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  • Minireview: Insights into anti-interferon-γ autoantibodies
    Exp. Biol. Med. (IF 3.139) Pub Date : 2021-01-11
    Kriangkrai Chawansuntati; Kritsadee Rattanathammethee; Jiraprapa Wipasa

    The association between the presence of anti-interferon-γ autoantibodies and the onset of immunodeficiency with intracellular infections has been clearly established. No standard regimen to control the production of these pathogenic autoantibodies, apart from antimicrobial therapy to eliminate infections, contributes to the medical burden of this syndrome, which sometimes has a fatal outcome. In this

    更新日期:2021-01-12
  • SIRT3 deficiency increases mitochondrial oxidative stress and promotes migration of retinal pigment epithelial cells
    Exp. Biol. Med. (IF 3.139) Pub Date : 2021-01-10
    Jing-Xian Wang; Yuan Yang; Wen-Ying Li

    Retinal pigment epithelial cells are closely associated with the pathogenesis of diabetic retinopathy. The mechanism by which diabetes impacts retinal pigment epithelial cell function is of significant interest. Sirtuins are an important class of proteins that primarily possess nicotinamide adenine dinucleotide-dependent deacetylases activity and involved in various cellular physiological and pathological

    更新日期:2021-01-11
  • White matter abnormalities in fetal alcohol spectrum disorders: Focus on axon growth and guidance
    Exp. Biol. Med. (IF 3.139) Pub Date : 2021-01-10
    Erin Mathews; Kevyn Dewees; Deborah Diaz; Carlita Favero

    Fetal Alcohol Spectrum Disorders (FASDs) describe a range of deficits, affecting physical, mental, cognitive, and behavioral function, arising from prenatal alcohol exposure. FASD causes widespread white matter abnormalities, with significant alterations of tracts in the cerebral cortex, cerebellum, and hippocampus. These brain regions present with white-matter volume reductions, particularly at the

    更新日期:2021-01-11
  • Conformation and protein interactions of intramolecular DNA and phosphorothioate four-way junctions
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-12-20
    Maria Troisi; Mitchell Klein; Andrew C Smith; Gaston Moorhead; Yonatan Kebede; Raymond Huang; Elliott Parker; Hector Herrada; Elizabeth Wade; Samara Smith; Payson Broome; Jonah Halsell; Louis Estevez; Anthony J Bell, Jr

    The objectives of this study are to evaluate the structure and protein recognition features of branched DNA four-way junctions in an effort to explore the therapeutic potential of these molecules. The classic immobile DNA 4WJ, J1, is used as a matrix to design novel intramolecular junctions including natural and phosphorothioate bonds. Here we have inserted H2-type mini-hairpins into the helical termini

    更新日期:2020-12-21
  • COVID-19 global pandemic planning: Dry heat incubation and ambient temperature fail to consistently inactivate SARS-CoV-2 on N95 respirators
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-12-20
    Douglas J Perkins; Robert A Nofchissey; Chunyan Ye; Nathan Donart; Alison Kell; Ivy Foo-Hurwitz; Timothy Muller; Steven B Bradfute

    The ongoing pandemic of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has placed a substantial strain on the supply of personal protective equipment, particularly the availability of N95 respirators for frontline healthcare personnel. These shortages have led to the creation of protocols to disinfect and reuse potentially contaminated personal protective equipment. A simple

    更新日期:2020-12-21
  • Post-treatment with glycyrrhizin can attenuate hepatic mitochondrial damage induced by acetaminophen in mice
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-12-20
    Xue-Liang Dang; Long-Fei Yang; Lei Shi; Long-Fei Li; Ping He; Jie Chen; Bei-Jie Zheng; Peng Yang; Ai-Dong Wen

    Overdose of acetaminophen (APAP) is responsible for the most cases of acute liver failure worldwide. Hepatic mitochondrial damage mediated by neuronal nitric oxide synthase- (nNOS) induced liver protein tyrosine nitration plays a critical role in the pathophysiology of APAP hepatotoxicity. It has been reported that pre-treatment or co-treatment with glycyrrhizin can protect against hepatotoxicity through

    更新日期:2020-12-21
  • Immunomodulatory effects of mesenchymal stem cells for the treatment of cardiac allograft rejection
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-12-16
    Zhichao Wu; Jialiang Liang; Wei Huang; Lin Jiang; Christian Paul; Xiang Gao; Perwez Alam; Onur Kanisicak; Meifeng Xu; Yigang Wang

    Heart transplantation continues to be the gold standard clinical intervention to treat patients with end-stage heart failure. However, there are major complications associated with this surgical procedure that reduce the survival prognosis of heart transplant patients, including allograft rejection, malignancies, infections, and other complications that arise from the use of broad-spectrum immunosuppression

    更新日期:2020-12-17
  • Analyses of short-chain fatty acids and exhaled breath volatiles in dietary intervention trials for metabolic diseases
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-12-16
    Jisun HJ Lee; Jiangjiang Zhu

    As an alternative to pharmacological treatment to diseases, lifestyle interventions, such as dietary changes and physical activities, can help maintain healthy metabolic conditions. Recently, the emerging analyses of volatile organic compounds (VOCs) from breath and short-chain fatty acids (SCFAs) from plasma/feces have been considered as useful tools for the diagnosis and mechanistic understanding

    更新日期:2020-12-17
  • Identification of histone acetylation in a murine model of allergic asthma by proteomic analysis
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-12-16
    Yuan Ren; Menglu Li; Shiyao Bai; Lingfei Kong; Xinming Su

    The pathogenesis of asthma is closely related to histone acetylation modification, but the specific acetylation sites related to this process remain indistinct. Herein, our study sought to identify differentially modified acetylation sites and their expression distribution in cells involved in asthma in lung tissues. The airway hyper-responsiveness, inflammation, and remodeling were assessed by non-invasive

    更新日期:2020-12-17
  • Roles of kisspeptin in IVF/ICSI-treated infertile women and in human granulosa cells
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-12-16
    Lixian Qin; Chantacha Sitticharoon; Somsin Petyim; Issarawan Keadkraichaiwat; Rungnapa Sririwichitchai; Pailin Maikeaw; Malika Churintaraphan; Chanakarn Sripong

    Kisspeptin, a crucial central regulator of reproduction, has been used as a trigger in in vitro fertilization (IVF) treatment. This study aimed to investigate the roles of kisspeptin in IVF treatment in infertile females (n = 30); and in steroidogenesis in human granulosa-like tumor cell line (KGN). In the human study, blood was collected at three time points including (1) the beginning of gonadotropin

    更新日期:2020-12-17
  • High-throughput genotyping assays for identification of glycophorin B deletion variants in population studies
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-12-16
    Dominic SY Amuzu; Kirk A Rockett; Ellen M Leffler; Felix Ansah; Nicholas Amoako; Collins M Morang’a; Christina Hubbart; Kate Rowlands; Anna E Jeffreys; Lucas N Amenga-Etego; Dominic P Kwiatkowski; Gordon A Awandare

    Glycophorins are the most abundant sialoglycoproteins on the surface of human erythrocyte membranes. Genetic variation in glycophorin region of human chromosome 4 (containing GYPA, GYPB, and GYPE genes) is of interest because the gene products serve as receptors for pathogens of major public health interest, including Plasmodium sp., Babesia sp., Influenza virus, Vibrio cholerae El Tor Hemolysin, and

    更新日期:2020-12-16
  • Genomic analysis of SARS-CoV-2 reveals local viral evolution in Ghana
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-12-16
    Joyce M Ngoi; Peter K Quashie; Collins M Morang'a; Joseph HK Bonney; Dominic SY Amuzu; Selassie Kumordjie; Ivy A Asante; Evelyn Y Bonney; Miriam Eshun; Linda Boatemaa; Vanessa Magnusen; Erasmus N Kotey; Nicaise T Ndam; Frederick Tei-Maya; Augustina K Arjarquah; Evangeline Obodai; Isaac D Otchere; Yaw Bediako; Joe K Mutungi; Lucas N Amenga-Etego; John K Odoom; Abraham K Anang; George B Kyei; Bright

    The confirmed case fatality rate for the coronavirus disease 2019 (COVID-19) in Ghana has dropped from a peak of 2% in March to be consistently below 1% since May 2020. Globally, case fatality rates have been linked to the strains/clades of circulating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) within a specific country. Here we present 46 whole genomes of SARS-CoV-2 circulating in

    更新日期:2020-12-16
  • COVID-19 global pandemic planning: Performance and electret charge of N95 respirators after recommended decontamination methods
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-12-16
    Anne M Grillet; Martin B Nemer; Steven Storch; Andres L Sanchez; Edward S Piekos; Jonathan Leonard; Ivy Hurwitz; Douglas J Perkins

    Shortages of N95 respirators for use by medical personnel have driven consideration of novel conservation strategies, including decontamination for reuse and extended use. Decontamination methods listed as promising by the Centers for Disease Control and Prevention (CDC) (vaporous hydrogen peroxide (VHP), wet heat, ultraviolet irradiation (UVI)) and several methods considered for low resource environments

    更新日期:2020-12-16
  • YAP activation in melanoma contributes to anoikis resistance and metastasis
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-12-13
    Bei Zhao; Jun Xie; Xiyuan Zhou; Lixia Zhang; Xiankui Cheng; Chenglin Liang

    Melanoma is inherently heterogeneous, providing resistance to apoptosis. Anoikis resistance is a hallmark feature of metastatic melanoma to escape apoptosis when cells lose contact with adjacent cells or extracellular matrix. The yes-associated protein transcription co-activator is the effector of Hippo pathway. Herein, we investigated the function of yes-associated protein in anoikis resistance of

    更新日期:2020-12-14
  • Functional intrinsic optical signal imaging for objective optoretinography of human photoreceptors
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-12-13
    Taeyoon Son; Tae-Hoon Kim; Guangying Ma; Hoonsup Kim; Xincheng Yao

    Functional mapping of photoreceptor physiology is important for better disease diagnosis and treatment assessment. Fast intrinsic optical signal (IOS), which arises before light-evoked pupillary response, promises a unique biomarker of photoreceptor physiology for objective optoretinography with high resolution. This study is to test the feasibility of non-mydriatic IOS mapping of retinal photoreceptors

    更新日期:2020-12-14
  • Rapid and ultrasensitive detection of circulating human papillomavirus E7 cell-free DNA as a cervical cancer biomarker
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-12-13
    Phetploy Rungkamoltip; Sasithon Temisak; Kitiya Piboonprai; Deanpen Japrung; Pattanapong Thangsunan; Saranya Chanpanitkitchot; Woraphot Chaowawanit; Nutthaporn Chandeying; Siriwan Tangjitgamol; Tawin Iempridee

    Circulating cell-free DNA (cfDNA) has attracted attention as a non-invasive biomarker for diagnosing and monitoring various cancers. Given that human papillomavirus (HPV) DNA integration and overexpression of E6/E7 oncogenes are pivotal events for carcinogenesis, we sought to determine if HPV E7 cfDNA could serve as a specific biomarker for cervical cancer detection. We applied droplet digital PCR

    更新日期:2020-12-14
  • Characterizing disease progression of nonalcoholic steatohepatitis in Leptin-deficient rats by integrated transcriptome analysis
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-12-10
    Ping Lu; Guang Yang; Lichun Jiang; Wen He; Wanwan Wu; Lingbin Qi; Shijun Shen; Junhua Rao; Peng Zhang; Zhigang Xue; Cizhong Jiang; Guoping Fan; Xianmin Zhu

    Nonalcoholic steatohepatitis (NASH) is an aggressive liver disease threatening human health, yet no medicine is developed to treat this disease. In this study, we first discovered that Leptin mutant rats (LepΔI14/ΔI14) exhibit characteristic NASH phenotypes including steatosis, lymphocyte infiltration, and ballooning after postnatal week 16. We then examined NASH progression by performing an integrated

    更新日期:2020-12-11
  • Nomograms-based prediction of overall and cancer-specific survivals for patients with chromophobe renal cell carcinoma
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-12-10
    Chunyang Chen; Xinyu Geng; Rui Liang; Dongze Zhang; Meiyun Sun; Guangbo Zhang; Jianquan Hou

    This study built and tested two effective nomograms for the purpose of predicting cancer-specific survival and overall survival of chromophobe renal cell carcinoma (chRCC) patients. Multivariate Cox regression analysis was employed to filter independent prognostic factors predictive of cancer-specific survival and overall survival, and the nomograms were built based on a training set incorporating

    更新日期:2020-12-11
  • The neurovascular extracellular matrix in health and disease
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-12-10
    Aric F Logsdon; Elizabeth M Rhea; May Reed; William A Banks; Michelle A Erickson

    The blood–brain barrier (BBB) is a vital interface that supports normal brain functions. Endothelial cells (ECs) are the main component of the BBB and are highly specialized to govern the transfer of substances into brain. The EC lumen is enmeshed with an extracellular matrix (ECM), known as the endothelial glycocalyx layer (EGL). The lumen-facing EGL is primarily comprised of proteoglycans (PGs) and

    更新日期:2020-12-11
  • Protease-activated receptor 1 as a potential therapeutic target for COVID-19
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-12-10
    Emanuel S. Rovai; Tomaz Alves; Marinella Holzhausen

    Acute respiratory disease caused by a novel coronavirus (SARS-CoV-2) has spread all over the world, since its discovery in 2019, Wuhan, China. This disease is called COVID-19 and already killed over 1 million people worldwide. The clinical symptoms include fever, dry cough, dyspnea, headache, dizziness, generalized weakness, vomiting, and diarrhea. Unfortunately, so far, there is no validated vaccine

    更新日期:2020-12-11
  • FOXM1 activates JAK1/STAT3 pathway in human osteoarthritis cartilage cell inflammatory reaction
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-12-09
    Runming Zeng; Xiaohui Lu; Jing Lin; Zhijie Ron; Jiezhuang Fang; Zewa Liu; Wanting Zeng

    Osteoarthritis (OA), the most prevalent form of arthritis disease, is characterized by destruction of articular cartilage, osteophyte development, and sclerosis of subchondral bone. Transcription factors Janus kinase 1/signal transducer and activator of transcription 3 (JAK1/STAT3) and Forkhead box M1 (FOXM1) are key mediators of this inflammatory reaction. In this study, we investigated the interaction

    更新日期:2020-12-10
  • Integrating photoacoustic microscopy with other imaging technologies for multimodal imaging
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-12-09
    Arash Dadkhah; Shuliang Jiao

    As a hybrid optical microscopic imaging technology, photoacoustic microscopy images the optical absorption contrasts and takes advantage of low acoustic scattering of biological tissues to achieve high-resolution anatomical and functional imaging. When combined with other imaging modalities, photoacoustic microscopy-based multimodal technologies can provide complementary contrast mechanisms to reveal

    更新日期:2020-12-10
  • COMMD1 upregulation is involved in copper efflux from ischemic hearts
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-12-06
    Chen Li; Tao Wang; Ying Xiao; Kui Li; Xia Meng; Y James Kang

    Copper depletion is associated with myocardial ischemic infarction, in which copper metabolism MURR domain 1 (COMMD1) is increased. The present study was undertaken to test the hypothesis that the elevated COMMD1 is responsible for copper loss from the ischemic myocardium, thus worsening myocardial ischemic injury. Mice (C57BL/6J) were subjected to left anterior descending coronary artery permanent

    更新日期:2020-12-07
  • Redox treatment ameliorates diabetes mellitus-induced skin flap necrosis via inhibiting apoptosis and promoting neoangiogenesis
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-12-06
    Yeon S Kim; Hye-Young Lee; Jeon Y Jang; Hye R Lee; Yoo S Shin; Chul-Ho Kim

    Intractable wound healing is the habitual problem of diabetes mellitus. High blood glucose limits wound healing by interrupting inflammatory responses and inhibiting neoangiogenesis. Oxidative stress is commonly thought to be a major pathogenic cause of diabetic complications. Edaravone (3-methyl-1-phenyl-2-pyrazolin-5-one, EDV) is a free radical scavenger which suppress oxidative stress. This study

    更新日期:2020-12-07
  • Importance of extracellular vesicles in hypertension
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-12-06
    Zhi Z Liu; Pedro A Jose; Jian Yang; Chunyu Zeng

    Hypertension affects approximately 1.13 billion adults worldwide and is the leading global risk factor for cardiovascular, cerebrovascular, and kidney diseases. There is emerging evidence that extracellular vesicles participate in the development and progression of hypertension. Extracellular vesicles are membrane-enclosed structures released from nearly all types of eukaryotic cells. During their

    更新日期:2020-12-07
  • Nitric oxide and sickle cell disease—Is there a painful connection?
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-12-06
    Lillian Hallmark; Luis EF Almeida; Sayuri Kamimura; Meghann Smith; Zenaide MN Quezado

    Sickle cell disease is the most common hemoglobinopathy and affects millions worldwide. The disease is associated with severe organ dysfunction, acute and chronic pain, and significantly decreased life expectancy. The large body of work demonstrating that hemolysis results in rapid consumption of the endogenous vasodilator nitric oxide, decreased nitric oxide production, and promotion of vaso-occlusion

    更新日期:2020-12-07
  • Store-operated calcium entry: Pivotal roles in renal physiology and pathophysiology
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-29
    Sarika Chaudhari; Robert T Mallet; Parisa Y Shotorbani; Yu Tao; Rong Ma

    Research conducted over the last two decades has dramatically advanced the understanding of store-operated calcium channels (SOCC) and their impact on renal function. Kidneys contain many types of cells, including those specialized for glomerular filtration (fenestrated capillary endothelium, podocytes), water and solute transport (tubular epithelium), and regulation of glomerular filtration and renal

    更新日期:2020-12-01
  • Super-resolution ophthalmoscopy: Virtually structured detection for resolution improvement in retinal imaging
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-27
    Xincheng Yao; Rongwen Lu; Benquan Wang; Yiming Lu; Tae-Hoon Kim

    Quantitative retinal imaging is essential for advanced study and clinical management of eye diseases. However, spatial resolution of retinal imaging has been limited due to available numerical aperture and optical aberration of the ocular optics. Structured illumination microscopy has been established to break the diffraction-limit resolution in conventional light microscopy. However, practical implementation

    更新日期:2020-11-27
  • Altered gene expression in glycolysis–cholesterol synthesis axis correlates with outcome of triple-negative breast cancer
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-27
    Peng-Cheng Zhong; Rong Shu; Hui-Wen Wu; Zhi-Wen Liu; Xiao-Ling Shen; Ying-Jie Hu

    Identification of molecular subtypes of clinically resectable triple-negative breast cancer (TNBC) is of great importance to achieve better clinical outcomes. Inter- and intratumor metabolic heterogeneity improves cancer survival, and the interaction of various metabolic pathways may affect treatment outcome of TNBC. We speculated that TNBC can be categorized into prognostic metabolic subtype according

    更新日期:2020-11-27
  • Elevated RUNX1 is a prognostic biomarker for human head and neck squamous cell carcinoma
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-26
    Xiaodong Feng; Zhiwei Zheng; Yi Wang; Guanghui Song; Lu Wang; Zhijun Zhang; Jinxia Zhao; Qing Wang; Limin Lun

    Runt-related transcription factors regulate many developmental processes such as proliferation and differentiation. In this study, the function of the runt-related transcription factor 1 (RUNX1) was investigated in head and neck squamous cell carcinoma (HNSCC). Our results show that RUNX1 expression was elevated in HNSCC patients, which was greatly correlated with the N stage, tumor size, and American

    更新日期:2020-11-27
  • The clinical phenotype of bronchiectasis and its clinical guiding implications
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-26
    Li Gao; Ke-Ru Qin; Ting Li; Hai-Long Wang; Min Pang

    Bronchiectasis is a chronic airway disease with abnormal and persistent bronchial dilatation caused by a variety of reasons. In recent years, numerous reports have shown that bronchiectasis is heterogeneous, the clinical characteristics of patients with different phenotypes are different, and the efficacy of a treatment regimen may vary greatly in patients with different bronchiectasis phenotypes.

    更新日期:2020-11-27
  • Regional heterogeneity in rat Peyer’s patches through whole transcriptome analysis
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-25
    Charles L Phillips; Bradley A Welch; Michael R Garrett; Bernadette E Grayson

    Peyer’s patches are gut-associated lymphoid tissue located throughout the intestinal wall. Peyer’s patches consist of highly organized ovoid-shaped follicles, classified as non-encapsulated lymphatic tissues, populated with B cells, T cells, macrophages, and dendritic cells and function as an organism’s intestinal surveillance. Limited work compares the gene profiles of Peyer’s patches derived from

    更新日期:2020-11-25
  • Identification and analysis of immune-related subtypes of hepatocellular carcinoma
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-24
    Qimeng Wang; Jin Huang; Huihua Zhang; Huan Liu; Min Yu

    Hepatocellular carcinoma is a malignance that remains difficult to cure. Immunotherapy has shown its potential application in a variety of refractory malignancies. Due to the complexity of immune microenvironment of hepatocellular carcinoma, the efficacy of immunotherapy for hepatocellular carcinoma is not as effective as expected. Expression data of hepatocellular carcinoma from the TCGA and ICGC

    更新日期:2020-11-25
  • Increased alpha and beta cell mass during mouse pregnancy is not dependent on transdifferentiation
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-24
    Sandra K Szlapinski; Jamie Bennett; Brenda J Strutt; David J Hill

    Maternal pancreatic beta-cell mass (BCM) increases during pregnancy to compensate for relative insulin resistance. If BCM expansion is suboptimal, gestational diabetes mellitus can develop. Alpha-cell mass (ACM) also changes during pregnancy, but there is a lack of information about α-cell plasticity in pregnancy and whether α- to β-cell transdifferentiation can occur. To investigate this, we used

    更新日期:2020-11-25
  • Resveratrol alleviates alveolar epithelial cell injury induced by hyperoxia by reducing apoptosis and mitochondrial dysfunction
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-20
    Xiaodan Zhu; Fan Wang; Xiaoping Lei; Wenbin Dong

    Bronchopulmonary dysplasia is a severe and long-term pulmonary disease in premature infants. Hyperoxia-induced acute lung injury plays a critical role in bronchopulmonary dysplasia. Resveratrol is a polyphenolic phytoalexin and a natural agonist of Sirtuin 1. Many studies have shown that resveratrol has a protective effect on hyperoxia-induced lung damage, but its specific protective mechanism is still

    更新日期:2020-11-21
  • Fatty acid ethyl esters in meconium: A biomarker of fetal alcohol exposure and effect
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-19
    Charlie T Cheng; Enrique M Ostrea Jr; Joseph NB Alviedo; Felix P Banadera; Ronald L Thomas

    To determine if meconium fatty acid ethyl esters (FAEE) in rat pups is a good biomarker of prenatal exposure and effect to alcohol, three groups of pregnant rats were studied: one control (pair fed) and two treatment groups given 25% alcohol at 2.2 or 5.5 g−1 kg−1 d−1. The pups were delivered on day 20 and, for each dam, were separated into a male and female group. The body, brain, intestines, and

    更新日期:2020-11-19
  • Human-derived osteoblast-like cells and pericyte-like cells induce distinct metastatic phenotypes in primary breast cancer cells
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-19
    Vera Mayo; Annie C Bowles; Laura E Wubker; Ismael Ortiz; Albert M Cordoves; Richard J Cote; Diego Correa; Ashutosh Agarwal

    Approximately 70% of advanced breast cancer patients will develop bone metastases, which accounts for ∼90% of cancer-related mortality. Breast cancer circulating tumor cells (CTCs) establish metastatic tumors in the bone after a close interaction with local bone marrow cells including pericytes and osteoblasts, both related to resident mesenchymal stem/stromal cells (BM-MSCs) progenitors. In vitro

    更新日期:2020-11-19
  • A mini-review on the effects of COVID-19 on younger individuals
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-19
    Madhumitha Manivannan; Manasi P Jogalekar; Muthu Subash Kavitha; Balu Alagar Venmathi Maran; Prakash Gangadaran

    Coronavirus disease 2019 (COVID-19) pandemic has uprooted our lives like never before since its onset in the late December 2019. The world has seen mounting infections and deaths over the past few months despite the unprecedented measures countries are implementing, such as lockdowns, social distancing, mask-wearing, and banning gatherings in large groups. Interestingly, young individuals seem less

    更新日期:2020-11-19
  • Identification of two molecular subtypes of dysregulated immune lncRNAs in ovarian cancer
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-17
    Xiaojun Liu; Jinghai Gao; Jing Wang; Jing Chu; Jiahao You; Zhijun Jin

    Long non-coding RNA (lncRNA) has increasingly been identified as a key regulator in pathologies such as cancer. Multiple platforms were used for comprehensive analysis of ovarian cancer to identify molecular subgroups. However, lncRNA and its role in mapping the ovarian cancer subpopulation are still largely unknown. RNA-sequencing and clinical characteristics of ovarian cancer were acquired from The

    更新日期:2020-11-18
  • Boronate probe-based hydrogen peroxide detection with AlGaN/GaN HEMT sensor
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-17
    Isra Mahaboob; Roger J Reinertsen; Benjamin McEwen; Kasey Hogan; Emma Rocco; J Andres Melendez; Nathaniel C Cady; F Shahedipour-Sandvik

    The results from this study demonstrate the potential of an AlGaN/GaN high electron mobility transistor sensor for the detection of reactive and transient biological molecules such as hydrogen peroxide. A boronate-based fluorescent probe was used with this device to detect the presence of micromolar levels of hydrogen peroxide typically associated with intracellular processes. The real-time electrical

    更新日期:2020-11-18
  • Blockade of OGFr delays the onset and reduces the severity of diabetic ocular surface complications
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-17
    Ian S Zagon; Joseph W Sassani; Indira Purushothaman; Patricia J McLaughlin

    The opioid growth factor (OGF)–OGF receptor (OGFr) pathway is present in the ocular surface and functions to maintain homeostasis of the epithelium. The OGF–OGFr pathway has been reported to be dysregulated in diabetic individuals and animal models, and is reflected in elevations of the inhibitory growth factor, OGF, chemically termed [Met5]-enkephalin. Recently, our laboratory reported elevated levels

    更新日期:2020-11-18
  • Heparin-mediated dimerization of follistatin
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-16
    Ryan G Walker; Chandramohan Kattamuri; Erich J Goebel; Fuming Zhang; Michal Hammel; John A Tainer; Robert J Linhardt; Thomas B Thompson

    Heparin and heparan sulfate (HS) are highly sulfated polysaccharides covalently bound to cell surface proteins, which directly interact with many extracellular proteins, including the transforming growth factor-β (TGFβ) family ligand antagonist, follistatin 288 (FS288). Follistatin neutralizes the TGFβ ligands, myostatin and activin A, by forming a nearly irreversible non-signaling complex by surrounding

    更新日期:2020-11-17
  • Crosstalk between adipocytes and M2 macrophages compensates for osteopenic phenotype in the Lrp5-deficient mice
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-16
    Lisha Li; Xuemin Qiu; Na Zhang; Yan Sun; Yan Wang; Ling Wang

    A loss-of-function mutation in the Lrp5 gene in mice leads to a low bone mass disorder due to the inhibition of the canonical Wnt signaling pathway; however, the role of bone marrow microenvironment in mice with this mutation remains unclear. In this study, we evaluated proliferation and osteogenic potential of mouse osteoblasts using the MTT assay and Alizarin red staining. The levels of alkaline

    更新日期:2020-11-17
  • Corrigendum
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-16

    Pan L, Peng Z, Zhang R, Zhang R, Liang D, Chen H, Tian H. JANEX-1 improves acute pulmonary embolism through VEGF and FAK in pulmonary artery smooth muscle cells. Experimental Biology and Medicine, 245 (15): 1395–1403. DOI: 10.1177/1535370220942474

    更新日期:2020-11-17
  • Pulmonary gene delivery—Realities and possibilities
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-12
    Uday K Baliga; David A Dean

    Delivery of genetic material to tissues in vivo is an important technique used in research settings and is the foundation upon which clinical gene therapy is built. The lung is a prime target for gene delivery due to a host of genetic, acquired, and infectious diseases that manifest themselves there, resulting in many pathologies. However, the in vivo delivery of genetic material to the lung remains

    更新日期:2020-11-13
  • Treatment of osteoporosis with a modified zeolite shows beneficial effects in an osteoporotic rat model and a human clinical trial
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-12
    Sandra Kraljević Pavelić; Vedran Micek; Dragica Bobinac; Edo Bazdulj; Alessandra Gianoncelli; Dalibor Krpan; Marta Žuvić; Sandra Eisenwagen; Peter J Stambrook; Krešimir Pavelić

    The severity of osteoporosis in humans manifests in its high incidence and by its complications that diminish quality of life. A societal consequence of osteoporosis is the substantial burden that it inflicts upon patients and their families. Several bone-modifying drugs have been prescribed to patients with osteoporosis. However, evidence for their anti-fracture efficacy remains inconclusive. To the

    更新日期:2020-11-13
  • Long non-coding RNA IGF2-AS represses breast cancer tumorigenesis by epigenetically regulating IGF2
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-11
    Yanan Zhang; Hanbing Yan; Yan Jiang; Tao Chen; Zhijin Ma; Fei Li; Min Lin; Yanzhi Xu; Xuemei Zhang; Jianming Zhang; Hui He

    Long non-coding RNAs are a kind of endogenous ncRNAs with a length of more than 200 bp. Accumulating evidence suggests that long non-coding RNAs function as pivotal regulators in tumorigenesis and progression. However, their biological roles in breast cancer remain largely unknown. Here, we found that IGF2 antisense RNA (IGF2-AS) was significantly decreased in breast cancer tissues, cell lines, and

    更新日期:2020-11-12
  • Shortened derivatives from native antimicrobial peptide LyeTx I: In vitro and in vivo biological activity assessment
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-11
    Leonardo Lima Fuscaldi; Joaquim Teixeira de Avelar Júnior; Daniel Moreira dos Santos; Daiane Boff; Vívian Louise Soares de Oliveira; Karla Aparecida Guimarães Gusmão Gomes; Rosana de Carvalho Cruz; Patrícia Luciana de Oliveira; Paula Prazeres Magalhães; Patricia Silva Cisalpino; Luiz de Macêdo Farias; Elaine Maria de Souza-Fagundes; Johannes Delp; Marcel Leist; Jarbas Magalhães Resende; Flávio Almeida

    In the continuing search for novel antibiotics, antimicrobial peptides are promising molecules, due to different mechanisms of action compared to classic antibiotics and to their selectivity for interaction with microorganism cells rather than with mammalian cells. Previously, our research group has isolated the antimicrobial peptide LyeTx I from the venom of the spider Lycosa erythrognatha. Here,

    更新日期:2020-11-12
  • Identification of potential lipid biomarkers for active pulmonary tuberculosis using ultra-high-performance liquid chromatography-tandem mass spectrometry
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-11
    Yu-Shuai Han; Jia-Xi Chen; Zhi-Bin Li; Jing Chen; Wen-Jing Yi; Huai Huang; Li-Liang Wei; Ting-Ting Jiang; Ji-Cheng Li

    Early diagnosis of active pulmonary tuberculosis (TB) is the key to controlling the disease. Host lipids are nutrient sources for the metabolism of Mycobacterium tuberculosis. In this research work, we used ultra-high-performance liquid chromatography-tandem mass spectrometry to screen plasma lipids in TB patients, lung cancer patients, community-acquired pneumonia patients, and normal healthy controls

    更新日期:2020-11-12
  • The use of DNA methylation clock in aging research
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-11
    Xi He; Jiaojiao Liu; Bo Liu; Jingshan Shi

    One of the key characteristics of aging is a progressive loss of physiological integrity, which weakens bodily functions and increases the risk of death. A robust biomarker is important for the assessment of biological age, the rate of aging, and a person's health status. DNA methylation clocks, novel biomarkers of aging, are composed of a group of cytosine-phosphate-guanine dinucleotides, the DNA

    更新日期:2020-11-12
  • Animal models of regenerative medicine for biological treatment approaches of degenerative disc diseases
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-11
    Demissew Shenegelegn Mern; Tanja Walsen; Anja Beierfuß; Claudius Thomé

    Degenerative disc disease (DDD) is a painful, chronic and progressive disease, which is characterized by inflammation, structural and biological deterioration of the intervertebral disc (IVD) tissues. DDD is specified as cell-, age-, and genetic-dependent degenerative process that can be accelerated by environmental factors. It is one of the major causes of chronic back pain and disability affecting

    更新日期:2020-11-12
  • Influence of Pseudomonas autoinducer N-3-oxododecanoyl homoserine lactone on human corneal epithelial cells
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-11
    Renjian Hu; Kelan Yuan; Jie Zhou; Yue Zhang; Jiao Zheng; Yingying Zhao; Xiaodan Huang; Xiuming Jin

    The quorum-sensing (QS) signaling-dependent extracellular virulence factors of Pseudomonas aeruginosa can cause infections such as P. aeruginosa keratitis. P. aeruginosa communicates by secreting and sensing small chemical molecules called autoinducers in QS system. The key QS signal molecule, N-3-oxododecanoyl-homoserine lactone (3OC12HSL), can affect the behavior of host cells and initiate immune

    更新日期:2020-11-12
  • Mitophagy promotes the stemness of bone marrow-derived mesenchymal stem cells
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-10
    Xiaorong Feng; Wen Yin; Jialing Wang; Li Feng; Y James Kang

    Previous studies demonstrated that mitochondrial fission arguments the stemness of bone marrow-derived mesenchymal stem cells (BMSCs). Because mitophagy is critical in removing damaged or surplus mitochondrial fragments and maintaining mitochondrial integrity, the present study was undertaken to test the hypothesis that mitophagy is involved in mitochondrial fission-enhanced stemness of BMSCs. Primary

    更新日期:2020-11-12
  • Hypoxia–CXCL6 axis affects arteriolar niche remodeling in acute myeloid leukemia
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-09
    Lijun Li; Jiancheng Man; Li Zhao

    Acute myeloid leukemia (AML) is a malignant clonal disease derived from hematopoietic stem/progenitor cell. Leukemia blasts cause extensive hypoxia of bone marrow (BM), which lead to disorder and remodeling of BM niche, thereby becoming “leukemic niche” to support the development and drug-resistance of AML as well as the maintenance of normal hematopoietic stem cells. In this study, the biological

    更新日期:2020-11-12
  • Recent developments in autophagy-targeted therapies in cancer
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-09
    Manasi P Jogalekar; Anurag Veerabathini; Prakash Gangadaran

    Autophagy plays a crucial role in cellular development and differentiation as well as in the maintenance of homeostasis in healthy cells. Autophagy is well documented in neurodegenerative disorders, aging, and infectious diseases. However, recognizing its significance in cancer has always been challenging due to its tumor-promoting and suppressive attributes. Various modulators targeting key components

    更新日期:2020-11-12
  • Etiological classification and treatment strategies for secondary bile duct dilatation
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-09
    Yunfu Lv; Ning Liu; Hongfei Wu; Zhuori Li

    Secondary intra- and extrahepatic bile duct dilatation is a very common condition that can be caused by several diseases. However, it has been rarely discussed in the specialized literature. Moreover, no distinct etiology can be determined in some cases, which hampers the diagnosis and treatment. Here, we discuss the etiological classification and treatment strategies of secondary intra- and extrahepatic

    更新日期:2020-11-12
  • Detection of severe acute respiratory syndrome coronavirus 2 and influenza viruses based on CRISPR-Cas12a
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-05
    Oraphan Mayuramart; Pattaraporn Nimsamer; Somruthai Rattanaburi; Naphat Chantaravisoot; Kritsada Khongnomnan; Jira Chansaenroj; Jiratchaya Puenpa; Nungruthai Suntronwong; Preeyaporn Vichaiwattana; Yong Poovorawan; Sunchai Payungporn

    Due to the common symptoms of COVID-19, patients are similar to influenza-like illness. Therefore, the detection method would be crucial to discriminate between SARS-CoV-2 and influenza virus-infected patients. In this study, CRISPR-Cas12a-based detection was applied for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza A virus, and influenza B virus which would be

    更新日期:2020-11-06
  • Prenatal indole-3-carbinol administration activates aryl hydrocarbon receptor-responsive genes and attenuates lung injury in a bronchopulmonary dysplasia model
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-04
    Gabriela Guzmán-Navarro; Mario Bermúdez de León; Irene Martín-Estal; Raquel Cuevas-Díaz Durán; Laura Villarreal-Alvarado; Anakaren Vaquera-Vázquez; Tania Cuevas-Cerda; Karina Garza-García; Luis Eduardo Cuervo-Pérez; Álvaro Barbosa-Quintana; José Eduardo Pérez-Saucedo; Víctor J Lara-Díaz; Fabiola Castorena-Torres

    Hyperoxia−hypoxia exposure is a proposed cause of alveolar developmental arrest in bronchopulmonary dysplasia in preterm infants, where mitochondrial reactive oxygen species and oxidative stress vulnerability are increased. The aryl hydrocarbon receptor (AhR) is one of the main activators of the antioxidant enzyme system that protects tissues and systems from damage. The present study aimed to determine

    更新日期:2020-11-06
  • Transcriptomic profiles in Parkinson’s disease
    Exp. Biol. Med. (IF 3.139) Pub Date : 2020-11-04
    Lille Kurvits; Freddy Lättekivi; Ene Reimann; Liis Kadastik-Eerme; Kristjan M Kasterpalu; Sulev Kõks; Pille Taba; Anu Planken

    Transcriptomics in Parkinson’s disease offers insights into the pathogenesis of Parkinson’s disease but obtaining brain tissue has limitations. In order to bypass this issue, we profile and compare differentially expressed genes and enriched pathways (KEGG) in two peripheral tissues (blood and skin) of 12 Parkinson’s disease patients and 12 healthy controls using RNA-sequencing technique and validation

    更新日期:2020-11-06