-12 Xiaohong Chen, Hengzhen He, Bingcai Long, Binli Wei, Peng Yang, Xiaoying Huang, Qian Wang, Jing Lin, Hongliang Tang
The main features of polycystic ovary syndrome (PCOS) are abnormal follicular development and ovulatory dysfunction, which are caused by excessive apoptosis of ovarian granulosa cells. Acupuncture has been shown to improve follicular development abnormalities in patients with PCOS, but its mechanism is unknown. This study hypothesized that the mechanism of acupuncture on follicular development abnormalities
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Cholic and deoxycholic acids induce mitochondrial dysfunction, impaired biogenesis and autophagic flux in skeletal muscle cells Biol. Res. (IF 6.7) Pub Date : 2023-06-08 Johanna Abrigo, Hugo Olguín, Franco Tacchi, Josué Orozco-Aguilar, Mayalen Valero-Breton, Jorge Soto, Mauricio Castro-Sepúlveda, Alvaro A. Elorza, Felipe Simon, Claudio Cabello-Verrugio
Skeletal muscle is sensitive to bile acids (BA) because it expresses the TGR5 receptor for BA. Cholic (CA) and deoxycholic (DCA) acids induce a sarcopenia-like phenotype through TGR5-dependent mechanisms. Besides, a mouse model of cholestasis-induced sarcopenia was characterised by increased levels of serum BA and muscle weakness, alterations that are dependent on TGR5 expression. Mitochondrial alterations
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iPSCs ameliorate hypoxia-induced autophagy and atrophy in C2C12 myotubes via the AMPK/ULK1 pathway Biol. Res. (IF 6.7) Pub Date : 2023-06-03 Haimei Cen, Pin Fan, Yuting Ding, Bin Luo, Hong Luo, Menglong Chen, Yu Zhang
Duchenne muscular dystrophy (DMD) is an X-linked lethal genetic disorder for which there is no effective treatment. Previous studies have shown that stem cell transplantation into mdx mice can promote muscle regeneration and improve muscle function, however, the specific molecular mechanisms remain unclear. DMD suffers varying degrees of hypoxic damage during disease progression. This study aimed to
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Ursodeoxycholic acid induces sarcopenia associated with decreased protein synthesis and autophagic flux Biol. Res. (IF 6.7) Pub Date : 2023-05-27 Josué Orozco-Aguilar, Franco Tacchi, Francisco Aguirre, Mayalen Valero-Breton, Mauricio Castro-Sepulveda, Felipe Simon, Claudio Cabello-Verrugio
Skeletal muscle generates force and movements and maintains posture. Under pathological conditions, muscle fibers suffer an imbalance in protein synthesis/degradation. This event causes muscle mass loss and decreased strength and muscle function, a syndrome known as sarcopenia. Recently, our laboratory described secondary sarcopenia in a chronic cholestatic liver disease (CCLD) mouse model. Interestingly
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Neuroprotective effects of the PPARβ/δ antagonist GSK0660 in in vitro and in vivo Parkinson’s disease models Biol. Res. (IF 6.7) Pub Date : 2023-05-25 Andrea Antonosante, Vanessa Castelli, Martina Sette, Margherita Alfonsetti, Mariano Catanesi, Elisabetta Benedetti, Matteo Ardini, Annamaria Cimini, Michele d’Angelo
The underlying mechanism of Parkinson’s disease are still unidentified, but excitotoxicity, oxidative stress, and neuroinflammation are considered key actors. Proliferator activated receptors (PPARs) are transcription factors involved in the control of numerous pathways. Specifically, PPARβ/δ is recognized as an oxidative stress sensor, and we have previously reported that it plays a detrimental role
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Electroacupuncture inhibits dendritic spine remodeling through the srGAP3-Rac1 signaling pathway in rats with SNL Biol. Res. (IF 6.7) Pub Date : 2023-05-22 Qiaoyun Wu, Chenchen Cai, Xinwang Ying, Yujun Zheng, Jiaying Yu, Xiaoxue Gu, Wenzhan Tu, Xinfa Lou, Guanhu Yang, Ming Li, Songhe Jiang
Previous studies have shown that peripheral nerve injury can lead to abnormal dendritic spine remodeling in spinal dorsal horn neurons. Inhibition of abnormal dendritic spine remodeling can relieve neuropathic pain. Electroacupuncture (EA) has a beneficial effect on the treatment of neuropathic pain, but the specific mechanism remains unclear. Evidence has shown that slit-robo GTPase activating protein
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Activation of a hypothalamus-habenula circuit by mechanical stimulation inhibits cocaine addiction-like behaviors Biol. Res. (IF 6.7) Pub Date : 2023-05-17 Han Byeol Jang, DanBi Ahn, Suchan Chang, Hyung Kyu Kim, Bong Hyo Lee, Sang Chan Kim, Scott C. Steffensen, Kyle B. Bills, Hubert Lee, Hee Young Kim
Mechanoreceptor activation modulates GABA neuron firing and dopamine (DA) release in the mesolimbic DA system, an area implicated in reward and substance abuse. The lateral habenula (LHb), the lateral hypothalamus (LH), and the mesolimbic DA system are not only reciprocally connected, but also involved in drug reward. We explored the effects of mechanical stimulation (MS) on cocaine addiction-like
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Correction: Disseminated intravascular coagulation phenotype is regulated by the TRPM7 channel during sepsis Biol. Res. (IF 6.7) Pub Date : 2023-05-11 Ivanka Jiménez-Dinamarca, Yolanda Prado, Pablo Tapia, Sebastian Gatica, Clemens Alt, Charles P. Lin, Cristian Reyes-Martínez, Carmen G. Feijóo, Cristobal Aravena, Alejandra González-Canacer, Simón Correa, Diego Varela, Claudio Cabello-Verrugio, Felipe Simon
Correction: Biological Research (2023) 56:8 https://doi.org/10.1186/s40659-023-00419-4 Following publication of the original article [1], the authors identified an error in Figure 4. The original image in the panel H was inadvertently substituted by a copy of the image showed in panel I (just slightly moved to the left). In the corrected Fig. 4, panel H was changed by the original image. Arrowheads
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Maintenance of chronicity signatures in fibroblasts isolated from recessive dystrophic epidermolysis bullosa chronic wound dressings under culture conditions Biol. Res. (IF 6.7) Pub Date : 2023-05-10 Cristian De Gregorio, Evelyng Catalán, Gabriel Garrido, Pilar Morandé, Jimena Castillo Bennett, Catalina Muñoz, Glenda Cofré, Ya-Lin Huang, Bárbara Cuadra, Paola Murgas, Margarita Calvo, Fernando Altermatt, María Joao Yubero, Francis Palisson, Andrew P. South, Marcelo Ezquer, Ignacia Fuentes
Recessive Dystrophic Epidermolysis Bullosa (RDEB) is a rare inherited skin disease caused by variants in the COL7A1 gene, coding for type VII collagen (C7), an important component of anchoring fibrils in the basement membrane of the epidermis. RDEB patients suffer from skin fragility starting with blister formation and evolving into chronic wounds, inflammation and skin fibrosis, with a high risk of
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Conformational characterization of the mammalian-expressed SARS-CoV-2 recombinant receptor binding domain, a COVID-19 vaccine Biol. Res. (IF 6.7) Pub Date : 2023-05-08 Leina Moro-Pérez, Tammy Boggiano-Ayo, Sum Lai Lozada-Chang, Olga Lidia Fernández-Saiz, Kathya Rashida de la Luz, Jose Alberto Gómez-Pérez
The COVID-19 pandemic has caused a large number of diseases worldwide. There are few vaccines to constrain this disease and the value of them is high. In this sense, the antigens of the vaccine platform Soberana, the receptor binding domain from SARS-CoV-2 Spike protein, both the monomeric (mRBD) and dimeric (dRBD) forms, have been developed. This study encompassed several analyses by different techniques
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NEDD4-1 deficiency impairs satellite cell function during skeletal muscle regeneration Biol. Res. (IF 6.7) Pub Date : 2023-05-05 Felipe Cabezas, Claudio Cabello-Verrugio, Natalia González, Jeremy Salas, Manuel J. Ramírez, Eduardo de la Vega, Hugo C. Olguín
Satellite cells are tissue-specific stem cells primarily responsible for the regenerative capacity of skeletal muscle. Satellite cell function and maintenance are regulated by extrinsic and intrinsic mechanisms, including the ubiquitin–proteasome system, which is key for maintaining protein homeostasis. In this context, it has been shown that ubiquitin-ligase NEDD4-1 targets the transcription factor
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Propofol inhibits myocardial injury induced by microvesicles derived from hypoxia-reoxygenated endothelial cells via lncCCT4-2/CCT4 signaling Biol. Res. (IF 6.7) Pub Date : 2023-05-05 Xiaojun Zhang, Changsen Wang, Hao Xu, Shuyun Cai, Keyu Liu, Simeng Li, Linming Chen, Siman Shen, Xiaoxia Gu, Jing Tang, Zhengyuan Xia, Zhe Hu, Xiaotang Ma, Liangqing Zhang
Ischemia-reperfusion (IR) induces increased release of extracellular vesicles in the heart and exacerbates myocardial IR injury. We have previously shown that propofol attenuates hypoxia/reoxygenation (HR)-induced injury in human umbilical vein endothelial cells (HUVECs) and that microvesicles derived from propofol-treated HUVECs inhibit oxidative stress in endothelial cells. However, the role of microvesicles
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Zebrafish ambra1b knockout reveals a novel role for Ambra1 in primordial germ cells survival, sex differentiation and reproduction Biol. Res. (IF 6.7) Pub Date : 2023-04-28 Camilla Maria Fontana, Francesca Terrin, Nicola Facchinello, Giacomo Meneghetti, Alberto Dinarello, Lisa Gambarotto, Annalisa Zuccarotto, Micol Caichiolo, Ginevra Brocca, Ranieri Verin, Francesca Nazio, Oliana Carnevali, Francesco Cecconi, Paolo Bonaldo, Luisa Dalla Valle
AMBRA1 is an intrinsically disordered protein, working as a scaffold molecule to coordinate, by protein-protein interaction, many cellular processes, including autophagy, mitophagy, apoptosis and cell cycle progression. The zebrafish genome contains two ambra1 paralogous genes (a and b), both involved in development and expressed at high levels in the gonads. Characterization of the zebrafish paralogous
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In situ isolation of nuclei or nuclear proteins from adherent cells: a simple, effective method with less cytoplasmic contamination Biol. Res. (IF 6.7) Pub Date : 2023-04-21 Ying Qin, Yun Zhou, Kun Wang, Jiaxuan Gu, Zhihao Xiong, Wendiao Zhang, Yong Chen
Isolation of nuclei or nuclear proteins is a prerequisite for western blot, nuclear proteome profiling, and other evaluations of nuclear proteins. Here, we developed a simple method for in situ isolation of nuclei or nuclear proteins by in situ removing the extranuclear part of adherent cells via a classical nonionic detergent triton X-100. First, the feasibility of our method was confirmed by confocal