-
Low-temperature extracts of Purple blossoms of basil (Ocimum basilicum L.) intervened mitochondrial translocation contributes prompted apoptosis in human breast cancer cells Biol. Res. (IF 3.092) Pub Date : 2021-01-06 Mariam Abdulaziz Alkhateeb; Wedad Refaiea Al-Otaibi; Qwait AlGabbani; Amena Ali Alsakran; Alaa Ahmed Alnafjan; Amani Mohammed Alotaibi; Wedad Saeed Al-Qahtani
The preventive and therapeutic medical utilization of this plant is an age-long practice across the globe. This study aimed to validate the impact of dark purple blossoms of basil (Ocimum basilicum L.) aqueous extract at low temperature (0 °C) mediated mitochondrial fission contributed to induced apoptosis in human breast cancer cells. Fresh blossoms were extracted at low temperature (0 °C) using a
-
Influence of kaolin application on most important fruit and leaf characteristics of two apple cultivars under sustained deficit irrigation Biol. Res. (IF 3.092) Pub Date : 2021-01-06 Somayeh Faghih; Zabihollah Zamani; Reza Fatahi; Mohammad Omidi
Apple is one of the oldest and most valuable fruits. Water restriction is one of the major problems in the production of this fruit in some planting areas. Effects of kaolin spray treatments were studied on two early apple cultivars of Golab and Shafi-Abadi under sustained deficit irrigation (SDI) in Alborz province, Iran during 2017 and 2018. Irrigation treatments were 100%, 85%, and 70% ETc and kaolin
-
Tauroursodeoxycholic acid attenuates neuronal apoptosis via the TGR5/ SIRT3 pathway after subarachnoid hemorrhage in rats Biol. Res. (IF 3.092) Pub Date : 2020-12-01 Huihui Wu; Nini Yu; Xia Wang; Yina Yang; Hui Liang
Neuronal apoptosis plays a critical event in the pathogenesis of early brain injury after subarachnoid hemorrhage (SAH). This study investigated the roles of Tauroursodeoxycholic acid (TUDCA) in attenuate neuronal apoptosis and underlying mechanisms after SAH. Sprague–Dawley rats were subjected to model of SAH and TUDCA was administered via the internal carotid injection. Small interfering RNA (siRNA)
-
Improvement of endothelial function by Gunnera tinctoria extract with antioxidant properties Biol. Res. (IF 3.092) Pub Date : 2020-11-23 Constanza Sabando; Maité Rodríguez-Díaz; Walther Ide; Edgar Pastene; Marcia Avello; Mario Simirgiotis; Susana Rojas; Enrique Villarroel; Tiare Silva-Grecchi; Cristian Gutiérrez; Rebeca Bouza; Bárbara Cicchelli; Marcelo González; Saddys Rodríguez-Llamazares
Gunnera tinctoria has been collected by Mapuche-Pewenche people for food and medicinal purposes. The high polyphenol content of methanolic extract from G. tinctoria leaves with chemical constituents such as ellagic acid and quercetin derivatives suggests its application to prevent endothelial dysfunction and oxidative stress. The aim of this study was to provide evidence of the protective effect of
-
Actin filaments mediated root growth inhibition by changing their distribution under UV-B and hydrogen peroxide exposure in Arabidopsis Biol. Res. (IF 3.092) Pub Date : 2020-11-23 Meiting Du; Yanhong Wang; Huize Chen; Rong Han
UV-B signaling in plants is mediated by UVR8, which interacts with transcriptional factors to induce root morphogenesis. However, research on the downstream molecules of UVR8 signaling in roots is still scarce. As a wide range of functional cytoskeletons, how actin filaments respond to UV-B-induced root morphogenesis has not been reported. The aim of this study was to investigate the effect of actin
-
Knockdown of lncRNA TUG1 inhibits hippocampal neuronal apoptosis and participates in aerobic exercise-alleviated vascular cognitive impairment Biol. Res. (IF 3.092) Pub Date : 2020-11-19 Jing Wang; Yali Niu; Huaying Tao; Mina Xue; Chunxiao Wan
Our previous study indicated that aerobic exercise relieves cognitive impairment in patients with vascular cognitive impairment (VCI) via regulating brain-derived neurotrophic factor (BDNF), but the mechanism is not yet clear. This study aimed to explore whether lncRNA taurine upregulated gene 1 (TUG1) participates in the process of VCI by regulating BDNF. The expressions of TUG1 and BDNF in the serum
-
Caspase-7 deficiency in Chinese hamster ovary cells reduces cell proliferation and viability Biol. Res. (IF 3.092) Pub Date : 2020-11-13 Fatemeh Safari; Safar Farajnia; Abbas Behzad Behbahani; Habib Zarredar; Mazyar Barekati-Mowahed; Hesam Dehghani
Chinese hamster ovary (CHO) cells are the most commonly used mammalian host cell in the commercial-scale production of biopharmaceutical proteins. Modification of genes involved in apoptosis may improve the productivity of CHO cells. Executive caspases, including caspases 3 and 7, play critical roles in apoptosis. The effects of the ablation of the caspase 7 gene on proliferation and viability of CHO
-
Short-term perinatal oxygen exposure may impair lung development in adult mice Biol. Res. (IF 3.092) Pub Date : 2020-11-10 Vasantha H. S. Kumar; Huamei Wang; Lori Nielsen
Hyperoxia at resuscitation increases oxidative stress, and even brief exposure to high oxygen concentrations during stabilization may trigger organ injury with adverse long-term outcomes in premature infants. We studied the long-term effects of short-term perinatal oxygen exposure on cell cycle gene expression and lung growth in adult mice. We randomized mice litters at birth to 21, 40, or 100%O2 for
-
Novel mechanisms underlying anti-polycystic ovary like syndrome effects of electroacupuncture in rats: suppressing SREBP1 to mitigate insulin resistance, mitochondrial dysfunction and oxidative stress Biol. Res. (IF 3.092) Pub Date : 2020-10-27 Yan Peng; Xinming Yang; Xi Luo; Chunhong Liu; Xia Cao; Hongyan Wang; Liyuan Guo
Acupuncture, a therapy of traditional Chinese medicine, is confirmed to exert the therapeutic action on polycystic ovary syndrome (PCOS). However, the detailed therapeutic mechanisms of acupuncture in PCOS remain ambiguous. In this study, we further investigated whether electroacupuncture (EA) alleviated PCOS-like symptoms in rats via regulating a metabolic regulator, sterol regulatory element binding
-
LncRNA OIP5-AS1 upregulates snail expression by sponging miR-34a to promote ovarian carcinoma cell invasion and migration Biol. Res. (IF 3.092) Pub Date : 2020-10-22 Xingzhi Jiang; Zhongxue Ye; Yafen Jiang; Wen Yu; Qian Fang
Although OIP5-AS1 has been characterized as an oncogenic lncRNA in many types of cancer, its role and underlying mechanism in ovarian carcinoma (OC) remains unknown. This study aimed to investigate the role of OIP5-AS1 in OC. OC tissues and non-tumor tissues (ovary tissues within 3 cm around tumors) were collected from 58 OC patients (age range 36 to 67 years old, mean age 51.4 ± 5.9 years old). The
-
Daphnetin inhibits proliferation and inflammatory response in human HaCaT keratinocytes and ameliorates imiquimod-induced psoriasis-like skin lesion in mice Biol. Res. (IF 3.092) Pub Date : 2020-10-20 Jintao Gao; Fangru Chen; Huanan Fang; Jing Mi; Qi Qi; Mengjuan Yang
Psoriasis is a common chronic inflammatory skin disease. Keratinocytes hyperproliferation and excessive inflammatory response contribute to psoriasis pathogenesis. The agents able to attenuate keratinocytes hyperproliferation and excessive inflammatory response are considered to be potentially useful for psoriasis treatment. Daphnetin exhibits broad bioactivities including anti-proliferation and anti-inflammatory
-
Fate of nitrogen in agriculture and environment: agronomic, eco-physiological and molecular approaches to improve nitrogen use efficiency Biol. Res. (IF 3.092) Pub Date : 2020-10-16 Muhammad Anas; Fen Liao; Krishan K. Verma; Muhammad Aqeel Sarwar; Aamir Mahmood; Zhong-Liang Chen; Qiang Li; Xu-Peng Zeng; Yang Liu; Yang-Rui Li
Nitrogen is the main limiting nutrient after carbon, hydrogen and oxygen for photosynthetic process, phyto-hormonal, proteomic changes and growth-development of plants to complete its lifecycle. Excessive and inefficient use of N fertilizer results in enhanced crop production costs and atmospheric pollution. Atmospheric nitrogen (71%) in the molecular form is not available for the plants. For world’s
-
Evaluating the clinical significance of SHMT2 and its co-expressed gene in human kidney cancer Biol. Res. (IF 3.092) Pub Date : 2020-10-16 Huan Wang; Tie Chong; Bo-Yong Li; Xiao-San Chen; Wen-Bo Zhen
Kidney cancer is one of the most common cancers in the world. It is necessary to clarify its underlying mechanism and find its prognostic biomarkers. Current studies showed that SHMT2 may be participated in several kinds of cancer. Our studies investigated the expression of SHMT2 in kidney cancer by Oncomine, Human Protein Atlas database and ULCAN database. Meanwhile, we found its co-expression gene
-
Increased glucose metabolism in Arid5b−/− skeletal muscle is associated with the down-regulation of TBC1 domain family member 1 (TBC1D1) Biol. Res. (IF 3.092) Pub Date : 2020-10-06 Yuri Okazaki; Jennifer Murray; Ali Ehsani; Jessica Clark; Robert H. Whitson; Lisa Hirose; Noriyuki Yanaka; Keiichi Itakura
Skeletal muscle has an important role in regulating whole-body energy homeostasis, and energy production depends on the efficient function of mitochondria. We demonstrated previously that AT-rich interactive domain 5b (Arid5b) knockout (Arid5b−/−) mice were lean and resistant to high-fat diet (HFD)-induced obesity. While a potential role of Arid5b in energy metabolism has been suggested in adipocytes
-
LncRNA HCG11 regulates proliferation and apoptosis of vascular smooth muscle cell through targeting miR-144-3p/FOXF1 axis in atherosclerosis Biol. Res. (IF 3.092) Pub Date : 2020-10-02 Yi Liu; Xiyun Cui; Cong Wang; Sihai Zhao
Atherosclerosis (AS) is the main pathological basis of coronary heart disease, cerebral infarction and peripheral vascular disease, which seriously endanger people’s life and health. In recent years, long non-coding RNA (lncRNA) has been found to be involved in gene expression regulation, but the research on AS is still in the initial stage. In this study, we mainly studied the role of HCG11 in patients
-
Inhibition of phospholipase D2 augments histone deacetylase inhibitor-induced cell death in breast cancer cells Biol. Res. (IF 3.092) Pub Date : 2020-10-01 Won Chan Hwang; Dong Woo Kang; Youra Kang; Younghoon Jang; Jung-Ae Kim; Do Sik Min
Histone deacetylase (HDAC) inhibitors are promising anticancer drugs but their effect on tumor treatment has been disappointing mainly due to the acquisition of HDAC inhibitor resistance. However, the mechanisms underlying such resistance remain unclear. In this study, we performed Western blot, q-PCR, and promoter assay to examine the expression of HDAC inhibitor-induced phospholipase D2 (PLD2) in
-
MiR-128 suppresses metastatic capacity by targeting metadherin in breast cancer cells Biol. Res. (IF 3.092) Pub Date : 2020-09-29 Danxia Cao; Han Zhu; Qian Zhao; Jianming Huang; Cixiang Zhou; Jianrong He; Yongjun Liang
Breast cancer, the most common cancer in women worldwide, causes the vast majority of cancer-related deaths. Undoubtedly, tumor metastasis and recurrence are responsible for more than 90 percent of these deaths. MicroRNAs are endogenous noncoding RNAs that have been integrated into almost all the physiological and pathological processes, including metastasis. In the present study, the role of miR-128
-
Tumor suppressive function of Matrin 3 in the basal-like breast cancer Biol. Res. (IF 3.092) Pub Date : 2020-09-25 Jaehyuk Yang; Seung Jun Lee; Yongseok Kwon; Li Ma; Jongchan Kim
Basal-like breast cancer (BLBC) or triple-negative breast cancer (TNBC) is an aggressive and highly metastatic subtype of human breast cancer. The present study aimed to elucidate the potential tumor-suppressive function of MATR3, an abundant nuclear protein, in BLBC/TNBC, whose cancer-relevance has not been characterized. We analyzed in vitro tumorigenecity by cell proliferation and soft agar colony
-
REC8 suppresses tumor angiogenesis by inhibition of NF-κB-mediated vascular endothelial growth factor expression in gastric cancer cells. Biol. Res. (IF 3.092) Pub Date : 2020-09-21 Miao Liu,Wanfu Xu,Mingmin Su,Pingsheng Fan
Tumor angiogenesis is an essential event for tumor growth and metastasis. It has been showed that REC8, a component of the meiotic cohesion complex, played a vital role in Epithelial-Mesenchymal Transition (EMT) in gastric cancer. However, the role of REC8 in gastric cancer angiogenesis remains to be identified. Inhibition of REC8 expression in gastric cancer cells contributed to tumor angiogenesis
-
The roles of miRNA, lncRNA and circRNA in the development of osteoporosis. Biol. Res. (IF 3.092) Pub Date : 2020-09-16 Yang Yang,Wang Yujiao,Wang Fang,Yuan Linhui,Guo Ziqi,Wei Zhichen,Wang Zirui,Wang Shengwang
Osteoporosis is a common metabolic bone disease, influenced by genetic and environmental factors, that increases bone fragility and fracture risk and, therefore, has a serious adverse effect on the quality of life of patients. However, epigenetic mechanisms involved in the development of osteoporosis remain unclear. There is accumulating evidence that epigenetic modifications may represent mechanisms
-
Palmatine ameliorates high fat diet induced impaired glucose tolerance. Biol. Res. (IF 3.092) Pub Date : 2020-09-14 Xusheng Tian,Yukun Zhang,Han Li,Yunfeng Li,Ning Wang,Wei Zhang,Boyan Ma
The impaired glucose tolerance (IGT) is a representative prediabetes characterized by defective glucose homeostasis, and palmatine (PAL) is a natural isoquinoline alkaloid with multiple pharmacological effects. Our study aims to investigate the therapeutic effect of PAL on the impaired glucose tolerance. Male Sprague–Dawley rats were used to establish an IGT model with high fat diet (HFD). Oral glucose
-
LncRNA SNHG14 promotes OGD/R-induced neuron injury by inducing excessive mitophagy via miR-182-5p/BINP3 axis in HT22 mouse hippocampal neuronal cells. Biol. Res. (IF 3.092) Pub Date : 2020-09-10 Zexiang Deng,Hao Ou,Fei Ren,Yujiao Guan,Ye Huan,Hongwei Cai,Bei Sun
Long non-coding RNA (lncRNA) small nucleolar RNA host gene 14 (SNHG14) is associated with cerebral ischemia–reperfusion (CI/R) injury. This work aims to explore the role of SNHG14 in CI/R injury. HT22 (mouse hippocampal neuronal cells) cell model was established by oxygen–glucose deprivation/reoxygenation (OGD/R) treatment. The interaction among SNHG14, miR-182-5p and BNIP3 was verified by luciferase
-
Induced in vitro adaptation for salt tolerance in date palm (Phoenix dactylifera L.) cultivar Khalas. Biol. Res. (IF 3.092) Pub Date : 2020-08-26 Suliman A Al-Khateeb,Abdullatif A Al-Khateeb,Muhammad N Sattar,Akbar S Mohmand
Soil salinity causes huge economic losses to agriculture productivity in arid and semiarid areas worldwide. The affected plants face disturbances in osmotic adjustment, nutrient transport, ionic toxicity and reduced photosynthesis. Conventional breeding approaches produce little success in combating various stresses in plants. However, non-conventional approaches, such as in vitro tissue culturing
-
Altered thalamic neurotransmitters metabolism and functional connectivity during the development of chronic constriction injury induced neuropathic pain. Biol. Res. (IF 3.092) Pub Date : 2020-08-26 Zhifu Wang,Sheng Huang,Xiangmei Yu,Long Li,Minguang Yang,Shengxiang Liang,Weilin Liu,Jing Tao
To investigate the thalamic neurotransmitters and functional connections in the development of chronic constriction injury (CCI)-induced neuropathic pain. The paw withdrawal threshold was measured by mechanical stimulation the right hind paw with the von frey hair in the rats of CCI-induced neuropathic pain. The N-acetylaspartate (NAA) and Glutamate (Glu) in thalamus were detected by magnetic resonance
-
Hypoxia inducible factor-1 (HIF-1α) reduced inflammation in spinal cord injury via miR-380-3p/ NLRP3 by Circ 0001723. Biol. Res. (IF 3.092) Pub Date : 2020-08-20 Xigong Li,Xianfeng Lou,Sanzhong Xu,Junhua Du,Junsong Wu
Spinal cord injury (SCI) is a severe central nervous system trauma. The present study aimed to evaluate the effect of HIF-1α on inflammation in spinal cord injury (SCI) to uncover the molecular mechanisms of anti-inflammation. HIF-1α was reduced in SCI model rats and HIF-1α activation reduced TNF-α, IL-1β, IL-6 and IL-18 levels in SCI model rats. Meanwhile, Circ 0001723 expression was down-regulated
-
β-Catenin: oncogenic role and therapeutic target in cervical cancer. Biol. Res. (IF 3.092) Pub Date : 2020-08-05 Bingqi Wang,Xianping Li,Lei Liu,Min Wang
Cervical cancer is a common and fatal malignancy of the female reproductive system. Human papillomavirus (HPV) is the primary causal agent for cervical cancer, but HPV infection alone is insufficient to cause the disease. Actually, most HPV infections are sub-clinical and cleared spontaneously by the host immune system; very few persist and eventually develop into cervical cancer. Therefore, other
-
Microarray profiling and functional analysis of differentially expressed plasma exosomal circular RNAs in Graves' disease. Biol. Res. (IF 3.092) Pub Date : 2020-07-29 Ying Sun,Wei Wang,Yuxiao Tang,Daping Wang,Liang Li,Min Na,Guantong Jiang,Qian Li,Shulin Chen,Jin Zhou
Circulating RNA (circRNA) regulates various bioactivities in cells. A better understanding of the exosomal circRNA can provide novel insights into the pathogenesis and treatment of Graves’ disease (GD). We aimed to profile the differentially expressed circRNAs (DEcRs) in plasma exosomes of patients with GD and speculate and probe the functions of the DEcR by comprehensive bioinformatics analyses. Serum
-
Curcumin prevents neuronal loss and structural changes in the superior cervical (sympathetic) ganglion induced by chronic sleep deprivation, in the rat model. Biol. Res. (IF 3.092) Pub Date : 2020-07-10 Mahboobeh Erfanizadeh,Ali Noorafshan,Mohammad Reza Namavar,Saied Karbalay-Doust,Tahereh Talaei-Khozani
In modern societies, sleep deprivation is a serious health problem. This problem could be induced by a variety of reasons, including lifestyle habits or neurological disorders. Chronic sleep deprivation (CSD) could have complex biological consequences, such as changes in neural autonomic control, increased oxidative stress, and inflammatory responses. The superior cervical ganglion (SCG) is an important
-
Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related species. Biol. Res. (IF 3.092) Pub Date : 2020-07-07 Lucas Fernandes Rocha,Natália Ribeiro Paula,Alison Gonçalves Nazareno,Dulcinéia de Carvalho
We developed simple sequence repeats (SSR) for Eremanthus erythropappus (DC.) MacLeish, an endangered tree species endemic to the Brazilian Savanna and Atlantic Forest biomes, and tested their transferability to two closely related Eremanthus species. Using a genomic library enriched with tandem repeat motifs, we identified 16 primer pairs, and characterized them in two populations. Nine primers amplified
-
Microbial communities from arid environments on a global scale. A systematic review. Biol. Res. (IF 3.092) Pub Date : 2020-07-06 Javiera Vásquez-Dean,Felipe Maza,Isidora Morel,Rodrigo Pulgar,Mauricio González
Arid environments are defined by the lack of water availability, which is directly related to the mean annual precipitation (MAP), and high values of solar irradiation, which impacts the community composition of animals, plants, and the microbial structure of the soil. Recent advances in NGS technologies have expanded our ability to characterize microbiomes, allowing environmental microbiologists to
-
Edaravone alleviates cell apoptosis and mitochondrial injury in ischemia-reperfusion-induced kidney injury via the JAK/STAT pathway. Biol. Res. (IF 3.092) Pub Date : 2020-07-03 Xiaoying Zhao,Erfei Zhang,Xiaofen Ren,Xiaoli Bai,Dongming Wang,Ling Bai,Danlei Luo,Zheng Guo,Qiang Wang,Jianxin Yang
Kidney ischemia–reperfusion injury is a common pathophysiological phenomenon in the clinic. A large number of studies have found that the tyrosine protein kinase/signal transducer and activator of transcription (JAK/STAT) pathway is involved in the development of a variety of kidney diseases and renal protection associated with multiple drugs. Edaravone (EDA) is an effective free radical scavenger
-
Silencing of circular RNA ANRIL attenuates oxygen-glucose deprivation and reoxygenation-induced injury in human brain microvascular endothelial cells by sponging miR-622. Biol. Res. (IF 3.092) Pub Date : 2020-07-02 Su Jiang,Gaonian Zhao,Jun Lu,Min Jiang,Zhenggang Wu,Yujing Huang,Jing Huang,Jinghua Shi,Jing Jin,Xinxuan Xu,Xuehua Pu
Circular RNA (circRNA) is highly expressed in the brain tissue, but its molecular mechanism in cerebral ischemia–reperfusion remains unclear. Here, we explored the role and underlying mechanisms of circRNA antisense non-coding RNA in the INK4 locus (circ_ANRIL) in oxygen–glucose deprivation and reoxygenation (OGD/R)-induced cell injury. The expression of circ_ANRIL in OGD/R-induced human brain microvascular
-
Understanding gold toxicity in aerobically-grown Escherichia coli. Biol. Res. (IF 3.092) Pub Date : 2020-06-08 C Muñoz-Villagrán,F Contreras,F Cornejo,M Figueroa,D Valenzuela-Bezanilla,R Luraschi,C Reinoso,J Rivas-Pardo,C Vásquez,M Castro,F Arenas
There is an emerging field to put into practice new strategies for developing molecules with antimicrobial properties. In this line, several metals and metalloids are currently being used for these purposes, although their cellular effect(s) or target(s) in a particular organism are still unknown. Here we aimed to investigate and analyze Au3+ toxicity through a combination of biochemical and molecular
-
Overexpression of bHLH domain of HIF-1 failed to inhibit the HIF-1 transcriptional activity in hypoxia. Biol. Res. (IF 3.092) Pub Date : 2020-06-05 Fatemeh Sadeghi,Gholam Ali Kardar,Mohammad Reza Bolouri,Farzad Nasri,Maryam Sadri,Reza Falak
Hypoxia inducible factor-1 (HIF-1) is considered as the most activated transcriptional factor in response to low oxygen level or hypoxia. HIF-1 binds the hypoxia response element (HRE) sequence in the promoter of different genes, mainly through the bHLH domain and activates the transcription of genes, especially those involved in angiogenesis and EMT. Considering the critical role of bHLH in binding
-
Eukaryotic expression, Co-IP and MS identify BMPR-1B protein-protein interaction network. Biol. Res. (IF 3.092) Pub Date : 2020-05-29 Jianlei Jia,Jipeng Jin,Qian Chen,Zan Yuan,Haiqin Li,Junhao Bian,Linsheng Gui
BMPR-1B is part of the transforming growth factor β super family and plays a pivotal role in ewe litter size. Functional loss of exon-8 mutations in the BMPR-1B gene (namely the FecB gene) can increase both the ewe ovulation rate and litter size. This study constructed a eukaryotic expression system, prepared a monoclonal antibody, and characterized BMPR-1B/FecB protein–protein interactions (PPIs)
-
Genome-wide characterization of the abscisic acid-, stress- and ripening-induced (ASR) gene family in wheat (Triticum aestivum L.). Biol. Res. (IF 3.092) Pub Date : 2020-05-24 Huawei Li,Haiying Guan,Qicui Zhuo,Zongshuai Wang,Shengdong Li,Jisheng Si,Bin Zhang,Bo Feng,Ling-An Kong,Fahong Wang,Zheng Wang,Lishun Zhang
BACKGROUND Abscisic acid-, stress-, and ripening-induced (ASR) genes are a class of plant specific transcription factors (TFs), which play important roles in plant development, growth and abiotic stress responses. The wheat ASRs have not been described in genome-wide yet. METHODS We predicted the transmembrane regions and subcellular localization using the TMHMM server, and Plant-mPLoc server and CELLO
-
Engraftment of human induced pluripotent stem cell-derived myogenic progenitors restores dystrophin in mice with duchenne muscular dystrophy. Biol. Res. (IF 3.092) Pub Date : 2020-05-19 Ruojie He,Huan Li,Liang Wang,Yaqin Li,Yu Zhang,Menglong Chen,Yuling Zhu,Cheng Zhang
BACKGROUND Duchenne muscular dystrophy (DMD) is a devastating genetic muscular disorder with no effective treatment that is caused by the loss of dystrophin. Human induced pluripotent stem cells (hiPSCs) offer a promising unlimited resource for cell-based therapies of muscular dystrophy. However, their clinical applications are hindered by inefficient myogenic differentiation, and moreover, the engraftment
-
Development of nuclear SSR and chloroplast genome markers in diverse Liriodendron chinense germplasm based on low-coverage whole genome sequencing. Biol. Res. (IF 3.092) Pub Date : 2020-05-14 Bin Li,Furong Lin,Ping Huang,Wenying Guo,Yongqi Zheng
BACKGROUND Liriodendron chinense ranges widely in subtropical China and northern Vietnam; however, it inhabits several small, isolated populations and is now an endangered species due to its limited seed production. The objective of this study was to develop a set of nuclear SSR (simple sequence repeats) and multiple chloroplast genome markers for genetic studies in L. chinense and their characterization
-
Analysis of the role of the interleukins in colon cancer. Biol. Res. (IF 3.092) Pub Date : 2020-05-07 Xiyi Wei,Yuan Zhang,Zhou Yang,Yeqin Sha,Yitong Pan,Yusheng Chen,Lei Cai
BACKGROUND The role of interleukin family in colon cancer remained controversial. The purpose of this study was to investigate the association between interleukin family and colon cancer progression through bioinformatics methods and to validate such association in clinical patients. METHODS A total of 15 differentially expressed interleukins between the colon cancer tissue and normal colon tissue
-
miR-185-5p response to usnic acid suppresses proliferation and regulating apoptosis in breast cancer cell by targeting Bcl2. Biol. Res. (IF 3.092) Pub Date : 2020-05-04 Elif Değerli,Vildan Torun,Demet Cansaran-Duman
BACKGROUND Breast cancer is the most common cancer types among women. Recent researches have focused on determining the efficiency of alternative molecules and miRNAs in breast cancer treatment. The aim of this study was to determine the effect of usnic acid response-miR-185-5p on proliferation in the breast cancer cell and to determine its relationship with apoptosis pathway. METHODS The cell proliferation
-
Knockdown of long non-coding RNA HOTAIR reverses cisplatin resistance of ovarian cancer cells through inhibiting miR-138-5p-regulated EZH2 and SIRT1. Biol. Res. (IF 3.092) Pub Date : 2020-04-29 Yun Zhang,Hao Ai,Xue Fan,Suxian Chen,Yadi Wang,Lili Liu
BACKGROUND Cisplatin resistance (DDP-resistance) remains one of the major causes of poor prognosis in females with ovarian cancer. Long non-coding RNAs (lncRNAs) have been shown to participate in the regulation of cellular processes, including chemoresistance. The aim of this study was to explore the role of HOX transcript antisense RNA (HOTAIR) in DDP-resistant ovarian cancer cells. METHODS DDP-resistant
-
Inhibition of miR-19a-3p decreases cerebral ischemia/reperfusion injury by targeting IGFBP3 in vivo and in vitro. Biol. Res. (IF 3.092) Pub Date : 2020-04-20 Zhaohui Chai,Jiangbiao Gong,Peidong Zheng,Jiesheng Zheng
BACKGROUND Inflammation and apoptosis are considered to be two main factors affecting ischemic brain injury and the subsequent reperfusion damage. MiR-19a-3p has been reported to be a possible novel biomarker in ischemic stroke. However, the function and molecular mechanisms of miR-19a-3p remain unclear in cerebral ischemia/reperfusion (I/R) injury. METHODS The I/R injury model was established in vivo
-
Correction to: Identification of the targets of hematoporphyrin derivative in lung adenocarcinoma using integrated network analysis. Biol. Res. (IF 3.092) Pub Date : 2020-04-19 Hongtao Yin,Yan Yu
The Fig. 1b is wrongly published in the original publication of the article. The correct version of Fig. 1b is presented in this Correction.
-
Development of a small panel of SNPs to infer ancestry in Chileans that distinguishes Aymara and Mapuche components. Biol. Res. (IF 3.092) Pub Date : 2020-04-16 Ricardo A Verdugo,Alex Di Genova,Luisa Herrera,Mauricio Moraga,Mónica Acuña,Soledad Berríos,Elena Llop,Carlos Y Valenzuela,M Leonor Bustamante,Dayhana Digman,Adriana Symon,Soledad Asenjo,Pamela López,Alejandro Blanco,José Suazo,Emmanuelle Barozet,Fresia Caba,Marcelo Villalón,Sergio Alvarado,Dante Cáceres,Katherine Salgado,Pilar Portales,Andrés Moreno-Estrada,Christopher R Gignoux,Karla Sandoval,Carlos
BACKGROUND Current South American populations trace their origins mainly to three continental ancestries, i.e. European, Amerindian and African. Individual variation in relative proportions of each of these ancestries may be confounded with socio-economic factors due to population stratification. Therefore, ancestry is a potential confounder variable that should be considered in epidemiologic studies
-
LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p. Biol. Res. (IF 3.092) Pub Date : 2020-04-15 Min Luo,Chengbai Liang
BACKGROUND Previous studies have shown that long noncoding RNA (lncRNA) LINC00483 was aberrantly expressed in human cancers, including gastric cancer. However, the regulatory mechanism of this lncRNA in gastric cancer remains largely unknown. The present study aimed to investigate the effect of LINC00483 on gastric cancer development and explore the potential regulatory network of LINC00483/microRNA
-
Functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor. Biol. Res. (IF 3.092) Pub Date : 2020-04-15 Patricia García,Carolina Bizama,Lorena Rosa,Jaime A Espinoza,Helga Weber,Javier Cerda-Infante,Marianela Sánchez,Viviana P Montecinos,Justo Lorenzo-Bermejo,Felix Boekstegers,Marcela Dávila-López,Francisca Alfaro,Claudia Leiva-Acevedo,Zasha Parra,Diego Romero,Sumie Kato,Pamela Leal,Marcela Lagos,Juan Carlos Roa
BACKGROUND Gallbladder cancer (GBC) is the most common tumor of the biliary tract. The incidence of GBC shows a large geographic variability, being particularly frequent in Native American populations. In Chile, GBC represents the second cause of cancer-related death among women. We describe here the establishment of three novel cell lines derived from the ascitic fluid of a Chilean GBC patient, who
-
Mast cells-derived MiR-223 destroys intestinal barrier function by inhibition of CLDN8 expression in intestinal epithelial cells. Biol. Res. (IF 3.092) Pub Date : 2020-03-24 Musheng Li,Junhong Zhao,Meiwan Cao,Ruitao Liu,Guanhua Chen,Songyu Li,Yuanwen Xie,Jing Xie,Yang Cheng,Ling Huang,Mingmin Su,Yuxin Xu,Mingyue Zheng,Kejian Zou,Lanlan Geng,Wanfu Xu,Sitang Gong
BACKGROUND Mast cells (MCs) have been found to play a critical role during development of inflammatory bowel disease (IBD) that characterized by dysregulation of inflammation and impaired intestinal barrier function. However, the function of MCs in IBD remains to be fully elucidated. RESULTS In our study, we used exosomes isolated from human mast cells-1 (HMCs-1) to culture with NCM460, HT-29 or CaCO2
-
MicroRNA-107 is a novel tumor suppressor targeting POU3F2 in melanoma Biol. Res. (IF 3.092) Pub Date : 2020-03-14 Guizhi Zhao; Zhili Wei; Yang Guo
Melanoma is one of the major types of skin cancer. The metastatic melanoma is among the most lethal forms of malignant skin tumors. We hereby aimed to characterize a novel microRNA (miR) in the metastatic melanoma model. First, we evaluated the expression of miR-107 in melanoma cells and tumor tissues. The comparison between primary and metastatic cancer tissues was also accessed. Next, we examined
-
Mir-30b-3p affects the migration and invasion function of ovarian cancer cells by targeting the CTHRC1 gene Biol. Res. (IF 3.092) Pub Date : 2020-03-10 Yan Li; Jinhua Zhou; Juan Wang; Xiaoping Chen; Yan Zhu; Youguo Chen
The aim of this study was to investigate the effect role and mechanism of miR-30b-3p on ovarian cancer cells biological function. The expression of miR-30b-3p was detected in ovarian cancer cell lines and normal ovarian epithelial cell line by qRT-PCR. Mir-30b-3p mimic was transfected into OVCAR3 cells. Cell-counting kit-8 (CCK-8) assay was conducted to explore the effect of mir-30b-3p on the OVCAR3
-
Studies on the cold tolerance of ratoon ‘Chaling’ common wild rice Biol. Res. (IF 3.092) Pub Date : 2020-02-18 Mengliang Xu; Xiangzhen Li; Xiang Mo; Siyu Tu; Yanchun Cui; Daichang Yang
Rice is the staple food of many people around the world. However, most rice varieties, especially widely grown indica varieties and hybrids, are sensitive to cold stress. In order to provide a basis for the utilization of a common wild rice (CWR, Oryza rufipogon Griff.) named ‘Chaling’ CWR in cold-tolerant rice breeding and deepen the understanding of rice cold tolerance, the cold tolerance of ratoon
-
LncRNA SNHG7/miR-34a-5p/SYVN1 axis plays a vital role in proliferation, apoptosis and autophagy in osteoarthritis Biol. Res. (IF 3.092) Pub Date : 2020-02-17 Feng Tian; Junhu Wang; Zhanhua Zhang; Jie Yang
Osteoarthritis (OA) is one of the most common rheumatic diseases of which clinical symptoms includes swelling, synovitis and inflammatory pain, affect patients’ daily life. It was reported that non-coding RNAs play vital roles in OA. However, the regulation mechanism of ncRNA in OA pathogenesis has not been fully elucidated. The expression of SNHG7, miR-34a-5p and SYVN1 was detected using qRT-PCR in
-
CtpB is a plasma membrane copper (I) transporting P-type ATPase of Mycobacterium tuberculosis. Biol. Res. (IF 3.092) Pub Date : 2020-02-13 Andrés León-Torres,Epifania Arango,Eliana Castillo,Carlos Y Soto
BACKGROUND The intracellular concentration of heavy-metal cations, such as copper, nickel, and zinc is pivotal for the mycobacterial response to the hostile environment inside macrophages. To date, copper transport mediated by P-type ATPases across the mycobacterial plasma membrane has not been sufficiently explored. RESULTS In this work, the ATPase activity of the putative Mycobacterium tuberculosis
-
Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor microenvironment. Biol. Res. (IF 3.092) Pub Date : 2020-02-11 Qing Zhu,Jingchao Li,Qi Wu,Yongxia Cheng,Huizhe Zheng,Tao Zhan,Hongwei Wang,Yue Yang,Hongyan Wang,Ye Liu,Sufen Guo
BACKGROUND LincRNAs have been revealed to be tightly associated with various tumorigeneses and cancer development, but the roles of specific lincRNA on tumor-related angiogenesis was hardly studied. Here, we aimed to investigate whether linc-OIP5 in breast cancer cells affects the angiogenesis of HUVECs and whether the linc-OIP5 regulations are involved in angiogenesis-related Notch and Hippo signaling
-
The comprehensive detection of miRNA, lncRNA, and circRNA in regulation of mouse melanocyte and skin development. Biol. Res. (IF 3.092) Pub Date : 2020-02-03 Zhiwei Zhu,Yueyue Ma,Yuan Li,Pengfei Li,Zhixue Cheng,Huifeng Li,Lihuan Zhang,Zhongwei Tang
BACKGROUND Pigmentation development, is a complex process regulated by many transcription factors during development. With the development of the RNA sequencing (RNA-seq), non-coding RNAs, such as miRNAs, lncRNAs, and circRNAs, are found to play an important role in the function detection of related regulation factors. In this study, we provided the expression profiles and development of ncRNAs related
-
Comparison of protein profiles of the pellicle, gingival crevicular fluid, and saliva: possible origin of pellicle proteins. Biol. Res. (IF 3.092) Pub Date : 2020-01-15 Hibiki Odanaka,Takashi Obama,Naoko Sawada,Marika Sugano,Hiroyuki Itabe,Matsuo Yamamoto
BACKGROUND The pellicle, the acellular organic material deposited on the surface of tooth enamel, has been thought to be derived from saliva. In this study, protein compositions of the pellicle, gingival crevicular fluid, and saliva collected from healthy adults were compared to elucidate the origin of pellicle proteins. RESULTS The pellicle, gingival crevicular fluid, and saliva from the parotid gland
-
Disentangling the genetic bases of Saccharomyces cerevisiae nitrogen consumption and adaptation to low nitrogen environments in wine fermentation Biol. Res. (IF 3.092) Pub Date : 2020-01-09 Eduardo I. Kessi-Pérez; Jennifer Molinet; Claudio Martínez
The budding yeast Saccharomyces cerevisiae has been considered for more than 20 years as a premier model organism for biological sciences, also being the main microorganism used in wide industrial applications, like alcoholic fermentation in the winemaking process. Grape juice is a challenging environment for S. cerevisiae, with nitrogen deficiencies impairing fermentation rate and yeast biomass production
-
SNHG1 promotes MPP+-induced cytotoxicity by regulating PTEN/AKT/mTOR signaling pathway in SH-SY5Y cells via sponging miR-153-3p Biol. Res. (IF 3.092) Pub Date : 2020-01-06 Jun Zhao; Lijiao Geng; Yong Chen; Chunfang Wu
Long non-coding RNA small molecule RNA host gene 1 (SNHG1) was previously identified to be relevant with Parkinson’s disease (PD) pathogenesis. This work aims to further elucidate the regulatory networks of SNHG1 involved in PD. 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-hydrochloride (MPTP)-induced mice and 1-methyl-4-phenylpyridinium (MPP+)-treated SH-SY5Y cells were respectively constructed as
-
LncRNA LINC00460 promotes the papillary thyroid cancer progression by regulating the LINC00460/miR-485-5p/Raf1 axis Biol. Res. (IF 3.092) Pub Date : 2019-12-23 Guangjun Li; Qingli Kong
Papillary thyroid cancer (PTC) is the most common malignancy of all thyroid cancers. LncRNA LINC00460 has been proved to play roles in the oncogenesis and progression of various tumors, including papillary thyroid cancer. However, the potential molecular mechanism of LINC00460 in PTC is poorly investigated. LINC00460 was upregulated in PTC tissues and cells. Raf1 was upregulated in PTC tissues, but
-
RASAL1 induces to downregulate the SCD1, leading to suppression of cell proliferation in colon cancer via LXRα/SREBP1c pathway Biol. Res. (IF 3.092) Pub Date : 2019-12-17 Guangchuan Wang; Zhen Li; Xiao Li; Chunqing Zhang; Lipan Peng
Recent studies have confirmed that RASAL1 has an antitumor effect in many cancers, but its functional role and the molecular mechanism underlying in colon cancer has not been investigated. We collected human colon cancer tissues and adjacent non-tumor tissues, human colon cancer cell lines LoVo, CaCo2, SW1116, SW480 and HCT-116, and normal colonic mucosa cell line NCM460. RT-qPCR was used to detect