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Chronic Resveratrol Enhances Endothelium-Dependent Relaxation but Does Not Alter eNOS Levels in Aorta of Spontaneously Hypertensive Rats Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-05 James W. E. Rush, Joe Quadrilatero, Andrew S. Levy, Rebecca J. Ford
Spontaneously hypertensive rats (SHRs) were administered the red wine polyphenol resveratrol in drinking water at 0, 0.448, or 4.48 mg/l (control, low, or high, respectively) for 28 days. The low dosage was chosen to mimic moderate red wine consumption. After the treatment period, thoracic aorta rings were excised for in vitro assessment of vasomotor function. Chronic resveratrol significantly improved
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A BRIEF COMMUNICATION Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-05 Yang Zhou, Youchun Jiang, Y. James Kang
Previous studies have shown that dietary copper deficiency causes cardiac hypertrophy and depression of vascular epithelial growth factor (VEGF) expression in mouse model. Copper replenishment in the diet reverses cardiac hypertrophy and restores VEGF expression. The present study was undertaken to specifically determine the role of VEGF in copper effect on cell hypertrophy. Embryonic rat cardiac H9c2
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Reversal of Elevated Cardiac Expression of TGFβ1 and Endothelin-1 in OLETF Diabetic Rats by Long-Acting Calcium Antagonist Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-05 Subrina Jesmin, Sohel Zaedi, Seiji Maeda, Chishimba N. Mowa, Ichiro Sakuma, Takashi Miyauchi
The effects of calcium channel blockers (CCBs) on complications associated with diabetes mellitus (DM) have been well studied in clinical and basic science investigations. Cardiovascular complications are a common feature of type 2 DM, and insulin resistance is an early clinical manifestation of type 2 DM. CCBs are widely used to treat cardiovascular diseases in patients with DM. In this study, we
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Phosphorylation of Endothelin Converting Enzyme-1 Isoforms: Relevance to Subcellular Localization Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-05 Farahdiba Jafri, Adviye Ergul
Endothelin-converting enzyme (ECE)-1 is a metalloenzyme with four subisoforms, which differ only in their amino-terminal domain. ECE-1a and c are the most common isoforms and are found at the plasma membrane and in the Golgi complex, whereas ECE-1b displays lysosomal localization. We have recently shown that ECE-1a but not ECE-1b also colocalizes with nuclear membrane markers, and that maintenance
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A BRIEF COMMUNICATION Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-05 Agnieszka Walkowska, Leszek Dobrowolski, Elżbieta Kompanowska-Jezierska, Janusz Sadowski
The mechanism of adenosine A1 receptor–induced intrarenal vasoconstriction is unclear; it depends on sodium intake and may be mediated by changing the intrarenal activity of the nitric oxide (NO) and/or cyclooxygenase (COX) pathway of arachidonic acid metabolism. The effects of 2-chloro-N6-cyclopentyl-adenosine (CCPA), a selective A1 receptor agonist, on renal hemodynamics were examined in anesthetized
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Effect of Endothelin Dual Receptor Antagonist on VEGF Levels in Streptozotocin-Induced Diabetic Rat Retina Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-05 Koichi Masuzawa, Subrina Jesmin, Seiji Maeda, Sohel Zaedi, Nobutake Shimojo, Takashi Miyauchi, Katsutoshi Goto
Diabetic retinopathy (DR), one of the most serious causes of blindness, is often associated with the upregulation of vascular endothelial growth factor (VEGF) in retina. Recently, leukocyte adhesion (leukostasis) is blamed for the occlusion of retinal capillary vascularity, which ultimately contributes to the progression of diabetic retinopathy. In addition, intercellular adhesion molecule-1 (ICAM-1)
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Adverse Effects of Rosemary (Rosmarinus officinalis L.) on Reproductive Function in Adult Male Rats Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-05 Mohamad K. Nusier, Hameed N. Bataineh, Haytham M. Daradkah
Ingestion of rosemary ( Rosmarinus officinalis L.) by two groups of adult Sprague-Dawley rats at levels of 250 and 500 mg/kg body wt for 63 days was investigated for its effects on fertility. Body weight and absolute and relative testes weights were not affected, but the average weights of epididymides, ventral prostates, seminal vesicles, and preputial glands decreased significantly. A significant
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Functional Alterations in Macrophages After Hypoxia Selection Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-05 Adriana Degrossoli, Selma Giorgio
Regions of low oxygen tension are common features of inflamed and infected tissues and provide physiologic selective pressure for the expansion of cells with enhanced hypoxia tolerance. The aim of this study was to investigate whether macrophages resistant to death induced by hypoxia were accompanied by functional alterations. A mouse macrophage cell line (J774 cells) was used to obtain subpopulations
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Arrhythmogenic Action of Endothelin-11–31 Through Conversion to Endothelin-11–21 Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-05 An-Jing Ren, Xin Yuan, Li Lin, Jing Xu, Ting Chen, Wei-Zhong Wang, Xiao-Hong Yan, Yong-Wen Qing, Chao-Shu Tang, Wen-Jun Yuan
Endothelin (ET)-11–21 is known to play an important role in the pathogenesis of acute ischemic arrhythmia. In the present study, we attempted to determine whether administration of ET-11–31 would result in arrhythmia in perfused isolated rat hearts. Forty-eight Sprague-Dawley rats weighing ~250–350 g were randomized into 6 groups. Heart was isolated and perfused in a Langendorff mode. The effects of
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Alterations in Gene Expressions Encoding PreproET-1 and NOS in Pulmonary Tissue in Endotoxemic Rats Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-05 Sohel Zaedi, Subrina Jesmin, Seiji Maeda, Nobutake Shimojo, Iwao Yamaguchi, Katsutoshi Goto, Takashi Miyauchi
Septic shock is characterized by hypotension and a hyporeactive response to vasopressor agents. The pathogenesis is due to vascular leaks and an increased synthesis of cytokines and nitric oxide (NO). The present study examined the time-dependent alterations of endothelin-1 (ET-1) and the expression of NO synthase (NOS) in lung tissue in a septic rat model. Normal Sprague-Dawley (SD) rats aged 10 weeks
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Imaging Endothelin ETB Receptors Using [18F]-BQ3020: In Vitro Characterization and Positron Emission Tomography (MicroPET) Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-05 Peter Johnström, James H. F. Rudd, Hugh K. Richards, Tim D. Fryer, John C. Clark, Peter L. Weissberg, John D. Pickard, Anthony P. Davenport
The endothelin (ET) receptor system has been shown to play a role in a number of vascular diseases. We have synthesized 18F-and 11C-labeled radioligands to enable in vivo imaging of the fundamental processes involved in ET receptor pharmacology in normal and diseased tissue using positron emission tomography (PET). One aim is to elucidate the proposed role of the ETB subtype as clearing receptor, removing
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Evaluation of Endothelin-1–Induced Pulmonary Vasoconstriction Following Myocardial Infarction Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-05 Stéphanie Sauvageau, Eric Thorin, Alexandre Caron, Jocelyn Dupuis
Endothelin (ET) levels are elevated in congestive heart failure secondary to myocardial infarction (MI) and correlate well with the severity of pulmonary hypertension (PH), suggesting that the ET peptide could contribute to the pathophysiology of venous PH. Alterations of pulmonary vasoreactivity to ET after MI and the respective roles of the ETA and ETB receptors (ETA-R and ETB-R) have never been
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Topical Application of Plasma Fibronectin in Full-Thickness Skin Wound Healing in Rats Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-05 A-Hon Kwon, Zeyu Qiu, Yutaka Hirao
Fibronectin (Fn) has been shown to play an important role in wound healing because it appears to be the stimulus for migration of fibroblasts and epidermal cells. The purpose of this study was to investigate whether topical application of plasma Fn (pFn) improves healing of full-thickness skin wounds in rats. A round section of full-thickness skin (diameter of approximately 15 mm) was resected in rats
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Curcumin induces ferroptosis and apoptosis in osteosarcoma cells by regulating Nrf2/GPX4 signaling pathway Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-01 Chuanjian Yuan, Rong Fan, Kai Zhu, Yutong Wang, Wenpeng Xie, Yanchen Liang
Curcumin, an antitumor agent, has been shown to inhibit cell growth and metastasis in osteosarcoma. However, there is no evidence of curcumin and its regulation of cell ferroptosis and nuclear factor E2–related factor 2 (Nrf2)/glutathione peroxidase 4 (GPX4) signaling pathways in osteosarcoma. This study aimed to investigate the effects of curcumin on osteosarcoma both in vitro and in vivo. To explore
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RxBERT: Enhancing drug labeling text mining and analysis with AI language modeling Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-01 Leihong Wu, Magnus Gray, Oanh Dang, Joshua Xu, Hong Fang, Weida Tong
The US drug labeling document contains essential information on drug efficacy and safety, making it a crucial regulatory resource for Food and Drug Administration (FDA) drug reviewers. Due to its extensive volume and the presence of free-text, conventional text mining analysis have encountered challenges in processing these data. Recent advances in artificial intelligence (AI) for natural language
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The Janus face of antipsychotics in glial cells: Focus on glioprotection Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-01 Izaviany Schmitz, Amanda da Silva, Larissa Daniele Bobermin, Carlos-Alberto Gonçalves, Johann Steiner, André Quincozes-Santos
Antipsychotics are commonly prescribed to treat several neuropsychiatric disorders, including schizophrenia, mania in bipolar disorder, autism spectrum disorder, delirium, and organic or secondary psychosis, for example, in dementias such as Alzheimer’s disease. There is evidence that typical antipsychotics such as haloperidol are more effective in reducing positive symptoms than negative symptoms
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Whole-genome analysis and evolutionary characterization of cervical and oral human papillomavirus 16 Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-01 Sadia Minhas, Muhammad Kashif, Haseeb Nisar, Muhammad Idrees, Farheen Ansari
High-throughput genome-wide sequencing has revealed high genomic variability of HPV16 in different geographic regions which is the most predominant genotype in human papillomavirus (HPV)–associated malignancies. Analysis of the HPV16 by whole-genome sequence (WGS) is an advanced method for the identification of mutations in the genome. There is limited information about HPV16 diversity in Pakistan
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Deep learning diagnostic performance and visual insights in differentiating benign and malignant thyroid nodules on ultrasound images Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-01 Yujiang Liu, Ying Feng, Linxue Qian, Zhixiang Wang, Xiangdong Hu
This study aims to construct and evaluate a deep learning model, utilizing ultrasound images, to accurately differentiate benign and malignant thyroid nodules. The objective includes visualizing the model’s process for interpretability and comparing its diagnostic precision with a cohort of 80 radiologists. We employed ResNet as the classification backbone for thyroid nodule prediction. The model was
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Inflammation and mitochondria in the pathogenesis of chronic Chagas disease cardiomyopathy Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-01 João Paulo Silva Nunes, Vinicius Moraes de Paiva Roda, Pauline Andrieux, Jorge Kalil, Christophe Chevillard, Edecio Cunha-Neto
Chagas disease (CD), caused by the protozoan parasite Trypanosoma cruzi, is a neglected disease affecting around 6 million people. About 30% of CD patients develop chronic Chagas disease cardiomyopathy (CCC), an inflammatory cardiomyopathy that occurs decades after the initial infection, while most infected patients (60%) remain asymptomatic in the so-called indeterminate form (IF). Death results from
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Aedes-borne orthoflavivirus infections in neotropical primates – Ecology, susceptibility, and pathogenesis Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-01 Paula Rodrigues de Almeida, Matheus Nunes Weber, Luciana Sonne, Fernando Rosado Spilki
Arboviral diseases comprise a group of important infectious diseases imposing a heavy burden to public health in many locations of the world. Orthoflaviviruses are viruses belonging to the genus Orthoflavivirus; this genus includes some of the most relevant arboviruses to human health. Orthoflaviviruses can infect several different hosts, with some species being transmitted in cycles involving birds
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X-linked hypophosphatemia, fibroblast growth factor 23 signaling, and craniosynostosis Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-01 Chelsey Grimbly, Daniel Graf, Leanne M Ward, R Todd Alexander
This review summarizes the current knowledge of fibroblast growth factor 23 signaling in bone and its role in the disease pathology of X-linked hypophosphatemia. Craniosynostosis is an under-recognized complication of X-linked hypophosphatemia. The clinical implications and potential cellular mechanisms invoked by increased fibroblast growth factor 23 signaling causing craniosynostosis are reviewed
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Link between m6A modification and infiltration characterization of tumor microenvironment in lung adenocarcinoma Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-01 Sha Yang, Ke Li, Jiqin Zhang, Jian Liu, Lin Liu, Ying Tan, Chuan Xu
N6-methyladenosine (m6A) RNA methylation plays a pivotal role in immune responses and the onset and advancement of cancer. Nonetheless, the precise impact of m6A modification in lung adenocarcinoma (LUAD) and its associated tumor microenvironment (TME) remains to be fully elucidated. Here, we distinguished distinct m6A modification patterns within two separate LUAD cohorts using a set of 21 m6A regulators
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Quercetin suppresses ovariectomy-induced osteoporosis in rat mandibles by regulating autophagy and the NLRP3 pathway Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-01 Yue Xiong, Cheng-Wei Huang, Chao Shi, Liang Peng, Yu-Ting Cheng, Wei Hong, Jian Liao
With the aging population and the popularity of implant prostheses, an increasing number of postmenopausal osteoporosis (PMOP) patients require implant restorations; however, poor bone condition affects the long-term stability of implant prostheses. This study aimed to investigate the therapeutic effect of quercetin (QR) compared with alendronate (ALN), the primary treatment for PMOP, on mandibular
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Modeling immunity in microphysiological systems Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-01 Brian J Kwee, Xiaoqing Li, Xinh-Xinh Nguyen, Courtney Campagna, Johnny Lam, Kyung E Sung
There is a need for better predictive models of the human immune system to evaluate safety and efficacy of immunomodulatory drugs and biologics for successful product development and regulatory approvals. Current in vitro models, which are often tested in two-dimensional (2D) tissue culture polystyrene, and preclinical animal models fail to fully recapitulate the function and physiology of the human
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Data-driven approach to quantify trust in medical devices using Bayesian networks Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-01 Mini Thomas, Omar Boursalie, Reza Samavi, Thomas E Doyle
Bayesian networks are increasingly used to quantify the uncertainty of subjective and stochastic concepts such as trust. In this article, we propose a data-driven approach to estimate Bayesian parameters in the domain of wearable medical devices. Our approach extracts the probability of a trust factor being in a specific state directly from the devices (e.g. sensor quality). The strength of the relationship
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Integrating artificial intelligence with bioinformatics promotes public health Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-01 Huixiao Hong, William Slikker
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The discovery of subunit-selective GluN1/GluN2B NMDAR antagonist via pharmacophere-based virtual screening Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-01 Jialing Tang, Ju Jin, Zhihong Huang, Faliang An, Caiguo Huang, Wenli Jiang
The incidence and mortality rates of neurodegenerative diseases, such as Alzheimer’s disease and Parkinson’s disease, are gradually increasing worldwide. Numerous studies have demonstrated that N-methyl-D-aspartic acid receptor (NMDAR)-mediated excitotoxicity contributes to neurodegenerative diseases. Ifenprodil, a subtype-selective NMDAR antagonist, showed strong therapeutic potential. However, it
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Arbovirus surveillance in mosquitoes: Historical methods, emerging technologies, and challenges ahead Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-01 Luis Janssen Maia, Cirilo Henrique de Oliveira, Arthur Batista Silva, Pedro Augusto Almeida Souza, Nicolas Felipe Drumm Müller, Jader da Cruz Cardoso, Bergmann Morais Ribeiro, Filipe Vieira Santos de Abreu, Fabrício Souza Campos
Arboviruses cause millions of infections each year; however, only limited options are available for treatment and pharmacological prevention. Mosquitoes are among the most important vectors for the transmission of several pathogens to humans. Despite advances, the sampling, viral detection, and control methods for these insects remain ineffective. Challenges arise with the increase in mosquito populations
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A quantum-based oversampling method for classification of highly imbalanced and overlapped data Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-01 Bei Yang, Guilan Tian, Joseph Luttrell, Ping Gong, Chaoyang Zhang
Data imbalance is a challenging problem in classification tasks, and when combined with class overlapping, it further deteriorates classification performance. However, existing studies have rarely addressed both issues simultaneously. In this article, we propose a novel quantum-based oversampling method (QOSM) to effectively tackle data imbalance and class overlapping, thereby improving classification
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Research of restricted migration evaluation of MDA-MB-231 cells in 2D and 3D co-culture models Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-01 Zhichang Du, Shaohui Yang, Qingzhong Gong, Zhonghua Lin, Guohong Xiao, Shengli Mi
The restricted migration evaluation is conducive to more complex tumor migration research because of the conformity with in vivo tumors. However, the differences between restricted and unrestricted cell migration and the distinction between different evaluation methods have not been systematically studied, hindering related research. In this study, by constructing the restricted environments on chips
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Decision rules for personalized statin treatment prescriptions over multi-objectives Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-01 Pui Ying Yew, Yue Liang, Terrence J Adam, Julian Wolfson, Peter J Tonellato, Chih-Lin Chi
In our previous study, we demonstrated the feasibility of producing a proactive statin prescription strategy – a personalized statin treatment plan (PSTP) – using neural networks with big data. However, its non-transparency limited result interpretations and clinical usability. To improve the transparency of our previous approach with minimal compromise to the maximal statin treatment benefit-to-risk
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Creative and generative artificial intelligence for personalized medicine and healthcare: Hype, reality, or hyperreality? Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-01 Arash Shaban-Nejad, Martin Michalowski, Simone Bianco
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Green synthesized silver nanoparticles for the treatment of diabetes and the related complications of hyperlipidemia and oxidative stress in diabetic rats Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-01 Yousra G El-Baz, Amr Moustafa, Mohamed A Ali, Gaber E El-Desoky, Saikh M Wabaidur, Amjad Iqbal
This study was conducted to compare the impact of cinnamon silver nanoparticles (C-Ag-NPs) and cinnamon aqueous extract (CAE) on the total body weight (TBW), body weight gain (BWG), blood count (BC), fasting blood glucose (FBG), triglycerides (TGs), total cholesterol (TC), low-density (LDL-C) and high-density (HDL-C) lipoprotein cholesterol, liver function enzymes, superoxide dismutase (SOD), catalase
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Single-nucleotide polymorphisms of TLR4 and GAS7 linked to primary open-angle glaucoma among patients of Shenyang, China Exp. Biol. Med. (IF 3.2) Pub Date : 2024-03-01 Jiao Jie, Tengfei Wu
The potential for adverse outcomes and classifications of glaucoma differ among race, country, gender, and family medical history. Nearly, 50 represent candidate genes are considered as potential contributors to the happening for the primary open-angle glaucoma (POAG) since the advent of GWASs. Our investigation is the first to report the Toll-like receptor 4 ( TLR4) and growth arrest-specific 7 (
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Regulation of expression quantitative trait loci by SVA retrotransposons within the major histocompatibility complex. Exp. Biol. Med. (IF 3.2) Pub Date : 2023-11-30 Jerzy K Kulski,Abigail L Pfaff,Luke D Marney,Alexander Fröhlich,Vivien J Bubb,John P Quinn,Sulev Koks
Genomic and transcriptomic studies of expression quantitative trait loci (eQTL) revealed that SINE-VNTR-Alu (SVA) retrotransposon insertion polymorphisms (RIPs) within human genomes markedly affect the co-expression of many coding and noncoding genes by coordinated regulatory processes. This study examined the polymorphic SVA modulation of gene co-expression within the major histocompatibility complex
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Febuxostat ameliorates APAP-induced acute liver injury by activating Keap1/Nrf2 and inhibiting TLR4/NF-κB p65 pathways. Exp. Biol. Med. (IF 3.2) Pub Date : 2023-11-29 Jinhong Tian,Shuaishuai Zhang,Lu Li,Xueman Lin,Yongmei Li,Kunlu Zhao,Fengxin Zheng,Yongjun Chen,Yang Yang,Ting Wu,Jianxin Pang
Excessive acetaminophen (APAP) application is a major cause of drug-induced liver injury (DILI). Febuxostat (Feb), a drug for reducing uric acid (UA) levels, was demonstrated to relieve hepatic inflammation and reverse organ functions. However, the effect of Feb on APAP-induced DILI and its mechanisms have not been fully explored. In this study, Feb (10 mg/kg) was given to mice by gavage 1 h after
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Manipulation of unfolded protein response by zoonotic vaccinia virus strains Guarani P1 and Passatempo. Exp. Biol. Med. (IF 3.2) Pub Date : 2023-11-29 Karine Lima Lourenço,Thiago Lima Leão,Cid Oliveira de Queiroz,Ângela Vieira Serufo,Flávio Guimarães da Fonseca
The replicative success of vaccinia virus (VACV) depends on its ability to subvert host functions. Poxviruses multiplication and maturation are closely associated with the endoplasmic reticulum (ER) and its membranes. This organelle responds to disturbances caused by the accumulation of misfolded proteins, leading to processing of these proteins or even programmed cell death through the unfolded protein
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Penicillin-binding protein (PBP) inhibitor development: A 10-year chemical perspective. Exp. Biol. Med. (IF 3.2) Pub Date : 2023-11-29 Ariane F Bertonha,Caio C L Silva,Karina T Shirakawa,Daniel M Trindade,Andréa Dessen
Bacterial cell wall formation is essential for cellular survival and morphogenesis. The peptidoglycan (PG), a heteropolymer that surrounds the bacterial membrane, is a key component of the cell wall, and its multistep biosynthetic process is an attractive antibacterial development target. Penicillin-binding proteins (PBPs) are responsible for cross-linking PG stem peptides, and their central role in
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Transcriptomic profiling of cerebrospinal fluid identifies ALS pathway enrichment and RNA biomarkers in MND individuals. Exp. Biol. Med. (IF 3.2) Pub Date : 2023-11-24 Alexander Fröhlich,Abigail L Pfaff,Vivien J Bubb,John P Quinn,Sulev Koks
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder and the most common form of motor neurone disease (MND) which is characterized by the damage and death of motor neurons in the brain and spinal cord of affected individuals. Due to the heterogeneity of the disease, a better understanding of the interaction between genetics and biochemistry with the identification of biomarkers
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Developing a SARS-CoV-2 main protease binding prediction random forest model for drug repurposing for COVID-19 treatment. Exp. Biol. Med. (IF 3.2) Pub Date : 2023-11-24 Jie Liu,Liang Xu,Wenjing Guo,Zoe Li,Md Kamrul Hasan Khan,Weigong Ge,Tucker A Patterson,Huixiao Hong
The coronavirus disease 2019 (COVID-19) global pandemic resulted in millions of people becoming infected with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus and close to seven million deaths worldwide. It is essential to further explore and design effective COVID-19 treatment drugs that target the main protease of SARS-CoV-2, a major target for COVID-19 drugs. In this study
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The intestinal microbiota as mediators between dietary contaminants and host health. Exp. Biol. Med. (IF 3.2) Pub Date : 2023-11-24 Amon Cox,Zach Bomstein,Arul Jayaraman,Clinton Allred
The gut microbiota sit at an important interface between the host and the environment, and are exposed to a multitude of nutritive and non-nutritive substances. These microbiota are critical to maintaining host health, but their supportive roles may be compromised in response to endogenous compounds. Numerous non-nutritive substances are introduced through contaminated foods, with three common groups
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Surface fibrils on the particles of nucleocytoviruses: A review. Exp. Biol. Med. (IF 3.2) Pub Date : 2023-11-13 Isabella Luiza Martins de Aquino,Matheus Gomes Barcelos,Talita Bastos Machado,Mateus Sá Magalhães Serafim,Jônatas Santos Abrahão
The capsid has a central role in viruses' life cycle. Although one of its major functions is to protect the viral genome, the capsid may be composed of elements that, at some point, promote interaction with host cells and trigger infection. Considering the scenario of multiple origins of viruses along the viral evolution, a substantial number of capsid shapes, sizes, and symmetries have been described
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Extracellular matrix metalloproteinases inducer gene polymorphism and reduced serum matrix metalloprotease-2 activity in preeclampsia patients. Exp. Biol. Med. (IF 3.2) Pub Date : 2023-11-08 Daniel Amakye,Priscilla O Gyan,Sheila Santa,Nii Ayite Aryee,Kwame Adu-Bonsaffoh,Osbourne Quaye,Emmanuel Ayitey Tagoe
Preeclampsia increases the risk of pregnancy-related complications, nevertheless a successful spiral vessel remodeling, and trophoblast invasion reduces disorders of pregnancy. Matrix metalloproteinase-2 (MMP-2) clears the path for trophoblast invasion, and activation of MMP-2 largely depends on extracellular matrix metalloproteinases inducer (EMMPRIN) protein. This study aimed to investigate EMMPRIN
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Structural and molecular biology of Sabiá virus. Exp. Biol. Med. (IF 3.2) Pub Date : 2023-11-08 Eduardo Hs Bezerra,Talita D Melo-Hanchuk,Rafael Elias Marques
Brazilian mammarenavirus, or Sabiá virus (SABV), is a New World (NW) arenavirus associated with fulminant hemorrhagic disease in humans and the sole biosafety level 4 microorganism ever isolated in Brazil. Since the isolation of SABV in the 1990s, studies on viral biology have been scarce, with no available countermeasures against SABV infection or disease. Here we provide a comprehensive review of
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Primary cilium-mediated mechanotransduction in cartilage chondrocytes. Exp. Biol. Med. (IF 3.2) Pub Date : 2023-10-28 Yang Zhang,Godfred K Tawiah,Xiaoan Wu,Yanjun Zhang,Xiaohu Wang,Xiaochun Wei,Xiaohong Qiao,Quanyou Zhang
Osteoarthritis (OA) is one of the most prevalent joint disorders associated with the degradation of articular cartilage and an abnormal mechanical microenvironment. Mechanical stimuli, including compression, shear stress, stretching strain, osmotic challenge, and the physical properties of the matrix microenvironment, play pivotal roles in the tissue homeostasis of articular cartilage. The primary
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Analysis, validation, and discussion of key genes in placenta of patients with gestational diabetes mellitus. Exp. Biol. Med. (IF 3.2) Pub Date : 2023-10-24 Yi Jiang,Yuanyuan Du,Rui Su,Lijie Wei,Peng Gao,Jingyi Zhang,Xuan Zhou,Shenglan Zhu,Huiting Zhang,Yuting Chen,Chenyun Fang,Shaoshuai Wang,Jun Yu,Wencheng Ding,Ling Feng
Gestational diabetes mellitus (GDM) is a common complication during pregnancy, which can have harmful health consequences for both the mother and the fetus. Given the placenta's crucial role as an endocrine organ during pregnancy, exploring and validating key genes in the placenta hold significant potential in the realm of GDM prevention and treatment. In this study, differentially expressed genes
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Connective tissue growth factor: Role in trabecular meshwork remodeling and intraocular pressure lowering. Exp. Biol. Med. (IF 3.2) Pub Date : 2023-10-24 Mohammad Daniel Shafiq Hassan,Norhafiza Razali,Amy Suzana Abu Bakar,Noor Fahitah Abu Hanipah,Renu Agarwal
Connective tissue growth factor (CTGF) is a distinct signaling molecule modulating many physiological and pathophysiological processes. This protein is upregulated in numerous fibrotic diseases that involve extracellular matrix (ECM) remodeling. It mediates the downstream effects of transforming growth factor beta (TGF-β) and is regulated via TGF-β SMAD-dependent and SMAD-independent signaling routes
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Leonurine pretreatment protects the heart from myocardial ischemia-reperfusion injury. Exp. Biol. Med. (IF 3.2) Pub Date : 2023-10-24 Huiping Lu,Jingru Gong,Tongtong Zhang,Zhe Jiang,Wenmin Dong,Jing Dai,Fenfen Ma
Myocardial ischemia-reperfusion (I/R), an important complication of reperfusion therapy for myocardial infarction, is characterized by hyperactive oxidative stress and inflammatory response. Leonurine (4-guanidino-n-butyl syringate, SCM-198), an alkaloid extracted from Herbaleonuri, was previously found to be highly cardioprotective both in vitro and in vivo. Our current study aimed to investigate
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Gentiopicroside alleviates acute myocardial infarction injury in rats by disrupting Nrf2/NLRP3 signaling. Exp. Biol. Med. (IF 3.2) Pub Date : 2023-10-18 Fei Li,Hongxiang Zhu,Zijuan Chang,Ying Li
The objective of the present investigation was to assess the protective impact of gentiopicroside (GPS) on acute myocardial infarction (AMI) through the modulation of NF-E2-related factor 2 (Nrf2)/nucleotide-binding oligomerization domain, leucine-rich repeat, and pyrin domain-containing 3 (NLRP3) signaling. H9c2 cells were subjected to varying concentrations of GPS, and subsequently, the cells and
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Proteomics analysis of carotid body tumor revealed potential mechanisms and molecular differences among Shamblin classifications. Exp. Biol. Med. (IF 3.2) Pub Date : 2023-10-16 Yanze Lv,Guangchao Gu,Rong Zeng,Zhili Liu,Jianqiang Wu,Yuehong Zheng
Carotid body tumors (CBTs) are a rare type of paraganglioma, and surgical resection is the only effective treatment. Because of the proximity of CBTs to the carotid artery, jugular vein, and cranial nerve, surgery is extremely difficult, with high risks of hemorrhage and neurovascular injury. The Shamblin classification is used for CBT clinical evaluation; however, molecular mechanisms underlying classification
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Respiratory viral infection and resolution of inflammation: Roles for specialized pro-resolving mediators. Exp. Biol. Med. (IF 3.2) Pub Date : 2023-10-14 Luciana P Tavares,Julie Nijmeh,Bruce D Levy
Respiratory viral infections with influenza A virus (IAV) or respiratory syncytial virus (RSV) pose a significant threat to public health due to excess morbidity and mortality. Dysregulated and excessive inflammatory responses are major underlying causes of viral pneumonia severity and morbidity, including aberrant host immune responses and increased risk for secondary bacterial infections. Currently
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Dietary supplementation with L-leucine reduces nitric oxide synthesis by endothelial cells of rats. Exp. Biol. Med. (IF 3.2) Pub Date : 2023-10-14 Carmen D Tekwe,Yuanyuan Luan,Cynthia J Meininger,Fuller W Bazer,Guoyao Wu
This study tested the hypothesis that elevated L-leucine concentrations in plasma reduce nitric oxide (NO) synthesis by endothelial cells (ECs) and affect adiposity in obese rats. Beginning at four weeks of age, male Sprague-Dawley rats were fed a casein-based low-fat (LF) or high-fat (HF) diet for 15 weeks. Thereafter, rats in the LF and HF groups were assigned randomly into one of two subgroups (n
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Quercetin supplementation attenuates airway hyperreactivity and restores airway relaxation in rat pups exposed to hyperoxia. Exp. Biol. Med. (IF 3.2) Pub Date : 2023-10-14 Islam Kryeziu,Shkëlzen Reçica,Qëndrim Thaçi,Fisnik Kurshumliu,Nikola Hadzi-Petrushev,Mimoza Basholli-Salihu,Mitko Mladenov,Ramadan B Sopi
Hyperoxia exposure of immature lungs contributes to lung injury and airway hyperreactivity. Up to now, treatments of airway hyperreactivity induced by hyperoxia exposure have been ineffective. The aim of this study was to investigate the effects of quercetin on hyperoxia-induced airway hyperreactivity, impaired relaxation, and lung inflammation. Newborn rats were exposed to hyperoxia (FiO2 > 95%) or
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Synthesis of glycine from 4-hydroxyproline in tissues of neonatal pigs with intrauterine growth restriction. Exp. Biol. Med. (IF 3.2) Pub Date : 2023-10-14 Shengdi Hu,Wenliang He,Fuller W Bazer,Gregory A Johnson,Guoyao Wu
This study tested the hypothesis that the synthesis of glycine from 4-hydroxyproline (an abundant amino acid in milk and neonatal blood) was impaired in tissues of piglets with intrauterine growth restriction (IUGR), thereby contributing to a severe glycine deficiency in these compromised neonates. At 0, 7, 14, and 21 days of age, IUGR piglets were euthanized, and tissues (liver, small intestine, kidney
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Parametric optimization of culture chamber for cell mechanobiology research. Exp. Biol. Med. (IF 3.2) Pub Date : 2023-10-14 Xutong Guo,Ziqi Wang,Lilan Gao,Chunqiu Zhang
Mechanical signals influence the morphology, function, differentiation, proliferation, and growth of cells. Due to the small size of cells, it is essential to analyze their mechanobiological responses with an in vitro mechanical loading device. Cells are cultured on an elastic silicone membrane substrate, and mechanical signals are transmitted to the cells by the substrate applying mechanical loads
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TRIM29 promotes the progression of colorectal cancer by suppressing EZH2 degradation. Exp. Biol. Med. (IF 3.2) Pub Date : 2023-10-14 Yuanjian Chen,Jun Ma,Mingming Zhang
Colorectal cancer (CRC) is commonly diagnosed at the advanced stage and has a high mortality rate. Tripartite Motif Containing 29 (TRIM29) is an oncogene in numerous malignancies including CRC. However, the molecular mechanism of TRIM29 is largely unknown. In this study, we investigated the biological functions of TRIM29 and the underlying mechanisms. The expression of TRIM29 and Enhancer of Zeste
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CRISPR/SaCas9-based gene editing rescues photoreceptor degeneration throughout a rhodopsin-associated autosomal dominant retinitis pigmentosa mouse model. Exp. Biol. Med. (IF 3.2) Pub Date : 2023-10-14 Wei Du,Jiarui Li,Xin Tang,Wenzhen Yu,Mingwei Zhao
Rhodopsin (Rho) gene mutation was considered the highest prevalent mutation in autosomal dominant retinitis pigmentosa (ADRP); however, effective therapeutics for ADRP have not been developed. The process of gene editing via the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system offers the potentiality to provide cures for dominantly inherited disorders. Herein, we generated