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Genomic characterization of extended‐spectrum beta‐lactamase‐producing and carbapenem‐resistant Escherichia coli from urban wastewater in Australia Microbiologyopen (IF 3.4) Pub Date : 2024-03-16 Zillur Rahman, Mary‐Louise McLaws, Torsten Thomas
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Effects of color variation and physiological state on ascidian microbiomes Microbiologyopen (IF 3.4) Pub Date : 2024-03-14 Samantha K. Morrison, Patrick M. Erwin, Susanna López‐Legentil
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Complete nucleotide sequence and comparative genomic analysis of microcin B17 plasmid pMccB17 Microbiologyopen (IF 3.4) Pub Date : 2024-03-05 Mayokun Ajeigbe, Stephen Childs, Timothy A. Paget, Lewis E. H. Bingle
We present a comprehensive sequence and bioinformatic analysis of the prototypical microcin plasmid, pMccb17, which includes a definitive sequence for the microcin operon, mcb. Microcin B17 (MccB17) is a ribosomally synthesized and posttranslationally modified peptide produced by Escherichia coli. It inhibits bacterial DNA gyrase similarly to quinolone antibiotics. The mcb operon, which consists of
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Multidrug‐resistant Enterococcus faecium strains enter the Norwegian marine environment through treated sewage Microbiologyopen (IF 3.4) Pub Date : 2024-03-05 Vera Radisic, Didrik H. Grevskott, Nadja Junghardt, Lise Øvreås, Nachiket P. Marathe
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The copy number of the eukaryotic rRNA gene can be counted comprehensively Microbiologyopen (IF 3.4) Pub Date : 2024-03-04 Akinori Yabuki, Tatsuhiko Hoshino, Tamiko Nakamura, Keiko Mizuno
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Ultrastructural and glycoproteomic characterization of Prevotella intermedia: Insights into O‐glycosylation and outer membrane vesicles Microbiologyopen (IF 3.4) Pub Date : 2024-02-27 Xi Ye, Bindusmita Paul, Joyce Mo, Eric C. Reynolds, Debnath Ghosal, Paul D. Veith
Prevotella intermedia, a Gram‐negative bacterium from the Bacteroidota phylum, is associated with periodontitis. Other species within this phylum are known to possess the general O‐glycosylation system. The O‐glycoproteome has been characterized in several species, including Tannerella forsythia, Porphyromonas gingivalis, and Flavobacterium johnsoniae. In our study, we used electron cryotomography
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Effect of the polysaccharide capsule and its heptose on the resistance of Campylobacter jejuni to innate immune defenses Microbiologyopen (IF 3.4) Pub Date : 2024-02-20 Matthew Myles, Heba Barnawi, Mahmoud Mahmoudpour, Sargon Shlimon, Adrienne Chang, Daniel Zimmermann, Chiwon Choi, Najwa Zebian, Carole Creuzenet
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Characterizing living ocular bacterial communities and the effects of antibiotic perturbation in house finches Microbiologyopen (IF 3.4) Pub Date : 2024-02-04 Chava L. Weitzman, Dana M. Hawley, Bahman Rostama, Meghan May, Lisa K. Belden
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Genomic analyses of an Escherichia coli and Klebsiella pneumoniae urinary tract co-infection using long-read nanopore sequencing Microbiologyopen (IF 3.4) Pub Date : 2024-01-17 Stephen Mark Edward Fordham, Magdalena Barrow, Anna Mantzouratou, Elizabeth Sheridan
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Group A Streptococcus strains causing meningitis without distinct invasive phenotype Microbiologyopen (IF 3.4) Pub Date : 2024-01-04 Laura Marquardt, Federica Andreoni, Mathilde Boumasmoud, Tiziano A. Schweizer, Dorothea M. Heuberger, Elena Parietti, Sanne Hertegonne, Jana Epprecht, Dario Mattle, Anna K. Raez, Ewerton Marques-Maggio, Reto A. Schuepbach, Barbara Hasse, Srikanth Mairpady-Shambat, Silvio D. Brugger, Annelies S. Zinkernagel
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Serratia-based toxin cluster elements are associated with a type I fimbria Microbiologyopen (IF 3.4) Pub Date : 2024-01-02 Lesley Sitter, Marion Schoof, Travis R. Glare, Murray P. Cox, Peter C. Fineran, Paul P. Gardner, Mark R. H. Hurst
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Gut microbiota in the short-beaked echidna (Tachyglossus Aculeatus) shows stability across gestation Microbiologyopen (IF 3.4) Pub Date : 2023-12-14 Isini Buthgamuwa, Jane C. Fenelon, Alice Roser, Haley Meer, Stephen D. Johnston, Ashley M. Dungan
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Examining the functional space of gut microbiome-derived peptides Microbiologyopen (IF 3.4) Pub Date : 2023-12-12 Ying-Chiang J. Lee
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Bacillus-based probiotic cleansers reduce the formation of dry biofilms on common hospital surfaces Microbiologyopen (IF 3.4) Pub Date : 2023-11-22 Richard Wormald, Paul N. Humphreys, Christopher J. Charles, Simon P. Rout
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Correction to Concomitant knockout of target and transporter genes in filamentous fungi by genome coediting Microbiologyopen (IF 3.4) Pub Date : 2023-11-08
Tamano, K. (2022). Concomitant knockout of target and transporter genes in filamentous fungi by genome coediting. MicrobiologyOpen, e1280. https://doi.org/10.1002/mbo3.1280 In the above article, the words “autotrophy” and “autotrophic” should be read as “prototrophy” and “prototrophic,” respectively in all instances.
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Comparative genomic and metabolomic study of three Streptomyces sp. differing in biological activity Microbiologyopen (IF 3.4) Pub Date : 2023-10-30 Alisson Gillon, Ola Abdelrahman, Eliane Abou-Mansour, Floriane L'Haridon, Laurent Falquet, Pierre-Marie Allard, Laure Weisskopf
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Biosynthetic gene cluster synteny: Orthologous polyketide synthases in Hypogymnia physodes, Hypogymnia tubulosa, and Parmelia sulcata Microbiologyopen (IF 3.4) Pub Date : 2023-10-17 Nadim Ahmad, Manfred Ritz, Anjuli Calchera, Jürgen Otte, Imke Schmitt, Thomas Brueck, Norbert Mehlmer
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Isolation, characterization, and fibroblast uptake of bacterial extracellular vesicles from Porphyromonas gingivalis strains Microbiologyopen (IF 3.4) Pub Date : 2023-10-16 Helene R. Haugsten, Anne K. Kristoffersen, Trude M. Haug, Tine M. Søland, Reidun Øvstebø, Hans C. D. Aass, Morten Enersen, Hilde K. Galtung
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Transcriptomics-guided identification of an algicidal protease of the marine bacterium Kordia algicida OT-1 Microbiologyopen (IF 3.4) Pub Date : 2023-10-10 Kristy S. Syhapanha, David A. Russo, Yun Deng, Nils Meyer, Remington X. Poulin, Georg Pohnert
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Relationship between the Rod complex and peptidoglycan structure in Escherichia coli Microbiologyopen (IF 3.4) Pub Date : 2023-10-09 Risa Ago, Yuhei O. Tahara, Honoka Yamaguchi, Motoya Saito, Wakana Ito, Kaito Yamasaki, Taishi Kasai, Sho Okamoto, Taiki Chikada, Taku Oshima, Issey Osaka, Makoto Miyata, Hironori Niki, Daisuke Shiomi
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Dynamic interactions between Candida albicans and different streptococcal species in a multispecies oral biofilm Microbiologyopen (IF 3.4) Pub Date : 2023-10-03 Tenzin Kunchok Lueyar, Lamprini Karygianni, Thomas Attin, Thomas Thurnheer
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A loophole in soap dispensers mediates contamination with Gram-negative bacteria Microbiologyopen (IF 3.4) Pub Date : 2023-09-29 Ralf Lucassen, Nicole van Leuven, Dirk Bockmühl
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Similarity in milk microbiota in replicates Microbiologyopen (IF 3.4) Pub Date : 2023-09-30 Josef Dahlberg, Erik Pelve, Johan Dicksved
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Influence of light conditions on the production of chrysolaminarin using Phaeodactylum tricornutum in artificially illuminated photobioreactors Microbiologyopen (IF 3.4) Pub Date : 2023-09-18 Konstantin Frick, Tobias Ebbing, Yen-Cheng Yeh, Ulrike Schmid-Staiger, Günter E. M. Tovar
Here we examine the influence of the amount of light per gram biomass (specific light availability) and the influence of two different biomass densities (at the same amount of light per gram biomass) on the accumulation of the storage product chrysolaminarin and potential co-products: fucoxanthin, eicosapentaenoic acid, and fatty acids used for energy storage (C16 fatty acids), during nitrogen depletion
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Rifampicin-resistant RpoB S522L Vibrio vulnificus exhibits disturbed stress response and hypervirulence traits Microbiologyopen (IF 3.4) Pub Date : 2023-09-10 Laura Cutugno, Conor O'Byrne, Jan Pané-Farré, Aoife Boyd
Variant rpoB alleles affect Vibrio vulnificus stress responses and cytotoxicity. RpoB S522L mutants have decreased hypo-osmolarity tolerance and survival. Rifampicin-resistant RpoB S522L Vibrio vulnificus has a hypervirulent phenotype.
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Orientia tsutsugamushi: A life between escapes Microbiologyopen (IF 3.4) Pub Date : 2023-09-10 Lea Fromm, Jonas Mehl, Christian Keller
The life cycle of the mite-borne, obligate intracellular pathogen Orientia tsutsugamushi (Ot), the causative agent of human scrub typhus, differs in many aspects from that of other members of the Rickettsiales order. Particularly, the nonlytic cellular exit of individual Ot bacteria at the plasma membrane closely resembles the budding of enveloped viruses but has only been rudimentarily studied at
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Bacterial surface-exposed lipoproteins and sortase-mediated anchored cell surface proteins in plant infection Microbiologyopen (IF 3.4) Pub Date : 2023-09-14 Andrés de Sandozequi, Claudia Martínez-Anaya
We reviewed the literature on bacterial cell surface lipoproteins and proteins attached to the peptidoglycan layer and their importance in plant-bacteria interactions.
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Evaluating models and assessment techniques for understanding oral biofilm complexity Microbiologyopen (IF 3.4) Pub Date : 2023-08-29 Srinivas Sulugodu Ramachandra, Patricia Wright, Pingping Han, Abdalla Abdal-hay, Ryan S. B. Lee, Saso Ivanovski
This review focuses on diverse biofilm models and assessment strategies, highlighting the transition in the field from two-dimensional models to the recently explored novel three-dimensional models that aim to mimic biofilm development in the microenvironment of biological structures. The review further explores culture-dependent biofilm assessment strategies such as colony-forming unit assay, crystal
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Antibiofilm assay for antimicrobial peptides combating the sulfate-reducing bacteria Desulfovibrio vulgaris Microbiologyopen (IF 3.4) Pub Date : 2023-08-21 Lena Stillger, Lucile Viau, Dirk Holtmann, Daniela Müller
This study aimed to develop a fast and realistic antibiofilm assay for screening antimicrobial peptides (AMPs) for biocorrosive bacteria. Effective AMPs for both inhibition and removal of the biofilm could be identified and long-term inhibition of biofilm was possible for up to 21 days.
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Transforming the untransformable with knockout minicircles Microbiologyopen (IF 3.4) Pub Date : 2023-08-21 Oleksandra Rudenko, Laura Baseggio, Fynn McGuigan, Andrew C. Barnes
We describe efficient vector-free allelic exchange mutagenesis in Photobacterium damselae subsp. piscicida, potentially adaptable to any other bacterial species. It involves electroporation of minimalistic constructs containing only homology arms and selection markers, knockout minicircles, into cells rendered competent using concentrated sucrose solution. This approach is appropriate for genetic targets
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Cattle–compost–soil: The transfer of antibiotic resistance in livestock agriculture Microbiologyopen (IF 3.4) Pub Date : 2023-08-18 Fadhel Abbas, Phil Thomas, Bianca Cully-Duse, Nicholas M. Andronicos, Gal Winter
The study describes the analysis of antibiotic-resistant bacteria (ARB) abundance and the overall bacterial diversity of cattle farm soils that have been treated with animal manure compost. Our results showed that ARB abundance was greatest in fresh manure and significantly lower in composted manure. However, the application of composted manure on paddock soil led to a significant increase in soil
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Isolation and characterization of filamentous fungi capable of degrading the mycotoxin patulin Microbiologyopen (IF 3.4) Pub Date : 2023-08-11 Megumi Mita, Rina Sato, Miho Kakinuma, Hiroyuki Nakagawa, Toshiki Furuya
Filamentous fungi capable of degrading patulin were isolated. Acremonium sp. TUS-MM1 transforms patulin into less-toxic molecules by secreting a highly reactive compound. In addition, once patulin enters the cells, strain TUS-MM1 can transform it into desoxypatulinic acid to reduce its toxicity.
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Isolation, biochemical characterization, and genome sequencing of two high-quality genomes of a novel chitinolytic Jeongeupia species Microbiologyopen (IF 3.4) Pub Date : 2023-08-06 Nathanael D. Arnold, Daniel Garbe, Thomas B. Brück
In this study, a novel chitinolytic Jeongeupia species “wiesaeckerbachi” was isolated from soil samples, characterized biochemically, and sequenced with the long-read platform PacBio Sequel IIe. In silico analysis unraveled genomic differences to the closest related type strain Jeongeupia naejangsanensis TAS4-2 in addition to an usually extensive chitinolytic machinery.
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Co-localization of clinically relevant antibiotic- and heavy metal resistance genes on plasmids in Klebsiella pneumoniae from marine bivalves Microbiologyopen (IF 3.4) Pub Date : 2023-07-19 Fredrik Håkonsholm, Marit A. K. Hetland, Iren H. Löhr, Bjørn Tore Lunestad, Nachiket P. Marathe
Klebsiella pneumoniae is an opportunistic pathogen frequently associated with antibiotic resistance and present in a wide range of environments. In this study, five antibiotic-resistant K. pneumoniae isolates recovered from bivalves, of which four also carried heavy metal resistance genes, were selected for complete genome sequencing. This study shows the co-occurrence of antibiotic- and heavy metal
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Isolation and functional analysis of phage-displayed antibody fragments targeting the staphylococcal superantigen-like proteins Microbiologyopen (IF 3.4) Pub Date : 2023-07-16 Ida Alanko, Rebecca Sandberg, Eeva-Christine Brockmann, Carla J. C. de Haas, Jos A. G. van Strijp, Urpo Lamminmäki, Outi M. H. Salo-Ahen
In this study, we report the identification of phage-displayed scFv-antibodies able to recognize the staphylococcal superantigen-like proteins SS1, SSL5, and SSL10. All three SSLs bind to and inhibit host immune components and contribute to Staphylococcus aureus immune evasion. We were able to show in vitro that one scFv was able to inhibit SSL1 and maintain matrix metallopeptidase (MMP9) activity
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Clay-associated microbial communities and their relevance for a nuclear waste repository in the Opalinus Clay rock formation Microbiologyopen (IF 3.4) Pub Date : 2023-07-10 Julia Mitzscherling, Steffi Genderjahn, Anja M. Schleicher, Alexander Bartholomäus, Jens Kallmeyer, Dirk Wagner
Biocorrosion and microbial mineral transformation can affect the safety of deep geological repositories for high-level nuclear waste storage. We analyzed the composition and evaluated the importance of mineral-associated microbial communities in Opalinus Clay for safety purposes. Rock-attached communities were dominated by phyla capable of corrosive biofilm formation. Based on the abundance of potential
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Morphological and physiological impacts of salinity on colonial strains of the cyanobacteria Microcystis aeruginosa Microbiologyopen (IF 3.4) Pub Date : 2023-06-28 Myriam Bormans, Benjamin Legrand, Nicolas Waisbord, Enora Briand
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An improved method for intracellular DNA (iDNA) recovery from terrestrial environments Microbiologyopen (IF 3.4) Pub Date : 2023-06-25 Diego Medina Caro, Lucas Horstmann, Lars Ganzert, Romulo Oses, Thomas Friedl, Dirk Wagner
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Aedes albopictus microbiome derives from environmental sources and partitions across distinct host tissues Microbiologyopen (IF 3.4) Pub Date : 2023-06-12 Priscilla S. Seabourn, Danya E. Weber, Helen Spafford, Matthew C. I. Medeiros
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Genome comparison reveals that Halobacterium salinarum 63-R2 is the origin of the twin laboratory strains NRC-1 and R1 Microbiologyopen (IF 3.4) Pub Date : 2023-06-13 Friedhelm Pfeiffer, Mike Dyall-Smith
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Potential impacts of environmental bacteria on the microbiota of loggerhead (Caretta caretta) and green (Chelonia mydas) sea turtle eggs and their hatching success Microbiologyopen (IF 3.4) Pub Date : 2023-06-05 Colleen M. McMaken, Derek A. Burkholder, Rosanna J. Milligan, Jose V. Lopez
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Molecular mechanisms of regulation by a β-alanine-responsive Lrp-type transcription factor from Acidianus hospitalis Microbiologyopen (IF 3.4) Pub Date : 2023-05-22 Amber J. Bernauw, Vincent Crabbe, Fraukje Ryssegem, Ronnie Willaert, Indra Bervoets, Eveline Peeters
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Development of a new multiplex quantitative PCR for the detection of Glaesserella parasuis, Mycoplasma hyorhinis, and Mycoplasma hyosynoviae Microbiologyopen (IF 3.4) Pub Date : 2023-05-15 Simone Scherrer, Sarah Schmitt, Fenja Rademacher, Peter Kuhnert, Giovanni Ghielmetti, Sophie Peterhans, Roger Stephan
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Effects of sponge-to-sponge contact on the microbiomes of three spatially competing Caribbean coral reef species Microbiologyopen (IF 3.4) Pub Date : 2023-04-27 Shelby E. Gantt, Patrick M. Erwin
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Responses of Anabaena sp. PCC7120 to lindane: Physiological effects and differential expression of potential lin genes Microbiologyopen (IF 3.4) Pub Date : 2023-04-27 Jorge Guío, Maria F. Fillat, Maria L. Peleato, Emma Sevilla
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Genomics of the “tumorigenes” clade of the family Rhizobiaceae and description of Rhizobium rhododendri sp. nov Microbiologyopen (IF 3.4) Pub Date : 2023-03-31 Nemanja Kuzmanović, George C. diCenzo, Boyke Bunk, Cathrin Spröer, Anja Frühling, Meina Neumann-Schaal, Jörg Overmann, Kornelia Smalla
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High prevalence of blaCTX-M-15 type extended-spectrum beta-lactamases in Gambian hooded vultures (Necrosyrtes monachus): A threatened species with substantial human interaction Microbiologyopen (IF 3.4) Pub Date : 2023-03-23 Hanna Woksepp, Fagimba Camara, Jonas Bonnedahl
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Biological and synthetic surfactant exposure increases antimicrobial gene occurrence in a freshwater mixed microbial biofilm environment Microbiologyopen (IF 3.4) Pub Date : 2023-03-17 Stephanie P. Gill, William J. Snelling, James S. G. Dooley, Nigel G. Ternan, Ibrahim M. Banat, Joerg Arnscheidt, William R. Hunter
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Temporal disturbance of a model stream ecosystem by high microbial diversity from treated wastewater Microbiologyopen (IF 3.4) Pub Date : 2023-03-14 Tom L. Stach, Guido Sieber, Manan Shah, Sophie A. Simon, André Soares, Till L. V. Bornemann, Julia Plewka, Julian Künkel, Christian Becker, Folker Meyer, Jens Boenigk, Alexander J. Probst
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A parasitic nematode induces dysbiosis in susceptible but not resistant gastropod hosts Microbiologyopen (IF 3.4) Pub Date : 2023-03-10 Laura Sheehy, Kerry MacDonald-Howard, Chris D. Williams, Gareth D. Weedall, Hayley Jones, Robbie Rae
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Highly efficient export of a disulfide-bonded protein to the periplasm and medium by the Tat pathway using CyDisCo in Escherichia coli Microbiologyopen (IF 3.4) Pub Date : 2023-03-08 Klaudia Arauzo-Aguilera, Mirva J. Saaranen, Colin Robinson, Lloyd W. Ruddock
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The abundance of the potential pathogen Staphylococcus hominis in the air microbiome in a dental clinic and its susceptibility to far-UVC light Microbiologyopen (IF 3.4) Pub Date : 2023-03-05 Marilena Aquino de Muro, Igor Shuryak, Anne-Catrin Uhlemann, Alice Tillman, Dwayne Seeram, Joseph Zakaria, David Welch, Steven M. Erde, David J. Brenner
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Bioinformatic survey of CRISPR loci across 15 Serratia species Microbiologyopen (IF 3.4) Pub Date : 2023-03-02 Maria Scrascia, Roberta Roberto, Pietro D'Addabbo, Yosra Ahmed, Francesco Porcelli, Marta Oliva, Carla Calia, Angelo Marzella, Carlo Pazzani
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The mystery of the ice cold rose—Microbiome of an Arctic winter frost flower Microbiologyopen (IF 3.4) Pub Date : 2023-02-04 Stefan Thiele, Anna Vader, Lise Øvreås
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A new role for monomeric ParA/Soj in chromosome dynamics in Bacillus subtilis Microbiologyopen (IF 3.4) Pub Date : 2023-01-16 David M. Roberts
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A new spray-based method for the in-vitro development of dry-surface biofilms Microbiologyopen (IF 3.4) Pub Date : 2023-01-11 Esther Christine, Claude Olive, Myriam Louisin, Moustapha Dramé, Karine Marion-Sanchez
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Assessing pH-dependent activities of virulence factors secreted by Candida albicans Microbiologyopen (IF 3.4) Pub Date : 2023-01-03 Asier Ramos-Pardo, Rocío Castro-Álvarez, Guillermo Quindós, Elena Eraso, Elena Sevillano, Vladimir R. Kaberdin
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An in-house 45-plex array for the detection of antimicrobial resistance genes in Gram-positive bacteria Microbiologyopen (IF 3.4) Pub Date : 2022-12-25 Carole Kowalewicz, Michael Timmermans, David Fretin, Pierre Wattiau, Cécile Boland
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Adaptive laboratory evolution for increased temperature tolerance of the diatom Nitzschia inconspicua Microbiologyopen (IF 3.4) Pub Date : 2022-12-25 Alaina J. LaPanse, Tyson A. Burch, Jacob M. Tamburro, Jesse C. Traller, Agnieszka Pinowska, Matthew C. Posewitz