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An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7 SLAS Technol. (IF 2.174) Pub Date : 2021-01-12 Cheng Liu; Shuiqin Fang; Yachen Tian; Youxue Wu; Meijiao Wu; Zheng Wang; Dongpo Xu; Dongjun Hou; Qing Liu
Escherichia coli O157:H7 (E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent
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Rapid and Sensitive Liquid Chromatographic Method for Determination of Anastrozole in Different Polymer–Lipid Hybrid Nanoparticles SLAS Technol. (IF 2.174) Pub Date : 2021-01-12 Dilshad Ahmad; Faisal A. Al Meshaiti; Yazeed K. Al Anazi; Osama Al Owassil; Alaa Eldeen B. Yassin
Anastrozole, an aromatase inhibitor drug, is used for the treatment of breast cancer in pre- and postmenopausal women. Anastrozole’s incorporation into nanoparticulate carriers would enhance its therapeutic performance. To perceive the exact loaded amount of drug in nanocarriers, a valid analytical method is required. The reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed
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Application of Artificial Intelligence to Address Issues Related to the COVID-19 Virus SLAS Technol. (IF 2.174) Pub Date : 2021-01-04 M. Senthilraja
Artificial intelligence (AI) plays a major role in addressing novel coronavirus 2019 (COVID-19)-related issues and is also used in computer-aided synthesis planning (CASP). AI, including machine learning, is used by artificial neural networks such as deep neural networks and recurrent networks. AI has been used in activity predictions like physicochemical properties. Machine learning in de novo design
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Development of a Gold Nanoparticle-Based Colorimetric Sensor for Water for Injection At-Line Impurity Testing SLAS Technol. (IF 2.174) Pub Date : 2020-12-29 Saskia Reichelt; Elke Boschke; Olena Reinhardt; Thomas Walther; Felix Lenk
The best-known rapid test using gold nanoparticles (AuNPs) is the human chorionic gonadotropin pregnancy test. AuNPs are a powerful tool in point-of-care testing because of their flexibility, modifiability, and visibility. Here, we report a method to detect impurities for at-line process control in water-for-injection (WFI) manufacturing through the example of endotoxins. If a distinct concentration
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Therapeutic Potential of Reactive Oxygen Species: State of the Art and Recent Advances SLAS Technol. (IF 2.174) Pub Date : 2020-12-21 Valeria Graceffa
In the last decade, several studies have proven that when at low concentration reactive oxygen species (ROS) show an adaptive beneficial effect and posited the idea that they can be utilized as inexpensive and convenient inducers of tissue regeneration. On the other hand, the recent discovery that cancer cells are more sensitive to oxidative damage paved the way for their use in the selective killing
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epiTracker: A Framework for Highly Reliable Particle Tracking for the Quantitative Analysis of Fish Movements in Tanks SLAS Technol. (IF 2.174) Pub Date : 2020-12-21 Roman Bruch; Paul M. Scheikl; Ralf Mikut; Felix Loosli; Markus Reischl
Behavioral analysis of moving animals relies on a faithful recording and track analysis to extract relevant parameters of movement. To study group behavior and social interactions, often simultaneous analyses of individuals are required. To detect social interactions, for example to identify the leader of a group as opposed to followers, one needs an error-free segmentation of individual tracks throughout
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Creating an Affordable, User-Friendly Electronic Inventory System for Lab Samples SLAS Technol. (IF 2.174) Pub Date : 2020-12-11 Kate Dennert; Logan Friedrich; Rajeev Kumar
Sharing physical laboratory space optimizes resource use among research departments, improves collaboration, and reduces costs. Universal inventory management is essential to successful space sharing, item tracking and availability, and data mining; therefore, our lab was interested in a tool for these purposes. Different research groups have differing needs, priorities, and considerations. We investigated
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Automating Human Induced Pluripotent Stem Cell Culture and Differentiation of iPSC-Derived Retinal Pigment Epithelium for Personalized Drug Testing SLAS Technol. (IF 2.174) Pub Date : 2020-12-09 Vincent Truong; Kevin Viken; Zhaohui Geng; Samantha Barkan; Blake Johnson; Mara C. Ebeling; Sandra R. Montezuma; Deborah A. Ferrington; James R. Dutton
Derivation and differentiation of human induced pluripotent stem cells (hiPSCs) provide the opportunity to generate medically important cell types from individual patients and patient populations for research and the development of potential cell therapies. This technology allows disease modeling and drug screening to be carried out using diverse population cohorts and with more relevant cell phenotypes
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A Variable Scheduling Maintenance Culture Platform for Mammalian Cells SLAS Technol. (IF 2.174) Pub Date : 2020-12-03 Koji Ochiai; Naohiro Motozawa; Motoki Terada; Takaaki Horinouchi; Tomohiro Masuda; Taku Kudo; Motohisa Kamei; Akitaka Tsujikawa; Kenji Matsukuma; Tohru Natsume; Genki N. Kanda; Masayo Takahashi; Koichi Takahashi
Cell culturing is a basic experimental technique in cell biology and medical science. However, culturing high-quality cells with a high degree of reproducibility relies heavily on expert skills and tacit knowledge, and it is not straightforward to scale the production process due to the education bottleneck. Although many automated culture systems have been developed and a few have succeeded in mass
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Evaluation of the Use of Cold Plasma for Microtiter Plate Cleaning to Reduce Plastic Biohazard Waste SLAS Technol. (IF 2.174) Pub Date : 2020-11-30 Helen Plant; Paul Hensley; Geoffrey Holdgate; Paul Jonsen; Mark Wigglesworth
Plastic pollution is the accumulation of plastic objects in the Earth’s environment and is a global problem of increasing importance. The laboratory and health care industries contribute to this problem by the widely accepted single use of plastics, including microtiter plates used for compound testing. At AstraZeneca, we predict the use of more than 45,000 384-well and more than 11,000 1536-well microtiter
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Advances in Technology to Address COVID-19 SLAS Technol. (IF 2.174) Pub Date : 2020-11-20 Edward Kai-Hua Chow; Pak Kin Wong; Xianting Ding
This year has seen an unprecedented worldwide pandemic that has been brought on by the rise of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which results in COVID-19 (coronavirus 2019) infections. COVID-19 has impacted every aspect of our lives and has required the world to rapidly mobilize to address all aspects of diagnosis and treatment of this disease. COVID-19 has brought to light
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Adapting a Low-Cost and Open-Source Commercial Pipetting Robot for Nanoliter Liquid Handling SLAS Technol. (IF 2.174) Pub Date : 2020-11-19 E. Enoch A. W. Councill; Nathanial B. Axtell; Thy Truong; Yiran Liang; Adam L. Aposhian; Kei G. I. Webber; Ying Zhu; Yongzheng Cong; Richard H. Carson; Ryan T. Kelly
Low-volume liquid handling capabilities in bioanalytical workflows can dramatically improve sample processing efficiency and reduce reagent costs, yet many commercial nanoliter liquid handlers cost tens of thousands of dollars or more. We have successfully adapted a low-cost and open-source commercial pipetting robot, the Opentrons OT-1, to accurately aspirate and dispense nanoliter volumes. Based
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Highly Versatile Cloud-Based Automation Solution for the Remote Design and Execution of Experiment Protocols during the COVID-19 Pandemic SLAS Technol. (IF 2.174) Pub Date : 2020-11-19 Piero Zucchelli; Giorgio Horak; Nigel Skinner
There is an urgent need to accelerate the development and validation of both diagnostics and vaccines for COVID-19. These priorities are challenging both public and private sector research groups around the world and have shone a spotlight on both existing bottlenecks in the research workflows involved as well as on the implications of having to do much of this work remotely because of enforced social
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Metabolomic Signatures in Pediatric Crohn’s Disease Patients with Mild or Quiescent Disease Treated with Partial Enteral Nutrition: A Feasibility Study SLAS Technol. (IF 2.174) Pub Date : 2020-11-18 Jair Gonzalez Marques; Engy Shokry; Klara Frivolt; Katharina Julia Werkstetter; Annecarin Brückner; Tobias Schwerd; Sibylle Koletzko; Berthold Koletzko
Little is known about the metabolic response of pediatric Crohn’s disease (CD) patients to partial enteral nutrition (PEN) therapy and the impact of disease activity and inflammation. We analyzed plasma samples from a nonrandomized controlled intervention study investigating the effect of partial enteral nutrition (PEN) on bone health and growth throughout one year with untargeted metabolomics using
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Acoustic Ejection/Full-Scan Mass Spectrometry Analysis for High-Throughput Compound Quality Control SLAS Technol. (IF 2.174) Pub Date : 2020-11-16 Jun Zhang; Yong Zhang; Chang Liu; Tom Covey; Julia Nielsen; Shu Li; Harold Weller; Wilson Shou
High-throughput analysis of compound dissolved in DMSO and arrayed in multiwell plates for quality control (QC) purposes has widespread utility in drug discovery, ranging from the QC of assay-ready plates dispatched by compound management, to compound integrity check in the screening collection, to reaction monitoring of chemical syntheses in microtiter plates. Due to the large number of samples (thousands
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An Emerging Fluorescence-Based Technique for Quantification and Protein Profiling of Extracellular Vesicles SLAS Technol. (IF 2.174) Pub Date : 2020-11-13 Mehdi Dehghani; Rebecca K. Montange; Michael W. Olszowy; David Pollard
Robust and well-established techniques for the quantification and characterization of extracellular vesicles (EVs) are a crucial need for the utilization of EVs as potential diagnostic and therapeutic tools. Current bulk analysis techniques such as proteomics and Western blot suffer from low resolution in the detection of small changes in target marker expression levels, exemplified by the heterogeneity
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Integrating Mobile Robots into Automated Laboratory Processes: A Suitable Workflow Management System SLAS Technol. (IF 2.174) Pub Date : 2020-11-12 Kerstin Thurow; Xiangyu Gu; Bernd Göde; Thomas Roddelkopf; Heidi Fleischer; Norbert Stoll; Sebastian Neubert
The general trend of automation is currently increasing in life science laboratories. The samples to be examined show a high diversity in their structures and composition as well as the determination methods. Complex automation lines such as those used in classic industrial automation are not a suitable solution with respect to the required flexibility of the systems due to changing application requirements
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Development of an Enhanced-Throughput Radial Cell Migration Device SLAS Technol. (IF 2.174) Pub Date : 2020-11-12 C. Ryan Oliver; Andrew C. Little; Trisha M. Westerhof; Pragathi Pathanjeli; Joel A. Yates; Sofia D. Merajver
It is often desirable to evaluate the ability of cells to move in an unrestricted manner in multiple directions without chemical gradients. By combining the standard radial migration assay with injection-molded gaskets and a rigid fixture, we have developed a highly reliable and sensitive method for observing and measuring radial cell migration. This method is adapted for use on high-throughput automated
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Recent Developments in Bacterial Chemotaxis Analysis Based on the Microfluidic System SLAS Technol. (IF 2.174) Pub Date : 2020-11-04 Heon-Ho Jeong
Bacterial motility in response to chemicals, also called bacterial chemotaxis, is a critical ability to search for the optimal environment to ensure the survival of bacterial species. Recent advances in microbiology have allowed the engineering of bacterial chemotactic properties. Conventional methods for characterizing bacterial motility are not able to fully monitor chemotactic behavior. Developments
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Automated Device for Uncapping Multiple-Size Bioanalytical Sample Tubes Designed to Reduce Technician Strain and Increase Productivity SLAS Technol. (IF 2.174) Pub Date : 2020-10-22 Jacob W. Jaeger; Scottland C. Adkins; Samuel C. Perez-Tamayo; Katelyn E. Werth; Gregory Hansen; Amit J. Nimunkar; Robert G. Radwin
Technicians in a commercial laboratory manually uncap up to 700 sample tubes daily in preparation for bioanalytical testing. Manually twisting off sample tube caps not only is a time-consuming task, but also poses increased risk for muscle fatigue and repetitive-motion injuries. An automated device capable of uncapping sample tubes at a rate faster than the current workflow would be valuable for minimizing
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Artificial Intelligence (AI) to the Rescue: Deploying Machine Learning to Bridge the Biorelevance Gap in Antioxidant Assays SLAS Technol. (IF 2.174) Pub Date : 2020-10-15 Sunday Olakunle Idowu; Amos Akintayo Fatokun
Oxidative stress induced by excessive levels of reactive oxygen species (ROS) underlies several diseases. Therapeutic strategies to combat oxidative damage are, therefore, a subject of intense scientific investigation to prevent and treat such diseases, with the use of phytochemical antioxidants, especially polyphenols, being a major part. Polyphenols, however, exhibit structural diversity that determines
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Point-of-Need Diagnostics for Foodborne Pathogen Screening SLAS Technol. (IF 2.174) Pub Date : 2020-10-05 Veronica R. Campbell; Mariam S. Carson; Amelia Lao; Kajal Maran; Eric J. Yang; Daniel T. Kamei
Foodborne illness is a major public health issue that results in millions of global infections annually. The burden of such illness sits mostly with developing countries, as access to advanced laboratory equipment and skilled lab technicians, as well as consistent power sources, is limited and expensive. Current gold standards in foodborne pathogen screening involve labor-intensive sample enrichment
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Detection Methods of COVID-19 SLAS Technol. (IF 2.174) Pub Date : 2020-09-30 Amira Echtioui; Wassim Zouch; Mohamed Ghorbel; Chokri Mhiri; Habib Hamam
Since being first detected in China, coronavirus disease 2019 (COVID-19) has spread rapidly across the world, triggering a global pandemic with no viable cure in sight. As a result, national responses have focused on the effective minimization of the spread. Border control measures and travel restrictions have been implemented in a number of countries to limit the import and export of the virus. The
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Detection of COVID-19 from Chest X-Ray Images Using Convolutional Neural Networks. SLAS Technol. (IF 2.174) Pub Date : 2020-09-18 Boran Sekeroglu,Ilker Ozsahin
The detection of severe acute respiratory syndrome coronavirus 2 (SARS CoV-2), which is responsible for coronavirus disease 2019 (COVID-19), using chest X-ray images has life-saving importance for both patients and doctors. In addition, in countries that are unable to purchase laboratory kits for testing, this becomes even more vital. In this study, we aimed to present the use of deep learning for
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Artificial Intelligence Effecting a Paradigm Shift in Drug Development. SLAS Technol. (IF 2.174) Pub Date : 2020-09-17 Masturah Bte Mohd Abdul Rashid
The inverse relationship between the cost of drug development and the successful integration of drugs into the market has resulted in the need for innovative solutions to overcome this burgeoning problem. This problem could be attributed to several factors, including the premature termination of clinical trials, regulatory factors, or decisions made in the earlier drug development processes. The introduction
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Performance Comparison of Massively Parallel Sequencing (MPS) Instruments Using Single-Nucleotide Polymorphism (SNP) Panels for Ancestry. SLAS Technol. (IF 2.174) Pub Date : 2020-09-11 Ashley M Cooley,Kelly A Meiklejohn,Natalie Damaso,James M Robertson,Tracey Dawson Cruz
Thermo Fisher Scientific released the Precision ID Ancestry Panel, a 165-single-nucleotide polymorphism (SNP) panel for ancestry prediction that was initially compatible with the manufacturer’s massively parallel sequencer, the Ion Torrent Personal Genome Machine (PGM). The semiautomated workflow using the panel with the PGM involved several time-consuming manual steps across three instruments, including
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AmbuBox: A Fast-Deployable Low-Cost Ventilator for COVID-19 Emergent Care. SLAS Technol. (IF 2.174) Pub Date : 2020-09-03 Zecong Fang,Andrew I Li,Hongcheng Wang,Ruoyu Zhang,Xiyan Mai,Tingrui Pan
We present a low-cost clinically viable ventilator design, AmbuBox, using a controllable pneumatic enclosure and standard manual resuscitators that are readily available (AmbuBag), which can be rapidly deployed during pandemic and mass-casualty events with a minimal set of components to manufacture and assemble. The AmbuBox is designed to address the existing challenges presented in the existing low-cost
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Advances in Viral Diagnostic Technologies for Combating COVID-19 and Future Pandemics. SLAS Technol. (IF 2.174) Pub Date : 2020-08-24 Ninghao Zhu,Pak Kin Wong
The emergence of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) threatens the health of the global population and challenges our preparedness for pandemic threats. Previous outbreaks of coronaviruses and other viruses have suggested the importance of diagnostic technologies in fighting viral outbreaks. Nucleic acid detection techniques are
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Sensitive and Specific Detection of SARS-CoV-2 Antibodies Using a High-Throughput, Fully Automated Liquid-Handling Robotic System. SLAS Technol. (IF 2.174) Pub Date : 2020-08-20 Donna Grace Karp,Deanne Cuda,Devangkumar Tandel,Kenneth Danh,Peter V Robinson,David Seftel,Honglin Tian,Mark Pandori,Kevin W P Miller,Cheng-T Tsai
As of July 22, 2020, more than 14.7 million infections of SARS-CoV-2, the virus responsible for Coronavirus Disease 2019 (COVID-19), have been confirmed globally. Serological assays are essential for community screening, assessing infection prevalence, aiding identification of infected patients, and enacting appropriate treatment and quarantine protocols in the battle against this rapidly expanding
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The Virological, Immunological, and Imaging Approaches for COVID-19 Diagnosis and Research. SLAS Technol. (IF 2.174) Pub Date : 2020-08-18 An Sen Tan,Sanjna Nilesh Nerurkar,Wei Chang Colin Tan,Denise Goh,Chi Peng Timothy Lai,Joe Poh Sheng Yeong
In 2019, a novel coronavirus (SARS-CoV-2) was found to cause a highly contagious disease characterized by pneumonia. The disease (COVID-19) quickly spread around the globe, escalating to a global pandemic. In this review, we discuss the virological, immunological, and imaging approaches harnessed for COVID-19 diagnosis and research. COVID-19 shares many clinical characteristics with other respiratory
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Miniaturized Drug Sensitivity and Resistance Test on Patient-Derived Cells Using Droplet-Microarray. SLAS Technol. (IF 2.174) Pub Date : 2020-08-13 Anna A Popova,Sascha Dietrich,Wolfgang Huber,Markus Reischl,Ravindra Peravali,Pavel A Levkin
Testing the sensitivity of patient-derived tumor cells ex vivo can potentially help determining the appropriate treatment for each patient and spot the development of resistance to a given therapy. The number of cells obtainable from a biopsy is, however, often insufficient for performing ex vivo tests in conventional microtiter plates. Here, we introduce a novel Droplet-Microarray platform based on
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Novel Self-Assembled Polycaprolactone-Lipid Hybrid Nanoparticles Enhance the Antibacterial Activity of Ciprofloxacin. SLAS Technol. (IF 2.174) Pub Date : 2020-07-31 Mustafa E Omer,Majed Halwani,Rayan M Alenazi,Omar Alharbi,Shokran Aljihani,Salam Massadeh,Majed Al Ghoribi,Manal Al Aamery,Alaa Eldeen Yassin
Ciprofloxacin (CIP), a widely used antibiotic, is a poor biopharmaceutical resulting in low bioavailability. We optimized a CIP polymer–lipid hybrid nanoparticle (CIP-PLN) delivery system to enhance its biopharmaceutical attributes and the overall therapeutic performance. CIP-PLN formulations were prepared by a direct emulsification–solvent–evaporation method. Varying the type and ratio of lipid was
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Xcellerate Investigator Portal: A New Web-Based Tool for Online Delivery of Central Laboratory Data, Reports, and Communications to Clinical Sites. SLAS Technol. (IF 2.174) Pub Date : 2020-07-29 Louis Norton,Joseph Ciervo,Victor S Lobanov,Dimitris K Agrafiotis
Covance Drug Development produces more than 55 million test results via its central laboratory services, requiring the delivery of more than 10 million reports annually to investigators at 35,000 sites in 89 countries. Historically, most of these data were delivered via fax or electronic data transfers in delimited text or SAS transport file format. Here, we present a new web portal that allows secure
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Carbohydrate Structure Analysis: Methods and Applications. SLAS Technol. (IF 2.174) Pub Date : 2020-07-20 Christian Heiss,Parastoo Azadi
The fact that practically all living cells are covered with carbohydrates gives an indication that these unique molecules are of tremendous importance to life with impacts in health and disease. One example of the significance of carbohydrates is found in the blood types that are determined by minor differences in the structure of the glycans on the surface of red blood cells. Other examples include
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Spheroid Trapping and Calcium Spike Estimation Techniques toward Automation of 3D Culture. SLAS Technol. (IF 2.174) Pub Date : 2020-07-16 Kenneth Ndyabawe,Mark Haidekker,Amish Asthana,William S Kisaalita
We present a spheroid trapping device, compatible with traditional tissue culture plates, to confine microtissues in a small area and allow suspension cultures to be treated like adherent cultures with minimal loss of spheroids due to aspiration. We also illustrate an automated morphology-independent procedure for cell recognition, segmentation, and a calcium spike detection technique for high-throughput
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The Diagnostic Accuracy of Liquid Biopsy in EGFR-Mutated NSCLC: A Systematic Review and Meta-Analysis of 40 Studies. SLAS Technol. (IF 2.174) Pub Date : 2020-07-13 Naiqun Wang,Xiaolian Zhang,Feilong Wang,Min Zhang,Bo Sun,Weihua Yin,Shaorong Deng,Ying Wan,Wei Lu
Epidermal growth factor receptor (EGFR) mutations are the most common carcinogenic driver mutations in non-small-cell lung cancer (NSCLC) patients, while invasive tissue biopsy has certain inherent defects. PubMed, Ovid Medline, Embase, and the Cochrane Library were systematically searched on January 4, 2020, using the keywords “liquid biopsy,” “EGFR,” and “NSCLC.” The pooled sensitivity and specificity
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Preparation and Application of Nanosensor in Safeguarding Heparin Supply Chain. SLAS Technol. (IF 2.174) Pub Date : 2020-06-26 Yiling Bi,Khoi Dang Le,Gurusankar Ramamoorthy,Balagurunathan Kuberan
Heparin has been in clinical use as an anticoagulant for the last eight decades and used worldwide in more than 100 million medical procedures every year. This lifesaving drug is predominantly obtained from ~700 million pig intestines or bovine organs through millions of small and medium-sized slaughterhouses. However, the preparations from animal sources have raised many safety concerns, including
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Role of Digital Microfluidics in Enabling Access to Laboratory Automation and Making Biology Programmable. SLAS Technol. (IF 2.174) Pub Date : 2020-06-25 Varun B Kothamachu,Sabrina Zaini,Federico Muffatto
Digital microfluidics (DMF) is a liquid handling technique that has been demonstrated to automate biological experimentation in a low-cost, rapid, and programmable manner. This review discusses the role of DMF as a “digital bioconverter”—a tool to connect the digital aspects of the design–build–learn cycle with the physical execution of experiments. Several applications are reviewed to demonstrate
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An Intestine-on-a-Chip Model of Plug-and-Play Modularity to Study Inflammatory Processes. SLAS Technol. (IF 2.174) Pub Date : 2020-06-24 Linda Gijzen,Diego Marescotti,Elisa Raineri,Arnaud Nicolas,Henriette L Lanz,Diego Guerrera,Remko van Vught,Jos Joore,Paul Vulto,Manuel C Peitsch,Julia Hoeng,Giuseppe Lo Sasso,Dorota Kurek
Development of efficient drugs and therapies for the treatment of inflammatory conditions in the intestine is often hampered by the lack of reliable, robust, and high-throughput in vitro and in vivo models. Current models generally fail to recapitulate key aspects of the intestine, resulting in low translatability to the human situation. Here, an immunocompetent 3D perfused intestine-on-a-chip platform
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Flow Chemistry System for Carbohydrate Analysis by Rapid Labeling of Saccharides after Glycan Hydrolysis. SLAS Technol. (IF 2.174) Pub Date : 2020-06-19 Wei-Ting Hung,Yi-Ting Chen,Chung-Hsuan Chen,Yuan Chuan Lee,Jim-Min Fang,Wen-Bin Yang
This study demonstrates the utilization of a flow chemistry system for continuous glycan hydrolysis and saccharide labeling to assist with the existing methods in glycan structural analysis. Acidic hydrolysis of glycans could be accelerated in a flow system. Aldoses and α-ketoacid-type saccharides were effectively labeled with naphthalene-2,3-diamine (NADA) at 60 °C for 10 min to form the fluorescent
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Induced Pluripotent Stem Cells on a Chip: A Self-Contained, Accessible, Pipette-less iPSC Culturing and Differentiation Kit. SLAS Technol. (IF 2.174) Pub Date : 2020-06-19 Patrick McMinn,David J Guckenberger,David J Beebe
Over the past decade, induced pluripotent stem cells (iPSCs) have become a major focus of stem cell and developmental biology research, offering researchers a clinically relevant source of cells that are amenable to genetic engineering approaches. Though stem cells are promising for both research and commercial endeavors, iPSC-based assays require tedious protocols that include complex treatments,
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An Automated Tube Labeler for High-Throughput Purification Laboratories. SLAS Technol. (IF 2.174) Pub Date : 2020-06-07 Jeffrey Y Pan,David Chang-Yen,David P Blanchard,Wil Lam,Philip A Searle
Centralized high-throughput purification laboratories routinely produce large numbers of test tubes with fractions containing the purified compounds of interest interspersed with test tubes containing fractions collected from undesired peaks. Because the next step after purification entails the removal of the solvent in a centrifugal evaporator with multiple sample positions per rotor, select test
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Core Hairpin Structure of SpCas9 sgRNA Functions in a Sequence- and Spatial Conformation-Dependent Manner. SLAS Technol. (IF 2.174) Pub Date : 2020-06-03 Mingjun Jiang,Yanzhen Ye,Juan Li
The clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system is a widely used genome-editing tool with great clinical potential. However, its application is limited because of low editing efficiency of some target sequences and off-target effects. As this system contains only the Cas9 protein and a single-guide RNA (sgRNA; engineered from crRNA and tracrRNA), the structure and
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Locating Methyl-Etherified and Methyl-Esterified Uronic Acids in the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II. SLAS Technol. (IF 2.174) Pub Date : 2020-05-29 Malcolm A O'Neill,Ian Black,Breeanna Urbanowicz,Vivek Bharadwaj,Mike Crowley,Sabina Koj,Maria J Peña
Rhamnogalacturonan II (RG-II) is a structurally complex pectic polysaccharide that exists as a borate ester cross-linked dimer in the cell walls of all vascular plants. The glycosyl sequence of RG-II is largely conserved, but there is evidence that galacturonic acid (GalA) methyl etherification and glucuronic acid (GlcA) methyl esterification vary in the A sidechain across plant species. Methyl esterification
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High-Throughput Analysis of Fluorescently Labeled N-Glycans Derived from Biotherapeutics Using an Automated LC-MS-Based Solution. SLAS Technol. (IF 2.174) Pub Date : 2020-05-27 Ximo Zhang,Corey E Reed,Robert E Birdsall,Ying Qing Yu,Weibin Chen
Protein glycosylation can impact the efficacy and safety of biotherapeutics and therefore needs to be well characterized and monitored throughout the drug product life cycle. Glycosylation is commonly assessed by fluorescent labeling of released glycans, which provides comprehensive information of the glycoprofile but can be resource-intensive regarding sample preparation, data acquisition, and data
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Toward Automated Additive Manufacturing of Living Bio-Tubes Using Ring-Shaped Building Units. SLAS Technol. (IF 2.174) Pub Date : 2020-05-26 Kali L Manning,Jacob Feder,Marianne Kanellias,John Murphy,Jeffrey R Morgan
Tissue engineering has been largely confined to academic research institutions with limited success in commercial settings. To help address this issue, more work is needed to develop new automated manufacturing processes for tissue-related technologies. In this article, we describe the automation of the funnel-guide, an additive manufacturing method that uses living tissue rings as building units to
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Saturation Transfer Difference in Characterization of Glycosaminoglycan-Protein Interactions. SLAS Technol. (IF 2.174) Pub Date : 2020-05-26 William P Vignovich,Vitor H Pomin
Novel methods in nuclear magnetic resonance (NMR) spectroscopy have recently been developed to investigate the binding properties of intermolecular complexes endowed with biomedical functions. Among these methods is the saturation transfer difference (STD), which enables the mapping of specific binding motifs of functional ligands. STD can efficiently uncover the specific and preferential binding sites
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Hybrid Blood Collection Tubes: Combining the Best Attributes of Glass and Plastic for Safety and Shelf life. SLAS Technol. (IF 2.174) Pub Date : 2020-05-19 Christopher M Weikart,Adam P Breeland,Matt S Wills,Martin E Baltazar-Lopez
SiO2 Medical Products, Inc. developed hybrid blood collection tubes (BCTs) that combine the breakage resistance of plastic and a shelf life approaching that of glass. These blended attributes provide improved BCT safety and reliability for patients and clinical workers. A shelf life of at least 2 y with less than 10% draw volume variation was demonstrated on evacuated hybrid BCTs, which is approximately
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Microfluidic Pneumatic Printed Sandwiched Microdroplet Array for High-Throughput Enzymatic Reaction and Screening. SLAS Technol. (IF 2.174) Pub Date : 2020-05-14 Yang Sun,Gang Wang,Zhi Jing,Jingting Liang,Jiajie Sui,Jinzhen Fan,Jiannan Li
High-throughput enzyme screening for desired functionality is highly demanded. This paper utilizes a newly developed microfluidic pneumatic printing platform for high-throughput enzyme screening applications. The novel printing platform can achieve distinct features including a disposable cartridge, which avoids crosstalk; a flexible cartridge design, allowing for integration of multiple channels;
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Simplifying Glycan Profiling through a High-Throughput Micropermethylation Strategy. SLAS Technol. (IF 2.174) Pub Date : 2020-05-04 Asif Shajahan,Nitin T Supekar,Digantkumar Chapla,Christian Heiss,Kelley W Moremen,Parastoo Azadi
Glycoproteins play key roles in various molecular and cellular functions and are among the most difficult to analyze biomolecules on account of their microheterogeneity, non-template-driven synthesis, and low abundances. The stability, serum half-life, immunogenicity, and biological activity of therapeutic glycoproteins, including antibodies, vaccines, and biomarkers, are regulated by their glycosylation
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Centrifugal Generation of Droplet-Based 3D Cell Cultures. SLAS Technol. (IF 2.174) Pub Date : 2020-04-30 Jacqueline A De Lora,Jason L Velasquez,Nick J Carroll,James P Freyer,Andrew P Shreve
Quickly and easily producing uniform populations of microsphere-based 3D cell cultures using droplet-based templating methods has the potential to enable widespread use of such platforms in drug discovery or cancer research. Here, we advance the design of centrifuge-based droplet generation devices, describe the use of this platform for droplet generation with controlled cell occupancy, and demonstrate
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Integration of Acoustic Liquid Handling into Quantitative Analysis of Biological Matrix Samples. SLAS Technol. (IF 2.174) Pub Date : 2020-04-30 Linna Wang,Gerard Dalglish,Zheng Ouyang,Donata Gloria David-Brown,Camelia Chiriac,Jia Duo,Alexander Kozhich,Qin C Ji,Jon E Peterson
Acoustic liquid handlers deliver small volumes (nL-µL) of multiple fluid types with accuracy and dynamic viscosity profiling. They are widely used in the pharmaceutical industry with applications extending from high-throughput screening in compound management to gene expression sequencing, genomic and epigenetic assays, and cell-based assays. The capability of the Echo to transfer small volumes of
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Microflow-Based Device for In Vitro and Ex Vivo Drug Permeability Studies. SLAS Technol. (IF 2.174) Pub Date : 2020-04-30 Samu Hemmilä,Marika Ruponen,Elisa Toropainen,Unni Tengvall-Unadike,Arto Urtti,Pasi Kallio
This paper presents a novel microflow-based concept for studying the permeability of in vitro cell models or ex vivo tissues. Using the proposed concept, we demonstrate how to maintain physiologically relevant test conditions and produce highly reproducible permeability values for a range (31) of drug compounds. The apparent permeability coefficients (Papp) showed excellent correlation (0.89) with
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Diffusion MRI Derived per Area Vessel Density as a Surrogate Biomarker for Detecting Viral Hepatitis B-Induced Liver Fibrosis: A Proof-of-Concept Study. SLAS Technol. (IF 2.174) Pub Date : 2020-04-15 Ben-Heng Xiao,Hua Huang,Li-Fei Wang,Shi-Wen Qiu,Sheng-Wen Guo,Yì Xiáng J Wáng
Liver vessel density can be evaluated by an imaging biomarker diffusion-derived vessel density (DDVD): DDVD/area(b0b2) = Sb0/ROIarea0 – Sb2/ROIarea2, where Sb0 and Sb2 refer to the liver signal when b is 0 or 2 (s/mm2); ROIarea0 and ROIarea2 refer to the region of interest (ROI) on b = 0 or 2 images; and Sb2 may be replaced by Sb15 (b = 15). This concept was validated in this study. Liver diffusion
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Analytical Techniques for Determining the Role of Domain of Unknown Function 579 Proteins in the Synthesis of O-Methylated Plant Polysaccharides. SLAS Technol. (IF 2.174) Pub Date : 2020-03-23 Peter J Smith,Malcolm A O'Neill,Jason Backe,William S York,Maria J Peña,Breeanna R Urbanowicz
Matrix polysaccharides are a diverse group of structurally complex carbohydrates and account for a large portion of the biomass consumed as food or used to produce fuels and materials. Glucuronoxylan and arabinogalactan protein are matrix glycans that have sidechains decorated with 4-O-methyl glucuronosyl residues. Methylation is a key determinant of the physical properties of these wall glycopolymers
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Impedance-Based Single-Cell Pipetting. SLAS Technol. (IF 2.174) Pub Date : 2020-03-14 David Bonzon,Georges Muller,Jean-Baptiste Bureau,Nicolas Uffer,Nicolas Beuchat,Yann Barrandon,Philippe Renaud
Many biological methods are based on single-cell isolation. In single-cell line development, the gold standard involves the dilution of cells by means of a pipet. This process is time-consuming as it is repeated over several weeks to ensure clonality. Here, we report the modeling, designing, and testing of a disposable pipet tip integrating a cell sensor based on the Coulter principle. We investigate
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A Comparative Analysis of Methods for Quantitation of Sugars during the Corn-to-Ethanol Fermentation Process. SLAS Technol. (IF 2.174) Pub Date : 2020-02-28 Sarah R Bilskey,Samantha A Olendorff,Karolina Chmielewska,Kevin R Tucker
The quantitation of sugars, including glucose, the primary fermentable sugar; maltose (DP2); and maltotriose (DP3), is a standard procedure during the corn-to-ethanol fermentation process. The quantitation of glucose by the Megazyme Assay utilizing glucose oxidase and peroxidase enzymes (GOPOD) and UV-Vis detection, high-performance liquid chromatography with refractive index detection (HPLC-RID),
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Traceable Impedance-Based Dispensing and Cloning of Living Single Cells. SLAS Technol. (IF 2.174) Pub Date : 2020-02-18 Georges Muller,David Bonzon,Stéphanie Claudinot,Ariane Rochat,Philippe Renaud,Yann Barrandon
Single-cell cloning is essential in stem cell biology, cancer research, and biotechnology. Regulatory agencies now require an indisputable proof of clonality that current technologies do not readily provide. Here, we report a one-step cloning method using an engineered pipet combined with an impedance-based sensing tip. This technology permits the efficient and traceable isolation of living cells,
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Cell-Based In Vitro Assay Automation: Balancing Technology and Data Reproducibility/Predictability. SLAS Technol. (IF 2.174) Pub Date : 2020-01-31 Brande Thomas-Fowlkes,Steven Cifelli,Sarah Souza,Richard Visconti,Alice Struck,Adam Weinglass,Mary Jo Wildey
G-protein-coupled receptors (GPCRs) are modulated by many marketed drugs, and as such, they continue to be key targets for drug discovery and development. Many GPCR targets at Merck Research Laboratories (MRL) are profiled using homogenous time-resolved fluorescence (HTRF) inositol monophosphate (IP-1) cell-based functional assays using adherent cells in 384-well microplates. Due to discrepancies observed
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Ratiometric Nanoviscometers: Applications for Measuring Cellular Physical Properties in 3D Cultures. SLAS Technol. (IF 2.174) Pub Date : 2020-01-30 Charles McRae White,Mark A Haidekker,William S Kisaalita
New insights into the biomechanical properties of cells are revealing the importance of these properties and how they relate to underlying molecular, architectural, and behavioral changes associated with cell state and disease processes. However, the current understanding of how these in vitro biomechanical properties are associated with in vivo processes has been developed based on the traditional