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Role of epidural fat in the local milieu: what we know and what we don’t Connect. Tissue Res. (IF 2.9) Pub Date : 2024-03-17 Zhiming Liu, Yida Wang, Xuexiao Ma, Lu Zhang, Chao Wang
Traditionally, the epidural fat (EF) is known as a physical buffer for the dural sac against the force and a lubricant facilitating the relative motion of the latter on the osseous spine. Along wit...
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S100A12 is involved in the pathology of osteoarthritis by promoting M1 macrophage polarization via the NF-κB pathway Connect. Tissue Res. (IF 2.9) Pub Date : 2024-03-16 Yi Zhang, Zihua Li, Cheng Chen, Wang Wei, Zhendong Li, Hui Huang, Haichao Zhou, Wenbao He, Jiang Xia, Bing Li, Yunfeng Yang
Osteoarthritis (OA) is a degenerative joint disease that affects millions worldwide. Synovitis and macrophage polarization are important factors in the development of OA. However, the specific comp...
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COL1A1 proximal promoter topology regulates its transcriptional response to transforming growth factor β Connect. Tissue Res. (IF 2.9) Pub Date : 2024-03-04 Elena Hitraya, Svetlana Gaidarova, Sonsoles Piera-Velazquez, Sergio A. Jimenez
The COL1A1 proximal promoter contains two GC-rich regions and two inverted CCAAT boxes. The transcription factors Sp1 and CBF bind to the GC sequence at −122 to −115 bp and the inverted CCAAT box a...
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In vitro collagen biomarkers in mechanically stimulated human tendon cells: a systematic review Connect. Tissue Res. (IF 2.9) Pub Date : 2024-02-20 Kipling Squier, Rouhollah Mousavizadeh, Faraz Damji, Charlotte Beck, Michael Hunt, Alexander Scott
The aim of this study was to comprehensively examine and summarize the available in vitro evidence regarding the relationship between mechanical stimulation and biomarkers of collagen synthesis in ...
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Macrophages in the process of osseointegration around the implant and their regulatory strategies Connect. Tissue Res. (IF 2.9) Pub Date : 2024-02-01 Xin Fang, Duo Sun, Yongli Li, Xiao Han, Yulu Gan, Junjie Jiao, Mengyuan Jiang, Heyi Gong, Yuanzheng Qi, Jinghui Zhao
To summarize and discuss macrophage properties and their roles and mechanisms in the process of osseointegration in a comprehensive manner, and to provide theoretical support and research direction...
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State transition and intercellular communication of synovial fibroblasts in response to chronic and acute shoulder injuries unveiled by single-cell transcriptomic analyses Connect. Tissue Res. (IF 2.9) Pub Date : 2024-02-01 Jiabao Ju, Mingtai Ma, Yichong Zhang, Zhentao Ding, Jianhai Chen
We aimed to investigate the heterogeneity of synovial fibroblasts and their potential to undergo cell state transitions at the resolution of single cells.We employed the single-cell RNA sequencing ...
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piR-368 promotes odontoblastic differentiation of dental papilla cells via the Smad1/5 signaling pathway by targeting Smurf1 Connect. Tissue Res. (IF 2.9) Pub Date : 2024-02-01 Xinhui Xing, Yawei She, Guohua Yuan, Guobin Yang
The important role of non-coding RNAs in odontoblastic differentiation of dental tissue-derived stem cells has been widely demonstrated; however, whether piRNA (a subclass of non-coding RNA) involv...
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The mechanism of lncRNA SNHG1 in osteogenic differentiation via miR-497-5p/ HIF1AN axis Connect. Tissue Res. (IF 2.9) Pub Date : 2024-02-01 Yuanyuan Lu, Kaihua Pan, Yunqing Zhang, Jiang Peng, Daning Cao, Xiaoming Li
The pivotal role of lncRNAs in osteoporosis progression and development necessitates a comprehensive exploration of the functional and precise molecular mechanisms underlying lncRNA SNHG1’s regulat...
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Acidosis induces significant changes to the murine supraspinatus enthesis organic matrix Connect. Tissue Res. (IF 2.9) Pub Date : 2024-02-01 Saparja Nag, Isabelle De Bruyker, Ashley Nelson, Mikayla Moody, Marla Fais, Alix C. Deymier
Rotator cuff pathology is a common musculoskeletal condition that disproportionately affects older adults, as well as patients with diabetes mellitus and chronic kidney disease. It is known that in...
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TLR4 antagonism provides short-term but not long-term clinical benefit in a full-depth cartilage defect mouse model Connect. Tissue Res. (IF 2.9) Pub Date : 2024-02-01 Ariel E. Timkovich, G. Aaron Holling, Maryam F. Afzali, John Kisiday, Kelly S. Santangelo
Cartilage injury and subsequent osteoarthritis (OA) are debilitating conditions affecting millions worldwide. As there are no cures for these ailments, novel therapies are needed to suppress diseas...
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Biomechanical assessment of gastrocnemii and Achilles tendon using MyotonPRO: in vivo measurements, and preliminary in situ measurements using formalin-fixed tissues Connect. Tissue Res. (IF 2.9) Pub Date : 2024-02-01 Xiyao Shan, Kanae Umemoto, Takuro Ishikawa, Kaori Fukushige, Takao Takeuchi, Munekazu Naito
This study aims to evaluate the reliability and validity of using MyotonPRO to quantify the mechanical properties of the muscle-tendon unit through in vivo measurements and preliminary in situ meas...
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Effects of ultrashort wave diathermy on skin wounds in rabbit ears Connect. Tissue Res. (IF 2.9) Pub Date : 2023-08-07 Peng-Peng Huang, Rui Zhang, Xiao-Feng Zhang, Zhi-Tao Xu, Du-Chun Zeng, Feng-Bao Sun, Wen-Jie Zhang
Ultrashort wave diathermy (USWD) is commonly used in diseases associated with osteoarticular and soft tissue injuries. However, while accelerating wound healing and preventing joint stiffness, ther...
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Healing potential of curcumin nanomicelles in cutaneous burn wounds: an in vitro and in vivo study Connect. Tissue Res. (IF 2.9) Pub Date : 2023-07-17 Ahmad Oryan, Esmat Alemzadeh, Soroush Mohammadi
Curcumin is the active substance of turmeric and has been shown to enhance the healing potential of burn wounds. However, its high hydrophobicity and rapid degradability are great challenges for it...
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MetaLnc9 facilitates osteogenesis of human bone marrow mesenchymal stem cells by activating the AKT pathway Connect. Tissue Res. (IF 2.9) Pub Date : 2023-07-10 Sijia Chang, Ziyao Zhuang, Chanyuan Jin
To investigate the role of MetaLnc9 in the osteogenesis of human bone marrow mesenchymal stem cells (hBMSCs).We used lentiviruses to knockdown or overexpress MetaLnc9 in hBMSCs. qRT-PCR was employe...
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The effects of weight bearing after ACL reconstruction on joint contracture in rats Connect. Tissue Res. (IF 2.9) Pub Date : 2023-07-05 Akinori Kaneguchi, Momoka Hayakawa, Atsuhiro Shimoe, Akira Takahashi, Kaoru Yamaoka, Junya Ozawa
Joint contractures after anterior cruciate ligament (ACL) reconstruction are a serious problem. Given the uncertain effects of weight bearing after ACL reconstruction on contractures, this study wa...
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Knockdown of LncRNA DICER1-AS1 arrests the cell cycle, inhibits cell proliferation, and induces cell apoptosis by regulating CDC5L nuclear transfer in osteosarcoma Connect. Tissue Res. (IF 2.9) Pub Date : 2023-06-13 Laihua Fu, Songfeng Xu, Yang Zhou, Jingyang Huang, Jin Qiu, Pengzhou Huang
DICER1-AS1 is reported to promote the progression and disturb the cell cycle in osteosarcoma; however, its mechanism has rarely been studied.DICER1-AS1 expression levels were evaluated by qPCR and ...
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A tribute to doctor Arthur Veis Connect. Tissue Res. (IF 2.9) Pub Date : 2023-06-11 Elizabeth Lux
Published in Connective Tissue Research (Vol. 64, No. 4, 2023)
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Tenogenic differentiation of human tendon-derived stem cells induced by long non-coding RNA LINCMD1 via miR-342-3p/EGR1 axis Connect. Tissue Res. (IF 2.9) Pub Date : 2023-06-07 Feng Qu, Xuezhen Shen, Ketao Wang, Chengyi Sun, Pengfei Li
ABSTRACT Background Tendon-derived stem cells (TDSCs) are proposed as a potential cell-seed for the treatment of tendon injury due to their tenogenic differentiation potential. In this work, we defined the action of long non-coding RNA (lncRNA) muscle differentiation 1 (LINCMD1) in tenogenic differentiation of human TDSCs (hTDSCs). Methods Quantitative real-time PCR (qRT-PCR) was used to assess the
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The effect of extracorporeal shock wave on joint capsule fibrosis in rats with knee extension contracture: a preliminary study Connect. Tissue Res. (IF 2.9) Pub Date : 2023-06-02 Chao Hu, Quan Bing Zhang, Feng Wang, Hua Wang, Yun Zhou
ABSTRACT The purpose of this study was to observe the therapeutic effect of extracorporeal shock wave (ESW) on extensional joint contracture of knee joint in rats and its mechanism on articular capsule fibrosis. Thirty-two SD rats were randomly divided into blank control, immobilization, natural recovery, and ESW intervention groups. Except for the control group, the left knee joints of other rats
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LPS-induced inflammation potentiates dental pulp stem cell odontogenic differentiation through C5aR and p38 Connect. Tissue Res. (IF 2.9) Pub Date : 2023-05-29 Ji-Hyun Kim, Muhammad Irfan, Md Akil Hossain, Susie Shin, Anne George, Seung Chung
ABSTRACT Aim Inflammation is a complex host response to harmful infection or injury, and it seems to play a crucial role in tissue regeneration both positively and negatively. We have previously demonstrated that the activation of the complement C5a pathway affects dentin-pulp regeneration. However, limited information is available to understand the role of the complement C5a system related to inflammation-mediated
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Notch1 is a marker for in situ resting osteocytes in a 3-dimensional gel culture model Connect. Tissue Res. (IF 2.9) Pub Date : 2023-05-25 Ying-Hui Zhou, Jia-Yu Zhu, Yue Guo, Hao-Neng Tang, Fang Wang, Junaid Iqbal, Hui-Xuan Wu, Nan Hu, Fen Xiao, Ting Wang, Long Li, Hou-De Zhou
ABSTRACT Purpose Osteocytes in vivo exhibit different functional states, but no specific marker to distinguish these is currently available. Materials and methods To simulate the differentiation process of pre-osteoblasts to osteocytes in vitro, MC3T3-E1 cells were cultured on type I collagen gel and a three-dimensional (3D) culture system was established. The Notch expression of osteocyte-like cells
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A biphasic calcium phosphate/acylated methacrylate gelatin composite hydrogel promotes osteogenesis and bone repair Connect. Tissue Res. (IF 2.9) Pub Date : 2023-05-18 Ren-Jie Xu, Jin-Jin Ma, Xiao Yu, Xiao-Qiang Zhou, Jing-Yu Zhang, Ya-Dong Li, Hui-Lin Yang, Saijilafu, Guang-Xiang Chen
ABSTRACT Purpose/Aim Bone defects caused by trauma, tumors, congenital malformation, or inflammation are very common in orthopedics. In recent years, mimicking the composition and structure of natural bone tissue has become a hot topic in biomaterial research, with the aim of developing an ideal biomaterial for bone defect transplantation. Here, the feasibility of a biphasic calcium phosphate (BCP)/acylated
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Periosteum-derived Micrografts for bone regeneration Connect. Tissue Res. (IF 2.9) Pub Date : 2023-05-17 Basel Mahardawi, Kevin A. Tompkins, Nikos Mattheos, Sirida Arunjaroensuk, Atiphan Pimkhaokham
ABSTRACT Bone regeneration is currently one of the most widely researched topics in regenerative medicine. Several bone-grafting materials have been introduced and compared. However, the limitations of the currently available grafts have led researchers to investigate new materials to be used. In contrast, the periosteum performs endogenous bone regeneration as seen in physiological bone fracture repair
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Concentration-dependent effects of leptin on osteoarthritis-associated changes in phenotype of human chondrocytes Connect. Tissue Res. (IF 2.9) Pub Date : 2023-05-17 Julia GB Primrose, Lekha Jain, Scott M Bolam, A Paul Monk, Jacob T Munro, Nicola Dalbeth, Raewyn C Poulsen
ABSTRACT Metabolic syndrome is a risk factor for osteoarthritis. Elevated leptin levels have been implicated as a potential cause of this association. Previous studies have shown that supra-physiological leptin concentrations can induce osteoarthritis-like changes in chondrocyte phenotype. Here, we tested the effects of leptin in the concentration range found in synovial fluid on chondrocyte phenotype
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Current status and future trends of reconstructing a vascularized tissue-engineered trachea Connect. Tissue Res. (IF 2.9) Pub Date : 2023-05-17 Ziqing Shen, Tian Xia, Jun Zhao, Shu Pan
ABSTRACT Alternative treatment of long tracheal defects remains one of the challenges faced by thoracic surgeons. Tissue engineering has shown great potential in addressing this regenerative medicine conundrum and the technology to make tracheal grafts using this technique is rapidly maturing, leading to unique therapeutic approaches. However, the clinical application of tissue-engineered tracheal
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Contribution of immune cells to intervertebral disc degeneration and the potential of immunotherapy Connect. Tissue Res. (IF 2.9) Pub Date : 2023-05-17 Hao Xu, Juan Li, Qinming Fei, Libo Jiang
ABSTRACT Substantial evidence supports that chronic low back pain is associated with intervertebral disc degeneration (IDD), which is accompanied by decreased cell activity and matrix degradation. The role of immune cells, especially macrophages, in a variety of diseases has been extensively studied; therefore, their role in IDD has naturally attracted widespread scholarly interest. The IVD is considered
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Comparison of methods for the isolation and culture of Migratory chondroprogenitors from Human articular cartilage Connect. Tissue Res. (IF 2.9) Pub Date : 2023-04-24 Elizabeth Vinod, Ganesh Parasuraman, Abel Livingston, Soosai Manickam Amirtham, Grace Rebekah, J Jeya Lisha, Alfred Job Daniel, Solomon Sathishkumar
ABSTRACT Purpose Resident articular stem cells isolated using a migratory assay called Migratory Chondroprogenitors (MCPs) have emerged as a promising cellular therapeutic for the treatment of cartilage pathologies. In-vivo studies using MCPs report their superiority over bone-marrow mesenchymal stem cells and chondrocytes for treating chondral defects. However, there is no consensus on their isolation
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Ovariectomy with simulated vaginal delivery to establish a rat model for pelvic organ prolapse Connect. Tissue Res. (IF 2.9) Pub Date : 2023-04-24 Tao Guo, Zhe Du, Xiu-Qi Wang, Jing-He Lang, Zhi-Jing Sun
ABSTRACT The widespread prevalence of Pelvic Organ Prolapse (POP) and the paucity of ongoing treatments prompted us to develop a unique rat model combining ovariectomy and simulated vaginal delivery. We hypothesized that the tissue changes caused by low hormone levels and mechanical stretch could complement each other. Thus, the combined model can potentially mimic the collagen metabolism of vaginal
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β-Arrestin 2 knockout prevents bone loss in response to continuous parathyroid hormone stimulation in male and female mice Connect. Tissue Res. (IF 2.9) Pub Date : 2023-04-12 Gilberto Li Feng, Marc D Grynpas, Jane Mitchell
ABSTRACT Background β-Arrestin 2 (β-arr2) binds activated parathyroid hormone (PTH) receptors stimulating internalization. PTH stimulates both anabolic and catabolic effect on bone depending on the way it is administered. Intermittent PTH stimulation increases trabecular bone formation in mice, but this is decreased in mice lacking β-arr 2, suggesting a role for β-arr 2 in the anabolic effects of PTH
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HMGB1: a potential new target for tendinopathy treatment Connect. Tissue Res. (IF 2.9) Pub Date : 2023-04-09 Panpan Lu, Yingjuan Li, Guangchun Dai, Yuanwei Zhang, Liu Shi, Ming Zhang, Hao Wang, Yunfeng Rui
ABSTRACT Tendinopathy describes a complex pathology of the tendon characterized by abnormalities in the microstructure, composition, and cellularity of the tendon, leading to pain, limitation of activity and reduced function. Nevertheless, the mechanism of tendinopathy has not been fully elucidated, and the treatment of tendinopathy remains a challenge. High mobility group box 1 (HMGB1), a highly conserved
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Retinoic acid inhibits the pyroptosis of degenerated nucleus pulposus cells by activating Sirt1-SOD2 signaling Connect. Tissue Res. (IF 2.9) Pub Date : 2023-04-02 Peng-Fei Li, Fei Xiong, Hong-Yuan Xing, Shao-Jun Hu, Ning Zhang
ABSTRACT Aim Intervertebral disc (IVD) degeneration is a common disease initiated by the degeneration of the nucleus pulposus (NP). The pyroptosis of degenerated NP cells (dNPCs) plays an important role in NP degeneration. The purpose of this study is to identify a feasible solution that can inhibit NP cell pyroptosis to therapy the degeneration of the intervertebral disc. Methods Cell viability and
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Zinc finger E-box binding homebox 2 alleviated experimental osteoarthritis in rats Connect. Tissue Res. (IF 2.9) Pub Date : 2023-03-06 Gao-Feng Zhao, Le-Bin Huang
ABSTRACT Aims Osteoarthritis (OA) is a chronic inflammatory disease, characterized by degradation of extracellular matrix, apoptosis of chondrocytes and inflammation in articular cartilage. Zinc finger E-box binding homebox 2 (ZEB2), a transcription repressor, has been demonstrated with anti-inflammatory role in some cells. The analysis from GEO data reveals that ZEB2 expression is upregulated in articular
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Dependence of crack shape in loaded articular cartilage on the collagenous structure Connect. Tissue Res. (IF 2.9) Pub Date : 2023-02-28 Yasir Al-Saffar, Eng Kuan Moo, Belinda Pingguan-Murphy, John Matyas, Rami K. Korhonen, Walter Herzog
ABSTRACT Cartilage cracks disrupt tissue mechanics, alter cell mechanobiology, and often trigger tissue degeneration. Yet, some tissue cracks heal spontaneously. A primary factor determining the fate of tissue cracks is the compression-induced mechanics, specifically whether a crack opens or closes when loaded. Crack deformation is thought to be affected by tissue structure, which can be probed by
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Dysregulation of metalloproteinases in spinal ligament degeneration Connect. Tissue Res. (IF 2.9) Pub Date : 2023-01-04 Chao Wang, Ziran Wei, Tengbo Yu, Lu Zhang
ABSTRACT Purpose Degenerative changes in the spinal ligaments, such as hypertrophy or ossification, are important pathophysiological mechanisms of secondary spinal stenosis and neurological compression. Extracellular matrix (ECM) remodeling is one of the major pathological changes in ligament degeneration, and in this remodeling, ECM proteinase-mediated degradation of elastin and collagen plays a vital
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Recent progress in the biology and physiology of BMP-8a Connect. Tissue Res. (IF 2.9) Pub Date : 2023-01-02 Jiawei Tang, Miao Tan, Siqi Liao, Mengwei Pang, Jie Li
ABSTRACT Purpose BMP-8a is a member of bone morphogenetic proteins (BMPs) and plays a regulatory role in human growth and development as a transcription regulator. This review aims to summarize the current research on the impact and mechanism of BMP-8a in female and male reproduction, formation and eruption of teeth, bone and cartilage development, tissue differentiation, disease occurrence, progression
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Tendon properties in a mouse model of severe osteogenesis imperfecta Connect. Tissue Res. (IF 2.9) Pub Date : 2022-12-28 Larissa Sinkam, Iris Boraschi-Diaz, René B. Svensson, Michael Kjaer, Svetlana V. Komarova, Raynald Bergeron, Frank Rauch, Louis-Nicolas Veilleux
ABSTRACT Purpose/Aim of the study Osteogenesis imperfecta is a heritable bone disorder that is usually caused by mutations in collagen type I encoding genes. The impact of such mutations on tendons, a structure with high collagen type I content, remains largely unexplored. We hypothesized that tendon properties are abnormal in the context of a mutation affecting collagen type I. The main purpose of
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Role of pro-inflammatory interleukins in osteoarthritis: a narrative review Connect. Tissue Res. (IF 2.9) Pub Date : 2022-12-21 Asma Inès Sana Jrad, Maha Trad, Wafaa Bzeih, Georges El Hasbani, Imad Uthman
ABSTRACT Purpose This manuscript will summarize the role of pro-inflammatory cytokines and tackle newly discussed ones within the scope of OA pathogenesis as mentioned in the recent literature. This will allow for a better understanding of the mechanisms behind such a complicated disease. Material and methods Relevant articles were obtained by searching key terms including “pro-inflammatory cytokines
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miR-34a-5p facilitates osteogenic differentiation of bone marrow mesenchymal stem cells and modulates bone metabolism by targeting HDAC1 and promoting ER-α transcription Connect. Tissue Res. (IF 2.9) Pub Date : 2022-12-20 Dawei Sun, Yuhui Chen, Xiaochun Liu, Guoying Huang, Guoyun Cheng, Chaoqun Yu, Jia Fang
ABSTRACT Objective Metabolism is essential for bone development. The expressions of catabolic markers in chondrocytes show association with miR-34a-5p. This study discussed the mechanism by which miR-34a-5p regulates osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) as well as bone metabolism. Methods Expressions of BMSC surface markers were determined via flow cytometry. Osteogenic
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Teriparatide prevented synovial inflammation and cartilage destruction in mice with DMM Connect. Tissue Res. (IF 2.9) Pub Date : 2022-12-20 Xu Liang, Sen-Rui Li, Xin-Xin Zhang, Shi-Hao He, Shan-Shan Li, Tian-Fang Li
ABSTRACT Aim Emerging data have demonstrated that low-grade inflammation in osteoarthritis, a long-held degenerative disease. The inflamed synovium produces various cytokines that induce cartilage destruction and joint pain. A previous study showed that teriparatide, an FDA approved anti-osteoporotic drug, may enhance cartilage repair. Our study focuses on its role in OA synovitis. Materials and Methods
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Mechanics and differential healing outcomes of small and large defect injuries of the tendon-bone attachment in the rat rotator cuff Connect. Tissue Res. (IF 2.9) Pub Date : 2022-12-16 Anna Lia Sullivan, Ryan C. Locke, Rachel K. Klink, Connor C. Leek, James E. Carpenter, Megan L. Killian
ABSTRACT Introduction Rotator cuff tear size affects clinical outcomes following rotator cuff repair and is correlated with the risk of recurrent tendon defects. This study aimed to understand if and how the initial defect size influences the structural and mechanical outcomes of the injured rotator cuff attachment in vivo. Methods Full-thickness punch injuries of the infraspinatus tendon-bone attachment
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280 mT static magnetic field promotes the growth of postpartum condylar cartilage Connect. Tissue Res. (IF 2.9) Pub Date : 2022-12-05 Yiwen Xiao, Qinhao Shen, Weihao Li, Yibo Zhang, Kang Yin, Yanhua Xu
ABSTRACT Purpose Functional appliances made of permanent magnets have been used in jaw orthopedic treatment. However, whether the static magnetic field (SMF) generated by permanent magnets promotes the developmental sequence of condylar cartilage and thus promotes the growth of the mandible remains to be studied. The aim of this study was to investigate the effects of 280 mT SMF on postnatal condylar
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Formyl peptide receptors in bone research Connect. Tissue Res. (IF 2.9) Pub Date : 2022-11-28 Mark A. Lantieri, Jose R. Perdomo Trejo, Quang Le, Abhijit Dighe, Quanjun Cui, Xinlin Yang
ABSTRACT Purpose/Aim of the Study The formyl peptide receptor (FPR) participates in the immune response, with roles in infection and inflammation. In this review article, we summarize the current literature on these roles before discussing the function of FPRs in the pathogenesis of musculoskeletal disorders including osteoarthritis (OA), degenerative disc disease (DDD), and rheumatoid arthritis (RA)
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Comparative proteomics analysis of transforming growth factor-beta1-overexpressed human dental pulp stem cell-derived secretome on CD44-mediated fibroblast activation via canonical smad signal pathway Connect. Tissue Res. (IF 2.9) Pub Date : 2022-11-24 H. Salkin, M. B. Acar, Z. B. Gonen, K. E. Basaran, S. Ozcan
ABSTRACT Purpose The aim of this study investigates whether the secretome collected from human dental pulp stem cells (hDPSCs) transfected with transforming growth factor-beta1 (TGF-β1) is related to CD44 expression of fibroblasts and canonical smad signaling pathway via proteomic analyzes. Materials and Methods In order to obtain secretome, hDPSCs were conditioned with serum-free alpha-MEM in an incubator
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Sclerostin antibody promotes bone formation through the Wnt/β-catenin signaling pathway in femoral trochlear after patellar instability Connect. Tissue Res. (IF 2.9) Pub Date : 2022-11-15 Chenyue Xu, Gang Ji, Xiaobo Chen, Lirong Yan, Tuwan Liang, Junle Liu, Fei Wang
ABSTRACT Purpose The molecular mechanism of patellar instability (PI) remains unknown. The purpose of this study was to explore the function of SOST/sclerostin in PI and examine the effect of sclerostin antibody (Scl-Ab). Materials and Methods We randomly divided 60 male 3-week-old C57Bl/6 mice into four groups: sham, PI, Scl-Ab intraperitoneal injection (Scl-Ab IP), Scl-Ab intraarticular injection
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The effects of the amount of weight bearing on articular cartilage early after ACL reconstruction in rats Connect. Tissue Res. (IF 2.9) Pub Date : 2022-11-05 Akinori Kaneguchi, Kaoru Yamaoka, Junya Ozawa
ABSTRACT Purpose Osteoarthritis that develops after anterior cruciate ligament (ACL) reconstruction is a critical issue. We examined the effects of the amount of weight bearing early after ACL reconstruction on articular cartilage. Materials and Methods Rats were divided into groups according to the treatment received: untreated control, ACL reconstruction (ACLR), ACL reconstruction plus hindlimb unloading
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Early patellofemoral cartilage and bone pathology in a rat model of noninvasive anterior cruciate ligament rupture Connect. Tissue Res. (IF 2.9) Pub Date : 2022-11-01 Mackenzie M. Fleischer, Samantha E. Hartner, Michael D. Newton, Kevin C. Baker, Tristan Maerz
ABSTRACT Objective Anterior cruciate ligament rupture (ACLR) is a risk factor for the development of post-traumatic osteoarthritis (PTOA). While PTOA in the tibiofemoral joint compartment is well-characterized, very little is known about pathology in the patellofemoral compartment after ACL injury. Here, we evaluated the extent to which ACLR induces early patellofemoral joint damage in a rat model
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Impact of elevated serum advanced glycation end products and exercise on intact and injured murine tendons Connect. Tissue Res. (IF 2.9) Pub Date : 2022-10-25 Shivam H. Patel, Chad C. Carroll
ABSTRACT Overview: Delayed tendon healing is a significant clinical challenge for those with diabetes. We explored the role of advanced glycation end-products (AGEs), a protein modification present at elevated levels in serum of individuals with diabetes, on injured and intact tendons using a mouse model. Cell proliferation following tissue injury is a vital component of healing. Based on our previous
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The effects of epigenetic modifications on bone remodeling in age-related osteoporosis Connect. Tissue Res. (IF 2.9) Pub Date : 2022-10-21 Wenyue Yu, He-Ling Wang, Jianying Zhang, Chengcheng Yin
ABSTRACT Purpose: As the population ages, there is an increased risk of fracture and morbidity diseases associated with aging, such as age-related osteoporosis and other bone diseases linked to aging skeletons. Results: Several bone-related cells, including multipotent bone mesenchymal stem cells, osteoblasts that form bone tissue, and osteoclasts that break it down, are in symbiotic relationships
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Oncostatin M attenuates tumor necrosis factor-α-induced synthesis of macrophage-colony stimulating factor via suppression of Akt in osteoblasts Connect. Tissue Res. (IF 2.9) Pub Date : 2022-08-19 Tomoyuki Hioki, Gen Kuroyanagi, Rie Matsushima-Nishiwaki, Osamu Kozawa, Haruhiko Tokuda
ABSTRACT Background Oncostatin M produced by osteal macrophages, a cytokine that belongs to the interleukin-6 family, is implicated in bone fracture healing. Macrophage colony-stimulating factor (M-CSF) secreted from osteoblasts plays an important role in osteoclastogenesis. We have previously reported that tumor necrosis factor-α (TNF-α), a potent bone resorptive agent, stimulates the activation of
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The adaptive response of the mandibular condyle to increased load is disrupted by ADAMTS5 deficiency Connect. Tissue Res. (IF 2.9) Pub Date : 2022-08-01 Sarah C Porto, Alexandra Rogers-DeCotes, Emmaline Schafer, Christine B Kern
ABSTRACT Objective To determine the impact of increased load on the temporomandibular joint (TMJ) from mice deficient in the extracellular matrix protease ADAMTS5. Materials and Methods Wire springs exerting 0.5 N for 1 h/day for 5 days (Adamts5+/+ -n = 18; Adamts5−/− n = 19) or 0.8 N for 1 h/day for 10 days (Adamts5+/+-n = 18; Adamts5−/− n = 17) were used to increase murine TMJ load. Safranin O-staining
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Impact of aging on tendon homeostasis, tendinopathy development, and impaired healing Connect. Tissue Res. (IF 2.9) Pub Date : 2022-07-28 Antonion Korcari, Samantha J. Przybelski, Anne Gingery, Alayna E. Loiselle
ABSTRACT Aging is a complex and progressive process where the tissues of the body demonstrate a decreased ability to maintain homeostasis. During aging, there are substantial cellular and molecular changes, with a subsequent increase in susceptibility to pathological degeneration of normal tissue function. In tendon, aging results in well characterized alterations in extracellular matrix (ECM) structure
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Genome-wide association study of handgrip strength in the Northern Chinese adult twins Connect. Tissue Res. (IF 2.9) Pub Date : 2022-07-25 Jia Luo, Tianhao Zhang, Weijing Wang, Dongfeng Zhang
ABSTRACT Purpose Currently, new loci related to handgrip strength have been identified in genome-wide association studies. However, this topic is an understudied area in the Chinese population. Materials and Methods A total of 135 dizygotic twin pairs recruited from the Qingdao Twin Registry system were included in the present study. Using GEMMA, VEAGSE2, and PASCAL software for SNP-based analysis
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The role of substance P on maintaining ligament homeostasis by inhibiting endochondral ossification during osteoarthritis progression Connect. Tissue Res. (IF 2.9) Pub Date : 2022-07-20 Maya Tokumoto, Tomoyuki Nakasa, Yoshiko Shirakawa, Akinori Nekomoto, Yasunari Ikuta, Masakazu Ishikawa, Shigeru Miyaki, Nobuo Adachi
ABSTRACT Purpose Osteoarthritis (OA) is characterized by the degeneration of various tissues, including ligaments. However, pathological changes such as chondrogenesis and ossification in ligaments during OA are still unclear. Substance P (SP), a neuropeptide, has various functions including bone metabolism. This study aimed to analyze the expression and function of SP in OA ligaments, and the therapeutic
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Clinical and laboratory findings following transplantation of allogeneic adipose-derived mesenchymal stromal cells in knee osteoarthritis, a brief report Connect. Tissue Res. (IF 2.9) Pub Date : 2022-07-20 Bahareh Sadri, Atena Tamimi, Shirin Nouraein, Abolfazl Bagheri Fard, Javad Mohammadi, Mehdi Mohammadpour, Mohammad Hassanzadeh, Amir Bajouri, Hoda Madani, Maryam Barekat, Shahedeh Karimi Torshizi, Mahrooz Malek, Maede Ghorbani Liastani, Alireza Beheshti Maal, Maryam Niknejadi, Massoud Vosough
ABSTRACT Background Mesenchymal stromal cells (MSCs) injection has been proposed as an innovative treatment for knee osteoarthritis (KOA). Since, allogeneic MSCs can be available as off-the-shelf products, they are preferable in regenerative medicine. Among different sources for MSCs, adipose-derived MSCs (AD-MSCs) appear to be more available. Methods Three patients with KOA were enrolled in this study
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Post-injury tendon mechanics are not affected by tamoxifen treatment Connect. Tissue Res. (IF 2.9) Pub Date : 2022-07-11 Zakary M. Beach, Ashley K. Fung, Stephanie N. Weiss, Louis J. Soslowsky
ABSTRACT Purpose A growing interest in the mechanisms that govern tendon healing has resulted in the develop-ment of tools, such as the tamoxifen-inducible mouse knockdown model, to address these questions. However, tamoxifen is a selective estrogen receptor modulator and may interfere with the tendon healing process. The objective of this study was to evaluate the effects of tamoxifen on post-injury
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Effect of simvastatin on osteogenesis of the extremity bones in aging rats Connect. Tissue Res. (IF 2.9) Pub Date : 2022-07-11 Mengran Wang, Haowei Li, Jiaxin Tang, Yue Xi, Shiyi Chen, Ming Liu
ABSTRACT Purpose Simvastatin is a prodrug of the potent 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor. The main purpose of the current study is to assess the accurate function of simvastatin on osteoporosis of extremity bones in aging rats. Materials and methods Fifty 15-month-old SD rats were divided into five groups (four simvastatin groups and one control group). The rats in four simvastatin
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The BMP and FGF pathways reciprocally regulate odontoblast differentiation Connect. Tissue Res. (IF 2.9) Pub Date : 2022-07-11 Karo Parsegian
ABSTRACT Purpose Previous studies demonstrated that the exposure of primary dental pulp (DP) cultures to fibroblast growth factor 2 (FGF2) between days 3–7 exerted significant and long-lasting stimulatory effects on odontoblast differentiation and Dspp expression. These effects involved the increased expression of components of bone morphogenetic protein (BMP) signaling and were reverted by a BMP inhibitor
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Role of Nrf2 and HO-1 in intervertebral disc degeneration Connect. Tissue Res. (IF 2.9) Pub Date : 2022-06-23 Cang-Yu Zhang, Xu-Chang Hu, Guang-Zhi Zhang, Ming-Qiang Liu, Hai-Wei Chen, Xue-Wen Kang
ABSTRACT Intervertebral disc degeneration (IDD) is a common age-related disease with clinical manifestations of lumbar and leg pain and limited mobility. The pathogenesis of IDD is mainly mediated by the death of intervertebral disc (IVD) cells and the imbalance of extracellular matrix (ECM) synthesis and degradation. Oxidative stress and inflammatory reactions are the important factors causing this
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RANKL interferes with osteoclastogenesis in PEG-fused U937 cells through LGR4 Connect. Tissue Res. (IF 2.9) Pub Date : 2022-06-21 Juan A. Arteaga, Carlos A. Guerrero
ABSTRACT Introduction RANKL plays an important role in the differentiation and maturation process of preosteoclast cells. The osteoclast is a multinucleated cell that can have various sizes and a variable number of nuclei. However, there are no models that allow us to understand how successive cell fusions have a limit, or how cell fusion is regulated. Methodology The present investigation was aimed
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Metformin reduces myogenic contracture and myofibrosis induced by rat knee joint immobilization via AMPK-mediated inhibition of TGF-β1/Smad signaling pathway Connect. Tissue Res. (IF 2.9) Pub Date : 2022-06-20 Feng Wang, Chen Xu Zhou, Zhi Zheng, Du Juan Li, Wen Li, Yun Zhou
ABSTRACT Purpose The two structural components contributing to joint contracture formation are myogenic and arthrogenic contracture, and myofibrosis is an important part of myogenic contracture. Myofibrosis is a response to long-time immobilization and is described as a condition with excessive deposition of endomysial and perimysial connective tissue components in skeletal muscle. The purpose of this